Rh3DG230500

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0010066
Physical Location & Seq
Forward (+)
21894589 .. 21903614
9026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG230500.1

Sequence Viewer

Length: 3033 bp
ATGCAAGATTTTTCATCTAAGCACATCATCCCTTACATGGAGCAAAAAATACGTGTGCTGAATCAGCAGGTGTCTGCAACAAGAAAGGGTTTCCGGAACCAAATAAAAAATTTGTGGTGGAGAAAAGGGAAAGACGAGGTGGTGGATTCACCCAGCGGCCCTACGTATACCTTTAGCTCCATTGAATCTCAGATTAGAGTTCTGGGTGATTACGCCTTCATGCTACGTGATTATGAACTTGCATTATCGAACTATCGCCTAATATCAACAGATTATAAGCTTGATAAAGCCTGGAAACGCTTTGCTGGTGCACAGGAAATGATGGGGCTTGCATATTTCATGTTGGACCACTCAAGAAAGGAGGCAGAGAGTTGCATGGATACTGCATTTACTTACTATCTAAAATATGCATTATCTAGTCAGCAGAACGCAACACGATGTGGTCTTTGGTGGGTAGAGATGTTGAAGGCAAGATATCAGTATAGAGAGGCTGCAACTGTTTACTTTCGTGTTGGTGCTGAGGAGCCTCTACATTCTGCTGTAATGCTTGAGCAAGCATCCTATTGCTACTTATTGTCCAAACCACCTATGTTACACAAATATGGGTTTCATCTTGTTCTTTCTGGTGATCGTTACAAGAAATGTGATCAGATCAAACATGCAATTCGCACATATAGAAGTGCCATGTCTGTATATAAAGGAACTACATGGGGCCATATTACAGACCATATCCATTTCCACCTTGGACAGTGGTATGCTCTTCTGGGGTTATATGATTTGGCTGTCTCACATATGTTGGAGGTTCTGGCGTGTAGGCATCAGTCCAAGACAATGCAGGAGTTATTCCTGAAGGACTTCCTTGAAATTGTTCAGAAAACAGGAAAAACATTTGAGGTTTCAAAGCTTCAGTTGCCTGAGATCAATATTCCATCTCTTCGGGTCTTCTTTGAAGATCATCGAACCTATGCATCATCTGCAGCTGCTTCTGTTAAAGAAAGATTATGGCGTTCTTTAGAGGAGGAAATGGTTCCATCTACGTCAACTGGCAGAACTAATTGGCTGGAGTTACAATCAAAGCTTATCCCAAAGAAGTTTAAAGAATCAAATATATGTGTTGCAGGAGAAGCAGTCAAGATTGATATTGAGTTTAAGAACCCTCTGCAAATTCCTCTCCTCCTATCCAGTGTTTCCTTAATATGTGAGCTTTCTACCAACTCTGATGAAATGAAATCCGATGAAAATAATTCATTGGTGGGCGTCCAAAATAATGATGAGTCTACCAAATTAAACCACAGGGATGTGGACTCTGAGACATTGTTTTCTTTGTCTGATGTGGACGTTTCAATGGGAGGAGGTGAAACAACTCTGGTGCAACTAACAGTTACTCCCAGAGTAGAAGGTGTTTTACAAATTGTTGGCGTAAAGTGGAAATTATCAGATTTTGTTGTTGGTTTCCATAACTTTGACACTAGACCTGTGAAGATAATCGGAAAGCAGAGACAGAAGGCCAATCATCCTCTAAGTATTAATTTAAAGTTTGCAGTGGTTAAGAGTGTACCCAAGCTTGAAGGTGTCATTCATTCACTACCTGAAAGGGCGTATGCAGGGGATGTACGGCACCTTGTGTTGGAGTTGAAGAACCAATCAGAATTTGCTGTCAAGAATCTGAAAATGAAGATTAGTCATCCTAGATTCCTAAATGTTGGGCAACGGGAAAGTTTGAACTCAGAGTTTCCTGCTTGCTTAGAAAAGAAAACATCTCAGCATTCTGATATACGTTCCGACTCTGACGTGTCTCATTCTGTGTTTCTATTTCCAGAGGATACTACAATTCAAGGGGAAAAACCATTGCTGTGGCCTCTTTGGTTTCGGGCAGCACTACCTGGGAACATCTCGTTGGACATATCTATATATTATGAGATGGGAGATGTGTCAAGCGGAATTAAATATCGCACTTTACGCATGCATTACAATCTACAGGTATTGCCATCGTTGGATGTGTCATTCCAAATCAGTCCTTGTCCATCAAGATTGCGTGAGTTCCTTGTGCGCATGGATGTTGTCAACAAGACCAGCTCAGAGAGTTTCCAAGTTCATCAGTTATCATCTGTTGGTCAGAAATGGGAGGTCTCATTACTTCAACCTGTCGATGCCATCTTTCCTTCACAATCTTTAATGGCTCATCAAGCATTATCATGCTTCTTCATGCTTAAGAGTCGTAGCAAGCCATCAACTTCTGAAGATGAGACATCCTCTTGTTCCCCGCTTCTGGGAAGTGATGTGACATTGGGTACTGAGGGCAGCTGTGGACCACTTTTCGATATAGCTAGCTCGCCACTGGCGGATTTCCACCATTGTGAAAGACTGCAGCAGGAAATTTCACATAAGGGAGACGTGAATACAGTTGACTTCATATTGATCTCTCGGCCTCTGAAGAATGATACTCACCCTGTGGTTTCTGATCTACCACATCTTTTTTCTCATCATGCATGTCACTGCAGTATCGAGAGCAGAAGTCCCATATCGTGGCTGGTGGATGGACCTCGAACCTTGTATCATAACTTCTCAGCTTCCTTTTGTGAAATAAATTTTCACATAACTATATACAACTCATCAGATGTCATTGCATCTGTATGCATTAAAACCTACGACTCCAACAGTGATAATTTAAGTGATGCAATTCCAGTTCAACCTGCCATGTCTTCTAGCAACCAAGATGGATGGCATGATCTCCCACTAGTTAATGAAATTAGAGTGACTTCTGATGTCGTTGGAGCTCGGACTGGGAAGTCATCGTCAGTGGAGAGTGTTACCCCATTTATTTGGTCTGGGTCAAGCTCTACTAAAGTCGAACTGGAGCCTAAGTCAAGAACTGAGATTCCCCTTCAAGTTTGTGTATTCTCTCCCGGTACATTCGATTTGTCAAATTATGTTTTGCATTGGAAACTTTTGCTTTCTAATGGTGATGGTCTACAGTCTTCTGGGGCATGCCAGGGATATCCTTATTATCTTACCGTGCTCCAGTCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPPC-Trs85 TRAPPC9-Trs120

Protein Analysis

1010

Amino Acids

114.11

Weight (kDa)

6.61

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC-Trs85 PF12739 1 - 158 1.4e-39 ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Ig_TPPC8_1st PF24545 332 - 484 2e-16 TPPC8 first Ig-like domain
Ig_TPPC8_2nd PF24544 536 - 653 6e-08 TPPC8 second Ig-like domain
Ig_TPPC8_C PF24542 918 - 976 1.5e-07 TPPC8 C-terminal Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16280 AT5G16280
fragaria_vesca FvH4_6g20450 FvH4_6g20450 FvH4_6g20450 FvH4_6g20450
malus_domestica MD12G1041600.v1.1 MD14G1040700.v1.1
prunus_persica Prupe.7G093600_v2.0.a1
pyrus_communis pycom12g03630 pycom14g03450
rosa_chinensis RchiOBHm_Chr3g0476301
rosa_laevigata RLG00000023780
rosa_multiflora Rmu_sc0000114.1_g000012
rosa_roxburghii Rroxscaffold_6G00405410
rosa_rugosa Rorug03G0153400
rosa_samantha Rh3AG204600 Rh3BG236000 Rh3CG230700 Rh3DG230500
rosa_wichuraiana Rw3G018590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AarI CACCTGC 1 cut(s) 58
AasI GACNNNNNNGTC 1 cut(s) 2788
Acc16I TGCGCA 1 cut(s) 2051
Acc36I ACCTGC 2 cut(s) 58, 2701
AccB1I GGYRCC 1 cut(s) 1617
AccB7I CCANNNNNTGG 1 cut(s) 2526
AccI GTMKAC 3 cut(s) 167, 1277, 2971
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 4 cut(s) 156, 1938, 2264, 2342
AcsI RAATTY 5 cut(s) 109, 1164, 1649, 2376, 2587
AcuI CTGAAG 4 cut(s) 869, 890, 2259, 2453
AcyI GRCGYC 1 cut(s) 1257
AdeI CACNNNGTG 3 cut(s) 440, 1624, 2452
AfaI GTAC 4 cut(s) 1557, 1614, 2293, 2911
AfiI CCNNNNNNNGG 5 cut(s) 37, 1627, 2269, 2270, 2526
AflII CTTAAG 1 cut(s) 2210
AflIII ACRYGT 2 cut(s) 52, 1791
AhlI ACTAGT 1 cut(s) 2737
AjiI CACGTC 2 cut(s) 1792, 2395
AjnI CCWGG 3 cut(s) 290, 1882, 2991
AjuI GAANNNNNNNTTGG 2 cut(s) 1880, 1912
AleI CACNNNNGTG 1 cut(s) 2355
AloI GAACNNNNNNTCC 2 cut(s) 2863, 2895
Alw21I GWGCWC 3 cut(s) 313, 2779, 3021
Alw26I GTCTC 7 cut(s) 790, 1304, 1492, 1800, 2134, 2240, 2385
Alw44I GTGCAC 1 cut(s) 309
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 5 cut(s) 157, 712, 1506, 1856, 2426
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 6 cut(s) 491, 977, 980, 1874, 2301, 2368
ApoI RAATTY 5 cut(s) 109, 1164, 1649, 2376, 2587
AseI ATTAAT 1 cut(s) 1527
Asp700I GAANNNNTTC 3 cut(s) 854, 867, 1026
AspLEI GCGC 1 cut(s) 2052
AspS9I GGNCC 5 cut(s) 158, 346, 712, 2309, 2540
AsuC2I CCSGG 1 cut(s) 2907
AsuHPI GGTGA 6 cut(s) 141, 218, 638, 1367, 2438, 2975
AsuNHI GCTAGC 1 cut(s) 2327
AvaII GGWCC 3 cut(s) 346, 2309, 2540
BaeGI GKGCMC 1 cut(s) 313
BaeI ACNNNNGTAYC 2 cut(s) 2485, 2518
BanI GGYRCC 1 cut(s) 1617
BanII GRGCYC 1 cut(s) 2779
BarI GAAGNNNNNNTAC 4 cut(s) 1389, 1421, 2546, 2578
BbsI GAAGAC 3 cut(s) 934, 2694, 2970
Bbv12I GWGCWC 3 cut(s) 313, 2779, 3021
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 6 cut(s) 478, 967, 989, 1886, 2313, 2380
BceAI ACGGC 1 cut(s) 1631
BciT130I CCWGG 3 cut(s) 292, 1884, 2993
BciVI GTATCC 2 cut(s) 373, 1816
BclI TGATCA 1 cut(s) 646
BcnI CCSGG 1 cut(s) 2907
BcoDI GTCTC 7 cut(s) 790, 1304, 1492, 1800, 2134, 2240, 2385
BcuI ACTAGT 1 cut(s) 2737
BfaI CTAG 6 cut(s) 417, 1470, 1689, 2328, 2706, 2738
BfmI CTRYAG 5 cut(s) 975, 1976, 2366, 2497, 2972
BfrI CTTAAG 1 cut(s) 2210
BfuAI ACCTGC 2 cut(s) 58, 2701
BfuI GTATCC 2 cut(s) 373, 1816
BisI GCNGC 7 cut(s) 157, 492, 978, 981, 1875, 2302, 2369
BlsI GCNGC 7 cut(s) 158, 493, 979, 982, 1876, 2303, 2370
Bme1390I CCNGG 4 cut(s) 292, 1884, 2907, 2993
Bme18I GGWCC 3 cut(s) 346, 2309, 2540
BmgBI CACGTC 2 cut(s) 1792, 2395
BmgT120I GGNCC 5 cut(s) 158, 346, 712, 2309, 2540
BmiI GGNNCC 6 cut(s) 98, 525, 713, 1029, 1619, 2859
BmrFI CCNGG 4 cut(s) 292, 1884, 2907, 2993
BmrI ACTGGG 1 cut(s) 2793
BmsI GCATC 6 cut(s) 566, 826, 977, 2140, 2636, 2665
BmtI GCTAGC 1 cut(s) 2331
BmuI ACTGGG 1 cut(s) 2793
BpiI GAAGAC 3 cut(s) 934, 2694, 2970
BplI GAGNNNNNCTC 2 cut(s) 2237, 2269
BpmI CTGGAG 3 cut(s) 1082, 2876, 3005
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 2 cut(s) 337, 569
BpuMI CCSGG 1 cut(s) 2907
BsaAI YACGTR 3 cut(s) 53, 165, 227
BsaHI GRCGYC 1 cut(s) 1257
BsaI GGTCTC 1 cut(s) 2134
BsaJI CCNNGG 3 cut(s) 742, 1883, 2992
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 6 cut(s) 1113, 1143, 1369, 1399, 2849, 2879
Bsc4I CCNNNNNNNGG 5 cut(s) 37, 1627, 2269, 2270, 2526
Bse1I ACTGG 7 cut(s) 1048, 1182, 2343, 2684, 2788, 2859, 3022
Bse3DI GCAATG 2 cut(s) 1847, 2622
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 3 cut(s) 292, 1884, 2993
BseDI CCNNGG 3 cut(s) 742, 1883, 2992
BseLI CCNNNNNNNGG 5 cut(s) 37, 1627, 2269, 2270, 2526
BseMI GCAATG 2 cut(s) 1847, 2622
BseNI ACTGG 7 cut(s) 1048, 1182, 2343, 2684, 2788, 2859, 3022
BseRI GAGGAG 4 cut(s) 536, 1031, 1163, 1365
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 6 cut(s) 478, 967, 989, 1886, 2313, 2380
BseYI CCCAGC 1 cut(s) 152
BshFI GGCC 5 cut(s) 159, 714, 1508, 1858, 2428
BshNI GGYRCC 1 cut(s) 1617
BsiHKAI GWGCWC 3 cut(s) 313, 2779, 3021
BsiSI CCGG 2 cut(s) 94, 2907
BslFI GGGAC 1 cut(s) 2502
BslI CCNNNNNNNGG 5 cut(s) 37, 1627, 2269, 2270, 2526
BsmAI GTCTC 7 cut(s) 790, 1304, 1492, 1800, 2134, 2240, 2385
BsmBI CGTCTC 1 cut(s) 2385
BsmFI GGGAC 1 cut(s) 2502
BsmI GAATGC 1 cut(s) 1765
BsnI GGCC 5 cut(s) 159, 714, 1508, 1858, 2428
Bso31I GGTCTC 1 cut(s) 2134
Bsp1286I GDGCHC 3 cut(s) 313, 2779, 3021
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
BspACI CCGC 4 cut(s) 156, 1938, 2264, 2342
BspANI GGCC 5 cut(s) 159, 714, 1508, 1858, 2428
BspEI TCCGGA 1 cut(s) 93
BspLI GGNNCC 6 cut(s) 98, 525, 713, 1029, 1619, 2859
BspMAI CTGCAG 3 cut(s) 979, 2370, 2501
BspMI ACCTGC 2 cut(s) 58, 2701
BspOI GCTAGC 1 cut(s) 2331
BspQI GCTCTTC 1 cut(s) 765
BspT107I GGYRCC 1 cut(s) 1617
BspTI CTTAAG 1 cut(s) 2210
BspTNI GGTCTC 1 cut(s) 2134
BsrDI GCAATG 2 cut(s) 1847, 2622
BsrI ACTGG 7 cut(s) 1048, 1182, 2343, 2684, 2788, 2859, 3022
BssECI CCNNGG 3 cut(s) 742, 1883, 2992
BssMI GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
BssNAI GTATAC 1 cut(s) 168
BssNI GRCGYC 1 cut(s) 1257
BssT1I CCWWGG 1 cut(s) 742
Bst1107I GTATAC 1 cut(s) 168
Bst2UI CCWGG 3 cut(s) 292, 1884, 2993
Bst4CI ACNGT 7 cut(s) 499, 750, 1381, 2404, 2660, 2976, 3016
Bst6I CTCTTC 2 cut(s) 765, 939
BstACI GRCGYC 1 cut(s) 1257
BstAFI CTTAAG 1 cut(s) 2210
BstAPI GCANNNNNTGC 1 cut(s) 974
BstBAI YACGTR 3 cut(s) 53, 165, 227
BstC8I GCNNGC 8 cut(s) 330, 555, 1741, 1964, 2225, 2329, 2333, 2989
BstHHI GCGC 1 cut(s) 2052
BstKTI GATC 8 cut(s) 631, 649, 654, 921, 955, 2421, 2464, 2731
BstMAI GTCTC 7 cut(s) 790, 1304, 1492, 1800, 2134, 2240, 2385
BstMBI GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
BstMWI GCNNNNNNNGC 6 cut(s) 64, 910, 974, 1124, 1959, 2186
BstNI CCWGG 3 cut(s) 292, 1884, 2993
BstNSI RCATGY 4 cut(s) 662, 1966, 2493, 2991
BstSCI CCNGG 4 cut(s) 290, 1882, 2905, 2991
BstSFI CTRYAG 5 cut(s) 975, 1976, 2366, 2497, 2972
BstSLI GKGCMC 1 cut(s) 313
BstSNI TACGTA 1 cut(s) 165
BstV1I GCAGC 6 cut(s) 478, 967, 989, 1886, 2313, 2380
BstV2I GAAGAC 3 cut(s) 934, 2694, 2970
BstXI CCANNNNNNTGG 2 cut(s) 1854, 2823
BstZ17I GTATAC 1 cut(s) 168
BsuI GTATCC 2 cut(s) 373, 1816
BsuRI GGCC 5 cut(s) 159, 714, 1508, 1858, 2428
BtrI CACGTC 2 cut(s) 1792, 2395
BtsI GCAGTG 2 cut(s) 1548, 2494
BtsIMutI CAGTG 7 cut(s) 755, 1189, 1548, 2336, 2494, 2665, 2805
BveI ACCTGC 2 cut(s) 58, 2701
Cac8I GCNNGC 8 cut(s) 330, 555, 1741, 1964, 2225, 2329, 2333, 2989
CfoI GCGC 1 cut(s) 2052
Cfr13I GGNCC 5 cut(s) 158, 346, 712, 2309, 2540
CseI GACGC 1 cut(s) 1246
Csp6I GTAC 4 cut(s) 1556, 1613, 2292, 2910
CviQI GTAC 4 cut(s) 1556, 1613, 2292, 2910
DpnI GATC 8 cut(s) 630, 648, 653, 920, 954, 2420, 2463, 2730
DpnII GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
DraI TTTAAA 2 cut(s) 1096, 1533
DraIII CACNNNGTG 3 cut(s) 440, 1624, 2452
DrdI GACNNNNNNGTC 1 cut(s) 2788
DseDI GACNNNNNNGTC 1 cut(s) 2788
Eam1104I CTCTTC 2 cut(s) 765, 939
EarI CTCTTC 2 cut(s) 765, 939
EciI GGCGGA 1 cut(s) 2357
Ecl136II GAGCTC 1 cut(s) 2777
Eco105I TACGTA 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 742
Eco24I GRGCYC 1 cut(s) 2779
Eco31I GGTCTC 1 cut(s) 2134
Eco32I GATATC 2 cut(s) 476, 2999
Eco47I GGWCC 3 cut(s) 346, 2309, 2540
Eco53kI GAGCTC 1 cut(s) 2777
Eco57I CTGAAG 4 cut(s) 869, 890, 2259, 2453
EcoICRI GAGCTC 1 cut(s) 2777
EcoRII CCWGG 3 cut(s) 290, 1882, 2991
EcoRV GATATC 2 cut(s) 476, 2999
EcoT14I CCWWGG 1 cut(s) 742
EcoT22I ATGCAT 5 cut(s) 412, 970, 1968, 2491, 2639
EcoT38I GRGCYC 1 cut(s) 2779
ErhI CCWWGG 1 cut(s) 742
Esp3I CGTCTC 1 cut(s) 2385
FaqI GGGAC 1 cut(s) 2502
FauI CCCGC 1 cut(s) 2271
FauNDI CATATG 1 cut(s) 792
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 3 cut(s) 167, 1277, 2971
Fnu4HI GCNGC 7 cut(s) 157, 492, 978, 981, 1875, 2302, 2369
FriOI GRGCYC 1 cut(s) 2779
Fsp4HI GCNGC 7 cut(s) 157, 492, 978, 981, 1875, 2302, 2369
FspAI RTGCGCAY 1 cut(s) 2051
FspBI CTAG 6 cut(s) 417, 1470, 1689, 2328, 2706, 2738
FspI TGCGCA 1 cut(s) 2051
GlaI GCGC 1 cut(s) 2051
GluI GCNGC 7 cut(s) 157, 492, 978, 981, 1875, 2302, 2369
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 3 cut(s) 1082, 2876, 3005
HaeIII GGCC 5 cut(s) 159, 714, 1508, 1858, 2428
HapII CCGG 2 cut(s) 94, 2907
HgaI GACGC 1 cut(s) 1246
HhaI GCGC 1 cut(s) 2052
Hin1I GRCGYC 1 cut(s) 1257
Hin6I GCGC 1 cut(s) 2050
HinP1I GCGC 1 cut(s) 2050
HincII GTYRAC 3 cut(s) 1041, 2065, 2407
HindII GTYRAC 3 cut(s) 1041, 2065, 2407
HindIII AAGCTT 4 cut(s) 278, 902, 1076, 1562
HpaII CCGG 2 cut(s) 94, 2907
HphI GGTGA 6 cut(s) 141, 218, 638, 1367, 2438, 2975
Hpy188III TCNNGA 9 cut(s) 94, 354, 847, 1132, 1660, 1817, 2028, 2506, 2868
HpyAV CCTTC 8 cut(s) 226, 460, 844, 1391, 1498, 1562, 2172, 2894
HpyCH4III ACNGT 7 cut(s) 499, 750, 1381, 2404, 2660, 2976, 3016
HpyCH4IV ACGT 8 cut(s) 52, 164, 226, 1037, 1338, 1777, 1791, 2394
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 910, 974, 1124, 1959, 2186
HpySE526I ACGT 8 cut(s) 52, 164, 226, 1037, 1338, 1777, 1791, 2394
Hsp92I GRCGYC 1 cut(s) 1257
HspAI GCGC 1 cut(s) 2050
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
LguI GCTCTTC 1 cut(s) 765
LmnI GCTCC 6 cut(s) 40, 182, 523, 2774, 2857, 3024
Lsp1109I GCAGC 6 cut(s) 478, 967, 989, 1886, 2313, 2380
LweI GCATC 6 cut(s) 566, 826, 977, 2140, 2636, 2665
MaeI CTAG 6 cut(s) 417, 1470, 1689, 2328, 2706, 2738
MaeII ACGT 8 cut(s) 52, 164, 226, 1037, 1338, 1777, 1791, 2394
MaeIII GTNAC 8 cut(s) 591, 632, 1065, 1381, 2281, 2492, 2755, 2809
MalI GATC 8 cut(s) 630, 648, 653, 920, 954, 2420, 2463, 2730
MboI GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
MhlI GDGCHC 3 cut(s) 313, 2779, 3021
MlyI GAGTC 5 cut(s) 1283, 1298, 1778, 2224, 2645
MmeI TCCRAC 8 cut(s) 324, 777, 1608, 1806, 1878, 1974, 2679, 2752
Mph1103I ATGCAT 5 cut(s) 412, 970, 1968, 2491, 2639
MroI TCCGGA 1 cut(s) 93
MroXI GAANNNNTTC 3 cut(s) 854, 867, 1026
MslI CAYNNNNRTG 6 cut(s) 600, 1296, 1852, 2194, 2355, 2632
MspA1I CMGCKG 3 cut(s) 156, 980, 2304
MspCI CTTAAG 1 cut(s) 2210
MspI CCGG 2 cut(s) 94, 2907
MspR9I CCNGG 4 cut(s) 292, 1884, 2907, 2993
Mva1269I GAATGC 1 cut(s) 1765
MvaI CCWGG 3 cut(s) 292, 1884, 2993
MwoI GCNNNNNNNGC 6 cut(s) 64, 910, 974, 1124, 1959, 2186
NciI CCSGG 1 cut(s) 2907
NdeI CATATG 1 cut(s) 792
NdeII GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
NheI GCTAGC 1 cut(s) 2327
NlaIV GGNNCC 6 cut(s) 98, 525, 713, 1029, 1619, 2859
NmeAIII GCCGAG 1 cut(s) 2404
NmuCI GTSAC 3 cut(s) 2281, 2492, 2755
NsbI TGCGCA 1 cut(s) 2051
NsiI ATGCAT 5 cut(s) 412, 970, 1968, 2491, 2639
NspI RCATGY 4 cut(s) 662, 1966, 2493, 2991
OliI CACNNNNGTG 1 cut(s) 2355
PaeI GCATGC 2 cut(s) 1966, 2991
PaqCI CACCTGC 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 765
PctI GAATGC 1 cut(s) 1765
PdmI GAANNNNTTC 3 cut(s) 854, 867, 1026
PfeI GAWTC 7 cut(s) 61, 146, 185, 1100, 1663, 1692, 2878
PflMI CCANNNNNTGG 1 cut(s) 2526
PkrI GCNGC 7 cut(s) 158, 493, 979, 982, 1876, 2303, 2370
PleI GAGTC 5 cut(s) 1282, 1298, 1778, 2223, 2645
PpsI GAGTC 5 cut(s) 1282, 1298, 1778, 2223, 2645
Ppu21I YACGTR 3 cut(s) 53, 165, 227
PshBI ATTAAT 1 cut(s) 1527
PsiI TTATAA 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 2779
Psp6I CCWGG 3 cut(s) 290, 1882, 2991
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 3 cut(s) 290, 1882, 2991
PspN4I GGNNCC 6 cut(s) 98, 525, 713, 1029, 1619, 2859
PspPI GGNCC 5 cut(s) 158, 346, 712, 2309, 2540
PstI CTGCAG 3 cut(s) 979, 2370, 2501
PvuII CAGCTG 2 cut(s) 980, 2304
RsaI GTAC 4 cut(s) 1557, 1614, 2293, 2911
RsaNI GTAC 4 cut(s) 1556, 1613, 2292, 2910
RseI CAYNNNNRTG 6 cut(s) 600, 1296, 1852, 2194, 2355, 2632
SacI GAGCTC 1 cut(s) 2779
SapI GCTCTTC 1 cut(s) 765
SatI GCNGC 7 cut(s) 157, 492, 978, 981, 1875, 2302, 2369
Sau3AI GATC 8 cut(s) 628, 646, 651, 918, 952, 2418, 2461, 2728
Sau96I GGNCC 5 cut(s) 158, 346, 712, 2309, 2540
SchI GAGTC 5 cut(s) 1283, 1298, 1778, 2224, 2645
ScrFI CCNGG 4 cut(s) 292, 1884, 2907, 2993
SduI GDGCHC 3 cut(s) 313, 2779, 3021
SfaNI GCATC 6 cut(s) 566, 826, 977, 2140, 2636, 2665
SfcI CTRYAG 5 cut(s) 975, 1976, 2366, 2497, 2972
SinI GGWCC 3 cut(s) 346, 2309, 2540
SmiMI CAYNNNNRTG 6 cut(s) 600, 1296, 1852, 2194, 2355, 2632
SmlI CTYRAG 3 cut(s) 352, 548, 2210
SmoI CTYRAG 3 cut(s) 352, 548, 2210
SnaBI TACGTA 1 cut(s) 165
SpeI ACTAGT 1 cut(s) 2737
SphI GCATGC 2 cut(s) 1966, 2991
SsiI CCGC 4 cut(s) 156, 1938, 2264, 2342
SspI AATATT 1 cut(s) 925
SspMI CTAG 6 cut(s) 417, 1470, 1689, 2328, 2706, 2738
SstI GAGCTC 1 cut(s) 2779
StyD4I CCNGG 4 cut(s) 290, 1882, 2905, 2991
StyI CCWWGG 1 cut(s) 742
TaaI ACNGT 7 cut(s) 499, 750, 1381, 2404, 2660, 2976, 3016
TaiI ACGT 8 cut(s) 55, 167, 229, 1040, 1341, 1780, 1794, 2397
TaqI TCGA 8 cut(s) 248, 958, 2148, 2319, 2505, 2545, 2850, 2916
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 7 cut(s) 61, 146, 185, 1100, 1663, 1692, 2878
TscAI CASTG 7 cut(s) 755, 1189, 1548, 2343, 2501, 2665, 2805
TseFI GTSAC 3 cut(s) 2281, 2492, 2755
TseI GCWGC 6 cut(s) 491, 977, 980, 1874, 2301, 2368
Tsp45I GTSAC 3 cut(s) 2281, 2492, 2755
TspRI CASTG 7 cut(s) 755, 1189, 1548, 2343, 2501, 2665, 2805
Van91I CCANNNNNTGG 1 cut(s) 2526
Vha464I CTTAAG 1 cut(s) 2210
VneI GTGCAC 1 cut(s) 309
VpaK11BI GGWCC 3 cut(s) 346, 2309, 2540
VspI ATTAAT 1 cut(s) 1527
XapI RAATTY 5 cut(s) 109, 1164, 1649, 2376, 2587
XceI RCATGY 4 cut(s) 662, 1966, 2493, 2991
XcmI CCANNNNNNNNNTGG 2 cut(s) 740, 2527
XmiI GTMKAC 3 cut(s) 167, 1277, 2971
XmnI GAANNNNTTC 3 cut(s) 854, 867, 1026
XspI CTAG 6 cut(s) 417, 1470, 1689, 2328, 2706, 2738
Zsp2I ATGCAT 5 cut(s) 412, 970, 1968, 2491, 2639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.