RLG00000015263

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009925
Physical Location & Seq
Forward (+)
63784483 .. 63786827
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015263

Sequence Viewer

Length: 1044 bp
ATGGCGTCCAGCGTAGAAGTGAATCAACGCATTGCAAGGCTCTCAGCTCATCTCTACCCTTCAAATCTCCAGATGGAGGACAGCAGTTCTGCTGTGAGAAGAGCTGATTGCAGAGCCAAAGGAGGAGCACCTGGTTTTAAAGTGGCTATTTTGGGTGCTGCTGGAGGCATTGGACAACCTCTTGCAATGCTCATGAAAATGAATCCACTGGTCTCAGTTCTTCATCTCTATGATGTCGTCAATGCCCCTGGTGTGACAGCTGATATTAGTCACATGGACACTGGCGCTGTGGTGCGCGGGTTCTTGGGGCAGGCACAGCTTGACAATGCTCTCACAGGTATTGACCTTGTGATCATACCTGCCGGTGTTCCAAGGAAGCCTGGAATGACCAGGGATGATCTTTTCAAGATCAATGCCGGAATAGTTAGGACTCTTTGCGAGGGAATTGCAAGGTGCTGCCCTAGAGCAATTGTCAACTTGATCAGTAATCCAGTGAACTCTACAGTTCCTATAGCAGCTGAGGTTTTCAAGAAAGCTGGTACTTACGACCCAAAGCGACTGCTAGGGGTTACAATGCTTGATGTCGTGAGAGCAAACACATTTGTTGCTGAAGTTCTGGGACTTGATCCTAGAGAAGTTGATGTTCCTGTTGTTGGAGGCCATTCAGGAGTGACCATTTTGCCCCTTCTGTCGCAGGTCAAGCCTCCTTGCTCCTTCACTCGAGAAGAAACAGAATACCTGACTGATCGTATTCAAAATGGTGGAACAGAAGTTGTTGAGGCAAAGGCTGGGGCTGGCTCGGCAACACTATCAATGGCATATGCAGCTGTCAAGTTTGCGGATGCATGCCTTCGTGGCTTGAGAGGAGATGCTGGTGTTGTGGAATGCTCTTTTGTTGCATCTCAGGTACGGCTTGGCCGTAATGGAGCTGAGGAAGTCTACCAACTTGGGCCTCTGAATGAGTATGAGAGGGTCGGTTTAGAGAAGGCGAAAAAAGAGTTAGCAGGAAGCATCGAAAAGGGGATTTCTTTCATCAGGAAGTAA

Protein Analysis

348

Amino Acids

36.67

Weight (kDa)

8.42

Isoelectric Point (pI)

26.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 47 - 189 9.7e-46 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 191 - 344 1.6e-40 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09660 AT5G09660 AT5G09660 AT5G09660
fragaria_vesca FvH4_2g02490 FvH4_2g02490
malus_domestica MD05G1112700.v1.1
prunus_persica Prupe.8G157300_v2.0.a1
pyrus_communis pycom05g10810 pycom10g09980
rosa_chinensis RchiOBHm_Chr6g0246321
rosa_laevigata RLG00000015263
rosa_multiflora Rmu_sc0001066.1_g000009
rosa_roxburghii Rroxscaffold_7G00214680
rosa_rugosa Rorug05G0520300.1
rosa_samantha Rh6AG034500 Rh6BG029600 Rh6CG030000 Rh6DG028900
rosa_wichuraiana Rw6G002790 Rw6G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 367, 685
AccI GTMKAC 1 cut(s) 939
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 297, 839
AclWI GGATC 1 cut(s) 620
AcoI YGGCCR 1 cut(s) 916
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 541, 909
AfiI CCNNNNNNNGG 2 cut(s) 76, 653
AgsI TTSAA 4 cut(s) 63, 406, 529, 755
AjnI CCWGG 4 cut(s) 130, 247, 379, 389
AluBI AGCT 8 cut(s) 47, 104, 260, 319, 518, 536, 827, 929
AluI AGCT 8 cut(s) 47, 104, 260, 319, 518, 536, 827, 929
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 720
AoxI GGCC 3 cut(s) 658, 916, 950
ApeKI GCWGC 4 cut(s) 158, 456, 515, 824
AspLEI GCGC 2 cut(s) 287, 297
AspS9I GGNCC 1 cut(s) 950
AvaI CYCGRG 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 130
BbvCI CCTCAGC 2 cut(s) 519, 930
BbvI GCAGC 4 cut(s) 145, 443, 527, 836
BccI CCATC 1 cut(s) 67
BceAI ACGGC 2 cut(s) 903, 926
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 4 cut(s) 132, 249, 381, 391
BclI TGATCA 2 cut(s) 351, 480
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 3 cut(s) 462, 563, 630
BfmI CTRYAG 2 cut(s) 501, 510
BfoI RGCGCY 1 cut(s) 288
BfuAI ACCTGC 2 cut(s) 367, 685
BglI GCCNNNNNGGC 1 cut(s) 855
BisI GCNGC 4 cut(s) 159, 457, 516, 825
BlsI GCNGC 4 cut(s) 160, 458, 517, 826
Bme1390I CCNGG 4 cut(s) 132, 249, 381, 391
BmeT110I CYCGRG 1 cut(s) 720
BmgT120I GGNCC 1 cut(s) 950
BmrFI CCNGG 4 cut(s) 132, 249, 381, 391
BmsI GCATC 4 cut(s) 832, 859, 908, 1020
BpmI CTGGAG 2 cut(s) 53, 183
Bpu10I CCTNAGC 2 cut(s) 519, 930
BpuEI CTTGAG 1 cut(s) 880
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 247, 371, 390
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 653
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 3 cut(s) 213, 286, 491
Bse3DI GCAATG 2 cut(s) 30, 192
BseBI CCWGG 4 cut(s) 132, 249, 381, 391
BseDI CCNNGG 3 cut(s) 247, 371, 390
BseGI GGATG 2 cut(s) 400, 847
BseLI CCNNNNNNNGG 2 cut(s) 76, 653
BseMI GCAATG 2 cut(s) 30, 192
BseMII CTCAG 5 cut(s) 57, 228, 510, 917, 921
BseNI ACTGG 3 cut(s) 213, 286, 491
BseRI GAGGAG 2 cut(s) 138, 879
BseXI GCAGC 4 cut(s) 145, 443, 527, 836
BseYI CCCAGC 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 660, 918, 952
BsiHKAI GWGCWC 1 cut(s) 130
BsiHKCI CYCGRG 1 cut(s) 720
BsiSI CCGG 2 cut(s) 363, 417
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 2 cut(s) 76, 653
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 3 cut(s) 660, 918, 952
Bso31I GGTCTC 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 720
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 6 cut(s) 351, 397, 408, 480, 625, 745
BspACI CCGC 2 cut(s) 297, 839
BspANI GGCC 3 cut(s) 660, 918, 952
BspCNI CTCAG 5 cut(s) 56, 227, 511, 916, 922
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 192
BspMI ACCTGC 2 cut(s) 367, 685
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 94
BspTNI GGTCTC 1 cut(s) 217
BsrDI GCAATG 2 cut(s) 30, 192
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 3 cut(s) 213, 286, 491
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 247, 371, 390
BssMI GATC 6 cut(s) 351, 397, 408, 480, 625, 745
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 371
Bst2UI CCWGG 4 cut(s) 132, 249, 381, 391
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 312, 796, 847
BstDEI CTNAG 5 cut(s) 43, 214, 519, 903, 930
BstF5I GGATG 2 cut(s) 400, 847
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 287, 297
BstKTI GATC 6 cut(s) 354, 400, 411, 483, 628, 748
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 6 cut(s) 351, 397, 408, 480, 625, 745
BstMWI GCNNNNNNNGC 5 cut(s) 316, 700, 800, 824, 855
BstNI CCWGG 4 cut(s) 132, 249, 381, 391
BstNSI RCATGY 1 cut(s) 849
BstSCI CCNGG 4 cut(s) 130, 247, 379, 389
BstSFI CTRYAG 2 cut(s) 501, 510
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 4 cut(s) 145, 443, 527, 836
BsuRI GGCC 3 cut(s) 660, 918, 952
BtsCI GGATG 2 cut(s) 400, 847
BtsIMutI CAGTG 3 cut(s) 206, 279, 498
BveI ACCTGC 2 cut(s) 367, 685
Cac8I GCNNGC 3 cut(s) 312, 796, 847
CciI TCATGA 1 cut(s) 192
CfoI GCGC 2 cut(s) 287, 297
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 950
CsiI ACCWGGT 1 cut(s) 130
Csp6I GTAC 2 cut(s) 540, 908
CviAII CATG 3 cut(s) 193, 274, 846
CviQI GTAC 2 cut(s) 540, 908
DdeI CTNAG 5 cut(s) 43, 214, 519, 903, 930
DpnI GATC 6 cut(s) 353, 399, 410, 482, 627, 747
DpnII GATC 6 cut(s) 351, 397, 408, 480, 625, 745
DraI TTTAAA 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 916
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 630
Eco88I CYCGRG 1 cut(s) 720
EcoRII CCWGG 4 cut(s) 130, 247, 379, 389
EcoT14I CCWWGG 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 847
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 3 cut(s) 196, 277, 849
FaiI YATR 9 cut(s) 194, 231, 275, 356, 512, 820, 822, 847, 966
FaqI GGGAC 1 cut(s) 633
FatI CATG 3 cut(s) 192, 273, 845
FauI CCCGC 1 cut(s) 290
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 2 cut(s) 351, 480
FblI GTMKAC 1 cut(s) 939
Fnu4HI GCNGC 4 cut(s) 159, 457, 516, 825
FokI GGATG 2 cut(s) 407, 854
Fsp4HI GCNGC 4 cut(s) 159, 457, 516, 825
FspBI CTAG 3 cut(s) 462, 563, 630
GlaI GCGC 2 cut(s) 286, 296
GluI GCNGC 4 cut(s) 159, 457, 516, 825
GsaI CCCAGC 1 cut(s) 792
GsuI CTGGAG 2 cut(s) 53, 183
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 3 cut(s) 660, 918, 952
HapII CCGG 2 cut(s) 363, 417
HhaI GCGC 2 cut(s) 287, 297
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 196, 277, 849
Hin6I GCGC 2 cut(s) 285, 295
HinP1I GCGC 2 cut(s) 285, 295
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 3 cut(s) 22, 202, 430
HpaII CCGG 2 cut(s) 363, 417
Hpy166II GTNNAC 3 cut(s) 475, 496, 940
Hpy188I TCNGA 1 cut(s) 957
Hpy188III TCNNGA 8 cut(s) 70, 193, 406, 529, 586, 666, 722, 1036
Hpy8I GTNNAC 3 cut(s) 475, 496, 940
HpyAV CCTTC 5 cut(s) 69, 695, 724, 860, 979
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4V TGCA 7 cut(s) 35, 111, 185, 449, 824, 845, 899
HpyF10VI GCNNNNNNNGC 5 cut(s) 316, 700, 800, 824, 855
HpyF3I CTNAG 5 cut(s) 43, 214, 519, 903, 930
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 196, 277, 849
HspAI GCGC 2 cut(s) 285, 295
Ksp22I TGATCA 2 cut(s) 351, 480
Kzo9I GATC 6 cut(s) 351, 397, 408, 480, 625, 745
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 3 cut(s) 125, 716, 926
Lsp1109I GCAGC 4 cut(s) 145, 443, 527, 836
LweI GCATC 4 cut(s) 832, 859, 908, 1020
MabI ACCWGGT 1 cut(s) 130
MaeI CTAG 3 cut(s) 462, 563, 630
MaeIII GTNAC 4 cut(s) 253, 269, 568, 670
MalI GATC 6 cut(s) 353, 399, 410, 482, 627, 747
MboI GATC 6 cut(s) 351, 397, 408, 480, 625, 745
MboII GAAGA 3 cut(s) 111, 212, 737
MfeI CAATTG 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 2 cut(s) 444, 468
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 634
Mph1103I ATGCAT 1 cut(s) 847
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 2 cut(s) 197, 228
MspA1I CMGCKG 3 cut(s) 260, 518, 827
MspI CCGG 2 cut(s) 363, 417
MspR9I CCNGG 4 cut(s) 132, 249, 381, 391
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 4 cut(s) 132, 249, 381, 391
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 5 cut(s) 316, 700, 800, 824, 855
NdeI CATATG 1 cut(s) 820
NdeII GATC 6 cut(s) 351, 397, 408, 480, 625, 745
NlaIII CATG 3 cut(s) 196, 277, 849
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 3 cut(s) 253, 269, 670
NsiI ATGCAT 1 cut(s) 847
NspI RCATGY 1 cut(s) 849
PaeI GCATGC 1 cut(s) 849
PaeR7I CTCGAG 1 cut(s) 720
PagI TCATGA 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 916
PctI GAATGC 1 cut(s) 890
PfeI GAWTC 2 cut(s) 22, 202
PkrI GCNGC 4 cut(s) 160, 458, 517, 826
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 4 cut(s) 130, 247, 379, 389
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 4 cut(s) 130, 247, 379, 389
PspPI GGNCC 1 cut(s) 950
PvuII CAGCTG 3 cut(s) 260, 518, 827
RsaI GTAC 2 cut(s) 541, 909
RsaNI GTAC 2 cut(s) 540, 908
RseI CAYNNNNRTG 2 cut(s) 197, 228
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 4 cut(s) 159, 457, 516, 825
Sau3AI GATC 6 cut(s) 351, 397, 408, 480, 625, 745
Sau96I GGNCC 1 cut(s) 950
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 4 cut(s) 132, 249, 381, 391
SduI GDGCHC 1 cut(s) 130
SexAI ACCWGGT 1 cut(s) 130
SfaNI GCATC 4 cut(s) 832, 859, 908, 1020
SfcI CTRYAG 2 cut(s) 501, 510
Sfr274I CTCGAG 1 cut(s) 720
SlaI CTCGAG 1 cut(s) 720
SmiMI CAYNNNNRTG 2 cut(s) 197, 228
SmlI CTYRAG 2 cut(s) 720, 859
SmoI CTYRAG 2 cut(s) 720, 859
SphI GCATGC 1 cut(s) 849
Sse9I AATT 2 cut(s) 444, 468
SsiI CCGC 2 cut(s) 297, 839
SspMI CTAG 3 cut(s) 462, 563, 630
StyD4I CCNGG 4 cut(s) 130, 247, 379, 389
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 1 cut(s) 505
TaqI TCGA 2 cut(s) 721, 1014
TasI AATT 2 cut(s) 444, 468
TfiI GAWTC 2 cut(s) 22, 202
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 3 cut(s) 213, 286, 498
TseFI GTSAC 3 cut(s) 253, 269, 670
TseI GCWGC 4 cut(s) 158, 456, 515, 824
Tsp45I GTSAC 3 cut(s) 253, 269, 670
TspDTI ATGAA 4 cut(s) 209, 212, 215, 1021
TspRI CASTG 3 cut(s) 213, 286, 498
XceI RCATGY 1 cut(s) 849
XhoI CTCGAG 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 939
XspI CTAG 3 cut(s) 462, 563, 630
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.