AT5G25310

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012970
Physical Location & Seq
Forward (+)
8784747 .. 8787366
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25310.1

Sequence Viewer

Length: 1443 bp
ATGGATAAGTTTCAATCCAAATTCACGAGATTCGGATTCATCTCGATTTGTTTCGGATCGATCGCACTTGTATTATTGATCTCTCATTGTTCAACTTCTTTCTTTGATTACTCTTTTCAGAAATTCAAATTCTCGTTCCCAGAAGAAACTGAGCTTCGAAGAAACGTTTACACTAGTAGTTCCGGTGAAGAAAACAGAGTCGTCGTTGATTCTCGTCATGTTTCTCAACAGATCCTCACTGTCCGATCTACTAACTCCACTTTACAGAGTAAACCGGAAAAACTAAACCGGAGAAATTTGGTGGAACAAGGATTAGCGAAAGCAAGAGCATCGATTTTAGAAGCTTCTTCGAATGTTAACACTACATTGTTCAAATCTGATCTTCCAAACTCAGAAATTTATCGTAATCCATCAGCTTTATATCGGAGTTACTTAGAAATGGAGAAGAGGTTTAAGGTGTATGTATACGAAGAAGGCGAGCCACCGCTAGTTCACGATGGACCGTGTAAGAGTGTTTACGCGGTTGAAGGGAGATTCATAACTGAGATGGAGAAAAGAAGGACTAAGTTTCGTACTTATGATCCTAACCAAGCTTATGTTTACTTCTTGCCATTTAGTGTTACTTGGCTGGTTCGATACTTATACGAAGGCAATTCTGATGCTAAGCCTTTGAAAACATTTGTTTCCGATTATATCAGATTAGTATCTACTAATCATCCTTTTTGGAATCGAACTAATGGTGCTGATCACTTCATGCTTACTTGTCACGATTGGGGACCTCTGACTTCACAAGCTAATAGAGATTTGTTCAACACTTCGATTCGAGTTATGTGTAATGCTAATTCATCAGAAGGGTTTAATCCTACTAAAGATGTGACTCTTCCTGAGATCAAACTCTATGGTGGTGAAGTTGATCACAAACTCAGGTTATCTAAAACCTTATCCGCTTCTCCGCGGCCTTACCTCGGGTTCTTCGCAGGCGGTGTCCACGGTCCGGTTAGACCGATTCTTTTAAAACACTGGAAACAGCGTGACCTGGATATGCCTGTTTATGAATACCTTCCTAAACACTTAAACTACTATGATTTCATGAGAAGTTCAAAGTTCTGTTTTTGTCCCAGTGGGTATGAGGTAGCTAGTCCTCGAGTCATCGAAGCCATCTACTCTGAGTGTATCCCCGTCATTCTCTCGGTTAACTTCGTGTTACCATTCACTGATGTTCTTCGTTGGGAGACTTTCTCTGTACTGGTCGACGTTTCAGAGATTCCGAGGTTGAAGGAGATTCTCATGTCGATCTCCAATGAGAAATATGAATGGCTTAAAAGTAACTTGAGGTATGTGAGGAGACATTTCGAGCTTAACGATCCTCCTCAGAGATTTGATGCATTTCACTTGACTCTTCATTCCATTTGGTTACGTAGACTTAACTTGAAGCTTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

480

Amino Acids

55.97

Weight (kDa)

9.09

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 147 - 432 6.1e-60 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012970)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25310
fragaria_vesca FvH4_2g28831 FvH4_4g07691 FvH4_6g35481
malus_domestica MD15G1127300.v1.1
prunus_persica Prupe.1G483000_v2.0.a1
pyrus_communis pycom15g11470 pycom15g11480
rosa_chinensis RchiOBHm_Chr6g0297591
rosa_laevigata RLG00000011579
rosa_multiflora Rmu_ssc0000319.1_g000009
rosa_roxburghii Rroxscaffold_7G00170330
rosa_rugosa Rorug06G0274100
rosa_samantha Rh6AG384900 Rh6BG393000 Rh6CG398800 Rh6DG385400
rosa_wichuraiana Rw6G033530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 465, 1251, 1420
AccII CGCG 2 cut(s) 521, 955
AciI CCGC 6 cut(s) 485, 521, 945, 953, 955, 981
AclI AACGTT 1 cut(s) 165
AclWI GGATC 4 cut(s) 64, 226, 575, 1358
AcsI RAATTY 5 cut(s) 20, 122, 128, 295, 396
AfaI GTAC 2 cut(s) 574, 1245
AfiI CCNNNNNNNGG 2 cut(s) 965, 994
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 1 cut(s) 1035
AluBI AGCT 8 cut(s) 154, 344, 416, 593, 794, 1136, 1357, 1435
AluI AGCT 8 cut(s) 154, 344, 416, 593, 794, 1136, 1357, 1435
Alw26I GTCTC 2 cut(s) 1226, 1339
AlwI GGATC 4 cut(s) 64, 226, 575, 1358
Ama87I CYCGRG 2 cut(s) 965, 1143
AoxI GGCC 1 cut(s) 956
ApoI RAATTY 5 cut(s) 20, 122, 128, 295, 396
Asp700I GAANNNNTTC 1 cut(s) 1059
AspS9I GGNCC 3 cut(s) 500, 776, 992
AsuHPI GGTGA 2 cut(s) 197, 917
AsuII TTCGAA 2 cut(s) 157, 350
AvaI CYCGRG 2 cut(s) 965, 1143
AvaII GGWCC 3 cut(s) 500, 776, 992
BauI CACGAG 1 cut(s) 25
BccI CCATC 4 cut(s) 418, 491, 541, 1166
BcgI CGANNNNNNTGC 2 cut(s) 312, 346
BciT130I CCWGG 1 cut(s) 1037
BciVI GTATCC 1 cut(s) 1184
BclI TGATCA 2 cut(s) 745, 913
BcoDI GTCTC 2 cut(s) 1226, 1339
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 3 cut(s) 174, 488, 1137
BfuI GTATCC 1 cut(s) 1184
BisI GCNGC 1 cut(s) 956
BlpI GCTNAGC 1 cut(s) 663
BlsI GCNGC 1 cut(s) 957
Bme1390I CCNGG 1 cut(s) 1037
Bme18I GGWCC 3 cut(s) 500, 776, 992
BmeT110I CYCGRG 2 cut(s) 965, 1143
BmgT120I GGNCC 3 cut(s) 500, 776, 992
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 1 cut(s) 1037
BmrI ACTGGG 1 cut(s) 1113
BmsI GCATC 3 cut(s) 338, 649, 1372
BmuI ACTGGG 1 cut(s) 1113
BplI GAGNNNNNCTC 2 cut(s) 1223, 1255
Bpu1102I GCTNAGC 1 cut(s) 663
Bpu14I TTCGAA 2 cut(s) 157, 350
BpuEI CTTGAG 1 cut(s) 1351
Bsa29I ATCGAT 2 cut(s) 59, 332
BsaAI YACGTR 2 cut(s) 1418, 1440
BsaBI GATNNNNATC 1 cut(s) 703
BsaJI CCNNGG 4 cut(s) 953, 964, 988, 1268
BsaWI WCCGGW 4 cut(s) 182, 274, 288, 994
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 965, 994
Bse1I ACTGG 3 cut(s) 1025, 1119, 1251
Bse8I GATNNNNATC 1 cut(s) 703
BseBI CCWGG 1 cut(s) 1037
BseCI ATCGAT 2 cut(s) 59, 332
BseDI CCNNGG 4 cut(s) 953, 964, 988, 1268
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 1 cut(s) 703
BseLI CCNNNNNNNGG 2 cut(s) 965, 994
BseMII CTCAG 7 cut(s) 141, 405, 534, 876, 937, 1158, 1385
BseNI ACTGG 3 cut(s) 1025, 1119, 1251
BseRI GAGGAG 2 cut(s) 1357, 1359
Bsh1236I CGCG 2 cut(s) 521, 955
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 958
BshVI ATCGAT 2 cut(s) 59, 332
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 2 cut(s) 965, 1143
BsiSI CCGG 4 cut(s) 183, 275, 289, 995
BslFI GGGAC 2 cut(s) 789, 1101
BslI CCNNNNNNNGG 2 cut(s) 965, 994
BsmAI GTCTC 2 cut(s) 1226, 1339
BsmFI GGGAC 2 cut(s) 789, 1101
BsnI GGCC 1 cut(s) 958
BsoBI CYCGRG 2 cut(s) 965, 1143
Bsp119I TTCGAA 2 cut(s) 157, 350
Bsp1720I GCTNAGC 1 cut(s) 663
BspACI CCGC 6 cut(s) 485, 521, 945, 953, 955, 981
BspANI GGCC 1 cut(s) 958
BspCNI CTCAG 7 cut(s) 142, 404, 535, 877, 936, 1159, 1384
BspDI ATCGAT 2 cut(s) 59, 332
BspFNI CGCG 2 cut(s) 521, 955
BspHI TCATGA 1 cut(s) 1089
BspLI GGNNCC 1 cut(s) 777
BspPI GGATC 4 cut(s) 64, 226, 575, 1358
BspT104I TTCGAA 2 cut(s) 157, 350
BsrI ACTGG 3 cut(s) 1025, 1119, 1251
BssECI CCNNGG 4 cut(s) 953, 964, 988, 1268
BssNAI GTATAC 1 cut(s) 466
BssSI CACGAG 1 cut(s) 25
Bst1107I GTATAC 1 cut(s) 466
Bst2BI CACGAG 1 cut(s) 25
Bst2UI CCWGG 1 cut(s) 1037
Bst4CI ACNGT 3 cut(s) 241, 504, 992
Bst6I CTCTTC 3 cut(s) 440, 885, 1404
BstBAI YACGTR 2 cut(s) 1418, 1440
BstBI TTCGAA 2 cut(s) 157, 350
BstC8I GCNNGC 2 cut(s) 479, 979
BstDSI CCRYGG 2 cut(s) 953, 988
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 2 cut(s) 521, 955
BstMAI GTCTC 2 cut(s) 1226, 1339
BstMCI CGRYCG 1 cut(s) 63
BstNI CCWGG 1 cut(s) 1037
BstSCI CCNGG 1 cut(s) 1035
BstSNI TACGTA 2 cut(s) 1418, 1440
BstUI CGCG 2 cut(s) 521, 955
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BstZ17I GTATAC 1 cut(s) 466
Bsu15I ATCGAT 2 cut(s) 59, 332
BsuI GTATCC 1 cut(s) 1184
BsuRI GGCC 1 cut(s) 958
BsuTUI ATCGAT 2 cut(s) 59, 332
BtgI CCRYGG 2 cut(s) 953, 988
BtsCI GGATG 1 cut(s) 715
BtsIMutI CAGTG 4 cut(s) 237, 1018, 1126, 1212
Cac8I GCNNGC 2 cut(s) 479, 979
CciI TCATGA 1 cut(s) 1089
Cfr13I GGNCC 3 cut(s) 500, 776, 992
Cfr42I CCGCGG 1 cut(s) 956
ClaI ATCGAT 2 cut(s) 59, 332
CpoI CGGWCCG 1 cut(s) 992
Csp6I GTAC 2 cut(s) 573, 1244
CspI CGGWCCG 1 cut(s) 992
CviAII CATG 4 cut(s) 218, 754, 1090, 1288
CviQI GTAC 2 cut(s) 573, 1244
DraI TTTAAA 1 cut(s) 1014
Eam1104I CTCTTC 3 cut(s) 440, 885, 1404
EarI CTCTTC 3 cut(s) 440, 885, 1404
Eco105I TACGTA 2 cut(s) 1418, 1440
Eco47I GGWCC 3 cut(s) 500, 776, 992
Eco88I CYCGRG 2 cut(s) 965, 1143
EcoO109I RGGNCCY 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 1035
EcoT22I ATGCAT 1 cut(s) 1387
FaeI CATG 4 cut(s) 221, 757, 1093, 1291
FaqI GGGAC 2 cut(s) 789, 1101
FatI CATG 4 cut(s) 217, 753, 1089, 1287
FbaI TGATCA 2 cut(s) 745, 913
FblI GTMKAC 3 cut(s) 465, 1251, 1420
Fnu4HI GCNGC 1 cut(s) 956
FokI GGATG 1 cut(s) 702
Fsp4HI GCNGC 1 cut(s) 956
FspBI CTAG 3 cut(s) 174, 488, 1137
GluI GCNGC 1 cut(s) 956
HaeIII GGCC 1 cut(s) 958
HapII CCGG 4 cut(s) 183, 275, 289, 995
Hin1II CATG 4 cut(s) 221, 757, 1093, 1291
HincII GTYRAC 3 cut(s) 358, 1195, 1252
HindII GTYRAC 3 cut(s) 358, 1195, 1252
HindIII AAGCTT 3 cut(s) 342, 591, 1433
HpaI GTTAAC 2 cut(s) 358, 1195
HpaII CCGG 4 cut(s) 183, 275, 289, 995
HphI GGTGA 2 cut(s) 197, 917
Hpy188III TCNNGA 6 cut(s) 25, 43, 494, 767, 884, 1090
Hpy99I CGWCG 2 cut(s) 206, 1256
HpyAV CCTTC 7 cut(s) 467, 521, 552, 641, 845, 1070, 1270
HpyCH4III ACNGT 3 cut(s) 241, 504, 992
HpyCH4IV ACGT 4 cut(s) 165, 1254, 1417, 1439
HpyCH4V TGCA 1 cut(s) 1385
HpySE526I ACGT 4 cut(s) 165, 1254, 1417, 1439
Hsp92II CATG 4 cut(s) 221, 757, 1093, 1291
Ksp22I TGATCA 2 cut(s) 745, 913
KspAI GTTAAC 2 cut(s) 358, 1195
KspI CCGCGG 1 cut(s) 956
LweI GCATC 3 cut(s) 338, 649, 1372
MaeI CTAG 3 cut(s) 174, 488, 1137
MaeII ACGT 4 cut(s) 165, 1254, 1417, 1439
MaeIII GTNAC 8 cut(s) 428, 619, 764, 874, 1031, 1203, 1325, 1413
MflI RGATCY 1 cut(s) 231
MluCI AATT 7 cut(s) 20, 122, 128, 295, 396, 652, 841
MlyI GAGTC 4 cut(s) 207, 871, 1155, 1390
Mph1103I ATGCAT 1 cut(s) 1387
MroXI GAANNNNTTC 1 cut(s) 1059
MseI TTAA 9 cut(s) 357, 453, 858, 1013, 1073, 1194, 1320, 1359, 1425
MspA1I CMGCKG 1 cut(s) 955
MspI CCGG 4 cut(s) 183, 275, 289, 995
MspR9I CCNGG 1 cut(s) 1037
MvaI CCWGG 1 cut(s) 1037
MvnI CGCG 2 cut(s) 521, 955
NlaIII CATG 4 cut(s) 221, 757, 1093, 1291
NlaIV GGNNCC 1 cut(s) 777
NmuCI GTSAC 3 cut(s) 764, 874, 1031
NsiI ATGCAT 1 cut(s) 1387
NspV TTCGAA 2 cut(s) 157, 350
PaeR7I CTCGAG 1 cut(s) 1143
PagI TCATGA 1 cut(s) 1089
PcsI WCGNNNNNNNCGW 2 cut(s) 474, 1359
PdmI GAANNNNTTC 1 cut(s) 1059
PfeI GAWTC 9 cut(s) 30, 36, 209, 534, 727, 820, 1006, 1264, 1282
PkrI GCNGC 1 cut(s) 957
Ple19I CGATCG 1 cut(s) 63
PleI GAGTC 4 cut(s) 206, 871, 1154, 1390
PpsI GAGTC 4 cut(s) 206, 871, 1154, 1390
Ppu21I YACGTR 2 cut(s) 1418, 1440
PpuMI RGGWCCY 1 cut(s) 776
Psp1406I AACGTT 1 cut(s) 165
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 1 cut(s) 1035
PspGI CCWGG 1 cut(s) 1035
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 3 cut(s) 500, 776, 992
PspPPI RGGWCCY 1 cut(s) 776
PspXI VCTCGAGB 1 cut(s) 1143
PsuI RGATCY 1 cut(s) 231
PvuI CGATCG 1 cut(s) 63
RsaI GTAC 2 cut(s) 574, 1245
RsaNI GTAC 2 cut(s) 573, 1244
Rsr2I CGGWCCG 1 cut(s) 992
RsrII CGGWCCG 1 cut(s) 992
SacII CCGCGG 1 cut(s) 956
SalI GTCGAC 1 cut(s) 1250
SaqAI TTAA 9 cut(s) 357, 453, 858, 1013, 1073, 1194, 1320, 1359, 1425
SatI GCNGC 1 cut(s) 956
Sau96I GGNCC 3 cut(s) 500, 776, 992
SchI GAGTC 4 cut(s) 207, 871, 1155, 1390
ScrFI CCNGG 1 cut(s) 1037
SfaNI GCATC 3 cut(s) 338, 649, 1372
Sfr274I CTCGAG 1 cut(s) 1143
Sfr303I CCGCGG 1 cut(s) 956
SfuI TTCGAA 2 cut(s) 157, 350
SgrBI CCGCGG 1 cut(s) 956
SinI GGWCC 3 cut(s) 500, 776, 992
SlaI CTCGAG 1 cut(s) 1143
SmlI CTYRAG 2 cut(s) 1143, 1330
SmoI CTYRAG 2 cut(s) 1143, 1330
SnaBI TACGTA 2 cut(s) 1418, 1440
SpeI ACTAGT 1 cut(s) 173
Sse9I AATT 7 cut(s) 20, 122, 128, 295, 396, 652, 841
SsiI CCGC 6 cut(s) 485, 521, 945, 953, 955, 981
SspMI CTAG 3 cut(s) 174, 488, 1137
StyD4I CCNGG 1 cut(s) 1035
TaaI ACNGT 3 cut(s) 241, 504, 992
TaiI ACGT 4 cut(s) 168, 1257, 1420, 1442
TaqII GACCGA 1 cut(s) 1018
TasI AATT 7 cut(s) 20, 122, 128, 295, 396, 652, 841
TatI WGTACW 1 cut(s) 1243
TauI GCSGC 1 cut(s) 958
TfiI GAWTC 9 cut(s) 30, 36, 209, 534, 727, 820, 1006, 1264, 1282
Tru1I TTAA 9 cut(s) 357, 453, 858, 1013, 1073, 1194, 1320, 1359, 1425
Tru9I TTAA 9 cut(s) 357, 453, 858, 1013, 1073, 1194, 1320, 1359, 1425
TscAI CASTG 4 cut(s) 244, 1025, 1126, 1219
TseFI GTSAC 3 cut(s) 764, 874, 1031
Tsp45I GTSAC 3 cut(s) 764, 874, 1031
TspDTI ATGAA 8 cut(s) 28, 526, 742, 834, 1068, 1078, 1326, 1391
TspRI CASTG 4 cut(s) 244, 1025, 1126, 1219
VpaK11BI GGWCC 3 cut(s) 500, 776, 992
XapI RAATTY 5 cut(s) 20, 122, 128, 295, 396
XhoI CTCGAG 1 cut(s) 1143
XmiI GTMKAC 3 cut(s) 465, 1251, 1420
XmnI GAANNNNTTC 1 cut(s) 1059
XspI CTAG 3 cut(s) 174, 488, 1137
Zsp2I ATGCAT 1 cut(s) 1387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.