pycom12g11970

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017038
Physical Location & Seq
Forward (+)
14424516 .. 14426139
1624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11970.1

Sequence Viewer

Length: 1167 bp
ATGCAGATCTTCGTGAAAACCCTAACCGGCAAGACCATCACTCTCGAGGTCGAGAGCTCCGACACGATCGATAACGTCAAGGCTAAGATCCAGGATAAGGAAGGCATCCCTCCCGATCAGCAGCGGCTTATCTTCGCCGGAAAGCAGCTCGAGGATGGCCGGACCCTTGCCGATTACAATATCCAGAAGGAATCGACTCTCCATCTGTCGACTTTGCACTTGGTTCTTCGATTGAGAGGCGGTATGCAGATCTTTGTCAAAACCCTGACCGGGAAGACAATTACTCTGGAGGTCGAAAGTTCTGATACGATCGATAACGTCAAGGCAAAGATCCAAGATAAGGAGGGAATCCCCCCAGATCAGCAGAGATTAATTTTTGCTGGTAAGCAATTGGAGGATGGTAGGACTCTTGGGGATTACAACATCCAAAAGGAGTCAACTTTGCACTTGGTTCTTCGATTGAGGGGTGGTATGCAGATATTTGTGAAGACTCTGACTGGAAAGACTATCACTCTGGAGGTGGAGAGCTCAGATACCATTGATAACGTGAAGGCGAAGATTCAAGACAAGGAGGGCATTCCTCCAGATCAGCAGAGGCTTATTTTTGCCGGGAAGCAGTTGGAGGATGGCAGGACCCTGGCGGATTACAACATCCAGAAGGAGTCGACCCTTCACCTTGTGCTCAGGCTTCGCGGTGGTATGCAGATATTTGTGAAGACACTGACGGGGAAGACGATCACTCTCGAGGTTGAGAGCTCTGACACTATTGACAATGTCAAAGCTAAGATTCAGGACAAGGAGGGGATCCCACCTGACCAGCAAAGGTTGATCTTCGCTGGGAAACAGCTAGAGGATGGTCGTACCCTTGCGGATTACAACATTCAGAAAGAGTCTACCCTCCATCTCGTTCTTCGTCTCCGTGGCGTAATGACTTCATTTCTAACGATGTTGTTCTGCTGCTATAATTCAGTTAGCCTAATCCTGCTCATGAGGTTATTGGTTGTGCGTGAATTAAAAAGGGAATGGATTGGATTAAACAATGAATTTGTGTGCATCTGTTGTGGCTTAAGAAGTAGCAGCAAGCGATGGATAGTAATTGAAATACTCAAATTAGGTTTAGTGAATTCCATCTCGAAGGGATGTTCTGATCTGATCTGTATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

389

Amino Acids

43.66

Weight (kDa)

8.34

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 69 5e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 70 1.3e-29 Ubiquitin family
Rad60-SLD PF11976 82 - 152 9.1e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 84 - 155 9.3e-32 Ubiquitin family
Rad60-SLD PF11976 158 - 228 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 160 - 231 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 234 - 304 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 236 - 307 1.1e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017038)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20620
fragaria_vesca FvH4_3g21853
prunus_persica Prupe.4G025900_v2.0.a1 Prupe.6G238900_v2.0.a1
pyrus_communis pycom12g11970
rosa_chinensis RchiOBHm_Chr3g0469331
rosa_laevigata RLG00000024359 RLG00000031189 RLG00000033799
rosa_samantha Rh3AG149900 Rh3BG173500 Rh7AG256200 Rh7CG272600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 209, 665, 893
AccII CGCG 1 cut(s) 693
AciI CCGC 5 cut(s) 124, 240, 641, 693, 869
AclWI GGATC 4 cut(s) 82, 325, 799, 812
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 2 cut(s) 1043, 1123
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 1 cut(s) 862
AfiI CCNNNNNNNGG 3 cut(s) 97, 270, 340
AflII CTTAAG 1 cut(s) 1066
AgsI TTSAA 2 cut(s) 563, 1100
AjnI CCWGG 2 cut(s) 90, 636
AluBI AGCT 6 cut(s) 57, 148, 528, 756, 782, 847
AluI AGCT 6 cut(s) 57, 148, 528, 756, 782, 847
Alw21I GWGCWC 4 cut(s) 59, 530, 684, 758
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 4 cut(s) 82, 325, 799, 812
Ama87I CYCGRG 3 cut(s) 44, 149, 743
AoxI GGCC 1 cut(s) 157
ApeKI GCWGC 4 cut(s) 121, 145, 957, 1077
ApoI RAATTY 2 cut(s) 1043, 1123
AseI ATTAAT 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 162, 633
AsuC2I CCSGG 2 cut(s) 271, 610
AsuHPI GGTGA 1 cut(s) 665
AvaI CYCGRG 3 cut(s) 44, 149, 743
AvaII GGWCC 2 cut(s) 162, 633
BamHI GGATCC 1 cut(s) 804
BanII GRGCYC 3 cut(s) 59, 530, 758
BbsI GAAGAC 4 cut(s) 281, 494, 722, 737
Bbv12I GWGCWC 4 cut(s) 59, 530, 684, 758
BbvI GCAGC 4 cut(s) 133, 157, 944, 1089
BccI CCATC 9 cut(s) 44, 149, 210, 392, 620, 848, 909, 1080, 1136
BciT130I CCWGG 2 cut(s) 92, 638
BcnI CCSGG 2 cut(s) 271, 610
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 1 cut(s) 848
BfrI CTTAAG 1 cut(s) 1066
BglII AGATCT 2 cut(s) 6, 249
BisI GCNGC 5 cut(s) 122, 125, 146, 958, 1078
BlsI GCNGC 5 cut(s) 123, 126, 147, 959, 1079
Bme1390I CCNGG 4 cut(s) 92, 271, 610, 638
Bme18I GGWCC 2 cut(s) 162, 633
BmeT110I CYCGRG 3 cut(s) 44, 149, 743
BmgT120I GGNCC 2 cut(s) 162, 633
BmiI GGNNCC 3 cut(s) 164, 635, 806
BmrFI CCNGG 4 cut(s) 92, 271, 610, 638
BmsI GCATC 2 cut(s) 114, 1062
BpiI GAAGAC 4 cut(s) 281, 494, 722, 737
BpmI CTGGAG 3 cut(s) 308, 536, 567
Bpu10I CCTNAGC 1 cut(s) 683
BpuMI CCSGG 2 cut(s) 271, 610
Bsa29I ATCGAT 2 cut(s) 69, 312
BsaJI CCNNGG 2 cut(s) 636, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 270, 340
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 502
BseBI CCWGG 2 cut(s) 92, 638
BseCI ATCGAT 2 cut(s) 69, 312
BseDI CCNNGG 2 cut(s) 636, 919
BseGI GGATG 8 cut(s) 105, 160, 403, 423, 631, 651, 859, 1145
BseLI CCNNNNNNNGG 3 cut(s) 97, 270, 340
BseMII CTCAG 2 cut(s) 543, 697
BseNI ACTGG 1 cut(s) 502
BseXI GCAGC 4 cut(s) 133, 157, 944, 1089
BseYI CCCAGC 1 cut(s) 836
Bsh1236I CGCG 1 cut(s) 693
Bsh1285I CGRYCG 2 cut(s) 69, 312
BshFI GGCC 1 cut(s) 159
BshVI ATCGAT 2 cut(s) 69, 312
BsiEI CGRYCG 2 cut(s) 69, 312
BsiHKAI GWGCWC 4 cut(s) 59, 530, 684, 758
BsiHKCI CYCGRG 3 cut(s) 44, 149, 743
BsiSI CCGG 5 cut(s) 27, 138, 160, 270, 609
BslI CCNNNNNNNGG 3 cut(s) 97, 270, 340
BsmAI GTCTC 1 cut(s) 920
BsmBI CGTCTC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 3 cut(s) 44, 149, 743
Bsp1286I GDGCHC 4 cut(s) 59, 530, 684, 758
BspACI CCGC 5 cut(s) 124, 240, 641, 693, 869
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 2 cut(s) 542, 696
BspDI ATCGAT 2 cut(s) 69, 312
BspFNI CGCG 1 cut(s) 693
BspHI TCATGA 1 cut(s) 987
BspLI GGNNCC 3 cut(s) 164, 635, 806
BspPI GGATC 4 cut(s) 82, 325, 799, 812
BspTI CTTAAG 1 cut(s) 1066
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 636, 919
Bst2UI CCWGG 2 cut(s) 92, 638
BstAFI CTTAAG 1 cut(s) 1066
BstC8I GCNNGC 1 cut(s) 1082
BstDEI CTNAG 4 cut(s) 84, 529, 683, 783
BstDSI CCRYGG 1 cut(s) 919
BstF5I GGATG 8 cut(s) 105, 160, 403, 423, 631, 651, 859, 1145
BstFNI CGCG 1 cut(s) 693
BstMAI GTCTC 1 cut(s) 920
BstMCI CGRYCG 2 cut(s) 69, 312
BstNI CCWGG 2 cut(s) 92, 638
BstSCI CCNGG 4 cut(s) 90, 269, 608, 636
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 4 cut(s) 133, 157, 944, 1089
BstV2I GAAGAC 4 cut(s) 281, 494, 722, 737
BstX2I RGATCY 5 cut(s) 6, 87, 249, 330, 804
BstYI RGATCY 5 cut(s) 6, 87, 249, 330, 804
Bsu15I ATCGAT 2 cut(s) 69, 312
BsuRI GGCC 1 cut(s) 159
BsuTUI ATCGAT 2 cut(s) 69, 312
BtgI CCRYGG 1 cut(s) 919
BtgZI GCGATG 1 cut(s) 1099
BtsCI GGATG 8 cut(s) 105, 160, 403, 423, 631, 651, 859, 1145
BtsIMutI CAGTG 1 cut(s) 719
Cac8I GCNNGC 1 cut(s) 1082
CciI TCATGA 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 162, 633
ClaI ATCGAT 2 cut(s) 69, 312
Csp6I GTAC 1 cut(s) 861
CviAII CATG 1 cut(s) 988
CviQI GTAC 1 cut(s) 861
DdeI CTNAG 4 cut(s) 84, 529, 683, 783
DraIII CACNNNGTG 1 cut(s) 679
EaeI YGGCCR 1 cut(s) 157
EciI GGCGGA 1 cut(s) 656
Ecl136II GAGCTC 3 cut(s) 57, 528, 756
Eco24I GRGCYC 3 cut(s) 59, 530, 758
Eco47I GGWCC 2 cut(s) 162, 633
Eco53kI GAGCTC 3 cut(s) 57, 528, 756
Eco88I CYCGRG 3 cut(s) 44, 149, 743
EcoICRI GAGCTC 3 cut(s) 57, 528, 756
EcoO109I RGGNCCY 1 cut(s) 633
EcoRI GAATTC 1 cut(s) 1123
EcoRII CCWGG 2 cut(s) 90, 636
EcoT38I GRGCYC 3 cut(s) 59, 530, 758
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 1 cut(s) 991
FaiI YATR 6 cut(s) 245, 473, 701, 963, 989, 1160
FatI CATG 1 cut(s) 987
FblI GTMKAC 3 cut(s) 209, 665, 893
Fnu4HI GCNGC 5 cut(s) 122, 125, 146, 958, 1078
FokI GGATG 8 cut(s) 92, 167, 410, 410, 638, 638, 866, 1152
FriOI GRGCYC 3 cut(s) 59, 530, 758
Fsp4HI GCNGC 5 cut(s) 122, 125, 146, 958, 1078
FspBI CTAG 1 cut(s) 848
GluI GCNGC 5 cut(s) 122, 125, 146, 958, 1078
GsaI CCCAGC 1 cut(s) 840
GsuI CTGGAG 3 cut(s) 308, 536, 567
HaeIII GGCC 1 cut(s) 159
HapII CCGG 5 cut(s) 27, 138, 160, 270, 609
Hin1II CATG 1 cut(s) 991
HincII GTYRAC 3 cut(s) 210, 438, 666
HindII GTYRAC 3 cut(s) 210, 438, 666
HpaII CCGG 5 cut(s) 27, 138, 160, 270, 609
HphI GGTGA 1 cut(s) 665
Hpy166II GTNNAC 4 cut(s) 210, 438, 666, 894
Hpy188I TCNGA 8 cut(s) 61, 304, 495, 532, 760, 885, 1147, 1152
Hpy8I GTNNAC 4 cut(s) 210, 438, 666, 894
HpyAV CCTTC 6 cut(s) 95, 181, 544, 652, 680, 1129
HpyCH4IV ACGT 3 cut(s) 75, 318, 546
HpyCH4V TGCA 7 cut(s) 4, 217, 247, 445, 475, 703, 1053
HpyF3I CTNAG 4 cut(s) 84, 529, 683, 783
HpySE526I ACGT 3 cut(s) 75, 318, 546
Hsp92II CATG 1 cut(s) 991
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 4 cut(s) 133, 157, 944, 1089
LweI GCATC 2 cut(s) 114, 1062
MaeI CTAG 1 cut(s) 848
MaeII ACGT 3 cut(s) 75, 318, 546
MfeI CAATTG 1 cut(s) 389
MflI RGATCY 5 cut(s) 6, 87, 249, 330, 804
MhlI GDGCHC 4 cut(s) 59, 530, 684, 758
MluCI AATT 9 cut(s) 279, 372, 389, 964, 1010, 1043, 1095, 1109, 1123
MlyI GAGTC 6 cut(s) 190, 400, 443, 484, 671, 899
MmeI TCCRAC 2 cut(s) 84, 600
MseI TTAA 4 cut(s) 371, 1013, 1034, 1067
MspA1I CMGCKG 1 cut(s) 124
MspCI CTTAAG 1 cut(s) 1066
MspI CCGG 5 cut(s) 27, 138, 160, 270, 609
MspR9I CCNGG 4 cut(s) 92, 271, 610, 638
MunI CAATTG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 576
MvaI CCWGG 2 cut(s) 92, 638
MvnI CGCG 1 cut(s) 693
NciI CCSGG 2 cut(s) 271, 610
NlaIII CATG 1 cut(s) 991
NlaIV GGNNCC 3 cut(s) 164, 635, 806
PaeR7I CTCGAG 3 cut(s) 44, 149, 743
PagI TCATGA 1 cut(s) 987
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 731
PctI GAATGC 1 cut(s) 576
PfeI GAWTC 4 cut(s) 191, 348, 559, 787
PflFI GACNNNGTC 1 cut(s) 773
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 5 cut(s) 123, 126, 147, 959, 1079
Ple19I CGATCG 2 cut(s) 69, 312
PleI GAGTC 6 cut(s) 190, 400, 442, 484, 670, 898
PpsI GAGTC 6 cut(s) 190, 400, 442, 484, 670, 898
PpuMI RGGWCCY 1 cut(s) 633
PshBI ATTAAT 1 cut(s) 371
Psp124BI GAGCTC 3 cut(s) 59, 530, 758
Psp5II RGGWCCY 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 90, 636
PspFI CCCAGC 1 cut(s) 836
PspGI CCWGG 2 cut(s) 90, 636
PspN4I GGNNCC 3 cut(s) 164, 635, 806
PspPI GGNCC 2 cut(s) 162, 633
PspPPI RGGWCCY 1 cut(s) 633
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 5 cut(s) 6, 87, 249, 330, 804
PsyI GACNNNGTC 1 cut(s) 773
PvuI CGATCG 2 cut(s) 69, 312
RsaI GTAC 1 cut(s) 862
RsaNI GTAC 1 cut(s) 861
SacI GAGCTC 3 cut(s) 59, 530, 758
SalI GTCGAC 2 cut(s) 208, 664
SaqAI TTAA 4 cut(s) 371, 1013, 1034, 1067
SatI GCNGC 5 cut(s) 122, 125, 146, 958, 1078
Sau96I GGNCC 2 cut(s) 162, 633
SchI GAGTC 6 cut(s) 190, 400, 443, 484, 671, 899
ScrFI CCNGG 4 cut(s) 92, 271, 610, 638
SduI GDGCHC 4 cut(s) 59, 530, 684, 758
SfaNI GCATC 2 cut(s) 114, 1062
Sfr274I CTCGAG 3 cut(s) 44, 149, 743
SinI GGWCC 2 cut(s) 162, 633
SlaI CTCGAG 3 cut(s) 44, 149, 743
SmlI CTYRAG 4 cut(s) 44, 149, 743, 1066
SmoI CTYRAG 4 cut(s) 44, 149, 743, 1066
Sse9I AATT 9 cut(s) 279, 372, 389, 964, 1010, 1043, 1095, 1109, 1123
SsiI CCGC 5 cut(s) 124, 240, 641, 693, 869
SspMI CTAG 1 cut(s) 848
SstI GAGCTC 3 cut(s) 59, 530, 758
StyD4I CCNGG 4 cut(s) 90, 269, 608, 636
TaiI ACGT 3 cut(s) 78, 321, 549
TasI AATT 9 cut(s) 279, 372, 389, 964, 1010, 1043, 1095, 1109, 1123
TauI GCSGC 1 cut(s) 127
TfiI GAWTC 4 cut(s) 191, 348, 559, 787
Tru1I TTAA 4 cut(s) 371, 1013, 1034, 1067
Tru9I TTAA 4 cut(s) 371, 1013, 1034, 1067
TscAI CASTG 1 cut(s) 726
TseI GCWGC 4 cut(s) 121, 145, 957, 1077
TspDTI ATGAA 2 cut(s) 924, 1056
TspGWI ACGGA 1 cut(s) 908
TspRI CASTG 1 cut(s) 726
Tth111I GACNNNGTC 1 cut(s) 773
Vha464I CTTAAG 1 cut(s) 1066
VpaK11BI GGWCC 2 cut(s) 162, 633
VspI ATTAAT 1 cut(s) 371
XapI RAATTY 2 cut(s) 1043, 1123
XhoI CTCGAG 3 cut(s) 44, 149, 743
XmiI GTMKAC 3 cut(s) 209, 665, 893
XspI CTAG 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.