MD12G1041600.v1.1

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010066
Physical Location & Seq
Forward (+)
4609109 .. 4630798
21690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041600.v1.1.491

Sequence Viewer

Length: 3858 bp
ATGGTGGATCCGGCGAATACGCCACTTGGCAGAATGTTATTGGAGGAGATCACTCCCGTCATCATGGTCCTCCGTACGCCCCTCGTCGAAGAAGCTTGCCTCAAGAACGGCCTCACCTTCGTTCAAATGCTCAAGCCCTTCTGCGTCTTCAACAACATCGACGTGCCGGTGCGGACTGCGAGTGATCAACCCTACAGGCTTCAGAAGTTCGGGTTGCGGTTGTTTTATGAGTCCGATATTCGGCAACCAAACTTAGAGGTAGCAAAAGAAAGGTTGAAACAAGTCATAACCCAGGCTGCGGAGAAAGATCTTTCTGAATTGTGCTCGGACCTACCTCAAATTGATAATGCATTATCCATATCGGAATCTGAAGTTCAACCATCATGGTTTCAGTTTTTCAATAAAGAGCTTGTGCATACAGTGTCTTTTTCAGACCATGAAGCTTTTGATCATCCTGTGGCATGTCTCGTTGTAGTTTCTTCAAAGGATGATCAGCCCATCAACAGATTTGTTGACCTCTTTAACTCGACCAAGTTGCCCTCCCTTCTTACTAATGGGGCAATGGACCCAAGGATTTTGAAGCATTATTTACTAGTGCATGATAATCAAGATGGACCTTTAGAGAAGGCAACTAAAATTTTAACTGAAATGAGGAGCACTTTTGGCTCTGACTGTCAATTACTATGCATTAATTCTTCTCTAGATGGTGTGGTTGAACATCAAGATTATCCCTGGGTGCTTTATAAATTTGAAGATTTACCTACTCAACCGCTTCGCTGCTTCCTTAATATTAACGATTTTAATGAGATAAAGGATCTCATGCAAGATTTATCAACTAAACACATTATTCCCTACATGGAGCAAAAAATACGTGCGCTCAATCAGCAGGTTGCTGCAACAAGGAAAGGTTTCAGGAATCAAATAAAAAATCTATGGTGGAGAAAAGGAAAAGATGATGTGGTGGATTCTCCCAGTGGCCCTATGTATACCTTTAACTCCATTGAATCCCAAATTAGAGTTTTGGGTGATTATGCCTTCATGCTATGTGATTATGAACTTGCATTGTCAAATTATCGCCTAATATCCACAGATTACAAGCTTGACAAAGCCTGGAAACGCTATGCTGGCGTACAGGAAATGATGGGGCTTGCATATTTCATGTTAGACCAATCAAGAAAGGATGCAGAGTATTGCATGGAAAACGCATTTACTACCTATTTAAAAGTTGCACCATCTAGCCAGCAAAATGCAACTAGGTGTGGTCTTTGGTGGGTGGAGATGTTGAAGACAAGATATCAGTATAAAGAGGCTGCAACTGTTTACTTCCGTGTTTGTACTGAGGAGCCGCTACATTCTGCTGTAATGCTTGAGCAAGCTTCTTATTGTTACTTGCTGTCTAGACCGCCTATGTTGCACAAATATGGATTTCATCTTGTTCTTTCTGGTGACCGTTACAAGAAATGTGATCAGGTCAAACATGCAATTCGCACATATAGAGGTGCCATGTCTGTGTATACAGGAACTACGTGGAGTCATATCAAAGACCATGTTCATTTCCATATTGGACAGTGGTATGCTCTTCTTGGGCTATATGATTTGGCTGTAAATCATGTGTTGGAGGTCCTGGCCTGTAGTCATCAGTCCAAGAAAACGCAGGAGTTATTCCTGAGAGACTTCCTTCAAATTGTTCAGAAAGCAGGGAAAGCAGTTGAAGTATCAAAGCTTCAATTGCCAGAGATCAATATTTCATCTGTTAGGGTAATTTTTGAGGATCATCGGACTTATGCATCATCTGCAGCTGCAAATGTCAAGGAAAAAATATGGGTCTCCTTAGAGGAGGAAATGATTCCAAATTTGTCTACCACTAGAACTAATTGGTTGGAGTTACAATCAAAGCTTGTCCCAAAGAAGTACAAAGATTCAAATGTTTGTGTTGCAGGAGAAGCTGTCAGGATTGATATTGAATTCAAAAACCCATTGCAAATCCCCCTCCTCCTGTCTAGTGTTTCCCTAATATGCGAGCTTTCTGCAGGCTCTGATGAAATGAAATCTGATGCAAGTAGCTCGTTGACGGAGATTCAGGATGGGGAATCTACTAATTTAATTCATAGGGATGTGAACCTTGAGAGTTCTTTATTTTCTTTGTCTGATGTTGATTTTTCATTAGCAGGAGGTGAAAAAATTGTGGTGCAACTAACGGTTACTCCTAGAATAGAAGGCATTTTACAGATTGTTGGTGTAAAGTGGAAGTTGTCAGGTTCTGTTGTTGGCTTCCATAAATTTGACACTAACCCCATGAAGAAGATAAGCAGAAAACGAATACAGAAGGCTAAGCATCCTCATAGCGATAATTTAAAGTTTGTAGTTGTTAAGAGTGTACCCAAGCTTGAGGGTGTCATTCATCCACCACCTAAAAGGGCATATGCGGGAGATTTACGGCATCTTGTGTTGGAGTTAAAGAACAAATCTGAATTTGCTGTGAAGAATCTGAAAATAAAGATTAGTCATCCTAGATTCTTGAATCTTGGAAAGCAAGAAAGTTTGAACACCGAGTTTCCTGCTTGCTTAGAAAAGACAAATTCTGATCGGTCTGCTGAACATGCTAACCCCAATGACATTTCTCAGGCCCTGTTTCTATTTCCAGAGGACACAATTATTCAAGGGGAAACACCATTATTGTGGCCTCTTTGGTTTCGGGCAGCTGTTCCTGGGAACATTTCCTTGTACATAACTATATATTATGAGATGGGCGATACTTCAAGCACCATGAGATATCGCACTTTACGCATGCATTACAATTTGCAGGTATTGCCGTCATTGGATGTGTCATTCCTTATTAGTCCCTGTCCATCAAGATTGCAAGAGTTTCTTGTGCGCATGGATGTTGTCAACAAGACAAGCTCTGAAAGTTTCGAAATTCAACAGTTGTCATCTGTTGGGAACCAATGGGAAATCTCATTACTTCAACCTGTCGATGACATCATTCCTTCACAATCTTTGACCGCTCATCAAGCATTGTCATGTTTCTTCATGCTCAAGAATCATGGCAAGTCATCAACTTCTGAAGATGAGAAATCTTCCCATTCCCGACTTCGGAGAACTGATTTGAGATTCAGTAATGGACCACTTTTTGATATAGCTAGCTCTCCCCTGGCAGATTTCCACCATTCTGAAAGACTGCACCAGGAAATTTTACATAAGGGAGACACTAATCCAGTTGATTTCATATTGATTTCCCGGCCTTTGAAGAATGACATTAATCCTGAAGTGTCCGAGCCACCGCATCTTTATTCTCATCATGCATGTCACTGCAGTACTGCAAGCCCAAGTCCTATATCATGGCTAGTGGACGGACCTCGAACCTTGTATCATAACTTCTCTGCTTCCTTTTGTGAAATAAATCTTAGCATGACAATCTACAACTCATCAGATGTCGTTTCATCTGTACGCATAAACACCTCAGACTCCAGTACTAGTGATCATTCAGGCGATGCAACTCCAGTTCAACCTGCCATGTCTTCTGGCAACCAAGATGGATGGCATGACCTCTCACTAGCGACTGACATCAAAGTGACCTCCGATGCCCTTGGATCCCAGGTCAGTAAGTCAATTCCCGTGGAGAGCGTCTCCCCATTTATTTGGTCCGGGTCAAGCTCAACTAGAGTCCAACTTGATCCTATGTCAAGAACTGAAATTCCTCTTCAAGTTTGTGTATTCTCTCCCGGGACACATGATCTGTCGAACTATGTTTTGCATTGGAACCTTTTACTTTCTAACGATCAAGAAAATTGGGATGGAAGGTCTTCCGGGACATGCCAGGGATATCCGTATTACCTTACCGTACTCCAGTCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPPC-Trs85 TRAPPC9-Trs120

Protein Analysis

1286

Amino Acids

145.61

Weight (kDa)

6.12

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC-Trs85 PF12739 16 - 431 2.3e-85 ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Ig_TPPC8_1st PF24545 604 - 758 1.3e-14 TPPC8 first Ig-like domain
Ig_TPPC8_2nd PF24544 810 - 928 1.1e-07 TPPC8 second Ig-like domain
Ig_TPPC8_C PF24542 1189 - 1248 2.4e-06 TPPC8 C-terminal Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16280 AT5G16280
fragaria_vesca FvH4_6g20450 FvH4_6g20450 FvH4_6g20450 FvH4_6g20450
malus_domestica MD12G1041600.v1.1 MD14G1040700.v1.1
prunus_persica Prupe.7G093600_v2.0.a1
pyrus_communis pycom12g03630 pycom14g03450
rosa_chinensis RchiOBHm_Chr3g0476301
rosa_laevigata RLG00000023780
rosa_multiflora Rmu_sc0000114.1_g000012
rosa_roxburghii Rroxscaffold_6G00405410
rosa_rugosa Rorug03G0153400
rosa_samantha Rh3AG204600 Rh3BG236000 Rh3CG230700 Rh3DG230500
rosa_wichuraiana Rw3G018590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
Acc16I TGCGCA 1 cut(s) 2876
Acc36I ACCTGC 3 cut(s) 877, 2794, 3517
AccB1I GGYRCC 1 cut(s) 1499
AccBSI CCGCTC 1 cut(s) 3005
AccI GTMKAC 3 cut(s) 986, 1514, 1859
AciI CCGC 9 cut(s) 172, 217, 299, 770, 1346, 1403, 2426, 3003, 3281
AclWI GGATC 7 cut(s) 2, 15, 822, 1779, 3585, 3598, 3668
AcuI CTGAAG 4 cut(s) 185, 390, 3084, 3285
AfiI CCNNNNNNNGG 3 cut(s) 240, 298, 3093
AhlI ACTAGT 2 cut(s) 592, 3473
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 9 cut(s) 291, 731, 1109, 1623, 2707, 3150, 3183, 3594, 3816
AloI GAACNNNNNNTCC 2 cut(s) 3679, 3711
Alw21I GWGCWC 2 cut(s) 326, 659
Alw26I GTCTC 5 cut(s) 470, 1665, 1831, 3198, 3631
AlwI GGATC 7 cut(s) 2, 15, 822, 1779, 3585, 3598, 3668
AlwNI CAGNNNCTG 3 cut(s) 2036, 2261, 2629
Ama87I CYCGRG 1 cut(s) 3722
AoxI GGCC 6 cut(s) 109, 976, 1626, 2625, 2681, 3239
ApeKI GCWGC 7 cut(s) 296, 777, 893, 1310, 1796, 1799, 2699
AseI ATTAAT 2 cut(s) 690, 3258
Asp700I GAANNNNTTC 3 cut(s) 908, 1845, 3802
AspLEI GCGC 2 cut(s) 877, 2877
AsuC2I CCSGG 5 cut(s) 3238, 3646, 3723, 3724, 3808
AsuHPI GGTGA 4 cut(s) 106, 1037, 1457, 2186
AsuII TTCGAA 1 cut(s) 2913
AsuNHI GCTAGC 1 cut(s) 3140
AvaI CYCGRG 1 cut(s) 3722
AvaII GGWCC 8 cut(s) 67, 328, 565, 614, 1621, 3122, 3353, 3642
BamHI GGATCC 2 cut(s) 7, 3590
BanI GGYRCC 1 cut(s) 1499
BarI GAAGNNNNNNTAC 6 cut(s) 1707, 1739, 2208, 2240, 3359, 3391
BbsI GAAGAC 4 cut(s) 139, 1292, 3510, 3795
Bbv12I GWGCWC 2 cut(s) 326, 659
BbvI GCAGC 7 cut(s) 283, 764, 880, 1297, 1786, 1808, 2711
BceAI ACGGC 3 cut(s) 124, 2453, 2797
BcgI CGANNNNNNTGC 8 cut(s) 517, 551, 2009, 2043, 2046, 2080, 2540, 2574
BciT130I CCWGG 9 cut(s) 293, 733, 1111, 1625, 2709, 3152, 3185, 3596, 3818
BclI TGATCA 5 cut(s) 184, 448, 490, 1465, 3478
BcnI CCSGG 5 cut(s) 3238, 3646, 3723, 3724, 3808
BcoDI GTCTC 5 cut(s) 470, 1665, 1831, 3198, 3631
BcuI ACTAGT 2 cut(s) 592, 3473
BfmI CTRYAG 5 cut(s) 193, 1630, 1794, 2028, 3310
BfuAI ACCTGC 3 cut(s) 877, 2794, 3517
BglII AGATCT 1 cut(s) 307
BisI GCNGC 8 cut(s) 297, 778, 894, 1311, 1346, 1797, 1800, 2700
BlpI GCTNAGC 1 cut(s) 2331
BlsI GCNGC 8 cut(s) 298, 779, 895, 1312, 1347, 1798, 1801, 2701
BmcAI AGTACT 2 cut(s) 3316, 3472
Bme18I GGWCC 8 cut(s) 67, 328, 565, 614, 1621, 3122, 3353, 3642
BmeT110I CYCGRG 1 cut(s) 3722
BmgBI CACGTC 1 cut(s) 163
BmiI GGNNCC 7 cut(s) 9, 567, 1344, 1501, 2942, 3592, 3761
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 8 cut(s) 1171, 1796, 2044, 2344, 2449, 3292, 3481, 3571
BmtI GCTAGC 1 cut(s) 3144
BmuI ACTGGG 1 cut(s) 966
BpiI GAAGAC 4 cut(s) 139, 1292, 3510, 3795
BplI GAGNNNNNCTC 2 cut(s) 3611, 3643
BpmI CTGGAG 3 cut(s) 3451, 3483, 3830
Bpu1102I GCTNAGC 1 cut(s) 2331
Bpu14I TTCGAA 1 cut(s) 2913
BpuEI CTTGAG 6 cut(s) 86, 116, 1388, 2144, 2408, 3020
BpuMI CCSGG 5 cut(s) 3238, 3646, 3723, 3724, 3808
BsaAI YACGTR 2 cut(s) 872, 1527
BsaI GGTCTC 1 cut(s) 1831
BsaXI ACNNNNNCTCC 4 cut(s) 1932, 1962, 2188, 2218
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 298, 3093
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 5 cut(s) 972, 3215, 3468, 3500, 3847
Bse3DI GCAATG 2 cut(s) 567, 1976
BseBI CCWGG 9 cut(s) 293, 733, 1111, 1625, 2709, 3152, 3185, 3596, 3818
BseLI CCNNNNNNNGG 3 cut(s) 240, 298, 3093
BseMI GCAATG 2 cut(s) 567, 1976
BseMII CTCAG 4 cut(s) 1329, 1658, 2636, 3474
BseNI ACTGG 5 cut(s) 972, 3215, 3468, 3500, 3847
BseRI GAGGAG 5 cut(s) 59, 667, 1355, 1850, 1982
BseXI GCAGC 7 cut(s) 283, 764, 880, 1297, 1786, 1808, 2711
BsgI GTGCAG 1 cut(s) 3164
BshFI GGCC 6 cut(s) 111, 978, 1628, 2627, 2683, 3241
BshNI GGYRCC 1 cut(s) 1499
BsiHKAI GWGCWC 2 cut(s) 326, 659
BsiHKCI CYCGRG 1 cut(s) 3722
BsiSI CCGG 6 cut(s) 11, 167, 3238, 3645, 3723, 3807
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 4 cut(s) 1886, 2826, 3739, 3823
BslI CCNNNNNNNGG 3 cut(s) 240, 298, 3093
BsmAI GTCTC 5 cut(s) 470, 1665, 1831, 3198, 3631
BsmBI CGTCTC 1 cut(s) 3631
BsmFI GGGAC 4 cut(s) 1886, 2826, 3739, 3823
BsnI GGCC 6 cut(s) 111, 978, 1628, 2627, 2683, 3241
Bso31I GGTCTC 1 cut(s) 1831
BsoBI CYCGRG 1 cut(s) 3722
Bsp119I TTCGAA 1 cut(s) 2913
Bsp1286I GDGCHC 2 cut(s) 326, 659
Bsp1407I TGTACA 1 cut(s) 2724
Bsp1720I GCTNAGC 1 cut(s) 2331
BspACI CCGC 9 cut(s) 172, 217, 299, 770, 1346, 1403, 2426, 3003, 3281
BspANI GGCC 6 cut(s) 111, 978, 1628, 2627, 2683, 3241
BspCNI CTCAG 4 cut(s) 1330, 1659, 2635, 3473
BspLI GGNNCC 7 cut(s) 9, 567, 1344, 1501, 2942, 3592, 3761
BspMAI CTGCAG 3 cut(s) 1798, 2032, 3314
BspMI ACCTGC 3 cut(s) 877, 2794, 3517
BspOI GCTAGC 1 cut(s) 3144
BspPI GGATC 7 cut(s) 2, 15, 822, 1779, 3585, 3598, 3668
BspQI GCTCTTC 1 cut(s) 1584
BspT104I TTCGAA 1 cut(s) 2913
BspT107I GGYRCC 1 cut(s) 1499
BspTNI GGTCTC 1 cut(s) 1831
BsrBI CCGCTC 1 cut(s) 3005
BsrDI GCAATG 2 cut(s) 567, 1976
BsrFI RCCGGY 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 2724
BsrI ACTGG 5 cut(s) 972, 3215, 3468, 3500, 3847
BssAI RCCGGY 1 cut(s) 166
BssNAI GTATAC 2 cut(s) 987, 1515
BssT1I CCWWGG 2 cut(s) 569, 3586
Bst1107I GTATAC 2 cut(s) 987, 1515
Bst2UI CCWGG 9 cut(s) 293, 733, 1111, 1625, 2709, 3152, 3185, 3596, 3818
Bst4CI ACNGT 8 cut(s) 421, 674, 1318, 1451, 1569, 2200, 2925, 3841
Bst6I CTCTTC 2 cut(s) 1584, 3705
BstAPI GCANNNNNTGC 2 cut(s) 1793, 2809
BstAUI TGTACA 1 cut(s) 2724
BstBAI YACGTR 2 cut(s) 872, 1527
BstBI TTCGAA 1 cut(s) 2913
BstDEI CTNAG 9 cut(s) 253, 1338, 1667, 1831, 2331, 2566, 2622, 3404, 3460
BstDSI CCRYGG 1 cut(s) 3615
BstEII GGTNACC 1 cut(s) 1445
BstHHI GCGC 2 cut(s) 877, 2877
BstMAI GTCTC 5 cut(s) 470, 1665, 1831, 3198, 3631
BstNI CCWGG 9 cut(s) 293, 733, 1111, 1625, 2709, 3152, 3185, 3596, 3818
BstNSI RCATGY 6 cut(s) 465, 1481, 2603, 2791, 3306, 3816
BstPI GGTNACC 1 cut(s) 1445
BstSFI CTRYAG 5 cut(s) 193, 1630, 1794, 2028, 3310
BstV1I GCAGC 7 cut(s) 283, 764, 880, 1297, 1786, 1808, 2711
BstV2I GAAGAC 4 cut(s) 139, 1292, 3510, 3795
BstX2I RGATCY 4 cut(s) 7, 307, 814, 3590
BstXI CCANNNNNNTGG 2 cut(s) 2679, 3639
BstYI RGATCY 4 cut(s) 7, 307, 814, 3590
BstZ17I GTATAC 2 cut(s) 987, 1515
BsuRI GGCC 6 cut(s) 111, 978, 1628, 2627, 2683, 3241
BtgI CCRYGG 1 cut(s) 3615
BtgZI GCGATG 1 cut(s) 3504
BtrI CACGTC 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 3307
BtsIMutI CAGTG 4 cut(s) 426, 979, 1574, 3307
BveI ACCTGC 3 cut(s) 877, 2794, 3517
CaiI CAGNNNCTG 3 cut(s) 2036, 2261, 2629
CfoI GCGC 2 cut(s) 877, 2877
Cfr10I RCCGGY 1 cut(s) 166
Cfr9I CCCGGG 1 cut(s) 3722
CseI GACGC 2 cut(s) 133, 3613
CspCI CAANNNNNGTGG 2 cut(s) 3597, 3632
DdeI CTNAG 9 cut(s) 253, 1338, 1667, 1831, 2331, 2566, 2622, 3404, 3460
DraI TTTAAA 2 cut(s) 1221, 2355
Eam1104I CTCTTC 2 cut(s) 1584, 3705
EarI CTCTTC 2 cut(s) 1584, 3705
Eco130I CCWWGG 2 cut(s) 569, 3586
Eco31I GGTCTC 1 cut(s) 1831
Eco32I GATATC 3 cut(s) 1295, 2774, 3824
Eco47I GGWCC 8 cut(s) 67, 328, 565, 614, 1621, 3122, 3353, 3642
Eco57I CTGAAG 4 cut(s) 185, 390, 3084, 3285
Eco88I CYCGRG 1 cut(s) 3722
Eco91I GGTNACC 1 cut(s) 1445
EcoO109I RGGNCCY 2 cut(s) 1621, 2626
EcoO65I GGTNACC 1 cut(s) 1445
EcoRI GAATTC 1 cut(s) 1964
EcoRII CCWGG 9 cut(s) 291, 731, 1109, 1623, 2707, 3150, 3183, 3594, 3816
EcoRV GATATC 3 cut(s) 1295, 2774, 3824
EcoT14I CCWWGG 2 cut(s) 569, 3586
EcoT22I ATGCAT 5 cut(s) 352, 689, 1789, 2793, 3304
ErhI CCWWGG 2 cut(s) 569, 3586
Esp3I CGTCTC 1 cut(s) 3631
FalI AAGNNNNNCTT 2 cut(s) 2853, 2885
FaqI GGGAC 4 cut(s) 1886, 2826, 3739, 3823
FauI CCCGC 1 cut(s) 2419
FauNDI CATATG 1 cut(s) 2422
FbaI TGATCA 5 cut(s) 184, 448, 490, 1465, 3478
FblI GTMKAC 3 cut(s) 986, 1514, 1859
Fnu4HI GCNGC 8 cut(s) 297, 778, 894, 1311, 1346, 1797, 1800, 2700
Fsp4HI GCNGC 8 cut(s) 297, 778, 894, 1311, 1346, 1797, 1800, 2700
FspAI RTGCGCAY 1 cut(s) 2876
FspI TGCGCA 1 cut(s) 2876
GlaI GCGC 2 cut(s) 876, 2876
GluI GCNGC 8 cut(s) 297, 778, 894, 1311, 1346, 1797, 1800, 2700
GsuI CTGGAG 3 cut(s) 3451, 3483, 3830
HaeIII GGCC 6 cut(s) 111, 978, 1628, 2627, 2683, 3241
HapII CCGG 6 cut(s) 11, 167, 3238, 3645, 3723, 3807
HgaI GACGC 2 cut(s) 133, 3613
HhaI GCGC 2 cut(s) 877, 2877
Hin6I GCGC 2 cut(s) 875, 2875
HinP1I GCGC 2 cut(s) 875, 2875
HincII GTYRAC 3 cut(s) 514, 2070, 2890
HindII GTYRAC 3 cut(s) 514, 2070, 2890
HindIII AAGCTT 7 cut(s) 93, 441, 1097, 1374, 1721, 1895, 2384
HpaII CCGG 6 cut(s) 11, 167, 3238, 3645, 3723, 3807
HphI GGTGA 4 cut(s) 106, 1037, 1457, 2186
Hpy99I CGWCG 2 cut(s) 89, 164
HpyCH4III ACNGT 8 cut(s) 421, 674, 1318, 1451, 1569, 2200, 2925, 3841
HpyCH4IV ACGT 3 cut(s) 162, 871, 1526
HpyF3I CTNAG 9 cut(s) 253, 1338, 1667, 1831, 2331, 2566, 2622, 3404, 3460
HpySE526I ACGT 3 cut(s) 162, 871, 1526
HspAI GCGC 2 cut(s) 875, 2875
Ksp22I TGATCA 5 cut(s) 184, 448, 490, 1465, 3478
LguI GCTCTTC 1 cut(s) 1584
LmnI GCTCC 3 cut(s) 654, 859, 1342
Lsp1109I GCAGC 7 cut(s) 283, 764, 880, 1297, 1786, 1808, 2711
LweI GCATC 8 cut(s) 1171, 1796, 2044, 2344, 2449, 3292, 3481, 3571
MaeII ACGT 3 cut(s) 162, 871, 1526
MaeIII GTNAC 7 cut(s) 1385, 1445, 1451, 1884, 2200, 3305, 3571
MbiI CCGCTC 1 cut(s) 3005
MfeI CAATTG 1 cut(s) 1727
MflI RGATCY 4 cut(s) 7, 307, 814, 3590
MhlI GDGCHC 2 cut(s) 326, 659
MlyI GAGTC 4 cut(s) 239, 1540, 3458, 3672
MmeI TCCRAC 4 cut(s) 1596, 1860, 2430, 3691
Mph1103I ATGCAT 5 cut(s) 352, 689, 1789, 2793, 3304
MroXI GAANNNNTTC 3 cut(s) 908, 1845, 3802
MslI CAYNNNNRTG 7 cut(s) 161, 1419, 1508, 2112, 2677, 3019, 3569
MspA1I CMGCKG 2 cut(s) 1799, 2702
MspI CCGG 6 cut(s) 11, 167, 3238, 3645, 3723, 3807
MunI CAATTG 1 cut(s) 1727
MvaI CCWGG 9 cut(s) 293, 733, 1111, 1625, 2709, 3152, 3185, 3596, 3818
NciI CCSGG 5 cut(s) 3238, 3646, 3723, 3724, 3808
NdeI CATATG 1 cut(s) 2422
NheI GCTAGC 1 cut(s) 3140
NlaIV GGNNCC 7 cut(s) 9, 567, 1344, 1501, 2942, 3592, 3761
NmuCI GTSAC 3 cut(s) 1445, 3305, 3571
NsbI TGCGCA 1 cut(s) 2876
NsiI ATGCAT 5 cut(s) 352, 689, 1789, 2793, 3304
NspI RCATGY 6 cut(s) 465, 1481, 2603, 2791, 3306, 3816
NspV TTCGAA 1 cut(s) 2913
PaeI GCATGC 1 cut(s) 2791
PasI CCCWGGG 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 1584
PdmI GAANNNNTTC 3 cut(s) 908, 1845, 3802
Pfl23II CGTACG 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 3806
PkrI GCNGC 8 cut(s) 298, 779, 895, 1312, 1347, 1798, 1801, 2701
PleI GAGTC 4 cut(s) 238, 1539, 3458, 3671
PpsI GAGTC 4 cut(s) 238, 1539, 3458, 3671
Ppu21I YACGTR 2 cut(s) 872, 1527
PpuMI RGGWCCY 1 cut(s) 1621
PshBI ATTAAT 2 cut(s) 690, 3258
PsiI TTATAA 1 cut(s) 744
Psp5II RGGWCCY 1 cut(s) 1621
Psp6I CCWGG 9 cut(s) 291, 731, 1109, 1623, 2707, 3150, 3183, 3594, 3816
PspEI GGTNACC 1 cut(s) 1445
PspGI CCWGG 9 cut(s) 291, 731, 1109, 1623, 2707, 3150, 3183, 3594, 3816
PspLI CGTACG 1 cut(s) 74
PspN4I GGNNCC 7 cut(s) 9, 567, 1344, 1501, 2942, 3592, 3761
PspPPI RGGWCCY 1 cut(s) 1621
PstI CTGCAG 3 cut(s) 1798, 2032, 3314
PstNI CAGNNNCTG 3 cut(s) 2036, 2261, 2629
PsuI RGATCY 4 cut(s) 7, 307, 814, 3590
PvuII CAGCTG 2 cut(s) 1799, 2702
RseI CAYNNNNRTG 7 cut(s) 161, 1419, 1508, 2112, 2677, 3019, 3569
SapI GCTCTTC 1 cut(s) 1584
SatI GCNGC 8 cut(s) 297, 778, 894, 1311, 1346, 1797, 1800, 2700
ScaI AGTACT 2 cut(s) 3316, 3472
SchI GAGTC 4 cut(s) 239, 1540, 3458, 3672
SduI GDGCHC 2 cut(s) 326, 659
SfaNI GCATC 8 cut(s) 1171, 1796, 2044, 2344, 2449, 3292, 3481, 3571
SfcI CTRYAG 5 cut(s) 193, 1630, 1794, 2028, 3310
SfuI TTCGAA 1 cut(s) 2913
SinI GGWCC 8 cut(s) 67, 328, 565, 614, 1621, 3122, 3353, 3642
SmaI CCCGGG 1 cut(s) 3724
SmiMI CAYNNNNRTG 7 cut(s) 161, 1419, 1508, 2112, 2677, 3019, 3569
SmlI CTYRAG 6 cut(s) 101, 131, 1367, 2123, 2387, 3035
SmoI CTYRAG 6 cut(s) 101, 131, 1367, 2123, 2387, 3035
SpeI ACTAGT 2 cut(s) 592, 3473
SphI GCATGC 1 cut(s) 2791
SsiI CCGC 9 cut(s) 172, 217, 299, 770, 1346, 1403, 2426, 3003, 3281
SspI AATATT 2 cut(s) 790, 1744
StyI CCWWGG 2 cut(s) 569, 3586
TaaI ACNGT 8 cut(s) 421, 674, 1318, 1451, 1569, 2200, 2925, 3841
TaiI ACGT 3 cut(s) 165, 874, 1529
TaqI TCGA 7 cut(s) 87, 159, 527, 2913, 2973, 3358, 3740
TaqII GACCGA 1 cut(s) 2577
TatI WGTACW 5 cut(s) 1334, 1911, 2724, 3314, 3470
TauI GCSGC 1 cut(s) 1348
TscAI CASTG 4 cut(s) 426, 979, 1574, 3314
TseFI GTSAC 3 cut(s) 1445, 3305, 3571
TseI GCWGC 7 cut(s) 296, 777, 893, 1310, 1796, 1799, 2699
Tsp45I GTSAC 3 cut(s) 1445, 3305, 3571
TspGWI ACGGA 5 cut(s) 62, 1316, 2087, 3366, 3816
TspMI CCCGGG 1 cut(s) 3722
TspRI CASTG 4 cut(s) 426, 979, 1574, 3314
VpaK11BI GGWCC 8 cut(s) 67, 328, 565, 614, 1621, 3122, 3353, 3642
VspI ATTAAT 2 cut(s) 690, 3258
XbaI TCTAGA 2 cut(s) 700, 1397
XceI RCATGY 6 cut(s) 465, 1481, 2603, 2791, 3306, 3816
XmaI CCCGGG 1 cut(s) 3722
XmiI GTMKAC 3 cut(s) 986, 1514, 1859
XmnI GAANNNNTTC 3 cut(s) 908, 1845, 3802
ZrmI AGTACT 2 cut(s) 3316, 3472
Zsp2I ATGCAT 5 cut(s) 352, 689, 1789, 2793, 3304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.