AT5G16280

ER-Golgi trafficking TRAPP I complex 85 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010066
Physical Location & Seq
Reverse (-)
5323133 .. 5331694
8562 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16280.2

Sequence Viewer

Length: 3819 bp
ATGGTGGAGCCGGTGAACTCTTCGCTTGGGAAAATGCTATTGGAGGAAATTAGTCCAGTGGTCATGGTTCTATGTACGCCTCTTGTTGAAGATACTTTCCTCAGGAATGGCTTATCCTTTGTGGAAACGCTCAAGCCATTCTGCAACTTTAGTAACATCGATGTGCCTGTTCGGACATCGAGCGATCAGCTGTATCGGCTAAAGAAGTTTACATTAAGATTGTTTTATGCCTCCGATATAAAGCAACCAAACGTAGAGGTTGCTAAGCAACGGCTAGAACGTGTTATTACTCAAGCTGGGGAGAAGGATTTTCAGGATTTGAAATCTGACCCTCCTCAAATTACTGATATACTCTCTAATCCAGAGTCTGAAATTGCACCCTCATGGTTTCGGTATTACAATAAAGAACTTATACGTACATTGTCGTTTTCAGACCACGAAGCTTTCGATCATCCTGTGGCATGTCTATTGGTCGTTTCATCAAAAGATGAAGAACCTATAAAAAAATTTGTAGATCTTTTCAACTCCAATAGATTACCTACTGATGGTACAATGGACCCAAACATTTTGAAGCATTACTTGTTGGTGCATGACAATCAAGATGCAACAACAGAGAGAACGTCTAAAGTTCTGTCAGAGATGAGAAGTCAATTTGGAAATAATGAATGCAACTTACTTTGCACTAATTCATCTAAAGAGGGGAACGTGGAACATCAGGCTAATCCCTGGGCTTCATTTAAATCAAGTGTTTCGGCCGACAAACTTGGATGTGCTCTCACTGGTGATGATATTGTGGAGATCAAAGATCTGATGCAAGAGTTTGCTTCAAGGCATATAATTCCTTACATGGAGCAGAAAGTTCGAGAACTTAATCAACAGATTTCTGCAACAAGGAAAGGTCTTAAAAATCAATTTAAAAACTTTTTGTGGAGAAAAGGAAAAGACGATAATCCGGATGCCACCAAAGGTTCTATGTATACATATAGCTCGACCGAATCTCAAATTAGAATTTTGGGTGACTACGCCTTCATGTTGCATGATTATGAACTTGCATTGTCAAGCTATCGGTTAATATATACAGACTATAATATCGATAAAGCTTGGAAACACTATGCTGGTGTTCAGGAAATGAGGGGACTTGCATATTTCATCTCAGACCAGCCAAAAAAGGATTCTGAGTGCATGGAAAATGCATTCAGCACTTATATGAAACTTGGGAAGTCTGGCTTCCAAAATGCTACGAGATGTGGGCTTTGGTGGGCAGAGATGCTAAAGGCTAGAGATCAGCACAAAGAAGCTGCTTCGGTTTACTTCCGCATATGTGGAGAGGAACCCTTGCACGCTGCAGTCATGTTGGAGCAAGCTTCTTACTGTTTCATGTTAACCAAGCCAGCGATGTTACACAAGTATGGATTTCATCTAGTTCTCTCAGGAGACCACTATAAAAACTGTAATCAGGTTAACCATGCAATTCGTACATACAAAAGTGCAATCTCTGTTTATAAATCAACTACATGGAGCCATATCAAAGATCATTTATATTTTCATATTGGACAGTGGTATGCAATTGTTGGGATGCATGATGTTGCAGTTAGAAACATGCTCAAAGTATTGGACTGTGGCTACCAATCCAAGGCAACCCAAGAGATTTTTCTCAGGGACTTTTTCGACATTGTTAAGAAAACTGGAATGAAGCATGAGGTGGTGGGACTTCAACTACCAATTCTTAATATGTCGTCTCTTCAAGTTATATATGAAGACCATCGCACTTATGCATCTCAAGCTTCTGCTCTTGTAGAAGAGAGCATCTGGCAATCACTGGAGGATGATATCATCCCATCATTAAATTCTGGCAAGTCAAACTGGCTGGAATTACAGTCAAAGCTTCTGCCAAAGAAATACAAGGAATCAAATGTTTGTGTCGCTGGAGAGTCGGTGAAATTGGATCTGGAGTTTAGGAATCCATTGCTAATCTCTACTTCCATAACAAGTGTGTCTCTTATCTGTGAACTTACCGCAAATTCTGACGACCTAAAATTAGTAGATAATGAGCCAAGTGGCTCAAGCTTGAGTCCTGAGATTTCCACAGAACATAATCAGGTTACAACCTCTGGTTTTTCTTCTTTCACTTTGTCTGAAGTGGACTTCACATTAGGTGGAGGCGAGAAAAAATTGGTGAGACTCACTGTTACCCCCAGTGAAGAAGGTATCCTCAAAATTGTCGGTGTAAGGTGGGAGTTGTCTGGTTCCATCGTAGGTGTGCATTACTTCCAATCTGTGTCTGTCAAGGCAAAAACTGCGAGAGGAAGAAGGAAAAACAAACTTACACCCACTGATGCCTTGAAATTTTTAGTCATCAAGAGTCTACCCAGGCTTGAGGGTTCAATTGATCATCTGCCTGAGAAATTGTATGCTGGAGATTTACGTTATCTTGTTTTAGAGTTAAGAAATAAGTCAGAATCCCCAACAAAGAATCTTAAAATGAAGATTAGCCATCCAAGATTTGTAAGTCCTGGAAATCATGAAGAAGAGCTGACAACCGAATTTCCAGATTGCTTAAAGAAGGGCGATGAACATAATATTGTTCAGCGTGAAAGTAATAGAACAAGTAGTGTATTTGCATTTCCAAAGGATGTATCACTGCAAGGAGACAGATCATTAAGGTGGCCTCTTTGGCTTCGAGCTGCCATCCCAGGAACAATATCTTTGTATTTTACGATATACTATGAAATGGAAAATGTATCAAGCATGAAATACCGCACCCTAAGGATGCATTATAATTTGCAGGTTTTACCGTCTCTACAGACCTCGTTCAAAGTCACCCCATCTCCATCAAGATTGCAAGAATTTCTTGTGCGTATGGATATCGTGAACCGTGCGAAGTCAGATTGTTTCCAAATTCACCAGTTGTCAACAGTTGGGTGCCGGTGGGGTATCTCATTGCTTCAGCCTGTTGATACAATCTTACCTTCCAAATTTCTACTCGCTGGGCAAGCTTTATCTTGTTTCTTCATGATAAAGGATTGCAGAAAATCCGGGACTGAAGACGAGGAAACCACGTCCCTTCCTCCCAGCCAAACTGATGTAAAATTGTATACTCAGGATGACGATGAAAAGCTTTTCGATATCGTTAGCTCACCTTTAGCAAGCTTCCATGAAAGTGAAAGGTCATGTCAGGGACCCTCGGTTCAGGTAAGTCCAAATACTGTTGACTTCATCCTAATCTCTCGCCTGGCGAAAAGCAGCAGTCCATCAGCAGTTCCCGATTTACCAAAGATTTTGTCTCACCATTCATGTCATAACAGTATCAGAAGCTCGAGCCCGATCTCGTGGTCACTAGACGGTCCCCAAACCATACACCACGACTTTTCGACCTCCTTGTGTGAAATAAAACTAAAGATGGTAATCAGAAACACATCTGATGGAATATCATCTGTGACCATCAACACGAATGATTTTCTACCAGACGCAGCAGCAACTACTTCTTCCTCTGGAAACCAATCCGGGTGGCGCTACGTGCCAACCATCACAGAGGAAATGAAACTAACTTCAGATGTGATGGGAAGCCGTCTGGGAAAACCGCCATCTTCCATGGAAAGCTCACCTCCTTTCATATGGTCAGGTTTAAGTTCCACAAAGATCCAAATCCAGCCATTATCCACAACCGAAATACCGCTGCAGATATCAGTTTTCTCTCCAGGTATCTACGATCTGTCTTCATATGAACTTAACTGGGAGCTATCTGAGCATGAAAGTGCAACATCATCAGGGACATGCCAAGGTTATCCTTATTACCTTACTGTTCTTCAGTCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPPC-Trs85 TRAPPC9-Trs120

Protein Analysis

1272

Amino Acids

143.58

Weight (kDa)

6.2

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC-Trs85 PF12739 15 - 427 2.8e-85 ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Ig_TPPC8_1st PF24545 601 - 754 1.7e-13 TPPC8 first Ig-like domain
Ig_TPPC8_2nd PF24544 805 - 921 2.9e-09 TPPC8 second Ig-like domain
Ig_TPPC8_C PF24542 1108 - 1240 1.3e-10 TPPC8 C-terminal Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16280 AT5G16280
fragaria_vesca FvH4_6g20450 FvH4_6g20450 FvH4_6g20450 FvH4_6g20450
malus_domestica MD12G1041600.v1.1 MD14G1040700.v1.1
prunus_persica Prupe.7G093600_v2.0.a1
pyrus_communis pycom12g03630 pycom14g03450
rosa_chinensis RchiOBHm_Chr3g0476301
rosa_laevigata RLG00000023780
rosa_multiflora Rmu_sc0000114.1_g000012
rosa_roxburghii Rroxscaffold_6G00405410
rosa_rugosa Rorug03G0153400
rosa_samantha Rh3AG204600 Rh3BG236000 Rh3CG230700 Rh3DG230500
rosa_wichuraiana Rw3G018590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1503, 2778
Acc36I ACCTGC 1 cut(s) 2776
AccB1I GGYRCC 1 cut(s) 2922
AccI GTMKAC 3 cut(s) 977, 2365, 3095
AccIII TCCGGA 1 cut(s) 952
AciI CCGC 5 cut(s) 1315, 2016, 2758, 3581, 3674
AclWI GGATC 2 cut(s) 1953, 3634
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 9 cut(s) 506, 1008, 1846, 2020, 2345, 2543, 2846, 2898, 2975
AcuI CTGAAG 5 cut(s) 2157, 2930, 3063, 3534, 3791
AfaI GTAC 4 cut(s) 76, 418, 550, 1477
AfiI CCNNNNNNNGG 2 cut(s) 545, 1633
AflIII ACRYGT 1 cut(s) 280
AjiI CACGTC 1 cut(s) 3060
AjnI CCWGG 6 cut(s) 725, 2369, 2512, 2692, 3231, 3697
AjuI GAANNNNNNNTTGG 6 cut(s) 23, 55, 1156, 1188, 1635, 1667
AloI GAACNNNNNNTCC 2 cut(s) 3171, 3203
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 7 cut(s) 1428, 1743, 2001, 2173, 2643, 2802, 3288
AlwI GGATC 2 cut(s) 1953, 3634
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 3316
Aor13HI TCCGGA 1 cut(s) 952
AoxI GGCC 2 cut(s) 753, 2666
ApeKI GCWGC 7 cut(s) 1298, 1343, 2684, 3243, 3470, 3473, 3676
ApoI RAATTY 9 cut(s) 506, 1008, 1846, 2020, 2345, 2543, 2846, 2898, 2975
ArsI GACNNNNNNTTYG 2 cut(s) 3266, 3298
AspLEI GCGC 1 cut(s) 3513
AspS9I GGNCC 3 cut(s) 556, 3179, 3344
AsuC2I CCSGG 2 cut(s) 3037, 3505
AvaI CYCGRG 1 cut(s) 3316
AvaII GGWCC 3 cut(s) 556, 3179, 3344
AxyI CCTNAGG 2 cut(s) 101, 2765
BanI GGYRCC 1 cut(s) 2922
BanII GRGCYC 1 cut(s) 3323
BauI CACGAG 1 cut(s) 3328
BbsI GAAGAC 3 cut(s) 1764, 3051, 3708
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 7 cut(s) 1285, 1330, 2671, 3255, 3482, 3485, 3663
BceAI ACGGC 2 cut(s) 287, 3552
BcgI CGANNNNNNTGC 2 cut(s) 842, 876
BciT130I CCWGG 6 cut(s) 727, 2371, 2514, 2694, 3233, 3699
BciVI GTATCC 1 cut(s) 2219
BclI TGATCA 1 cut(s) 2389
BcnI CCSGG 2 cut(s) 3037, 3505
BcoDI GTCTC 7 cut(s) 1428, 1743, 2001, 2173, 2643, 2802, 3288
BfaI CTAG 4 cut(s) 275, 1278, 1421, 3338
BfmI CTRYAG 3 cut(s) 1344, 2801, 3677
BfoI RGCGCY 1 cut(s) 3514
BfuAI ACCTGC 1 cut(s) 2776
BfuI GTATCC 1 cut(s) 2219
BglI GCCNNNNNGGC 1 cut(s) 2674
BglII AGATCT 2 cut(s) 514, 805
BisI GCNGC 7 cut(s) 1299, 1344, 2685, 3244, 3471, 3474, 3677
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 7 cut(s) 1300, 1345, 2686, 3245, 3472, 3475, 3678
Bme1390I CCNGG 8 cut(s) 727, 2371, 2514, 2694, 3037, 3233, 3505, 3699
Bme18I GGWCC 3 cut(s) 556, 3179, 3344
BmeT110I CYCGRG 1 cut(s) 3316
BmgBI CACGTC 1 cut(s) 3060
BmgT120I GGNCC 3 cut(s) 556, 3179, 3344
BmiI GGNNCC 9 cut(s) 9, 558, 1332, 1520, 2248, 2924, 3180, 3181, 3346
BmrFI CCNGG 8 cut(s) 727, 2371, 2514, 2694, 3037, 3233, 3505, 3699
BmrI ACTGGG 2 cut(s) 2190, 3742
BmsI GCATC 9 cut(s) 592, 801, 946, 1257, 1566, 1784, 1815, 2326, 2760
BmuI ACTGGG 2 cut(s) 2190, 3742
BpiI GAAGAC 3 cut(s) 1764, 3051, 3708
BpmI CTGGAG 5 cut(s) 1841, 1947, 1970, 2436, 3681
Bpu1102I GCTNAGC 1 cut(s) 264
BpuEI CTTGAG 6 cut(s) 116, 276, 1764, 2047, 2089, 2396
BpuMI CCSGG 2 cut(s) 3037, 3505
Bsa29I ATCGAT 2 cut(s) 159, 1092
BsaAI YACGTR 2 cut(s) 416, 3517
BsaBI GATNNNNATC 2 cut(s) 3404, 3644
BsaI GGTCTC 1 cut(s) 1428
BsaJI CCNNGG 8 cut(s) 725, 726, 1632, 2369, 2692, 3183, 3591, 3778
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 4 cut(s) 1920, 1950, 2812, 2842
Bsc4I CCNNNNNNNGG 2 cut(s) 545, 1633
Bse118I RCCGGY 2 cut(s) 10, 2925
Bse1I ACTGG 8 cut(s) 56, 784, 1690, 1824, 1868, 2196, 2905, 3737
Bse21I CCTNAGG 2 cut(s) 101, 2765
Bse3DI GCAATG 2 cut(s) 1964, 2939
Bse8I GATNNNNATC 2 cut(s) 3404, 3644
BseAI TCCGGA 1 cut(s) 952
BseBI CCWGG 6 cut(s) 727, 2371, 2514, 2694, 3233, 3699
BseCI ATCGAT 2 cut(s) 159, 1092
BseDI CCNNGG 8 cut(s) 725, 726, 1632, 2369, 2692, 3183, 3591, 3778
BseJI GATNNNNATC 2 cut(s) 3404, 3644
BseLI CCNNNNNNNGG 2 cut(s) 545, 1633
BseMI GCAATG 2 cut(s) 1964, 2939
BseNI ACTGG 8 cut(s) 56, 784, 1690, 1824, 1868, 2196, 2905, 3737
BseRI GAGGAG 1 cut(s) 324
BseX3I CGGCCG 1 cut(s) 753
BseXI GCAGC 7 cut(s) 1285, 1330, 2671, 3255, 3482, 3485, 3663
BseYI CCCAGC 3 cut(s) 296, 2987, 3071
Bsh1285I CGRYCG 2 cut(s) 756, 993
BshFI GGCC 2 cut(s) 755, 2668
BshNI GGYRCC 1 cut(s) 2922
BshVI ATCGAT 2 cut(s) 159, 1092
BsiEI CGRYCG 2 cut(s) 756, 993
BsiHKAI GWGCWC 1 cut(s) 775
BsiHKCI CYCGRG 1 cut(s) 3316
BsiSI CCGG 5 cut(s) 11, 953, 2926, 3036, 3504
BslFI GGGAC 8 cut(s) 1149, 1673, 1722, 3046, 3052, 3192, 3330, 3784
BslI CCNNNNNNNGG 2 cut(s) 545, 1633
BsmAI GTCTC 7 cut(s) 1428, 1743, 2001, 2173, 2643, 2802, 3288
BsmBI CGTCTC 2 cut(s) 1743, 2802
BsmFI GGGAC 8 cut(s) 1149, 1673, 1722, 3046, 3052, 3192, 3330, 3784
BsmI GAATGC 2 cut(s) 671, 1193
BsnI GGCC 2 cut(s) 755, 2668
Bso31I GGTCTC 1 cut(s) 1428
BsoBI CYCGRG 1 cut(s) 3316
Bsp1286I GDGCHC 2 cut(s) 775, 3323
Bsp13I TCCGGA 1 cut(s) 952
Bsp1720I GCTNAGC 1 cut(s) 264
Bsp19I CCATGG 1 cut(s) 3591
BspACI CCGC 5 cut(s) 1315, 2016, 2758, 3581, 3674
BspANI GGCC 2 cut(s) 755, 2668
BspDI ATCGAT 2 cut(s) 159, 1092
BspEI TCCGGA 1 cut(s) 952
BspHI TCATGA 2 cut(s) 2521, 3012
BspLI GGNNCC 9 cut(s) 9, 558, 1332, 1520, 2248, 2924, 3180, 3181, 3346
BspMAI CTGCAG 2 cut(s) 1348, 3681
BspMI ACCTGC 1 cut(s) 2776
BspPI GGATC 2 cut(s) 1953, 3634
BspQI GCTCTTC 1 cut(s) 2523
BspT107I GGYRCC 1 cut(s) 2922
BspTNI GGTCTC 1 cut(s) 1428
BsrDI GCAATG 2 cut(s) 1964, 2939
BsrFI RCCGGY 2 cut(s) 10, 2925
BsrI ACTGG 8 cut(s) 56, 784, 1690, 1824, 1868, 2196, 2905, 3737
BssAI RCCGGY 2 cut(s) 10, 2925
BssECI CCNNGG 8 cut(s) 725, 726, 1632, 2369, 2692, 3183, 3591, 3778
BssNAI GTATAC 2 cut(s) 978, 3096
BssSI CACGAG 1 cut(s) 3328
BssT1I CCWWGG 3 cut(s) 1632, 3591, 3778
Bst1107I GTATAC 2 cut(s) 978, 3096
Bst2BI CACGAG 1 cut(s) 3328
Bst2UI CCWGG 6 cut(s) 727, 2371, 2514, 2694, 3233, 3699
Bst6I CTCTTC 4 cut(s) 25, 1746, 1794, 2523
BstAPI GCANNNNNTGC 1 cut(s) 2297
BstBAI YACGTR 2 cut(s) 416, 3517
BstC8I GCNNGC 5 cut(s) 1341, 1362, 1392, 2994, 3148
BstDSI CCRYGG 1 cut(s) 3591
BstH2I RGCGCY 1 cut(s) 3514
BstHHI GCGC 1 cut(s) 3513
BstMAI GTCTC 7 cut(s) 1428, 1743, 2001, 2173, 2643, 2802, 3288
BstMCI CGRYCG 2 cut(s) 756, 993
BstMWI GCNNNNNNNGC 7 cut(s) 196, 1781, 2297, 2674, 2993, 3517, 3745
BstNI CCWGG 6 cut(s) 727, 2371, 2514, 2694, 3233, 3699
BstNSI RCATGY 3 cut(s) 465, 1603, 3777
BstSCI CCNGG 8 cut(s) 725, 2369, 2512, 2692, 3035, 3231, 3503, 3697
BstSFI CTRYAG 3 cut(s) 1344, 2801, 3677
BstSNI TACGTA 1 cut(s) 416
BstV1I GCAGC 7 cut(s) 1285, 1330, 2671, 3255, 3482, 3485, 3663
BstV2I GAAGAC 3 cut(s) 1764, 3051, 3708
BstX2I RGATCY 4 cut(s) 514, 805, 1945, 3639
BstYI RGATCY 4 cut(s) 514, 805, 1945, 3639
BstZ17I GTATAC 2 cut(s) 978, 3096
BstZI CGGCCG 1 cut(s) 753
Bsu15I ATCGAT 2 cut(s) 159, 1092
Bsu36I CCTNAGG 2 cut(s) 101, 2765
BsuI GTATCC 1 cut(s) 2219
BsuRI GGCC 2 cut(s) 755, 2668
BsuTUI ATCGAT 2 cut(s) 159, 1092
BtgI CCRYGG 1 cut(s) 3591
BtgZI GCGATG 3 cut(s) 1409, 1748, 2583
BtrI CACGTC 1 cut(s) 3060
BtsI GCAGTG 1 cut(s) 2639
BtsIMutI CAGTG 8 cut(s) 63, 777, 1562, 1817, 2184, 2203, 2331, 2639
BveI ACCTGC 1 cut(s) 2776
Cac8I GCNNGC 5 cut(s) 1341, 1362, 1392, 2994, 3148
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 2 cut(s) 2521, 3012
CfoI GCGC 1 cut(s) 3513
Cfr10I RCCGGY 2 cut(s) 10, 2925
Cfr13I GGNCC 3 cut(s) 556, 3179, 3344
ClaI ATCGAT 2 cut(s) 159, 1092
CseI GACGC 1 cut(s) 3476
Csp6I GTAC 4 cut(s) 75, 417, 549, 1476
CspCI CAANNNNNGTGG 2 cut(s) 3488, 3523
CviQI GTAC 4 cut(s) 75, 417, 549, 1476
DraI TTTAAA 2 cut(s) 739, 916
EaeI YGGCCR 1 cut(s) 753
EagI CGGCCG 1 cut(s) 753
Eam1104I CTCTTC 4 cut(s) 25, 1746, 1794, 2523
EarI CTCTTC 4 cut(s) 25, 1746, 1794, 2523
EclXI CGGCCG 1 cut(s) 753
Eco105I TACGTA 1 cut(s) 416
Eco130I CCWWGG 3 cut(s) 1632, 3591, 3778
Eco24I GRGCYC 1 cut(s) 3323
Eco31I GGTCTC 1 cut(s) 1428
Eco32I GATATC 4 cut(s) 1831, 2866, 3127, 3684
Eco47I GGWCC 3 cut(s) 556, 3179, 3344
Eco52I CGGCCG 1 cut(s) 753
Eco57I CTGAAG 5 cut(s) 2157, 2930, 3063, 3534, 3791
Eco81I CCTNAGG 2 cut(s) 101, 2765
Eco88I CYCGRG 1 cut(s) 3316
EcoO109I RGGNCCY 1 cut(s) 3179
EcoRII CCWGG 6 cut(s) 725, 2369, 2512, 2692, 3231, 3697
EcoRV GATATC 4 cut(s) 1831, 2866, 3127, 3684
EcoT14I CCWWGG 3 cut(s) 1632, 3591, 3778
EcoT22I ATGCAT 4 cut(s) 1195, 1581, 1777, 2775
EcoT38I GRGCYC 1 cut(s) 3323
ErhI CCWWGG 3 cut(s) 1632, 3591, 3778
Esp3I CGTCTC 2 cut(s) 1743, 2802
FalI AAGNNNNNCTT 6 cut(s) 563, 595, 1211, 1243, 2835, 2867
FaqI GGGAC 8 cut(s) 1149, 1673, 1722, 3046, 3052, 3192, 3330, 3784
FauNDI CATATG 3 cut(s) 1319, 3614, 3721
FbaI TGATCA 1 cut(s) 2389
FblI GTMKAC 3 cut(s) 977, 2365, 3095
Fnu4HI GCNGC 7 cut(s) 1299, 1344, 2685, 3244, 3471, 3474, 3677
FriOI GRGCYC 1 cut(s) 3323
Fsp4HI GCNGC 7 cut(s) 1299, 1344, 2685, 3244, 3471, 3474, 3677
FspBI CTAG 4 cut(s) 275, 1278, 1421, 3338
GlaI GCGC 1 cut(s) 3512
GluI GCNGC 7 cut(s) 1299, 1344, 2685, 3244, 3471, 3474, 3677
GsaI CCCAGC 3 cut(s) 300, 2991, 3075
GsuI CTGGAG 5 cut(s) 1841, 1947, 1970, 2436, 3681
HaeII RGCGCY 1 cut(s) 3514
HaeIII GGCC 2 cut(s) 755, 2668
HapII CCGG 5 cut(s) 11, 953, 2926, 3036, 3504
HgaI GACGC 1 cut(s) 3476
HhaI GCGC 1 cut(s) 3513
Hin6I GCGC 1 cut(s) 3511
HinP1I GCGC 1 cut(s) 3511
HincII GTYRAC 4 cut(s) 1383, 1462, 2913, 3211
HindII GTYRAC 4 cut(s) 1383, 1462, 2913, 3211
HindIII AAGCTT 9 cut(s) 441, 1098, 1362, 1782, 1883, 2065, 2994, 3116, 3148
HpaI GTTAAC 2 cut(s) 1383, 1462
HpaII CCGG 5 cut(s) 11, 953, 2926, 3036, 3504
HpyAV CCTTC 7 cut(s) 298, 1036, 2198, 2304, 2557, 2979, 3074
HpyCH4IV ACGT 8 cut(s) 252, 280, 415, 620, 705, 2425, 3059, 3516
HpyF10VI GCNNNNNNNGC 7 cut(s) 196, 1781, 2297, 2674, 2993, 3517, 3745
HpySE526I ACGT 8 cut(s) 252, 280, 415, 620, 705, 2425, 3059, 3516
HspAI GCGC 1 cut(s) 3511
KflI GGGWCCC 1 cut(s) 3179
Kpn2I TCCGGA 1 cut(s) 952
Ksp22I TGATCA 1 cut(s) 2389
KspAI GTTAAC 2 cut(s) 1383, 1462
LguI GCTCTTC 1 cut(s) 2523
LmnI GCTCC 5 cut(s) 7, 850, 1357, 1518, 3736
Lsp1109I GCAGC 7 cut(s) 1285, 1330, 2671, 3255, 3482, 3485, 3663
LweI GCATC 9 cut(s) 592, 801, 946, 1257, 1566, 1784, 1815, 2326, 2760
MaeI CTAG 4 cut(s) 275, 1278, 1421, 3338
MaeII ACGT 8 cut(s) 252, 280, 415, 620, 705, 2425, 3059, 3516
MaeIII GTNAC 8 cut(s) 152, 1016, 1398, 2101, 2188, 2818, 3333, 3436
MfeI CAATTG 2 cut(s) 1566, 2385
MflI RGATCY 4 cut(s) 514, 805, 1945, 3639
MhlI GDGCHC 2 cut(s) 775, 3323
MlyI GAGTC 5 cut(s) 374, 1940, 2080, 2175, 2371
MmeI TCCRAC 1 cut(s) 1335
Mph1103I ATGCAT 4 cut(s) 1195, 1581, 1777, 2775
MroI TCCGGA 1 cut(s) 952
MslI CAYNNNNRTG 8 cut(s) 161, 382, 1041, 1205, 1407, 2662, 3159, 3753
MspA1I CMGCKG 2 cut(s) 190, 3676
MspI CCGG 5 cut(s) 11, 953, 2926, 3036, 3504
MspR9I CCNGG 8 cut(s) 727, 2371, 2514, 2694, 3037, 3233, 3505, 3699
MunI CAATTG 2 cut(s) 1566, 2385
Mva1269I GAATGC 2 cut(s) 671, 1193
MvaI CCWGG 6 cut(s) 727, 2371, 2514, 2694, 3233, 3699
MwoI GCNNNNNNNGC 7 cut(s) 196, 1781, 2297, 2674, 2993, 3517, 3745
NciI CCSGG 2 cut(s) 3037, 3505
NcoI CCATGG 1 cut(s) 3591
NdeI CATATG 3 cut(s) 1319, 3614, 3721
NlaIV GGNNCC 9 cut(s) 9, 558, 1332, 1520, 2248, 2924, 3180, 3181, 3346
NmuCI GTSAC 4 cut(s) 1016, 2818, 3333, 3436
NsiI ATGCAT 4 cut(s) 1195, 1581, 1777, 2775
NspI RCATGY 3 cut(s) 465, 1603, 3777
PaeR7I CTCGAG 1 cut(s) 3316
PagI TCATGA 2 cut(s) 2521, 3012
PasI CCCWGGG 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 2523
PcsI WCGNNNNNNNCGW 2 cut(s) 277, 444
PctI GAATGC 2 cut(s) 671, 1193
PfeI GAWTC 6 cut(s) 995, 1172, 1907, 1960, 2459, 2473
PfoI TCCNGGA 2 cut(s) 2512, 3035
PkrI GCNGC 7 cut(s) 1300, 1345, 2686, 3245, 3472, 3475, 3678
PleI GAGTC 5 cut(s) 373, 1939, 2079, 2175, 2370
PpsI GAGTC 5 cut(s) 373, 1939, 2079, 2175, 2370
Ppu21I YACGTR 2 cut(s) 416, 3517
PpuMI RGGWCCY 1 cut(s) 3179
PsiI TTATAA 2 cut(s) 1503, 2778
Psp5II RGGWCCY 1 cut(s) 3179
Psp6I CCWGG 6 cut(s) 725, 2369, 2512, 2692, 3231, 3697
PspFI CCCAGC 3 cut(s) 296, 2987, 3071
PspGI CCWGG 6 cut(s) 725, 2369, 2512, 2692, 3231, 3697
PspN4I GGNNCC 9 cut(s) 9, 558, 1332, 1520, 2248, 2924, 3180, 3181, 3346
PspPI GGNCC 3 cut(s) 556, 3179, 3344
PspPPI RGGWCCY 1 cut(s) 3179
PspXI VCTCGAGB 1 cut(s) 3316
PstI CTGCAG 2 cut(s) 1348, 3681
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 4 cut(s) 514, 805, 1945, 3639
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 4 cut(s) 76, 418, 550, 1477
RsaNI GTAC 4 cut(s) 75, 417, 549, 1476
RseI CAYNNNNRTG 8 cut(s) 161, 382, 1041, 1205, 1407, 2662, 3159, 3753
SapI GCTCTTC 1 cut(s) 2523
SatI GCNGC 7 cut(s) 1299, 1344, 2685, 3244, 3471, 3474, 3677
Sau96I GGNCC 3 cut(s) 556, 3179, 3344
SchI GAGTC 5 cut(s) 374, 1940, 2080, 2175, 2371
ScrFI CCNGG 8 cut(s) 727, 2371, 2514, 2694, 3037, 3233, 3505, 3699
SduI GDGCHC 2 cut(s) 775, 3323
SfaNI GCATC 9 cut(s) 592, 801, 946, 1257, 1566, 1784, 1815, 2326, 2760
SfcI CTRYAG 3 cut(s) 1344, 2801, 3677
Sfr274I CTCGAG 1 cut(s) 3316
SinI GGWCC 3 cut(s) 556, 3179, 3344
SlaI CTCGAG 1 cut(s) 3316
SmiI ATTTAAAT 1 cut(s) 739
SmiMI CAYNNNNRTG 8 cut(s) 161, 382, 1041, 1205, 1407, 2662, 3159, 3753
SmlI CTYRAG 7 cut(s) 131, 291, 1779, 2062, 2068, 2375, 3316
SmoI CTYRAG 7 cut(s) 131, 291, 1779, 2062, 2068, 2375, 3316
SnaBI TACGTA 1 cut(s) 416
SsiI CCGC 5 cut(s) 1315, 2016, 2758, 3581, 3674
SspI AATATT 1 cut(s) 2581
SspMI CTAG 4 cut(s) 275, 1278, 1421, 3338
StyD4I CCNGG 8 cut(s) 725, 2369, 2512, 2692, 3035, 3231, 3503, 3697
StyI CCWWGG 3 cut(s) 1632, 3591, 3778
SwaI ATTTAAAT 1 cut(s) 739
TaiI ACGT 8 cut(s) 255, 283, 418, 623, 708, 2428, 3062, 3519
TaqII GACCGA 1 cut(s) 1007
TfiI GAWTC 6 cut(s) 995, 1172, 1907, 1960, 2459, 2473
TscAI CASTG 8 cut(s) 63, 784, 1562, 1824, 2191, 2203, 2338, 2646
TseFI GTSAC 4 cut(s) 1016, 2818, 3333, 3436
TseI GCWGC 7 cut(s) 1298, 1343, 2684, 3243, 3470, 3473, 3676
Tsp45I GTSAC 4 cut(s) 1016, 2818, 3333, 3436
TspRI CASTG 8 cut(s) 63, 784, 1562, 1824, 2191, 2203, 2338, 2646
VpaK11BI GGWCC 3 cut(s) 556, 3179, 3344
XapI RAATTY 9 cut(s) 506, 1008, 1846, 2020, 2345, 2543, 2846, 2898, 2975
XceI RCATGY 3 cut(s) 465, 1603, 3777
XhoI CTCGAG 1 cut(s) 3316
XmiI GTMKAC 3 cut(s) 977, 2365, 3095
XspI CTAG 4 cut(s) 275, 1278, 1421, 3338
Zsp2I ATGCAT 4 cut(s) 1195, 1581, 1777, 2775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.