Rh3AG149900

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0017038
Physical Location & Seq
Reverse (-)
13287159 .. 13290784
3626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG149900.1

Sequence Viewer

Length: 1149 bp
ATGCAGATCTTCGTGAAAACCCTCACCGGCAAGACCATCACCCTCGAGGTCGAGAGCTCCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGGCATCCCTCCGGACCAGCAAAGGCTCATCTTCGCCGGAAAGCAGCTCGAGGACGGCCGCACCCTCGCCGACTACAACATCCAGAAGGAGTCCACCCTCCACCTGGTGCTCCGTCTCAGGGGAGGCATGCAGATCTTCGTCAAAACCCTCACCGGGAAGACGATCACTCTCGAGGTCGAGAGCTCCGACACCATCGATAACGTCAAGGCCAAGATCCAGGACAAGGAAGGGATCCCCCCGGACCAGCAGAGGCTGATCTTCGCCGGAAAGCAACTCGAAGACGGCAGGACTCTAGCCGATTACAACATCCAGAAGGAATCGACTCTCCATCTGGTGCTCCGTCTCAGGGGTGGTATGCAGATCTTCGTCAAAACCCTTACCGGCAAGACCATCACTCTCGAGGTTGAGAGCTCCGACACCATCGACAACGTCAAGGCTAAGATCCAGGACAAGGAGGGAATTCCCCCGGACCAGCAGAGGTTGATCTTCGCCGGAAAACAATTGGAGGACGGCAGGACCCTGGCCGATTACAACATCCAGAAGGAGTCGACTCTGCACTTGGTGCTCCGGTTGAGGGGTGGCATGCAGATCTTCGTCAAGACTTTGACGGGGAAGACCATCACCCTGGAGGTGGAGAGCTCCGACACGATCGACAACGTCAAGGCCAAGATTCAGGATAAGGAGGGGATTCCCCCGGATCAGCAGAGGTTGATCTTTGCTGGAAAGCAGTTGGAGGATGGCAGGACCCTGGCGGATTACAATATCCAGAAGGAGTCCACTCTTCATCTTGTCCTCAGGCTTCGCGGTGGTATGCAGATTTTCGTGAAGACTCTGACTGGCAAGACAATTACTCTGGAGGTGGAGAGCTCCGATACCATTGATAATGTCAAAGCCAAGATCCAGGACAAGGAAGGGATCCCACCGGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTCGAGGATGGTCGTACCCTGGCTGATTATAATATCCAGAAGGAGTCCACCCTCCACTTGGTCCTCCGTCTCCGTGGTGGTGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

382

Amino Acids

42.81

Weight (kDa)

6.58

Isoelectric Point (pI)

28.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 77 - 147 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 153 - 223 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 229 - 299 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 305 - 375 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 307 - 378 1.1e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017038)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20620
fragaria_vesca FvH4_3g21853
prunus_persica Prupe.4G025900_v2.0.a1 Prupe.6G238900_v2.0.a1
pyrus_communis pycom12g11970
rosa_chinensis RchiOBHm_Chr3g0469331
rosa_laevigata RLG00000024359 RLG00000031189 RLG00000033799
rosa_samantha Rh3AG149900 Rh3BG173500 Rh7AG256200 Rh7CG272600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AbsI CCTCGAGG 1 cut(s) 44
AccI GTMKAC 1 cut(s) 650
AccII CGCG 1 cut(s) 906
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 3 cut(s) 160, 854, 906
AclWI GGATC 9 cut(s) 82, 310, 328, 341, 538, 807, 994, 1012, 1025
AcoI YGGCCR 2 cut(s) 157, 624
AcsI RAATTY 1 cut(s) 561
AdeI CACNNNGTG 2 cut(s) 208, 664
AfaI GTAC 1 cut(s) 1075
AjnI CCWGG 9 cut(s) 90, 204, 318, 546, 621, 726, 849, 1002, 1077
AluBI AGCT 7 cut(s) 57, 148, 285, 513, 741, 969, 1060
AluI AGCT 7 cut(s) 57, 148, 285, 513, 741, 969, 1060
Alw21I GWGCWC 8 cut(s) 59, 213, 287, 441, 515, 669, 743, 971
Alw26I GTCTC 3 cut(s) 221, 449, 1133
AlwI GGATC 9 cut(s) 82, 310, 328, 341, 538, 807, 994, 1012, 1025
AlwNI CAGNNNCTG 2 cut(s) 355, 1039
Ama87I CYCGRG 5 cut(s) 44, 149, 272, 500, 1061
Aor13HI TCCGGA 1 cut(s) 112
AoxI GGCC 5 cut(s) 81, 157, 309, 624, 765
ApeKI GCWGC 2 cut(s) 145, 1057
ApoI RAATTY 1 cut(s) 561
AspS9I GGNCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
AsuC2I CCSGG 4 cut(s) 256, 341, 569, 797
AsuHPI GGTGA 4 cut(s) 16, 31, 244, 715
AvaI CYCGRG 5 cut(s) 44, 149, 272, 500, 1061
AvaII GGWCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
AxyI CCTNAGG 1 cut(s) 896
BamHI GGATCC 2 cut(s) 333, 1017
BanII GRGCYC 5 cut(s) 59, 287, 515, 743, 971
BbsI GAAGAC 4 cut(s) 266, 387, 722, 935
Bbv12I GWGCWC 8 cut(s) 59, 213, 287, 441, 515, 669, 743, 971
BbvI GCAGC 2 cut(s) 157, 1069
BccI CCATC 9 cut(s) 44, 74, 302, 438, 500, 530, 728, 833, 1061
BceAI ACGGC 3 cut(s) 172, 400, 628
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciT130I CCWGG 9 cut(s) 92, 206, 320, 548, 623, 728, 851, 1004, 1079
BcnI CCSGG 4 cut(s) 256, 341, 569, 797
BcoDI GTCTC 3 cut(s) 221, 449, 1133
BfaI CTAG 2 cut(s) 395, 1147
BglII AGATCT 4 cut(s) 6, 234, 462, 690
BisI GCNGC 3 cut(s) 146, 160, 1058
BlsI GCNGC 3 cut(s) 147, 161, 1059
Bme18I GGWCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
BmeT110I CYCGRG 5 cut(s) 44, 149, 272, 500, 1061
BmgT120I GGNCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
BmiI GGNNCC 4 cut(s) 335, 620, 848, 1019
BmsI GCATC 1 cut(s) 114
BpiI GAAGAC 4 cut(s) 266, 387, 722, 935
BpmI CTGGAG 2 cut(s) 749, 977
BpuMI CCSGG 4 cut(s) 256, 341, 569, 797
Bsa29I ATCGAT 1 cut(s) 297
BsaJI CCNNGG 8 cut(s) 339, 567, 621, 726, 795, 849, 1077, 1132
BsaWI WCCGGW 3 cut(s) 112, 669, 1024
Bse118I RCCGGY 3 cut(s) 26, 482, 1049
Bse1I ACTGG 1 cut(s) 943
Bse21I CCTNAGG 1 cut(s) 896
BseAI TCCGGA 1 cut(s) 112
BseBI CCWGG 9 cut(s) 92, 206, 320, 548, 623, 728, 851, 1004, 1079
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 8 cut(s) 339, 567, 621, 726, 795, 849, 1077, 1132
BseGI GGATG 6 cut(s) 105, 180, 408, 636, 844, 1072
BseMII CTCAG 3 cut(s) 232, 460, 910
BseNI ACTGG 1 cut(s) 943
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 2 cut(s) 157, 1069
BsgI GTGCAG 1 cut(s) 641
Bsh1236I CGCG 1 cut(s) 906
Bsh1285I CGRYCG 2 cut(s) 160, 753
BshFI GGCC 5 cut(s) 83, 159, 311, 626, 767
BshVI ATCGAT 1 cut(s) 297
BsiEI CGRYCG 2 cut(s) 160, 753
BsiHKAI GWGCWC 8 cut(s) 59, 213, 287, 441, 515, 669, 743, 971
BsiHKCI CYCGRG 5 cut(s) 44, 149, 272, 500, 1061
BsmAI GTCTC 3 cut(s) 221, 449, 1133
BsmBI CGTCTC 3 cut(s) 221, 449, 1133
BsnI GGCC 5 cut(s) 83, 159, 311, 626, 767
BsoBI CYCGRG 5 cut(s) 44, 149, 272, 500, 1061
Bsp1286I GDGCHC 8 cut(s) 59, 213, 287, 441, 515, 669, 743, 971
Bsp13I TCCGGA 1 cut(s) 112
BspACI CCGC 3 cut(s) 160, 854, 906
BspANI GGCC 5 cut(s) 83, 159, 311, 626, 767
BspCNI CTCAG 3 cut(s) 231, 459, 909
BspDI ATCGAT 1 cut(s) 297
BspEI TCCGGA 1 cut(s) 112
BspFNI CGCG 1 cut(s) 906
BspLI GGNNCC 4 cut(s) 335, 620, 848, 1019
BspPI GGATC 9 cut(s) 82, 310, 328, 341, 538, 807, 994, 1012, 1025
BsrFI RCCGGY 3 cut(s) 26, 482, 1049
BsrI ACTGG 1 cut(s) 943
BssAI RCCGGY 3 cut(s) 26, 482, 1049
BssECI CCNNGG 8 cut(s) 339, 567, 621, 726, 795, 849, 1077, 1132
Bst2UI CCWGG 9 cut(s) 92, 206, 320, 548, 623, 728, 851, 1004, 1079
Bst6I CTCTTC 1 cut(s) 888
BstAPI GCANNNNNTGC 1 cut(s) 664
BstC8I GCNNGC 4 cut(s) 230, 686, 1051, 1055
BstDEI CTNAG 4 cut(s) 218, 446, 540, 896
BstDSI CCRYGG 1 cut(s) 1132
BstF5I GGATG 6 cut(s) 105, 180, 408, 636, 844, 1072
BstFNI CGCG 1 cut(s) 906
BstMAI GTCTC 3 cut(s) 221, 449, 1133
BstMCI CGRYCG 2 cut(s) 160, 753
BstMWI GCNNNNNNNGC 1 cut(s) 664
BstNI CCWGG 9 cut(s) 92, 206, 320, 548, 623, 728, 851, 1004, 1079
BstNSI RCATGY 2 cut(s) 232, 688
BstUI CGCG 1 cut(s) 906
BstV1I GCAGC 2 cut(s) 157, 1069
BstV2I GAAGAC 4 cut(s) 266, 387, 722, 935
BstXI CCANNNNNNTGG 1 cut(s) 727
BstZI CGGCCG 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 297
Bsu36I CCTNAGG 1 cut(s) 896
BsuRI GGCC 5 cut(s) 83, 159, 311, 626, 767
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 1132
BtsCI GGATG 6 cut(s) 105, 180, 408, 636, 844, 1072
Cac8I GCNNGC 4 cut(s) 230, 686, 1051, 1055
CaiI CAGNNNCTG 2 cut(s) 355, 1039
Cfr10I RCCGGY 3 cut(s) 26, 482, 1049
Cfr13I GGNCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
ClaI ATCGAT 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 204
Csp6I GTAC 1 cut(s) 1074
CviAII CATG 2 cut(s) 229, 685
CviQI GTAC 1 cut(s) 1074
DdeI CTNAG 4 cut(s) 218, 446, 540, 896
DraIII CACNNNGTG 2 cut(s) 208, 664
EaeI YGGCCR 2 cut(s) 157, 624
EagI CGGCCG 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
EciI GGCGGA 1 cut(s) 869
Ecl136II GAGCTC 5 cut(s) 57, 285, 513, 741, 969
EclXI CGGCCG 1 cut(s) 157
Eco24I GRGCYC 5 cut(s) 59, 287, 515, 743, 971
Eco47I GGWCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 5 cut(s) 57, 285, 513, 741, 969
Eco81I CCTNAGG 1 cut(s) 896
Eco88I CYCGRG 5 cut(s) 44, 149, 272, 500, 1061
EcoICRI GAGCTC 5 cut(s) 57, 285, 513, 741, 969
EcoO109I RGGNCCY 2 cut(s) 618, 846
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 9 cut(s) 90, 204, 318, 546, 621, 726, 849, 1002, 1077
EcoT38I GRGCYC 5 cut(s) 59, 287, 515, 743, 971
Esp3I CGTCTC 3 cut(s) 221, 449, 1133
FaeI CATG 2 cut(s) 232, 688
FaiI YATR 5 cut(s) 230, 458, 686, 914, 1089
FatI CATG 2 cut(s) 228, 684
FblI GTMKAC 1 cut(s) 650
Fnu4HI GCNGC 3 cut(s) 146, 160, 1058
FokI GGATG 6 cut(s) 92, 167, 395, 623, 851, 1079
FriOI GRGCYC 5 cut(s) 59, 287, 515, 743, 971
Fsp4HI GCNGC 3 cut(s) 146, 160, 1058
FspBI CTAG 2 cut(s) 395, 1147
GluI GCNGC 3 cut(s) 146, 160, 1058
GsuI CTGGAG 2 cut(s) 749, 977
HaeIII GGCC 5 cut(s) 83, 159, 311, 626, 767
Hin1II CATG 2 cut(s) 232, 688
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HphI GGTGA 4 cut(s) 16, 31, 244, 715
Hpy166II GTNNAC 4 cut(s) 195, 651, 879, 1107
Hpy188I TCNGA 6 cut(s) 61, 289, 517, 745, 936, 973
Hpy8I GTNNAC 4 cut(s) 195, 651, 879, 1107
HpyAV CCTTC 7 cut(s) 181, 323, 409, 637, 865, 1007, 1093
HpyCH4IV ACGT 4 cut(s) 75, 303, 531, 759
HpyCH4V TGCA 6 cut(s) 4, 232, 460, 658, 688, 916
HpyF10VI GCNNNNNNNGC 1 cut(s) 664
HpyF3I CTNAG 4 cut(s) 218, 446, 540, 896
HpySE526I ACGT 4 cut(s) 75, 303, 531, 759
Hsp92II CATG 2 cut(s) 232, 688
Kpn2I TCCGGA 1 cut(s) 112
KroI GCCGGC 1 cut(s) 1049
KroNI GCCGGC 1 cut(s) 1051
LmnI GCTCC 8 cut(s) 62, 216, 290, 444, 518, 672, 746, 974
Lsp1109I GCAGC 2 cut(s) 157, 1069
LweI GCATC 1 cut(s) 114
MabI ACCWGGT 1 cut(s) 204
MaeI CTAG 2 cut(s) 395, 1147
MaeII ACGT 4 cut(s) 75, 303, 531, 759
MfeI CAATTG 1 cut(s) 602
MhlI GDGCHC 8 cut(s) 59, 213, 287, 441, 515, 669, 743, 971
MluCI AATT 3 cut(s) 561, 602, 948
MlyI GAGTC 8 cut(s) 200, 385, 418, 646, 656, 884, 925, 1112
MmeI TCCRAC 5 cut(s) 84, 312, 540, 768, 813
MroI TCCGGA 1 cut(s) 112
MroNI GCCGGC 1 cut(s) 1049
MunI CAATTG 1 cut(s) 602
MvaI CCWGG 9 cut(s) 92, 206, 320, 548, 623, 728, 851, 1004, 1079
MvnI CGCG 1 cut(s) 906
MwoI GCNNNNNNNGC 1 cut(s) 664
NaeI GCCGGC 1 cut(s) 1051
NciI CCSGG 4 cut(s) 256, 341, 569, 797
NgoMIV GCCGGC 1 cut(s) 1049
NlaIII CATG 2 cut(s) 232, 688
NlaIV GGNNCC 4 cut(s) 335, 620, 848, 1019
NspI RCATGY 2 cut(s) 232, 688
PaeI GCATGC 2 cut(s) 232, 688
PaeR7I CTCGAG 5 cut(s) 44, 149, 272, 500, 1061
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 285
PdiI GCCGGC 1 cut(s) 1051
PfeI GAWTC 3 cut(s) 419, 772, 790
PflFI GACNNNGTC 3 cut(s) 74, 530, 758
PfoI TCCNGGA 4 cut(s) 90, 318, 546, 1002
PkrI GCNGC 3 cut(s) 147, 161, 1059
Ple19I CGATCG 1 cut(s) 753
PleI GAGTC 8 cut(s) 199, 385, 418, 646, 655, 883, 925, 1111
PpsI GAGTC 8 cut(s) 199, 385, 418, 646, 655, 883, 925, 1111
PpuMI RGGWCCY 2 cut(s) 618, 846
PsiI TTATAA 1 cut(s) 1089
Psp124BI GAGCTC 5 cut(s) 59, 287, 515, 743, 971
Psp5II RGGWCCY 2 cut(s) 618, 846
Psp6I CCWGG 9 cut(s) 90, 204, 318, 546, 621, 726, 849, 1002, 1077
PspGI CCWGG 9 cut(s) 90, 204, 318, 546, 621, 726, 849, 1002, 1077
PspN4I GGNNCC 4 cut(s) 335, 620, 848, 1019
PspPI GGNCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
PspPPI RGGWCCY 2 cut(s) 618, 846
PspXI VCTCGAGB 3 cut(s) 44, 149, 1061
PstNI CAGNNNCTG 2 cut(s) 355, 1039
PsyI GACNNNGTC 3 cut(s) 74, 530, 758
PvuI CGATCG 1 cut(s) 753
RsaI GTAC 1 cut(s) 1075
RsaNI GTAC 1 cut(s) 1074
SacI GAGCTC 5 cut(s) 59, 287, 515, 743, 971
SalI GTCGAC 1 cut(s) 649
SatI GCNGC 3 cut(s) 146, 160, 1058
Sau96I GGNCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
SchI GAGTC 8 cut(s) 200, 385, 418, 646, 656, 884, 925, 1112
SduI GDGCHC 8 cut(s) 59, 213, 287, 441, 515, 669, 743, 971
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 5 cut(s) 44, 149, 272, 500, 1061
SinI GGWCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
SlaI CTCGAG 5 cut(s) 44, 149, 272, 500, 1061
SmlI CTYRAG 5 cut(s) 44, 149, 272, 500, 1061
SmoI CTYRAG 5 cut(s) 44, 149, 272, 500, 1061
SphI GCATGC 2 cut(s) 232, 688
Sse9I AATT 3 cut(s) 561, 602, 948
SsiI CCGC 3 cut(s) 160, 854, 906
SspMI CTAG 2 cut(s) 395, 1147
SstI GAGCTC 5 cut(s) 59, 287, 515, 743, 971
TaiI ACGT 4 cut(s) 78, 306, 534, 762
TasI AATT 3 cut(s) 561, 602, 948
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 3 cut(s) 419, 772, 790
TseI GCWGC 2 cut(s) 145, 1057
TspDTI ATGAA 1 cut(s) 875
TspGWI ACGGA 4 cut(s) 203, 431, 1115, 1121
Tth111I GACNNNGTC 3 cut(s) 74, 530, 758
VpaK11BI GGWCC 7 cut(s) 115, 343, 571, 618, 846, 1027, 1120
XapI RAATTY 1 cut(s) 561
XceI RCATGY 2 cut(s) 232, 688
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 1114
XhoI CTCGAG 5 cut(s) 44, 149, 272, 500, 1061
XmiI GTMKAC 1 cut(s) 650
XspI CTAG 2 cut(s) 395, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.