pycom05g10810

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009925
Physical Location & Seq
Forward (+)
14264127 .. 14266619
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g10810.2

Sequence Viewer

Length: 1140 bp
ATGCAAGAAGGTTGGCTCGGTACACTTGAAAACTCACTATCCCTTTGTCTGAAAAAAAGCACAGAGAAAATGGAGTCGAGCGTGGAAGCTAATGGACGCATTGCAAGGATTTCAGCTCATCTGTTTCCACCAAATTCCCAGGTGGAGGATGGTTCTGCTCTGAGCAGAGGTGATTGCAGAGCCAAGGGCGGGGCGCCGGGCTTTAAAGTGGCGATTGTGGGTGCTGCTGGAGGCATTGGCCAACCTCTTGCAATGCTCATGAAGATGAACCCACTGGTTTCAGTTCTTCATCTCTATGATGTCGTTAATGCCCCCGGTGTCACAGCTGACATTAGCCACATGGATACCGGAGCTGTGGTGCGCGGTTTCTTGGGGCAGCCACAGCTTGAGAGCGCTCTCACGGGCATAGACCTTGTGATCATACCTGCCGGTGTTCCAAGGAAGCCTGGAATGACAAGGGATGATCTTTTCAAAATAAACGCGGGAATTGTCAGGACCATCTGTGAAGGAATTGCGAAAGCCTGTCCTAAGGCAATCGTCAACTTGATCAGTAATCCAGTGAACTCGACAGTTCCTATTGCAGCAGAGGTTTTCAAGAAAGCCGGGACTTACGACCCAAAGCGTCTTCTAGGAGTCACGATGCTTGATGTCGTGAGAGCTAATACTTTTGTTGCAGAAGTTCTTGGACTTGATCCTAGGGAAGTCGATGTTCCAGTTGTTGGTGGTCATGCCGGAGTGACCATTTTGCCGCTTCTGTCACAGGTCAAGCCTCCTTGCTCCTTCACCAAAGAAGAAACAGAATACCTAACAAACCGGATTCAAAACGGTGGAACAGAAGTTGTTGAGGCAAAAGCTGGGGCTGGTTCGGCAACACTGTCAATGGCATATGCAGCTGTGAAATTTGCGGATGCATGCCTCCGCGGCTTGAGAGGAGATGCTGGTGTTGTTGAATGCGCTTTTGTTGCCTCTGAGGTAACCGAACTTCCTTTCTTCGCAACCAAGGTCCGGCTTGGTCGTGGCGGAGCAGAGGAAATCTACCAACTCGGGCCTCTGAATGAGTATGAGAGGGTTGGATTAGAGAAGGCGAAAACGGAGTTAGCAGCAAGCATCCAGAAGGGGATTTCGTTCATCAAGAAGTGA

Protein Analysis

380

Amino Acids

39.85

Weight (kDa)

7.55

Isoelectric Point (pI)

27.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09660 AT5G09660 AT5G09660 AT5G09660
fragaria_vesca FvH4_2g02490 FvH4_2g02490
malus_domestica MD05G1112700.v1.1
prunus_persica Prupe.8G157300_v2.0.a1
pyrus_communis pycom05g10810 pycom10g09980
rosa_chinensis RchiOBHm_Chr6g0246321
rosa_laevigata RLG00000015263
rosa_multiflora Rmu_sc0001066.1_g000009
rosa_roxburghii Rroxscaffold_7G00214680
rosa_rugosa Rorug05G0520300.1
rosa_samantha Rh6AG034500 Rh6BG029600 Rh6CG030000 Rh6DG028900
rosa_wichuraiana Rw6G002790 Rw6G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 719
AccII CGCG 3 cut(s) 363, 482, 921
AciI CCGC 8 cut(s) 189, 363, 482, 749, 905, 919, 921, 1020
AclWI GGATC 1 cut(s) 686
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 2 cut(s) 133, 899
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 1 cut(s) 22
AfeI AGCGCT 1 cut(s) 394
AfiI CCNNNNNNNGG 4 cut(s) 145, 189, 719, 1005
AgsI TTSAA 5 cut(s) 29, 472, 595, 821, 950
AjnI CCWGG 2 cut(s) 138, 445
AluBI AGCT 8 cut(s) 89, 116, 326, 353, 385, 659, 854, 893
AluI AGCT 8 cut(s) 89, 116, 326, 353, 385, 659, 854, 893
AlwI GGATC 1 cut(s) 686
Ama87I CYCGRG 1 cut(s) 1043
Aor51HI AGCGCT 1 cut(s) 394
AoxI GGCC 2 cut(s) 238, 1046
ApeKI GCWGC 5 cut(s) 224, 376, 581, 890, 1100
ApoI RAATTY 2 cut(s) 133, 899
AspA2I CCTAGG 1 cut(s) 695
AspLEI GCGC 4 cut(s) 196, 363, 395, 956
AspS9I GGNCC 3 cut(s) 495, 1003, 1046
AsuC2I CCSGG 3 cut(s) 198, 315, 604
AsuHPI GGTGA 2 cut(s) 182, 775
AvaI CYCGRG 1 cut(s) 1043
AvaII GGWCC 2 cut(s) 495, 1003
AvrII CCTAGG 1 cut(s) 695
AxyI CCTNAGG 1 cut(s) 528
BalI TGGCCA 1 cut(s) 240
BanI GGYRCC 1 cut(s) 193
BarI GAAGNNNNNNTAC 2 cut(s) 966, 998
BbsI GAAGAC 1 cut(s) 617
BbvI GCAGC 5 cut(s) 211, 388, 593, 902, 1112
BccI CCATC 2 cut(s) 143, 506
BciT130I CCWGG 2 cut(s) 140, 447
BciVI GTATCC 1 cut(s) 337
BclI TGATCA 2 cut(s) 417, 546
BcnI CCSGG 3 cut(s) 198, 315, 604
BfaI CTAG 2 cut(s) 629, 696
BfoI RGCGCY 2 cut(s) 197, 396
BfuAI ACCTGC 1 cut(s) 433
BfuI GTATCC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 921
BisI GCNGC 7 cut(s) 225, 377, 582, 749, 891, 922, 1101
BlnI CCTAGG 1 cut(s) 695
BlsI GCNGC 7 cut(s) 226, 378, 583, 750, 892, 923, 1102
Bme1390I CCNGG 5 cut(s) 140, 198, 315, 447, 604
Bme18I GGWCC 2 cut(s) 495, 1003
BmeT110I CYCGRG 1 cut(s) 1043
BmgT120I GGNCC 3 cut(s) 495, 1003, 1046
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 5 cut(s) 140, 198, 315, 447, 604
BmsI GCATC 4 cut(s) 630, 898, 925, 1116
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 249
BpuEI CTTGAG 2 cut(s) 407, 946
BpuMI CCSGG 3 cut(s) 198, 315, 604
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 7 cut(s) 138, 183, 313, 437, 695, 919, 999
BsaWI WCCGGW 2 cut(s) 347, 813
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 189, 719, 1005
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 3 cut(s) 279, 557, 713
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 2 cut(s) 99, 258
BseBI CCWGG 2 cut(s) 140, 447
BseDI CCNNGG 7 cut(s) 138, 183, 313, 437, 695, 919, 999
BseGI GGATG 4 cut(s) 154, 466, 913, 1107
BseLI CCNNNNNNNGG 4 cut(s) 145, 189, 719, 1005
BseMI GCAATG 2 cut(s) 99, 258
BseMII CTCAG 2 cut(s) 152, 960
BseNI ACTGG 3 cut(s) 279, 557, 713
BseRI GAGGAG 1 cut(s) 945
BseXI GCAGC 5 cut(s) 211, 388, 593, 902, 1112
BseYI CCCAGC 1 cut(s) 854
Bsh1236I CGCG 3 cut(s) 363, 482, 921
BshFI GGCC 2 cut(s) 240, 1048
BshNI GGYRCC 1 cut(s) 193
BsiHKCI CYCGRG 1 cut(s) 1043
BsiSI CCGG 8 cut(s) 197, 315, 348, 429, 603, 732, 814, 1006
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 4 cut(s) 145, 189, 719, 1005
BsmFI GGGAC 1 cut(s) 619
BsmI GAATGC 1 cut(s) 956
BsnI GGCC 2 cut(s) 240, 1048
BsoBI CYCGRG 1 cut(s) 1043
Bsp143I GATC 4 cut(s) 417, 463, 546, 691
BspACI CCGC 8 cut(s) 189, 363, 482, 749, 905, 919, 921, 1020
BspANI GGCC 2 cut(s) 240, 1048
BspCNI CTCAG 2 cut(s) 153, 961
BspFNI CGCG 3 cut(s) 363, 482, 921
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 195
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 1 cut(s) 686
BspT107I GGYRCC 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 99, 258
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 3 cut(s) 279, 557, 713
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 7 cut(s) 138, 183, 313, 437, 695, 919, 999
BssMI GATC 4 cut(s) 417, 463, 546, 691
BssNI GRCGYC 1 cut(s) 194
BssT1I CCWWGG 4 cut(s) 183, 437, 695, 999
Bst2UI CCWGG 2 cut(s) 140, 447
Bst4CI ACNGT 3 cut(s) 571, 827, 876
BstACI GRCGYC 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 913, 1105
BstDEI CTNAG 3 cut(s) 161, 528, 969
BstDSI CCRYGG 1 cut(s) 919
BstEII GGTNACC 1 cut(s) 973
BstF5I GGATG 4 cut(s) 154, 466, 913, 1107
BstFNI CGCG 3 cut(s) 363, 482, 921
BstH2I RGCGCY 2 cut(s) 197, 396
BstHHI GCGC 4 cut(s) 196, 363, 395, 956
BstKTI GATC 4 cut(s) 420, 466, 549, 694
BstMBI GATC 4 cut(s) 417, 463, 546, 691
BstMWI GCNNNNNNNGC 5 cut(s) 382, 866, 890, 921, 962
BstNI CCWGG 2 cut(s) 140, 447
BstNSI RCATGY 1 cut(s) 915
BstPI GGTNACC 1 cut(s) 973
BstSCI CCNGG 5 cut(s) 138, 196, 313, 445, 602
BstUI CGCG 3 cut(s) 363, 482, 921
BstV1I GCAGC 5 cut(s) 211, 388, 593, 902, 1112
BstV2I GAAGAC 1 cut(s) 617
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 2 cut(s) 240, 1048
BtgI CCRYGG 1 cut(s) 919
BtsCI GGATG 4 cut(s) 154, 466, 913, 1107
BtsIMutI CAGTG 3 cut(s) 272, 564, 872
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 913, 1105
CciI TCATGA 1 cut(s) 258
CfoI GCGC 4 cut(s) 196, 363, 395, 956
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 3 cut(s) 495, 1003, 1046
Cfr42I CCGCGG 1 cut(s) 922
CseI GACGC 2 cut(s) 105, 611
Csp6I GTAC 1 cut(s) 21
CviAII CATG 4 cut(s) 259, 340, 728, 912
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 3 cut(s) 161, 528, 969
DinI GGCGCC 1 cut(s) 195
DpnI GATC 4 cut(s) 419, 465, 548, 693
DpnII GATC 4 cut(s) 417, 463, 546, 691
DraI TTTAAA 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 238
EciI GGCGGA 1 cut(s) 1035
Eco130I CCWWGG 4 cut(s) 183, 437, 695, 999
Eco47I GGWCC 2 cut(s) 495, 1003
Eco47III AGCGCT 1 cut(s) 394
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 1043
Eco91I GGTNACC 1 cut(s) 973
EcoO65I GGTNACC 1 cut(s) 973
EcoRII CCWGG 2 cut(s) 138, 445
EcoT14I CCWWGG 4 cut(s) 183, 437, 695, 999
EcoT22I ATGCAT 1 cut(s) 913
EgeI GGCGCC 1 cut(s) 195
EheI GGCGCC 1 cut(s) 195
ErhI CCWWGG 4 cut(s) 183, 437, 695, 999
FaeI CATG 4 cut(s) 262, 343, 731, 915
FaqI GGGAC 1 cut(s) 619
FatI CATG 4 cut(s) 258, 339, 727, 911
FauI CCCGC 2 cut(s) 182, 475
FauNDI CATATG 1 cut(s) 886
FbaI TGATCA 2 cut(s) 417, 546
Fnu4HI GCNGC 7 cut(s) 225, 377, 582, 749, 891, 922, 1101
FokI GGATG 4 cut(s) 161, 473, 920, 1094
Fsp4HI GCNGC 7 cut(s) 225, 377, 582, 749, 891, 922, 1101
FspBI CTAG 2 cut(s) 629, 696
GlaI GCGC 4 cut(s) 195, 362, 394, 955
GluI GCNGC 7 cut(s) 225, 377, 582, 749, 891, 922, 1101
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 1 cut(s) 249
HaeII RGCGCY 2 cut(s) 197, 396
HaeIII GGCC 2 cut(s) 240, 1048
HapII CCGG 8 cut(s) 197, 315, 348, 429, 603, 732, 814, 1006
HgaI GACGC 2 cut(s) 105, 611
HhaI GCGC 4 cut(s) 196, 363, 395, 956
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 4 cut(s) 262, 343, 731, 915
Hin6I GCGC 4 cut(s) 194, 361, 393, 954
HinP1I GCGC 4 cut(s) 194, 361, 393, 954
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 3 cut(s) 74, 633, 817
HpaII CCGG 8 cut(s) 197, 315, 348, 429, 603, 732, 814, 1006
HphI GGTGA 2 cut(s) 182, 775
Hpy166II GTNNAC 3 cut(s) 23, 541, 562
Hpy188I TCNGA 4 cut(s) 51, 162, 970, 1053
Hpy188III TCNNGA 7 cut(s) 259, 493, 595, 637, 652, 1111, 1132
Hpy8I GTNNAC 3 cut(s) 23, 541, 562
HpyAV CCTTC 4 cut(s) 500, 790, 1075, 1108
HpyCH4III ACNGT 3 cut(s) 571, 827, 876
HpyCH4V TGCA 8 cut(s) 4, 104, 177, 251, 581, 674, 890, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 382, 866, 890, 921, 962
HpyF3I CTNAG 3 cut(s) 161, 528, 969
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 4 cut(s) 262, 343, 731, 915
HspAI GCGC 4 cut(s) 194, 361, 393, 954
KasI GGCGCC 1 cut(s) 193
Ksp22I TGATCA 2 cut(s) 417, 546
KspI CCGCGG 1 cut(s) 922
Kzo9I GATC 4 cut(s) 417, 463, 546, 691
LmnI GCTCC 3 cut(s) 350, 782, 1022
Lsp1109I GCAGC 5 cut(s) 211, 388, 593, 902, 1112
LweI GCATC 4 cut(s) 630, 898, 925, 1116
MaeI CTAG 2 cut(s) 629, 696
MaeIII GTNAC 5 cut(s) 319, 634, 736, 756, 973
MalI GATC 4 cut(s) 419, 465, 548, 693
MboI GATC 4 cut(s) 417, 463, 546, 691
MboII GAAGA 5 cut(s) 274, 278, 617, 803, 982
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 4 cut(s) 133, 486, 510, 899
MluNI TGGCCA 1 cut(s) 240
Mly113I GGCGCC 1 cut(s) 194
MlyI GAGTC 2 cut(s) 83, 642
MmeI TCCRAC 1 cut(s) 1051
Mox20I TGGCCA 1 cut(s) 240
Mph1103I ATGCAT 1 cut(s) 913
MscI TGGCCA 1 cut(s) 240
MseI TTAA 2 cut(s) 204, 306
MslI CAYNNNNRTG 2 cut(s) 263, 294
Msp20I TGGCCA 1 cut(s) 240
MspA1I CMGCKG 3 cut(s) 326, 893, 921
MspI CCGG 8 cut(s) 197, 315, 348, 429, 603, 732, 814, 1006
MspR9I CCNGG 5 cut(s) 140, 198, 315, 447, 604
Mva1269I GAATGC 1 cut(s) 956
MvaI CCWGG 2 cut(s) 140, 447
MvnI CGCG 3 cut(s) 363, 482, 921
MwoI GCNNNNNNNGC 5 cut(s) 382, 866, 890, 921, 962
NarI GGCGCC 1 cut(s) 194
NciI CCSGG 3 cut(s) 198, 315, 604
NdeI CATATG 1 cut(s) 886
NdeII GATC 4 cut(s) 417, 463, 546, 691
NlaIII CATG 4 cut(s) 262, 343, 731, 915
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 4 cut(s) 319, 634, 736, 756
NsiI ATGCAT 1 cut(s) 913
NspI RCATGY 1 cut(s) 915
PaeI GCATGC 1 cut(s) 915
PagI TCATGA 1 cut(s) 258
PctI GAATGC 1 cut(s) 956
PfeI GAWTC 1 cut(s) 817
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 7 cut(s) 226, 378, 583, 750, 892, 923, 1102
PleI GAGTC 2 cut(s) 82, 641
PluTI GGCGCC 1 cut(s) 197
PpsI GAGTC 2 cut(s) 82, 641
Psp6I CCWGG 2 cut(s) 138, 445
PspEI GGTNACC 1 cut(s) 973
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 2 cut(s) 138, 445
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 3 cut(s) 495, 1003, 1046
PvuII CAGCTG 2 cut(s) 326, 893
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 2 cut(s) 263, 294
SacII CCGCGG 1 cut(s) 922
SaqAI TTAA 2 cut(s) 204, 306
SatI GCNGC 7 cut(s) 225, 377, 582, 749, 891, 922, 1101
Sau3AI GATC 4 cut(s) 417, 463, 546, 691
Sau96I GGNCC 3 cut(s) 495, 1003, 1046
SchI GAGTC 2 cut(s) 83, 642
ScrFI CCNGG 5 cut(s) 140, 198, 315, 447, 604
SfaNI GCATC 4 cut(s) 630, 898, 925, 1116
SfoI GGCGCC 1 cut(s) 195
Sfr303I CCGCGG 1 cut(s) 922
SgrBI CCGCGG 1 cut(s) 922
SinI GGWCC 2 cut(s) 495, 1003
SmiMI CAYNNNNRTG 2 cut(s) 263, 294
SmlI CTYRAG 2 cut(s) 386, 925
SmoI CTYRAG 2 cut(s) 386, 925
SphI GCATGC 1 cut(s) 915
Sse9I AATT 4 cut(s) 133, 486, 510, 899
SsiI CCGC 8 cut(s) 189, 363, 482, 749, 905, 919, 921, 1020
SspDI GGCGCC 1 cut(s) 193
SspMI CTAG 2 cut(s) 629, 696
StyD4I CCNGG 5 cut(s) 138, 196, 313, 445, 602
StyI CCWWGG 4 cut(s) 183, 437, 695, 999
TaaI ACNGT 3 cut(s) 571, 827, 876
TaqI TCGA 3 cut(s) 77, 566, 705
TasI AATT 4 cut(s) 133, 486, 510, 899
TauI GCSGC 2 cut(s) 751, 924
TfiI GAWTC 1 cut(s) 817
Tru1I TTAA 2 cut(s) 204, 306
Tru9I TTAA 2 cut(s) 204, 306
TscAI CASTG 3 cut(s) 279, 564, 879
TseFI GTSAC 4 cut(s) 319, 634, 736, 756
TseI GCWGC 5 cut(s) 224, 376, 581, 890, 1100
Tsp45I GTSAC 4 cut(s) 319, 634, 736, 756
TspDTI ATGAA 4 cut(s) 275, 278, 281, 1117
TspGWI ACGGA 1 cut(s) 1106
TspRI CASTG 3 cut(s) 279, 564, 879
Van91I CCANNNNNTGG 1 cut(s) 719
VpaK11BI GGWCC 2 cut(s) 495, 1003
XapI RAATTY 2 cut(s) 133, 899
XceI RCATGY 1 cut(s) 915
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XmaJI CCTAGG 1 cut(s) 695
XspI CTAG 2 cut(s) 629, 696
Zsp2I ATGCAT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.