Rw5G030370

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016215
Physical Location & Seq
Reverse (-)
48122463 .. 48123549
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030370.1

Sequence Viewer

Length: 1053 bp
ATGTTCTCCACCCCATTCGTACTCTCCTTCGCCCTCCTCCTCTCCATCCCTCTCCTCTTCCTCCTCGCCCCCCATTTCCTCCCCCCCAACAACCAAATCCCGATCCCCGCCGCCGATGAAATCGACGACCAGGCCCTCTTCTCCCGCGCCGTCCGATCCAAATCCGCATTCACCCACCTCTCCCTCGATTCCAAAACCAAGCCCAAAATCGCCTTCCTCTTCCTCACCAACTCCGACCTCCACTTCGCCCCCCTCTGGCAACGCTTCTTTTCCAAAACTGCCCCCAATCTCTACAATATTTACGTCCACGCCGATCCCTCCGTCAACGTCACCATCCCTCCCGGGGTTTTCCGCGACCGTTTCATCGCCTCAAAGCGGACCTACCGTGCCTCCCCGACCCTGATCTCCGCCACCCGCCGATTGCTCGCCACCGCCGTGCTCGACGATCCCGCCAATGTCTTCTTCGCCGTTCTCTCCCAGTACTGCGTCCCTCTCCATTCCTTCCCCTACGTCTACCGCTCCCTCTTCGCCTCCACCACTTTCGACAAGACCCGCCCCGCCACCAAGTCCGACGCCGAGTTGACTCGGATGGGATTGAAGTTCAGGTACAAGAGCTTCATCGAAATCCTCTCCAAATCGACCAGTCTCTGGAAGCGGTACGCCGCCAGGGGAAGGTACGCCATGATGCCGGAGGTCCCTTTCAACCAGTTCCGGGTCGGGTCGCAGTTCTTCTTACTCACGCGCCGTCACGCATTGGTGGTTCTGAAGGATCGGCAGCTGTGGCAGAAATTCAAATTGCCGTGTTATCGGGAGGACGAGTGTTACCCGGAGGAGCATTACTTTCCGACGCTGTTGTCGATGGCGGATCCGGATGGGTGTACCCATTATACTCTGACCCGGGTTAATTGGACGGGTACAGTGAAAGGGGCACCCCTTATTTATCAACTGCGGCAGTCAAATTTTTCCGAGTCTTACTTGTTTGCGAGGAAATTCTCGCCGGATTGCTTGAAACCCTTGTTGGGTTTATCCGAGAAGGTCATTTTCCGGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

350

Amino Acids

40.29

Weight (kDa)

9.72

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 70 - 180 1e-25 Core-2/I-Branching enzyme
Branch PF02485 202 - 314 4.1e-25 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22070
fragaria_vesca FvH4_2g09440
malus_domestica MD03G1158600.v1.1
prunus_persica Prupe.6G146600_v2.0.a1 Prupe.6G146600_v2.0.a1
pyrus_communis pycom03g11090
rosa_chinensis RchiOBHm_Chr5g0048971
rosa_laevigata RLG00000034575
rosa_multiflora Rmu_sc0004058.1_g000017
rosa_rugosa Rorug05G0241600
rosa_samantha Rh5AG321800 Rh5BG332000 Rh5CG357300 Rh5DG343800
rosa_wichuraiana Rw5G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 853
AccB1I GGYRCC 1 cut(s) 928
AccB7I CCANNNNNTGG 1 cut(s) 648
AccBSI CCGCTC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 513
AccII CGCG 3 cut(s) 147, 354, 742
AccIII TCCGGA 1 cut(s) 868
AclWI GGATC 7 cut(s) 97, 150, 308, 440, 777, 860, 873
AcsI RAATTY 3 cut(s) 788, 958, 989
AcuI CTGAAG 1 cut(s) 785
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 7 cut(s) 21, 482, 608, 659, 677, 880, 916
AfiI CCNNNNNNNGG 7 cut(s) 255, 343, 375, 648, 672, 712, 1019
AgsI TTSAA 4 cut(s) 598, 703, 793, 1009
AjnI CCWGG 2 cut(s) 129, 665
AjuI GAANNNNNNNTTGG 8 cut(s) 152, 184, 197, 229, 446, 478, 1001, 1033
AleI CACNNNNGTG 1 cut(s) 434
AluBI AGCT 2 cut(s) 615, 778
AluI AGCT 2 cut(s) 615, 778
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 7 cut(s) 97, 150, 308, 440, 777, 860, 873
AlwNI CAGNNNCTG 1 cut(s) 648
Ama87I CYCGRG 2 cut(s) 341, 897
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 775
ApoI RAATTY 3 cut(s) 788, 958, 989
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 2 cut(s) 149, 744
AspS9I GGNCC 3 cut(s) 133, 378, 694
AsuC2I CCSGG 7 cut(s) 342, 343, 713, 827, 898, 899, 1046
AsuHPI GGTGA 3 cut(s) 163, 217, 322
AvaI CYCGRG 2 cut(s) 341, 897
AvaII GGWCC 2 cut(s) 378, 694
BaeGI GKGCMC 1 cut(s) 931
BamHI GGATCC 1 cut(s) 865
BanI GGYRCC 1 cut(s) 928
BarI GAAGNNNNNNTAC 4 cut(s) 590, 599, 622, 631
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 787
BccI CCATC 5 cut(s) 53, 341, 583, 853, 866
BceAI ACGGC 5 cut(s) 134, 419, 452, 729, 784
BciT130I CCWGG 2 cut(s) 131, 667
BcnI CCSGG 7 cut(s) 342, 343, 713, 827, 898, 899, 1046
BcoDI GTCTC 1 cut(s) 650
BisI GCNGC 4 cut(s) 111, 663, 776, 950
BlsI GCNGC 4 cut(s) 112, 664, 777, 951
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 9 cut(s) 131, 342, 343, 667, 713, 827, 898, 899, 1046
Bme18I GGWCC 2 cut(s) 378, 694
BmeT110I CYCGRG 2 cut(s) 341, 897
BmgT120I GGNCC 3 cut(s) 133, 378, 694
BmiI GGNNCC 3 cut(s) 696, 867, 930
BmrFI CCNGG 9 cut(s) 131, 342, 343, 667, 713, 827, 898, 899, 1046
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 1 cut(s) 675
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 451
BpuMI CCSGG 7 cut(s) 342, 343, 713, 827, 898, 899, 1046
BsaBI GATNNNNATC 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 4 cut(s) 341, 342, 666, 897
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 6 cut(s) 322, 352, 374, 404, 803, 833
Bsc4I CCNNNNNNNGG 7 cut(s) 255, 343, 375, 648, 672, 712, 1019
Bse1I ACTGG 3 cut(s) 478, 642, 706
Bse8I GATNNNNATC 1 cut(s) 160
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 2 cut(s) 131, 667
BseDI CCNNGG 4 cut(s) 341, 342, 666, 897
BseGI GGATG 4 cut(s) 45, 333, 594, 877
BseJI GATNNNNATC 1 cut(s) 160
BseLI CCNNNNNNNGG 7 cut(s) 255, 343, 375, 648, 672, 712, 1019
BseNI ACTGG 3 cut(s) 478, 642, 706
BseRI GAGGAG 5 cut(s) 26, 29, 44, 53, 845
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 1 cut(s) 787
Bsh1236I CGCG 3 cut(s) 147, 354, 742
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 1 cut(s) 134
BshNI GGYRCC 1 cut(s) 928
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 2 cut(s) 341, 897
BsiSI CCGG 8 cut(s) 342, 689, 712, 827, 869, 898, 998, 1045
BslFI GGGAC 2 cut(s) 473, 680
BslI CCNNNNNNNGG 7 cut(s) 255, 343, 375, 648, 672, 712, 1019
BsmAI GTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 473, 680
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 1 cut(s) 134
BsoBI CYCGRG 2 cut(s) 341, 897
Bsp1286I GDGCHC 2 cut(s) 441, 931
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
BspANI GGCC 1 cut(s) 134
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 3 cut(s) 147, 354, 742
BspLI GGNNCC 3 cut(s) 696, 867, 930
BspPI GGATC 7 cut(s) 97, 150, 308, 440, 777, 860, 873
BspT107I GGYRCC 1 cut(s) 928
BsrBI CCGCTC 1 cut(s) 519
BsrI ACTGG 3 cut(s) 478, 642, 706
BssECI CCNNGG 4 cut(s) 341, 342, 666, 897
BssMI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
BssNI GRCGYC 1 cut(s) 573
Bst2UI CCWGG 2 cut(s) 131, 667
Bst4CI ACNGT 3 cut(s) 359, 386, 919
Bst6I CTCTTC 4 cut(s) 62, 143, 224, 530
BstACI GRCGYC 1 cut(s) 573
BstC8I GCNNGC 1 cut(s) 426
BstF5I GGATG 4 cut(s) 45, 333, 594, 877
BstFNI CGCG 3 cut(s) 147, 354, 742
BstHHI GCGC 2 cut(s) 149, 744
BstKTI GATC 7 cut(s) 105, 158, 316, 405, 448, 772, 868
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 1 cut(s) 781
BstNI CCWGG 2 cut(s) 131, 667
BstSCI CCNGG 9 cut(s) 129, 340, 341, 665, 711, 825, 896, 897, 1044
BstSLI GKGCMC 1 cut(s) 931
BstUI CGCG 3 cut(s) 147, 354, 742
BstV1I GCAGC 1 cut(s) 787
BstV2I GAAGAC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 865
BstYI RGATCY 1 cut(s) 865
BsuRI GGCC 1 cut(s) 134
BtgZI GCGATG 1 cut(s) 349
BtsCI GGATG 4 cut(s) 45, 333, 594, 877
BtsIMutI CAGTG 1 cut(s) 924
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 648
CfoI GCGC 2 cut(s) 149, 744
Cfr13I GGNCC 3 cut(s) 133, 378, 694
Cfr9I CCCGGG 2 cut(s) 341, 897
CseI GACGC 3 cut(s) 475, 581, 856
Csp6I GTAC 7 cut(s) 20, 481, 607, 658, 676, 879, 915
CviAII CATG 1 cut(s) 682
CviJI RGCY 4 cut(s) 134, 202, 615, 778
CviKI_1 RGCY 4 cut(s) 134, 202, 615, 778
CviQI GTAC 7 cut(s) 20, 481, 607, 658, 676, 879, 915
DpnI GATC 7 cut(s) 104, 157, 315, 404, 447, 771, 867
DpnII GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
DrdI GACNNNNNNGTC 1 cut(s) 853
DseDI GACNNNNNNGTC 1 cut(s) 853
Eam1104I CTCTTC 4 cut(s) 62, 143, 224, 530
EarI CTCTTC 4 cut(s) 62, 143, 224, 530
EciI GGCGGA 2 cut(s) 397, 878
Eco47I GGWCC 2 cut(s) 378, 694
Eco57I CTGAAG 1 cut(s) 785
Eco88I CYCGRG 2 cut(s) 341, 897
EcoO109I RGGNCCY 2 cut(s) 133, 694
EcoRII CCWGG 2 cut(s) 129, 665
FaeI CATG 1 cut(s) 685
FaiI YATR 2 cut(s) 683, 888
FaqI GGGAC 2 cut(s) 473, 680
FatI CATG 1 cut(s) 681
FauI CCCGC 6 cut(s) 115, 152, 422, 457, 560, 565
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 4 cut(s) 111, 663, 776, 950
FokI GGATG 4 cut(s) 32, 320, 601, 884
Fsp4HI GCNGC 4 cut(s) 111, 663, 776, 950
GlaI GCGC 2 cut(s) 148, 743
GluI GCNGC 4 cut(s) 111, 663, 776, 950
HaeIII GGCC 1 cut(s) 134
HapII CCGG 8 cut(s) 342, 689, 712, 827, 869, 898, 998, 1045
HgaI GACGC 3 cut(s) 475, 581, 856
HhaI GCGC 2 cut(s) 149, 744
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 1 cut(s) 685
Hin6I GCGC 2 cut(s) 147, 742
HinP1I GCGC 2 cut(s) 147, 742
HincII GTYRAC 2 cut(s) 325, 582
HindII GTYRAC 2 cut(s) 325, 582
HinfI GANTC 3 cut(s) 188, 583, 968
HpaII CCGG 8 cut(s) 342, 689, 712, 827, 869, 898, 998, 1045
HphI GGTGA 3 cut(s) 163, 217, 322
Hpy166II GTNNAC 5 cut(s) 307, 325, 514, 582, 879
Hpy188I TCNGA 9 cut(s) 155, 235, 571, 588, 765, 846, 894, 967, 1030
Hpy188III TCNNGA 4 cut(s) 100, 649, 809, 869
Hpy8I GTNNAC 5 cut(s) 307, 325, 514, 582, 879
Hpy99I CGWCG 4 cut(s) 128, 446, 575, 850
HpyAV CCTTC 6 cut(s) 37, 223, 511, 666, 760, 1027
HpyCH4III ACNGT 3 cut(s) 359, 386, 919
HpyCH4IV ACGT 3 cut(s) 303, 327, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 781
HpySE526I ACGT 3 cut(s) 303, 327, 510
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 1 cut(s) 685
HspAI GCGC 2 cut(s) 147, 742
Kpn2I TCCGGA 1 cut(s) 868
Kzo9I GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
LmnI GCTCC 2 cut(s) 524, 832
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 1 cut(s) 675
MaeII ACGT 3 cut(s) 303, 327, 510
MaeIII GTNAC 3 cut(s) 328, 746, 821
MalI GATC 7 cut(s) 104, 157, 315, 404, 447, 771, 867
MbiI CCGCTC 1 cut(s) 519
MboI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
MboII GAAGA 7 cut(s) 49, 130, 211, 451, 454, 517, 721
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 2 cut(s) 441, 931
MluCI AATT 5 cut(s) 788, 794, 904, 958, 989
MlyI GAGTC 2 cut(s) 577, 977
MmeI TCCRAC 3 cut(s) 258, 594, 869
MroI TCCGGA 1 cut(s) 868
MseI TTAA 1 cut(s) 903
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 778
MspI CCGG 8 cut(s) 342, 689, 712, 827, 869, 898, 998, 1045
MspR9I CCNGG 9 cut(s) 131, 342, 343, 667, 713, 827, 898, 899, 1046
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 2 cut(s) 131, 667
MvnI CGCG 3 cut(s) 147, 354, 742
MwoI GCNNNNNNNGC 1 cut(s) 781
NciI CCSGG 7 cut(s) 342, 343, 713, 827, 898, 899, 1046
NdeII GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
NlaIII CATG 1 cut(s) 685
NlaIV GGNNCC 3 cut(s) 696, 867, 930
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 2 cut(s) 328, 746
OliI CACNNNNGTG 1 cut(s) 434
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 432, 854
PctI GAATGC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 648
PfoI TCCNGGA 1 cut(s) 1044
PkrI GCNGC 4 cut(s) 112, 664, 777, 951
PleI GAGTC 2 cut(s) 577, 976
PpsI GAGTC 2 cut(s) 577, 976
PpuMI RGGWCCY 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 2 cut(s) 129, 665
PspGI CCWGG 2 cut(s) 129, 665
PspN4I GGNNCC 3 cut(s) 696, 867, 930
PspPI GGNCC 3 cut(s) 133, 378, 694
PspPPI RGGWCCY 1 cut(s) 694
PstNI CAGNNNCTG 1 cut(s) 648
PsuI RGATCY 1 cut(s) 865
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 7 cut(s) 21, 482, 608, 659, 677, 880, 916
RsaNI GTAC 7 cut(s) 20, 481, 607, 658, 676, 879, 915
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 903
SatI GCNGC 4 cut(s) 111, 663, 776, 950
Sau3AI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
Sau96I GGNCC 3 cut(s) 133, 378, 694
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 2 cut(s) 577, 977
ScrFI CCNGG 9 cut(s) 131, 342, 343, 667, 713, 827, 898, 899, 1046
SduI GDGCHC 2 cut(s) 441, 931
SfaNI GCATC 1 cut(s) 675
SinI GGWCC 2 cut(s) 378, 694
SmaI CCCGGG 2 cut(s) 343, 899
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 5 cut(s) 788, 794, 904, 958, 989
SspI AATATT 1 cut(s) 298
StyD4I CCNGG 9 cut(s) 129, 340, 341, 665, 711, 825, 896, 897, 1044
TaaI ACNGT 3 cut(s) 359, 386, 919
TaiI ACGT 3 cut(s) 306, 330, 513
TaqI TCGA 7 cut(s) 123, 186, 441, 543, 621, 638, 857
TasI AATT 5 cut(s) 788, 794, 904, 958, 989
TatI WGTACW 1 cut(s) 480
TauI GCSGC 3 cut(s) 113, 665, 952
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 1 cut(s) 903
Tru9I TTAA 1 cut(s) 903
TscAI CASTG 1 cut(s) 924
TseFI GTSAC 2 cut(s) 328, 746
TseI GCWGC 1 cut(s) 775
Tsp45I GTSAC 2 cut(s) 328, 746
TspDTI ATGAA 3 cut(s) 132, 352, 607
TspGWI ACGGA 1 cut(s) 310
TspMI CCCGGG 2 cut(s) 341, 897
TspRI CASTG 1 cut(s) 924
Van91I CCANNNNNTGG 1 cut(s) 648
VpaK11BI GGWCC 2 cut(s) 378, 694
XapI RAATTY 3 cut(s) 788, 958, 989
XmaI CCCGGG 2 cut(s) 341, 897
XmiI GTMKAC 1 cut(s) 513
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.