AT5G27540

mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009905
Physical Location & Seq
Forward (+)
9722416 .. 9727454
5039 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27540.1

Sequence Viewer

Length: 1947 bp
ATGGCGAGATACGCTGCTGGTGCGGTTGATTGTCCTGGTTCTCCCAAATCTGTGCGAATCGTTGTGGTTGGTGATAAGGGCACTGGAAAATCAAGCTTGATTGTTGCTGCAGCCACTGATTCTTTCCCTCCCAATGTCCCTCCTGTTTTGCCTGATTACAAGTTGCCTATTGAATTCTTCCCTGATGGTATACCCGTCACCATTGTCGACACTTCCTCGAGGCCAGAAGATAGGGACATTGTTGCTGAGGAATTGAAGCGCGCAGATGCAGTAGTTTTGACATATGCCTGTGATCGACCTGAGACTCTCGAACGTTTGAGTGAATACTGGCTTCCAGAGCTTCGGCGATTAGAGGTAAAAATTCCTATTATCGTAGCAGGATGTAAGCTTGACTTCAGAGACGACAATAACCAGGTCAGCCTCGAACAAGTGATGTCACCTATAATGCAGCAGTTTCGGGAGATAGAGACTTGTATCGAGTGTTCTGCCCTGAAGCAACTCCAGGCACAAGAAGTTTTCTATTATGCACAAAAGACTGTCCTTCACCCAACAGGACCTCTGTTTGATCAAGATTCCCAGGCACTGAAACCCCGGTGTGTAAGGGCATTGAAGCGTATCTTTATACTCTGTGATCATGACAGAGATGGTGCTCTTAGCGAGGCTGAGTTAAATGATTTTCAGGTCAAGTGTTTCCATGCACCATTGCAGCCTTCTGAAATTGAAGGTGTTAAGAGGGTTGTGCAAGAAAAGTTGCCAGAAGGTGTGAATGAAAGAGGACTGACAGTCACTGGCTTCCTATTTTTACATGCACTTTTTATTGAGAAAGGGAGGCTTGAGACTACGTGGACTGTACTCAGGAAGTTTGGGTATAATAATGATATAAGACTTGCTGAAGAATTGCTTCCATCTGCAATATTCAAGCGAGCTCCTGATCAGAGCTTTGAGTTGACAAATGCAGCAATCGATTTCCTGAAAGGAATGTATATGTTGTTTGATGACGACCAGGATAACAATCTGAGACCTCAAGAGATCGAGGATCTTTTTTCAACTGCACCTGAAAGTCCATGGAAGGAAGCTCCTTATGAAGATGCTGCGGAAAAAACTGCCCTTGGAGGACTATCATTTGACGCTTTCCTGTCAATGTGGTCACTGATGACACTATTAGAACCGGCTCGGAGTGTGGAGAATTTGATTTACATTGGATTCCCGGGTGATCCATCGACTGCCATTCGTGTTACTAGGAGAAGACGTTTAGATCGCAAAAAGCAGCAATGTGAACGAAAAGTTTTCCAGTGCTTTGTTTTTGGACCTAATAATGCTGGAAAATCTGCATTGCTGAATTGCTTTCTTGGAAGGTCATATACTGATAACCAGGAATCAACTACTGATGAGCGTTATGCAGTAAATATGGTCGATGAATCGGGTGCTAAGAAAACTCTTATCATGAGGGAAATTCCAGAAGATGGAGTTCAAGGGCTATTTTCATCCAAGGAATCCTTAGCTGCATGTGACATAGCTGTCTTCGTGTATGACAGTTCTGATGAGTCGTCATGGAAGAGAGCGACCCAGTTGCTTGTAGAAGTTGCAAATTATGGTGAGGCCACTGGGTATGAGGTCCCTTGCCTCATGGTTTCAGCAAAAGACGATCTTGATTCATCTCCTATTTCCATACAAGAATCCACCAGGATGACACAAGATATGGGAATAGAGCCTCCGGTATCAATCAGCTCCAAATTGGGGGATTTCAACAATTTATTCCGCAAAATCTTAACTGCAGCACAACATCCTCATTTGAGCATTCCAGAGACCGAAGCAGGCAAAAGCCGCAAACATTACAACAGGCTAATCAACCGCTCTCTTATGGCCGTTTCCATTGGTGCTGCTGCTGTGGTCGTTGGGCTGGCTGCATACCGTGTGTATGCCACAAGAAAGAGCTCGTCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006810 GO:0006839 GO:0006915 GO:0006928 GO:0006996 GO:0007005 GO:0007006 GO:0007017 GO:0007018 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008219 GO:0008637 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010154 GO:0010970 GO:0012501 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0019725 GO:0019867 GO:0022414 GO:0023052 GO:0030154 GO:0030705 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032989 GO:0034643 GO:0035794 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044706 GO:0046902 GO:0046907 GO:0047497 GO:0048316 GO:0048468 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0051704 GO:0051716 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0072384 GO:0090559 GO:0097190 GO:0097345 GO:0098573 GO:0098588 GO:0098805 GO:0099111 GO:1902108 GO:1902110 GO:1902686 GO:1905710
KEGG Pathways
Metabolic & Signaling
ko04137 ko04214 map04137 map04214
Pfam Domains
Protein Families
EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

648

Amino Acids

72.32

Weight (kDa)

5.17

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 19 - 180 1.5e-15 Ras family
Roc PF08477 19 - 131 2.8e-06 Ras of Complex, Roc, domain of DAPkinase
EF_assoc_2 PF08356 234 - 319 2.8e-30 EF hand associated
EF_assoc_1 PF08355 356 - 424 1.6e-21 EF hand associated
Ras PF00071 433 - 589 4.7e-08 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1517
AbsI CCTCGAGG 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 1463
AccBSI CCGCTC 1 cut(s) 1854
AccI GTMKAC 2 cut(s) 190, 207
AccII CGCG 1 cut(s) 261
AciI CCGC 5 cut(s) 23, 1094, 1759, 1825, 1852
AclI AACGTT 1 cut(s) 313
AclWI GGATC 2 cut(s) 1044, 1208
AcoI YGGCCR 1 cut(s) 1863
AcsI RAATTY 4 cut(s) 173, 360, 1186, 1452
AcuI CTGAAG 3 cut(s) 379, 512, 912
AfaI GTAC 1 cut(s) 852
AfiI CCNNNNNNNGG 2 cut(s) 1463, 1737
AgsI TTSAA 8 cut(s) 173, 256, 610, 722, 919, 1047, 1472, 1747
AhdI GACNNNNNGTC 1 cut(s) 782
AjnI CCWGG 7 cut(s) 34, 411, 501, 576, 1002, 1371, 1682
Alw21I GWGCWC 3 cut(s) 652, 928, 1937
Alw26I GTCTC 6 cut(s) 296, 393, 461, 830, 1012, 1799
AlwI GGATC 2 cut(s) 1044, 1208
AlwNI CAGNNNCTG 3 cut(s) 116, 583, 788
Ama87I CYCGRG 2 cut(s) 217, 1207
AoxI GGCC 3 cut(s) 221, 1599, 1863
ApoI RAATTY 4 cut(s) 173, 360, 1186, 1452
Asp700I GAANNNNTTC 1 cut(s) 900
AspLEI GCGC 2 cut(s) 261, 263
AspS9I GGNCC 3 cut(s) 554, 1307, 1615
AsuC2I CCSGG 3 cut(s) 592, 1208, 1209
AsuHPI GGTGA 6 cut(s) 83, 190, 429, 536, 1223, 1607
AvaI CYCGRG 2 cut(s) 217, 1207
AvaII GGWCC 3 cut(s) 554, 1307, 1615
BaeGI GKGCMC 1 cut(s) 83
BanII GRGCYC 2 cut(s) 928, 1937
BarI GAAGNNNNNNTAC 2 cut(s) 1345, 1377
BbsI GAAGAC 2 cut(s) 1252, 1513
Bbv12I GWGCWC 3 cut(s) 652, 928, 1937
BbvCI CCTCAGC 1 cut(s) 246
BccI CCATC 5 cut(s) 179, 638, 913, 1225, 1457
BceAI ACGGC 1 cut(s) 1850
BcgI CGANNNNNNTGC 6 cut(s) 437, 467, 471, 501, 1552, 1586
BciT130I CCWGG 7 cut(s) 36, 413, 503, 578, 1004, 1373, 1684
BclI TGATCA 3 cut(s) 565, 631, 931
BcnI CCSGG 3 cut(s) 592, 1208, 1209
BcoDI GTCTC 6 cut(s) 296, 393, 461, 830, 1012, 1799
BfaI CTAG 1 cut(s) 1239
BfmI CTRYAG 2 cut(s) 108, 1773
Bme18I GGWCC 3 cut(s) 554, 1307, 1615
BmeRI GACNNNNNGTC 1 cut(s) 782
BmeT110I CYCGRG 2 cut(s) 217, 1207
BmgT120I GGNCC 3 cut(s) 554, 1307, 1615
BmiI GGNNCC 1 cut(s) 1617
BmrI ACTGGG 2 cut(s) 1561, 1614
BmsI GCATC 2 cut(s) 256, 1078
BmuI ACTGGG 2 cut(s) 1561, 1614
BpiI GAAGAC 2 cut(s) 1252, 1513
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 2 cut(s) 246, 1498
BpuEI CTTGAG 2 cut(s) 854, 1008
BpuMI CCSGG 3 cut(s) 592, 1208, 1209
Bsa29I ATCGAT 1 cut(s) 963
BsaAI YACGTR 1 cut(s) 843
BsaBI GATNNNNATC 1 cut(s) 1011
BsaI GGTCTC 2 cut(s) 1012, 1799
BsaJI CCNNGG 6 cut(s) 576, 590, 1064, 1108, 1207, 1488
BsaWI WCCGGW 1 cut(s) 1714
BsaXI ACNNNNNCTCC 2 cut(s) 1234, 1264
Bsc4I CCNNNNNNNGG 2 cut(s) 1463, 1737
Bse118I RCCGGY 1 cut(s) 1168
Bse1I ACTGG 6 cut(s) 88, 332, 793, 1291, 1567, 1609
Bse3DI GCAATG 3 cut(s) 701, 1277, 1331
Bse8I GATNNNNATC 1 cut(s) 1011
BseBI CCWGG 7 cut(s) 36, 413, 503, 578, 1004, 1373, 1684
BseCI ATCGAT 1 cut(s) 963
BseDI CCNNGG 6 cut(s) 576, 590, 1064, 1108, 1207, 1488
BseGI GGATG 4 cut(s) 386, 1484, 1692, 1783
BseJI GATNNNNATC 1 cut(s) 1011
BseLI CCNNNNNNNGG 2 cut(s) 1463, 1737
BseMI GCAATG 3 cut(s) 701, 1277, 1331
BseMII CTCAG 5 cut(s) 237, 291, 654, 868, 1007
BseNI ACTGG 6 cut(s) 88, 332, 793, 1291, 1567, 1609
BsePI GCGCGC 1 cut(s) 259
BseSI GKGCMC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 1035
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 3 cut(s) 223, 1601, 1865
BshVI ATCGAT 1 cut(s) 963
BsiHKAI GWGCWC 3 cut(s) 652, 928, 1937
BsiHKCI CYCGRG 2 cut(s) 217, 1207
BsiSI CCGG 4 cut(s) 592, 1169, 1208, 1715
BslFI GGGAC 3 cut(s) 122, 248, 1601
BslI CCNNNNNNNGG 2 cut(s) 1463, 1737
BsmAI GTCTC 6 cut(s) 296, 393, 461, 830, 1012, 1799
BsmBI CGTCTC 1 cut(s) 393
BsmFI GGGAC 3 cut(s) 122, 248, 1601
BsmI GAATGC 1 cut(s) 1797
BsnI GGCC 3 cut(s) 223, 1601, 1865
Bso31I GGTCTC 2 cut(s) 1012, 1799
BsoBI CYCGRG 2 cut(s) 217, 1207
Bsp1286I GDGCHC 4 cut(s) 83, 652, 928, 1937
Bsp143I GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
Bsp19I CCATGG 1 cut(s) 1064
BspACI CCGC 5 cut(s) 23, 1094, 1759, 1825, 1852
BspANI GGCC 3 cut(s) 223, 1601, 1865
BspCNI CTCAG 5 cut(s) 238, 292, 655, 867, 1008
BspDI ATCGAT 1 cut(s) 963
BspFNI CGCG 1 cut(s) 261
BspHI TCATGA 2 cut(s) 634, 1443
BspLI GGNNCC 1 cut(s) 1617
BspMAI CTGCAG 2 cut(s) 112, 1777
BspPI GGATC 2 cut(s) 1044, 1208
BspTNI GGTCTC 2 cut(s) 1012, 1799
BsrBI CCGCTC 1 cut(s) 1854
BsrDI GCAATG 3 cut(s) 701, 1277, 1331
BsrFI RCCGGY 1 cut(s) 1168
BsrI ACTGG 6 cut(s) 88, 332, 793, 1291, 1567, 1609
BssAI RCCGGY 1 cut(s) 1168
BssECI CCNNGG 6 cut(s) 576, 590, 1064, 1108, 1207, 1488
BssHII GCGCGC 1 cut(s) 259
BssMI GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
BssNAI GTATAC 1 cut(s) 191
BssT1I CCWWGG 3 cut(s) 1064, 1108, 1488
Bst1107I GTATAC 1 cut(s) 191
Bst2UI CCWGG 7 cut(s) 36, 413, 503, 578, 1004, 1373, 1684
Bst4CI ACNGT 5 cut(s) 538, 784, 850, 1535, 1913
Bst6I CTCTTC 1 cut(s) 1550
BstBAI YACGTR 1 cut(s) 843
BstC8I GCNNGC 4 cut(s) 261, 924, 1816, 1902
BstDEI CTNAG 8 cut(s) 246, 300, 653, 663, 854, 1016, 1428, 1498
BstDSI CCRYGG 1 cut(s) 1064
BstF5I GGATG 4 cut(s) 386, 1484, 1692, 1783
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 2 cut(s) 261, 263
BstKTI GATC 9 cut(s) 295, 568, 634, 934, 1032, 1039, 1216, 1258, 1648
BstMAI GTCTC 6 cut(s) 296, 393, 461, 830, 1012, 1799
BstMBI GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 337, 1824
BstNI CCWGG 7 cut(s) 36, 413, 503, 578, 1004, 1373, 1684
BstNSI RCATGY 2 cut(s) 809, 1509
BstSFI CTRYAG 2 cut(s) 108, 1773
BstSLI GKGCMC 1 cut(s) 83
BstUI CGCG 1 cut(s) 261
BstV2I GAAGAC 2 cut(s) 1252, 1513
BstX2I RGATCY 1 cut(s) 1036
BstYI RGATCY 1 cut(s) 1036
BstZ17I GTATAC 1 cut(s) 191
Bsu15I ATCGAT 1 cut(s) 963
BsuRI GGCC 3 cut(s) 223, 1601, 1865
BsuTUI ATCGAT 1 cut(s) 963
BtgI CCRYGG 1 cut(s) 1064
BtsCI GGATG 4 cut(s) 386, 1484, 1692, 1783
BtsIMutI CAGTG 7 cut(s) 81, 114, 581, 786, 1148, 1298, 1602
Cac8I GCNNGC 4 cut(s) 261, 924, 1816, 1902
CaiI CAGNNNCTG 3 cut(s) 116, 583, 788
CciI TCATGA 2 cut(s) 634, 1443
CfoI GCGC 2 cut(s) 261, 263
Cfr10I RCCGGY 1 cut(s) 1168
Cfr13I GGNCC 3 cut(s) 554, 1307, 1615
Cfr9I CCCGGG 1 cut(s) 1207
ClaI ATCGAT 1 cut(s) 963
CseI GACGC 1 cut(s) 1136
CsiI ACCWGGT 1 cut(s) 411
Csp6I GTAC 1 cut(s) 851
CviAII CATG 8 cut(s) 635, 695, 806, 1065, 1444, 1506, 1551, 1627
CviQI GTAC 1 cut(s) 851
DdeI CTNAG 8 cut(s) 246, 300, 653, 663, 854, 1016, 1428, 1498
DpnI GATC 9 cut(s) 294, 567, 633, 933, 1031, 1038, 1215, 1257, 1647
DpnII GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
DrdI GACNNNNNNGTC 1 cut(s) 1517
DriI GACNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 1517
EaeI YGGCCR 1 cut(s) 1863
Eam1104I CTCTTC 1 cut(s) 1550
Eam1105I GACNNNNNGTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 1550
Ecl136II GAGCTC 2 cut(s) 926, 1935
Eco130I CCWWGG 3 cut(s) 1064, 1108, 1488
Eco24I GRGCYC 2 cut(s) 928, 1937
Eco31I GGTCTC 2 cut(s) 1012, 1799
Eco47I GGWCC 3 cut(s) 554, 1307, 1615
Eco53kI GAGCTC 2 cut(s) 926, 1935
Eco57I CTGAAG 3 cut(s) 379, 512, 912
Eco88I CYCGRG 2 cut(s) 217, 1207
EcoICRI GAGCTC 2 cut(s) 926, 1935
EcoO109I RGGNCCY 2 cut(s) 554, 1615
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 7 cut(s) 34, 411, 501, 576, 1002, 1371, 1682
EcoT14I CCWWGG 3 cut(s) 1064, 1108, 1488
EcoT38I GRGCYC 2 cut(s) 928, 1937
ErhI CCWWGG 3 cut(s) 1064, 1108, 1488
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 8 cut(s) 638, 698, 809, 1068, 1447, 1509, 1554, 1630
FaqI GGGAC 3 cut(s) 122, 248, 1601
FatI CATG 8 cut(s) 634, 694, 805, 1064, 1443, 1505, 1550, 1626
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 3 cut(s) 565, 631, 931
FblI GTMKAC 2 cut(s) 190, 207
FokI GGATG 4 cut(s) 393, 1471, 1699, 1770
FriOI GRGCYC 2 cut(s) 928, 1937
FspBI CTAG 1 cut(s) 1239
GlaI GCGC 2 cut(s) 260, 262
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 3 cut(s) 223, 1601, 1865
HapII CCGG 4 cut(s) 592, 1169, 1208, 1715
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 2 cut(s) 261, 263
Hin1II CATG 8 cut(s) 638, 698, 809, 1068, 1447, 1509, 1554, 1630
Hin6I GCGC 2 cut(s) 259, 261
HinP1I GCGC 2 cut(s) 259, 261
HincII GTYRAC 2 cut(s) 208, 948
HindII GTYRAC 2 cut(s) 208, 948
HindIII AAGCTT 2 cut(s) 94, 386
HpaII CCGG 4 cut(s) 592, 1169, 1208, 1715
HphI GGTGA 6 cut(s) 83, 190, 429, 536, 1223, 1607
Hpy166II GTNNAC 5 cut(s) 191, 208, 846, 948, 1277
Hpy188I TCNGA 6 cut(s) 398, 715, 936, 1017, 1176, 1540
Hpy8I GTNNAC 5 cut(s) 191, 208, 846, 948, 1277
HpyAV CCTTC 6 cut(s) 551, 716, 720, 752, 1063, 1347
HpyCH4III ACNGT 5 cut(s) 538, 784, 850, 1535, 1913
HpyCH4IV ACGT 3 cut(s) 313, 842, 1249
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 337, 1824
HpyF3I CTNAG 8 cut(s) 246, 300, 653, 663, 854, 1016, 1428, 1498
HpySE526I ACGT 3 cut(s) 313, 842, 1249
Hsp92II CATG 8 cut(s) 638, 698, 809, 1068, 1447, 1509, 1554, 1630
HspAI GCGC 2 cut(s) 259, 261
Ksp22I TGATCA 3 cut(s) 565, 631, 931
Kzo9I GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
LmnI GCTCC 3 cut(s) 931, 1081, 1733
LweI GCATC 2 cut(s) 256, 1078
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 1 cut(s) 1239
MaeII ACGT 3 cut(s) 313, 842, 1249
MaeIII GTNAC 6 cut(s) 196, 435, 784, 1146, 1234, 1508
MalI GATC 9 cut(s) 294, 567, 633, 933, 1031, 1038, 1215, 1257, 1647
MbiI CCGCTC 1 cut(s) 1854
MboI GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
MboII GAAGA 8 cut(s) 169, 239, 905, 1097, 1257, 1472, 1513, 1567
MflI RGATCY 1 cut(s) 1036
MhlI GDGCHC 4 cut(s) 83, 652, 928, 1937
MlyI GAGTC 2 cut(s) 298, 1553
MroXI GAANNNNTTC 1 cut(s) 900
MseI TTAA 3 cut(s) 668, 729, 1769
MslI CAYNNNNRTG 1 cut(s) 1685
MspI CCGG 4 cut(s) 592, 1169, 1208, 1715
Mva1269I GAATGC 1 cut(s) 1797
MvaI CCWGG 7 cut(s) 36, 413, 503, 578, 1004, 1373, 1684
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 337, 1824
NciI CCSGG 3 cut(s) 592, 1208, 1209
NcoI CCATGG 1 cut(s) 1064
NdeI CATATG 1 cut(s) 283
NdeII GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
NlaIII CATG 8 cut(s) 638, 698, 809, 1068, 1447, 1509, 1554, 1630
NlaIV GGNNCC 1 cut(s) 1617
NmuCI GTSAC 5 cut(s) 196, 435, 784, 1146, 1508
NspI RCATGY 2 cut(s) 809, 1509
PaeR7I CTCGAG 1 cut(s) 217
PagI TCATGA 2 cut(s) 634, 1443
PauI GCGCGC 1 cut(s) 259
PctI GAATGC 1 cut(s) 1797
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 9 cut(s) 57, 119, 572, 1203, 1376, 1418, 1493, 1652, 1676
PflMI CCANNNNNTGG 1 cut(s) 1463
PleI GAGTC 2 cut(s) 298, 1552
PpsI GAGTC 2 cut(s) 298, 1552
Ppu21I YACGTR 1 cut(s) 843
PpuMI RGGWCCY 2 cut(s) 554, 1615
Psp124BI GAGCTC 2 cut(s) 928, 1937
Psp1406I AACGTT 1 cut(s) 313
Psp5II RGGWCCY 2 cut(s) 554, 1615
Psp6I CCWGG 7 cut(s) 34, 411, 501, 576, 1002, 1371, 1682
PspGI CCWGG 7 cut(s) 34, 411, 501, 576, 1002, 1371, 1682
PspN4I GGNNCC 1 cut(s) 1617
PspPI GGNCC 3 cut(s) 554, 1307, 1615
PspPPI RGGWCCY 2 cut(s) 554, 1615
PspXI VCTCGAGB 1 cut(s) 217
PsrI GAACNNNNNNTAC 2 cut(s) 466, 498
PstI CTGCAG 2 cut(s) 112, 1777
PstNI CAGNNNCTG 3 cut(s) 116, 583, 788
PsuI RGATCY 1 cut(s) 1036
PteI GCGCGC 1 cut(s) 259
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
RseI CAYNNNNRTG 1 cut(s) 1685
SacI GAGCTC 2 cut(s) 928, 1937
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 3 cut(s) 668, 729, 1769
Sau3AI GATC 9 cut(s) 292, 565, 631, 931, 1029, 1036, 1213, 1255, 1645
Sau96I GGNCC 3 cut(s) 554, 1307, 1615
SchI GAGTC 2 cut(s) 298, 1553
SduI GDGCHC 4 cut(s) 83, 652, 928, 1937
SexAI ACCWGGT 1 cut(s) 411
SfaNI GCATC 2 cut(s) 256, 1078
SfcI CTRYAG 2 cut(s) 108, 1773
Sfr274I CTCGAG 1 cut(s) 217
SinI GGWCC 3 cut(s) 554, 1307, 1615
SlaI CTCGAG 1 cut(s) 217
SmaI CCCGGG 1 cut(s) 1209
SmiMI CAYNNNNRTG 1 cut(s) 1685
SmlI CTYRAG 3 cut(s) 217, 833, 1023
SmoI CTYRAG 3 cut(s) 217, 833, 1023
SsiI CCGC 5 cut(s) 23, 1094, 1759, 1825, 1852
SspI AATATT 1 cut(s) 915
SspMI CTAG 1 cut(s) 1239
SstI GAGCTC 2 cut(s) 928, 1937
StyI CCWWGG 3 cut(s) 1064, 1108, 1488
TaaI ACNGT 5 cut(s) 538, 784, 850, 1535, 1913
TaiI ACGT 3 cut(s) 316, 845, 1252
TaqII GACCGA 1 cut(s) 1823
TatI WGTACW 1 cut(s) 850
TauI GCSGC 1 cut(s) 1827
TfiI GAWTC 9 cut(s) 57, 119, 572, 1203, 1376, 1418, 1493, 1652, 1676
Tru1I TTAA 3 cut(s) 668, 729, 1769
Tru9I TTAA 3 cut(s) 668, 729, 1769
TscAI CASTG 7 cut(s) 88, 121, 588, 793, 1155, 1298, 1609
TseFI GTSAC 5 cut(s) 196, 435, 784, 1146, 1508
Tsp45I GTSAC 5 cut(s) 196, 435, 784, 1146, 1508
TspDTI ATGAA 5 cut(s) 783, 1098, 1431, 1473, 1644
TspMI CCCGGG 1 cut(s) 1207
TspRI CASTG 7 cut(s) 88, 121, 588, 793, 1155, 1298, 1609
Van91I CCANNNNNTGG 1 cut(s) 1463
VpaK11BI GGWCC 3 cut(s) 554, 1307, 1615
XapI RAATTY 4 cut(s) 173, 360, 1186, 1452
XceI RCATGY 2 cut(s) 809, 1509
XhoI CTCGAG 1 cut(s) 217
XmaI CCCGGG 1 cut(s) 1207
XmiI GTMKAC 2 cut(s) 190, 207
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 1 cut(s) 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.