Rw5G047870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014307
Physical Location & Seq
Reverse (-)
82930652 .. 82933054
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047870.1

Sequence Viewer

Length: 1752 bp
ATGGCTACTGACACCAGAATTTTCCCAGACCAAAACAACAACAAGACCAAAAACAATGAGATTAACAGGGTGGAAATCCAAGCAGCCATTGCCAAAGCTGTGGAGCTTAGAGCTCTTCATGCTGCTTTGGTTCAAGGAAACAGCCCTGCAAATCTCAGGTTCCCTTCTTCTGCTTCCCCAGCTAATTCACGCCATGCCTCTAACTTCTCTGCTCAGGACTACCCTGTTTTCACGCCTAGTTATGAAGACGAACCATCATCCGGGTATCATCAAATTCCGATGAAGAACAGAACGTTATCCGAAAGTTGGGACGAGTACGGGCTAGAAGGAAATGGGGATGAATCAGTTGTGTCGAATTACAAGGAAAACTCATCTTCAAGAAAAGGGTTTCCTTCTGATTTATCCAACTTTGAATCCCACATTTGCCCAGCTGAGGATAACAAGTCTGTCACTGGCTCATGTACAAACAACATCACCGTGCTTCAAACATCACCAGGTACTGAGTACTTCAGGTCAAGTAGGAGAAACAGTTTGGGGGATTTCAACTCAATCTCATCTTGTAATAGATGCAAGCCTGCAACTATAACCAGTGAACCCGAAAACACATCCAGGAACAGCAGGAATTCTAACATTGTTGTGCCATTAACAGATTCTCATGCATCGACTACTACACAGTCACAACCAAAAAGTAGGGGAGTGATTTCTTGGTTGTTTCCGCGGTTAAAGAAGAAGCACAAGAATATAAGCTCTCCAACCCGGACAGAATCTGAGGAAGTTTCTCAAGCCTACAAGGATTTTGGAATCATGTCAGTTGAAGTATTGAAGAAAGAGCTCATAAAAGCAAATGAGAATAGAGACTCTGCGTTAAAGGAGGTTTCTGAGATGAGATCTTCACTTGGGGAGCTGAAGCAGAAGCTGGAGTACTTGGAAACTTACTGTGAAGAGCTGAAGAAGGCTTTGAGACAAGCAACACATGCTAATAAGAATTTCCAAATGCCAATTGGGAATTTCCCAAACAGACCAGGAAAATCAAGTGATGGGAATGCGGAAAACTTAATGCCGGTCAGCGAGGAAGTAATGGTGGAAGGTTTCCTGCAGATAGTATCCGAAGCACGACTTTCAGTAAGACAATTCTGCAAAATCCTTGTAGCACAAATTGAACTAACAGATAACACCTTAGTGGACAATTTGAACCTGCTTCTCCAACCATTCAAATTGTCATTGAATTCCAAGTATTCGAAAGCTGTGTCATACCATTTGGAAGCCATCATAAACCAGTCACTCTACCAAGACTTTGAGAACTGTGTGTTCCAGAAGCATGGCTCACCAAAGCTTCTAGATCCTCAACAAGCTCGCCAAGCGCACTGGAATGAAGTTTTGAGGAAGGGAACGAAGTATTACTGTGAGGAATTCAGCAAGTTTTGTGATCAGAAGATGAGTTGCATCATCACAACACTAAATTGGACTAGGCCATGGCCTGAGCAGGTTCTTCAAGCCTTCTTTGTAGCTACGAAATGCATTTGGTTACTTCATTTGCTTGCATTTTCATTCAATCCGCCGCTGGGGATATTGAGGGTGGAAGAGAATAGAAGCTTTGATCCACATTACATGGAAGACATGTTTATGGATAGGCAGAGATCACATGGTTCAAGCAGGGTTAAGATTATGGTAATGCCAGGGTTTTATGTTCAGGATAGGGTTTTAAGGTGTAAGGTTCTTTGCAGGTATAAGTCTGCAGGTCAAGCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

583

Amino Acids

66.06

Weight (kDa)

8.53

Isoelectric Point (pI)

58.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIL1_IRKI_C PF24994 523 - 573 9.9e-06 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12900
fragaria_vesca FvH4_3g43680 FvH4_3g43680
malus_domestica MD11G1021700.v1.1
prunus_persica Prupe.6G016100_v2.0.a1
pyrus_communis pycom03g01740 pycom11g01760
rosa_chinensis RchiOBHm_Chr5g0078341
rosa_laevigata RLG00000036768
rosa_roxburghii Rroxscaffold_1G00003900
rosa_rugosa Rorug05G0460200
rosa_samantha Rh5AG514100 Rh5BG537000 Rh5CG560500 Rh5DG548600
rosa_wichuraiana Rw5G047730 Rw5G047870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 1203, 1474, 1713, 1727
AccII CGCG 1 cut(s) 718
AciI CCGC 5 cut(s) 716, 718, 1046, 1556, 1559
AclI AACGTT 1 cut(s) 293
AclWI GGATC 2 cut(s) 1334, 1592
AcsI RAATTY 7 cut(s) 18, 273, 622, 985, 1006, 1225, 1409
AcuI CTGAAG 3 cut(s) 493, 926, 968
AfaI GTAC 5 cut(s) 317, 463, 499, 506, 923
AfiI CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1562
AflIII ACRYGT 1 cut(s) 1617
AjnI CCWGG 4 cut(s) 493, 608, 1021, 1675
AleI CACNNNNGTG 1 cut(s) 1178
Alw21I GWGCWC 2 cut(s) 115, 834
Alw26I GTCTC 2 cut(s) 849, 955
AlwI GGATC 2 cut(s) 1334, 1592
AlwNI CAGNNNCTG 3 cut(s) 500, 767, 916
AoxI GGCC 2 cut(s) 1469, 1475
ApeKI GCWGC 2 cut(s) 83, 122
ApoI RAATTY 7 cut(s) 18, 273, 622, 985, 1006, 1225, 1409
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
AspLEI GCGC 1 cut(s) 1363
AsuC2I CCSGG 2 cut(s) 262, 757
AsuHPI GGTGA 3 cut(s) 466, 483, 1317
AsuII TTCGAA 1 cut(s) 1238
BanII GRGCYC 2 cut(s) 115, 834
BarI GAAGNNNNNNTAC 2 cut(s) 905, 937
BbsI GAAGAC 2 cut(s) 252, 1620
Bbv12I GWGCWC 2 cut(s) 115, 834
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 95, 109
BccI CCATC 3 cut(s) 262, 1031, 1274
BciT130I CCWGG 4 cut(s) 495, 610, 1023, 1677
BciVI GTATCC 1 cut(s) 1114
BclI TGATCA 1 cut(s) 1426
BcnI CCSGG 2 cut(s) 262, 757
BcoDI GTCTC 2 cut(s) 849, 955
BfaI CTAG 4 cut(s) 237, 323, 1337, 1467
BfmI CTRYAG 2 cut(s) 1094, 1734
BfuAI ACCTGC 4 cut(s) 1203, 1474, 1713, 1727
BfuI GTATCC 1 cut(s) 1114
BglII AGATCT 1 cut(s) 887
BisI GCNGC 3 cut(s) 84, 123, 1559
BlsI GCNGC 3 cut(s) 85, 124, 1560
BmcAI AGTACT 2 cut(s) 506, 923
Bme1390I CCNGG 6 cut(s) 262, 495, 610, 757, 1023, 1677
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 6 cut(s) 262, 495, 610, 757, 1023, 1677
BmsI GCATC 3 cut(s) 557, 668, 1452
BpiI GAAGAC 2 cut(s) 252, 1620
BpmI CTGGAG 1 cut(s) 938
Bpu10I CCTNAGC 3 cut(s) 213, 432, 1479
Bpu14I TTCGAA 1 cut(s) 1238
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 2 cut(s) 262, 757
BsaJI CCNNGG 3 cut(s) 716, 1472, 1676
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1562
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 4 cut(s) 457, 588, 1276, 1370
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 4 cut(s) 495, 610, 1023, 1677
BseDI CCNNGG 3 cut(s) 716, 1472, 1676
BseGI GGATG 3 cut(s) 257, 343, 605
BseLI CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1562
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 7 cut(s) 169, 227, 423, 492, 759, 870, 1470
BseNI ACTGG 4 cut(s) 457, 588, 1276, 1370
BseXI GCAGC 2 cut(s) 95, 109
BseYI CCCAGC 3 cut(s) 178, 427, 1561
Bsh1236I CGCG 1 cut(s) 718
BshFI GGCC 2 cut(s) 1471, 1477
BsiHKAI GWGCWC 2 cut(s) 115, 834
BsiSI CCGG 3 cut(s) 261, 757, 1061
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1562
BsmAI GTCTC 2 cut(s) 849, 955
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 1048
BsnI GGCC 2 cut(s) 1471, 1477
Bsp119I TTCGAA 1 cut(s) 1238
Bsp1286I GDGCHC 2 cut(s) 115, 834
Bsp1407I TGTACA 1 cut(s) 461
Bsp143I GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
Bsp19I CCATGG 1 cut(s) 1472
BspACI CCGC 5 cut(s) 716, 718, 1046, 1556, 1559
BspANI GGCC 2 cut(s) 1471, 1477
BspCNI CTCAG 7 cut(s) 168, 226, 424, 493, 760, 871, 1471
BspFNI CGCG 1 cut(s) 718
BspLI GGNNCC 1 cut(s) 161
BspMAI CTGCAG 2 cut(s) 1098, 1738
BspMI ACCTGC 4 cut(s) 1203, 1474, 1713, 1727
BspPI GGATC 2 cut(s) 1334, 1592
BspQI GCTCTTC 2 cut(s) 120, 936
BspT104I TTCGAA 1 cut(s) 1238
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 1060
BsrGI TGTACA 1 cut(s) 461
BsrI ACTGG 4 cut(s) 457, 588, 1276, 1370
BssAI RCCGGY 1 cut(s) 1060
BssECI CCNNGG 3 cut(s) 716, 1472, 1676
BssMI GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
BssT1I CCWWGG 1 cut(s) 1472
Bst2UI CCWGG 4 cut(s) 495, 610, 1023, 1677
Bst4CI ACNGT 6 cut(s) 478, 530, 675, 938, 1304, 1403
Bst6I CTCTTC 3 cut(s) 120, 936, 1575
BstAPI GCANNNNNTGC 2 cut(s) 89, 974
BstAUI TGTACA 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 1238
BstC8I GCNNGC 4 cut(s) 572, 576, 1354, 1539
BstDEI CTNAG 9 cut(s) 107, 155, 213, 432, 501, 768, 879, 1177, 1479
BstDSI CCRYGG 2 cut(s) 716, 1472
BstF5I GGATG 3 cut(s) 257, 343, 605
BstFNI CGCG 1 cut(s) 718
BstHHI GCGC 1 cut(s) 1363
BstKTI GATC 5 cut(s) 890, 1342, 1429, 1600, 1640
BstMAI GTCTC 2 cut(s) 849, 955
BstMBI GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
BstMWI GCNNNNNNNGC 6 cut(s) 89, 119, 179, 974, 1358, 1742
BstNI CCWGG 4 cut(s) 495, 610, 1023, 1677
BstNSI RCATGY 2 cut(s) 977, 1621
BstSCI CCNGG 6 cut(s) 260, 493, 608, 755, 1021, 1675
BstSFI CTRYAG 2 cut(s) 1094, 1734
BstUI CGCG 1 cut(s) 718
BstV1I GCAGC 2 cut(s) 95, 109
BstV2I GAAGAC 2 cut(s) 252, 1620
BstX2I RGATCY 2 cut(s) 887, 1339
BstXI CCANNNNNNTGG 2 cut(s) 100, 1319
BstYI RGATCY 2 cut(s) 887, 1339
BsuI GTATCC 1 cut(s) 1114
BsuRI GGCC 2 cut(s) 1471, 1477
BtgI CCRYGG 2 cut(s) 716, 1472
BtsCI GGATG 3 cut(s) 257, 343, 605
BtsIMutI CAGTG 3 cut(s) 450, 595, 1363
BveI ACCTGC 4 cut(s) 1203, 1474, 1713, 1727
Cac8I GCNNGC 4 cut(s) 572, 576, 1354, 1539
CaiI CAGNNNCTG 3 cut(s) 500, 767, 916
CfoI GCGC 1 cut(s) 1363
Cfr10I RCCGGY 1 cut(s) 1060
Cfr42I CCGCGG 1 cut(s) 719
CsiI ACCWGGT 1 cut(s) 493
Csp6I GTAC 5 cut(s) 316, 462, 498, 505, 922
CviQI GTAC 5 cut(s) 316, 462, 498, 505, 922
DdeI CTNAG 9 cut(s) 107, 155, 213, 432, 501, 768, 879, 1177, 1479
DpnI GATC 5 cut(s) 889, 1341, 1428, 1599, 1639
DpnII GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
Eam1104I CTCTTC 3 cut(s) 120, 936, 1575
EarI CTCTTC 3 cut(s) 120, 936, 1575
EciI GGCGGA 1 cut(s) 1545
Ecl136II GAGCTC 2 cut(s) 113, 832
Eco130I CCWWGG 1 cut(s) 1472
Eco24I GRGCYC 2 cut(s) 115, 834
Eco53kI GAGCTC 2 cut(s) 113, 832
Eco57I CTGAAG 3 cut(s) 493, 926, 968
EcoICRI GAGCTC 2 cut(s) 113, 832
EcoRI GAATTC 3 cut(s) 622, 1225, 1409
EcoRII CCWGG 4 cut(s) 493, 608, 1021, 1675
EcoT14I CCWWGG 1 cut(s) 1472
EcoT22I ATGCAT 2 cut(s) 661, 1520
EcoT38I GRGCYC 2 cut(s) 115, 834
ErhI CCWWGG 1 cut(s) 1472
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 1 cut(s) 323
FbaI TGATCA 1 cut(s) 1426
Fnu4HI GCNGC 3 cut(s) 84, 123, 1559
FokI GGATG 3 cut(s) 244, 350, 592
FriOI GRGCYC 2 cut(s) 115, 834
Fsp4HI GCNGC 3 cut(s) 84, 123, 1559
FspBI CTAG 4 cut(s) 237, 323, 1337, 1467
GlaI GCGC 1 cut(s) 1362
GluI GCNGC 3 cut(s) 84, 123, 1559
GsaI CCCAGC 3 cut(s) 182, 431, 1565
GsuI CTGGAG 1 cut(s) 938
HaeIII GGCC 2 cut(s) 1471, 1477
HapII CCGG 3 cut(s) 261, 757, 1061
HhaI GCGC 1 cut(s) 1363
Hin6I GCGC 1 cut(s) 1361
HinP1I GCGC 1 cut(s) 1361
HindIII AAGCTT 2 cut(s) 1331, 1591
HinfI GANTC 6 cut(s) 341, 413, 650, 764, 801, 857
HpaII CCGG 3 cut(s) 261, 757, 1061
HphI GGTGA 3 cut(s) 466, 483, 1317
Hpy166II GTNNAC 2 cut(s) 593, 1183
Hpy188I TCNGA 7 cut(s) 279, 301, 397, 769, 880, 1108, 1431
Hpy188III TCNNGA 5 cut(s) 215, 378, 1312, 1337, 1691
Hpy8I GTNNAC 2 cut(s) 593, 1183
HpyAV CCTTC 7 cut(s) 174, 320, 402, 946, 1079, 1378, 1507
HpyCH4III ACNGT 6 cut(s) 478, 530, 675, 938, 1304, 1403
HpyCH4IV ACGT 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 119, 179, 974, 1358, 1742
HpyF3I CTNAG 9 cut(s) 107, 155, 213, 432, 501, 768, 879, 1177, 1479
HpySE526I ACGT 1 cut(s) 293
HspAI GCGC 1 cut(s) 1361
Ksp22I TGATCA 1 cut(s) 1426
KspI CCGCGG 1 cut(s) 719
Kzo9I GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
LguI GCTCTTC 2 cut(s) 120, 936
LmnI GCTCC 2 cut(s) 103, 901
Lsp1109I GCAGC 2 cut(s) 95, 109
LweI GCATC 3 cut(s) 557, 668, 1452
MabI ACCWGGT 1 cut(s) 493
MaeI CTAG 4 cut(s) 237, 323, 1337, 1467
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 4 cut(s) 448, 675, 1278, 1524
MalI GATC 5 cut(s) 889, 1341, 1428, 1599, 1639
MboI GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 2 cut(s) 887, 1339
MhlI GDGCHC 2 cut(s) 115, 834
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 3 cut(s) 429, 776, 1228
MnlI CCTC 9 cut(s) 208, 427, 763, 865, 1063, 1353, 1374, 1399, 1566
Mph1103I ATGCAT 2 cut(s) 661, 1520
MseI TTAA 7 cut(s) 63, 644, 722, 866, 1055, 1659, 1703
MslI CAYNNNNRTG 5 cut(s) 476, 635, 1178, 1368, 1622
MspA1I CMGCKG 3 cut(s) 431, 718, 1561
MspI CCGG 3 cut(s) 261, 757, 1061
MspR9I CCNGG 6 cut(s) 262, 495, 610, 757, 1023, 1677
MunI CAATTG 1 cut(s) 999
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 4 cut(s) 495, 610, 1023, 1677
MvnI CGCG 1 cut(s) 718
MwoI GCNNNNNNNGC 6 cut(s) 89, 119, 179, 974, 1358, 1742
NciI CCSGG 2 cut(s) 262, 757
NcoI CCATGG 1 cut(s) 1472
NdeII GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
NlaIV GGNNCC 1 cut(s) 161
NmuCI GTSAC 3 cut(s) 448, 675, 1278
NsiI ATGCAT 2 cut(s) 661, 1520
NspI RCATGY 2 cut(s) 977, 1621
NspV TTCGAA 1 cut(s) 1238
OliI CACNNNNGTG 1 cut(s) 1178
PciI ACATGT 1 cut(s) 1617
PciSI GCTCTTC 2 cut(s) 120, 936
PctI GAATGC 1 cut(s) 1048
PfeI GAWTC 5 cut(s) 341, 413, 650, 764, 801
PfoI TCCNGGA 1 cut(s) 608
PkrI GCNGC 3 cut(s) 85, 124, 1560
PleI GAGTC 1 cut(s) 851
PpsI GAGTC 1 cut(s) 851
PscI ACATGT 1 cut(s) 1617
Psp124BI GAGCTC 2 cut(s) 115, 834
Psp1406I AACGTT 1 cut(s) 293
Psp6I CCWGG 4 cut(s) 493, 608, 1021, 1675
PspFI CCCAGC 3 cut(s) 178, 427, 1561
PspGI CCWGG 4 cut(s) 493, 608, 1021, 1675
PspN4I GGNNCC 1 cut(s) 161
PstI CTGCAG 2 cut(s) 1098, 1738
PstNI CAGNNNCTG 3 cut(s) 500, 767, 916
PsuI RGATCY 2 cut(s) 887, 1339
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 5 cut(s) 317, 463, 499, 506, 923
RsaNI GTAC 5 cut(s) 316, 462, 498, 505, 922
RseI CAYNNNNRTG 5 cut(s) 476, 635, 1178, 1368, 1622
SacI GAGCTC 2 cut(s) 115, 834
SacII CCGCGG 1 cut(s) 719
SapI GCTCTTC 2 cut(s) 120, 936
SaqAI TTAA 7 cut(s) 63, 644, 722, 866, 1055, 1659, 1703
SatI GCNGC 3 cut(s) 84, 123, 1559
Sau3AI GATC 5 cut(s) 887, 1339, 1426, 1597, 1637
ScaI AGTACT 2 cut(s) 506, 923
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 6 cut(s) 262, 495, 610, 757, 1023, 1677
SduI GDGCHC 2 cut(s) 115, 834
SexAI ACCWGGT 1 cut(s) 493
SfaNI GCATC 3 cut(s) 557, 668, 1452
SfcI CTRYAG 2 cut(s) 1094, 1734
Sfr303I CCGCGG 1 cut(s) 719
SfuI TTCGAA 1 cut(s) 1238
SgrBI CCGCGG 1 cut(s) 719
SmiMI CAYNNNNRTG 5 cut(s) 476, 635, 1178, 1368, 1622
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
SsiI CCGC 5 cut(s) 716, 718, 1046, 1556, 1559
SspMI CTAG 4 cut(s) 237, 323, 1337, 1467
SstI GAGCTC 2 cut(s) 115, 834
StyD4I CCNGG 6 cut(s) 260, 493, 608, 755, 1021, 1675
StyI CCWWGG 1 cut(s) 1472
TaaI ACNGT 6 cut(s) 478, 530, 675, 938, 1304, 1403
TaiI ACGT 1 cut(s) 296
TaqI TCGA 3 cut(s) 353, 662, 1238
TatI WGTACW 3 cut(s) 461, 504, 921
TauI GCSGC 1 cut(s) 1561
TfiI GAWTC 5 cut(s) 341, 413, 650, 764, 801
Tru1I TTAA 7 cut(s) 63, 644, 722, 866, 1055, 1659, 1703
Tru9I TTAA 7 cut(s) 63, 644, 722, 866, 1055, 1659, 1703
TscAI CASTG 3 cut(s) 457, 595, 1370
TseFI GTSAC 3 cut(s) 448, 675, 1278
TseI GCWGC 2 cut(s) 83, 122
Tsp45I GTSAC 3 cut(s) 448, 675, 1278
TspDTI ATGAA 7 cut(s) 107, 258, 296, 354, 1386, 1520, 1536
TspRI CASTG 3 cut(s) 457, 595, 1370
XapI RAATTY 7 cut(s) 18, 273, 622, 985, 1006, 1225, 1409
XbaI TCTAGA 1 cut(s) 1336
XceI RCATGY 2 cut(s) 977, 1621
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XspI CTAG 4 cut(s) 237, 323, 1337, 1467
ZrmI AGTACT 2 cut(s) 506, 923
Zsp2I ATGCAT 2 cut(s) 661, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.