AT5G09660

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009925
Physical Location & Seq
Reverse (-)
2993411 .. 2995784
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G09660.2

Sequence Viewer

Length: 1065 bp
ATGGAGTTTCGTGGAGATGCCAACCAGAGGATTGCTAGGATTTCAGCTCATCTCACTCCTCAGATGGAGGCCAAGAACTCTGTAATCGGACGGGAAAACTGCAGAGCTAAAGGTGGTAATCCAGGATTCAAAGTAGCAATTCTTGGAGCTGCAGGTGGAATTGGACAATCTTTATCTTTGCTGATGAAGATGAACCCTCTTGTCTCTTTACTTCATCTCTACGATGTTGTCAATGCTCCTGGCGTCACTGCTGACGTCAGTCATATGGACACTGGAGCTGTTGTCCGCGGGTTCTTGGGAGCGAAGCAGCTTGAGGACGCGCTAACGGGTATGGATCTTGTGATCATACCAGCCGGTATACCGAGGAAACCAGGGATGACCCGCGATGATCTGTTTAAAATCAATGCTGGGATTGTTAAAACACTATGTGAAGGTGTAGCAAAATGTTGTCCTAATGCTATTGTCAACTTGATCAGCAACCCTGTGAACTCTACTGTCCCCATTGCCGCTGAGGTTTTCAAGAAAGCTGGAACTTATGATCCTAAGAAGCTCCTTGGAGTTACTACACTCGATGTTGCTCGTGCCAACACATTTGTGGCAGAAGTTCTTGGCCTTGATCCAAGAGAAGTCGATGTGCCAGTAGTTGGGGGACACGCCGGAGTCACAATCTTGCCACTACTGTCACAGGTTAAACCTCCTAGCAGCTTCACACCTCAAGAAATTGAGTACCTGACAAACCGGATTCAAAATGGTGGAACTGAAGTTGTGGAGGCAAAAGCTGGAGCTGGTTCTGCAACACTTTCAATGGCATATGCTGCAGCCAAGTTTGCAGATGCTTGCCTTCGCGGGTTAAGAGGAGATGCGAATGTCGTAGAATGCTCTTTTGTTGCTTCACAGGTGACAGAATTAGCTTTCTTTGCAACAAAAGTGCGCCTTGGCCGTACAGGAGCAGAGGAAGTGTATCAGCTTGGACCCTTAAACGAATACGAAAGGATTGGTCTGGAGAAAGCAAAAGATGAATTAGCCGGAAGTATTCAGAAAGGTGTTGAATTCATCAGAAAATGA

Protein Analysis

354

Amino Acids

37.37

Weight (kDa)

8.14

Isoelectric Point (pI)

21.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 23 - 165 2.3e-46 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 167 - 330 1.3e-45 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09660 AT5G09660 AT5G09660 AT5G09660
fragaria_vesca FvH4_2g02490 FvH4_2g02490
malus_domestica MD05G1112700.v1.1
prunus_persica Prupe.8G157300_v2.0.a1
pyrus_communis pycom05g10810 pycom10g09980
rosa_chinensis RchiOBHm_Chr6g0246321
rosa_laevigata RLG00000015263
rosa_multiflora Rmu_sc0001066.1_g000009
rosa_roxburghii Rroxscaffold_7G00214680
rosa_rugosa Rorug05G0520300.1
rosa_samantha Rh6AG034500 Rh6BG029600 Rh6CG030000 Rh6DG028900
rosa_wichuraiana Rw6G002790 Rw6G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 1 cut(s) 358
AccII CGCG 4 cut(s) 288, 320, 384, 846
AciI CCGC 5 cut(s) 286, 288, 382, 507, 846
AclWI GGATC 3 cut(s) 342, 533, 611
AcoI YGGCCR 1 cut(s) 937
AcsI RAATTY 1 cut(s) 1049
AcuI CTGAAG 1 cut(s) 780
AcyI GRCGYC 2 cut(s) 243, 255
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 2 cut(s) 728, 943
AfiI CCNNNNNNNGG 2 cut(s) 27, 644
AgsI TTSAA 5 cut(s) 130, 520, 746, 804, 1049
AjnI CCWGG 3 cut(s) 121, 238, 370
AleI CACNNNNGTG 1 cut(s) 593
AloI GAACNNNNNNTCC 2 cut(s) 523, 555
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 3 cut(s) 342, 533, 611
AoxI GGCC 3 cut(s) 69, 610, 937
ApeKI GCWGC 5 cut(s) 149, 307, 702, 815, 818
ApoI RAATTY 1 cut(s) 1049
AspLEI GCGC 2 cut(s) 322, 933
AspS9I GGNCC 1 cut(s) 971
AsuHPI GGTGA 1 cut(s) 910
AvaII GGWCC 1 cut(s) 971
BauI CACGAG 1 cut(s) 579
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 5 cut(s) 136, 319, 714, 802, 830
BccI CCATC 1 cut(s) 58
BceAI ACGGC 1 cut(s) 924
BciT130I CCWGG 3 cut(s) 123, 240, 372
BclI TGATCA 2 cut(s) 342, 471
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 2 cut(s) 36, 699
BfmI CTRYAG 3 cut(s) 100, 150, 816
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 6 cut(s) 150, 308, 507, 703, 816, 819
BlsI GCNGC 6 cut(s) 151, 309, 508, 704, 817, 820
Bme1390I CCNGG 3 cut(s) 123, 240, 372
Bme18I GGWCC 1 cut(s) 971
BmgT120I GGNCC 1 cut(s) 971
BmiI GGNNCC 1 cut(s) 973
BmrFI CCNGG 3 cut(s) 123, 240, 372
BmsI GCATC 3 cut(s) 7, 823, 850
BoxI GACNNNNGTC 1 cut(s) 258
BpmI CTGGAG 3 cut(s) 294, 801, 1022
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 332, 699
BsaHI GRCGYC 2 cut(s) 243, 255
BsaJI CCNNGG 5 cut(s) 286, 362, 371, 553, 934
BsaWI WCCGGW 1 cut(s) 738
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 644
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 2 cut(s) 277, 638
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 3 cut(s) 123, 240, 372
BseDI CCNNGG 5 cut(s) 286, 362, 371, 553, 934
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 2 cut(s) 27, 644
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 2 cut(s) 74, 501
BseNI ACTGG 2 cut(s) 277, 638
BseRI GAGGAG 2 cut(s) 48, 870
BseXI GCAGC 5 cut(s) 136, 319, 714, 802, 830
BseYI CCCAGC 1 cut(s) 407
Bsh1236I CGCG 4 cut(s) 288, 320, 384, 846
BshFI GGCC 3 cut(s) 71, 612, 939
BsiSI CCGG 4 cut(s) 354, 657, 739, 1026
BslFI GGGAC 2 cut(s) 482, 663
BslI CCNNNNNNNGG 2 cut(s) 27, 644
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 2 cut(s) 482, 663
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 3 cut(s) 71, 612, 939
Bsp143I GATC 6 cut(s) 334, 342, 388, 471, 538, 616
BspACI CCGC 5 cut(s) 286, 288, 382, 507, 846
BspANI GGCC 3 cut(s) 71, 612, 939
BspCNI CTCAG 2 cut(s) 73, 502
BspFNI CGCG 4 cut(s) 288, 320, 384, 846
BspLI GGNNCC 1 cut(s) 973
BspMAI CTGCAG 3 cut(s) 104, 154, 820
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 3 cut(s) 342, 533, 611
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 2 cut(s) 277, 638
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 5 cut(s) 286, 362, 371, 553, 934
BssMI GATC 6 cut(s) 334, 342, 388, 471, 538, 616
BssNAI GTATAC 1 cut(s) 359
BssNI GRCGYC 2 cut(s) 243, 255
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 2 cut(s) 553, 934
Bst1107I GTATAC 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 123, 240, 372
Bst4CI ACNGT 2 cut(s) 496, 681
BstACI GRCGYC 2 cut(s) 243, 255
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 1 cut(s) 838
BstDEI CTNAG 3 cut(s) 60, 510, 543
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 381
BstFNI CGCG 4 cut(s) 288, 320, 384, 846
BstHHI GCGC 2 cut(s) 322, 933
BstKTI GATC 6 cut(s) 337, 345, 391, 474, 541, 619
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 6 cut(s) 334, 342, 388, 471, 538, 616
BstMWI GCNNNNNNNGC 4 cut(s) 791, 815, 827, 917
BstNI CCWGG 3 cut(s) 123, 240, 372
BstPAI GACNNNNGTC 1 cut(s) 258
BstSCI CCNGG 3 cut(s) 121, 238, 370
BstSFI CTRYAG 3 cut(s) 100, 150, 816
BstUI CGCG 4 cut(s) 288, 320, 384, 846
BstV1I GCAGC 5 cut(s) 136, 319, 714, 802, 830
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BstZ17I GTATAC 1 cut(s) 359
BsuRI GGCC 3 cut(s) 71, 612, 939
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 381
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 246, 270
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 838
CfoI GCGC 2 cut(s) 322, 933
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 971
Cfr42I CCGCGG 1 cut(s) 289
CseI GACGC 2 cut(s) 232, 326
Csp6I GTAC 2 cut(s) 727, 942
CviQI GTAC 2 cut(s) 727, 942
DdeI CTNAG 3 cut(s) 60, 510, 543
DpnI GATC 6 cut(s) 336, 344, 390, 473, 540, 618
DpnII GATC 6 cut(s) 334, 342, 388, 471, 538, 616
DraI TTTAAA 1 cut(s) 397
DraIII CACNNNGTG 1 cut(s) 428
EaeI YGGCCR 1 cut(s) 937
Eco130I CCWWGG 2 cut(s) 553, 934
Eco47I GGWCC 1 cut(s) 971
Eco57I CTGAAG 1 cut(s) 780
EcoRI GAATTC 1 cut(s) 1049
EcoRII CCWGG 3 cut(s) 121, 238, 370
EcoT14I CCWWGG 2 cut(s) 553, 934
ErhI CCWWGG 2 cut(s) 553, 934
FaiI YATR 9 cut(s) 264, 266, 332, 347, 359, 427, 537, 811, 813
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 2 cut(s) 482, 663
FauI CCCGC 3 cut(s) 281, 389, 839
FauNDI CATATG 2 cut(s) 264, 811
FbaI TGATCA 2 cut(s) 342, 471
FblI GTMKAC 1 cut(s) 358
Fnu4HI GCNGC 6 cut(s) 150, 308, 507, 703, 816, 819
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 6 cut(s) 150, 308, 507, 703, 816, 819
FspBI CTAG 2 cut(s) 36, 699
GlaI GCGC 2 cut(s) 321, 932
GluI GCNGC 6 cut(s) 150, 308, 507, 703, 816, 819
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 3 cut(s) 294, 801, 1022
HaeIII GGCC 3 cut(s) 71, 612, 939
HapII CCGG 4 cut(s) 354, 657, 739, 1026
HgaI GACGC 2 cut(s) 232, 326
HhaI GCGC 2 cut(s) 322, 933
Hin1I GRCGYC 2 cut(s) 243, 255
Hin6I GCGC 2 cut(s) 320, 931
HinP1I GCGC 2 cut(s) 320, 931
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 3 cut(s) 126, 660, 742
HpaII CCGG 4 cut(s) 354, 657, 739, 1026
HphI GGTGA 1 cut(s) 910
Hpy166II GTNNAC 3 cut(s) 359, 466, 487
Hpy188I TCNGA 4 cut(s) 63, 89, 1038, 1058
Hpy188III TCNNGA 3 cut(s) 520, 716, 1001
Hpy8I GTNNAC 3 cut(s) 359, 466, 487
HpyAV CCTTC 2 cut(s) 425, 851
HpyCH4III ACNGT 2 cut(s) 496, 681
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 6 cut(s) 102, 152, 794, 818, 830, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 791, 815, 827, 917
HpyF3I CTNAG 3 cut(s) 60, 510, 543
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 2 cut(s) 243, 255
HspAI GCGC 2 cut(s) 320, 931
Ksp22I TGATCA 2 cut(s) 342, 471
KspI CCGCGG 1 cut(s) 289
Kzo9I GATC 6 cut(s) 334, 342, 388, 471, 538, 616
LmnI GCTCC 7 cut(s) 146, 241, 275, 299, 555, 782, 947
Lsp1109I GCAGC 5 cut(s) 136, 319, 714, 802, 830
LweI GCATC 3 cut(s) 7, 823, 850
MaeI CTAG 2 cut(s) 36, 699
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 5 cut(s) 244, 559, 661, 681, 898
MalI GATC 6 cut(s) 336, 344, 390, 473, 540, 618
MboI GATC 6 cut(s) 334, 342, 388, 471, 538, 616
MboII GAAGA 1 cut(s) 199
MflI RGATCY 1 cut(s) 334
MluCI AATT 6 cut(s) 138, 159, 720, 905, 1019, 1049
MlyI GAGTC 1 cut(s) 669
MseI TTAA 5 cut(s) 396, 417, 690, 851, 977
MslI CAYNNNNRTG 1 cut(s) 593
MspA1I CMGCKG 2 cut(s) 288, 509
MspI CCGG 4 cut(s) 354, 657, 739, 1026
MspR9I CCNGG 3 cut(s) 123, 240, 372
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 3 cut(s) 123, 240, 372
MvnI CGCG 4 cut(s) 288, 320, 384, 846
MwoI GCNNNNNNNGC 4 cut(s) 791, 815, 827, 917
NdeI CATATG 2 cut(s) 264, 811
NdeII GATC 6 cut(s) 334, 342, 388, 471, 538, 616
NlaIV GGNNCC 1 cut(s) 973
NmuCI GTSAC 4 cut(s) 244, 661, 681, 898
OliI CACNNNNGTG 1 cut(s) 593
PaqCI CACCTGC 1 cut(s) 143
PctI GAATGC 1 cut(s) 881
PfeI GAWTC 2 cut(s) 126, 742
PflMI CCANNNNNTGG 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 6 cut(s) 151, 309, 508, 704, 817, 820
PleI GAGTC 1 cut(s) 668
PpsI GAGTC 1 cut(s) 668
PshAI GACNNNNGTC 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 121, 238, 370
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 3 cut(s) 121, 238, 370
PspN4I GGNNCC 1 cut(s) 973
PspPI GGNCC 1 cut(s) 971
PstI CTGCAG 3 cut(s) 104, 154, 820
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 2 cut(s) 728, 943
RsaNI GTAC 2 cut(s) 727, 942
RseI CAYNNNNRTG 1 cut(s) 593
SacII CCGCGG 1 cut(s) 289
SaqAI TTAA 5 cut(s) 396, 417, 690, 851, 977
SatI GCNGC 6 cut(s) 150, 308, 507, 703, 816, 819
Sau3AI GATC 6 cut(s) 334, 342, 388, 471, 538, 616
Sau96I GGNCC 1 cut(s) 971
SchI GAGTC 1 cut(s) 669
ScrFI CCNGG 3 cut(s) 123, 240, 372
SfaNI GCATC 3 cut(s) 7, 823, 850
SfcI CTRYAG 3 cut(s) 100, 150, 816
Sfr303I CCGCGG 1 cut(s) 289
SgrBI CCGCGG 1 cut(s) 289
SinI GGWCC 1 cut(s) 971
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 2 cut(s) 311, 714
SmoI CTYRAG 2 cut(s) 311, 714
Sse9I AATT 6 cut(s) 138, 159, 720, 905, 1019, 1049
SsiI CCGC 5 cut(s) 286, 288, 382, 507, 846
SspMI CTAG 2 cut(s) 36, 699
StyD4I CCNGG 3 cut(s) 121, 238, 370
StyI CCWWGG 2 cut(s) 553, 934
TaaI ACNGT 2 cut(s) 496, 681
TaiI ACGT 1 cut(s) 258
TaqI TCGA 2 cut(s) 570, 630
TasI AATT 6 cut(s) 138, 159, 720, 905, 1019, 1049
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 2 cut(s) 126, 742
Tru1I TTAA 5 cut(s) 396, 417, 690, 851, 977
Tru9I TTAA 5 cut(s) 396, 417, 690, 851, 977
TscAI CASTG 2 cut(s) 253, 277
TseFI GTSAC 4 cut(s) 244, 661, 681, 898
TseI GCWGC 5 cut(s) 149, 307, 702, 815, 818
Tsp45I GTSAC 4 cut(s) 244, 661, 681, 898
TspDTI ATGAA 5 cut(s) 200, 203, 206, 1032, 1042
TspRI CASTG 2 cut(s) 253, 277
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 971
XapI RAATTY 1 cut(s) 1049
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmiI GTMKAC 1 cut(s) 358
XspI CTAG 2 cut(s) 36, 699
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.