AT5G24020

septum site-determining protein minD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017186
Physical Location & Seq
Forward (+)
8116665 .. 8118087
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24020.1

Sequence Viewer

Length: 981 bp
ATGGCGTCTCTGAGATTGTTCTCAACGAATCATCAATCTCTTCTCCTTCCATCATCTCTCTCACAAAAGACTCTAATATCTTCACCAAGATTCGTCAATAACCCTAGCAGACGGAGTCCAATACGATCCGTTCTTCAATTTAATCGCAAACCGGAACTCGCCGGAGAAACGCCGCGTATCGTCGTTATCACCTCCGGAAAAGGCGGTGTTGGAAAGACGACAACCACCGCAAATGTCGGTCTCTCTCTCGCTCGTTACGGTTTCTCAGTTGTCGCCATTGACGCCGACCTTGGTCTCCGTAACCTCGATCTCCTCCTAGGGTTAGAGAATCGAGTCAATTACACTTGCGTCGAGGTTATAAACGGAGATTGTCGTCTCGATCAAGCTCTGGTACGTGATAAGCGTTGGTCGAATTTCGAATTGCTATGTATATCTAAACCTAGATCGAAACTTCCGATGGGATTTGGTGGTAAAGCATTGGAATGGCTTGTGGATGCGTTGAAAACTAGACCGGAAGGTTCACCGGATTTCATCATCATCGATTGTCCTGCAGGAATCGATGCCGGATTCATAACCGCCATTACTCCGGCGAATGAAGCAGTTCTGGTAACAACTCCGGATATAACAGCGTTAAGGGATGCTGATAGGGTTACGGGTTTGTTAGAATGCGATGGAATCAGAGATATAAAGATGATTGTGAACAGAGTGAGAACTGATATGATTAAAGGAGAGGATATGATGTCAGTGTTAGATGTGCAGGAGATGTTGGGATTGTCATTGCTTGGTGTAATTCCTGAAGATTCTGAGGTTATTCGAAGCACGAATCGAGGGTTTCCGCTTGTTCTGAATAAGCCTCCTACGCTTGCGGGATTGGCGTTTGAGCAGGCGGCTTGGAGACTCGTTGAGCAAGATAGTATGAAGGCTGTTATGGTGGAGGAAGAACCTAAGAAACGTGGCTTCTTCTCTTTCTTTGGCGGCTAA

Protein Analysis

326

Amino Acids

35.69

Weight (kDa)

7.58

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 59 - 297 7.3e-18 NUBPL iron-transfer P-loop NTPase
AAA_31 PF13614 59 - 212 1.3e-14 AAA domain
MipZ PF09140 59 - 198 1.5e-07 ATPase MipZ
CbiA PF01656 61 - 277 2.2e-20 CobQ/CobB/MinD/ParA nucleotide binding domain
Fer4_NifH PF00142 64 - 304 8.9e-07 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24020
fragaria_vesca FvH4_7g30210
malus_domestica MD07G1278700.v1.1
prunus_persica Prupe.2G300600_v2.0.a1
pyrus_communis pycom07g25360
rosa_chinensis RchiOBHm_Chr1g0378251
rosa_laevigata RLG00000026456
rosa_multiflora Rmu_sc0006315.1_g000003 Rmu_sc0038642.1_g000001
rosa_roxburghii Rroxscaffold_4G00280610
rosa_rugosa Rorug01G0407500
rosa_samantha Rh1AG422300
rosa_wichuraiana Rw1G037530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
AccII CGCG 1 cut(s) 175
AccIII TCCGGA 2 cut(s) 194, 616
AciI CCGC 8 cut(s) 173, 204, 228, 576, 836, 866, 887, 975
AclWI GGATC 1 cut(s) 120
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 1 cut(s) 816
AcyI GRCGYC 2 cut(s) 5, 282
AfaI GTAC 1 cut(s) 393
AgsI TTSAA 2 cut(s) 137, 502
AluBI AGCT 1 cut(s) 386
AluI AGCT 1 cut(s) 386
Alw26I GTCTC 5 cut(s) 12, 245, 299, 380, 889
AlwI GGATC 1 cut(s) 120
Aor13HI TCCGGA 2 cut(s) 194, 616
ApoI RAATTY 1 cut(s) 412
Asp700I GAANNNNTTC 1 cut(s) 600
AspA2I CCTAGG 1 cut(s) 316
AsuHPI GGTGA 3 cut(s) 75, 181, 513
AsuII TTCGAA 2 cut(s) 417, 814
AvrII CCTAGG 1 cut(s) 316
BccI CCATC 3 cut(s) 58, 451, 665
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BcoDI GTCTC 5 cut(s) 12, 245, 299, 380, 889
BfaI CTAG 4 cut(s) 105, 317, 441, 507
BfmI CTRYAG 1 cut(s) 549
BisI GCNGC 3 cut(s) 173, 888, 976
BlnI CCTAGG 1 cut(s) 316
BlsI GCNGC 3 cut(s) 174, 889, 977
BmsI GCATC 3 cut(s) 484, 550, 628
BoxI GACNNNNGTC 1 cut(s) 291
Bpu14I TTCGAA 2 cut(s) 417, 814
Bsa29I ATCGAT 2 cut(s) 540, 558
BsaAI YACGTR 1 cut(s) 395
BsaHI GRCGYC 2 cut(s) 5, 282
BsaI GGTCTC 2 cut(s) 245, 299
BsaJI CCNNGG 2 cut(s) 289, 316
BsaWI WCCGGW 5 cut(s) 151, 194, 511, 523, 616
Bse3DI GCAATG 1 cut(s) 776
BseAI TCCGGA 2 cut(s) 194, 616
BseCI ATCGAT 2 cut(s) 540, 558
BseDI CCNNGG 2 cut(s) 289, 316
BseGI GGATG 2 cut(s) 499, 643
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 2 cut(s) 279, 795
BseRI GAGGAG 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 1 cut(s) 175
BshVI ATCGAT 2 cut(s) 540, 558
BsiSI CCGG 8 cut(s) 152, 162, 195, 512, 524, 564, 587, 617
BsmAI GTCTC 5 cut(s) 12, 245, 299, 380, 889
BsmBI CGTCTC 2 cut(s) 12, 380
BsmI GAATGC 1 cut(s) 671
Bso31I GGTCTC 2 cut(s) 245, 299
Bsp119I TTCGAA 2 cut(s) 417, 814
Bsp13I TCCGGA 2 cut(s) 194, 616
Bsp143I GATC 4 cut(s) 125, 307, 379, 443
BspACI CCGC 8 cut(s) 173, 204, 228, 576, 836, 866, 887, 975
BspCNI CTCAG 2 cut(s) 278, 796
BspDI ATCGAT 2 cut(s) 540, 558
BspEI TCCGGA 2 cut(s) 194, 616
BspFNI CGCG 1 cut(s) 175
BspMAI CTGCAG 1 cut(s) 553
BspPI GGATC 1 cut(s) 120
BspT104I TTCGAA 2 cut(s) 417, 814
BspTNI GGTCTC 2 cut(s) 245, 299
BsrDI GCAATG 1 cut(s) 776
BssECI CCNNGG 2 cut(s) 289, 316
BssMI GATC 4 cut(s) 125, 307, 379, 443
BssNI GRCGYC 2 cut(s) 5, 282
BssT1I CCWWGG 2 cut(s) 289, 316
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 2 cut(s) 5, 282
BstBAI YACGTR 1 cut(s) 395
BstBI TTCGAA 2 cut(s) 417, 814
BstC8I GCNNGC 2 cut(s) 864, 885
BstDEI CTNAG 4 cut(s) 11, 265, 804, 945
BstF5I GGATG 2 cut(s) 499, 643
BstFNI CGCG 1 cut(s) 175
BstKTI GATC 4 cut(s) 128, 310, 382, 446
BstMAI GTCTC 5 cut(s) 12, 245, 299, 380, 889
BstMBI GATC 4 cut(s) 125, 307, 379, 443
BstMWI GCNNNNNNNGC 4 cut(s) 281, 596, 859, 872
BstPAI GACNNNNGTC 1 cut(s) 291
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 1 cut(s) 175
Bsu15I ATCGAT 2 cut(s) 540, 558
BsuTUI ATCGAT 2 cut(s) 540, 558
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 2 cut(s) 499, 643
BtsIMutI CAGTG 1 cut(s) 750
Cac8I GCNNGC 2 cut(s) 864, 885
ClaI ATCGAT 2 cut(s) 540, 558
CseI GACGC 2 cut(s) 290, 337
Csp6I GTAC 1 cut(s) 392
CviJI RGCY 7 cut(s) 386, 487, 853, 890, 923, 957, 978
CviKI_1 RGCY 7 cut(s) 386, 487, 853, 890, 923, 957, 978
CviQI GTAC 1 cut(s) 392
DdeI CTNAG 4 cut(s) 11, 265, 804, 945
DpnI GATC 4 cut(s) 127, 309, 381, 445
DpnII GATC 4 cut(s) 125, 307, 379, 443
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 289, 316
Eco31I GGTCTC 2 cut(s) 245, 299
Eco57I CTGAAG 1 cut(s) 816
EcoT14I CCWWGG 2 cut(s) 289, 316
ErhI CCWWGG 2 cut(s) 289, 316
Esp3I CGTCTC 2 cut(s) 12, 380
FauI CCCGC 1 cut(s) 859
Fnu4HI GCNGC 3 cut(s) 173, 888, 976
FokI GGATG 2 cut(s) 506, 650
Fsp4HI GCNGC 3 cut(s) 173, 888, 976
FspBI CTAG 4 cut(s) 105, 317, 441, 507
GluI GCNGC 3 cut(s) 173, 888, 976
HapII CCGG 8 cut(s) 152, 162, 195, 512, 524, 564, 587, 617
HgaI GACGC 2 cut(s) 290, 337
Hin1I GRCGYC 2 cut(s) 5, 282
HpaII CCGG 8 cut(s) 152, 162, 195, 512, 524, 564, 587, 617
HphI GGTGA 3 cut(s) 75, 181, 513
Hpy166II GTNNAC 2 cut(s) 521, 700
Hpy188I TCNGA 5 cut(s) 12, 456, 680, 805, 846
Hpy188III TCNNGA 4 cut(s) 195, 377, 617, 794
Hpy8I GTNNAC 2 cut(s) 521, 700
Hpy99I CGWCG 2 cut(s) 185, 353
HpyAV CCTTC 3 cut(s) 56, 509, 913
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 2 cut(s) 394, 952
HpyCH4V TGCA 2 cut(s) 551, 757
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 596, 859, 872
HpyF3I CTNAG 4 cut(s) 11, 265, 804, 945
HpySE526I ACGT 2 cut(s) 394, 952
Hsp92I GRCGYC 2 cut(s) 5, 282
Kpn2I TCCGGA 2 cut(s) 194, 616
Kzo9I GATC 4 cut(s) 125, 307, 379, 443
LweI GCATC 3 cut(s) 484, 550, 628
MaeI CTAG 4 cut(s) 105, 317, 441, 507
MaeII ACGT 2 cut(s) 394, 952
MaeIII GTNAC 4 cut(s) 254, 299, 607, 649
MalI GATC 4 cut(s) 127, 309, 381, 445
MboI GATC 4 cut(s) 125, 307, 379, 443
MboII GAAGA 6 cut(s) 32, 72, 125, 809, 950, 952
MluCI AATT 5 cut(s) 137, 337, 412, 419, 789
MlyI GAGTC 4 cut(s) 64, 124, 342, 891
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 9 cut(s) 202, 314, 323, 346, 724, 799, 821, 864, 928
MroI TCCGGA 2 cut(s) 194, 616
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 3 cut(s) 141, 632, 723
MslI CAYNNNNRTG 1 cut(s) 481
MspI CCGG 8 cut(s) 152, 162, 195, 512, 524, 564, 587, 617
Mva1269I GAATGC 1 cut(s) 671
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 4 cut(s) 281, 596, 859, 872
NdeII GATC 4 cut(s) 125, 307, 379, 443
NspV TTCGAA 2 cut(s) 417, 814
PcsI WCGNNNNNNNCGW 2 cut(s) 400, 452
PctI GAATGC 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 8 cut(s) 28, 90, 328, 555, 567, 675, 800, 823
PflFI GACNNNGTC 1 cut(s) 114
PkrI GCNGC 3 cut(s) 174, 889, 977
PleI GAGTC 4 cut(s) 64, 123, 341, 891
PpsI GAGTC 4 cut(s) 64, 123, 341, 891
Ppu21I YACGTR 1 cut(s) 395
PshAI GACNNNNGTC 1 cut(s) 291
PsiI TTATAA 1 cut(s) 359
PstI CTGCAG 1 cut(s) 553
PsyI GACNNNGTC 1 cut(s) 114
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
RseI CAYNNNNRTG 1 cut(s) 481
SaqAI TTAA 3 cut(s) 141, 632, 723
SatI GCNGC 3 cut(s) 173, 888, 976
Sau3AI GATC 4 cut(s) 125, 307, 379, 443
SbfI CCTGCAGG 1 cut(s) 553
SchI GAGTC 4 cut(s) 64, 124, 342, 891
SdaI CCTGCAGG 1 cut(s) 553
SfaNI GCATC 3 cut(s) 484, 550, 628
SfcI CTRYAG 1 cut(s) 549
SfuI TTCGAA 2 cut(s) 417, 814
SmiMI CAYNNNNRTG 1 cut(s) 481
Sse8387I CCTGCAGG 1 cut(s) 553
Sse9I AATT 5 cut(s) 137, 337, 412, 419, 789
SsiI CCGC 8 cut(s) 173, 204, 228, 576, 836, 866, 887, 975
SspMI CTAG 4 cut(s) 105, 317, 441, 507
StyI CCWWGG 2 cut(s) 289, 316
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 2 cut(s) 397, 955
TaqII GACCGA 1 cut(s) 227
TasI AATT 5 cut(s) 137, 337, 412, 419, 789
TauI GCSGC 3 cut(s) 175, 890, 978
TfiI GAWTC 8 cut(s) 28, 90, 328, 555, 567, 675, 800, 823
Tru1I TTAA 3 cut(s) 141, 632, 723
Tru9I TTAA 3 cut(s) 141, 632, 723
TscAI CASTG 1 cut(s) 750
TspDTI ATGAA 4 cut(s) 520, 559, 609, 932
TspGWI ACGGA 4 cut(s) 118, 127, 287, 378
TspRI CASTG 1 cut(s) 750
Tth111I GACNNNGTC 1 cut(s) 114
XapI RAATTY 1 cut(s) 412
XmaJI CCTAGG 1 cut(s) 316
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 4 cut(s) 105, 317, 441, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.