AT5G16250

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015372
Physical Location & Seq
Reverse (-)
5310052 .. 5310951
900 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16250.1

Sequence Viewer

Length: 552 bp
ATGGGTTTCATTTCTTCTTCATCACCAGTAGAAGAGTCTCATTACCATACACACAAGATCTTCCTTTTCTCCAACTACATCCTTCTCGGCGCTGCTTCAAGTTGTATCTTCCTCACACTCTCTCTCCGTCTAATCCCTTCCATCTGCGGATTCCTCCTCATCCTCCTCCACGCCACCACAATCGCCGCCGCAGTCTCAGGCTGCGCTGCTGCATCTTGCGGAAGAAACCGTTGGTACGCCGCTCACATGGTTGCAACTGTGCTCACTGCTATATTCCAAGGCTCTGTCTCTGTTCTCATCTTCACCAACACGTCTAAATTCCTCGGGAGTCTTAAGTCGTATGTGCGTGAGGAAGATGCTGCTGTGATTTTGAAACTCGGTGGTGGTCTTTGTATTGTGATCTTTTGTCTTGATTGGATCGTTCTTGTGTGTGCTTTCTTCTTGAAGTACTATGCTTATGTCGATGGTGGTGATGGCGTTGCGATGAAGAGAACTGGTAAGGTTCAGAGTGAGGAGAATCCGAAGGACTGGCCATGGCCGTTCCAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

19.96

Weight (kDa)

8.16

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16250
fragaria_vesca FvH4_6g00070
malus_domestica MD00G1017200.v1.1 MD12G1261500.v1.1
prunus_persica Prupe.6G365300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0447401
rosa_laevigata RLG00000026002
rosa_multiflora Rmu_ssc0000473.1_g000017 Rmu_ssc0000473.1_g000019
rosa_roxburghii Rroxscaffold_6G00425600
rosa_rugosa Rorug02G0601200
rosa_samantha Rh3AG001300 Rh3BG001000 Rh3CG001100 Rh3DG001200
rosa_wichuraiana Rw3G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 242
AciI CCGC 5 cut(s) 147, 186, 189, 219, 240
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 2 cut(s) 530, 536
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 2 cut(s) 236, 449
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 3 cut(s) 99, 373, 445
AjiI CACGTC 1 cut(s) 312
AjuI GAANNNNNNNTTGG 2 cut(s) 214, 246
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 3 cut(s) 42, 199, 292
AlwI GGATC 1 cut(s) 425
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 2 cut(s) 530, 536
ApeKI GCWGC 5 cut(s) 92, 201, 206, 209, 359
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 2 cut(s) 92, 206
AsuHPI GGTGA 3 cut(s) 15, 295, 482
AvaI CYCGRG 1 cut(s) 323
BalI TGGCCA 1 cut(s) 532
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 5 cut(s) 79, 188, 193, 196, 346
BccI CCATC 3 cut(s) 149, 458, 467
BceAI ACGGC 1 cut(s) 523
BcoDI GTCTC 3 cut(s) 42, 199, 292
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 332
BglII AGATCT 1 cut(s) 57
BisI GCNGC 8 cut(s) 93, 186, 189, 202, 207, 210, 240, 360
BlsI GCNGC 8 cut(s) 94, 187, 190, 203, 208, 211, 241, 361
BmcAI AGTACT 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 312
BmsI GCATC 2 cut(s) 221, 346
BsaJI CCNNGG 3 cut(s) 277, 322, 533
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bse1I ACTGG 3 cut(s) 26, 499, 533
BseDI CCNNGG 3 cut(s) 277, 322, 533
BseGI GGATG 2 cut(s) 78, 159
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 3 cut(s) 26, 499, 533
BseRI GAGGAG 3 cut(s) 146, 155, 527
BseXI GCAGC 5 cut(s) 79, 188, 193, 196, 346
BshFI GGCC 2 cut(s) 532, 538
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 323
BsmAI GTCTC 3 cut(s) 42, 199, 292
BsnI GGCC 2 cut(s) 532, 538
BsoBI CYCGRG 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 3 cut(s) 57, 399, 417
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 5 cut(s) 147, 186, 189, 219, 240
BspANI GGCC 2 cut(s) 532, 538
BspCNI CTCAG 1 cut(s) 209
BspPI GGATC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 242
BsrI ACTGG 3 cut(s) 26, 499, 533
BssECI CCNNGG 3 cut(s) 277, 322, 533
BssMI GATC 3 cut(s) 57, 399, 417
BssT1I CCWWGG 2 cut(s) 277, 533
Bst4CI ACNGT 2 cut(s) 230, 259
Bst6I CTCTTC 2 cut(s) 27, 482
BstAFI CTTAAG 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 196
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 2 cut(s) 78, 159
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 206
BstKTI GATC 3 cut(s) 60, 402, 420
BstMAI GTCTC 3 cut(s) 42, 199, 292
BstMBI GATC 3 cut(s) 57, 399, 417
BstV1I GCAGC 5 cut(s) 79, 188, 193, 196, 346
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
BsuRI GGCC 2 cut(s) 532, 538
BtgI CCRYGG 1 cut(s) 533
BtgZI GCGATG 1 cut(s) 497
BtrI CACGTC 1 cut(s) 312
BtsCI GGATG 2 cut(s) 78, 159
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 264
CfoI GCGC 2 cut(s) 92, 206
Csp6I GTAC 2 cut(s) 235, 448
CviAII CATG 2 cut(s) 247, 534
CviJI RGCY 4 cut(s) 201, 282, 532, 538
CviKI_1 RGCY 4 cut(s) 201, 282, 532, 538
CviQI GTAC 2 cut(s) 235, 448
DdeI CTNAG 1 cut(s) 196
DpnI GATC 3 cut(s) 59, 401, 419
DpnII GATC 3 cut(s) 57, 399, 417
EaeI YGGCCR 2 cut(s) 530, 536
Eam1104I CTCTTC 2 cut(s) 27, 482
EarI CTCTTC 2 cut(s) 27, 482
Eco130I CCWWGG 2 cut(s) 277, 533
Eco88I CYCGRG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 277, 533
ErhI CCWWGG 2 cut(s) 277, 533
FaeI CATG 2 cut(s) 250, 537
FaiI YATR 7 cut(s) 48, 248, 272, 342, 453, 459, 535
FatI CATG 2 cut(s) 246, 533
Fnu4HI GCNGC 8 cut(s) 93, 186, 189, 202, 207, 210, 240, 360
FokI GGATG 2 cut(s) 65, 146
Fsp4HI GCNGC 8 cut(s) 93, 186, 189, 202, 207, 210, 240, 360
GlaI GCGC 2 cut(s) 91, 205
GluI GCNGC 8 cut(s) 93, 186, 189, 202, 207, 210, 240, 360
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 532, 538
HhaI GCGC 2 cut(s) 92, 206
Hin1II CATG 2 cut(s) 250, 537
Hin6I GCGC 2 cut(s) 90, 204
HinP1I GCGC 2 cut(s) 90, 204
HinfI GANTC 4 cut(s) 35, 150, 328, 517
HphI GGTGA 3 cut(s) 15, 295, 482
Hpy188I TCNGA 2 cut(s) 507, 522
Hpy188III TCNNGA 3 cut(s) 325, 410, 442
HpyAV CCTTC 3 cut(s) 92, 147, 517
HpyCH4III ACNGT 2 cut(s) 230, 259
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 2 cut(s) 212, 254
HpyF3I CTNAG 1 cut(s) 196
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 2 cut(s) 250, 537
HspAI GCGC 2 cut(s) 90, 204
Kzo9I GATC 3 cut(s) 57, 399, 417
LpnPI CCDG 4 cut(s) 39, 183, 480, 514
Lsp1109I GCAGC 5 cut(s) 79, 188, 193, 196, 346
LweI GCATC 2 cut(s) 221, 346
MaeII ACGT 1 cut(s) 311
MalI GATC 3 cut(s) 59, 401, 419
MbiI CCGCTC 1 cut(s) 242
MboI GATC 3 cut(s) 57, 399, 417
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 264
MlsI TGGCCA 1 cut(s) 532
MluCI AATT 1 cut(s) 317
MluNI TGGCCA 1 cut(s) 532
MlyI GAGTC 2 cut(s) 44, 337
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 8 cut(s) 122, 164, 167, 173, 176, 332, 343, 505
Mox20I TGGCCA 1 cut(s) 532
MscI TGGCCA 1 cut(s) 532
MseI TTAA 2 cut(s) 333, 550
Msp20I TGGCCA 1 cut(s) 532
MspCI CTTAAG 1 cut(s) 332
NcoI CCATGG 1 cut(s) 533
NdeII GATC 3 cut(s) 57, 399, 417
NlaIII CATG 2 cut(s) 250, 537
NmeAIII GCCGAG 1 cut(s) 66
PfeI GAWTC 2 cut(s) 150, 517
PkrI GCNGC 8 cut(s) 94, 187, 190, 203, 208, 211, 241, 361
PleI GAGTC 2 cut(s) 43, 336
PpsI GAGTC 2 cut(s) 43, 336
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 2 cut(s) 236, 449
RsaNI GTAC 2 cut(s) 235, 448
SaqAI TTAA 2 cut(s) 333, 550
SatI GCNGC 8 cut(s) 93, 186, 189, 202, 207, 210, 240, 360
Sau3AI GATC 3 cut(s) 57, 399, 417
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 2 cut(s) 44, 337
SduI GDGCHC 1 cut(s) 264
SetI ASST 2 cut(s) 314, 504
SfaNI GCATC 2 cut(s) 221, 346
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 1 cut(s) 317
SsiI CCGC 5 cut(s) 147, 186, 189, 219, 240
StyI CCWWGG 2 cut(s) 277, 533
TaaI ACNGT 2 cut(s) 230, 259
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 462
TasI AATT 1 cut(s) 317
TatI WGTACW 1 cut(s) 447
TauI GCSGC 3 cut(s) 188, 191, 242
TfiI GAWTC 2 cut(s) 150, 517
Tru1I TTAA 2 cut(s) 333, 550
Tru9I TTAA 2 cut(s) 333, 550
TscAI CASTG 1 cut(s) 271
TseI GCWGC 5 cut(s) 92, 201, 206, 209, 359
TspDTI ATGAA 2 cut(s) 9, 500
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 1 cut(s) 271
Vha464I CTTAAG 1 cut(s) 332
XapI RAATTY 1 cut(s) 317
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.