RchiOBHm_Chr6g0306521

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015896
Physical Location & Seq
Forward (+)
65192961 .. 65193662
702 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27554

Sequence Viewer

Length: 615 bp
ATGGCCCCAAACAGAGAAAACCACATGGGTTCCTACTCCAAGAGCAAGCCACCAACACTATCAATGGGAGGTAGTACTATACAAAGAACCAAATCCGACATTTCGATAGAAGTTCTGAGCAAAGAGTTAGCAGCCACCGACATTCATGAAGACGACGAGAAGCTGCTGCCGCCCATATCAGAAGTCGAAGAGGCCAAATGCGAGTGCTGTGGCATGTCCGAGGAGTGCACGGCCGAGTACATAAACCGCGTGCGAAACCAGTTTTCAGGGAAGCTGATTTGCGGGCTGTGCGCTGAAGCTGTGAATGGAGAGATGGACAAGAACGGAGGGAAGAGGGAAGAGGCTGTGAATGAGCATATGAGTGCTTGTGTGAAGTTTAATAGGCTGGAGAGGACAAACCCTGTTCTGTACCAAGCTGAGGCCATCAAAGAGATTTTGAGGAAGAGTGGTTCTACGAGTACTAGCAGGTCCAAATCTATGAGTTACAAGAGTGGTGTTCAGAAGAACAATGGTGGCCTGGCGAGGAGCTCCAGTTGTATTCCTGCTATCACCCCGAAGAAGTTGGTGAGCCGCTACACTAGTAAAACCGAGCTAATAGAATTACGTCTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

204

Amino Acids

22.46

Weight (kDa)

8.07

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 62 - 150 2.8e-34 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25840
fragaria_vesca FvH4_2g35390
malus_domestica MD08G1112600.v1.1 MD15G1091900.v1.1
prunus_persica Prupe.1G446800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306521
rosa_laevigata RLG00000010790
rosa_multiflora Rmu_sc0002971.1_g000007
rosa_roxburghii Rroxscaffold_7G00161700
rosa_rugosa Rorug06G0350100
rosa_samantha Rh6AG462200 Rh6BG438500 Rh6CG476500 Rh6DG463200
rosa_wichuraiana Rw6G040290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccII CGCG 1 cut(s) 249
AciI CCGC 4 cut(s) 170, 247, 282, 571
AcoI YGGCCR 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 4 cut(s) 76, 239, 410, 460
AfiI CCNNNNNNNGG 1 cut(s) 418
AhlI ACTAGT 1 cut(s) 578
AjnI CCWGG 1 cut(s) 516
AloI GAACNNNNNNTCC 2 cut(s) 433, 465
AluBI AGCT 6 cut(s) 163, 274, 299, 416, 528, 592
AluI AGCT 6 cut(s) 163, 274, 299, 416, 528, 592
Alw21I GWGCWC 2 cut(s) 230, 530
Alw44I GTGCAC 1 cut(s) 226
AoxI GGCC 5 cut(s) 3, 192, 231, 420, 514
ApaLI GTGCAC 1 cut(s) 226
ApeKI GCWGC 3 cut(s) 131, 163, 166
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 4, 468
AsuHPI GGTGA 2 cut(s) 541, 577
AvaII GGWCC 1 cut(s) 468
BaeGI GKGCMC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 230, 530
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 3 cut(s) 143, 150, 153
BccI CCATC 2 cut(s) 307, 431
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 1 cut(s) 518
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 2 cut(s) 462, 579
BfuAI ACCTGC 1 cut(s) 456
BisI GCNGC 5 cut(s) 132, 164, 167, 170, 571
BlsI GCNGC 5 cut(s) 133, 165, 168, 171, 572
BmcAI AGTACT 2 cut(s) 76, 460
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 468
BmgT120I GGNCC 2 cut(s) 4, 468
BmiI GGNNCC 2 cut(s) 6, 31
BmrFI CCNGG 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 407, 514
Bpu10I CCTNAGC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 259, 531
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 107, 408
BseNI ACTGG 2 cut(s) 259, 531
BseRI GAGGAG 2 cut(s) 236, 538
BseSI GKGCMC 1 cut(s) 230
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 143, 150, 153
Bsh1236I CGCG 1 cut(s) 249
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 5 cut(s) 5, 194, 233, 422, 516
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 230, 530
BslI CCNNNNNNNGG 1 cut(s) 418
BsnI GGCC 5 cut(s) 5, 194, 233, 422, 516
Bsp1286I GDGCHC 2 cut(s) 230, 530
BspACI CCGC 4 cut(s) 170, 247, 282, 571
BspANI GGCC 5 cut(s) 5, 194, 233, 422, 516
BspCNI CTCAG 2 cut(s) 108, 409
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 6, 31
BspMI ACCTGC 1 cut(s) 456
BsrI ACTGG 2 cut(s) 259, 531
BssECI CCNNGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 518
Bst6I CTCTTC 4 cut(s) 183, 326, 333, 437
BstC8I GCNNGC 3 cut(s) 47, 251, 284
BstDEI CTNAG 2 cut(s) 116, 417
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 1 cut(s) 293
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 2 cut(s) 169, 288
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 1 cut(s) 516
BstSLI GKGCMC 1 cut(s) 230
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 143, 150, 153
BstV2I GAAGAC 1 cut(s) 156
BstZI CGGCCG 1 cut(s) 231
BsuRI GGCC 5 cut(s) 5, 194, 233, 422, 516
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 3 cut(s) 47, 251, 284
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 4, 468
Csp6I GTAC 4 cut(s) 75, 238, 409, 459
CviAII CATG 3 cut(s) 25, 146, 214
CviQI GTAC 4 cut(s) 75, 238, 409, 459
DdeI CTNAG 2 cut(s) 116, 417
EaeI YGGCCR 1 cut(s) 231
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 4 cut(s) 183, 326, 333, 437
EarI CTCTTC 4 cut(s) 183, 326, 333, 437
Ecl136II GAGCTC 1 cut(s) 528
EclXI CGGCCG 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 468
Eco52I CGGCCG 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 528
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 530
FaeI CATG 3 cut(s) 28, 149, 217
FaiI YATR 9 cut(s) 26, 80, 147, 176, 215, 242, 357, 359, 479
FatI CATG 3 cut(s) 24, 145, 213
FauI CCCGC 1 cut(s) 275
FauNDI CATATG 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 132, 164, 167, 170, 571
FriOI GRGCYC 1 cut(s) 530
Fsp4HI GCNGC 5 cut(s) 132, 164, 167, 170, 571
FspBI CTAG 2 cut(s) 462, 579
GlaI GCGC 1 cut(s) 292
GluI GCNGC 5 cut(s) 132, 164, 167, 170, 571
GsuI CTGGAG 2 cut(s) 407, 514
HaeIII GGCC 5 cut(s) 5, 194, 233, 422, 516
HhaI GCGC 1 cut(s) 293
Hin1II CATG 3 cut(s) 28, 149, 217
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HphI GGTGA 2 cut(s) 541, 577
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 5 cut(s) 97, 117, 181, 220, 501
Hpy188III TCNNGA 2 cut(s) 146, 608
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 604
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 169, 288
HpyF3I CTNAG 2 cut(s) 116, 417
HpySE526I ACGT 1 cut(s) 604
Hsp92II CATG 3 cut(s) 28, 149, 217
HspAI GCGC 1 cut(s) 291
LmnI GCTCC 2 cut(s) 525, 533
Lsp1109I GCAGC 3 cut(s) 143, 150, 153
MaeI CTAG 2 cut(s) 462, 579
MaeII ACGT 1 cut(s) 604
MaeIII GTNAC 1 cut(s) 482
MboII GAAGA 7 cut(s) 161, 200, 343, 350, 454, 514, 568
MhlI GDGCHC 2 cut(s) 230, 530
MluCI AATT 1 cut(s) 599
MmeI TCCRAC 1 cut(s) 120
MseI TTAA 1 cut(s) 378
MslI CAYNNNNRTG 1 cut(s) 360
MspR9I CCNGG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 169, 288
NdeI CATATG 1 cut(s) 357
NlaIII CATG 3 cut(s) 28, 149, 217
NlaIV GGNNCC 2 cut(s) 6, 31
NmeAIII GCCGAG 1 cut(s) 259
NspI RCATGY 1 cut(s) 217
PagI TCATGA 1 cut(s) 145
PkrI GCNGC 5 cut(s) 133, 165, 168, 171, 572
Psp124BI GAGCTC 1 cut(s) 530
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 6, 31
PspPI GGNCC 2 cut(s) 4, 468
RsaI GTAC 4 cut(s) 76, 239, 410, 460
RsaNI GTAC 4 cut(s) 75, 238, 409, 459
RseI CAYNNNNRTG 1 cut(s) 360
SacI GAGCTC 1 cut(s) 530
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 5 cut(s) 132, 164, 167, 170, 571
Sau96I GGNCC 2 cut(s) 4, 468
ScaI AGTACT 2 cut(s) 76, 460
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 2 cut(s) 230, 530
SetI ASST 9 cut(s) 73, 165, 276, 301, 418, 470, 530, 594, 607
SinI GGWCC 1 cut(s) 468
SmiMI CAYNNNNRTG 1 cut(s) 360
SpeI ACTAGT 1 cut(s) 578
Sse9I AATT 1 cut(s) 599
SsiI CCGC 4 cut(s) 170, 247, 282, 571
SspMI CTAG 2 cut(s) 462, 579
SstI GAGCTC 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 516
TaiI ACGT 1 cut(s) 607
TaqI TCGA 2 cut(s) 104, 186
TasI AATT 1 cut(s) 599
TatI WGTACW 3 cut(s) 74, 237, 458
TauI GCSGC 2 cut(s) 172, 573
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseI GCWGC 3 cut(s) 131, 163, 166
TspDTI ATGAA 2 cut(s) 134, 162
TspGWI ACGGA 1 cut(s) 339
VneI GTGCAC 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 468
XceI RCATGY 1 cut(s) 217
XspI CTAG 2 cut(s) 462, 579
ZrmI AGTACT 2 cut(s) 76, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.