pycom03g11090

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016215
Physical Location & Seq
Reverse (-)
10719029 .. 10719661
633 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g11090.1

Sequence Viewer

Length: 633 bp
ATGTTCTTCGCCGTCCTCTCCCAGCACTGTGTCCCCCTCCACTCTTTTCGATACGTCTACCATTCCCTCTTCTTCTCCACCACCTTCGACTTGACCCGCCCCGCTGCCGATTCCGACGATGCCGAGTTGACTCGCATGGGACTCAAGATCCGCTACAAGAGCTTCATCGAGATCATCTCCAAATCCACCAATCTCTGGAAGCGGTACTCAGCCAGAGGACGCTTCGCGATGATGCCGGAGGTTCCATTCGATCAATTCCGGGTCGGATCTCAGTTTTTCCTCCTTACGCGCCATCACGCGCTCGTGGTGCTCAAGGATCGCGCGCTCTGGAGAAAGTTCAGAGTGCCGTGTTACAGAGAAGACGAATGCTACCCGGAAGAGCACTACTTTCCGACCTTGTTGTCAATGGCGGATCCGGAAGGGGTAACCCGTTACACTCTTACCCGTGTTAATTGGACGGGCACGGTTAACGGGCACCCGTACACGTACCGACCCGGGGAAGTGTCGGCGGAGTTGATTTATCGGCTGCGGAAGTCTAATTTCTCGGAGTCGTACTTGTTCGCGAGGAAGTTCTCGCCGGATTGCCTGAAACCCTTGTTGGATTTGGCCGATAAAGTCATTTTCCGGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

211

Amino Acids

24.84

Weight (kDa)

9.26

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 52 - 176 9.2e-27 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22070
fragaria_vesca FvH4_2g09440
malus_domestica MD03G1158600.v1.1
prunus_persica Prupe.6G146600_v2.0.a1 Prupe.6G146600_v2.0.a1
pyrus_communis pycom03g11090
rosa_chinensis RchiOBHm_Chr5g0048971
rosa_laevigata RLG00000034575
rosa_multiflora Rmu_sc0004058.1_g000017
rosa_rugosa Rorug05G0241600
rosa_samantha Rh5AG321800 Rh5BG332000 Rh5CG357300 Rh5DG343800
rosa_wichuraiana Rw5G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 474
AccB7I CCANNNNNTGG 1 cut(s) 195
AccI GTMKAC 1 cut(s) 57
AccII CGCG 6 cut(s) 227, 289, 299, 321, 323, 563
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 7 cut(s) 97, 102, 151, 202, 410, 509, 529
AclWI GGATC 5 cut(s) 142, 274, 324, 407, 420
AcoI YGGCCR 1 cut(s) 606
AfaI GTAC 4 cut(s) 206, 482, 488, 554
AfiI CCNNNNNNNGG 2 cut(s) 195, 496
AflIII ACRYGT 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AluBI AGCT 1 cut(s) 162
AluI AGCT 1 cut(s) 162
Alw21I GWGCWC 2 cut(s) 312, 384
AlwI GGATC 5 cut(s) 142, 274, 324, 407, 420
Ama87I CYCGRG 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 2 cut(s) 104, 526
AspLEI GCGC 4 cut(s) 291, 301, 323, 325
AsuC2I CCSGG 5 cut(s) 260, 374, 495, 496, 626
AvaI CYCGRG 1 cut(s) 494
BaeGI GKGCMC 2 cut(s) 464, 477
BamHI GGATCC 1 cut(s) 412
BanI GGYRCC 1 cut(s) 474
BauI CACGAG 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 2 cut(s) 312, 384
BbvI GCAGC 2 cut(s) 91, 513
BccI CCATC 1 cut(s) 300
BceAI ACGGC 1 cut(s) 331
BcnI CCSGG 5 cut(s) 260, 374, 495, 496, 626
BisI GCNGC 2 cut(s) 105, 527
BlsI GCNGC 2 cut(s) 106, 528
Bme1390I CCNGG 5 cut(s) 260, 374, 495, 496, 626
BmeT110I CYCGRG 1 cut(s) 494
BmiI GGNNCC 3 cut(s) 243, 414, 476
BmrFI CCNGG 5 cut(s) 260, 374, 495, 496, 626
BmsI GCATC 2 cut(s) 109, 222
BpiI GAAGAC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 161, 193
BpmI CTGGAG 1 cut(s) 349
BpuEI CTTGAG 2 cut(s) 128, 296
BpuMI CCSGG 5 cut(s) 260, 374, 495, 496, 626
BsaAI YACGTR 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 494, 495
BsaWI WCCGGW 1 cut(s) 415
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 496
BseAI TCCGGA 1 cut(s) 415
BseDI CCNNGG 2 cut(s) 494, 495
BseLI CCNNNNNNNGG 2 cut(s) 195, 496
BseMII CTCAG 2 cut(s) 222, 284
BsePI GCGCGC 1 cut(s) 321
BseSI GKGCMC 2 cut(s) 464, 477
BseXI GCAGC 2 cut(s) 91, 513
BseYI CCCAGC 1 cut(s) 21
Bsh1236I CGCG 6 cut(s) 227, 289, 299, 321, 323, 563
BshFI GGCC 1 cut(s) 608
BshNI GGYRCC 1 cut(s) 474
BsiHKAI GWGCWC 2 cut(s) 312, 384
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 7 cut(s) 236, 259, 374, 416, 495, 578, 625
BslFI GGGAC 2 cut(s) 17, 153
BslI CCNNNNNNNGG 2 cut(s) 195, 496
BsmFI GGGAC 2 cut(s) 17, 153
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 1 cut(s) 608
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 4 cut(s) 312, 384, 464, 477
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 6 cut(s) 147, 171, 250, 266, 316, 412
Bsp68I TCGCGA 2 cut(s) 227, 563
BspACI CCGC 7 cut(s) 97, 102, 151, 202, 410, 509, 529
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 2 cut(s) 221, 283
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 6 cut(s) 227, 289, 299, 321, 323, 563
BspLI GGNNCC 3 cut(s) 243, 414, 476
BspPI GGATC 5 cut(s) 142, 274, 324, 407, 420
BspQI GCTCTTC 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 474
BssECI CCNNGG 2 cut(s) 494, 495
BssHII GCGCGC 1 cut(s) 321
BssMI GATC 6 cut(s) 147, 171, 250, 266, 316, 412
BssSI CACGAG 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 29, 466
Bst6I CTCTTC 2 cut(s) 74, 372
BstBAI YACGTR 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 323
BstDEI CTNAG 2 cut(s) 208, 270
BstEII GGTNACC 1 cut(s) 424
BstFNI CGCG 6 cut(s) 227, 289, 299, 321, 323, 563
BstHHI GCGC 4 cut(s) 291, 301, 323, 325
BstKTI GATC 6 cut(s) 150, 174, 253, 269, 319, 415
BstMBI GATC 6 cut(s) 147, 171, 250, 266, 316, 412
BstMWI GCNNNNNNNGC 2 cut(s) 159, 307
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 5 cut(s) 258, 372, 493, 494, 624
BstSLI GKGCMC 2 cut(s) 464, 477
BstUI CGCG 6 cut(s) 227, 289, 299, 321, 323, 563
BstV1I GCAGC 2 cut(s) 91, 513
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 3 cut(s) 147, 266, 412
BstYI RGATCY 3 cut(s) 147, 266, 412
BsuRI GGCC 1 cut(s) 608
BtgZI GCGATG 1 cut(s) 242
BtsIMutI CAGTG 1 cut(s) 25
BtuMI TCGCGA 2 cut(s) 227, 563
Cac8I GCNNGC 1 cut(s) 323
CfoI GCGC 4 cut(s) 291, 301, 323, 325
Cfr9I CCCGGG 1 cut(s) 494
CseI GACGC 1 cut(s) 228
Csp6I GTAC 4 cut(s) 205, 481, 487, 553
CviAII CATG 1 cut(s) 136
CviJI RGCY 4 cut(s) 162, 212, 526, 608
CviKI_1 RGCY 4 cut(s) 162, 212, 526, 608
CviQI GTAC 4 cut(s) 205, 481, 487, 553
DdeI CTNAG 2 cut(s) 208, 270
DpnI GATC 6 cut(s) 149, 173, 252, 268, 318, 414
DpnII GATC 6 cut(s) 147, 171, 250, 266, 316, 412
DrdI GACNNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 2 cut(s) 74, 372
EarI CTCTTC 2 cut(s) 74, 372
EciI GGCGGA 2 cut(s) 425, 524
Eco88I CYCGRG 1 cut(s) 494
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
FaeI CATG 1 cut(s) 139
FaiI YATR 1 cut(s) 137
FaqI GGGAC 2 cut(s) 17, 153
FatI CATG 1 cut(s) 135
FauI CCCGC 2 cut(s) 104, 109
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 105, 527
Fsp4HI GCNGC 2 cut(s) 105, 527
GlaI GCGC 4 cut(s) 290, 300, 322, 324
GluI GCNGC 2 cut(s) 105, 527
GsaI CCCAGC 1 cut(s) 25
GsuI CTGGAG 1 cut(s) 349
HaeIII GGCC 1 cut(s) 608
HapII CCGG 7 cut(s) 236, 259, 374, 416, 495, 578, 625
HgaI GACGC 1 cut(s) 228
HhaI GCGC 4 cut(s) 291, 301, 323, 325
Hin1II CATG 1 cut(s) 139
Hin6I GCGC 4 cut(s) 289, 299, 321, 323
HinP1I GCGC 4 cut(s) 289, 299, 321, 323
HincII GTYRAC 2 cut(s) 129, 469
HindII GTYRAC 2 cut(s) 129, 469
HinfI GANTC 4 cut(s) 110, 130, 141, 548
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 7 cut(s) 236, 259, 374, 416, 495, 578, 625
Hpy166II GTNNAC 4 cut(s) 58, 129, 469, 483
Hpy188I TCNGA 5 cut(s) 115, 266, 341, 393, 547
Hpy188III TCNNGA 7 cut(s) 145, 169, 196, 226, 328, 416, 562
Hpy8I GTNNAC 4 cut(s) 58, 129, 469, 483
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 94, 413
HpyCH4III ACNGT 2 cut(s) 29, 466
HpyCH4IV ACGT 2 cut(s) 54, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 307
HpyF3I CTNAG 2 cut(s) 208, 270
HpySE526I ACGT 2 cut(s) 54, 485
Hsp92II CATG 1 cut(s) 139
HspAI GCGC 4 cut(s) 289, 299, 321, 323
Kpn2I TCCGGA 1 cut(s) 415
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 6 cut(s) 147, 171, 250, 266, 316, 412
LguI GCTCTTC 1 cut(s) 372
Lsp1109I GCAGC 2 cut(s) 91, 513
LweI GCATC 2 cut(s) 109, 222
MaeII ACGT 2 cut(s) 54, 485
MaeIII GTNAC 3 cut(s) 350, 424, 431
MalI GATC 6 cut(s) 149, 173, 252, 268, 318, 414
MboI GATC 6 cut(s) 147, 171, 250, 266, 316, 412
MboII GAAGA 4 cut(s) 61, 64, 371, 389
MflI RGATCY 3 cut(s) 147, 266, 412
MhlI GDGCHC 4 cut(s) 312, 384, 464, 477
MluCI AATT 3 cut(s) 254, 451, 538
MlyI GAGTC 3 cut(s) 124, 135, 557
MmeI TCCRAC 4 cut(s) 138, 244, 416, 579
MnlI CCTC 7 cut(s) 26, 47, 77, 209, 232, 290, 558
MroI TCCGGA 1 cut(s) 415
MseI TTAA 2 cut(s) 450, 468
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 7 cut(s) 236, 259, 374, 416, 495, 578, 625
MspR9I CCNGG 5 cut(s) 260, 374, 495, 496, 626
Mva1269I GAATGC 1 cut(s) 371
MvnI CGCG 6 cut(s) 227, 289, 299, 321, 323, 563
MwoI GCNNNNNNNGC 2 cut(s) 159, 307
NciI CCSGG 5 cut(s) 260, 374, 495, 496, 626
NdeII GATC 6 cut(s) 147, 171, 250, 266, 316, 412
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 3 cut(s) 243, 414, 476
NmeAIII GCCGAG 1 cut(s) 148
NruI TCGCGA 2 cut(s) 227, 563
PauI GCGCGC 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 624
PkrI GCNGC 2 cut(s) 106, 528
PleI GAGTC 3 cut(s) 124, 135, 556
PpsI GAGTC 3 cut(s) 124, 135, 556
Ppu21I YACGTR 1 cut(s) 486
PspEI GGTNACC 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 243, 414, 476
PsuI RGATCY 3 cut(s) 147, 266, 412
PteI GCGCGC 1 cut(s) 321
RruI TCGCGA 2 cut(s) 227, 563
RsaI GTAC 4 cut(s) 206, 482, 488, 554
RsaNI GTAC 4 cut(s) 205, 481, 487, 553
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 2 cut(s) 450, 468
SatI GCNGC 2 cut(s) 105, 527
Sau3AI GATC 6 cut(s) 147, 171, 250, 266, 316, 412
SchI GAGTC 3 cut(s) 124, 135, 557
ScrFI CCNGG 5 cut(s) 260, 374, 495, 496, 626
SduI GDGCHC 4 cut(s) 312, 384, 464, 477
SetI ASST 6 cut(s) 57, 86, 164, 243, 398, 488
SfaNI GCATC 2 cut(s) 109, 222
SmaI CCCGGG 1 cut(s) 496
SmlI CTYRAG 2 cut(s) 143, 311
SmoI CTYRAG 2 cut(s) 143, 311
Sse9I AATT 3 cut(s) 254, 451, 538
SsiI CCGC 7 cut(s) 97, 102, 151, 202, 410, 509, 529
StyD4I CCNGG 5 cut(s) 258, 372, 493, 494, 624
TaaI ACNGT 2 cut(s) 29, 466
TaiI ACGT 2 cut(s) 57, 488
TaqI TCGA 4 cut(s) 49, 87, 168, 249
TasI AATT 3 cut(s) 254, 451, 538
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 2 cut(s) 450, 468
Tru9I TTAA 2 cut(s) 450, 468
TscAI CASTG 1 cut(s) 32
TseI GCWGC 2 cut(s) 104, 526
TspDTI ATGAA 1 cut(s) 154
TspMI CCCGGG 1 cut(s) 494
TspRI CASTG 1 cut(s) 32
Van91I CCANNNNNTGG 1 cut(s) 195
XmaI CCCGGG 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.