Rh3BG062900

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0009905
Physical Location & Seq
Forward (+)
4214921 .. 4222968
8048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG062900.1

Sequence Viewer

Length: 1950 bp
ATGGCGAAACCTGCAGCAGGAATCGCGAACCAGAACCACGGCGGCCGGACCGCTGTTCGGATTGTTGTGGCGGGAGACCGAAGTACCGGAAAATCGAGCTTGATTGTAACTGCGGCGGCGGAGAACTTTCCGGCTAATGTGCCTCCGCTGCTGCCGCCTACAAGGCTGCCTGAAGATTTCTACCCCGAGCGCGTGCCTATCACGATTATTGATACTTCTTCTAGACCAGAGGATAGTGGTAAAGTAGCTGAAGATCTGAAGGCTGCTGATGCAGTTGTGCTAACTTATGCGTGTGATCAGCCTCAGACGCTTGACCGGTTGAGTAACTTTTGGCTTCCAAAGCTTCGTCAGCTAGAGGTAAAGGTCCCTGTTATAGTGGTTGGTTGTAAGCTCGATTTGAGAGACGAGAATCAACAGGTGAGCCTGGAGCAAGTGATGTCGCCAATAATGCAACAGTTTCGGGAAATTGAAACCTGCATCGAGTGTTCAGCATATAAACATATTCAGATTCCTGAGGTTTTTTACTATGCACAAAAAGCTGTTCTTCACCCAACAGCCCCATTATTCGATCAGGAAACACAGACTTTGAAGGACCAATGCGTGCGAGCCTTGAAGCGGATATTCATTCTCTGTGATCATGATAGGGATGGTGCCCTTAGTGATGCAGAATTGAACGATTTCCAGGTTAAATGTTTCAATGCTCCACTACAACCTTCTGAAATAGTGGGCGTGAAGAGGGTTGTGCAAGAAAAGTTACCGGAAGGAGTCAATGACCGGGGGCTTACACTGACAGGATTCCTCTTCCTGCATGCACTATTTATAGAGAAGGGACGTCTAGAGACAACTTGGACTGTACTCAGGAAGTTTGGCTATAACAACGATATACGACTTTCAGAAGAACTAATTCCATCACTCCCAAAACGGACTCCTGATCAGAGCATGGAGCTGACAAATGAAGCAATCGATTTTCTGAGTGCGACCTTCAACTTGTTTGACAATGATGGTGATGGGGCCCTAAGACCACGTGAACTGGAAGAACTCTTTTCGACTGCTCCTGCAAGTCCATTTAGTGAATTTCCATACAAGGATGCTGAAGAGAGAAATGCATTTGGAGGCCTATCACTTGATGGGTTCTTGTCACTGTGGGCCCTCATGACACTCCTAAACCCAGCCTCTACCCTGGAGAATCTTATATACATTGGTTATGCCGGTGATGTTTCGTCTGCAATCCGTGTGACTAGGAAAAGGCGTTTTGATCGCAAGAAGCAACAATCAGAACGAAATGTTTTCCAATGCTTCGTCTTCGGCCCAAAGAAGGCTGGAAAGTCTGCATTGTTGGATTCTTTTCTTGGAAGGCCATTTTCTGATAACTATTATCCAACTACCGAGGAGCATTATGCAGTTAATGTTGTTGATCAGCCTGGGGGAACCAAGAAAATCCTAGTGTTGAGAGAGATTGCTGAAGATGGAGTTAGCAAATTACTATCTAGTAAAGAGGCTTTGGCTGCTTGTGACATAGCTGTGTTTGTTCATGACAGTTCTGATGAATTGTCCTGGAAGAAAGCAACAGACTTGCTGGTAGAAGTTGCTAGTCATGGTGAGAATACTGGTTTTGAGGTGCCTTGCCTCATTGTTGCAGCTAAAGATGATCTGGATTCATTTCCATTGGCCATACAGCATTCTACAAGGGTTAGTCAGGATATGGGAACAGAGGCTCCTATTCCTATTAGCACAAAGTTGGGTGATTTTAACAATGTATTTCGTAAAATTGTAAGTGCTGCGGAGCACCCTCATTTGAGTATTCCCGAGACGGAGGCTGGAAGGAGTCGCAAGCAATATCACAGGCTCATAAACCGCTCGCTTATGTTTGTTTCAGTGGGAGCTGCAGTGGCCATTGTTGGACTGGCAGCCTACCGTGTCTATGCTGCAAGGAAGAATGCCTCCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006810 GO:0006839 GO:0006915 GO:0006928 GO:0006996 GO:0007005 GO:0007006 GO:0007017 GO:0007018 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008219 GO:0008637 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010154 GO:0010970 GO:0012501 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0019725 GO:0019867 GO:0022414 GO:0023052 GO:0030154 GO:0030705 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032989 GO:0034643 GO:0035794 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044706 GO:0046902 GO:0046907 GO:0047497 GO:0048316 GO:0048468 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0051704 GO:0051716 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0072384 GO:0090559 GO:0097190 GO:0097345 GO:0098573 GO:0098588 GO:0098805 GO:0099111 GO:1902108 GO:1902110 GO:1902686 GO:1905710
KEGG Pathways
Metabolic & Signaling
ko04137 ko04214 map04137 map04214
Pfam Domains
Protein Families
EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

649

Amino Acids

71.95

Weight (kDa)

5.63

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 20 - 181 1.2e-12 Ras family
EF_assoc_2 PF08356 235 - 319 2.7e-29 EF hand associated
EF_assoc_1 PF08355 360 - 424 3.6e-21 EF hand associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 835
Acc36I ACCTGC 2 cut(s) 19, 482
AccB1I GGYRCC 2 cut(s) 650, 1618
AccBSI CCGCTC 1 cut(s) 1857
AccII CGCG 2 cut(s) 26, 192
AcoI YGGCCR 3 cut(s) 43, 1668, 1890
AcsI RAATTY 1 cut(s) 1073
AcuI CTGAAG 5 cut(s) 192, 270, 278, 1113, 1482
AcvI CACGTG 1 cut(s) 1025
AcyI GRCGYC 1 cut(s) 832
AfaI GTAC 2 cut(s) 85, 855
AfiI CCNNNNNNNGG 4 cut(s) 17, 57, 615, 1315
AgeI ACCGGT 1 cut(s) 315
AgsI TTSAA 6 cut(s) 470, 589, 613, 673, 697, 985
AjnI CCWGG 5 cut(s) 423, 681, 1179, 1420, 1553
AloI GAACNNNNNNTCC 2 cut(s) 1699, 1731
Alw21I GWGCWC 1 cut(s) 1788
Alw26I GTCTC 4 cut(s) 69, 396, 833, 1802
Ama87I CYCGRG 2 cut(s) 185, 1805
AoxI GGCC 8 cut(s) 43, 1011, 1114, 1146, 1306, 1355, 1668, 1890
ApaI GGGCCC 2 cut(s) 1015, 1150
ApoI RAATTY 1 cut(s) 1073
AsiGI ACCGGT 1 cut(s) 315
Asp700I GAANNNNTTC 2 cut(s) 677, 903
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 8 cut(s) 48, 364, 592, 1011, 1012, 1146, 1147, 1307
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 6 cut(s) 430, 539, 1016, 1223, 1610, 1754
AvaI CYCGRG 2 cut(s) 185, 1805
AvaII GGWCC 3 cut(s) 48, 364, 592
AxyI CCTNAGG 1 cut(s) 513
BaeGI GKGCMC 3 cut(s) 655, 1015, 1150
BaeI ACNNNNGTAYC 2 cut(s) 67, 100
BalI TGGCCA 2 cut(s) 1670, 1892
BanI GGYRCC 2 cut(s) 650, 1618
BanII GRGCYC 2 cut(s) 1015, 1150
BbrPI CACGTG 1 cut(s) 1025
BbsI GAAGAC 1 cut(s) 1294
Bbv12I GWGCWC 1 cut(s) 1788
BccI CCATC 6 cut(s) 641, 916, 995, 1001, 1121, 1460
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 5 cut(s) 425, 683, 1181, 1422, 1555
BclI TGATCA 4 cut(s) 295, 634, 931, 1414
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 4 cut(s) 69, 396, 833, 1802
BfaI CTAG 7 cut(s) 222, 353, 836, 1239, 1442, 1488, 1590
BfmI CTRYAG 2 cut(s) 12, 1884
BfuAI ACCTGC 2 cut(s) 19, 482
BglI GCCNNNNNGGC 1 cut(s) 163
BglII AGATCT 1 cut(s) 253
Bme1390I CCNGG 6 cut(s) 425, 683, 776, 1181, 1422, 1555
Bme18I GGWCC 3 cut(s) 48, 364, 592
BmeT110I CYCGRG 2 cut(s) 185, 1805
BmgT120I GGNCC 8 cut(s) 48, 364, 592, 1011, 1012, 1146, 1147, 1307
BmiI GGNNCC 8 cut(s) 366, 652, 1012, 1013, 1148, 1429, 1620, 1716
BmrFI CCNGG 6 cut(s) 425, 683, 776, 1181, 1422, 1555
BmsI GCATC 4 cut(s) 259, 486, 652, 1078
BpiI GAAGAC 1 cut(s) 1294
BpmI CTGGAG 3 cut(s) 446, 1202, 1927
BpuMI CCSGG 1 cut(s) 776
Bsa29I ATCGAT 1 cut(s) 963
BsaAI YACGTR 1 cut(s) 1025
BsaHI GRCGYC 1 cut(s) 832
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 5 cut(s) 37, 775, 1179, 1386, 1421
BsaWI WCCGGW 3 cut(s) 86, 315, 757
BsaXI ACNNNNNCTCC 4 cut(s) 1699, 1729, 1872, 1902
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 57, 615, 1315
Bse118I RCCGGY 2 cut(s) 315, 1208
Bse1I ACTGG 4 cut(s) 1035, 1612, 1908, 1944
Bse21I CCTNAGG 1 cut(s) 513
BseBI CCWGG 5 cut(s) 425, 683, 1181, 1422, 1555
BseCI ATCGAT 1 cut(s) 963
BseDI CCNNGG 5 cut(s) 37, 775, 1179, 1386, 1421
BseGI GGATG 2 cut(s) 652, 1093
BseLI CCNNNNNNNGG 4 cut(s) 17, 57, 615, 1315
BseMII CTCAG 4 cut(s) 317, 504, 871, 962
BseNI ACTGG 4 cut(s) 1035, 1612, 1908, 1944
BseRI GAGGAG 1 cut(s) 1403
BseSI GKGCMC 3 cut(s) 655, 1015, 1150
BseX3I CGGCCG 1 cut(s) 43
BseYI CCCAGC 1 cut(s) 1168
Bsh1236I CGCG 2 cut(s) 26, 192
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 8 cut(s) 45, 1013, 1116, 1148, 1308, 1357, 1670, 1892
BshNI GGYRCC 2 cut(s) 650, 1618
BshTI ACCGGT 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 963
BsiEI CGRYCG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 1788
BsiHKCI CYCGRG 2 cut(s) 185, 1805
BsiSI CCGG 7 cut(s) 46, 87, 131, 316, 758, 775, 1209
BslFI GGGAC 2 cut(s) 350, 843
BslI CCNNNNNNNGG 4 cut(s) 17, 57, 615, 1315
BsmAI GTCTC 4 cut(s) 69, 396, 833, 1802
BsmBI CGTCTC 2 cut(s) 396, 1802
BsmFI GGGAC 2 cut(s) 350, 843
BsmI GAATGC 2 cut(s) 1678, 1942
BsnI GGCC 8 cut(s) 45, 1013, 1116, 1148, 1308, 1357, 1670, 1892
Bso31I GGTCTC 1 cut(s) 69
BsoBI CYCGRG 2 cut(s) 185, 1805
Bsp120I GGGCCC 2 cut(s) 1011, 1146
Bsp1286I GDGCHC 4 cut(s) 655, 1015, 1150, 1788
Bsp143I GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
Bsp68I TCGCGA 1 cut(s) 26
BspANI GGCC 8 cut(s) 45, 1013, 1116, 1148, 1308, 1357, 1670, 1892
BspCNI CTCAG 4 cut(s) 316, 505, 870, 963
BspDI ATCGAT 1 cut(s) 963
BspFNI CGCG 2 cut(s) 26, 192
BspHI TCATGA 3 cut(s) 637, 1152, 1531
BspLI GGNNCC 8 cut(s) 366, 652, 1012, 1013, 1148, 1429, 1620, 1716
BspMAI CTGCAG 2 cut(s) 16, 1888
BspMI ACCTGC 2 cut(s) 19, 482
BspT107I GGYRCC 2 cut(s) 650, 1618
BspTNI GGTCTC 1 cut(s) 69
BsrBI CCGCTC 1 cut(s) 1857
BsrFI RCCGGY 2 cut(s) 315, 1208
BsrI ACTGG 4 cut(s) 1035, 1612, 1908, 1944
BssAI RCCGGY 2 cut(s) 315, 1208
BssECI CCNNGG 5 cut(s) 37, 775, 1179, 1386, 1421
BssMI GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
BssNI GRCGYC 1 cut(s) 832
Bst2UI CCWGG 5 cut(s) 425, 683, 1181, 1422, 1555
Bst4CI ACNGT 5 cut(s) 456, 853, 1143, 1538, 1916
Bst6I CTCTTC 3 cut(s) 728, 806, 1089
BstACI GRCGYC 1 cut(s) 832
BstBAI YACGTR 1 cut(s) 1025
BstC8I GCNNGC 6 cut(s) 194, 602, 606, 810, 1832, 1859
BstDEI CTNAG 6 cut(s) 303, 513, 656, 857, 971, 1016
BstDSI CCRYGG 1 cut(s) 37
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 652, 1093
BstFNI CGCG 2 cut(s) 26, 192
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 8 cut(s) 256, 298, 571, 637, 934, 1258, 1417, 1651
BstMAI GTCTC 4 cut(s) 69, 396, 833, 1802
BstMBI GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
BstMCI CGRYCG 1 cut(s) 46
BstNI CCWGG 5 cut(s) 425, 683, 1181, 1422, 1555
BstNSI RCATGY 1 cut(s) 812
BstSCI CCNGG 6 cut(s) 423, 681, 774, 1179, 1420, 1553
BstSFI CTRYAG 2 cut(s) 12, 1884
BstSLI GKGCMC 3 cut(s) 655, 1015, 1150
BstUI CGCG 2 cut(s) 26, 192
BstV2I GAAGAC 1 cut(s) 1294
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BstZI CGGCCG 1 cut(s) 43
Bsu15I ATCGAT 1 cut(s) 963
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 8 cut(s) 45, 1013, 1116, 1148, 1308, 1357, 1670, 1892
BsuTUI ATCGAT 1 cut(s) 963
BtgI CCRYGG 1 cut(s) 37
BtsCI GGATG 2 cut(s) 652, 1093
BtsI GCAGTG 1 cut(s) 1893
BtsIMutI CAGTG 4 cut(s) 785, 1139, 1881, 1893
BtuMI TCGCGA 1 cut(s) 26
BveI ACCTGC 2 cut(s) 19, 482
Cac8I GCNNGC 6 cut(s) 194, 602, 606, 810, 1832, 1859
CciI TCATGA 3 cut(s) 637, 1152, 1531
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 2 cut(s) 315, 1208
Cfr13I GGNCC 8 cut(s) 48, 364, 592, 1011, 1012, 1146, 1147, 1307
ClaI ATCGAT 1 cut(s) 963
CpoI CGGWCCG 1 cut(s) 48
CseI GACGC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 84, 854
CspAI ACCGGT 1 cut(s) 315
CspI CGGWCCG 1 cut(s) 48
CviAII CATG 6 cut(s) 638, 809, 940, 1153, 1532, 1595
CviQI GTAC 2 cut(s) 84, 854
DdeI CTNAG 6 cut(s) 303, 513, 656, 857, 971, 1016
DpnI GATC 8 cut(s) 255, 297, 570, 636, 933, 1257, 1416, 1650
DpnII GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
EaeI YGGCCR 3 cut(s) 43, 1668, 1890
EagI CGGCCG 1 cut(s) 43
Eam1104I CTCTTC 3 cut(s) 728, 806, 1089
EarI CTCTTC 3 cut(s) 728, 806, 1089
EciI GGCGGA 1 cut(s) 134
EclXI CGGCCG 1 cut(s) 43
Eco147I AGGCCT 1 cut(s) 1116
Eco24I GRGCYC 2 cut(s) 1015, 1150
Eco31I GGTCTC 1 cut(s) 69
Eco47I GGWCC 3 cut(s) 48, 364, 592
Eco52I CGGCCG 1 cut(s) 43
Eco57I CTGAAG 5 cut(s) 192, 270, 278, 1113, 1482
Eco72I CACGTG 1 cut(s) 1025
Eco81I CCTNAGG 1 cut(s) 513
Eco88I CYCGRG 2 cut(s) 185, 1805
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 4 cut(s) 364, 1011, 1012, 1147
EcoRII CCWGG 5 cut(s) 423, 681, 1179, 1420, 1553
EcoT22I ATGCAT 1 cut(s) 1108
EcoT38I GRGCYC 2 cut(s) 1015, 1150
Esp3I CGTCTC 2 cut(s) 396, 1802
FaeI CATG 6 cut(s) 641, 812, 943, 1156, 1535, 1598
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 2 cut(s) 350, 843
FatI CATG 6 cut(s) 637, 808, 939, 1152, 1531, 1594
FauI CCCGC 1 cut(s) 64
FbaI TGATCA 4 cut(s) 295, 634, 931, 1414
FokI GGATG 2 cut(s) 659, 1100
FriOI GRGCYC 2 cut(s) 1015, 1150
FspBI CTAG 7 cut(s) 222, 353, 836, 1239, 1442, 1488, 1590
GlaI GCGC 1 cut(s) 191
GsaI CCCAGC 1 cut(s) 1172
GsuI CTGGAG 3 cut(s) 446, 1202, 1927
HaeIII GGCC 8 cut(s) 45, 1013, 1116, 1148, 1308, 1357, 1670, 1892
HapII CCGG 7 cut(s) 46, 87, 131, 316, 758, 775, 1209
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 832
Hin1II CATG 6 cut(s) 641, 812, 943, 1156, 1535, 1598
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 341
HpaII CCGG 7 cut(s) 46, 87, 131, 316, 758, 775, 1209
HphI GGTGA 6 cut(s) 430, 539, 1016, 1223, 1610, 1754
Hpy166II GTNNAC 1 cut(s) 1028
Hpy8I GTNNAC 1 cut(s) 1028
HpyAV CCTTC 9 cut(s) 253, 583, 723, 755, 820, 991, 1309, 1347, 1815
HpyCH4III ACNGT 5 cut(s) 456, 853, 1143, 1538, 1916
HpyCH4IV ACGT 2 cut(s) 832, 1024
HpyF3I CTNAG 6 cut(s) 303, 513, 656, 857, 971, 1016
HpySE526I ACGT 2 cut(s) 832, 1024
Hsp92I GRCGYC 1 cut(s) 832
Hsp92II CATG 6 cut(s) 641, 812, 943, 1156, 1535, 1598
HspAI GCGC 1 cut(s) 190
Ksp22I TGATCA 4 cut(s) 295, 634, 931, 1414
Kzo9I GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
LmnI GCTCC 8 cut(s) 427, 706, 943, 1057, 1390, 1720, 1783, 1880
LweI GCATC 4 cut(s) 259, 486, 652, 1078
MaeI CTAG 7 cut(s) 222, 353, 836, 1239, 1442, 1488, 1590
MaeII ACGT 2 cut(s) 832, 1024
MaeIII GTNAC 6 cut(s) 106, 323, 753, 1137, 1234, 1511
MalI GATC 8 cut(s) 255, 297, 570, 636, 933, 1257, 1416, 1650
MbiI CCGCTC 1 cut(s) 1857
MboI GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 4 cut(s) 655, 1015, 1150, 1788
MlsI TGGCCA 2 cut(s) 1670, 1892
MluCI AATT 7 cut(s) 465, 668, 903, 1073, 1478, 1547, 1767
MluNI TGGCCA 2 cut(s) 1670, 1892
MlyI GAGTC 3 cut(s) 774, 919, 1834
MmeI TCCRAC 3 cut(s) 1317, 1403, 1879
Mox20I TGGCCA 2 cut(s) 1670, 1892
Mph1103I ATGCAT 1 cut(s) 1108
MroXI GAANNNNTTC 2 cut(s) 677, 903
MscI TGGCCA 2 cut(s) 1670, 1892
MseI TTAA 4 cut(s) 687, 1404, 1749, 1948
MslI CAYNNNNRTG 1 cut(s) 1520
Msp20I TGGCCA 2 cut(s) 1670, 1892
MspA1I CMGCKG 2 cut(s) 53, 148
MspI CCGG 7 cut(s) 46, 87, 131, 316, 758, 775, 1209
MspR9I CCNGG 6 cut(s) 425, 683, 776, 1181, 1422, 1555
Mva1269I GAATGC 2 cut(s) 1678, 1942
MvaI CCWGG 5 cut(s) 425, 683, 1181, 1422, 1555
MvnI CGCG 2 cut(s) 26, 192
NciI CCSGG 1 cut(s) 776
NdeII GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
NlaIII CATG 6 cut(s) 641, 812, 943, 1156, 1535, 1598
NlaIV GGNNCC 8 cut(s) 366, 652, 1012, 1013, 1148, 1429, 1620, 1716
NmuCI GTSAC 3 cut(s) 1137, 1234, 1511
NruI TCGCGA 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 1108
NspI RCATGY 1 cut(s) 812
PaeI GCATGC 1 cut(s) 812
PagI TCATGA 3 cut(s) 637, 1152, 1531
PceI AGGCCT 1 cut(s) 1116
PctI GAATGC 2 cut(s) 1678, 1942
PdmI GAANNNNTTC 2 cut(s) 677, 903
PfeI GAWTC 7 cut(s) 21, 409, 508, 795, 1186, 1340, 1655
PfoI TCCNGGA 1 cut(s) 1553
PinAI ACCGGT 1 cut(s) 315
PleI GAGTC 3 cut(s) 773, 919, 1833
PmaCI CACGTG 1 cut(s) 1025
PmlI CACGTG 1 cut(s) 1025
PpsI GAGTC 3 cut(s) 773, 919, 1833
Ppu21I YACGTR 1 cut(s) 1025
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 5 cut(s) 423, 681, 1179, 1420, 1553
PspCI CACGTG 1 cut(s) 1025
PspFI CCCAGC 1 cut(s) 1168
PspGI CCWGG 5 cut(s) 423, 681, 1179, 1420, 1553
PspN4I GGNNCC 8 cut(s) 366, 652, 1012, 1013, 1148, 1429, 1620, 1716
PspOMI GGGCCC 2 cut(s) 1011, 1146
PspPI GGNCC 8 cut(s) 48, 364, 592, 1011, 1012, 1146, 1147, 1307
PspPPI RGGWCCY 1 cut(s) 364
PstI CTGCAG 2 cut(s) 16, 1888
PsuI RGATCY 1 cut(s) 253
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 2 cut(s) 85, 855
RsaNI GTAC 2 cut(s) 84, 854
RseI CAYNNNNRTG 1 cut(s) 1520
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SaqAI TTAA 4 cut(s) 687, 1404, 1749, 1948
Sau3AI GATC 8 cut(s) 253, 295, 568, 634, 931, 1255, 1414, 1648
Sau96I GGNCC 8 cut(s) 48, 364, 592, 1011, 1012, 1146, 1147, 1307
SchI GAGTC 3 cut(s) 774, 919, 1834
ScrFI CCNGG 6 cut(s) 425, 683, 776, 1181, 1422, 1555
SduI GDGCHC 4 cut(s) 655, 1015, 1150, 1788
SfaNI GCATC 4 cut(s) 259, 486, 652, 1078
SfcI CTRYAG 2 cut(s) 12, 1884
SinI GGWCC 3 cut(s) 48, 364, 592
SmiMI CAYNNNNRTG 1 cut(s) 1520
SphI GCATGC 1 cut(s) 812
Sse9I AATT 7 cut(s) 465, 668, 903, 1073, 1478, 1547, 1767
SseBI AGGCCT 1 cut(s) 1116
SspMI CTAG 7 cut(s) 222, 353, 836, 1239, 1442, 1488, 1590
StuI AGGCCT 1 cut(s) 1116
StyD4I CCNGG 6 cut(s) 423, 681, 774, 1179, 1420, 1553
TaaI ACNGT 5 cut(s) 456, 853, 1143, 1538, 1916
TaiI ACGT 2 cut(s) 835, 1027
TaqI TCGA 6 cut(s) 95, 393, 480, 567, 963, 1046
TaqII GACCGA 1 cut(s) 93
TasI AATT 7 cut(s) 465, 668, 903, 1073, 1478, 1547, 1767
TatI WGTACW 1 cut(s) 853
TauI GCSGC 4 cut(s) 45, 116, 119, 157
TfiI GAWTC 7 cut(s) 21, 409, 508, 795, 1186, 1340, 1655
Tru1I TTAA 4 cut(s) 687, 1404, 1749, 1948
Tru9I TTAA 4 cut(s) 687, 1404, 1749, 1948
TscAI CASTG 4 cut(s) 792, 1146, 1881, 1893
TseFI GTSAC 3 cut(s) 1137, 1234, 1511
Tsp45I GTSAC 3 cut(s) 1137, 1234, 1511
TspDTI ATGAA 5 cut(s) 613, 969, 1520, 1560, 1647
TspGWI ACGGA 3 cut(s) 937, 1220, 1826
TspRI CASTG 4 cut(s) 792, 1146, 1881, 1893
VpaK11BI GGWCC 3 cut(s) 48, 364, 592
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 1073
XbaI TCTAGA 2 cut(s) 221, 835
XceI RCATGY 1 cut(s) 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1900
XmnI GAANNNNTTC 2 cut(s) 677, 903
XspI CTAG 7 cut(s) 222, 353, 836, 1239, 1442, 1488, 1590
ZraI GACGTC 1 cut(s) 833
Zsp2I ATGCAT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.