AT5G20620

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017038
Physical Location & Seq
Reverse (-)
6973053 .. 6975009
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20620.2

Sequence Viewer

Length: 1149 bp
ATGCAAATTTTCGTGAAAACACTCACTGGTAAGACCATCACTCTCGAGGTTGAGAGCTCTGACACCATCGACAATGTTAAGGCAAAGATCCAGGACAAGGAAGGCATTCCTCCTGACCAGCAAAGATTGATCTTCGCTGGTAAGCAGCTAGAAGATGGCCGCACCTTGGCTGATTACAACATCCAGAAAGAATCAACACTCCACTTGGTTCTCAGGTTAAGAGGTGGTATGCAGATCTTTGTCAAGACTCTGACTGGCAAAACCATTACCTTGGAGGTTGAAAGCTCTGACACTATTGACAACGTCAAGGCAAAGATCCAGGACAAGGAAGGAATCCCTCCGGACCAGCAAAGACTTATTTTCGCTGGTAAGCAGCTTGAAGATGGAAGGACTCTTGCTGACTACAATATCCAAAAGGAGTCTACTCTTCACCTTGTCCTTCGTCTCCGTGGTGGTATGCAAATCTTTGTGAAGACTCTGACGGGTAAAACAATCACCCTTGAGGTTGAGAGCTCAGACACCATTGACAATGTCAAAGCTAAGATCCAAGATAAAGAGGGAATCCCACCGGACCAGCAGAGGCTTATCTTTGCCGGTAAGCAGCTAGAGGATGGTCGCACTCTTGCTGATTACAACATCCAAAAAGAGTCAACCCTTCATTTGGTGCTTCGTCTGAGAGGTGGTATGCAGATTTTTGTGAAGACCTTAACTGGCAAGACTATCACTTTGGAGGTTGAGAGCTCAGACACAATCGATAATGTCAAGGCAAAGATTCAAGACAAGGAAGGGATTCCACCAGATCAGCAGAGACTCATATTTGCTGGAAAACAGCTTGAAGATGGACGCACACTCGCTGATTACAACATCCAAAAGGAGTCTACCCTCCATCTTGTGCTTCGTCTCCGTGGTGGTATGCAGATATTTGTGAAGACCCTTACCGGAAAAACCATTACTCTTGAGGTTGAGAGCTCAGACACAATTGATAATGTCAAGGCTAAGATTCAGGACAAGGAAGGGATCCCACCGGACCAACAGAGACTCATCTTCGCTGGGAAGCAGCTTGAGGATGGTCGGACTCTTGCAGATTACAACATCCAGAAGGAGTCGACTCTTCACTTGGTTCTTCGTCTTCGTGGTGGAAGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

382

Amino Acids

42.79

Weight (kDa)

7.0

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 77 - 147 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 153 - 223 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 229 - 299 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 7.4e-32 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017038)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20620
fragaria_vesca FvH4_3g21853
prunus_persica Prupe.4G025900_v2.0.a1 Prupe.6G238900_v2.0.a1
pyrus_communis pycom12g11970
rosa_chinensis RchiOBHm_Chr3g0469331
rosa_laevigata RLG00000024359 RLG00000031189 RLG00000033799
rosa_samantha Rh3AG149900 Rh3BG173500 Rh7AG256200 Rh7CG272600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 422, 878, 1106
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 1 cut(s) 160
AclWI GGATC 5 cut(s) 82, 310, 538, 1012, 1025
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 6
AfiI CCNNNNNNNGG 4 cut(s) 97, 166, 325, 661
AgsI TTSAA 4 cut(s) 281, 380, 776, 836
AjnI CCWGG 2 cut(s) 90, 318
Alw21I GWGCWC 4 cut(s) 59, 515, 743, 971
Alw26I GTCTC 4 cut(s) 449, 802, 905, 1030
AlwI GGATC 5 cut(s) 82, 310, 538, 1012, 1025
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 1 cut(s) 157
ApeKI GCWGC 4 cut(s) 145, 373, 601, 1057
ApoI RAATTY 1 cut(s) 6
ArsI GACNNNNNNTTYG 2 cut(s) 709, 741
Asp700I GAANNNNTTC 2 cut(s) 105, 789
AspS9I GGNCC 3 cut(s) 343, 571, 1027
AsuHPI GGTGA 2 cut(s) 422, 487
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 3 cut(s) 343, 571, 1027
BamHI GGATCC 1 cut(s) 1017
BanII GRGCYC 4 cut(s) 59, 515, 743, 971
BbsI GAAGAC 4 cut(s) 479, 707, 935, 1121
Bbv12I GWGCWC 4 cut(s) 59, 515, 743, 971
BbvI GCAGC 4 cut(s) 157, 385, 613, 1069
BccI CCATC 8 cut(s) 44, 74, 149, 377, 605, 833, 894, 1061
BciT130I CCWGG 2 cut(s) 92, 320
BcoDI GTCTC 4 cut(s) 449, 802, 905, 1030
BfaI CTAG 2 cut(s) 149, 605
BglII AGATCT 1 cut(s) 234
BisI GCNGC 5 cut(s) 146, 160, 374, 602, 1058
BlsI GCNGC 5 cut(s) 147, 161, 375, 603, 1059
Bme1390I CCNGG 2 cut(s) 92, 320
Bme18I GGWCC 3 cut(s) 343, 571, 1027
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 3 cut(s) 343, 571, 1027
BmiI GGNNCC 1 cut(s) 1019
BmrFI CCNGG 2 cut(s) 92, 320
BpiI GAAGAC 4 cut(s) 479, 707, 935, 1121
BpuEI CTTGAG 3 cut(s) 521, 977, 1082
Bsa29I ATCGAT 1 cut(s) 753
BsaJI CCNNGG 4 cut(s) 165, 270, 448, 904
BsaWI WCCGGW 4 cut(s) 340, 568, 938, 1024
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 166, 325, 661
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 3 cut(s) 31, 259, 715
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 2 cut(s) 92, 320
BseCI ATCGAT 1 cut(s) 753
BseDI CCNNGG 4 cut(s) 165, 270, 448, 904
BseGI GGATG 6 cut(s) 180, 616, 636, 864, 1072, 1092
BseLI CCNNNNNNNGG 4 cut(s) 97, 166, 325, 661
BseMII CTCAG 5 cut(s) 226, 528, 665, 756, 984
BseNI ACTGG 3 cut(s) 31, 259, 715
BseXI GCAGC 4 cut(s) 157, 385, 613, 1069
BseYI CCCAGC 1 cut(s) 1049
BshFI GGCC 1 cut(s) 159
BshVI ATCGAT 1 cut(s) 753
BsiHKAI GWGCWC 4 cut(s) 59, 515, 743, 971
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 5 cut(s) 341, 569, 594, 939, 1025
BslI CCNNNNNNNGG 4 cut(s) 97, 166, 325, 661
BsmAI GTCTC 4 cut(s) 449, 802, 905, 1030
BsmBI CGTCTC 2 cut(s) 449, 905
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 4 cut(s) 59, 515, 743, 971
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
BspACI CCGC 1 cut(s) 160
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 5 cut(s) 225, 527, 666, 755, 983
BspDI ATCGAT 1 cut(s) 753
BspEI TCCGGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 1019
BspPI GGATC 5 cut(s) 82, 310, 538, 1012, 1025
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 3 cut(s) 31, 259, 715
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 4 cut(s) 165, 270, 448, 904
BssMI GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
BssT1I CCWWGG 2 cut(s) 165, 270
Bst2UI CCWGG 2 cut(s) 92, 320
Bst6I CTCTTC 2 cut(s) 432, 1116
BstDEI CTNAG 7 cut(s) 212, 514, 540, 674, 742, 970, 996
BstDSI CCRYGG 2 cut(s) 448, 904
BstF5I GGATG 6 cut(s) 180, 616, 636, 864, 1072, 1092
BstKTI GATC 7 cut(s) 90, 132, 237, 318, 546, 802, 1020
BstMAI GTCTC 4 cut(s) 449, 802, 905, 1030
BstMBI GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
BstNI CCWGG 2 cut(s) 92, 320
BstSCI CCNGG 2 cut(s) 90, 318
BstV1I GCAGC 4 cut(s) 157, 385, 613, 1069
BstV2I GAAGAC 4 cut(s) 479, 707, 935, 1121
BstX2I RGATCY 5 cut(s) 87, 234, 315, 543, 1017
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 5 cut(s) 87, 234, 315, 543, 1017
Bsu15I ATCGAT 1 cut(s) 753
BsuRI GGCC 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 753
BtgI CCRYGG 2 cut(s) 448, 904
BtsCI GGATG 6 cut(s) 180, 616, 636, 864, 1072, 1092
BtsIMutI CAGTG 1 cut(s) 24
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 3 cut(s) 343, 571, 1027
ClaI ATCGAT 1 cut(s) 753
CseI GACGC 1 cut(s) 852
DdeI CTNAG 7 cut(s) 212, 514, 540, 674, 742, 970, 996
DpnI GATC 7 cut(s) 89, 131, 236, 317, 545, 801, 1019
DpnII GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 432, 1116
EarI CTCTTC 2 cut(s) 432, 1116
Ecl136II GAGCTC 4 cut(s) 57, 513, 741, 969
Eco130I CCWWGG 2 cut(s) 165, 270
Eco24I GRGCYC 4 cut(s) 59, 515, 743, 971
Eco47I GGWCC 3 cut(s) 343, 571, 1027
Eco53kI GAGCTC 4 cut(s) 57, 513, 741, 969
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 4 cut(s) 57, 513, 741, 969
EcoRII CCWGG 2 cut(s) 90, 318
EcoT14I CCWWGG 2 cut(s) 165, 270
EcoT38I GRGCYC 4 cut(s) 59, 515, 743, 971
ErhI CCWWGG 2 cut(s) 165, 270
Esp3I CGTCTC 2 cut(s) 449, 905
FaiI YATR 5 cut(s) 230, 458, 686, 815, 914
FblI GTMKAC 3 cut(s) 422, 878, 1106
Fnu4HI GCNGC 5 cut(s) 146, 160, 374, 602, 1058
FokI GGATG 6 cut(s) 167, 623, 623, 851, 1079, 1079
FriOI GRGCYC 4 cut(s) 59, 515, 743, 971
Fsp4HI GCNGC 5 cut(s) 146, 160, 374, 602, 1058
FspBI CTAG 2 cut(s) 149, 605
GluI GCNGC 5 cut(s) 146, 160, 374, 602, 1058
GsaI CCCAGC 1 cut(s) 1053
HaeIII GGCC 1 cut(s) 159
HapII CCGG 5 cut(s) 341, 569, 594, 939, 1025
HgaI GACGC 1 cut(s) 852
HincII GTYRAC 2 cut(s) 651, 1107
HindII GTYRAC 2 cut(s) 651, 1107
HindIII AAGCTT 1 cut(s) 1141
HpaII CCGG 5 cut(s) 341, 569, 594, 939, 1025
HphI GGTGA 2 cut(s) 422, 487
Hpy166II GTNNAC 4 cut(s) 423, 651, 879, 1107
Hpy8I GTNNAC 4 cut(s) 423, 651, 879, 1107
HpyAV CCTTC 8 cut(s) 95, 323, 381, 449, 665, 779, 1007, 1093
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 6 cut(s) 4, 232, 460, 688, 916, 1082
HpyF3I CTNAG 7 cut(s) 212, 514, 540, 674, 742, 970, 996
HpySE526I ACGT 1 cut(s) 303
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
Lsp1109I GCAGC 4 cut(s) 157, 385, 613, 1069
MaeI CTAG 2 cut(s) 149, 605
MaeII ACGT 1 cut(s) 303
MalI GATC 7 cut(s) 89, 131, 236, 317, 545, 801, 1019
MboI GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
MfeI CAATTG 1 cut(s) 978
MflI RGATCY 5 cut(s) 87, 234, 315, 543, 1017
MhlI GDGCHC 4 cut(s) 59, 515, 743, 971
MluCI AATT 2 cut(s) 6, 978
MmeI TCCRAC 1 cut(s) 1052
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 2 cut(s) 105, 789
MseI TTAA 3 cut(s) 78, 218, 707
MspI CCGG 5 cut(s) 341, 569, 594, 939, 1025
MspR9I CCNGG 2 cut(s) 92, 320
MunI CAATTG 1 cut(s) 978
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 2 cut(s) 92, 320
NdeII GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
NlaIV GGNNCC 1 cut(s) 1019
PaeR7I CTCGAG 1 cut(s) 44
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 2 cut(s) 105, 789
PfeI GAWTC 6 cut(s) 191, 333, 561, 772, 790, 1000
PflFI GACNNNGTC 2 cut(s) 302, 530
PfoI TCCNGGA 2 cut(s) 90, 318
PkrI GCNGC 5 cut(s) 147, 161, 375, 603, 1059
Psp124BI GAGCTC 4 cut(s) 59, 515, 743, 971
Psp6I CCWGG 2 cut(s) 90, 318
PspFI CCCAGC 1 cut(s) 1049
PspGI CCWGG 2 cut(s) 90, 318
PspN4I GGNNCC 1 cut(s) 1019
PspPI GGNCC 3 cut(s) 343, 571, 1027
PsuI RGATCY 5 cut(s) 87, 234, 315, 543, 1017
PsyI GACNNNGTC 2 cut(s) 302, 530
SacI GAGCTC 4 cut(s) 59, 515, 743, 971
SalI GTCGAC 1 cut(s) 1105
SaqAI TTAA 3 cut(s) 78, 218, 707
SatI GCNGC 5 cut(s) 146, 160, 374, 602, 1058
Sau3AI GATC 7 cut(s) 87, 129, 234, 315, 543, 799, 1017
Sau96I GGNCC 3 cut(s) 343, 571, 1027
ScrFI CCNGG 2 cut(s) 92, 320
SduI GDGCHC 4 cut(s) 59, 515, 743, 971
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 3 cut(s) 343, 571, 1027
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 4 cut(s) 44, 500, 956, 1061
SmoI CTYRAG 4 cut(s) 44, 500, 956, 1061
Sse9I AATT 2 cut(s) 6, 978
SsiI CCGC 1 cut(s) 160
SspMI CTAG 2 cut(s) 149, 605
SstI GAGCTC 4 cut(s) 59, 515, 743, 971
StyD4I CCNGG 2 cut(s) 90, 318
StyI CCWWGG 2 cut(s) 165, 270
TaiI ACGT 1 cut(s) 306
TaqI TCGA 4 cut(s) 45, 69, 753, 1106
TasI AATT 2 cut(s) 6, 978
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 6 cut(s) 191, 333, 561, 772, 790, 1000
Tru1I TTAA 3 cut(s) 78, 218, 707
Tru9I TTAA 3 cut(s) 78, 218, 707
TscAI CASTG 1 cut(s) 31
TseI GCWGC 4 cut(s) 145, 373, 601, 1057
TspDTI ATGAA 1 cut(s) 647
TspGWI ACGGA 2 cut(s) 437, 893
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 2 cut(s) 302, 530
VpaK11BI GGWCC 3 cut(s) 343, 571, 1027
XapI RAATTY 1 cut(s) 6
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 3 cut(s) 422, 878, 1106
XmnI GAANNNNTTC 2 cut(s) 105, 789
XspI CTAG 2 cut(s) 149, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.