MD14G1040700.v1.1

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010066
Physical Location & Seq
Forward (+)
3834351 .. 3847676
13326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1040700.v1.1.491

Sequence Viewer

Length: 3897 bp
ATGGTGGATCCGGCGAATACGCCACTTGGCAGAATGCTATTGGAGGAGATCACTCCCATTGTCATGGTGCTCCGTACGCCCCTCGTCGAAGAAGCCTGCATCAAGAACGGCCTCACCTTAGTCCAAATACTCAAGCCCTTCTGCGTCTTCAACAACATCGACGTGCCTGTGCGGACTGCGAGTGATCAACCCTACAGGCTTCAGAAGTTCGGATTGCGGTTGTTTTATGAGTCGGATATTCGGCAACCGAATTTGGAGGTAGCAAAAGAGAGGTTGAAACAAGTCATAAACCAGGCGGCGGATAAAGATCTTTCTGAATTGTGCTCAGACCTGCCTCAGATTGATAATGCATTATCCATATCGGAATCTGAAGTTCTACCATCATGGTTTCAGTTTTTCAACAAAGAGCTTATGCATAGCGTCTCTTTTTCAGACCATGAAGCTTTTGATCATCCTGTGGCATGTCTCGTTGTAGTTTCTTCAAAGGATGATCAACCTATCAACAGATTTGTTGACCTCTTTAACACCAAGAAGTTGCCCTCCCTTCTTACTAATGGGGCAATGGACCCAAAGATTTTGAAGCATTATTTACTAGTGCATGATAAACAAGATGGCCCTTTAGAGAAGGCAACTAAAATTTTAACTGAAATGAGGAGCACTTTTGGTTCTGACTGTCAGTTACTATGCATTAATTCTTCTCAAGATGGTGTGGTTGAACATCAAGATTATCCCTGGGTGCTTTATAAATTTGAAGATTTACCTAGTCAACCGCTTCGTTGCTTCCTTAATATTGAGGATATTAATGGGATAAAAGACCTGATGCAAGATTTATCAACTAAACACATTATTCCCTACATGGAGCAAAAAATACGTTTGCTCAATCAGCAGGCATGTATCTTGACAATATTTCCTTCAGTTGCTGCAACAAGGAAAGGTTTTAGGAACCAAATAAAAAATCTATGGTGGAGAAAAGGAAAAGATGATGTGGTGGATTCTCCCAGTGGCCCTACGTACACCTTTAACTCCATTGAATCCCAAATTAGAGTTTTGGGTGATTATGCCTTCATGCTACGTGATTATGAACTTGCATTGTCGAATTATCGCCTAATATCCACAGATTACAAGCTTGACAAAGCCTGGAAACGCTATGCTGGCGTACAGGAAATGATGGGGCTTGCATATTTCATGTCAGACCAGTCAAGAAAGGATGCAGAGTATTGCATGGAAAACGCATTTACTACCTATTTAAAAGTTGCACCATCTAGCCAGCAAAACGCAACTAGGTGTGGTCTTTGGTGGGTGGAGATGTTGAAGGCACGACATCAGTATAAAGAGGCTGCAACTGTTTACTTCCGTGTTTGTACGGAGGAGCCACTATATTCCGCTGTAATGCTTGAGCAAGCGTCTTATTGTTACTTGCTGTCTAGACCACCTATGTTGCACAAATATGGATTTCATCTTGTTCTTTCTGGTGACCGTTACAAGAAATCTGATCAGGTCAAACATGCAATTCGCACATATAGAGGTGCCATGTCTGTGTATACAGGAACTACGTGGAGCCATATCAAAGACCATGTTCATTTCCATATTGGACAGTGGTATGCTCTTCTTGGGCTATATGATTTGGCCGCAAATCATGTGATGGAGGTCCTGGCCTGTAGTCATCAGTCCAAGAAAACGCAGGAGTTATTCCTGAGAGACTTCCTTCAAATTGTTCAGAAAACAGGGAAAACATTTGAAGTATCAAAGCTTCAATTGCCGGAGATCAATATTTCATCTCTTAGGGTAATTTTTGAGGATCATCGGACCTATGCATCATCTGCAGCTGCAAGTGTCAAGGAAAGAATATGGGTCTCCTTAGAGGAGGAAATGATTCCAAATTTGTCCACCGCCAGAACTAATTGGTTGGAGTTACAGTCAAAGCTTATCCCAAAGAAGTACAAAGAGTCAAATGTTTGCGTTGCAGGAGAAGCTGTCAGGGTTGATATTGAATTAAAAAATCCGTTGCAAATCCCCCTCCCCTTGTCTAGTGTTTCCCTATTATGCGAGCTTTCTGCTGGCTCTGATGAAATGAAATCCGATGCAAGTAGCTCGTTAGCGGAGATTCAGGATGGAGAATCTACTAGTTTAATTCATAGGGATGTGAACTTTGAGAGTTCTTTATTTTCTTTGTCTGATGTTGACTTTTCATTAGCAGGAGGTGAAACAATTGTGGTGCAACTAACGGTTACTCCTAGAGTAGAAGGCATTTTACAGATTGTTGGTGTAAAGTGGAAATTGTCAGGCTCTGTTGTTGGCTTCCATAAATTTGACACTAACCCCTTGAAGAAGATATGCAGAAAGCAAATACAGAAGGCGAAGCATCCTCATTGTGATAATTTAAAGTTTGTAGTTGTTAAGAGTGTACCAAAGCTTGAGGGTGTCATTCATCCACTACCTAAAAGGGCATATACAGGAGATTTACGGCATCTTGTGTTGGAGTTGAAGAACAAATCTGAATTTGCTGTGAAGAATCTGAAAATGAATATTAGTCATCCTAGATTCTTGAATCTTGGAAAGCGAGAAAGTTTGAACACCGAGTTTCCTGCTTGCTTAGAAAAGAAAAGTTCTGATCAGTCTGCTGAACATGCTAACCTTAATGATGTTTCTCATGGCCTGTTTCTATTTCCTGAGGACACTATTATTCAAGGGGAAACACCATTATTGTGGCCTCTTTGGTTTCGGGCAGCTGTTCCTGGGAACATTTCCTTGTACATAACAATATATTATGAGATGAGTGATATTTCAAGCACCATGAGATTTCGCACTTTACGCATGCATTACAATTTGCAGGTATTGCCGTCATTGGACGTGTCATTCCTTATCAGTCCTTGTCCATCAAGATTGCAAGAGTTTCTTGTGCGCATGGATGTTGTCAACAAGACAAGCTCTGAAAGTTTCCAAATTCATCAGTTGTCATCTGTTGGGAACCAATGGGAAATCTCACTACTTCAACCTGTTGATGCCATCTTTCCTTCACAATCTTTAACACCTCATCAAGCATTGTCATGCTTCTTCAGGCTCAAGAGTTGTGGCAAGTCATCAACTTCTGAAGACGAGAAATCTTCCCATTCCCGACTTCAGGGAACTGATTTGAGATTGGGTACTCAGGGCAGTAATGGACCACGTTTTGATATAGCTAGCTCTCCCCTGGCAGATTTCCACCGCTCTGAAAGACTGCACCAGGAAGTTTTAAATAAGGGAGACACTAATCCGGTAGACTTCATATTGATTTCCCGGCCTCTGAAGAATGACATTAATCCTGAGGTTTCTGAGCCACCGCATCTTTTTTCTCATCATGCATGTTACTGCAGTACTGCGACCACAAGTCCTATATCATGGCTAGTGGACGGACCTCGAACCTTATACCATAACTTCTCTGCTTCTTTTTGTGAAATAAATCTTAGCATGACTATATACAACGCATCGGATGTGGTTGCATCTGTACGCATAAACACCTCGGACTCCAGTACTAGCGATCATTTAAGTGATGCAACTCCAGTTCTACCTGCGACGTCTTCTGGCAACCAAGATGGATGGCATGACCTCTCACCAGTGACTGACATCAAAGTGACTTCCGATGCCCTTGGATCCCGGGCCAGTAAGTCAATTCCCGTGGAGAGCGTCTCCCCATTTATTTGGTCCGGGTCAAGCTCGACTAGAGTCCAACTTGATCCCATGTCAAGAACTGAAATTCCTCTTCAAGTTTGTGTATTCTCTCCCGGGACATATGATCTGTCGAGCTATCTTTTGCATTGGAATCTTTTACTTTCTAATGATCAAGAAAATCGGGATAGGTCGTCTTCTGGGACGTGCCAGGGATATCCGTATTACCTTACTGTACTCCAGTCGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPPC-Trs85 TRAPPC9-Trs120

Protein Analysis

1299

Amino Acids

146.7

Weight (kDa)

6.31

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC-Trs85 PF12739 16 - 440 9.6e-82 ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Ig_TPPC8_1st PF24545 614 - 766 5e-15 TPPC8 first Ig-like domain
Ig_TPPC8_2nd PF24544 819 - 937 3.6e-06 TPPC8 second Ig-like domain
Ig_TPPC8_C PF24542 1212 - 1261 1.8e-07 TPPC8 C-terminal Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16280 AT5G16280
fragaria_vesca FvH4_6g20450 FvH4_6g20450 FvH4_6g20450 FvH4_6g20450
malus_domestica MD12G1041600.v1.1 MD14G1040700.v1.1
prunus_persica Prupe.7G093600_v2.0.a1
pyrus_communis pycom12g03630 pycom14g03450
rosa_chinensis RchiOBHm_Chr3g0476301
rosa_laevigata RLG00000023780
rosa_multiflora Rmu_sc0000114.1_g000012
rosa_roxburghii Rroxscaffold_6G00405410
rosa_rugosa Rorug03G0153400
rosa_samantha Rh3AG204600 Rh3BG236000 Rh3CG230700 Rh3DG230500
rosa_wichuraiana Rw3G018590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AatII GACGTC 1 cut(s) 3557
Acc16I TGCGCA 1 cut(s) 2903
Acc36I ACCTGC 3 cut(s) 339, 2821, 3556
AccB1I GGYRCC 1 cut(s) 1526
AccBSI CCGCTC 1 cut(s) 3207
AccI GTMKAC 2 cut(s) 1541, 3258
AclWI GGATC 6 cut(s) 2, 15, 1806, 3624, 3637, 3707
AcoI YGGCCR 1 cut(s) 1626
AcsI RAATTY 8 cut(s) 250, 636, 746, 1879, 2306, 2498, 2943, 3732
AcuI CTGAAG 7 cut(s) 185, 390, 897, 3040, 3104, 3111, 3305
AcyI GRCGYC 1 cut(s) 3554
AfiI CCNNNNNNNGG 2 cut(s) 298, 3121
AflIII ACRYGT 1 cut(s) 2850
AhdI GACNNNNNGTC 1 cut(s) 3366
AhlI ACTAGT 2 cut(s) 592, 2123
AjiI CACGTC 3 cut(s) 163, 2851, 3852
AjnI CCWGG 8 cut(s) 291, 731, 1136, 1650, 2734, 3189, 3222, 3855
AloI GAACNNNNNNTCC 2 cut(s) 3718, 3750
Alw21I GWGCWC 3 cut(s) 72, 326, 659
Alw26I GTCTC 6 cut(s) 427, 470, 1692, 1858, 3237, 3670
AlwI GGATC 6 cut(s) 2, 15, 1806, 3624, 3637, 3707
AlwNI CAGNNNCTG 3 cut(s) 920, 2288, 3599
Ama87I CYCGRG 2 cut(s) 3633, 3761
AoxI GGCC 9 cut(s) 109, 613, 1003, 1626, 1653, 2653, 2708, 3278, 3636
ApeKI GCWGC 5 cut(s) 920, 1337, 1823, 1826, 2726
ApoI RAATTY 8 cut(s) 250, 636, 746, 1879, 2306, 2498, 2943, 3732
AseI ATTAAT 3 cut(s) 690, 801, 3297
Asp700I GAANNNNTTC 1 cut(s) 1872
AspLEI GCGC 1 cut(s) 2904
AspS9I GGNCC 9 cut(s) 565, 614, 1004, 1648, 1806, 3161, 3392, 3636, 3681
AsuC2I CCSGG 6 cut(s) 3277, 3634, 3635, 3685, 3762, 3763
AsuHPI GGTGA 5 cut(s) 106, 1064, 1484, 2213, 3582
AsuNHI GCTAGC 1 cut(s) 3179
AvaI CYCGRG 2 cut(s) 3633, 3761
AvaII GGWCC 6 cut(s) 565, 1648, 1806, 3161, 3392, 3681
AxyI CCTNAGG 2 cut(s) 2670, 3303
BamHI GGATCC 2 cut(s) 7, 3629
BanI GGYRCC 1 cut(s) 1526
BarI GAAGNNNNNNTAC 4 cut(s) 1734, 1766, 2235, 2267
BbsI GAAGAC 4 cut(s) 139, 3099, 3549, 3834
Bbv12I GWGCWC 3 cut(s) 72, 326, 659
BbvI GCAGC 5 cut(s) 907, 1324, 1813, 1835, 2738
BceAI ACGGC 3 cut(s) 124, 2480, 2824
BcgI CGANNNNNNTGC 6 cut(s) 2036, 2070, 2073, 2107, 2567, 2601
BciT130I CCWGG 8 cut(s) 293, 733, 1138, 1652, 2736, 3191, 3224, 3857
BclI TGATCA 6 cut(s) 184, 448, 490, 1492, 2611, 3817
BcnI CCSGG 6 cut(s) 3277, 3634, 3635, 3685, 3762, 3763
BcoDI GTCTC 6 cut(s) 427, 470, 1692, 1858, 3237, 3670
BcuI ACTAGT 2 cut(s) 592, 2123
BfmI CTRYAG 4 cut(s) 193, 1657, 1821, 3349
BfuAI ACCTGC 3 cut(s) 339, 2821, 3556
BglII AGATCT 1 cut(s) 307
BisI GCNGC 7 cut(s) 297, 921, 1338, 1629, 1824, 1827, 2727
BlsI GCNGC 7 cut(s) 298, 922, 1339, 1630, 1825, 1828, 2728
BmcAI AGTACT 2 cut(s) 3355, 3511
Bme18I GGWCC 6 cut(s) 565, 1648, 1806, 3161, 3392, 3681
BmeRI GACNNNNNGTC 1 cut(s) 3366
BmeT110I CYCGRG 2 cut(s) 3633, 3761
BmgBI CACGTC 3 cut(s) 163, 2851, 3852
BmgT120I GGNCC 9 cut(s) 565, 614, 1004, 1648, 1806, 3161, 3392, 3636, 3681
BmiI GGNNCC 8 cut(s) 9, 567, 944, 1371, 1528, 1559, 2969, 3631
BmrI ACTGGG 1 cut(s) 993
BmtI GCTAGC 1 cut(s) 3183
BmuI ACTGGG 1 cut(s) 993
BoxI GACNNNNGTC 1 cut(s) 3701
BpiI GAAGAC 4 cut(s) 139, 3099, 3549, 3834
BplI GAGNNNNNCTC 2 cut(s) 3650, 3682
BpmI CTGGAG 3 cut(s) 3490, 3522, 3869
BpuEI CTTGAG 5 cut(s) 116, 684, 1415, 2435, 3047
BpuMI CCSGG 6 cut(s) 3277, 3634, 3635, 3685, 3762, 3763
BsaAI YACGTR 3 cut(s) 1011, 1073, 1554
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 3554
BsaI GGTCTC 1 cut(s) 1858
BsaWI WCCGGW 1 cut(s) 3253
BsaXI ACNNNNNCTCC 6 cut(s) 1901, 1931, 1959, 1989, 2215, 2245
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 3121
Bse1I ACTGG 7 cut(s) 999, 1195, 3507, 3539, 3593, 3639, 3886
Bse21I CCTNAGG 2 cut(s) 2670, 3303
Bse3DI GCAATG 1 cut(s) 567
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 8 cut(s) 293, 733, 1138, 1652, 2736, 3191, 3224, 3857
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 298, 3121
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 7 cut(s) 339, 350, 1685, 2661, 3161, 3294, 3303
BseNI ACTGG 7 cut(s) 999, 1195, 3507, 3539, 3593, 3639, 3886
BseRI GAGGAG 4 cut(s) 59, 667, 1382, 1877
BseXI GCAGC 5 cut(s) 907, 1324, 1813, 1835, 2738
BsgI GTGCAG 1 cut(s) 3203
BshFI GGCC 9 cut(s) 111, 615, 1005, 1628, 1655, 2655, 2710, 3280, 3638
BshNI GGYRCC 1 cut(s) 1526
BsiHKAI GWGCWC 3 cut(s) 72, 326, 659
BsiHKCI CYCGRG 2 cut(s) 3633, 3761
BsiSI CCGG 7 cut(s) 11, 1760, 3254, 3277, 3634, 3684, 3762
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 3778, 3862
BslI CCNNNNNNNGG 2 cut(s) 298, 3121
BsmAI GTCTC 6 cut(s) 427, 470, 1692, 1858, 3237, 3670
BsmBI CGTCTC 2 cut(s) 427, 3670
BsmFI GGGAC 2 cut(s) 3778, 3862
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 9 cut(s) 111, 615, 1005, 1628, 1655, 2655, 2710, 3280, 3638
Bso31I GGTCTC 1 cut(s) 1858
BsoBI CYCGRG 2 cut(s) 3633, 3761
Bsp1286I GDGCHC 3 cut(s) 72, 326, 659
Bsp1407I TGTACA 1 cut(s) 2751
BspANI GGCC 9 cut(s) 111, 615, 1005, 1628, 1655, 2655, 2710, 3280, 3638
BspCNI CTCAG 7 cut(s) 338, 349, 1686, 2662, 3160, 3295, 3304
BspLI GGNNCC 8 cut(s) 9, 567, 944, 1371, 1528, 1559, 2969, 3631
BspMAI CTGCAG 2 cut(s) 1825, 3353
BspMI ACCTGC 3 cut(s) 339, 2821, 3556
BspOI GCTAGC 1 cut(s) 3183
BspPI GGATC 6 cut(s) 2, 15, 1806, 3624, 3637, 3707
BspQI GCTCTTC 1 cut(s) 1611
BspT107I GGYRCC 1 cut(s) 1526
BspTNI GGTCTC 1 cut(s) 1858
BsrBI CCGCTC 1 cut(s) 3207
BsrDI GCAATG 1 cut(s) 567
BsrGI TGTACA 1 cut(s) 2751
BsrI ACTGG 7 cut(s) 999, 1195, 3507, 3539, 3593, 3639, 3886
BssNAI GTATAC 1 cut(s) 1542
BssNI GRCGYC 1 cut(s) 3554
BssT1I CCWWGG 1 cut(s) 3625
Bst1107I GTATAC 1 cut(s) 1542
Bst2UI CCWGG 8 cut(s) 293, 733, 1138, 1652, 2736, 3191, 3224, 3857
Bst4CI ACNGT 7 cut(s) 674, 1345, 1478, 1596, 1917, 2227, 3880
Bst6I CTCTTC 2 cut(s) 1611, 3744
BstACI GRCGYC 1 cut(s) 3554
BstAPI GCANNNNNTGC 2 cut(s) 1820, 2836
BstAUI TGTACA 1 cut(s) 2751
BstBAI YACGTR 3 cut(s) 1011, 1073, 1554
BstDSI CCRYGG 1 cut(s) 3654
BstEII GGTNACC 1 cut(s) 1472
BstHHI GCGC 1 cut(s) 2904
BstMAI GTCTC 6 cut(s) 427, 470, 1692, 1858, 3237, 3670
BstMWI GCNNNNNNNGC 9 cut(s) 76, 883, 1152, 1756, 1820, 1970, 2627, 2811, 2836
BstNI CCWGG 8 cut(s) 293, 733, 1138, 1652, 2736, 3191, 3224, 3857
BstNSI RCATGY 6 cut(s) 465, 894, 1508, 2630, 2818, 3345
BstPAI GACNNNNGTC 1 cut(s) 3701
BstPI GGTNACC 1 cut(s) 1472
BstSFI CTRYAG 4 cut(s) 193, 1657, 1821, 3349
BstSNI TACGTA 1 cut(s) 1011
BstV1I GCAGC 5 cut(s) 907, 1324, 1813, 1835, 2738
BstV2I GAAGAC 4 cut(s) 139, 3099, 3549, 3834
BstX2I RGATCY 3 cut(s) 7, 307, 3629
BstXI CCANNNNNNTGG 3 cut(s) 64, 2706, 3678
BstYI RGATCY 3 cut(s) 7, 307, 3629
BstZ17I GTATAC 1 cut(s) 1542
Bsu36I CCTNAGG 2 cut(s) 2670, 3303
BsuRI GGCC 9 cut(s) 111, 615, 1005, 1628, 1655, 2655, 2710, 3280, 3638
BtgI CCRYGG 1 cut(s) 3654
BtrI CACGTC 3 cut(s) 163, 2851, 3852
BtsIMutI CAGTG 3 cut(s) 1006, 1601, 3600
BveI ACCTGC 3 cut(s) 339, 2821, 3556
CaiI CAGNNNCTG 3 cut(s) 920, 2288, 3599
CfoI GCGC 1 cut(s) 2904
Cfr13I GGNCC 9 cut(s) 565, 614, 1004, 1648, 1806, 3161, 3392, 3636, 3681
Cfr9I CCCGGG 2 cut(s) 3633, 3761
CseI GACGC 4 cut(s) 133, 409, 1392, 3652
CspCI CAANNNNNGTGG 6 cut(s) 1419, 1454, 3052, 3087, 3636, 3671
DraI TTTAAA 3 cut(s) 1248, 2382, 3234
DriI GACNNNNNGTC 1 cut(s) 3366
EaeI YGGCCR 1 cut(s) 1626
Eam1104I CTCTTC 2 cut(s) 1611, 3744
Eam1105I GACNNNNNGTC 1 cut(s) 3366
EarI CTCTTC 2 cut(s) 1611, 3744
EciI GGCGGA 1 cut(s) 314
Eco105I TACGTA 1 cut(s) 1011
Eco130I CCWWGG 1 cut(s) 3625
Eco31I GGTCTC 1 cut(s) 1858
Eco32I GATATC 1 cut(s) 3863
Eco47I GGWCC 6 cut(s) 565, 1648, 1806, 3161, 3392, 3681
Eco57I CTGAAG 7 cut(s) 185, 390, 897, 3040, 3104, 3111, 3305
Eco81I CCTNAGG 2 cut(s) 2670, 3303
Eco88I CYCGRG 2 cut(s) 3633, 3761
Eco91I GGTNACC 1 cut(s) 1472
EcoO109I RGGNCCY 1 cut(s) 1648
EcoO65I GGTNACC 1 cut(s) 1472
EcoRII CCWGG 8 cut(s) 291, 731, 1136, 1650, 2734, 3189, 3222, 3855
EcoRV GATATC 1 cut(s) 3863
EcoT14I CCWWGG 1 cut(s) 3625
EcoT22I ATGCAT 6 cut(s) 352, 417, 689, 1816, 2820, 3343
ErhI CCWWGG 1 cut(s) 3625
Esp3I CGTCTC 2 cut(s) 427, 3670
FalI AAGNNNNNCTT 2 cut(s) 2880, 2912
FaqI GGGAC 2 cut(s) 3778, 3862
FauNDI CATATG 1 cut(s) 3769
FbaI TGATCA 6 cut(s) 184, 448, 490, 1492, 2611, 3817
FblI GTMKAC 2 cut(s) 1541, 3258
Fnu4HI GCNGC 7 cut(s) 297, 921, 1338, 1629, 1824, 1827, 2727
Fsp4HI GCNGC 7 cut(s) 297, 921, 1338, 1629, 1824, 1827, 2727
FspAI RTGCGCAY 1 cut(s) 2903
FspI TGCGCA 1 cut(s) 2903
GlaI GCGC 1 cut(s) 2903
GluI GCNGC 7 cut(s) 297, 921, 1338, 1629, 1824, 1827, 2727
GsuI CTGGAG 3 cut(s) 3490, 3522, 3869
HaeIII GGCC 9 cut(s) 111, 615, 1005, 1628, 1655, 2655, 2710, 3280, 3638
HapII CCGG 7 cut(s) 11, 1760, 3254, 3277, 3634, 3684, 3762
HgaI GACGC 4 cut(s) 133, 409, 1392, 3652
HhaI GCGC 1 cut(s) 2904
Hin1I GRCGYC 1 cut(s) 3554
Hin6I GCGC 1 cut(s) 2902
HinP1I GCGC 1 cut(s) 2902
HincII GTYRAC 4 cut(s) 514, 767, 2182, 2917
HindII GTYRAC 4 cut(s) 514, 767, 2182, 2917
HindIII AAGCTT 5 cut(s) 441, 1124, 1748, 1922, 2411
HpaII CCGG 7 cut(s) 11, 1760, 3254, 3277, 3634, 3684, 3762
HphI GGTGA 5 cut(s) 106, 1064, 1484, 2213, 3582
Hpy99I CGWCG 3 cut(s) 89, 164, 3556
HpyCH4III ACNGT 7 cut(s) 674, 1345, 1478, 1596, 1917, 2227, 3880
HpyCH4IV ACGT 9 cut(s) 162, 871, 1010, 1072, 1553, 2850, 3166, 3554, 3851
HpyF10VI GCNNNNNNNGC 9 cut(s) 76, 883, 1152, 1756, 1820, 1970, 2627, 2811, 2836
HpySE526I ACGT 9 cut(s) 162, 871, 1010, 1072, 1553, 2850, 3166, 3554, 3851
Hsp92I GRCGYC 1 cut(s) 3554
HspAI GCGC 1 cut(s) 2902
Ksp22I TGATCA 6 cut(s) 184, 448, 490, 1492, 2611, 3817
LguI GCTCTTC 1 cut(s) 1611
LmnI GCTCC 5 cut(s) 75, 654, 859, 1369, 1557
Lsp1109I GCAGC 5 cut(s) 907, 1324, 1813, 1835, 2738
MaeII ACGT 9 cut(s) 162, 871, 1010, 1072, 1553, 2850, 3166, 3554, 3851
MaeIII GTNAC 9 cut(s) 678, 1412, 1472, 1478, 1911, 2227, 3344, 3595, 3610
MbiI CCGCTC 1 cut(s) 3207
MfeI CAATTG 2 cut(s) 1754, 2208
MflI RGATCY 3 cut(s) 7, 307, 3629
MhlI GDGCHC 3 cut(s) 72, 326, 659
MlyI GAGTC 4 cut(s) 239, 1955, 3497, 3711
MmeI TCCRAC 5 cut(s) 213, 1887, 2457, 3730, 3870
Mph1103I ATGCAT 6 cut(s) 352, 417, 689, 1816, 2820, 3343
MroXI GAANNNNTTC 1 cut(s) 1872
MslI CAYNNNNRTG 9 cut(s) 62, 161, 1446, 1535, 2139, 2704, 3046, 3525, 3608
MspA1I CMGCKG 3 cut(s) 1385, 1826, 2729
MspI CCGG 7 cut(s) 11, 1760, 3254, 3277, 3634, 3684, 3762
MunI CAATTG 2 cut(s) 1754, 2208
Mva1269I GAATGC 1 cut(s) 39
MvaI CCWGG 8 cut(s) 293, 733, 1138, 1652, 2736, 3191, 3224, 3857
MwoI GCNNNNNNNGC 9 cut(s) 76, 883, 1152, 1756, 1820, 1970, 2627, 2811, 2836
NciI CCSGG 6 cut(s) 3277, 3634, 3635, 3685, 3762, 3763
NdeI CATATG 1 cut(s) 3769
NheI GCTAGC 1 cut(s) 3179
NlaIV GGNNCC 8 cut(s) 9, 567, 944, 1371, 1528, 1559, 2969, 3631
NmuCI GTSAC 3 cut(s) 1472, 3595, 3610
NsbI TGCGCA 1 cut(s) 2903
NsiI ATGCAT 6 cut(s) 352, 417, 689, 1816, 2820, 3343
NspI RCATGY 6 cut(s) 465, 894, 1508, 2630, 2818, 3345
PaeI GCATGC 1 cut(s) 2818
PasI CCCWGGG 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 1611
PctI GAATGC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 1872
Pfl23II CGTACG 1 cut(s) 74
PkrI GCNGC 7 cut(s) 298, 922, 1339, 1630, 1825, 1828, 2728
PleI GAGTC 4 cut(s) 238, 1954, 3497, 3710
PpsI GAGTC 4 cut(s) 238, 1954, 3497, 3710
Ppu21I YACGTR 3 cut(s) 1011, 1073, 1554
PpuMI RGGWCCY 1 cut(s) 1648
PshAI GACNNNNGTC 1 cut(s) 3701
PshBI ATTAAT 3 cut(s) 690, 801, 3297
PsiI TTATAA 1 cut(s) 744
Psp5II RGGWCCY 1 cut(s) 1648
Psp6I CCWGG 8 cut(s) 291, 731, 1136, 1650, 2734, 3189, 3222, 3855
PspEI GGTNACC 1 cut(s) 1472
PspGI CCWGG 8 cut(s) 291, 731, 1136, 1650, 2734, 3189, 3222, 3855
PspLI CGTACG 1 cut(s) 74
PspN4I GGNNCC 8 cut(s) 9, 567, 944, 1371, 1528, 1559, 2969, 3631
PspPI GGNCC 9 cut(s) 565, 614, 1004, 1648, 1806, 3161, 3392, 3636, 3681
PspPPI RGGWCCY 1 cut(s) 1648
PstI CTGCAG 2 cut(s) 1825, 3353
PstNI CAGNNNCTG 3 cut(s) 920, 2288, 3599
PsuI RGATCY 3 cut(s) 7, 307, 3629
PvuII CAGCTG 2 cut(s) 1826, 2729
RseI CAYNNNNRTG 9 cut(s) 62, 161, 1446, 1535, 2139, 2704, 3046, 3525, 3608
SapI GCTCTTC 1 cut(s) 1611
SatI GCNGC 7 cut(s) 297, 921, 1338, 1629, 1824, 1827, 2727
Sau96I GGNCC 9 cut(s) 565, 614, 1004, 1648, 1806, 3161, 3392, 3636, 3681
ScaI AGTACT 2 cut(s) 3355, 3511
SchI GAGTC 4 cut(s) 239, 1955, 3497, 3711
SduI GDGCHC 3 cut(s) 72, 326, 659
SfcI CTRYAG 4 cut(s) 193, 1657, 1821, 3349
SinI GGWCC 6 cut(s) 565, 1648, 1806, 3161, 3392, 3681
SmaI CCCGGG 2 cut(s) 3635, 3763
SmiMI CAYNNNNRTG 9 cut(s) 62, 161, 1446, 1535, 2139, 2704, 3046, 3525, 3608
SmlI CTYRAG 5 cut(s) 131, 699, 1394, 2414, 3062
SmoI CTYRAG 5 cut(s) 131, 699, 1394, 2414, 3062
SnaBI TACGTA 1 cut(s) 1011
SpeI ACTAGT 2 cut(s) 592, 2123
SphI GCATGC 1 cut(s) 2818
SspI AATATT 4 cut(s) 790, 906, 1771, 2527
StyI CCWWGG 1 cut(s) 3625
TaaI ACNGT 7 cut(s) 674, 1345, 1478, 1596, 1917, 2227, 3880
TaiI ACGT 9 cut(s) 165, 874, 1013, 1075, 1556, 2853, 3169, 3557, 3854
TaqI TCGA 6 cut(s) 87, 159, 1094, 3397, 3695, 3779
TatI WGTACW 5 cut(s) 1938, 2751, 3353, 3509, 3880
TauI GCSGC 2 cut(s) 299, 1631
TscAI CASTG 3 cut(s) 1006, 1601, 3600
TseFI GTSAC 3 cut(s) 1472, 3595, 3610
TseI GCWGC 5 cut(s) 920, 1337, 1823, 1826, 2726
Tsp45I GTSAC 3 cut(s) 1472, 3595, 3610
TspGWI ACGGA 6 cut(s) 62, 1343, 1379, 1992, 3405, 3855
TspMI CCCGGG 2 cut(s) 3633, 3761
TspRI CASTG 3 cut(s) 1006, 1601, 3600
VpaK11BI GGWCC 6 cut(s) 565, 1648, 1806, 3161, 3392, 3681
VspI ATTAAT 3 cut(s) 690, 801, 3297
XapI RAATTY 8 cut(s) 250, 636, 746, 1879, 2306, 2498, 2943, 3732
XbaI TCTAGA 1 cut(s) 1424
XceI RCATGY 6 cut(s) 465, 894, 1508, 2630, 2818, 3345
XmaI CCCGGG 2 cut(s) 3633, 3761
XmiI GTMKAC 2 cut(s) 1541, 3258
XmnI GAANNNNTTC 1 cut(s) 1872
ZraI GACGTC 1 cut(s) 3555
ZrmI AGTACT 2 cut(s) 3355, 3511
Zsp2I ATGCAT 6 cut(s) 352, 417, 689, 1816, 2820, 3343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.