MD05G1112700.v1.1

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009925
Physical Location & Seq
Forward (+)
23131571 .. 23134300
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1112700.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGGAGTCGAGCGTGGGAGCTAATGGACGCATTGCAAAGATTTCAGCTCATCTGTTTCCACCAAATTCCCAGGTGGAGGATGGTTCTGCTTTGAGCAGAGGTGATTGCAGAGCCAAGGGCGGGGCGCCGGGCTTTAAAGTGGCGATTGTGGGTGCTGCTGGAGGCATTGGCCAACCTCTTGCAATGCTCATGAAGATGAACCCACTGGTTTCAGTTCTTCATCTCTATGATGTCGTTAATGCCCCCGGTGTCACAGCTGACATTAGCCACATGGATACCGGAGCTGTGGTGCGCGGTTTCTTGGGGCAGCCACAGCTTGAGAGCGCTCTCACGGGCATAGACCTTGTGATCATACCTGCCGGCGTTCCAAGGAAGCCTGGAATGACAAGGGATGATCTCTTCAAAATAAACGCGGGAATTGTCAGGACCATCTGTGAAGGAATTGCGAAAGCCTGTCCTAAGGCAATCGTCAACTTGATCAGTAATCCAGTGAACTCGACAGTTCCTATTGCAGCAGAGGTTTTCAAGAAAGCCGGGACTTATGACCCAAAGCGTCTTCTAGGAGTCACGATGCTTGATGTCGTGAGAGCAAATACTTTTGTTGCGGAAGTTCTTGGACTTGATCCTAGGGAAGTCGATGTTCCAGTTGTTGGTGGTCATGCTGGAGTGACCATTTTGCCGCTTCTGTCACAGGTCAAGCCTCCTTGCTCCTTCACCAAAGAAGAAACAGAATACCTAACAAACCGGATTCAAAACGGTGGAACAGAAGTTGTTGAGGCAAAAGCTGGGGCTGGTTCGGCAACACTGTCAATGGCATATGCAGCTGTGAAATTTGCGGATGCATGCCTCCGCGGCTTGAGAGGAGATGCTGGCGTTGTTGAATGCGCTTTTGTTGCCTCTGAGGTAACCGAACTTCCTTTCTTCGCAACCAAGGTCCGGCTTGGTCGTGGCGGAGCAGAGGAAATCTACCAACTCGGGCCTCTGAATGAGTATGAGAGGGTCGGATTAGAGAAGGCGAAAACGGAGTTAGCAGCAAGCATCCAGAAGGGGGTTTCGTTCATCAAGAAGTGA

Protein Analysis

357

Amino Acids

37.16

Weight (kDa)

8.14

Isoelectric Point (pI)

25.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 46 - 188 1e-46 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 190 - 353 3.8e-45 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09660 AT5G09660 AT5G09660 AT5G09660
fragaria_vesca FvH4_2g02490 FvH4_2g02490
malus_domestica MD05G1112700.v1.1
prunus_persica Prupe.8G157300_v2.0.a1
pyrus_communis pycom05g10810 pycom10g09980
rosa_chinensis RchiOBHm_Chr6g0246321
rosa_laevigata RLG00000015263
rosa_multiflora Rmu_sc0001066.1_g000009
rosa_roxburghii Rroxscaffold_7G00214680
rosa_rugosa Rorug05G0520300.1
rosa_samantha Rh6AG034500 Rh6BG029600 Rh6CG030000 Rh6DG028900
rosa_wichuraiana Rw6G002790 Rw6G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 1 cut(s) 650
AccII CGCG 3 cut(s) 294, 413, 852
AciI CCGC 9 cut(s) 120, 294, 413, 605, 680, 836, 850, 852, 951
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 2 cut(s) 64, 830
AcyI GRCGYC 1 cut(s) 125
AfeI AGCGCT 1 cut(s) 325
AfiI CCNNNNNNNGG 5 cut(s) 76, 120, 650, 936, 1048
AgsI TTSAA 4 cut(s) 403, 526, 752, 881
AjnI CCWGG 2 cut(s) 69, 376
AluBI AGCT 7 cut(s) 20, 47, 257, 284, 316, 785, 824
AluI AGCT 7 cut(s) 20, 47, 257, 284, 316, 785, 824
AlwI GGATC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 974
Aor51HI AGCGCT 1 cut(s) 325
AoxI GGCC 2 cut(s) 169, 977
ApeKI GCWGC 5 cut(s) 155, 307, 512, 821, 1031
ApoI RAATTY 2 cut(s) 64, 830
AspA2I CCTAGG 1 cut(s) 626
AspLEI GCGC 4 cut(s) 127, 294, 326, 887
AspS9I GGNCC 3 cut(s) 426, 934, 977
AsuC2I CCSGG 3 cut(s) 129, 246, 535
AsuHPI GGTGA 2 cut(s) 113, 706
AvaI CYCGRG 1 cut(s) 974
AvaII GGWCC 2 cut(s) 426, 934
AvrII CCTAGG 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 459
BalI TGGCCA 1 cut(s) 171
BanI GGYRCC 1 cut(s) 124
BarI GAAGNNNNNNTAC 2 cut(s) 897, 929
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 5 cut(s) 142, 319, 524, 833, 1043
BccI CCATC 2 cut(s) 74, 437
BciT130I CCWGG 2 cut(s) 71, 378
BciVI GTATCC 1 cut(s) 268
BclI TGATCA 2 cut(s) 348, 477
BcnI CCSGG 3 cut(s) 129, 246, 535
BfaI CTAG 2 cut(s) 560, 627
BfoI RGCGCY 2 cut(s) 128, 327
BfuAI ACCTGC 1 cut(s) 364
BfuI GTATCC 1 cut(s) 268
BglI GCCNNNNNGGC 1 cut(s) 852
BisI GCNGC 7 cut(s) 156, 308, 513, 680, 822, 853, 1032
BlnI CCTAGG 1 cut(s) 626
BlsI GCNGC 7 cut(s) 157, 309, 514, 681, 823, 854, 1033
Bme1390I CCNGG 5 cut(s) 71, 129, 246, 378, 535
Bme18I GGWCC 2 cut(s) 426, 934
BmeT110I CYCGRG 1 cut(s) 974
BmgT120I GGNCC 3 cut(s) 426, 934, 977
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 5 cut(s) 71, 129, 246, 378, 535
BmsI GCATC 4 cut(s) 561, 829, 856, 1047
BpiI GAAGAC 1 cut(s) 548
BpmI CTGGAG 2 cut(s) 180, 684
BpuEI CTTGAG 2 cut(s) 338, 877
BpuMI CCSGG 3 cut(s) 129, 246, 535
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 7 cut(s) 69, 114, 244, 368, 626, 850, 930
BsaWI WCCGGW 2 cut(s) 278, 744
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 120, 650, 936, 1048
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 210, 488, 644
Bse21I CCTNAGG 1 cut(s) 459
Bse3DI GCAATG 2 cut(s) 30, 189
BseBI CCWGG 2 cut(s) 71, 378
BseDI CCNNGG 7 cut(s) 69, 114, 244, 368, 626, 850, 930
BseGI GGATG 4 cut(s) 85, 397, 844, 1038
BseLI CCNNNNNNNGG 5 cut(s) 76, 120, 650, 936, 1048
BseMI GCAATG 2 cut(s) 30, 189
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 3 cut(s) 210, 488, 644
BseRI GAGGAG 1 cut(s) 876
BseXI GCAGC 5 cut(s) 142, 319, 524, 833, 1043
BseYI CCCAGC 1 cut(s) 785
Bsh1236I CGCG 3 cut(s) 294, 413, 852
BshFI GGCC 2 cut(s) 171, 979
BshNI GGYRCC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 974
BsiSI CCGG 7 cut(s) 128, 246, 279, 360, 534, 745, 937
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 5 cut(s) 76, 120, 650, 936, 1048
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 1 cut(s) 887
BsnI GGCC 2 cut(s) 171, 979
BsoBI CYCGRG 1 cut(s) 974
Bsp143I GATC 4 cut(s) 348, 394, 477, 622
BspACI CCGC 9 cut(s) 120, 294, 413, 605, 680, 836, 850, 852, 951
BspANI GGCC 2 cut(s) 171, 979
BspCNI CTCAG 1 cut(s) 892
BspFNI CGCG 3 cut(s) 294, 413, 852
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 30, 189
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 210, 488, 644
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 7 cut(s) 69, 114, 244, 368, 626, 850, 930
BssMI GATC 4 cut(s) 348, 394, 477, 622
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 4 cut(s) 114, 368, 626, 930
Bst2UI CCWGG 2 cut(s) 71, 378
Bst4CI ACNGT 3 cut(s) 502, 758, 807
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 125
BstC8I GCNNGC 4 cut(s) 361, 844, 871, 1036
BstDEI CTNAG 2 cut(s) 459, 900
BstDSI CCRYGG 1 cut(s) 850
BstEII GGTNACC 1 cut(s) 904
BstF5I GGATG 4 cut(s) 85, 397, 844, 1038
BstFNI CGCG 3 cut(s) 294, 413, 852
BstH2I RGCGCY 2 cut(s) 128, 327
BstHHI GCGC 4 cut(s) 127, 294, 326, 887
BstKTI GATC 4 cut(s) 351, 397, 480, 625
BstMBI GATC 4 cut(s) 348, 394, 477, 622
BstMWI GCNNNNNNNGC 5 cut(s) 313, 797, 821, 852, 893
BstNI CCWGG 2 cut(s) 71, 378
BstNSI RCATGY 1 cut(s) 846
BstPI GGTNACC 1 cut(s) 904
BstSCI CCNGG 5 cut(s) 69, 127, 244, 376, 533
BstUI CGCG 3 cut(s) 294, 413, 852
BstV1I GCAGC 5 cut(s) 142, 319, 524, 833, 1043
BstV2I GAAGAC 1 cut(s) 548
Bsu36I CCTNAGG 1 cut(s) 459
BsuI GTATCC 1 cut(s) 268
BsuRI GGCC 2 cut(s) 171, 979
BtgI CCRYGG 1 cut(s) 850
BtsCI GGATG 4 cut(s) 85, 397, 844, 1038
BtsIMutI CAGTG 3 cut(s) 203, 495, 803
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 4 cut(s) 361, 844, 871, 1036
CciI TCATGA 1 cut(s) 189
CfoI GCGC 4 cut(s) 127, 294, 326, 887
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 3 cut(s) 426, 934, 977
Cfr42I CCGCGG 1 cut(s) 853
CseI GACGC 2 cut(s) 36, 542
CviAII CATG 4 cut(s) 190, 271, 659, 843
DdeI CTNAG 2 cut(s) 459, 900
DinI GGCGCC 1 cut(s) 126
DpnI GATC 4 cut(s) 350, 396, 479, 624
DpnII GATC 4 cut(s) 348, 394, 477, 622
DraI TTTAAA 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EciI GGCGGA 1 cut(s) 966
Eco130I CCWWGG 4 cut(s) 114, 368, 626, 930
Eco47I GGWCC 2 cut(s) 426, 934
Eco47III AGCGCT 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 974
Eco91I GGTNACC 1 cut(s) 904
EcoO65I GGTNACC 1 cut(s) 904
EcoRII CCWGG 2 cut(s) 69, 376
EcoT14I CCWWGG 4 cut(s) 114, 368, 626, 930
EcoT22I ATGCAT 1 cut(s) 844
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 4 cut(s) 114, 368, 626, 930
FaeI CATG 4 cut(s) 193, 274, 662, 846
FaqI GGGAC 1 cut(s) 550
FatI CATG 4 cut(s) 189, 270, 658, 842
FauI CCCGC 2 cut(s) 113, 406
FauNDI CATATG 1 cut(s) 817
FbaI TGATCA 2 cut(s) 348, 477
Fnu4HI GCNGC 7 cut(s) 156, 308, 513, 680, 822, 853, 1032
FokI GGATG 4 cut(s) 92, 404, 851, 1025
Fsp4HI GCNGC 7 cut(s) 156, 308, 513, 680, 822, 853, 1032
FspBI CTAG 2 cut(s) 560, 627
GlaI GCGC 4 cut(s) 126, 293, 325, 886
GluI GCNGC 7 cut(s) 156, 308, 513, 680, 822, 853, 1032
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 2 cut(s) 180, 684
HaeII RGCGCY 2 cut(s) 128, 327
HaeIII GGCC 2 cut(s) 171, 979
HapII CCGG 7 cut(s) 128, 246, 279, 360, 534, 745, 937
HgaI GACGC 2 cut(s) 36, 542
HhaI GCGC 4 cut(s) 127, 294, 326, 887
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 4 cut(s) 193, 274, 662, 846
Hin6I GCGC 4 cut(s) 125, 292, 324, 885
HinP1I GCGC 4 cut(s) 125, 292, 324, 885
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HinfI GANTC 3 cut(s) 5, 564, 748
HpaII CCGG 7 cut(s) 128, 246, 279, 360, 534, 745, 937
HphI GGTGA 2 cut(s) 113, 706
Hpy166II GTNNAC 2 cut(s) 472, 493
Hpy188I TCNGA 3 cut(s) 901, 984, 1004
Hpy188III TCNNGA 7 cut(s) 190, 424, 526, 568, 583, 1042, 1063
Hpy8I GTNNAC 2 cut(s) 472, 493
HpyAV CCTTC 4 cut(s) 431, 721, 1006, 1039
HpyCH4III ACNGT 3 cut(s) 502, 758, 807
HpyCH4V TGCA 6 cut(s) 35, 108, 182, 512, 821, 842
HpyF10VI GCNNNNNNNGC 5 cut(s) 313, 797, 821, 852, 893
HpyF3I CTNAG 2 cut(s) 459, 900
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 4 cut(s) 193, 274, 662, 846
HspAI GCGC 4 cut(s) 125, 292, 324, 885
KasI GGCGCC 1 cut(s) 124
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Ksp22I TGATCA 2 cut(s) 348, 477
KspI CCGCGG 1 cut(s) 853
Kzo9I GATC 4 cut(s) 348, 394, 477, 622
LmnI GCTCC 4 cut(s) 17, 281, 713, 953
Lsp1109I GCAGC 5 cut(s) 142, 319, 524, 833, 1043
LweI GCATC 4 cut(s) 561, 829, 856, 1047
MaeI CTAG 2 cut(s) 560, 627
MaeIII GTNAC 5 cut(s) 250, 565, 667, 687, 904
MalI GATC 4 cut(s) 350, 396, 479, 624
MboI GATC 4 cut(s) 348, 394, 477, 622
MboII GAAGA 6 cut(s) 205, 209, 391, 548, 734, 913
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 4 cut(s) 64, 417, 441, 830
MluNI TGGCCA 1 cut(s) 171
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 2 cut(s) 14, 573
MmeI TCCRAC 1 cut(s) 982
Mox20I TGGCCA 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 844
MroNI GCCGGC 1 cut(s) 359
MscI TGGCCA 1 cut(s) 171
MseI TTAA 2 cut(s) 135, 237
MslI CAYNNNNRTG 2 cut(s) 194, 225
Msp20I TGGCCA 1 cut(s) 171
MspA1I CMGCKG 3 cut(s) 257, 824, 852
MspI CCGG 7 cut(s) 128, 246, 279, 360, 534, 745, 937
MspR9I CCNGG 5 cut(s) 71, 129, 246, 378, 535
Mva1269I GAATGC 1 cut(s) 887
MvaI CCWGG 2 cut(s) 71, 378
MvnI CGCG 3 cut(s) 294, 413, 852
MwoI GCNNNNNNNGC 5 cut(s) 313, 797, 821, 852, 893
NaeI GCCGGC 1 cut(s) 361
NarI GGCGCC 1 cut(s) 125
NciI CCSGG 3 cut(s) 129, 246, 535
NdeI CATATG 1 cut(s) 817
NdeII GATC 4 cut(s) 348, 394, 477, 622
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 4 cut(s) 193, 274, 662, 846
NlaIV GGNNCC 1 cut(s) 126
NmuCI GTSAC 4 cut(s) 250, 565, 667, 687
NsiI ATGCAT 1 cut(s) 844
NspI RCATGY 1 cut(s) 846
PaeI GCATGC 1 cut(s) 846
PagI TCATGA 1 cut(s) 189
PctI GAATGC 1 cut(s) 887
PdiI GCCGGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 748
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 7 cut(s) 157, 309, 514, 681, 823, 854, 1033
PleI GAGTC 2 cut(s) 13, 572
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 2 cut(s) 13, 572
Psp6I CCWGG 2 cut(s) 69, 376
PspEI GGTNACC 1 cut(s) 904
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 2 cut(s) 69, 376
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 3 cut(s) 426, 934, 977
PvuII CAGCTG 2 cut(s) 257, 824
RseI CAYNNNNRTG 2 cut(s) 194, 225
SacII CCGCGG 1 cut(s) 853
SaqAI TTAA 2 cut(s) 135, 237
SatI GCNGC 7 cut(s) 156, 308, 513, 680, 822, 853, 1032
Sau3AI GATC 4 cut(s) 348, 394, 477, 622
Sau96I GGNCC 3 cut(s) 426, 934, 977
SchI GAGTC 2 cut(s) 14, 573
ScrFI CCNGG 5 cut(s) 71, 129, 246, 378, 535
SfaNI GCATC 4 cut(s) 561, 829, 856, 1047
SfoI GGCGCC 1 cut(s) 126
Sfr303I CCGCGG 1 cut(s) 853
SgrBI CCGCGG 1 cut(s) 853
SinI GGWCC 2 cut(s) 426, 934
SmiMI CAYNNNNRTG 2 cut(s) 194, 225
SmlI CTYRAG 2 cut(s) 317, 856
SmoI CTYRAG 2 cut(s) 317, 856
SphI GCATGC 1 cut(s) 846
Sse9I AATT 4 cut(s) 64, 417, 441, 830
SsiI CCGC 9 cut(s) 120, 294, 413, 605, 680, 836, 850, 852, 951
SspDI GGCGCC 1 cut(s) 124
SspMI CTAG 2 cut(s) 560, 627
StyD4I CCNGG 5 cut(s) 69, 127, 244, 376, 533
StyI CCWWGG 4 cut(s) 114, 368, 626, 930
TaaI ACNGT 3 cut(s) 502, 758, 807
TaqI TCGA 3 cut(s) 8, 497, 636
TasI AATT 4 cut(s) 64, 417, 441, 830
TauI GCSGC 2 cut(s) 682, 855
TfiI GAWTC 1 cut(s) 748
Tru1I TTAA 2 cut(s) 135, 237
Tru9I TTAA 2 cut(s) 135, 237
TscAI CASTG 3 cut(s) 210, 495, 810
TseFI GTSAC 4 cut(s) 250, 565, 667, 687
TseI GCWGC 5 cut(s) 155, 307, 512, 821, 1031
Tsp45I GTSAC 4 cut(s) 250, 565, 667, 687
TspDTI ATGAA 4 cut(s) 206, 209, 212, 1048
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 3 cut(s) 210, 495, 810
Van91I CCANNNNNTGG 1 cut(s) 650
VpaK11BI GGWCC 2 cut(s) 426, 934
XapI RAATTY 2 cut(s) 64, 830
XceI RCATGY 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XmaJI CCTAGG 1 cut(s) 626
XspI CTAG 2 cut(s) 560, 627
Zsp2I ATGCAT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.