MD08G1112600.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015896
Physical Location & Seq
Forward (+)
9967678 .. 9968229
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1112600.v1.1.491

Sequence Viewer

Length: 552 bp
ATGCCACCAAACAGAGAAAACCAGATGGGTTCATACTCTAAGGCCAACATTGTCCACAAGCCACCAAGACTCTCAATGGGAAGTCTCCAAAGAACCATACCTGACATCTCAGCTGAACTAAGCAGCAGCATCAGCAGCAATGAAGCCATTGACGATGACATTCATCTTCCAACTATATCTGAGGTTGAGGATGCCAAGTGTGAGTGCTGTGGCATGTCCGAGGAGTGCACGAACGAGTACATAGATAGTGTGCGCAGCCAGTTTTCGGGGAAGTTAATTTGTGGGTTGTGTGCTGAGGCTGTGAAGGAAGAGATGCACAAGAATGGAGGCAAACGAGAAGCGGCTGTGAATCAGCATATGAGTTCTTGTGAAAAGTTCAATAGGCTTGGTAGGGCATACCCCGTTTTGTACCAAGCTGAGGCCATCAGAGAGATTTTCCAAAAGAGTTCAAGAATTAGGGCAAAATCCATGAGTCCTAGAGATGAGAGTGTTCGAAAGAGTAACGGGGGTATTGCAAGGAGCTCTAGTTGTATTTCCGTAAGAGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

184

Amino Acids

20.22

Weight (kDa)

7.56

Isoelectric Point (pI)

66.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 62 - 151 6.1e-34 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25840
fragaria_vesca FvH4_2g35390
malus_domestica MD08G1112600.v1.1 MD15G1091900.v1.1
prunus_persica Prupe.1G446800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306521
rosa_laevigata RLG00000010790
rosa_multiflora Rmu_sc0002971.1_g000007
rosa_roxburghii Rroxscaffold_7G00161700
rosa_rugosa Rorug06G0350100
rosa_samantha Rh6AG462200 Rh6BG438500 Rh6CG476500 Rh6DG463200
rosa_wichuraiana Rw6G040290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 254
AciI CCGC 1 cut(s) 341
AfaI GTAC 2 cut(s) 239, 410
AfiI CCNNNNNNNGG 2 cut(s) 265, 418
AgsI TTSAA 2 cut(s) 379, 450
AluBI AGCT 3 cut(s) 113, 416, 522
AluI AGCT 3 cut(s) 113, 416, 522
Alw21I GWGCWC 2 cut(s) 230, 524
Alw26I GTCTC 1 cut(s) 89
Alw44I GTGCAC 1 cut(s) 226
AoxI GGCC 2 cut(s) 42, 420
ApaLI GTGCAC 1 cut(s) 226
ApeKI GCWGC 4 cut(s) 123, 126, 135, 255
AspLEI GCGC 1 cut(s) 255
AsuII TTCGAA 1 cut(s) 493
BaeGI GKGCMC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 524
Bbv12I GWGCWC 2 cut(s) 230, 524
BbvCI CCTCAGC 2 cut(s) 294, 417
BbvI GCAGC 4 cut(s) 135, 138, 147, 267
BccI CCATC 2 cut(s) 19, 431
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 2 cut(s) 477, 525
BisI GCNGC 5 cut(s) 124, 127, 136, 256, 342
BlsI GCNGC 5 cut(s) 125, 128, 137, 257, 343
BmsI GCATC 3 cut(s) 138, 181, 303
Bpu10I CCTNAGC 2 cut(s) 294, 417
Bpu14I TTCGAA 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 418
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 2 cut(s) 265, 418
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 4 cut(s) 123, 171, 285, 408
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 4 cut(s) 135, 138, 147, 267
BshFI GGCC 2 cut(s) 44, 422
BsiHKAI GWGCWC 2 cut(s) 230, 524
BslI CCNNNNNNNGG 2 cut(s) 265, 418
BsmAI GTCTC 1 cut(s) 89
BsnI GGCC 2 cut(s) 44, 422
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 230, 524
BspACI CCGC 1 cut(s) 341
BspANI GGCC 2 cut(s) 44, 422
BspCNI CTCAG 4 cut(s) 122, 172, 286, 409
BspT104I TTCGAA 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 219
Bst6I CTCTTC 1 cut(s) 303
BstBI TTCGAA 1 cut(s) 493
BstDEI CTNAG 6 cut(s) 39, 109, 119, 180, 294, 417
BstF5I GGATG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 255
BstMAI GTCTC 1 cut(s) 89
BstMWI GCNNNNNNNGC 2 cut(s) 132, 135
BstNSI RCATGY 1 cut(s) 217
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 4 cut(s) 135, 138, 147, 267
BsuRI GGCC 2 cut(s) 44, 422
BtsCI GGATG 1 cut(s) 196
CfoI GCGC 1 cut(s) 255
Csp6I GTAC 2 cut(s) 238, 409
CviAII CATG 2 cut(s) 214, 469
CviQI GTAC 2 cut(s) 238, 409
DdeI CTNAG 6 cut(s) 39, 109, 119, 180, 294, 417
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 524
Eco53kI GAGCTC 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 524
FaeI CATG 2 cut(s) 217, 472
FaiI YATR 9 cut(s) 34, 98, 176, 215, 242, 357, 359, 397, 470
FatI CATG 2 cut(s) 213, 468
FauNDI CATATG 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 124, 127, 136, 256, 342
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 5 cut(s) 124, 127, 136, 256, 342
FspBI CTAG 2 cut(s) 477, 525
FspI TGCGCA 1 cut(s) 254
GlaI GCGC 1 cut(s) 254
GluI GCNGC 5 cut(s) 124, 127, 136, 256, 342
HaeIII GGCC 2 cut(s) 44, 422
HhaI GCGC 1 cut(s) 255
Hin1II CATG 2 cut(s) 217, 472
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 3 cut(s) 69, 349, 472
Hpy166II GTNNAC 2 cut(s) 55, 228
Hpy188I TCNGA 3 cut(s) 181, 220, 428
Hpy188III TCNNGA 1 cut(s) 450
Hpy8I GTNNAC 2 cut(s) 55, 228
HpyAV CCTTC 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 228, 316, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 135
HpyF3I CTNAG 6 cut(s) 39, 109, 119, 180, 294, 417
Hsp92II CATG 2 cut(s) 217, 472
HspAI GCGC 1 cut(s) 253
LmnI GCTCC 1 cut(s) 519
LpnPI CCDG 3 cut(s) 35, 114, 272
Lsp1109I GCAGC 4 cut(s) 135, 138, 147, 267
LweI GCATC 3 cut(s) 138, 181, 303
MaeI CTAG 2 cut(s) 477, 525
MaeIII GTNAC 1 cut(s) 500
MboII GAAGA 2 cut(s) 158, 320
MhlI GDGCHC 2 cut(s) 230, 524
MluCI AATT 3 cut(s) 276, 453, 547
MlyI GAGTC 2 cut(s) 63, 481
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 6 cut(s) 175, 181, 214, 289, 320, 412
MseI TTAA 2 cut(s) 275, 546
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 132, 135
NdeI CATATG 1 cut(s) 357
NlaIII CATG 2 cut(s) 217, 472
NsbI TGCGCA 1 cut(s) 254
NspI RCATGY 1 cut(s) 217
NspV TTCGAA 1 cut(s) 493
PfeI GAWTC 1 cut(s) 349
PkrI GCNGC 5 cut(s) 125, 128, 137, 257, 343
PleI GAGTC 2 cut(s) 63, 480
PpsI GAGTC 2 cut(s) 63, 480
Psp124BI GAGCTC 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 2 cut(s) 239, 410
RsaNI GTAC 2 cut(s) 238, 409
RseI CAYNNNNRTG 1 cut(s) 321
SacI GAGCTC 1 cut(s) 524
SaqAI TTAA 2 cut(s) 275, 546
SatI GCNGC 5 cut(s) 124, 127, 136, 256, 342
SchI GAGTC 2 cut(s) 63, 481
SduI GDGCHC 2 cut(s) 230, 524
SetI ASST 5 cut(s) 103, 115, 186, 418, 524
SfaNI GCATC 3 cut(s) 138, 181, 303
SfuI TTCGAA 1 cut(s) 493
SmiMI CAYNNNNRTG 1 cut(s) 321
Sse9I AATT 3 cut(s) 276, 453, 547
SsiI CCGC 1 cut(s) 341
SspMI CTAG 2 cut(s) 477, 525
SstI GAGCTC 1 cut(s) 524
TaqI TCGA 1 cut(s) 493
TasI AATT 3 cut(s) 276, 453, 547
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 2 cut(s) 275, 546
Tru9I TTAA 2 cut(s) 275, 546
TseI GCWGC 4 cut(s) 123, 126, 135, 255
TspDTI ATGAA 3 cut(s) 21, 152, 156
TspGWI ACGGA 1 cut(s) 526
VneI GTGCAC 1 cut(s) 226
XceI RCATGY 1 cut(s) 217
XspI CTAG 2 cut(s) 477, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.