MD03G1158600.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016215
Physical Location & Seq
Forward (+)
18836573 .. 18841218
4646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1158600.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGTTCTCCTCCACCCCATTTCTGGTCTCCTTCTTCCTCTTCCTTTCTCTCCCTCTCCTATTCCTCCTCGGCCCTCGCTTCCTCCCCCCCTCCCACCCCACCGTCCCCATTTCCCCCGCCGACGAAATCGACGACCAAATCCTCTTCTCCCGCGCCCTCAACCCTAACCCTAACCGACCCAAACCCACCTTCTCCCACCTCGCCCTCGATTCCAAATCCTCCAAGCCCAAAATTGCCTTCCTCTTCCTCACCAACACCGACCTCCACTTCGCCCCTCTCTGGTCCTGCTTCTTCTCCAAAACCAACCCCAATCTTTACAATATTTACGTCCATGCCGACCCCTCTGCCAACGTCACTCTTCCCCCCGGCACCGTCTTCCACAACCGCCTCGTCCCTTCCAAGCGCACCTACCGCGCCTCCGCCACCTTGATCTCCGCCACGCGCCGCCTTCTCGCCTCCGCCGTCATCGATGACCCTGCCAACATGTTCTTCGCCGTCCTCTCCCAGCACTGCGTCCCCCTCCACTCTTTTCGATACGTCTACCATTCCCTCTTCTTCTCCACCACCTTCGACTTGACCCGCCCCGCTGCCGACTCCGACGATGCCGAGTTGACCCGGATGGGACTCAAGATCCGCTACAAGAGCTTCATCGAGATCATCTCCAAATCCACCAATCTCTGGAAGCGGTACTCCGCCCGAGGACGCTTCGCGATGATGCCGGAGGTTCCATTCGATCAATTCCGGGTCGGATCCCAGTTCTTCCTCCTTACGCGCCATCACGCGCTCGTGGTGCTCAAGGATCGCGCGCTCTGGAGAAAGTTCAGAGTGCCGTGTTACAGAGAAGACGAATGCTACCCGGAAGAGCACTACTTTCCGACCTTGTTGTCAATGGCGGATCCGGAAGGGGTAACCCGTTACACTCTTACCCGGGTTAATTGGACGGGCACGGTTAACGGGCACCCGTACACGTACCGACCCGTGGAAGTGTCGGCGGAGTTGATTTATCGGCTGCGGAAGTCGAATTTCTCGGAGTCGTACTTGTTCGCGAGGAAGTTCTCGCCGGATTGCCTGAAACCCTTGTTGGATTTGGCCGATAAAGTCATTTTCCGGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

372

Amino Acids

42.77

Weight (kDa)

9.48

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 78 - 337 6.7e-52 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22070
fragaria_vesca FvH4_2g09440
malus_domestica MD03G1158600.v1.1
prunus_persica Prupe.6G146600_v2.0.a1 Prupe.6G146600_v2.0.a1
pyrus_communis pycom03g11090
rosa_chinensis RchiOBHm_Chr5g0048971
rosa_laevigata RLG00000034575
rosa_multiflora Rmu_sc0004058.1_g000017
rosa_rugosa Rorug05G0241600
rosa_samantha Rh5AG321800 Rh5BG332000 Rh5CG357300 Rh5DG343800
rosa_wichuraiana Rw5G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 883
AccB1I GGYRCC 2 cut(s) 368, 957
AccB7I CCANNNNNTGG 1 cut(s) 678
AccI GTMKAC 1 cut(s) 540
AccII CGCG 9 cut(s) 153, 414, 442, 710, 772, 782, 804, 806, 1046
AccIII TCCGGA 1 cut(s) 898
AclWI GGATC 6 cut(s) 625, 744, 757, 807, 890, 903
AcoI YGGCCR 1 cut(s) 1089
AcsI RAATTY 1 cut(s) 1021
AfaI GTAC 4 cut(s) 689, 965, 971, 1037
AfiI CCNNNNNNNGG 4 cut(s) 22, 279, 678, 979
AflIII ACRYGT 2 cut(s) 483, 966
AjuI GAANNNNNNNTTGG 8 cut(s) 173, 205, 221, 253, 473, 505, 1064, 1096
AluBI AGCT 1 cut(s) 645
AluI AGCT 1 cut(s) 645
Alw21I GWGCWC 2 cut(s) 795, 867
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 6 cut(s) 625, 744, 757, 807, 890, 903
Ama87I CYCGRG 2 cut(s) 696, 927
Aor13HI TCCGGA 1 cut(s) 898
AoxI GGCC 2 cut(s) 70, 1089
ApeKI GCWGC 2 cut(s) 587, 1009
ApoI RAATTY 1 cut(s) 1021
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AspLEI GCGC 8 cut(s) 155, 405, 416, 444, 774, 784, 806, 808
AspS9I GGNCC 2 cut(s) 71, 282
AsuC2I CCSGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 2 cut(s) 696, 927
AvaII GGWCC 1 cut(s) 282
BaeGI GKGCMC 2 cut(s) 947, 960
BamHI GGATCC 2 cut(s) 749, 895
BanI GGYRCC 2 cut(s) 368, 957
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 367, 849
Bbv12I GWGCWC 2 cut(s) 795, 867
BbvI GCAGC 2 cut(s) 574, 996
BccI CCATC 2 cut(s) 613, 783
BceAI ACGGC 3 cut(s) 446, 479, 814
BcgI CGANNNNNNTGC 4 cut(s) 326, 360, 458, 492
BcnI CCSGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
BcoDI GTCTC 1 cut(s) 31
BisI GCNGC 3 cut(s) 445, 588, 1010
BlsI GCNGC 3 cut(s) 446, 589, 1011
Bme1390I CCNGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 2 cut(s) 696, 927
BmgT120I GGNCC 2 cut(s) 71, 282
BmiI GGNNCC 5 cut(s) 370, 726, 751, 897, 959
BmrFI CCNGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
BmrI ACTGGG 1 cut(s) 748
BmsI GCATC 2 cut(s) 592, 705
BmuI ACTGGG 1 cut(s) 748
BpiI GAAGAC 2 cut(s) 367, 849
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 1 cut(s) 832
BpuEI CTTGAG 2 cut(s) 611, 779
BpuMI CCSGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
Bsa29I ATCGAT 1 cut(s) 468
BsaAI YACGTR 1 cut(s) 969
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 5 cut(s) 67, 364, 697, 927, 978
BsaWI WCCGGW 1 cut(s) 898
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 279, 678, 979
Bse1I ACTGG 1 cut(s) 754
BseAI TCCGGA 1 cut(s) 898
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 5 cut(s) 67, 364, 697, 927, 978
BseGI GGATG 1 cut(s) 624
BseLI CCNNNNNNNGG 4 cut(s) 22, 279, 678, 979
BseNI ACTGG 1 cut(s) 754
BsePI GCGCGC 1 cut(s) 804
BseRI GAGGAG 1 cut(s) 56
BseSI GKGCMC 2 cut(s) 947, 960
BseXI GCAGC 2 cut(s) 574, 996
BseYI CCCAGC 1 cut(s) 504
Bsh1236I CGCG 9 cut(s) 153, 414, 442, 710, 772, 782, 804, 806, 1046
BshFI GGCC 2 cut(s) 72, 1091
BshNI GGYRCC 2 cut(s) 368, 957
BshVI ATCGAT 1 cut(s) 468
BsiHKAI GWGCWC 2 cut(s) 795, 867
BsiHKCI CYCGRG 2 cut(s) 696, 927
BsiSI CCGG 9 cut(s) 366, 616, 719, 742, 857, 899, 928, 1061, 1108
BslFI GGGAC 4 cut(s) 89, 377, 500, 636
BslI CCNNNNNNNGG 4 cut(s) 22, 279, 678, 979
BsmAI GTCTC 1 cut(s) 31
BsmFI GGGAC 4 cut(s) 89, 377, 500, 636
BsmI GAATGC 1 cut(s) 854
BsnI GGCC 2 cut(s) 72, 1091
Bso31I GGTCTC 1 cut(s) 31
BsoBI CYCGRG 2 cut(s) 696, 927
Bsp1286I GDGCHC 4 cut(s) 795, 867, 947, 960
Bsp13I TCCGGA 1 cut(s) 898
Bsp143I GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
Bsp68I TCGCGA 2 cut(s) 710, 1046
BspANI GGCC 2 cut(s) 72, 1091
BspDI ATCGAT 1 cut(s) 468
BspEI TCCGGA 1 cut(s) 898
BspFNI CGCG 9 cut(s) 153, 414, 442, 710, 772, 782, 804, 806, 1046
BspLI GGNNCC 5 cut(s) 370, 726, 751, 897, 959
BspPI GGATC 6 cut(s) 625, 744, 757, 807, 890, 903
BspQI GCTCTTC 1 cut(s) 855
BspT107I GGYRCC 2 cut(s) 368, 957
BspTNI GGTCTC 1 cut(s) 31
BsrI ACTGG 1 cut(s) 754
BssECI CCNNGG 5 cut(s) 67, 364, 697, 927, 978
BssHII GCGCGC 1 cut(s) 804
BssMI GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
BssSI CACGAG 1 cut(s) 785
Bst2BI CACGAG 1 cut(s) 785
Bst4CI ACNGT 3 cut(s) 103, 373, 949
Bst6I CTCTTC 6 cut(s) 44, 149, 248, 363, 557, 855
BstBAI YACGTR 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 806
BstDSI CCRYGG 1 cut(s) 978
BstEII GGTNACC 1 cut(s) 907
BstF5I GGATG 1 cut(s) 624
BstFNI CGCG 9 cut(s) 153, 414, 442, 710, 772, 782, 804, 806, 1046
BstHHI GCGC 8 cut(s) 155, 405, 416, 444, 774, 784, 806, 808
BstKTI GATC 7 cut(s) 432, 633, 657, 736, 752, 802, 898
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
BstMWI GCNNNNNNNGC 3 cut(s) 411, 642, 790
BstNSI RCATGY 1 cut(s) 487
BstPI GGTNACC 1 cut(s) 907
BstSCI CCNGG 7 cut(s) 364, 614, 741, 855, 926, 927, 1107
BstSLI GKGCMC 2 cut(s) 947, 960
BstUI CGCG 9 cut(s) 153, 414, 442, 710, 772, 782, 804, 806, 1046
BstV1I GCAGC 2 cut(s) 574, 996
BstV2I GAAGAC 2 cut(s) 367, 849
BstX2I RGATCY 3 cut(s) 630, 749, 895
BstYI RGATCY 3 cut(s) 630, 749, 895
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 2 cut(s) 72, 1091
BsuTUI ATCGAT 1 cut(s) 468
BtgI CCRYGG 1 cut(s) 978
BtgZI GCGATG 1 cut(s) 725
BtsCI GGATG 1 cut(s) 624
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 1 cut(s) 508
BtuMI TCGCGA 2 cut(s) 710, 1046
Cac8I GCNNGC 1 cut(s) 806
CfoI GCGC 8 cut(s) 155, 405, 416, 444, 774, 784, 806, 808
Cfr13I GGNCC 2 cut(s) 71, 282
Cfr9I CCCGGG 1 cut(s) 927
ClaI ATCGAT 1 cut(s) 468
CseI GACGC 2 cut(s) 502, 711
Csp6I GTAC 4 cut(s) 688, 964, 970, 1036
CviAII CATG 2 cut(s) 332, 484
CviJI RGCY 5 cut(s) 72, 226, 645, 1009, 1091
CviKI_1 RGCY 5 cut(s) 72, 226, 645, 1009, 1091
CviQI GTAC 4 cut(s) 688, 964, 970, 1036
DpnI GATC 7 cut(s) 431, 632, 656, 735, 751, 801, 897
DpnII GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
DrdI GACNNNNNNGTC 1 cut(s) 883
DseDI GACNNNNNNGTC 1 cut(s) 883
EaeI YGGCCR 1 cut(s) 1089
Eam1104I CTCTTC 6 cut(s) 44, 149, 248, 363, 557, 855
EarI CTCTTC 6 cut(s) 44, 149, 248, 363, 557, 855
EciI GGCGGA 6 cut(s) 409, 424, 448, 682, 908, 1007
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 696, 927
Eco91I GGTNACC 1 cut(s) 907
EcoO65I GGTNACC 1 cut(s) 907
FaeI CATG 2 cut(s) 335, 487
FaiI YATR 2 cut(s) 333, 485
FaqI GGGAC 4 cut(s) 89, 377, 500, 636
FatI CATG 2 cut(s) 331, 483
FauI CCCGC 4 cut(s) 124, 158, 587, 592
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 3 cut(s) 445, 588, 1010
FokI GGATG 1 cut(s) 631
Fsp4HI GCNGC 3 cut(s) 445, 588, 1010
GlaI GCGC 8 cut(s) 154, 404, 415, 443, 773, 783, 805, 807
GluI GCNGC 3 cut(s) 445, 588, 1010
GsaI CCCAGC 1 cut(s) 508
GsuI CTGGAG 1 cut(s) 832
HaeIII GGCC 2 cut(s) 72, 1091
HapII CCGG 9 cut(s) 366, 616, 719, 742, 857, 899, 928, 1061, 1108
HgaI GACGC 2 cut(s) 502, 711
HhaI GCGC 8 cut(s) 155, 405, 416, 444, 774, 784, 806, 808
Hin1II CATG 2 cut(s) 335, 487
Hin6I GCGC 8 cut(s) 153, 403, 414, 442, 772, 782, 804, 806
HinP1I GCGC 8 cut(s) 153, 403, 414, 442, 772, 782, 804, 806
HincII GTYRAC 2 cut(s) 612, 952
HindII GTYRAC 2 cut(s) 612, 952
HinfI GANTC 4 cut(s) 209, 593, 624, 1031
HpaI GTTAAC 1 cut(s) 952
HpaII CCGG 9 cut(s) 366, 616, 719, 742, 857, 899, 928, 1061, 1108
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 4 cut(s) 541, 612, 952, 966
Hpy188I TCNGA 5 cut(s) 598, 749, 824, 876, 1030
Hpy188III TCNNGA 7 cut(s) 628, 652, 679, 709, 811, 899, 1045
Hpy8I GTNNAC 4 cut(s) 541, 612, 952, 966
Hpy99I CGWCG 3 cut(s) 125, 134, 602
HpyAV CCTTC 7 cut(s) 40, 199, 247, 405, 458, 577, 896
HpyCH4III ACNGT 3 cut(s) 103, 373, 949
HpyCH4IV ACGT 4 cut(s) 327, 351, 537, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 411, 642, 790
HpySE526I ACGT 4 cut(s) 327, 351, 537, 968
Hsp92II CATG 2 cut(s) 335, 487
HspAI GCGC 8 cut(s) 153, 403, 414, 442, 772, 782, 804, 806
Kpn2I TCCGGA 1 cut(s) 898
KspAI GTTAAC 1 cut(s) 952
Kzo9I GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
LguI GCTCTTC 1 cut(s) 855
Lsp1109I GCAGC 2 cut(s) 574, 996
LweI GCATC 2 cut(s) 592, 705
MaeII ACGT 4 cut(s) 327, 351, 537, 968
MaeIII GTNAC 4 cut(s) 352, 833, 907, 914
MalI GATC 7 cut(s) 431, 632, 656, 735, 751, 801, 897
MboI GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
MflI RGATCY 3 cut(s) 630, 749, 895
MhlI GDGCHC 4 cut(s) 795, 867, 947, 960
MluCI AATT 4 cut(s) 231, 737, 934, 1021
MlyI GAGTC 3 cut(s) 587, 618, 1040
MmeI TCCRAC 4 cut(s) 621, 727, 899, 1062
MroI TCCGGA 1 cut(s) 898
MseI TTAA 2 cut(s) 933, 951
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 9 cut(s) 366, 616, 719, 742, 857, 899, 928, 1061, 1108
MspR9I CCNGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
Mva1269I GAATGC 1 cut(s) 854
MvnI CGCG 9 cut(s) 153, 414, 442, 710, 772, 782, 804, 806, 1046
MwoI GCNNNNNNNGC 3 cut(s) 411, 642, 790
NciI CCSGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
NdeII GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
NlaIII CATG 2 cut(s) 335, 487
NlaIV GGNNCC 5 cut(s) 370, 726, 751, 897, 959
NmeAIII GCCGAG 2 cut(s) 48, 631
NmuCI GTSAC 1 cut(s) 352
NruI TCGCGA 2 cut(s) 710, 1046
NspI RCATGY 1 cut(s) 487
PauI GCGCGC 1 cut(s) 804
PciI ACATGT 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 855
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 333, 459
PctI GAATGC 1 cut(s) 854
PfeI GAWTC 1 cut(s) 209
PflMI CCANNNNNTGG 1 cut(s) 678
PfoI TCCNGGA 1 cut(s) 1107
PkrI GCNGC 3 cut(s) 446, 589, 1011
PleI GAGTC 3 cut(s) 587, 618, 1039
PpsI GAGTC 3 cut(s) 587, 618, 1039
Ppu21I YACGTR 1 cut(s) 969
PscI ACATGT 1 cut(s) 483
PspEI GGTNACC 1 cut(s) 907
PspFI CCCAGC 1 cut(s) 504
PspN4I GGNNCC 5 cut(s) 370, 726, 751, 897, 959
PspPI GGNCC 2 cut(s) 71, 282
PsuI RGATCY 3 cut(s) 630, 749, 895
PteI GCGCGC 1 cut(s) 804
RruI TCGCGA 2 cut(s) 710, 1046
RsaI GTAC 4 cut(s) 689, 965, 971, 1037
RsaNI GTAC 4 cut(s) 688, 964, 970, 1036
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 2 cut(s) 933, 951
SatI GCNGC 3 cut(s) 445, 588, 1010
Sau3AI GATC 7 cut(s) 429, 630, 654, 733, 749, 799, 895
Sau96I GGNCC 2 cut(s) 71, 282
SchI GAGTC 3 cut(s) 587, 618, 1040
ScrFI CCNGG 7 cut(s) 366, 616, 743, 857, 928, 929, 1109
SduI GDGCHC 4 cut(s) 795, 867, 947, 960
SfaNI GCATC 2 cut(s) 592, 705
SinI GGWCC 1 cut(s) 282
SmaI CCCGGG 1 cut(s) 929
SmlI CTYRAG 2 cut(s) 626, 794
SmoI CTYRAG 2 cut(s) 626, 794
Sse9I AATT 4 cut(s) 231, 737, 934, 1021
SspI AATATT 1 cut(s) 322
StyD4I CCNGG 7 cut(s) 364, 614, 741, 855, 926, 927, 1107
TaaI ACNGT 3 cut(s) 103, 373, 949
TaiI ACGT 4 cut(s) 330, 354, 540, 971
TaqI TCGA 8 cut(s) 129, 207, 468, 532, 570, 651, 732, 1019
TasI AATT 4 cut(s) 231, 737, 934, 1021
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 933, 951
Tru9I TTAA 2 cut(s) 933, 951
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 587, 1009
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 637
TspMI CCCGGG 1 cut(s) 927
TspRI CASTG 1 cut(s) 515
Van91I CCANNNNNTGG 1 cut(s) 678
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 1 cut(s) 1021
XceI RCATGY 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmaI CCCGGG 1 cut(s) 927
XmiI GTMKAC 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.