AT5G22070

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016215
Physical Location & Seq
Forward (+)
7307894 .. 7310367
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22070.1

Sequence Viewer

Length: 1089 bp
ATGTTCTCGAATCAGTTTGTTCTCTTCTTCTCTCTTCTCCTATGTCTCCCATTTGTTTTCTTCTTTGTAGCGCCACATGTGTTTCCTTCTCCTCCTGGTGAATCTTTAATCCCAATCTCCGATGAGATTGATGACCGGAGTTTATTCATCGCCGCTGCTGGATCAACCTCTCTTTCTCATCTCTCATCTGGAAACCCTAACCCTAAGCTCAAGATTGCTTTTCTATTCCTCACCAACTCCGATCTCCATTTCGCGCCAATCTGGGATCGATTCTTCTCTGGTCATTCGAAATCTCTCTACAACGTCTATGTCCACGCAGATCCTTTTGTCAATGTCACAAGACCTGGGAATGGCTCTGTGTTTGAGAATGCCTTCATTGCAAACGCGAAACGCACCGCAAGAGCTTCTCCGACTCTTATCTCCGCCACGCGGCGCCTCCTCGCTACCGCTTTCCTTGATGACCCTGCGAACACTTACTTCGCTGTGCTCTCTCAGTATTGTATTCCTCTCCACTCGTTCAACTATGTTTATAGCTCTCTCTTTGAATCTTCAATTTTTGATAAATCTGATCCGGATCCGAATCCGAATCCGAGAGGTATTAGAATCTTGTATCGGAGTTTTATGGAACTGATATCTGATGAGCCTAGGCTTTGGAAACGGTACACTGCTAGAGGAAGGTATGCTATGATGCCTGAGGTTCCGTTTGAGAAATTCAGAGTTGGTTCGCAGTTCTTTGTTATGACTAGAAGACATGCTTTGTTAACAATCAAAGATCGGATCTTGTGGCGTAAATTCAAGCTCCCGTGTTATCGTTCTGATGAGTGTTACCCGGAGGAGCATTATTTCCCGACGCTGTTGAACATGAAGGATCCGGATGGTTGCACTGGGTATACGTTGACGCGAGTTAACTGGACTGGGACTGTGAAAGGACATCCTTATACTTACAAACCAAAGGAAGTTGTGCCAGAGTTGATCCAGAGACTCCGGCGATCGAATCACTCTAGCTCCTACTTTTTCGCCCGCAAATTTACTCCGGATTGCTTGAAGCCGCTTTTGGCCATTGCAGATTCTGTGATTTTCAGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

362

Amino Acids

41.64

Weight (kDa)

9.27

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 72 - 190 1.7e-27 Core-2/I-Branching enzyme
Branch PF02485 206 - 329 1.2e-28 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22070
fragaria_vesca FvH4_2g09440
malus_domestica MD03G1158600.v1.1
prunus_persica Prupe.6G146600_v2.0.a1 Prupe.6G146600_v2.0.a1
pyrus_communis pycom03g11090
rosa_chinensis RchiOBHm_Chr5g0048971
rosa_laevigata RLG00000034575
rosa_multiflora Rmu_sc0004058.1_g000017
rosa_rugosa Rorug05G0241600
rosa_samantha Rh5AG321800 Rh5BG332000 Rh5CG357300 Rh5DG343800
rosa_wichuraiana Rw5G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 432
AccI GTMKAC 1 cut(s) 890
AccII CGCG 4 cut(s) 254, 386, 430, 901
AccIII TCCGGA 3 cut(s) 571, 871, 1033
AciI CCGC 7 cut(s) 153, 396, 423, 430, 447, 1021, 1049
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 3 cut(s) 710, 791, 1025
AcyI GRCGYC 1 cut(s) 433
AfaI GTAC 1 cut(s) 662
AfiI CCNNNNNNNGG 2 cut(s) 350, 429
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 6 cut(s) 520, 545, 552, 796, 859, 1045
AjnI CCWGG 2 cut(s) 94, 343
AjuI GAANNNNNNNTTGG 2 cut(s) 1037, 1069
AluBI AGCT 5 cut(s) 208, 404, 534, 799, 1005
AluI AGCT 5 cut(s) 208, 404, 534, 799, 1005
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 50, 973
AlwNI CAGNNNCTG 1 cut(s) 1070
Aor13HI TCCGGA 3 cut(s) 571, 871, 1033
AoxI GGCC 1 cut(s) 1056
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 3 cut(s) 710, 791, 1025
Asp700I GAANNNNTTC 1 cut(s) 371
AspA2I CCTAGG 1 cut(s) 644
AspLEI GCGC 3 cut(s) 73, 256, 435
AsuC2I CCSGG 1 cut(s) 830
AsuHPI GGTGA 2 cut(s) 110, 223
AsuII TTCGAA 1 cut(s) 287
AvrII CCTAGG 1 cut(s) 644
AxyI CCTNAGG 1 cut(s) 693
BalI TGGCCA 1 cut(s) 1058
BamHI GGATCC 2 cut(s) 574, 868
BanI GGYRCC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 754
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 869
BciT130I CCWGG 2 cut(s) 96, 345
BcnI CCSGG 1 cut(s) 830
BcoDI GTCTC 2 cut(s) 50, 973
BfaI CTAG 4 cut(s) 645, 669, 744, 1002
BfoI RGCGCY 2 cut(s) 74, 436
BisI GCNGC 4 cut(s) 153, 156, 431, 1049
BlnI CCTAGG 1 cut(s) 644
BlsI GCNGC 4 cut(s) 154, 157, 432, 1050
Bme1390I CCNGG 3 cut(s) 96, 345, 830
BmiI GGNNCC 4 cut(s) 434, 576, 699, 870
BmrFI CCNGG 3 cut(s) 96, 345, 830
BmrI ACTGGG 2 cut(s) 894, 924
BmsI GCATC 1 cut(s) 678
BmuI ACTGGG 2 cut(s) 894, 924
BpiI GAAGAC 1 cut(s) 754
Bpu10I CCTNAGC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 830
Bsa29I ATCGAT 1 cut(s) 268
BsaBI GATNNNNATC 2 cut(s) 573, 579
BsaHI GRCGYC 1 cut(s) 433
BsaJI CCNNGG 2 cut(s) 344, 644
BsaWI WCCGGW 4 cut(s) 135, 571, 871, 1033
BsaXI ACNNNNNCTCC 2 cut(s) 989, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 429
Bse1I ACTGG 3 cut(s) 889, 914, 919
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 2 cut(s) 375, 1059
Bse8I GATNNNNATC 2 cut(s) 573, 579
BseAI TCCGGA 3 cut(s) 571, 871, 1033
BseBI CCWGG 2 cut(s) 96, 345
BseCI ATCGAT 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 344, 644
BseGI GGATG 2 cut(s) 880, 931
BseJI GATNNNNATC 2 cut(s) 573, 579
BseLI CCNNNNNNNGG 2 cut(s) 350, 429
BseMI GCAATG 2 cut(s) 375, 1059
BseMII CTCAG 2 cut(s) 506, 684
BseNI ACTGG 3 cut(s) 889, 914, 919
BseRI GAGGAG 3 cut(s) 81, 428, 848
BseXI GCAGC 1 cut(s) 142
Bsh1236I CGCG 4 cut(s) 254, 386, 430, 901
Bsh1285I CGRYCG 1 cut(s) 992
BshFI GGCC 1 cut(s) 1058
BshNI GGYRCC 1 cut(s) 432
BshVI ATCGAT 1 cut(s) 268
BsiEI CGRYCG 1 cut(s) 992
BsiHKAI GWGCWC 1 cut(s) 489
BsiSI CCGG 6 cut(s) 136, 572, 830, 872, 985, 1034
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 2 cut(s) 350, 429
BsmAI GTCTC 2 cut(s) 50, 973
BsmFI GGGAC 1 cut(s) 931
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 1 cut(s) 1058
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 489
Bsp13I TCCGGA 3 cut(s) 571, 871, 1033
BspACI CCGC 7 cut(s) 153, 396, 423, 430, 447, 1021, 1049
BspANI GGCC 1 cut(s) 1058
BspCNI CTCAG 2 cut(s) 505, 685
BspDI ATCGAT 1 cut(s) 268
BspEI TCCGGA 3 cut(s) 571, 871, 1033
BspFNI CGCG 4 cut(s) 254, 386, 430, 901
BspLI GGNNCC 4 cut(s) 434, 576, 699, 870
BspT104I TTCGAA 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 432
BsrDI GCAATG 2 cut(s) 375, 1059
BsrI ACTGG 3 cut(s) 889, 914, 919
BssECI CCNNGG 2 cut(s) 344, 644
BssNAI GTATAC 1 cut(s) 891
BssNI GRCGYC 1 cut(s) 433
BssT1I CCWWGG 1 cut(s) 644
Bst1107I GTATAC 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 96, 345
Bst4CI ACNGT 2 cut(s) 660, 922
Bst6I CTCTTC 2 cut(s) 29, 39
BstACI GRCGYC 1 cut(s) 433
BstBI TTCGAA 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 1021
BstDEI CTNAG 3 cut(s) 204, 492, 693
BstF5I GGATG 2 cut(s) 880, 931
BstFNI CGCG 4 cut(s) 254, 386, 430, 901
BstH2I RGCGCY 2 cut(s) 74, 436
BstHHI GCGC 3 cut(s) 73, 256, 435
BstMAI GTCTC 2 cut(s) 50, 973
BstMCI CGRYCG 1 cut(s) 992
BstMWI GCNNNNNNNGC 1 cut(s) 377
BstNI CCWGG 2 cut(s) 96, 345
BstNSI RCATGY 2 cut(s) 80, 755
BstSCI CCNGG 3 cut(s) 94, 343, 828
BstUI CGCG 4 cut(s) 254, 386, 430, 901
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 754
BstX2I RGATCY 4 cut(s) 319, 574, 777, 868
BstYI RGATCY 4 cut(s) 319, 574, 777, 868
BstZ17I GTATAC 1 cut(s) 891
Bsu15I ATCGAT 1 cut(s) 268
Bsu36I CCTNAGG 1 cut(s) 693
BsuRI GGCC 1 cut(s) 1058
BsuTUI ATCGAT 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 880, 931
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 663, 882
Cac8I GCNNGC 1 cut(s) 1021
CaiI CAGNNNCTG 1 cut(s) 1070
CfoI GCGC 3 cut(s) 73, 256, 435
ClaI ATCGAT 1 cut(s) 268
CseI GACGC 2 cut(s) 859, 907
Csp6I GTAC 1 cut(s) 661
CviAII CATG 3 cut(s) 77, 752, 862
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 3 cut(s) 204, 492, 693
DinI GGCGCC 1 cut(s) 434
EaeI YGGCCR 1 cut(s) 1056
Eam1104I CTCTTC 2 cut(s) 29, 39
EarI CTCTTC 2 cut(s) 29, 39
EciI GGCGGA 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 644
Eco32I GATATC 1 cut(s) 633
Eco81I CCTNAGG 1 cut(s) 693
EcoRII CCWGG 2 cut(s) 94, 343
EcoRV GATATC 1 cut(s) 633
EcoT14I CCWWGG 1 cut(s) 644
EgeI GGCGCC 1 cut(s) 434
EheI GGCGCC 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 644
FaeI CATG 3 cut(s) 80, 755, 865
FalI AAGNNNNNCTT 2 cut(s) 739, 771
FaqI GGGAC 1 cut(s) 931
FatI CATG 3 cut(s) 76, 751, 861
FauI CCCGC 1 cut(s) 1028
FblI GTMKAC 1 cut(s) 890
Fnu4HI GCNGC 4 cut(s) 153, 156, 431, 1049
FokI GGATG 2 cut(s) 887, 918
Fsp4HI GCNGC 4 cut(s) 153, 156, 431, 1049
FspBI CTAG 4 cut(s) 645, 669, 744, 1002
GlaI GCGC 3 cut(s) 72, 255, 434
GluI GCNGC 4 cut(s) 153, 156, 431, 1049
HaeII RGCGCY 2 cut(s) 74, 436
HaeIII GGCC 1 cut(s) 1058
HapII CCGG 6 cut(s) 136, 572, 830, 872, 985, 1034
HgaI GACGC 2 cut(s) 859, 907
HhaI GCGC 3 cut(s) 73, 256, 435
Hin1I GRCGYC 1 cut(s) 433
Hin1II CATG 3 cut(s) 80, 755, 865
Hin6I GCGC 3 cut(s) 71, 254, 433
HinP1I GCGC 3 cut(s) 71, 254, 433
HincII GTYRAC 3 cut(s) 762, 897, 907
HindII GTYRAC 3 cut(s) 762, 897, 907
HpaI GTTAAC 2 cut(s) 762, 907
HpaII CCGG 6 cut(s) 136, 572, 830, 872, 985, 1034
HphI GGTGA 2 cut(s) 110, 223
Hpy166II GTNNAC 6 cut(s) 313, 663, 762, 891, 897, 907
Hpy188III TCNNGA 8 cut(s) 7, 189, 211, 572, 847, 872, 976, 1034
Hpy8I GTNNAC 6 cut(s) 313, 663, 762, 891, 897, 907
Hpy99I CGWCG 1 cut(s) 853
HpyAV CCTTC 4 cut(s) 96, 382, 669, 859
HpyCH4III ACNGT 2 cut(s) 660, 922
HpyCH4IV ACGT 2 cut(s) 303, 893
HpyCH4V TGCA 3 cut(s) 380, 882, 1064
HpyF10VI GCNNNNNNNGC 1 cut(s) 377
HpyF3I CTNAG 3 cut(s) 204, 492, 693
HpySE526I ACGT 2 cut(s) 303, 893
Hsp92I GRCGYC 1 cut(s) 433
Hsp92II CATG 3 cut(s) 80, 755, 865
HspAI GCGC 3 cut(s) 71, 254, 433
KasI GGCGCC 1 cut(s) 432
Kpn2I TCCGGA 3 cut(s) 571, 871, 1033
KspAI GTTAAC 2 cut(s) 762, 907
LmnI GCTCC 3 cut(s) 804, 835, 1010
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 678
MaeI CTAG 4 cut(s) 645, 669, 744, 1002
MaeII ACGT 2 cut(s) 303, 893
MaeIII GTNAC 2 cut(s) 334, 824
MboII GAAGA 7 cut(s) 16, 19, 26, 52, 265, 540, 759
MflI RGATCY 4 cut(s) 319, 574, 777, 868
MhlI GDGCHC 1 cut(s) 489
MlsI TGGCCA 1 cut(s) 1058
MluCI AATT 4 cut(s) 552, 710, 791, 1025
MluNI TGGCCA 1 cut(s) 1058
Mly113I GGCGCC 1 cut(s) 433
MlyI GAGTC 2 cut(s) 406, 975
MmeI TCCRAC 1 cut(s) 434
Mox20I TGGCCA 1 cut(s) 1058
MroI TCCGGA 3 cut(s) 571, 871, 1033
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 1058
MseI TTAA 4 cut(s) 107, 761, 906, 1087
Msp20I TGGCCA 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 6 cut(s) 136, 572, 830, 872, 985, 1034
MspR9I CCNGG 3 cut(s) 96, 345, 830
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 2 cut(s) 96, 345
MvnI CGCG 4 cut(s) 254, 386, 430, 901
MwoI GCNNNNNNNGC 1 cut(s) 377
NarI GGCGCC 1 cut(s) 433
NciI CCSGG 1 cut(s) 830
NlaIII CATG 3 cut(s) 80, 755, 865
NlaIV GGNNCC 4 cut(s) 434, 576, 699, 870
NmuCI GTSAC 1 cut(s) 334
NspI RCATGY 2 cut(s) 80, 755
NspV TTCGAA 1 cut(s) 287
PciI ACATGT 1 cut(s) 76
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 9 cut(s) 10, 101, 270, 545, 580, 586, 603, 994, 1067
PkrI GCNGC 4 cut(s) 154, 157, 432, 1050
Ple19I CGATCG 1 cut(s) 992
PleI GAGTC 2 cut(s) 406, 975
PluTI GGCGCC 1 cut(s) 436
PpsI GAGTC 2 cut(s) 406, 975
PscI ACATGT 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 94, 343
PspGI CCWGG 2 cut(s) 94, 343
PspN4I GGNNCC 4 cut(s) 434, 576, 699, 870
PstNI CAGNNNCTG 1 cut(s) 1070
PsuI RGATCY 4 cut(s) 319, 574, 777, 868
PvuI CGATCG 1 cut(s) 992
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
SaqAI TTAA 4 cut(s) 107, 761, 906, 1087
SatI GCNGC 4 cut(s) 153, 156, 431, 1049
SchI GAGTC 2 cut(s) 406, 975
ScrFI CCNGG 3 cut(s) 96, 345, 830
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 1 cut(s) 678
SfoI GGCGCC 1 cut(s) 434
SfuI TTCGAA 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 4 cut(s) 552, 710, 791, 1025
SsiI CCGC 7 cut(s) 153, 396, 423, 430, 447, 1021, 1049
SspDI GGCGCC 1 cut(s) 432
SspMI CTAG 4 cut(s) 645, 669, 744, 1002
StyD4I CCNGG 3 cut(s) 94, 343, 828
StyI CCWWGG 1 cut(s) 644
TaaI ACNGT 2 cut(s) 660, 922
TaiI ACGT 2 cut(s) 306, 896
TaqI TCGA 4 cut(s) 8, 268, 287, 992
TasI AATT 4 cut(s) 552, 710, 791, 1025
TauI GCSGC 3 cut(s) 155, 433, 1051
TfiI GAWTC 9 cut(s) 10, 101, 270, 545, 580, 586, 603, 994, 1067
Tru1I TTAA 4 cut(s) 107, 761, 906, 1087
Tru9I TTAA 4 cut(s) 107, 761, 906, 1087
TscAI CASTG 2 cut(s) 670, 889
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 3 cut(s) 136, 364, 878
TspGWI ACGGA 1 cut(s) 690
TspRI CASTG 2 cut(s) 670, 889
XapI RAATTY 3 cut(s) 710, 791, 1025
XceI RCATGY 2 cut(s) 80, 755
XmaJI CCTAGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 4 cut(s) 645, 669, 744, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.