AT5G25840

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015896
Physical Location & Seq
Reverse (-)
9010643 .. 9011618
976 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25840.1

Sequence Viewer

Length: 522 bp
ATGGCCCCCCTTCAACGCACCATCTCGGATATTTCACACCAAATTTCAATAGACAACACTCTAACAAAAGAACCATCTCCAACCGCAACCGCAACAACTTTGTTATCCTTATCAGCGATATCCGAAGTGGAAGATGCAAAGTGTGAGTGTTGTGGCATGTCTGAAGAGTGCACGCCGGAGTACATACACCGTGTGCGTTCAAAATTCTCAGGGAAGCTGATTTGCGGGCTGTGCGAAAAAGCGGTGGAGGGGGAGATGGAGAAGATGAACAACAGTGAAGTGCTGATGGAGAAGCGGCGAGAAGAGGCAGTGAAGGTACACATGAGCGCATGTTCCCGGTTCAACAGGCTAGGCCGGAGTTATCCAGTGTTGTACCAAGCAGAAGCGGTTAAGGAAATGCTGAAAAAGAGGTCTAAGAAGATGGTTGGAGCCACCAAGCCTGAAAAAGGTGGCCTCGCTAGGAGCTCTAGCTGCATGCCGGCTTTAGCTAAGGAGCTTAAAGATCGTACCTTGGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

173

Amino Acids

19.18

Weight (kDa)

8.76

Isoelectric Point (pI)

72.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 43 - 140 9e-32 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25840
fragaria_vesca FvH4_2g35390
malus_domestica MD08G1112600.v1.1 MD15G1091900.v1.1
prunus_persica Prupe.1G446800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306521
rosa_laevigata RLG00000010790
rosa_multiflora Rmu_sc0002971.1_g000007
rosa_roxburghii Rroxscaffold_7G00161700
rosa_rugosa Rorug06G0350100
rosa_samantha Rh6AG462200 Rh6BG438500 Rh6CG476500 Rh6DG463200
rosa_wichuraiana Rw6G040290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 84, 90, 225, 242, 295, 386
AcsI RAATTY 2 cut(s) 42, 203
AcuI CTGAAG 1 cut(s) 183
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 4 cut(s) 182, 318, 374, 508
AfiI CCNNNNNNNGG 1 cut(s) 446
AgsI TTSAA 4 cut(s) 14, 48, 201, 343
AluBI AGCT 5 cut(s) 217, 465, 471, 488, 496
AluI AGCT 5 cut(s) 217, 465, 471, 488, 496
Alw21I GWGCWC 2 cut(s) 173, 467
Alw44I GTGCAC 1 cut(s) 169
AoxI GGCC 3 cut(s) 3, 352, 451
ApaLI GTGCAC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 471
ApoI RAATTY 2 cut(s) 42, 203
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 467
Bbv12I GWGCWC 2 cut(s) 173, 467
BbvI GCAGC 1 cut(s) 458
BccI CCATC 5 cut(s) 29, 82, 250, 280, 415
BcnI CCSGG 1 cut(s) 337
BfaI CTAG 3 cut(s) 350, 459, 468
BisI GCNGC 2 cut(s) 296, 472
BlsI GCNGC 2 cut(s) 297, 473
Bme1390I CCNGG 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 430
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 510
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 510
BseLI CCNNNNNNNGG 1 cut(s) 446
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 365
BseSI GKGCMC 1 cut(s) 173
BseXI GCAGC 1 cut(s) 458
BshFI GGCC 3 cut(s) 5, 354, 453
BsiHKAI GWGCWC 2 cut(s) 173, 467
BsiSI CCGG 4 cut(s) 176, 337, 355, 479
BslI CCNNNNNNNGG 1 cut(s) 446
BsnI GGCC 3 cut(s) 5, 354, 453
Bsp1286I GDGCHC 2 cut(s) 173, 467
Bsp143I GATC 1 cut(s) 502
BspACI CCGC 6 cut(s) 84, 90, 225, 242, 295, 386
BspANI GGCC 3 cut(s) 5, 354, 453
BspCNI CTCAG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 6, 430
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 1 cut(s) 502
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 2 cut(s) 191, 275
Bst6I CTCTTC 2 cut(s) 159, 297
BstC8I GCNNGC 4 cut(s) 173, 227, 476, 480
BstDEI CTNAG 3 cut(s) 208, 414, 489
BstENI CCTNNNNNAGG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 1 cut(s) 505
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 2 cut(s) 231, 471
BstNSI RCATGY 3 cut(s) 160, 333, 478
BstSCI CCNGG 1 cut(s) 335
BstSLI GKGCMC 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 458
BsuRI GGCC 3 cut(s) 5, 354, 453
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 3 cut(s) 280, 315, 372
Cac8I GCNNGC 4 cut(s) 173, 227, 476, 480
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 4 cut(s) 181, 317, 373, 507
CviAII CATG 4 cut(s) 157, 322, 330, 475
CviQI GTAC 4 cut(s) 181, 317, 373, 507
DdeI CTNAG 3 cut(s) 208, 414, 489
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
DraIII CACNNNGTG 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 159, 297
EarI CTCTTC 2 cut(s) 159, 297
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 1 cut(s) 467
Eco32I GATATC 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 465
Eco57I CTGAAG 1 cut(s) 183
EcoICRI GAGCTC 1 cut(s) 465
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 160, 325, 333, 478
FaiI YATR 5 cut(s) 158, 185, 323, 331, 476
FatI CATG 4 cut(s) 156, 321, 329, 474
FauI CCCGC 1 cut(s) 218
Fnu4HI GCNGC 2 cut(s) 296, 472
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 2 cut(s) 296, 472
FspBI CTAG 3 cut(s) 350, 459, 468
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 296, 472
HaeIII GGCC 3 cut(s) 5, 354, 453
HapII CCGG 4 cut(s) 176, 337, 355, 479
HhaI GCGC 1 cut(s) 329
Hin1II CATG 4 cut(s) 160, 325, 333, 478
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HpaII CCGG 4 cut(s) 176, 337, 355, 479
Hpy166II GTNNAC 2 cut(s) 171, 319
Hpy188I TCNGA 3 cut(s) 28, 124, 163
Hpy8I GTNNAC 2 cut(s) 171, 319
HpyAV CCTTC 2 cut(s) 20, 307
HpyCH4III ACNGT 2 cut(s) 191, 275
HpyCH4V TGCA 3 cut(s) 137, 171, 474
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 471
HpyF3I CTNAG 3 cut(s) 208, 414, 489
Hsp92II CATG 4 cut(s) 160, 325, 333, 478
HspAI GCGC 1 cut(s) 327
KroI GCCGGC 1 cut(s) 478
KroNI GCCGGC 1 cut(s) 480
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 3 cut(s) 428, 462, 493
LpnPI CCDG 8 cut(s) 189, 195, 331, 350, 368, 378, 453, 492
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 1 cut(s) 124
MaeI CTAG 3 cut(s) 350, 459, 468
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 5 cut(s) 143, 176, 274, 314, 430
MhlI GDGCHC 2 cut(s) 173, 467
MluCI AATT 3 cut(s) 42, 203, 517
MmeI TCCRAC 2 cut(s) 104, 406
MnlI CCTC 4 cut(s) 241, 298, 402, 464
MroNI GCCGGC 1 cut(s) 478
MseI TTAA 3 cut(s) 390, 498, 516
MspI CCGG 4 cut(s) 176, 337, 355, 479
MspR9I CCNGG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 231, 471
NaeI GCCGGC 1 cut(s) 480
NciI CCSGG 1 cut(s) 337
NdeII GATC 1 cut(s) 502
NgoMIV GCCGGC 1 cut(s) 478
NlaIII CATG 4 cut(s) 160, 325, 333, 478
NlaIV GGNNCC 2 cut(s) 6, 430
NspI RCATGY 3 cut(s) 160, 333, 478
PaeI GCATGC 1 cut(s) 478
PdiI GCCGGC 1 cut(s) 480
PkrI GCNGC 2 cut(s) 297, 473
Psp124BI GAGCTC 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 6, 430
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 4 cut(s) 182, 318, 374, 508
RsaNI GTAC 4 cut(s) 181, 317, 373, 507
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 3 cut(s) 390, 498, 516
SatI GCNGC 2 cut(s) 296, 472
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 2 cut(s) 173, 467
SetI ASST 9 cut(s) 219, 318, 413, 451, 467, 473, 490, 498, 512
SfaNI GCATC 1 cut(s) 124
SphI GCATGC 1 cut(s) 478
Sse9I AATT 3 cut(s) 42, 203, 517
SsiI CCGC 6 cut(s) 84, 90, 225, 242, 295, 386
SspMI CTAG 3 cut(s) 350, 459, 468
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 191, 275
TasI AATT 3 cut(s) 42, 203, 517
TatI WGTACW 1 cut(s) 180
TauI GCSGC 1 cut(s) 298
Tru1I TTAA 3 cut(s) 390, 498, 516
Tru9I TTAA 3 cut(s) 390, 498, 516
TscAI CASTG 3 cut(s) 280, 315, 372
TseI GCWGC 1 cut(s) 471
TspDTI ATGAA 1 cut(s) 281
TspRI CASTG 3 cut(s) 280, 315, 372
VneI GTGCAC 1 cut(s) 169
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 2 cut(s) 42, 203
XceI RCATGY 3 cut(s) 160, 333, 478
XspI CTAG 3 cut(s) 350, 459, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.