AT5G15460

May serve as docking site to facilitate the association of other proteins to the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005273
Physical Location & Seq
Reverse (-)
5018680 .. 5020926
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15460.2

Sequence Viewer

Length: 375 bp
ATGGCAGAGGTGAAGGATCAATTAGAGATTAAGTTCCGGCTTAACGATGGTTCTGATATCGGTCCTAAATTGTTTCCTGATGCTACTACCGTTGCTACATTGAAAGAAACCGTTGTTGCTCAGTGGCCAAGAGATAAGGAGAACGGGCCAAAGACAGTGAAAGATGTTAAACTGATAAGCGCGGGTAGAATATTGGAGAACAACAAAACGGTTGGAGATTGCAGGAGTCCCGTCGGCAATTTCTCAGGTGCTGTCACCACAATGCATGTTATAATTCAACATCAAGTTACTGAAAAAGAAAAGAAGAAGAAGAAGCCTAAAGGTGATCTGAAACAGAACAAATGTGTCTGTTTATGTTTTGGAGCTCGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

124

Amino Acids

13.71

Weight (kDa)

9.38

Isoelectric Point (pI)

21.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 6 - 117 2e-46 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AccII CGCG 1 cut(s) 182
AciI CCGC 1 cut(s) 182
AclWI GGATC 1 cut(s) 24
AcoI YGGCCR 1 cut(s) 125
AgsI TTSAA 2 cut(s) 103, 278
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
Alw21I GWGCWC 1 cut(s) 367
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 2 cut(s) 125, 146
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 2 cut(s) 62, 146
AsuHPI GGTGA 3 cut(s) 22, 247, 335
AvaII GGWCC 1 cut(s) 62
BalI TGGCCA 1 cut(s) 127
BanII GRGCYC 1 cut(s) 367
Bbv12I GWGCWC 1 cut(s) 367
BccI CCATC 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 2 cut(s) 62, 146
BmsI GCATC 1 cut(s) 70
BseMII CTCAG 2 cut(s) 134, 258
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 127, 148
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 37
BslFI GGGAC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 2 cut(s) 127, 148
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 2 cut(s) 16, 325
BspACI CCGC 1 cut(s) 182
BspANI GGCC 2 cut(s) 127, 148
BspCNI CTCAG 2 cut(s) 133, 257
BspFNI CGCG 1 cut(s) 182
BspPI GGATC 1 cut(s) 24
BssMI GATC 2 cut(s) 16, 325
Bst4CI ACNGT 4 cut(s) 91, 112, 157, 211
BstDEI CTNAG 2 cut(s) 120, 244
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 19, 328
BstMBI GATC 2 cut(s) 16, 325
BstNSI RCATGY 1 cut(s) 269
BstUI CGCG 1 cut(s) 182
BsuRI GGCC 2 cut(s) 127, 148
BtsIMutI CAGTG 2 cut(s) 128, 162
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 62, 146
CviAII CATG 1 cut(s) 266
CviJI RGCY 5 cut(s) 40, 127, 148, 316, 365
CviKI_1 RGCY 5 cut(s) 40, 127, 148, 316, 365
DdeI CTNAG 2 cut(s) 120, 244
DpnI GATC 2 cut(s) 18, 327
DpnII GATC 2 cut(s) 16, 325
EaeI YGGCCR 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 367
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 365
EcoRV GATATC 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 367
FaeI CATG 1 cut(s) 269
FaiI YATR 3 cut(s) 267, 272, 355
FaqI GGGAC 1 cut(s) 213
FatI CATG 1 cut(s) 265
FauI CCCGC 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 367
GlaI GCGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 127, 148
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 182
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 1 cut(s) 226
HpaII CCGG 1 cut(s) 37
HphI GGTGA 3 cut(s) 22, 247, 335
Hpy188I TCNGA 2 cut(s) 55, 330
Hpy188III TCNNGA 1 cut(s) 77
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 4 cut(s) 91, 112, 157, 211
HpyCH4V TGCA 2 cut(s) 222, 265
HpyF3I CTNAG 2 cut(s) 120, 244
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 2 cut(s) 16, 325
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 4 cut(s) 50, 90, 208, 231
LweI GCATC 1 cut(s) 70
MaeIII GTNAC 2 cut(s) 253, 286
MalI GATC 2 cut(s) 18, 327
MboI GATC 2 cut(s) 16, 325
MboII GAAGA 3 cut(s) 316, 319, 322
MhlI GDGCHC 1 cut(s) 367
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 4 cut(s) 20, 68, 238, 273
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 1 cut(s) 193
Mox20I TGGCCA 1 cut(s) 127
Mph1103I ATGCAT 1 cut(s) 267
MscI TGGCCA 1 cut(s) 127
MseI TTAA 4 cut(s) 30, 42, 168, 373
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 127
MspI CCGG 1 cut(s) 37
MvnI CGCG 1 cut(s) 182
NdeII GATC 2 cut(s) 16, 325
NlaIII CATG 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 269
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
PsiI TTATAA 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 367
PspPI GGNCC 2 cut(s) 62, 146
PstNI CAGNNNCTG 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 367
SaqAI TTAA 4 cut(s) 30, 42, 168, 373
Sau3AI GATC 2 cut(s) 16, 325
Sau96I GGNCC 2 cut(s) 62, 146
SchI GAGTC 1 cut(s) 235
SduI GDGCHC 1 cut(s) 367
SetI ASST 4 cut(s) 12, 250, 325, 367
SfaNI GCATC 1 cut(s) 70
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 4 cut(s) 20, 68, 238, 273
SsiI CCGC 1 cut(s) 182
SspI AATATT 1 cut(s) 192
SstI GAGCTC 1 cut(s) 367
TaaI ACNGT 4 cut(s) 91, 112, 157, 211
TaqII GACCGA 1 cut(s) 50
TasI AATT 4 cut(s) 20, 68, 238, 273
Tru1I TTAA 4 cut(s) 30, 42, 168, 373
Tru9I TTAA 4 cut(s) 30, 42, 168, 373
TscAI CASTG 2 cut(s) 128, 162
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
TspRI CASTG 2 cut(s) 128, 162
VpaK11BI GGWCC 1 cut(s) 62
XceI RCATGY 1 cut(s) 269
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.