Prupe.6G146600_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0016215
Physical Location & Seq
Reverse (-)
12140814 .. 12142841
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G146600.1

Sequence Viewer

Length: 1101 bp
ATGTTCTCCTCCACCCCATTCCTCGTCTCCTTCCTCCTCCTCCTCTCCCTCTCTCTCCTCTTCCTCTTCGCCCCACGCTTCCTCCCCCCTTCCCGCCCTCCGATCCCCATATCCGCCTCCGACGAGCTCGACGACCAGATCCTCTTCAACCGCGCCCTCAACCCTAACCCTAGAAAACCCAAGCCTACATTCTCCCACCTCGCCCTCGATTCGAAATCCTCCAAACCCAAGATCGCCTTCCTCTTCCTCACCAACTCTGACCTCCACTTCGCCCCCCTCTGGTCCCTTTTCTTCTCCAAAACCAACTCCAATCTTTACAATATTTACGTCCATGCCGATCCCGCCGCCAACGTCACCCTCCCTCCCGGCACCGTCTTCCACAACCGCCTCGTCCCTTCCAAGCGCACTTACCGCGCCTCCGCAACCCTCATCTCCGCCACGCGCCGCCTCCTTGCCTCTGCTGTCATCGACGACCCTGCCAATCTCTTCTTCGCCGTTTTATCCCAGTACTGCGTACCCCTCCACTCCTTCCGGTACGTCTACAACTCGCTCTTTTTCTCCACCACCTTCGACTTGACCCGACCCGCCACCGGCTCCGACGCCGAGTTGGCCCAGATGGGCCTCAAGGTCCGGCCCAAGAGCTTCATCGAGATCCTCTCCAAATCGGCCAGTCTCTGGAAGCGGTACTCCGCCCGTGGACGCTTCGCGATGATGCCGGAGGTCCCGTTCGAGCAGTTCCGGTTCTTTCTCCTCACCCGACGCCACGCGCTCGTGGTTTTGAAGGATCGGGCGCTGTGGAGAAAATTCAGAATGCCGTGCTATCGAGAAGACCAGTGCTACCCTGAGGAGCACTATTTTCCGACGTTGTTGTCAATGGCGGATCCGGATGGGCTAACCCGTTACAGCCTGACCCGGGTAAATTGGACGGGTACGGTAAAAGGGCACCCGTACACTTACCTAGCCGGGGAAGTGTCGGCGGAGCTGATTCATCGGCTGCGGAAATCGAATTTGTCGGAGTCGTACCTGTTCGCGAGGAAGTTCTCGCCGGATTGCTTGAAACCCTTGTTGGGTTTGGCCGAGAAGGTCATTTTCCGGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

367

Amino Acids

41.9

Weight (kDa)

9.85

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22070
fragaria_vesca FvH4_2g09440
malus_domestica MD03G1158600.v1.1
prunus_persica Prupe.6G146600_v2.0.a1 Prupe.6G146600_v2.0.a1
pyrus_communis pycom03g11090
rosa_chinensis RchiOBHm_Chr5g0048971
rosa_laevigata RLG00000034575
rosa_multiflora Rmu_sc0004058.1_g000017
rosa_rugosa Rorug05G0241600
rosa_samantha Rh5AG321800 Rh5BG332000 Rh5CG357300 Rh5DG343800
rosa_wichuraiana Rw5G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 868
AccB1I GGYRCC 2 cut(s) 368, 942
AccB7I CCANNNNNTGG 1 cut(s) 675
AccI GTMKAC 1 cut(s) 540
AccII CGCG 6 cut(s) 153, 414, 442, 707, 767, 1031
AccIII TCCGGA 1 cut(s) 883
AclWI GGATC 7 cut(s) 97, 133, 332, 646, 792, 875, 888
AcoI YGGCCR 2 cut(s) 666, 1074
AcsI RAATTY 2 cut(s) 803, 1006
AcyI GRCGYC 2 cut(s) 600, 760
AfaI GTAC 7 cut(s) 509, 516, 536, 686, 931, 950, 1022
AfiI CCNNNNNNNGG 6 cut(s) 279, 590, 675, 913, 964, 1067
AgsI TTSAA 3 cut(s) 148, 781, 1057
AjuI GAANNNNNNNTTGG 8 cut(s) 173, 205, 221, 253, 473, 505, 1049, 1081
AloI GAACNNNNNNTCC 2 cut(s) 710, 742
AluBI AGCT 3 cut(s) 127, 642, 982
AluI AGCT 3 cut(s) 127, 642, 982
Alw21I GWGCWC 2 cut(s) 129, 852
Alw26I GTCTC 2 cut(s) 31, 677
AlwI GGATC 7 cut(s) 97, 133, 332, 646, 792, 875, 888
AlwNI CAGNNNCTG 1 cut(s) 675
Ama87I CYCGRG 1 cut(s) 912
Aor13HI TCCGGA 1 cut(s) 883
AoxI GGCC 5 cut(s) 609, 619, 632, 666, 1074
ApeKI GCWGC 1 cut(s) 994
ApoI RAATTY 2 cut(s) 803, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AspLEI GCGC 6 cut(s) 155, 405, 416, 444, 769, 793
AspS9I GGNCC 6 cut(s) 282, 610, 619, 628, 633, 721
AsuC2I CCSGG 5 cut(s) 366, 913, 914, 964, 1094
AsuHPI GGTGA 3 cut(s) 241, 346, 745
AsuII TTCGAA 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 912
AvaII GGWCC 3 cut(s) 282, 628, 721
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 1 cut(s) 945
BamHI GGATCC 1 cut(s) 880
BanI GGYRCC 2 cut(s) 368, 942
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 1 cut(s) 770
BbsI GAAGAC 2 cut(s) 367, 834
Bbv12I GWGCWC 2 cut(s) 129, 852
BbvI GCAGC 1 cut(s) 981
BccI CCATC 2 cut(s) 610, 881
BceAI ACGGC 2 cut(s) 479, 799
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BcnI CCSGG 5 cut(s) 366, 913, 914, 964, 1094
BcoDI GTCTC 2 cut(s) 31, 677
BfaI CTAG 2 cut(s) 171, 959
BfoI RGCGCY 1 cut(s) 794
BglI GCCNNNNNGGC 1 cut(s) 608
BisI GCNGC 3 cut(s) 345, 445, 995
BlsI GCNGC 3 cut(s) 346, 446, 996
BmcAI AGTACT 1 cut(s) 509
Bme1390I CCNGG 5 cut(s) 366, 913, 914, 964, 1094
Bme18I GGWCC 3 cut(s) 282, 628, 721
BmeT110I CYCGRG 1 cut(s) 912
BmgT120I GGNCC 6 cut(s) 282, 610, 619, 628, 633, 721
BmiI GGNNCC 6 cut(s) 284, 370, 595, 723, 882, 944
BmrFI CCNGG 5 cut(s) 366, 913, 914, 964, 1094
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 2 cut(s) 367, 834
BplI GAGNNNNNCTC 2 cut(s) 641, 673
Bpu14I TTCGAA 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 5 cut(s) 366, 913, 914, 964, 1094
BsaHI GRCGYC 2 cut(s) 600, 760
BsaJI CCNNGG 3 cut(s) 694, 912, 963
BsaWI WCCGGW 3 cut(s) 531, 738, 883
BsaXI ACNNNNNCTCC 8 cut(s) 66, 96, 346, 376, 401, 431, 710, 740
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 590, 675, 913, 964, 1067
Bse118I RCCGGY 1 cut(s) 590
Bse1I ACTGG 3 cut(s) 505, 669, 832
Bse21I CCTNAGG 1 cut(s) 843
BseAI TCCGGA 1 cut(s) 883
BseDI CCNNGG 3 cut(s) 694, 912, 963
BseGI GGATG 1 cut(s) 892
BseLI CCNNNNNNNGG 6 cut(s) 279, 590, 675, 913, 964, 1067
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 3 cut(s) 505, 669, 832
BseRI GAGGAG 6 cut(s) 26, 29, 32, 47, 740, 860
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 1 cut(s) 981
Bsh1236I CGCG 6 cut(s) 153, 414, 442, 707, 767, 1031
BshFI GGCC 5 cut(s) 611, 621, 634, 668, 1076
BshNI GGYRCC 2 cut(s) 368, 942
BsiHKAI GWGCWC 2 cut(s) 129, 852
BsiHKCI CYCGRG 1 cut(s) 912
BslFI GGGAC 3 cut(s) 268, 377, 707
BslI CCNNNNNNNGG 6 cut(s) 279, 590, 675, 913, 964, 1067
BsmAI GTCTC 2 cut(s) 31, 677
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 3 cut(s) 268, 377, 707
BsmI GAATGC 1 cut(s) 816
BsnI GGCC 5 cut(s) 611, 621, 634, 668, 1076
BsoBI CYCGRG 1 cut(s) 912
Bsp119I TTCGAA 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 129, 852, 945
Bsp13I TCCGGA 1 cut(s) 883
Bsp143I GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
Bsp68I TCGCGA 2 cut(s) 707, 1031
BspANI GGCC 5 cut(s) 611, 621, 634, 668, 1076
BspCNI CTCAG 1 cut(s) 835
BspEI TCCGGA 1 cut(s) 883
BspFNI CGCG 6 cut(s) 153, 414, 442, 707, 767, 1031
BspLI GGNNCC 6 cut(s) 284, 370, 595, 723, 882, 944
BspPI GGATC 7 cut(s) 97, 133, 332, 646, 792, 875, 888
BspT104I TTCGAA 1 cut(s) 212
BspT107I GGYRCC 2 cut(s) 368, 942
BsrFI RCCGGY 1 cut(s) 590
BsrI ACTGG 3 cut(s) 505, 669, 832
BssAI RCCGGY 1 cut(s) 590
BssECI CCNNGG 3 cut(s) 694, 912, 963
BssMI GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
BssNI GRCGYC 2 cut(s) 600, 760
BssSI CACGAG 1 cut(s) 770
Bst2BI CACGAG 1 cut(s) 770
Bst4CI ACNGT 2 cut(s) 373, 934
Bst6I CTCTTC 5 cut(s) 65, 71, 149, 248, 491
BstACI GRCGYC 2 cut(s) 600, 760
BstBI TTCGAA 1 cut(s) 212
BstDEI CTNAG 1 cut(s) 843
BstDSI CCRYGG 1 cut(s) 694
BstF5I GGATG 1 cut(s) 892
BstFNI CGCG 6 cut(s) 153, 414, 442, 707, 767, 1031
BstH2I RGCGCY 1 cut(s) 794
BstHHI GCGC 6 cut(s) 155, 405, 416, 444, 769, 793
BstKTI GATC 7 cut(s) 105, 141, 234, 340, 654, 787, 883
BstMAI GTCTC 2 cut(s) 31, 677
BstMBI GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
BstMWI GCNNNNNNNGC 3 cut(s) 341, 411, 608
BstSCI CCNGG 5 cut(s) 364, 911, 912, 962, 1092
BstSLI GKGCMC 1 cut(s) 945
BstUI CGCG 6 cut(s) 153, 414, 442, 707, 767, 1031
BstV1I GCAGC 1 cut(s) 981
BstV2I GAAGAC 2 cut(s) 367, 834
BstX2I RGATCY 3 cut(s) 138, 651, 880
BstYI RGATCY 3 cut(s) 138, 651, 880
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 5 cut(s) 611, 621, 634, 668, 1076
BtgI CCRYGG 1 cut(s) 694
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 1 cut(s) 892
BtsIMutI CAGTG 1 cut(s) 839
BtuMI TCGCGA 2 cut(s) 707, 1031
CaiI CAGNNNCTG 1 cut(s) 675
CfoI GCGC 6 cut(s) 155, 405, 416, 444, 769, 793
Cfr10I RCCGGY 1 cut(s) 590
Cfr13I GGNCC 6 cut(s) 282, 610, 619, 628, 633, 721
Cfr9I CCCGGG 1 cut(s) 912
CseI GACGC 3 cut(s) 608, 708, 768
Csp6I GTAC 7 cut(s) 508, 515, 535, 685, 930, 949, 1021
CviAII CATG 1 cut(s) 332
CviQI GTAC 7 cut(s) 508, 515, 535, 685, 930, 949, 1021
DdeI CTNAG 1 cut(s) 843
DpnI GATC 7 cut(s) 104, 140, 233, 339, 653, 786, 882
DpnII GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
DrdI GACNNNNNNGTC 1 cut(s) 868
DseDI GACNNNNNNGTC 1 cut(s) 868
EaeI YGGCCR 2 cut(s) 666, 1074
Eam1104I CTCTTC 5 cut(s) 65, 71, 149, 248, 491
EarI CTCTTC 5 cut(s) 65, 71, 149, 248, 491
EciI GGCGGA 5 cut(s) 103, 424, 679, 893, 992
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 3 cut(s) 282, 628, 721
Eco53kI GAGCTC 1 cut(s) 127
Eco81I CCTNAGG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 912
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 721
EcoT38I GRGCYC 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 1 cut(s) 335
FaiI YATR 2 cut(s) 110, 333
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 3 cut(s) 268, 377, 707
FatI CATG 1 cut(s) 331
FauI CCCGC 3 cut(s) 101, 349, 592
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 3 cut(s) 345, 445, 995
FokI GGATG 1 cut(s) 899
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 3 cut(s) 345, 445, 995
FspBI CTAG 2 cut(s) 171, 959
GlaI GCGC 6 cut(s) 154, 404, 415, 443, 768, 792
GluI GCNGC 3 cut(s) 345, 445, 995
HaeII RGCGCY 1 cut(s) 794
HaeIII GGCC 5 cut(s) 611, 621, 634, 668, 1076
HgaI GACGC 3 cut(s) 608, 708, 768
HhaI GCGC 6 cut(s) 155, 405, 416, 444, 769, 793
Hin1I GRCGYC 2 cut(s) 600, 760
Hin1II CATG 1 cut(s) 335
Hin6I GCGC 6 cut(s) 153, 403, 414, 442, 767, 791
HinP1I GCGC 6 cut(s) 153, 403, 414, 442, 767, 791
HinfI GANTC 3 cut(s) 209, 985, 1016
HphI GGTGA 3 cut(s) 241, 346, 745
Hpy166II GTNNAC 3 cut(s) 541, 698, 951
Hpy188I TCNGA 7 cut(s) 102, 121, 259, 598, 809, 861, 1015
Hpy188III TCNNGA 6 cut(s) 649, 676, 706, 824, 884, 1030
Hpy8I GTNNAC 3 cut(s) 541, 698, 951
Hpy99I CGWCG 6 cut(s) 125, 134, 473, 602, 762, 865
HpyAV CCTTC 8 cut(s) 40, 99, 247, 405, 538, 577, 775, 1075
HpyCH4III ACNGT 2 cut(s) 373, 934
HpyCH4IV ACGT 4 cut(s) 327, 351, 537, 863
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 411, 608
HpyF3I CTNAG 1 cut(s) 843
HpySE526I ACGT 4 cut(s) 327, 351, 537, 863
Hsp92I GRCGYC 2 cut(s) 600, 760
Hsp92II CATG 1 cut(s) 335
HspAI GCGC 6 cut(s) 153, 403, 414, 442, 767, 791
Kpn2I TCCGGA 1 cut(s) 883
Kzo9I GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
LmnI GCTCC 3 cut(s) 599, 847, 979
Lsp1109I GCAGC 1 cut(s) 981
LweI GCATC 1 cut(s) 702
MaeI CTAG 2 cut(s) 171, 959
MaeII ACGT 4 cut(s) 327, 351, 537, 863
MaeIII GTNAC 2 cut(s) 352, 899
MalI GATC 7 cut(s) 104, 140, 233, 339, 653, 786, 882
MboI GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
MboII GAAGA 9 cut(s) 52, 58, 136, 235, 283, 367, 478, 481, 839
MflI RGATCY 3 cut(s) 138, 651, 880
MhlI GDGCHC 3 cut(s) 129, 852, 945
MluCI AATT 3 cut(s) 803, 919, 1006
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 4 cut(s) 144, 621, 884, 993
MroI TCCGGA 1 cut(s) 883
MspR9I CCNGG 5 cut(s) 366, 913, 914, 964, 1094
Mva1269I GAATGC 1 cut(s) 816
MvnI CGCG 6 cut(s) 153, 414, 442, 707, 767, 1031
MwoI GCNNNNNNNGC 3 cut(s) 341, 411, 608
NciI CCSGG 5 cut(s) 366, 913, 914, 964, 1094
NdeII GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
NlaIII CATG 1 cut(s) 335
NlaIV GGNNCC 6 cut(s) 284, 370, 595, 723, 882, 944
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 1 cut(s) 352
NruI TCGCGA 2 cut(s) 707, 1031
NspV TTCGAA 1 cut(s) 212
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 333
PctI GAATGC 1 cut(s) 816
PfeI GAWTC 2 cut(s) 209, 985
PflMI CCANNNNNTGG 1 cut(s) 675
PfoI TCCNGGA 1 cut(s) 1092
PkrI GCNGC 3 cut(s) 346, 446, 996
PleI GAGTC 1 cut(s) 1024
PpsI GAGTC 1 cut(s) 1024
PpuMI RGGWCCY 1 cut(s) 721
Psp124BI GAGCTC 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 721
PspN4I GGNNCC 6 cut(s) 284, 370, 595, 723, 882, 944
PspPI GGNCC 6 cut(s) 282, 610, 619, 628, 633, 721
PspPPI RGGWCCY 1 cut(s) 721
PstNI CAGNNNCTG 1 cut(s) 675
PsuI RGATCY 3 cut(s) 138, 651, 880
RruI TCGCGA 2 cut(s) 707, 1031
RsaI GTAC 7 cut(s) 509, 516, 536, 686, 931, 950, 1022
RsaNI GTAC 7 cut(s) 508, 515, 535, 685, 930, 949, 1021
SacI GAGCTC 1 cut(s) 129
SatI GCNGC 3 cut(s) 345, 445, 995
Sau3AI GATC 7 cut(s) 102, 138, 231, 337, 651, 784, 880
Sau96I GGNCC 6 cut(s) 282, 610, 619, 628, 633, 721
ScaI AGTACT 1 cut(s) 509
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 5 cut(s) 366, 913, 914, 964, 1094
SduI GDGCHC 3 cut(s) 129, 852, 945
SfaNI GCATC 1 cut(s) 702
SfuI TTCGAA 1 cut(s) 212
SinI GGWCC 3 cut(s) 282, 628, 721
SmaI CCCGGG 1 cut(s) 914
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 3 cut(s) 803, 919, 1006
SspI AATATT 1 cut(s) 322
SspMI CTAG 2 cut(s) 171, 959
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 5 cut(s) 364, 911, 912, 962, 1092
TaaI ACNGT 2 cut(s) 373, 934
TaiI ACGT 4 cut(s) 330, 354, 540, 866
TaqI TCGA 9 cut(s) 129, 207, 212, 468, 570, 648, 729, 823, 1004
TasI AATT 3 cut(s) 803, 919, 1006
TatI WGTACW 1 cut(s) 507
TauI GCSGC 2 cut(s) 347, 447
TfiI GAWTC 2 cut(s) 209, 985
TscAI CASTG 1 cut(s) 839
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 994
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 634, 977
TspMI CCCGGG 1 cut(s) 912
TspRI CASTG 1 cut(s) 839
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 3 cut(s) 282, 628, 721
XapI RAATTY 2 cut(s) 803, 1006
XmaI CCCGGG 1 cut(s) 912
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 2 cut(s) 171, 959
ZrmI AGTACT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.