Rroxscaffold_1G00003900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014307
Physical Location & Seq
Forward (+)
5203994 .. 5206745
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003900.1

Sequence Viewer

Length: 1803 bp
ATGGCTACTGACACCAGAATTTTCCCAGACCAAAACAACAACAACAACAACAACAACATCAAAAACAATGAGATTAACAGGGTGGAAATCCAAGCAGCCATTGCCAAAGCTGTGGAGCTTAGAGCTCTTCATGCTGCTTTGGTTCAAGGAAGCAGCCCTGCAAATCTCAGATTCCCTTCTTCTGCTTCCCCAGCTAATTCACGCCATGCCTCTAACTTCTCTGCTCAGGACTACCCTGTTTTTACACCTAGTTATGAAGACGAACCATCATCCGGGTATCATCAAATTCCAATGAAGAACAGAACGTTATCTGAAAGTTGGGACGAGTATGGGCTAGAAGGAAATGGGGATGAATCAGCTGTGTCAAATTACAAGGAAAACTCATCTTCAAGAAAAGGGTTTCCTTCTGATTTATCCAACTTTGAATCCCACATTTGCCCAGCTGAGGATAACAAGTCTGTCACTGGCTCATGTACAAACAACATCACCGTGCTTCAAACATCACCAGGTACTGAGTACTTCAGGTCAAGTAGGAGAAACAGTTTGGGGGATTTCAACTCAATCTCATCTTGTAATAGATGCAAGCCTGCAACTATAACCAGTGAACCCGAAAACACATCCAGGAACAGCAGGAATTCTAACATTGTTGTGCCATTAACAGATTCTCATGCATCTACTACTACACAGTCACAACCAAAAAGTAGGGGAGTGATTTCTTGGTTGTTTCCGCGGTTAAAGAAGAAGCACAAGAATATAAGCTCTCCAACCCGGACAGAATCTGAGGAAGTTTCTCAAGCCTACAAGGATTTTGGAATCATGTCAATTGAAGTACTGAAGAAACAGCTCATAAAAGCAGATGAGAATAGAGACTCTGCGTTAAAGGAGGTTTCTGAGATGAGATCTTCGCTTGGGGAGCTGAAGCAGAAGCTGGAGTACTTGGAAACTTACTGTGAAGAGCTGAAGAAGGCTTTGAGACAAGCAACACATGCTAATAAGAATTTCCAAATGCCAGAAAATATTGGGAATTTCCCAAACAGACCAGGAAAATCAAGTGATGGGAATGCGGAAAACTTAATGCCGGTCAGCGAGGAAGTAATGGTGGAAGGTTTCCTGCAGATAGTATCCGAAGCAAGACTTTCAGTAAGACAATTCTGCAAAATCCTTGTAGCACAAATTGAACTAACAGATAACACCTTAGTGGACAATTTGAACCTGCTTCTCCAACCATTCAAATTGTCATTGAATTCCAAGTATTCGAAAGCTGTGTCATACCATTTGGAAGCCATCATAAACCAGTCACTTTACCTAGACTTTGAGAACTGTGTGTTCCAGAAGCATGGCTCACCAAAGCTTCTAGATCCTCAACAAGCTCGCCAAGCACAGTTTTCTTCATTCGTGGCACTAAGAAACTTAAGCTGGAATGAAGTTTTGAGGAAGGGAACGAAGTATTACTGTGAGGAATTCAGCAAGTTTTGTGATCAGAAGATGAGTTGCATCATTACAACACTAAATTGGACTAGGCCATGGCCTGAGCAGGTTCTTCAAGCATTCTTTGTAGCTACGAAATGCATTTGGTTACTTCATTTGCTTGCGTTTTCATTCAATCCGCCGCTGGGGATATTGAGGGTGGAAGAGAATAGGAGCTTTGATGCACATTACATGGAAGACATGTTTATGGATAGGCAGAGATCACATGGTTCAAGCAGGGTTAAGATTATGGTAATGCCAGGGTTTTATGTTCAGGATAGGGTTTTAAGGTGTAAGGTTCTTTGCAGGTATAAGTCTGCAGGTCAAGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

600

Amino Acids

67.86

Weight (kDa)

8.42

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIL1_IRKI_C PF24994 540 - 590 2.9e-06 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12900
fragaria_vesca FvH4_3g43680 FvH4_3g43680
malus_domestica MD11G1021700.v1.1
prunus_persica Prupe.6G016100_v2.0.a1
pyrus_communis pycom03g01740 pycom11g01760
rosa_chinensis RchiOBHm_Chr5g0078341
rosa_laevigata RLG00000036768
rosa_roxburghii Rroxscaffold_1G00003900
rosa_rugosa Rorug05G0460200
rosa_samantha Rh5AG514100 Rh5BG537000 Rh5CG560500 Rh5DG548600
rosa_wichuraiana Rw5G047730 Rw5G047870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 5 cut(s) 1221, 1525, 1764, 1778, 1787
AccII CGCG 1 cut(s) 730
AciI CCGC 5 cut(s) 728, 730, 1064, 1607, 1610
AclI AACGTT 1 cut(s) 305
AclWI GGATC 1 cut(s) 1352
AcsI RAATTY 7 cut(s) 18, 285, 634, 997, 1024, 1243, 1460
AcuI CTGAAG 4 cut(s) 505, 854, 938, 980
AfaI GTAC 5 cut(s) 475, 511, 518, 831, 935
AfiI CCNNNNNNNGG 3 cut(s) 272, 445, 1613
AflII CTTAAG 1 cut(s) 1411
AflIII ACRYGT 1 cut(s) 1668
AjnI CCWGG 4 cut(s) 505, 620, 1039, 1726
AleI CACNNNNGTG 1 cut(s) 1196
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 2 cut(s) 861, 967
AlwI GGATC 1 cut(s) 1352
AlwNI CAGNNNCTG 3 cut(s) 512, 779, 928
AoxI GGCC 2 cut(s) 1520, 1526
ApeKI GCWGC 3 cut(s) 95, 134, 153
ApoI RAATTY 7 cut(s) 18, 285, 634, 997, 1024, 1243, 1460
ArsI GACNNNNNNTTYG 2 cut(s) 1250, 1282
AsuC2I CCSGG 2 cut(s) 274, 769
AsuHPI GGTGA 3 cut(s) 478, 495, 1335
AsuII TTCGAA 1 cut(s) 1256
BanII GRGCYC 1 cut(s) 127
BarI GAAGNNNNNNTAC 2 cut(s) 917, 949
BbsI GAAGAC 2 cut(s) 264, 1671
Bbv12I GWGCWC 1 cut(s) 127
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 3 cut(s) 107, 121, 165
BccI CCATC 3 cut(s) 274, 1049, 1292
BciT130I CCWGG 4 cut(s) 507, 622, 1041, 1728
BciVI GTATCC 1 cut(s) 1132
BclI TGATCA 1 cut(s) 1477
BcnI CCSGG 2 cut(s) 274, 769
BcoDI GTCTC 2 cut(s) 861, 967
BfaI CTAG 5 cut(s) 249, 335, 1307, 1355, 1518
BfmI CTRYAG 2 cut(s) 1112, 1785
BfrI CTTAAG 1 cut(s) 1411
BfuAI ACCTGC 5 cut(s) 1221, 1525, 1764, 1778, 1787
BfuI GTATCC 1 cut(s) 1132
BglII AGATCT 1 cut(s) 899
BisI GCNGC 4 cut(s) 96, 135, 154, 1610
BlsI GCNGC 4 cut(s) 97, 136, 155, 1611
BmcAI AGTACT 3 cut(s) 518, 831, 935
Bme1390I CCNGG 6 cut(s) 274, 507, 622, 769, 1041, 1728
BmrFI CCNGG 6 cut(s) 274, 507, 622, 769, 1041, 1728
BmsI GCATC 4 cut(s) 569, 680, 1503, 1639
BpiI GAAGAC 2 cut(s) 264, 1671
BpmI CTGGAG 1 cut(s) 950
Bpu10I CCTNAGC 3 cut(s) 225, 444, 1530
Bpu14I TTCGAA 1 cut(s) 1256
BpuEI CTTGAG 1 cut(s) 777
BpuMI CCSGG 2 cut(s) 274, 769
BsaJI CCNNGG 3 cut(s) 728, 1523, 1727
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 445, 1613
Bse118I RCCGGY 1 cut(s) 1078
Bse1I ACTGG 3 cut(s) 469, 600, 1294
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 4 cut(s) 507, 622, 1041, 1728
BseDI CCNNGG 3 cut(s) 728, 1523, 1727
BseGI GGATG 3 cut(s) 269, 355, 617
BseLI CCNNNNNNNGG 3 cut(s) 272, 445, 1613
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 7 cut(s) 181, 239, 435, 504, 771, 882, 1521
BseNI ACTGG 3 cut(s) 469, 600, 1294
BseXI GCAGC 3 cut(s) 107, 121, 165
BseYI CCCAGC 3 cut(s) 190, 439, 1612
Bsh1236I CGCG 1 cut(s) 730
BshFI GGCC 2 cut(s) 1522, 1528
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 3 cut(s) 273, 769, 1079
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 3 cut(s) 272, 445, 1613
BsmAI GTCTC 2 cut(s) 861, 967
BsmFI GGGAC 1 cut(s) 335
BsmI GAATGC 2 cut(s) 1066, 1547
BsnI GGCC 2 cut(s) 1522, 1528
Bsp119I TTCGAA 1 cut(s) 1256
Bsp1286I GDGCHC 1 cut(s) 127
Bsp1407I TGTACA 1 cut(s) 473
Bsp143I GATC 4 cut(s) 899, 1357, 1477, 1688
Bsp19I CCATGG 1 cut(s) 1523
BspACI CCGC 5 cut(s) 728, 730, 1064, 1607, 1610
BspANI GGCC 2 cut(s) 1522, 1528
BspCNI CTCAG 7 cut(s) 180, 238, 436, 505, 772, 883, 1522
BspFNI CGCG 1 cut(s) 730
BspMAI CTGCAG 2 cut(s) 1116, 1789
BspMI ACCTGC 5 cut(s) 1221, 1525, 1764, 1778, 1787
BspPI GGATC 1 cut(s) 1352
BspQI GCTCTTC 2 cut(s) 132, 948
BspT104I TTCGAA 1 cut(s) 1256
BspTI CTTAAG 1 cut(s) 1411
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 1078
BsrGI TGTACA 1 cut(s) 473
BsrI ACTGG 3 cut(s) 469, 600, 1294
BssAI RCCGGY 1 cut(s) 1078
BssECI CCNNGG 3 cut(s) 728, 1523, 1727
BssMI GATC 4 cut(s) 899, 1357, 1477, 1688
BssT1I CCWWGG 1 cut(s) 1523
Bst2UI CCWGG 4 cut(s) 507, 622, 1041, 1728
Bst4CI ACNGT 7 cut(s) 490, 542, 687, 950, 1322, 1383, 1454
Bst6I CTCTTC 3 cut(s) 132, 948, 1626
BstAFI CTTAAG 1 cut(s) 1411
BstAPI GCANNNNNTGC 2 cut(s) 101, 986
BstAUI TGTACA 1 cut(s) 473
BstBI TTCGAA 1 cut(s) 1256
BstC8I GCNNGC 4 cut(s) 584, 588, 1372, 1590
BstDSI CCRYGG 2 cut(s) 728, 1523
BstF5I GGATG 3 cut(s) 269, 355, 617
BstFNI CGCG 1 cut(s) 730
BstKTI GATC 4 cut(s) 902, 1360, 1480, 1691
BstMAI GTCTC 2 cut(s) 861, 967
BstMBI GATC 4 cut(s) 899, 1357, 1477, 1688
BstMWI GCNNNNNNNGC 7 cut(s) 101, 131, 191, 913, 986, 1376, 1793
BstNI CCWGG 4 cut(s) 507, 622, 1041, 1728
BstNSI RCATGY 2 cut(s) 989, 1672
BstSCI CCNGG 6 cut(s) 272, 505, 620, 767, 1039, 1726
BstSFI CTRYAG 2 cut(s) 1112, 1785
BstUI CGCG 1 cut(s) 730
BstV1I GCAGC 3 cut(s) 107, 121, 165
BstV2I GAAGAC 2 cut(s) 264, 1671
BstX2I RGATCY 2 cut(s) 899, 1357
BstXI CCANNNNNNTGG 2 cut(s) 112, 1337
BstYI RGATCY 2 cut(s) 899, 1357
BsuI GTATCC 1 cut(s) 1132
BsuRI GGCC 2 cut(s) 1522, 1528
BtgI CCRYGG 2 cut(s) 728, 1523
BtsCI GGATG 3 cut(s) 269, 355, 617
BtsIMutI CAGTG 2 cut(s) 462, 607
BveI ACCTGC 5 cut(s) 1221, 1525, 1764, 1778, 1787
Cac8I GCNNGC 4 cut(s) 584, 588, 1372, 1590
CaiI CAGNNNCTG 3 cut(s) 512, 779, 928
Cfr10I RCCGGY 1 cut(s) 1078
Cfr42I CCGCGG 1 cut(s) 731
CsiI ACCWGGT 1 cut(s) 505
Csp6I GTAC 5 cut(s) 474, 510, 517, 830, 934
CviQI GTAC 5 cut(s) 474, 510, 517, 830, 934
DpnI GATC 4 cut(s) 901, 1359, 1479, 1690
DpnII GATC 4 cut(s) 899, 1357, 1477, 1688
Eam1104I CTCTTC 3 cut(s) 132, 948, 1626
EarI CTCTTC 3 cut(s) 132, 948, 1626
EciI GGCGGA 1 cut(s) 1596
Ecl136II GAGCTC 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 1523
Eco24I GRGCYC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 125
Eco57I CTGAAG 4 cut(s) 505, 854, 938, 980
EcoICRI GAGCTC 1 cut(s) 125
EcoRI GAATTC 3 cut(s) 634, 1243, 1460
EcoRII CCWGG 4 cut(s) 505, 620, 1039, 1726
EcoT14I CCWWGG 1 cut(s) 1523
EcoT22I ATGCAT 2 cut(s) 673, 1571
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 1523
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 1 cut(s) 335
FbaI TGATCA 1 cut(s) 1477
Fnu4HI GCNGC 4 cut(s) 96, 135, 154, 1610
FokI GGATG 3 cut(s) 256, 362, 604
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 96, 135, 154, 1610
FspBI CTAG 5 cut(s) 249, 335, 1307, 1355, 1518
GluI GCNGC 4 cut(s) 96, 135, 154, 1610
GsaI CCCAGC 3 cut(s) 194, 443, 1616
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 2 cut(s) 1522, 1528
HapII CCGG 3 cut(s) 273, 769, 1079
HindIII AAGCTT 1 cut(s) 1349
HinfI GANTC 7 cut(s) 171, 353, 425, 662, 776, 813, 869
HpaII CCGG 3 cut(s) 273, 769, 1079
HphI GGTGA 3 cut(s) 478, 495, 1335
Hpy166II GTNNAC 2 cut(s) 605, 1201
Hpy188I TCNGA 7 cut(s) 170, 313, 409, 781, 892, 1126, 1482
Hpy188III TCNNGA 5 cut(s) 227, 390, 1330, 1355, 1742
Hpy8I GTNNAC 2 cut(s) 605, 1201
HpyAV CCTTC 6 cut(s) 186, 332, 414, 958, 1097, 1429
HpyCH4III ACNGT 7 cut(s) 490, 542, 687, 950, 1322, 1383, 1454
HpyCH4IV ACGT 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 131, 191, 913, 986, 1376, 1793
HpySE526I ACGT 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 1477
KspI CCGCGG 1 cut(s) 731
Kzo9I GATC 4 cut(s) 899, 1357, 1477, 1688
LguI GCTCTTC 2 cut(s) 132, 948
LmnI GCTCC 3 cut(s) 115, 913, 1641
Lsp1109I GCAGC 3 cut(s) 107, 121, 165
LweI GCATC 4 cut(s) 569, 680, 1503, 1639
MabI ACCWGGT 1 cut(s) 505
MaeI CTAG 5 cut(s) 249, 335, 1307, 1355, 1518
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 4 cut(s) 460, 687, 1296, 1575
MalI GATC 4 cut(s) 901, 1359, 1479, 1690
MboI GATC 4 cut(s) 899, 1357, 1477, 1688
MfeI CAATTG 1 cut(s) 822
MflI RGATCY 2 cut(s) 899, 1357
MhlI GDGCHC 1 cut(s) 127
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 3 cut(s) 441, 788, 1246
MnlI CCTC 9 cut(s) 220, 439, 775, 877, 1081, 1371, 1425, 1450, 1617
Mph1103I ATGCAT 2 cut(s) 673, 1571
MseI TTAA 8 cut(s) 75, 656, 734, 878, 1073, 1412, 1710, 1754
MslI CAYNNNNRTG 4 cut(s) 488, 647, 1196, 1673
MspA1I CMGCKG 4 cut(s) 359, 443, 730, 1612
MspCI CTTAAG 1 cut(s) 1411
MspI CCGG 3 cut(s) 273, 769, 1079
MspR9I CCNGG 6 cut(s) 274, 507, 622, 769, 1041, 1728
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 2 cut(s) 1066, 1547
MvaI CCWGG 4 cut(s) 507, 622, 1041, 1728
MvnI CGCG 1 cut(s) 730
MwoI GCNNNNNNNGC 7 cut(s) 101, 131, 191, 913, 986, 1376, 1793
NciI CCSGG 2 cut(s) 274, 769
NcoI CCATGG 1 cut(s) 1523
NdeII GATC 4 cut(s) 899, 1357, 1477, 1688
NmuCI GTSAC 3 cut(s) 460, 687, 1296
NsiI ATGCAT 2 cut(s) 673, 1571
NspI RCATGY 2 cut(s) 989, 1672
NspV TTCGAA 1 cut(s) 1256
OliI CACNNNNGTG 1 cut(s) 1196
PciI ACATGT 1 cut(s) 1668
PciSI GCTCTTC 2 cut(s) 132, 948
PctI GAATGC 2 cut(s) 1066, 1547
PfeI GAWTC 6 cut(s) 171, 353, 425, 662, 776, 813
PfoI TCCNGGA 1 cut(s) 620
PkrI GCNGC 4 cut(s) 97, 136, 155, 1611
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
PscI ACATGT 1 cut(s) 1668
Psp124BI GAGCTC 1 cut(s) 127
Psp1406I AACGTT 1 cut(s) 305
Psp6I CCWGG 4 cut(s) 505, 620, 1039, 1726
PspFI CCCAGC 3 cut(s) 190, 439, 1612
PspGI CCWGG 4 cut(s) 505, 620, 1039, 1726
PstI CTGCAG 2 cut(s) 1116, 1789
PstNI CAGNNNCTG 3 cut(s) 512, 779, 928
PsuI RGATCY 2 cut(s) 899, 1357
PvuII CAGCTG 2 cut(s) 359, 443
RsaI GTAC 5 cut(s) 475, 511, 518, 831, 935
RsaNI GTAC 5 cut(s) 474, 510, 517, 830, 934
RseI CAYNNNNRTG 4 cut(s) 488, 647, 1196, 1673
SacI GAGCTC 1 cut(s) 127
SacII CCGCGG 1 cut(s) 731
SapI GCTCTTC 2 cut(s) 132, 948
SaqAI TTAA 8 cut(s) 75, 656, 734, 878, 1073, 1412, 1710, 1754
SatI GCNGC 4 cut(s) 96, 135, 154, 1610
Sau3AI GATC 4 cut(s) 899, 1357, 1477, 1688
ScaI AGTACT 3 cut(s) 518, 831, 935
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 6 cut(s) 274, 507, 622, 769, 1041, 1728
SduI GDGCHC 1 cut(s) 127
SexAI ACCWGGT 1 cut(s) 505
SfaNI GCATC 4 cut(s) 569, 680, 1503, 1639
SfcI CTRYAG 2 cut(s) 1112, 1785
Sfr303I CCGCGG 1 cut(s) 731
SfuI TTCGAA 1 cut(s) 1256
SgrBI CCGCGG 1 cut(s) 731
SmiMI CAYNNNNRTG 4 cut(s) 488, 647, 1196, 1673
SmlI CTYRAG 2 cut(s) 792, 1411
SmoI CTYRAG 2 cut(s) 792, 1411
SsiI CCGC 5 cut(s) 728, 730, 1064, 1607, 1610
SspI AATATT 1 cut(s) 1018
SspMI CTAG 5 cut(s) 249, 335, 1307, 1355, 1518
SstI GAGCTC 1 cut(s) 127
StyD4I CCNGG 6 cut(s) 272, 505, 620, 767, 1039, 1726
StyI CCWWGG 1 cut(s) 1523
TaaI ACNGT 7 cut(s) 490, 542, 687, 950, 1322, 1383, 1454
TaiI ACGT 1 cut(s) 308
TaqI TCGA 1 cut(s) 1256
TatI WGTACW 4 cut(s) 473, 516, 829, 933
TauI GCSGC 1 cut(s) 1612
TfiI GAWTC 6 cut(s) 171, 353, 425, 662, 776, 813
Tru1I TTAA 8 cut(s) 75, 656, 734, 878, 1073, 1412, 1710, 1754
Tru9I TTAA 8 cut(s) 75, 656, 734, 878, 1073, 1412, 1710, 1754
TscAI CASTG 2 cut(s) 469, 607
TseFI GTSAC 3 cut(s) 460, 687, 1296
TseI GCWGC 3 cut(s) 95, 134, 153
Tsp45I GTSAC 3 cut(s) 460, 687, 1296
TspDTI ATGAA 8 cut(s) 119, 270, 308, 366, 1380, 1437, 1571, 1587
TspRI CASTG 2 cut(s) 469, 607
Vha464I CTTAAG 1 cut(s) 1411
XapI RAATTY 7 cut(s) 18, 285, 634, 997, 1024, 1243, 1460
XbaI TCTAGA 1 cut(s) 1354
XceI RCATGY 2 cut(s) 989, 1672
XspI CTAG 5 cut(s) 249, 335, 1307, 1355, 1518
ZrmI AGTACT 3 cut(s) 518, 831, 935
Zsp2I ATGCAT 2 cut(s) 673, 1571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.