Rw5G047730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014307
Physical Location & Seq
Reverse (-)
82715709 .. 82718414
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047730.1

Sequence Viewer

Length: 1791 bp
ATGGCTACTGACACCAGAATTTTCCCAGACCAAAACAACAACAAGACCAAAAACAATGAGATTAACAGGGTGGAAATCCAAGCAGCCATTGCCAAAGCTGTGGAGCTTAGAGCTCTTCATGCTGCTTTGGTTCAAGGAAACAGCCCTGCAAATCTCAGGTTCCCTTCTTCTGCTTCCCCAGCTAATTCACGCCATGCCTCTAACTTCTCTGCTCAGGACTACCCTGTTTTCACGCCTAGTTATGAAGACGAACCATCATCCGGGTATCATCAAATTCCGATGAAGAACAGAACGTTATCCGAAAGTTGGGACGAGTACGGGCTAGAAGGAAATGGGGATGAATCAGTTGTGTCGAATTACAAGGAAAACTCATCTTCAAGAAAAGGGTTTCCTTCTGATTTATCCAACTTTGAATCCCACATTTGCCCAGCTGAGGATAACAAGTCTGTCACTGGCTCATGTACAAACAACATCACCGTGCTTCAAACATCACCAGGTACTGAGTACTTCAGGTCAAGTAGGAGAAACAGTTTGGGGGATTTCAACTCAATCTCATCTTGTAATAGATGCAAGCCTGCAACTATAACCAGTGAACCCGAAAACACATCCAGGAACAGCAGGAATTCTAACATTGTTGTGCCATTAACAGATTCTCATGCATCGACTACTACACAGTCACAACCAAAAAGTAGGGGAGTGATTTCTTGGTTGTTTCCGCGGTTAAAGAAGAAGCACAAGAATATAAGCTCTCCAACCCGGACAGAATCTGAGGAAGTTTCTCAAGCCTACAAGGATTTTGGAATCATGTCAGTTGAAGTATTGAAGAAAGAGCTCATAAAAGCAAATGAGAATAGAGACTCTGCGTTAAAGGAGGTTTCTGAGATGAGATCTTCACTTGGGGAGCTGAAGCAGAAGCTGGAGTACTTGGAAACTTACTGTGAAGAGCTGAAGAAGGCTTTGAGACAAGCAACACATGCTAATAAGAATTTCCAAATGCCAGAAAATATTGGGAATTTCCCAAACAGACCAGGAAAATCAAGTGATGGGAATGCGGAAAACTTAATGCCGGTCAGCGAGGAAGTAATGGTGGAAGGTTTCCTGCAGATAGTATCCGAAGCACGACTTTCAGTAAGACAATTCTGCAAAATCCTTGTAGCACAAATTGAACTAACAGATAACACCTTAGTGGAAAATTTGAACCTGCTTCTCCAACCATTCAAATTGTCATTGAATTCCAAGTATTCGAAAGCTGTGTCATACCATTTGGAAGCCATCATAAACCAGTCACTCTACCAAGACTTTGAGAACTGTGTGTTCCAGAAGCATGGCTCACCAAAGCTTCTAGATCCTCAACAAGCTCGCCAAGCGCAGTTTTCTTCATTTGTGGCACTAAGAAACTTAAGCTGGAATGAAGTTTTGAGGAAGGGAACGAAGTATTACTGTGAGGAATTCAGCAAGTTTTGTGATCAGAAGATGAGTTGCATCATCACAACACTAAATTGGACTAGGCCATGGCCTGAGCAGGTTCTTCAAGCCTTCTTTGTAGCTACGAAATGCATTTGGTTACTTCATTTGCTTGCATTTTCATTCAATCCGCCGCTGGGGATATTGAGGGTGGAAGAGAATAGAAGCTTTGATCCACATTACATGGAAGACATGTTTATGGATAGGCAGAGATCACATGGTTCAAGCAGGGTTAAGATTATGGTAATGCCAGGGTTTTATGTTCAGGATAGGGTTTTAAGGTGTAAGGTTCTTTGCAGGTATAAGTCTGCAGGTCAAGCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

596

Amino Acids

67.53

Weight (kDa)

8.53

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIL1_IRKI_C PF24994 536 - 586 1e-05 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12900
fragaria_vesca FvH4_3g43680 FvH4_3g43680
malus_domestica MD11G1021700.v1.1
prunus_persica Prupe.6G016100_v2.0.a1
pyrus_communis pycom03g01740 pycom11g01760
rosa_chinensis RchiOBHm_Chr5g0078341
rosa_laevigata RLG00000036768
rosa_roxburghii Rroxscaffold_1G00003900
rosa_rugosa Rorug05G0460200
rosa_samantha Rh5AG514100 Rh5BG537000 Rh5CG560500 Rh5DG548600
rosa_wichuraiana Rw5G047730 Rw5G047870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 1209, 1513, 1752, 1766
AccII CGCG 1 cut(s) 718
AciI CCGC 5 cut(s) 716, 718, 1052, 1595, 1598
AclI AACGTT 1 cut(s) 293
AclWI GGATC 2 cut(s) 1340, 1631
AcsI RAATTY 8 cut(s) 18, 273, 622, 985, 1012, 1192, 1231, 1448
AcuI CTGAAG 3 cut(s) 493, 926, 968
AfaI GTAC 5 cut(s) 317, 463, 499, 506, 923
AfiI CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1601
AflII CTTAAG 1 cut(s) 1399
AflIII ACRYGT 1 cut(s) 1656
AjnI CCWGG 4 cut(s) 493, 608, 1027, 1714
AleI CACNNNNGTG 1 cut(s) 1184
Alw21I GWGCWC 2 cut(s) 115, 834
Alw26I GTCTC 2 cut(s) 849, 955
AlwI GGATC 2 cut(s) 1340, 1631
AlwNI CAGNNNCTG 3 cut(s) 500, 767, 916
AoxI GGCC 2 cut(s) 1508, 1514
ApeKI GCWGC 2 cut(s) 83, 122
ApoI RAATTY 8 cut(s) 18, 273, 622, 985, 1012, 1192, 1231, 1448
ArsI GACNNNNNNTTYG 2 cut(s) 1238, 1270
AspLEI GCGC 1 cut(s) 1369
AsuC2I CCSGG 2 cut(s) 262, 757
AsuHPI GGTGA 3 cut(s) 466, 483, 1323
AsuII TTCGAA 1 cut(s) 1244
BanII GRGCYC 2 cut(s) 115, 834
BarI GAAGNNNNNNTAC 2 cut(s) 905, 937
BbsI GAAGAC 2 cut(s) 252, 1659
Bbv12I GWGCWC 2 cut(s) 115, 834
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 95, 109
BccI CCATC 3 cut(s) 262, 1037, 1280
BciT130I CCWGG 4 cut(s) 495, 610, 1029, 1716
BciVI GTATCC 1 cut(s) 1120
BclI TGATCA 1 cut(s) 1465
BcnI CCSGG 2 cut(s) 262, 757
BcoDI GTCTC 2 cut(s) 849, 955
BfaI CTAG 4 cut(s) 237, 323, 1343, 1506
BfmI CTRYAG 2 cut(s) 1100, 1773
BfrI CTTAAG 1 cut(s) 1399
BfuAI ACCTGC 4 cut(s) 1209, 1513, 1752, 1766
BfuI GTATCC 1 cut(s) 1120
BglII AGATCT 1 cut(s) 887
BisI GCNGC 3 cut(s) 84, 123, 1598
BlsI GCNGC 3 cut(s) 85, 124, 1599
BmcAI AGTACT 2 cut(s) 506, 923
Bme1390I CCNGG 6 cut(s) 262, 495, 610, 757, 1029, 1716
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 6 cut(s) 262, 495, 610, 757, 1029, 1716
BmsI GCATC 3 cut(s) 557, 668, 1491
BpiI GAAGAC 2 cut(s) 252, 1659
BpmI CTGGAG 1 cut(s) 938
Bpu10I CCTNAGC 3 cut(s) 213, 432, 1518
Bpu14I TTCGAA 1 cut(s) 1244
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 2 cut(s) 262, 757
BsaJI CCNNGG 3 cut(s) 716, 1511, 1715
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1601
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 3 cut(s) 457, 588, 1282
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 4 cut(s) 495, 610, 1029, 1716
BseDI CCNNGG 3 cut(s) 716, 1511, 1715
BseGI GGATG 3 cut(s) 257, 343, 605
BseLI CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1601
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 7 cut(s) 169, 227, 423, 492, 759, 870, 1509
BseNI ACTGG 3 cut(s) 457, 588, 1282
BseXI GCAGC 2 cut(s) 95, 109
BseYI CCCAGC 3 cut(s) 178, 427, 1600
Bsh1236I CGCG 1 cut(s) 718
BshFI GGCC 2 cut(s) 1510, 1516
BsiHKAI GWGCWC 2 cut(s) 115, 834
BsiSI CCGG 3 cut(s) 261, 757, 1067
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 4 cut(s) 260, 306, 433, 1601
BsmAI GTCTC 2 cut(s) 849, 955
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 1054
BsnI GGCC 2 cut(s) 1510, 1516
Bsp119I TTCGAA 1 cut(s) 1244
Bsp1286I GDGCHC 2 cut(s) 115, 834
Bsp1407I TGTACA 1 cut(s) 461
Bsp143I GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
Bsp19I CCATGG 1 cut(s) 1511
BspACI CCGC 5 cut(s) 716, 718, 1052, 1595, 1598
BspANI GGCC 2 cut(s) 1510, 1516
BspCNI CTCAG 7 cut(s) 168, 226, 424, 493, 760, 871, 1510
BspFNI CGCG 1 cut(s) 718
BspLI GGNNCC 1 cut(s) 161
BspMAI CTGCAG 2 cut(s) 1104, 1777
BspMI ACCTGC 4 cut(s) 1209, 1513, 1752, 1766
BspPI GGATC 2 cut(s) 1340, 1631
BspQI GCTCTTC 2 cut(s) 120, 936
BspT104I TTCGAA 1 cut(s) 1244
BspTI CTTAAG 1 cut(s) 1399
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 1066
BsrGI TGTACA 1 cut(s) 461
BsrI ACTGG 3 cut(s) 457, 588, 1282
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 3 cut(s) 716, 1511, 1715
BssMI GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
BssT1I CCWWGG 1 cut(s) 1511
Bst2UI CCWGG 4 cut(s) 495, 610, 1029, 1716
Bst4CI ACNGT 6 cut(s) 478, 530, 675, 938, 1310, 1442
Bst6I CTCTTC 3 cut(s) 120, 936, 1614
BstAFI CTTAAG 1 cut(s) 1399
BstAPI GCANNNNNTGC 2 cut(s) 89, 974
BstAUI TGTACA 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 1244
BstC8I GCNNGC 4 cut(s) 572, 576, 1360, 1578
BstDSI CCRYGG 2 cut(s) 716, 1511
BstF5I GGATG 3 cut(s) 257, 343, 605
BstFNI CGCG 1 cut(s) 718
BstHHI GCGC 1 cut(s) 1369
BstKTI GATC 5 cut(s) 890, 1348, 1468, 1639, 1679
BstMAI GTCTC 2 cut(s) 849, 955
BstMBI GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
BstMWI GCNNNNNNNGC 6 cut(s) 89, 119, 179, 974, 1364, 1781
BstNI CCWGG 4 cut(s) 495, 610, 1029, 1716
BstNSI RCATGY 2 cut(s) 977, 1660
BstSCI CCNGG 6 cut(s) 260, 493, 608, 755, 1027, 1714
BstSFI CTRYAG 2 cut(s) 1100, 1773
BstUI CGCG 1 cut(s) 718
BstV1I GCAGC 2 cut(s) 95, 109
BstV2I GAAGAC 2 cut(s) 252, 1659
BstX2I RGATCY 2 cut(s) 887, 1345
BstXI CCANNNNNNTGG 2 cut(s) 100, 1325
BstYI RGATCY 2 cut(s) 887, 1345
BsuI GTATCC 1 cut(s) 1120
BsuRI GGCC 2 cut(s) 1510, 1516
BtgI CCRYGG 2 cut(s) 716, 1511
BtsCI GGATG 3 cut(s) 257, 343, 605
BtsIMutI CAGTG 2 cut(s) 450, 595
BveI ACCTGC 4 cut(s) 1209, 1513, 1752, 1766
Cac8I GCNNGC 4 cut(s) 572, 576, 1360, 1578
CaiI CAGNNNCTG 3 cut(s) 500, 767, 916
CfoI GCGC 1 cut(s) 1369
Cfr10I RCCGGY 1 cut(s) 1066
Cfr42I CCGCGG 1 cut(s) 719
CsiI ACCWGGT 1 cut(s) 493
Csp6I GTAC 5 cut(s) 316, 462, 498, 505, 922
CviQI GTAC 5 cut(s) 316, 462, 498, 505, 922
DpnI GATC 5 cut(s) 889, 1347, 1467, 1638, 1678
DpnII GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
Eam1104I CTCTTC 3 cut(s) 120, 936, 1614
EarI CTCTTC 3 cut(s) 120, 936, 1614
EciI GGCGGA 1 cut(s) 1584
Ecl136II GAGCTC 2 cut(s) 113, 832
Eco130I CCWWGG 1 cut(s) 1511
Eco24I GRGCYC 2 cut(s) 115, 834
Eco53kI GAGCTC 2 cut(s) 113, 832
Eco57I CTGAAG 3 cut(s) 493, 926, 968
EcoICRI GAGCTC 2 cut(s) 113, 832
EcoRI GAATTC 3 cut(s) 622, 1231, 1448
EcoRII CCWGG 4 cut(s) 493, 608, 1027, 1714
EcoT14I CCWWGG 1 cut(s) 1511
EcoT22I ATGCAT 2 cut(s) 661, 1559
EcoT38I GRGCYC 2 cut(s) 115, 834
ErhI CCWWGG 1 cut(s) 1511
FalI AAGNNNNNCTT 2 cut(s) 1107, 1139
FaqI GGGAC 1 cut(s) 323
FbaI TGATCA 1 cut(s) 1465
Fnu4HI GCNGC 3 cut(s) 84, 123, 1598
FokI GGATG 3 cut(s) 244, 350, 592
FriOI GRGCYC 2 cut(s) 115, 834
Fsp4HI GCNGC 3 cut(s) 84, 123, 1598
FspBI CTAG 4 cut(s) 237, 323, 1343, 1506
GlaI GCGC 1 cut(s) 1368
GluI GCNGC 3 cut(s) 84, 123, 1598
GsaI CCCAGC 3 cut(s) 182, 431, 1604
GsuI CTGGAG 1 cut(s) 938
HaeIII GGCC 2 cut(s) 1510, 1516
HapII CCGG 3 cut(s) 261, 757, 1067
HhaI GCGC 1 cut(s) 1369
Hin6I GCGC 1 cut(s) 1367
HinP1I GCGC 1 cut(s) 1367
HindIII AAGCTT 2 cut(s) 1337, 1630
HinfI GANTC 6 cut(s) 341, 413, 650, 764, 801, 857
HpaII CCGG 3 cut(s) 261, 757, 1067
HphI GGTGA 3 cut(s) 466, 483, 1323
Hpy166II GTNNAC 1 cut(s) 593
Hpy188I TCNGA 7 cut(s) 279, 301, 397, 769, 880, 1114, 1470
Hpy188III TCNNGA 5 cut(s) 215, 378, 1318, 1343, 1730
Hpy8I GTNNAC 1 cut(s) 593
HpyAV CCTTC 7 cut(s) 174, 320, 402, 946, 1085, 1417, 1546
HpyCH4III ACNGT 6 cut(s) 478, 530, 675, 938, 1310, 1442
HpyCH4IV ACGT 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 119, 179, 974, 1364, 1781
HpySE526I ACGT 1 cut(s) 293
HspAI GCGC 1 cut(s) 1367
Ksp22I TGATCA 1 cut(s) 1465
KspI CCGCGG 1 cut(s) 719
Kzo9I GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
LguI GCTCTTC 2 cut(s) 120, 936
LmnI GCTCC 2 cut(s) 103, 901
Lsp1109I GCAGC 2 cut(s) 95, 109
LweI GCATC 3 cut(s) 557, 668, 1491
MabI ACCWGGT 1 cut(s) 493
MaeI CTAG 4 cut(s) 237, 323, 1343, 1506
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 4 cut(s) 448, 675, 1284, 1563
MalI GATC 5 cut(s) 889, 1347, 1467, 1638, 1678
MboI GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
MflI RGATCY 2 cut(s) 887, 1345
MhlI GDGCHC 2 cut(s) 115, 834
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 3 cut(s) 429, 776, 1234
MnlI CCTC 9 cut(s) 208, 427, 763, 865, 1069, 1359, 1413, 1438, 1605
Mph1103I ATGCAT 2 cut(s) 661, 1559
MseI TTAA 8 cut(s) 63, 644, 722, 866, 1061, 1400, 1698, 1742
MslI CAYNNNNRTG 4 cut(s) 476, 635, 1184, 1661
MspA1I CMGCKG 3 cut(s) 431, 718, 1600
MspCI CTTAAG 1 cut(s) 1399
MspI CCGG 3 cut(s) 261, 757, 1067
MspR9I CCNGG 6 cut(s) 262, 495, 610, 757, 1029, 1716
Mva1269I GAATGC 1 cut(s) 1054
MvaI CCWGG 4 cut(s) 495, 610, 1029, 1716
MvnI CGCG 1 cut(s) 718
MwoI GCNNNNNNNGC 6 cut(s) 89, 119, 179, 974, 1364, 1781
NciI CCSGG 2 cut(s) 262, 757
NcoI CCATGG 1 cut(s) 1511
NdeII GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
NlaIV GGNNCC 1 cut(s) 161
NmuCI GTSAC 3 cut(s) 448, 675, 1284
NsiI ATGCAT 2 cut(s) 661, 1559
NspI RCATGY 2 cut(s) 977, 1660
NspV TTCGAA 1 cut(s) 1244
OliI CACNNNNGTG 1 cut(s) 1184
PciI ACATGT 1 cut(s) 1656
PciSI GCTCTTC 2 cut(s) 120, 936
PctI GAATGC 1 cut(s) 1054
PfeI GAWTC 5 cut(s) 341, 413, 650, 764, 801
PfoI TCCNGGA 1 cut(s) 608
PkrI GCNGC 3 cut(s) 85, 124, 1599
PleI GAGTC 1 cut(s) 851
PpsI GAGTC 1 cut(s) 851
PscI ACATGT 1 cut(s) 1656
Psp124BI GAGCTC 2 cut(s) 115, 834
Psp1406I AACGTT 1 cut(s) 293
Psp6I CCWGG 4 cut(s) 493, 608, 1027, 1714
PspFI CCCAGC 3 cut(s) 178, 427, 1600
PspGI CCWGG 4 cut(s) 493, 608, 1027, 1714
PspN4I GGNNCC 1 cut(s) 161
PstI CTGCAG 2 cut(s) 1104, 1777
PstNI CAGNNNCTG 3 cut(s) 500, 767, 916
PsuI RGATCY 2 cut(s) 887, 1345
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 5 cut(s) 317, 463, 499, 506, 923
RsaNI GTAC 5 cut(s) 316, 462, 498, 505, 922
RseI CAYNNNNRTG 4 cut(s) 476, 635, 1184, 1661
SacI GAGCTC 2 cut(s) 115, 834
SacII CCGCGG 1 cut(s) 719
SapI GCTCTTC 2 cut(s) 120, 936
SaqAI TTAA 8 cut(s) 63, 644, 722, 866, 1061, 1400, 1698, 1742
SatI GCNGC 3 cut(s) 84, 123, 1598
Sau3AI GATC 5 cut(s) 887, 1345, 1465, 1636, 1676
ScaI AGTACT 2 cut(s) 506, 923
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 6 cut(s) 262, 495, 610, 757, 1029, 1716
SduI GDGCHC 2 cut(s) 115, 834
SexAI ACCWGGT 1 cut(s) 493
SfaNI GCATC 3 cut(s) 557, 668, 1491
SfcI CTRYAG 2 cut(s) 1100, 1773
Sfr303I CCGCGG 1 cut(s) 719
SfuI TTCGAA 1 cut(s) 1244
SgrBI CCGCGG 1 cut(s) 719
SmiMI CAYNNNNRTG 4 cut(s) 476, 635, 1184, 1661
SmlI CTYRAG 2 cut(s) 780, 1399
SmoI CTYRAG 2 cut(s) 780, 1399
SsiI CCGC 5 cut(s) 716, 718, 1052, 1595, 1598
SspI AATATT 1 cut(s) 1006
SspMI CTAG 4 cut(s) 237, 323, 1343, 1506
SstI GAGCTC 2 cut(s) 115, 834
StyD4I CCNGG 6 cut(s) 260, 493, 608, 755, 1027, 1714
StyI CCWWGG 1 cut(s) 1511
TaaI ACNGT 6 cut(s) 478, 530, 675, 938, 1310, 1442
TaiI ACGT 1 cut(s) 296
TaqI TCGA 3 cut(s) 353, 662, 1244
TatI WGTACW 3 cut(s) 461, 504, 921
TauI GCSGC 1 cut(s) 1600
TfiI GAWTC 5 cut(s) 341, 413, 650, 764, 801
Tru1I TTAA 8 cut(s) 63, 644, 722, 866, 1061, 1400, 1698, 1742
Tru9I TTAA 8 cut(s) 63, 644, 722, 866, 1061, 1400, 1698, 1742
TscAI CASTG 2 cut(s) 457, 595
TseFI GTSAC 3 cut(s) 448, 675, 1284
TseI GCWGC 2 cut(s) 83, 122
Tsp45I GTSAC 3 cut(s) 448, 675, 1284
TspDTI ATGAA 8 cut(s) 107, 258, 296, 354, 1368, 1425, 1559, 1575
TspRI CASTG 2 cut(s) 457, 595
Vha464I CTTAAG 1 cut(s) 1399
XapI RAATTY 8 cut(s) 18, 273, 622, 985, 1012, 1192, 1231, 1448
XbaI TCTAGA 1 cut(s) 1342
XceI RCATGY 2 cut(s) 977, 1660
XspI CTAG 4 cut(s) 237, 323, 1343, 1506
ZrmI AGTACT 2 cut(s) 506, 923
Zsp2I ATGCAT 2 cut(s) 661, 1559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.