MD12G1261500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015372
Physical Location & Seq
Forward (+)
32832472 .. 32833096
625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1261500.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGGTGGCATGTCGATGAATGTGGCGAAGTTGGGAGGAGCAAGGTCAGAGTCGGCATACCACACCCACAAGGTGTTCCTGCTGAGCAACTACATATTGCTGGGAGCGGCCTCCAGCTGCATCTTCCTGACGCTGTCTCTACGGCTGCTGCCGTCGGTGTGTGGCTTCTTCTTGATCCTGCTCCACGTGCTTACAATTGCCGGGGCCATATCGGGATGCGCGGCGGCGGGGAGCAGGTCGAGCTCCAAGTGGTACGGAGCGCACATGGTGTCAACGGTGCTGACGGCGATATTCCAAGGGTCGGTGGCGGTTCTGGTGTTCACGAGGACGGGGGACTTTCTCGGGGAGCTCAAGTCGTACGTGAGGGAAGAGGATGGGAGTGTGATTCTGAAATTGGCAGGAGGGCTGAGCGTGGTGATATTCTGCTTGGAGTGGGTGGTGCTCACACTCGCCTTCTTCCTGAGGTACTATGCCTCTGTTGAGGGAAGTGGTGGTGCTGGTGCTGGTGCTGGTATGAGGAGTTCCAAGGTGCAACAAGCAGCTGACGAGGATTTGAAGGACTGGCCATGGCCTTTCCAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

20.58

Weight (kDa)

8.37

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16250
fragaria_vesca FvH4_6g00070
malus_domestica MD00G1017200.v1.1 MD12G1261500.v1.1
prunus_persica Prupe.6G365300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0447401
rosa_laevigata RLG00000026002
rosa_multiflora Rmu_ssc0000473.1_g000017 Rmu_ssc0000473.1_g000019
rosa_roxburghii Rroxscaffold_6G00425600
rosa_rugosa Rorug02G0601200
rosa_samantha Rh3AG001300 Rh3BG001000 Rh3CG001100 Rh3DG001200
rosa_wichuraiana Rw3G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 107
AccII CGCG 1 cut(s) 221
AciI CCGC 5 cut(s) 107, 221, 224, 227, 308
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 1 cut(s) 563
AcvI CACGTG 1 cut(s) 187
AdeI CACNNNGTG 2 cut(s) 73, 268
AfaI GTAC 3 cut(s) 254, 359, 467
AfiI CCNNNNNNNGG 1 cut(s) 301
AgsI TTSAA 1 cut(s) 556
AluBI AGCT 4 cut(s) 117, 243, 349, 542
AluI AGCT 4 cut(s) 117, 243, 349, 542
Alw21I GWGCWC 3 cut(s) 245, 351, 444
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 1 cut(s) 169
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 4 cut(s) 108, 204, 563, 569
ApeKI GCWGC 4 cut(s) 117, 145, 148, 539
AspLEI GCGC 2 cut(s) 221, 262
AspS9I GGNCC 1 cut(s) 204
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 341
AxyI CCTNAGG 1 cut(s) 461
BalI TGGCCA 1 cut(s) 565
BanII GRGCYC 2 cut(s) 245, 351
BauI CACGAG 1 cut(s) 322
BbrPI CACGTG 1 cut(s) 187
Bbv12I GWGCWC 3 cut(s) 245, 351, 444
BbvI GCAGC 4 cut(s) 104, 132, 135, 551
BccI CCATC 1 cut(s) 368
BceAI ACGGC 3 cut(s) 136, 158, 300
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 225
BisI GCNGC 7 cut(s) 108, 118, 146, 149, 222, 225, 540
BlpI GCTNAGC 2 cut(s) 83, 407
BlsI GCNGC 7 cut(s) 109, 119, 147, 150, 223, 226, 541
Bme1390I CCNGG 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 2 cut(s) 129, 206
BpmI CTGGAG 1 cut(s) 97
Bpu1102I GCTNAGC 2 cut(s) 83, 407
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 2 cut(s) 187, 361
BsaJI CCNNGG 4 cut(s) 201, 295, 525, 566
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 566
Bse21I CCTNAGG 1 cut(s) 461
BseDI CCNNGG 4 cut(s) 201, 295, 525, 566
BseGI GGATG 2 cut(s) 221, 379
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMII CTCAG 3 cut(s) 74, 398, 452
BseNI ACTGG 1 cut(s) 566
BseRI GAGGAG 2 cut(s) 51, 532
BseXI GCAGC 4 cut(s) 104, 132, 135, 551
BseYI CCCAGC 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 4 cut(s) 110, 206, 565, 571
BsiHKAI GWGCWC 3 cut(s) 245, 351, 444
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 1 cut(s) 201
BsiWI CGTACG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 301
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 4 cut(s) 110, 206, 565, 571
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 3 cut(s) 245, 351, 444
Bsp143I GATC 1 cut(s) 174
Bsp1720I GCTNAGC 2 cut(s) 83, 407
Bsp19I CCATGG 1 cut(s) 566
BspACI CCGC 5 cut(s) 107, 221, 224, 227, 308
BspANI GGCC 4 cut(s) 110, 206, 565, 571
BspCNI CTCAG 3 cut(s) 75, 399, 453
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 225
BspPI GGATC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 566
BssECI CCNNGG 4 cut(s) 201, 295, 525, 566
BssMI GATC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 322
BssT1I CCWWGG 3 cut(s) 295, 525, 566
Bst2BI CACGAG 1 cut(s) 322
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 363
BstBAI YACGTR 2 cut(s) 187, 361
BstDEI CTNAG 3 cut(s) 83, 407, 461
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 2 cut(s) 221, 379
BstFNI CGCG 1 cut(s) 221
BstHHI GCGC 2 cut(s) 221, 262
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 187, 240
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 200
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 4 cut(s) 104, 132, 135, 551
Bsu36I CCTNAGG 1 cut(s) 461
BsuRI GGCC 4 cut(s) 110, 206, 565, 571
BtgI CCRYGG 1 cut(s) 566
BtsCI GGATG 2 cut(s) 221, 379
BveI ACCTGC 1 cut(s) 225
CfoI GCGC 2 cut(s) 221, 262
Cfr13I GGNCC 1 cut(s) 204
CseI GACGC 1 cut(s) 139
Csp6I GTAC 3 cut(s) 253, 358, 466
CviAII CATG 3 cut(s) 10, 265, 567
CviQI GTAC 3 cut(s) 253, 358, 466
DdeI CTNAG 3 cut(s) 83, 407, 461
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
DraIII CACNNNGTG 2 cut(s) 73, 268
EaeI YGGCCR 1 cut(s) 563
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Ecl136II GAGCTC 2 cut(s) 243, 349
Eco130I CCWWGG 3 cut(s) 295, 525, 566
Eco24I GRGCYC 2 cut(s) 245, 351
Eco53kI GAGCTC 2 cut(s) 243, 349
Eco72I CACGTG 1 cut(s) 187
Eco81I CCTNAGG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 2 cut(s) 243, 349
EcoT14I CCWWGG 3 cut(s) 295, 525, 566
EcoT38I GRGCYC 2 cut(s) 245, 351
ErhI CCWWGG 3 cut(s) 295, 525, 566
FaeI CATG 3 cut(s) 13, 268, 570
FaiI YATR 8 cut(s) 11, 58, 95, 209, 266, 471, 515, 568
FaqI GGGAC 1 cut(s) 347
FatI CATG 3 cut(s) 9, 264, 566
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 7 cut(s) 108, 118, 146, 149, 222, 225, 540
FokI GGATG 2 cut(s) 228, 386
FriOI GRGCYC 2 cut(s) 245, 351
Fsp4HI GCNGC 7 cut(s) 108, 118, 146, 149, 222, 225, 540
GlaI GCGC 2 cut(s) 220, 261
GluI GCNGC 7 cut(s) 108, 118, 146, 149, 222, 225, 540
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 4 cut(s) 110, 206, 565, 571
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 139
HhaI GCGC 2 cut(s) 221, 262
Hin1II CATG 3 cut(s) 13, 268, 570
Hin6I GCGC 2 cut(s) 219, 260
HinP1I GCGC 2 cut(s) 219, 260
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 2 cut(s) 50, 385
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 2 cut(s) 273, 321
Hpy188I TCNGA 3 cut(s) 49, 390, 584
Hpy188III TCNNGA 5 cut(s) 127, 172, 213, 322, 460
Hpy8I GTNNAC 2 cut(s) 273, 321
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 463, 550
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 2 cut(s) 186, 360
HpyCH4V TGCA 2 cut(s) 120, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 240
HpyF3I CTNAG 3 cut(s) 83, 407, 461
HpySE526I ACGT 2 cut(s) 186, 360
Hsp92II CATG 3 cut(s) 13, 268, 570
HspAI GCGC 2 cut(s) 219, 260
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 7 cut(s) 38, 104, 186, 231, 248, 257, 346
Lsp1109I GCAGC 4 cut(s) 104, 132, 135, 551
LweI GCATC 2 cut(s) 129, 206
MaeII ACGT 2 cut(s) 186, 360
MalI GATC 1 cut(s) 176
MbiI CCGCTC 1 cut(s) 107
MboI GATC 1 cut(s) 174
MboII GAAGA 4 cut(s) 115, 160, 380, 448
MfeI CAATTG 1 cut(s) 195
MhlI GDGCHC 3 cut(s) 245, 351, 444
MlsI TGGCCA 1 cut(s) 565
MluCI AATT 2 cut(s) 195, 392
MluNI TGGCCA 1 cut(s) 565
MlyI GAGTC 1 cut(s) 59
Mox20I TGGCCA 1 cut(s) 565
MscI TGGCCA 1 cut(s) 565
MslI CAYNNNNRTG 1 cut(s) 14
Msp20I TGGCCA 1 cut(s) 565
MspA1I CMGCKG 2 cut(s) 117, 542
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
MunI CAATTG 1 cut(s) 195
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 187, 240
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 566
NdeII GATC 1 cut(s) 174
NlaIII CATG 3 cut(s) 13, 268, 570
NlaIV GGNNCC 1 cut(s) 205
NspI RCATGY 1 cut(s) 13
PfeI GAWTC 1 cut(s) 385
Pfl23II CGTACG 1 cut(s) 357
PflFI GACNNNGTC 1 cut(s) 133
PkrI GCNGC 7 cut(s) 109, 119, 147, 150, 223, 226, 541
PleI GAGTC 1 cut(s) 58
PmaCI CACGTG 1 cut(s) 187
PmlI CACGTG 1 cut(s) 187
PpsI GAGTC 1 cut(s) 58
Ppu21I YACGTR 2 cut(s) 187, 361
Psp124BI GAGCTC 2 cut(s) 245, 351
PspCI CACGTG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 100
PspLI CGTACG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 204
PsrI GAACNNNNNNTAC 2 cut(s) 505, 537
PsyI GACNNNGTC 1 cut(s) 133
PvuII CAGCTG 2 cut(s) 117, 542
RsaI GTAC 3 cut(s) 254, 359, 467
RsaNI GTAC 3 cut(s) 253, 358, 466
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 2 cut(s) 245, 351
SatI GCNGC 7 cut(s) 108, 118, 146, 149, 222, 225, 540
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 3 cut(s) 245, 351, 444
SfaNI GCATC 2 cut(s) 129, 206
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 2 cut(s) 195, 392
SsiI CCGC 5 cut(s) 107, 221, 224, 227, 308
SstI GAGCTC 2 cut(s) 245, 351
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 3 cut(s) 295, 525, 566
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 2 cut(s) 189, 363
TaqI TCGA 2 cut(s) 14, 239
TasI AATT 2 cut(s) 195, 392
TauI GCSGC 3 cut(s) 110, 224, 227
TfiI GAWTC 1 cut(s) 385
TseI GCWGC 4 cut(s) 117, 145, 148, 539
TspDTI ATGAA 1 cut(s) 32
TspGWI ACGGA 1 cut(s) 270
Tth111I GACNNNGTC 1 cut(s) 133
XceI RCATGY 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.