AT5G12900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014307
Physical Location & Seq
Reverse (-)
4071896 .. 4074619
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12900.1

Sequence Viewer

Length: 1689 bp
ATGGCTTCTTCTGAAACAAGAATGAACAAGGCAAGAGAAAAAGAAGAAGCCATAGCAAATGGAGTGAAGTTGAGAGCACTTCAAGCGAGTCTTATGCAGATGAAGTCAAGCCCATCTTCCAATTACAGTCTCAGAAACCCTTCTTCCTCTTCTGCTGCTTCACCTGCTTCTCGTCCTCTCCCTAACCTCTCTGCTCATGATTACCCTGTTTTCACTCCTAGCTATGAAGATGAGCCTGTGTCTGCATTTCATCACAAGAATCTCACTTTATCTGAGACTTGGGATGAAGATGGTGTTGGTTTAGTAGATGGAGATACTTATCTCTCTGATTCTTACAAAACCTCAACTTCAAGAAAGACTGTGATGATGCCTCATCAAGATTCTCATCATCATGTTTACACTATGTCGGACGCTCTCAGATCTCCACCGTTACATTTTTATACAACCGGTAGAAGCAACTGTGGCTCGGTCGACTTTAGATCGGTCTCTTCTTGCAATGACTACAATAAACAAAAAGGTTTCGACACGAAGAGCTTGAAGAGCTCTAATCTTGTTGTGCCCTTGACGGATTCGCACTCTGCTGTTGTTTCTTCTCAGCCAAGAAACCGTGGAGGTAGAGTAATGTCTTGGTTATTCCCTAAGTTAAAGAAGAAACAAAAGAGTAATTCGATCTTTAATTCACCTAGTATAACTGAGAAGTCAGAGGAAGTTTCTGAGGTGTTGAAGGATTCTGGTTCTGGTGTTGAGAAGTTGAAGAGAGAATTGATGGAAGCAAACAGAAGTAGGGATGCTGCGTTAACACAAGTCTCAGAGATGAAATCTTCATTGGGAGAGTTAAGTGAGAAGCTTCAGTACTTGGAAAGTTACTGTGACAATTTAAAGAAAGCTTTGAGAGAAGCAACAGAAGTAGTGTCACAGGAGAATAGTGGTGGAAGAAGCTCAGGGAAAAAGAACTCAGAGATGCCAGTGAGTGAAGAAGTGATGGTTGAAGGGTTCTTGCAGATTGTATCAGAAGCAAGATTATCGATAAAGCAGTTCTTGAAAACATTAGTTTCAGAGATTGATGAAGAAGACTCGACTCTAATTGGTAACATCAACACTCTCCTTCAACCTCACAACTTATCATTCACTTCTAAGTACTCCAAGATCATTCAGTACCACTTAGAAGCAATCATAAGCCAATCAGTATACCAAGACTTTGAGAACTGCGTATTCCAGAAGAACGGTAAGCCGAAACTGCTTGATCCGGAACAAGATCGACAAGCGAACTTCTCGTCCTTTGCTTCCTTGAGAAACTTGAGCTGGAACGAGGTATTGAAAAAGGGTACAAAATACTACAGTGATGAGTTCAGTAGATTCTGCGATGAGAAGATGAGTTTGATCATTACAACATTGAATTGGACAAGACCTTGGTCAGAACAAATGCTTCAAGCATTCTTTGTGGCTGCAAAATGTGTATGGTTGCTTCATTTGCTTGCCTTCTCGTTCAATCCAGCGTTAGGGATATTGAGAGTTGAGGAGAACAGAGAGTTTGAATCAAGTTTTATGGAAGATATGGGTGCAGATAGGCAGAGATCAGCATTGTCCCGTGGACCGGCACGGGTTAAGGTTATGGTAATGCCTGGGTTTTATGTTTTAGATAGGGTTTTGAGATGTAAGGTTCTTTGCAGGTACAAGTCATTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

562

Amino Acids

63.33

Weight (kDa)

8.41

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIL1_IRKI_C PF24994 502 - 555 1.5e-06 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12900
fragaria_vesca FvH4_3g43680 FvH4_3g43680
malus_domestica MD11G1021700.v1.1
prunus_persica Prupe.6G016100_v2.0.a1
pyrus_communis pycom03g01740 pycom11g01760
rosa_chinensis RchiOBHm_Chr5g0078341
rosa_laevigata RLG00000036768
rosa_roxburghii Rroxscaffold_1G00003900
rosa_rugosa Rorug05G0460200
rosa_samantha Rh5AG514100 Rh5BG537000 Rh5CG560500 Rh5DG548600
rosa_wichuraiana Rw5G047730 Rw5G047870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 2 cut(s) 172, 1659
AccI GTMKAC 2 cut(s) 471, 1188
AccIII TCCGGA 1 cut(s) 1246
AclWI GGATC 1 cut(s) 1238
AcuI CTGAAG 1 cut(s) 833
AfaI GTAC 5 cut(s) 854, 1139, 1157, 1327, 1673
AfiI CCNNNNNNNGG 2 cut(s) 1499, 1594
AgeI ACCGGT 1 cut(s) 446
AjnI CCWGG 1 cut(s) 1621
AjuI GAANNNNNNNTTGG 4 cut(s) 279, 311, 809, 841
AloI GAACNNNNNNTCC 2 cut(s) 1259, 1291
AluBI AGCT 7 cut(s) 222, 534, 543, 847, 887, 939, 1302
AluI AGCT 7 cut(s) 222, 534, 543, 847, 887, 939, 1302
Alw21I GWGCWC 2 cut(s) 79, 545
Alw26I GTCTC 4 cut(s) 134, 269, 490, 811
AlwI GGATC 1 cut(s) 1238
Aor13HI TCCGGA 1 cut(s) 1246
ApeKI GCWGC 3 cut(s) 155, 791, 1445
ArsI GACNNNNNNTTYG 2 cut(s) 1259, 1291
AsiGI ACCGGT 1 cut(s) 446
Asp700I GAANNNNTTC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 1592
AsuHPI GGTGA 2 cut(s) 153, 672
AvaII GGWCC 1 cut(s) 1592
BaeGI GKGCMC 1 cut(s) 561
BanII GRGCYC 1 cut(s) 545
BarI GAAGNNNNNNTAC 4 cut(s) 836, 868, 1449, 1481
BbsI GAAGAC 1 cut(s) 1077
Bbv12I GWGCWC 2 cut(s) 79, 545
BbvI GCAGC 3 cut(s) 142, 778, 1432
BccI CCATC 5 cut(s) 121, 284, 302, 760, 976
BcgI CGANNNNNNTGC 4 cut(s) 76, 110, 1005, 1039
BciT130I CCWGG 1 cut(s) 1623
BclI TGATCA 1 cut(s) 1380
BcoDI GTCTC 4 cut(s) 134, 269, 490, 811
BfaI CTAG 2 cut(s) 219, 684
BfmI CTRYAG 1 cut(s) 1336
BfuAI ACCTGC 2 cut(s) 172, 1659
BglII AGATCT 1 cut(s) 419
BisI GCNGC 3 cut(s) 156, 792, 1446
BlsI GCNGC 3 cut(s) 157, 793, 1447
BmcAI AGTACT 2 cut(s) 854, 1139
Bme1390I CCNGG 1 cut(s) 1623
Bme18I GGWCC 1 cut(s) 1592
BmgT120I GGNCC 1 cut(s) 1592
BmrFI CCNGG 1 cut(s) 1623
BmsI GCATC 3 cut(s) 357, 778, 951
BoxI GACNNNNGTC 1 cut(s) 1411
BpiI GAAGAC 1 cut(s) 1077
Bpu10I CCTNAGC 1 cut(s) 940
BpuEI CTTGAG 2 cut(s) 1309, 1318
Bsa29I ATCGAT 1 cut(s) 1025
BsaBI GATNNNNATC 2 cut(s) 318, 384
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 4 cut(s) 607, 1409, 1588, 1622
BsaWI WCCGGW 2 cut(s) 446, 1246
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 1499, 1594
Bse118I RCCGGY 2 cut(s) 446, 1594
Bse1I ACTGG 1 cut(s) 965
Bse3DI GCAATG 1 cut(s) 502
Bse8I GATNNNNATC 2 cut(s) 318, 384
BseAI TCCGGA 1 cut(s) 1246
BseBI CCWGG 1 cut(s) 1623
BseCI ATCGAT 1 cut(s) 1025
BseDI CCNNGG 4 cut(s) 607, 1409, 1588, 1622
BseGI GGATG 2 cut(s) 289, 793
BseJI GATNNNNATC 2 cut(s) 318, 384
BseLI CCNNNNNNNGG 2 cut(s) 1499, 1594
BseMI GCAATG 1 cut(s) 502
BseMII CTCAG 9 cut(s) 145, 264, 430, 608, 684, 705, 822, 954, 969
BseNI ACTGG 1 cut(s) 965
BseRI GAGGAG 1 cut(s) 1532
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 3 cut(s) 142, 778, 1432
BsgI GTGCAG 1 cut(s) 1581
Bsh1285I CGRYCG 1 cut(s) 471
BshTI ACCGGT 1 cut(s) 446
BshVI ATCGAT 1 cut(s) 1025
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 79, 545
BsiSI CCGG 3 cut(s) 447, 1247, 1595
BslFI GGGAC 1 cut(s) 1570
BslI CCNNNNNNNGG 2 cut(s) 1499, 1594
BsmAI GTCTC 4 cut(s) 134, 269, 490, 811
BsmFI GGGAC 1 cut(s) 1570
BsmI GAATGC 1 cut(s) 1433
Bso31I GGTCTC 1 cut(s) 490
Bsp1286I GDGCHC 3 cut(s) 79, 545, 561
Bsp13I TCCGGA 1 cut(s) 1246
Bsp143I GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
BspCNI CTCAG 9 cut(s) 144, 265, 429, 607, 685, 706, 821, 953, 968
BspDI ATCGAT 1 cut(s) 1025
BspEI TCCGGA 1 cut(s) 1246
BspHI TCATGA 1 cut(s) 196
BspMI ACCTGC 2 cut(s) 172, 1659
BspPI GGATC 1 cut(s) 1238
BspQI GCTCTTC 2 cut(s) 524, 533
BspTNI GGTCTC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 502
BsrFI RCCGGY 2 cut(s) 446, 1594
BsrI ACTGG 1 cut(s) 965
BssAI RCCGGY 2 cut(s) 446, 1594
BssECI CCNNGG 4 cut(s) 607, 1409, 1588, 1622
BssMI GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
BssNAI GTATAC 1 cut(s) 1189
BssT1I CCWWGG 1 cut(s) 1409
Bst1107I GTATAC 1 cut(s) 1189
Bst2UI CCWGG 1 cut(s) 1623
Bst4CI ACNGT 8 cut(s) 128, 361, 429, 461, 608, 869, 1226, 1340
Bst6I CTCTTC 5 cut(s) 154, 493, 524, 533, 749
BstC8I GCNNGC 1 cut(s) 1476
BstDSI CCRYGG 2 cut(s) 607, 1588
BstF5I GGATG 2 cut(s) 289, 793
BstKTI GATC 8 cut(s) 422, 482, 672, 1149, 1246, 1258, 1383, 1577
BstMAI GTCTC 4 cut(s) 134, 269, 490, 811
BstMBI GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 6 cut(s) 83, 164, 462, 540, 1237, 1471
BstNI CCWGG 1 cut(s) 1623
BstPAI GACNNNNGTC 1 cut(s) 1411
BstSCI CCNGG 1 cut(s) 1621
BstSFI CTRYAG 1 cut(s) 1336
BstSLI GKGCMC 1 cut(s) 561
BstV1I GCAGC 3 cut(s) 142, 778, 1432
BstV2I GAAGAC 1 cut(s) 1077
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 1189
Bsu15I ATCGAT 1 cut(s) 1025
BsuTUI ATCGAT 1 cut(s) 1025
BtgI CCRYGG 2 cut(s) 607, 1588
BtgZI GCGATG 1 cut(s) 1377
BtsCI GGATG 2 cut(s) 289, 793
BtsIMutI CAGTG 2 cut(s) 972, 1345
BveI ACCTGC 2 cut(s) 172, 1659
Cac8I GCNNGC 1 cut(s) 1476
CciI TCATGA 1 cut(s) 196
Cfr10I RCCGGY 2 cut(s) 446, 1594
Cfr13I GGNCC 1 cut(s) 1592
ClaI ATCGAT 1 cut(s) 1025
CseI GACGC 1 cut(s) 419
Csp6I GTAC 5 cut(s) 853, 1138, 1156, 1326, 1672
CspAI ACCGGT 1 cut(s) 446
CviAII CATG 2 cut(s) 197, 392
CviQI GTAC 5 cut(s) 853, 1138, 1156, 1326, 1672
DpnI GATC 8 cut(s) 421, 481, 671, 1148, 1245, 1257, 1382, 1576
DpnII GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
DraI TTTAAA 1 cut(s) 879
Eam1104I CTCTTC 5 cut(s) 154, 493, 524, 533, 749
EarI CTCTTC 5 cut(s) 154, 493, 524, 533, 749
Ecl136II GAGCTC 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 1409
Eco24I GRGCYC 1 cut(s) 545
Eco31I GGTCTC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 1592
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 833
EcoICRI GAGCTC 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 1621
EcoT14I CCWWGG 1 cut(s) 1409
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 1409
FaeI CATG 2 cut(s) 200, 395
FalI AAGNNNNNCTT 6 cut(s) 75, 107, 100, 132, 1022, 1054
FaqI GGGAC 1 cut(s) 1570
FatI CATG 2 cut(s) 196, 391
FbaI TGATCA 1 cut(s) 1380
FblI GTMKAC 2 cut(s) 471, 1188
Fnu4HI GCNGC 3 cut(s) 156, 792, 1446
FokI GGATG 2 cut(s) 296, 800
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 3 cut(s) 156, 792, 1446
FspBI CTAG 2 cut(s) 219, 684
GluI GCNGC 3 cut(s) 156, 792, 1446
HapII CCGG 3 cut(s) 447, 1247, 1595
HgaI GACGC 1 cut(s) 419
Hin1II CATG 2 cut(s) 200, 395
HincII GTYRAC 2 cut(s) 472, 798
HindII GTYRAC 2 cut(s) 472, 798
HindIII AAGCTT 2 cut(s) 845, 885
HpaI GTTAAC 1 cut(s) 798
HpaII CCGG 3 cut(s) 447, 1247, 1595
HphI GGTGA 2 cut(s) 153, 672
Hpy166II GTNNAC 5 cut(s) 397, 472, 798, 1189, 1592
Hpy188III TCNNGA 6 cut(s) 197, 351, 377, 1039, 1216, 1247
Hpy8I GTNNAC 5 cut(s) 397, 472, 798, 1189, 1592
HpyAV CCTTC 5 cut(s) 150, 718, 983, 1115, 1489
HpyCH4III ACNGT 8 cut(s) 128, 361, 429, 461, 608, 869, 1226, 1340
HpyCH4V TGCA 7 cut(s) 97, 245, 495, 1000, 1448, 1562, 1668
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 164, 462, 540, 1237, 1471
Hsp92II CATG 2 cut(s) 200, 395
Kpn2I TCCGGA 1 cut(s) 1246
Ksp22I TGATCA 1 cut(s) 1380
KspAI GTTAAC 1 cut(s) 798
Kzo9I GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
LguI GCTCTTC 2 cut(s) 524, 533
Lsp1109I GCAGC 3 cut(s) 142, 778, 1432
LweI GCATC 3 cut(s) 357, 778, 951
MaeI CTAG 2 cut(s) 219, 684
MaeIII GTNAC 5 cut(s) 429, 863, 869, 912, 1088
MalI GATC 8 cut(s) 421, 481, 671, 1148, 1245, 1257, 1382, 1576
MboI GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
MflI RGATCY 1 cut(s) 419
MhlI GDGCHC 3 cut(s) 79, 545, 561
MluCI AATT 7 cut(s) 121, 664, 676, 761, 874, 1083, 1396
MlyI GAGTC 3 cut(s) 97, 1067, 1072
MmeI TCCRAC 1 cut(s) 387
MroI TCCGGA 1 cut(s) 1246
MroXI GAANNNNTTC 1 cut(s) 139
MseI TTAA 6 cut(s) 644, 675, 797, 836, 878, 1605
MslI CAYNNNNRTG 1 cut(s) 390
MspI CCGG 3 cut(s) 447, 1247, 1595
MspR9I CCNGG 1 cut(s) 1623
Mva1269I GAATGC 1 cut(s) 1433
MvaI CCWGG 1 cut(s) 1623
MwoI GCNNNNNNNGC 6 cut(s) 83, 164, 462, 540, 1237, 1471
NdeII GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
NlaIII CATG 2 cut(s) 200, 395
NmuCI GTSAC 2 cut(s) 869, 912
PagI TCATGA 1 cut(s) 196
PaqCI CACCTGC 1 cut(s) 172
PciSI GCTCTTC 2 cut(s) 524, 533
PctI GAATGC 1 cut(s) 1433
PdmI GAANNNNTTC 1 cut(s) 139
PfeI GAWTC 7 cut(s) 259, 329, 380, 569, 728, 1356, 1535
PinAI ACCGGT 1 cut(s) 446
PkrI GCNGC 3 cut(s) 157, 793, 1447
PleI GAGTC 3 cut(s) 96, 1067, 1072
PpsI GAGTC 3 cut(s) 96, 1067, 1072
PshAI GACNNNNGTC 1 cut(s) 1411
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 1621
PspGI CCWGG 1 cut(s) 1621
PspPI GGNCC 1 cut(s) 1592
PsuI RGATCY 1 cut(s) 419
RsaI GTAC 5 cut(s) 854, 1139, 1157, 1327, 1673
RsaNI GTAC 5 cut(s) 853, 1138, 1156, 1326, 1672
RseI CAYNNNNRTG 1 cut(s) 390
SacI GAGCTC 1 cut(s) 545
SalI GTCGAC 1 cut(s) 470
SapI GCTCTTC 2 cut(s) 524, 533
SaqAI TTAA 6 cut(s) 644, 675, 797, 836, 878, 1605
SatI GCNGC 3 cut(s) 156, 792, 1446
Sau3AI GATC 8 cut(s) 419, 479, 669, 1146, 1243, 1255, 1380, 1574
Sau96I GGNCC 1 cut(s) 1592
ScaI AGTACT 2 cut(s) 854, 1139
SchI GAGTC 3 cut(s) 97, 1067, 1072
ScrFI CCNGG 1 cut(s) 1623
SduI GDGCHC 3 cut(s) 79, 545, 561
SfaNI GCATC 3 cut(s) 357, 778, 951
SfcI CTRYAG 1 cut(s) 1336
SinI GGWCC 1 cut(s) 1592
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 2 cut(s) 1288, 1297
SmoI CTYRAG 2 cut(s) 1288, 1297
Sse9I AATT 7 cut(s) 121, 664, 676, 761, 874, 1083, 1396
SspMI CTAG 2 cut(s) 219, 684
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 1621
StyI CCWWGG 1 cut(s) 1409
TaaI ACNGT 8 cut(s) 128, 361, 429, 461, 608, 869, 1226, 1340
TaqI TCGA 6 cut(s) 471, 522, 668, 1025, 1076, 1258
TaqII GACCGA 2 cut(s) 457, 472
TasI AATT 7 cut(s) 121, 664, 676, 761, 874, 1083, 1396
TatI WGTACW 2 cut(s) 852, 1137
TfiI GAWTC 7 cut(s) 259, 329, 380, 569, 728, 1356, 1535
Tru1I TTAA 6 cut(s) 644, 675, 797, 836, 878, 1605
Tru9I TTAA 6 cut(s) 644, 675, 797, 836, 878, 1605
TscAI CASTG 2 cut(s) 972, 1345
TseFI GTSAC 2 cut(s) 869, 912
TseI GCWGC 3 cut(s) 155, 791, 1445
Tsp45I GTSAC 2 cut(s) 869, 912
TspDTI ATGAA 9 cut(s) 38, 116, 239, 240, 300, 813, 830, 1080, 1457
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 2 cut(s) 972, 1345
VpaK11BI GGWCC 1 cut(s) 1592
XmiI GTMKAC 2 cut(s) 471, 1188
XmnI GAANNNNTTC 1 cut(s) 139
XspI CTAG 2 cut(s) 219, 684
ZrmI AGTACT 2 cut(s) 854, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.