RLG00000034575

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016215
Physical Location & Seq
Reverse (-)
45199348 .. 45201088
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034575

Sequence Viewer

Length: 1062 bp
ATGTTCTCCACCCCATTCGTACTCTCCTTCGCCCTCCTCCTCTCCATCCCTCTCCTCTTCCTCCTCGCCCCCCATTTCCTCCCTCCCAACAACCAAATCCCGATCCCCGCCGCCGATGAAATCGACGACCAGGCCCTCTTCTCCCGCGCCGTCCGATCCAAATCCGCATTCACCCGCCTCTCCCTCGATTCCAAAACCAAGCCCAAAATCGCCTTCCTCTTCCTCACCAACTCCGACCTCCACTTCGCCCCCCTCTGGCAACGCTTCTTTTCCAAAACTGCCCCCAATCTCTACAATATTTACGTCCACGCCGATCCCTCCGCCAACGTCACCATCCCTCCGGGGGTTTTCCGCGACCGTTTCATCGCCTCTAAGCGCACTTACCGTGCCTCCCCGACCCTGATCTCCGCCACCCGCCGATTGCTCGCCACCGCCGTGCTCGACGATCCGGCCAATGTCTTCTTCGCCGTTCTCTCCCAGTACTGCGTCCCTCTCCATTCCTTCCGCTACGTCTACCGCTCCCTCTTCGCCTCCGCCACTTTCGACAAGTCCCGGCCCGCCACCGAGTCCGACGCCGAGTTGACTCGGATGGGAGTGAAGGTCAGGTACAAGAGCTTCATCGAAATCCTCTCCAAATCGACCAGTCTCTGGAAGCGGTACGCCGCCAGGGGAAGGTACGCCATGATGCCGGAGGTCCCATTCGACCAGTTCCGGGTCGGGTCGCAGTTCTTCTTGCTCACTCGCCGTCACGCATTGGTGGTTTTGAAGGATCGGCAGCTGTGGCAGAAATTCAAATTGCCGTGTTATCGGGAGGACGAGTGTTACCCGGAGGAGCATTACTTTCCGACGCTGTTGTCGATGGCGGATCCGGATGGGTGTACTCATTATACTCTGACCCGGGTTAATTGGACGGTCAAATTTTTCCGAGTCTTACTTGTTTGCGAGGAAGTTCTCGCCGGATTGCTTGAAACCCTTGTTGGGTTTATCCGAGAAGGTCATTTTCCGGGACTGAGGTTCTTCCCATTTACCCTGGTAAAAAAAAGGCCAGTGAAGATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

354

Amino Acids

40.68

Weight (kDa)

9.77

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 70 - 304 2.9e-50 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22070
fragaria_vesca FvH4_2g09440
malus_domestica MD03G1158600.v1.1
prunus_persica Prupe.6G146600_v2.0.a1 Prupe.6G146600_v2.0.a1
pyrus_communis pycom03g11090
rosa_chinensis RchiOBHm_Chr5g0048971
rosa_laevigata RLG00000034575
rosa_multiflora Rmu_sc0004058.1_g000017
rosa_rugosa Rorug05G0241600
rosa_samantha Rh5AG321800 Rh5BG332000 Rh5CG357300 Rh5DG343800
rosa_wichuraiana Rw5G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 853
AccB7I CCANNNNNTGG 1 cut(s) 648
AccBSI CCGCTC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 513
AccII CGCG 2 cut(s) 147, 354
AccIII TCCGGA 1 cut(s) 868
AclWI GGATC 7 cut(s) 97, 150, 308, 440, 777, 860, 873
AcoI YGGCCR 1 cut(s) 450
AcsI RAATTY 2 cut(s) 788, 917
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 6 cut(s) 21, 482, 608, 659, 677, 880
AfiI CCNNNNNNNGG 6 cut(s) 255, 343, 648, 672, 712, 978
AgsI TTSAA 3 cut(s) 766, 793, 968
AjnI CCWGG 3 cut(s) 129, 665, 1029
AjuI GAANNNNNNNTTGG 8 cut(s) 152, 184, 197, 229, 446, 478, 960, 992
AleI CACNNNNGTG 1 cut(s) 434
AluBI AGCT 2 cut(s) 615, 778
AluI AGCT 2 cut(s) 615, 778
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 7 cut(s) 97, 150, 308, 440, 777, 860, 873
AlwNI CAGNNNCTG 1 cut(s) 648
Ama87I CYCGRG 1 cut(s) 897
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 4 cut(s) 132, 450, 554, 1043
ApeKI GCWGC 1 cut(s) 775
ApoI RAATTY 2 cut(s) 788, 917
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 2 cut(s) 149, 378
AspS9I GGNCC 3 cut(s) 133, 555, 694
AsuC2I CCSGG 7 cut(s) 342, 553, 713, 827, 898, 899, 1005
AsuHPI GGTGA 3 cut(s) 163, 217, 322
AvaI CYCGRG 1 cut(s) 897
AvaII GGWCC 1 cut(s) 694
BamHI GGATCC 1 cut(s) 865
BarI GAAGNNNNNNTAC 4 cut(s) 590, 599, 622, 631
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 787
BccI CCATC 5 cut(s) 53, 341, 583, 853, 866
BceAI ACGGC 5 cut(s) 134, 419, 452, 729, 784
BciT130I CCWGG 3 cut(s) 131, 667, 1031
BcnI CCSGG 7 cut(s) 342, 553, 713, 827, 898, 899, 1005
BcoDI GTCTC 1 cut(s) 650
BisI GCNGC 3 cut(s) 111, 663, 776
BlsI GCNGC 3 cut(s) 112, 664, 777
BmcAI AGTACT 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 694
BmeT110I CYCGRG 1 cut(s) 897
BmgT120I GGNCC 3 cut(s) 133, 555, 694
BmiI GGNNCC 2 cut(s) 696, 867
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 1 cut(s) 675
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 451
BpuMI CCSGG 7 cut(s) 342, 553, 713, 827, 898, 899, 1005
BsaBI GATNNNNATC 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 4 cut(s) 341, 666, 897, 1029
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 8 cut(s) 322, 352, 374, 404, 585, 615, 803, 833
Bsc4I CCNNNNNNNGG 6 cut(s) 255, 343, 648, 672, 712, 978
Bse1I ACTGG 4 cut(s) 478, 642, 706, 1046
Bse8I GATNNNNATC 1 cut(s) 160
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 3 cut(s) 131, 667, 1031
BseDI CCNNGG 4 cut(s) 341, 666, 897, 1029
BseGI GGATG 4 cut(s) 45, 333, 594, 877
BseJI GATNNNNATC 1 cut(s) 160
BseLI CCNNNNNNNGG 6 cut(s) 255, 343, 648, 672, 712, 978
BseMII CTCAG 1 cut(s) 1001
BseNI ACTGG 4 cut(s) 478, 642, 706, 1046
BseRI GAGGAG 5 cut(s) 26, 29, 44, 53, 845
BseXI GCAGC 1 cut(s) 787
Bsh1236I CGCG 2 cut(s) 147, 354
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 4 cut(s) 134, 452, 556, 1045
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 897
BslFI GGGAC 4 cut(s) 473, 535, 680, 1020
BslI CCNNNNNNNGG 6 cut(s) 255, 343, 648, 672, 712, 978
BsmAI GTCTC 1 cut(s) 650
BsmFI GGGAC 4 cut(s) 473, 535, 680, 1020
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 4 cut(s) 134, 452, 556, 1045
BsoBI CYCGRG 1 cut(s) 897
Bsp1286I GDGCHC 1 cut(s) 441
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
BspANI GGCC 4 cut(s) 134, 452, 556, 1045
BspCNI CTCAG 1 cut(s) 1002
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 2 cut(s) 147, 354
BspLI GGNNCC 2 cut(s) 696, 867
BspPI GGATC 7 cut(s) 97, 150, 308, 440, 777, 860, 873
BsrBI CCGCTC 1 cut(s) 519
BsrI ACTGG 4 cut(s) 478, 642, 706, 1046
BssECI CCNNGG 4 cut(s) 341, 666, 897, 1029
BssMI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
BssNI GRCGYC 1 cut(s) 573
Bst2UI CCWGG 3 cut(s) 131, 667, 1031
Bst4CI ACNGT 3 cut(s) 359, 386, 913
Bst6I CTCTTC 4 cut(s) 62, 143, 224, 530
BstACI GRCGYC 1 cut(s) 573
BstC8I GCNNGC 2 cut(s) 426, 558
BstDEI CTNAG 2 cut(s) 372, 1010
BstF5I GGATG 4 cut(s) 45, 333, 594, 877
BstFNI CGCG 2 cut(s) 147, 354
BstHHI GCGC 2 cut(s) 149, 378
BstKTI GATC 7 cut(s) 105, 158, 316, 405, 448, 772, 868
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 1 cut(s) 781
BstNI CCWGG 3 cut(s) 131, 667, 1031
BstUI CGCG 2 cut(s) 147, 354
BstV1I GCAGC 1 cut(s) 787
BstV2I GAAGAC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 865
BstYI RGATCY 1 cut(s) 865
BsuRI GGCC 4 cut(s) 134, 452, 556, 1045
BtgZI GCGATG 1 cut(s) 349
BtsCI GGATG 4 cut(s) 45, 333, 594, 877
BtsIMutI CAGTG 1 cut(s) 1053
Cac8I GCNNGC 2 cut(s) 426, 558
CaiI CAGNNNCTG 1 cut(s) 648
CfoI GCGC 2 cut(s) 149, 378
Cfr13I GGNCC 3 cut(s) 133, 555, 694
Cfr9I CCCGGG 1 cut(s) 897
CseI GACGC 3 cut(s) 475, 581, 856
Csp6I GTAC 6 cut(s) 20, 481, 607, 658, 676, 879
CviAII CATG 1 cut(s) 682
CviJI RGCY 7 cut(s) 134, 202, 452, 556, 615, 778, 1045
CviKI_1 RGCY 7 cut(s) 134, 202, 452, 556, 615, 778, 1045
CviQI GTAC 6 cut(s) 20, 481, 607, 658, 676, 879
DdeI CTNAG 2 cut(s) 372, 1010
DpnI GATC 7 cut(s) 104, 157, 315, 404, 447, 771, 867
DpnII GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
DrdI GACNNNNNNGTC 1 cut(s) 853
DseDI GACNNNNNNGTC 1 cut(s) 853
EaeI YGGCCR 1 cut(s) 450
Eam1104I CTCTTC 4 cut(s) 62, 143, 224, 530
EarI CTCTTC 4 cut(s) 62, 143, 224, 530
EciI GGCGGA 4 cut(s) 310, 397, 523, 878
Eco47I GGWCC 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 897
EcoO109I RGGNCCY 2 cut(s) 133, 694
EcoRII CCWGG 3 cut(s) 129, 665, 1029
FaeI CATG 1 cut(s) 685
FaiI YATR 2 cut(s) 683, 888
FaqI GGGAC 4 cut(s) 473, 535, 680, 1020
FatI CATG 1 cut(s) 681
FauI CCCGC 5 cut(s) 115, 152, 182, 422, 565
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 3 cut(s) 111, 663, 776
FokI GGATG 4 cut(s) 32, 320, 601, 884
Fsp4HI GCNGC 3 cut(s) 111, 663, 776
GlaI GCGC 2 cut(s) 148, 377
GluI GCNGC 3 cut(s) 111, 663, 776
HaeIII GGCC 4 cut(s) 134, 452, 556, 1045
HgaI GACGC 3 cut(s) 475, 581, 856
HhaI GCGC 2 cut(s) 149, 378
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 1 cut(s) 685
Hin6I GCGC 2 cut(s) 147, 376
HinP1I GCGC 2 cut(s) 147, 376
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HinfI GANTC 4 cut(s) 188, 566, 583, 927
HphI GGTGA 3 cut(s) 163, 217, 322
Hpy166II GTNNAC 4 cut(s) 307, 514, 582, 879
Hpy188I TCNGA 8 cut(s) 155, 235, 571, 588, 846, 894, 926, 989
Hpy188III TCNNGA 4 cut(s) 100, 649, 809, 869
Hpy8I GTNNAC 4 cut(s) 307, 514, 582, 879
Hpy99I CGWCG 4 cut(s) 128, 446, 575, 850
HpyAV CCTTC 7 cut(s) 37, 223, 511, 592, 666, 760, 986
HpyCH4III ACNGT 3 cut(s) 359, 386, 913
HpyCH4IV ACGT 3 cut(s) 303, 327, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 781
HpyF3I CTNAG 2 cut(s) 372, 1010
HpySE526I ACGT 3 cut(s) 303, 327, 510
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 1 cut(s) 685
HspAI GCGC 2 cut(s) 147, 376
Kpn2I TCCGGA 1 cut(s) 868
Kzo9I GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
LmnI GCTCC 2 cut(s) 524, 832
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 1 cut(s) 675
MaeII ACGT 3 cut(s) 303, 327, 510
MaeIII GTNAC 3 cut(s) 328, 746, 821
MalI GATC 7 cut(s) 104, 157, 315, 404, 447, 771, 867
MbiI CCGCTC 1 cut(s) 519
MboI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
MboII GAAGA 8 cut(s) 49, 130, 211, 451, 454, 517, 721, 1009
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 5 cut(s) 788, 794, 904, 917, 1057
MlyI GAGTC 3 cut(s) 575, 577, 936
MmeI TCCRAC 3 cut(s) 258, 594, 869
MroI TCCGGA 1 cut(s) 868
MseI TTAA 2 cut(s) 903, 1060
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 778
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 3 cut(s) 131, 667, 1031
MvnI CGCG 2 cut(s) 147, 354
MwoI GCNNNNNNNGC 1 cut(s) 781
NciI CCSGG 7 cut(s) 342, 553, 713, 827, 898, 899, 1005
NdeII GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
NlaIII CATG 1 cut(s) 685
NlaIV GGNNCC 2 cut(s) 696, 867
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 2 cut(s) 328, 746
OliI CACNNNNGTG 1 cut(s) 434
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 432, 854
PctI GAATGC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 648
PfoI TCCNGGA 1 cut(s) 1003
PkrI GCNGC 3 cut(s) 112, 664, 777
PleI GAGTC 3 cut(s) 574, 577, 935
PpsI GAGTC 3 cut(s) 574, 577, 935
PpuMI RGGWCCY 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 3 cut(s) 129, 665, 1029
PspGI CCWGG 3 cut(s) 129, 665, 1029
PspN4I GGNNCC 2 cut(s) 696, 867
PspPI GGNCC 3 cut(s) 133, 555, 694
PspPPI RGGWCCY 1 cut(s) 694
PstNI CAGNNNCTG 1 cut(s) 648
PsuI RGATCY 1 cut(s) 865
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 6 cut(s) 21, 482, 608, 659, 677, 880
RsaNI GTAC 6 cut(s) 20, 481, 607, 658, 676, 879
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 2 cut(s) 903, 1060
SatI GCNGC 3 cut(s) 111, 663, 776
Sau3AI GATC 7 cut(s) 102, 155, 313, 402, 445, 769, 865
Sau96I GGNCC 3 cut(s) 133, 555, 694
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 3 cut(s) 575, 577, 936
SduI GDGCHC 1 cut(s) 441
SfaNI GCATC 1 cut(s) 675
SinI GGWCC 1 cut(s) 694
SmaI CCCGGG 1 cut(s) 899
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 5 cut(s) 788, 794, 904, 917, 1057
SspI AATATT 1 cut(s) 298
TaaI ACNGT 3 cut(s) 359, 386, 913
TaiI ACGT 3 cut(s) 306, 330, 513
TaqI TCGA 8 cut(s) 123, 186, 441, 543, 621, 638, 702, 857
TasI AATT 5 cut(s) 788, 794, 904, 917, 1057
TatI WGTACW 2 cut(s) 480, 878
TauI GCSGC 2 cut(s) 113, 665
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 2 cut(s) 903, 1060
Tru9I TTAA 2 cut(s) 903, 1060
TscAI CASTG 1 cut(s) 1053
TseFI GTSAC 2 cut(s) 328, 746
TseI GCWGC 1 cut(s) 775
Tsp45I GTSAC 2 cut(s) 328, 746
TspDTI ATGAA 3 cut(s) 132, 352, 607
TspMI CCCGGG 1 cut(s) 897
TspRI CASTG 1 cut(s) 1053
Van91I CCANNNNNTGG 1 cut(s) 648
VpaK11BI GGWCC 1 cut(s) 694
XapI RAATTY 2 cut(s) 788, 917
XmaI CCCGGG 1 cut(s) 897
XmiI GTMKAC 1 cut(s) 513
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.