MD04G1205700.v1.1

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009905
Physical Location & Seq
Reverse (-)
29174079 .. 29180917
6839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1205700.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGCCAAAGTTGCAGCAGGAAACGATCACCCTGTTGGCCAAACCGCAGTCCGAATTGTTGTGGCCGGAGACCGTGGCACCGGGAAATCCAGCTTGATTGTGACTGCTGCCACCGAGAACTTCCCGGCAAATGTGCCTCCGGTGCTGCCCCCTACAAGGCTGCCTGAAGATTTCTACCCTGAACGCGTTCCCATCACCCTTATCGACACATCATCTCGAGCAGAGGATACTAATAAAGTTGCTGAAGAGTTGAAACGGGCTGATGCAGTTGTGCTTACTTATGCATGTAATCAGCCTCAGACACTTGATCGATTGAGTACATTTTGGCTTCCAAAACTTCGTCAACTAGAGGTAAAGGTACCTGTTATAGTGGTGGGTTGTAAGCTAGATTTGAGAGATGAGAACCAACAGGTAAGCCTCGAACAAGTGATGTCACCAATAATGCAACAGTTTCGGGAGATTGAAACTTGCATTGAATGTTCAGCATATAAACATATTCAGATTCCGGAGGTTTTCTACTATGCACAAAAAGCTGTGCTTCATCCAACGGGCCCATTATTCGATCAGGAAACACAGACTTTAAAGGACCAATGTGTGCGAGCCTTGAAGCGGATATTCATTCTTTGTGATCATGATAGGGATGGTGCCCTCAGTGATGCAGAGCTGAATGATTTCCAGGTTAAATGTTTCAATGCTCCATTACAACCTTCTGAAATTGTGGGTGTTAAGAGGGTCGTGCAAGAAAAATTACCAGAAGGAGTCAATGAACATGGGCTTACATTGACAGGGTTTCTTTTTCTTCATGCACTATTCATAGAGAAGGGACGTCTAGAGACAACGTGGACTGTCCTTAGGAAGTTTGGATACAATAATGATATAAGGCTTGCAGATGAACTAATCCCATCTCTCCCTAAACGAAGTCCTGATCAGAGTGTGGAGCTGACAAATGAGGCAATTGAGTTTCTGAAGGCTAACTTTGACTTGTTTGGCGGTGATGGTGATGGGGCCCTCCGACCACGTGAACTTGGAGAACTATTTTCTACTGCTCCAGCAAGCCCGTTTAGTGAATTTCCATCTAAGGATGCTGCATTTGGAGGGTTATCACTTGATGGATTTTTTTCACAGTGGGCCCTCATGACACTCTTAAACCCAGCTTCTACTATGGAGAATCTGATATACATTGGTTATCCTGGTGAGGTTTCATCTGCAATCCGTGTGACTAGGAAGAGGCGTGTAGATCGCAAGAAGCAGCAATCGGAAAGAAATGTTTTCCAATGCTTTGTCTTTGGTCCAAAGAAGGCTGGGAAGTCTGCATTATTGGATTCTTTTCTTGGAAGGCCATTTTCTGAAACTTATAATCCAACCACTGAGGAGCGTTATGCGGTTAATGTTGTTGATCAACCTGGGGGACCCAAGAAAACCCTAGTAATGAGAGAAATTCCTGAAGATGGAGTTGAGAAATTTCTCAGAAATAAAGAGGCTTTGGCTGCGTGTGACATAGCTGTGTTTGTTCACGACAGTTCTGATGAGTCATCCTGGAAGAGAGCAACTGAGTTGCTGGTAGAAGTTGCTAGTCATGGTGAGACTACTGGCTTTGAGGTACCTTGCCTCATTGTTGCAGCTAAAGATGATCTGGATGCATTCCCATCGGCCATTCAACATTCTACGAGGGTTAGCCAGGATATGGGAACAGAGGCTCCTATACCTATCAGCGCAAAGTTGGGTGATTTCAACAATGTATTTCGTAAAATTGTAACTGCCGCGGAGCACCCTCACCTGAGCATTCCCGAGACTGAGGCTGGAAGGACCCGCAAGCAATACCATAAGATCCTTAACCGATCTCTAATGTTTATTTCAGTGGGAGCCGCAGTGGCCATGGTTGGAGTGGCGGCTCACCGAGTCTATGCTGCGAGGAGGAATGCCTCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006810 GO:0006839 GO:0006915 GO:0006928 GO:0006996 GO:0007005 GO:0007006 GO:0007017 GO:0007018 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008219 GO:0008637 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010154 GO:0010970 GO:0012501 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0019725 GO:0019867 GO:0022414 GO:0023052 GO:0030154 GO:0030705 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032989 GO:0034643 GO:0035794 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044706 GO:0046902 GO:0046907 GO:0047497 GO:0048316 GO:0048468 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0051704 GO:0051716 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0072384 GO:0090559 GO:0097190 GO:0097345 GO:0098573 GO:0098588 GO:0098805 GO:0099111 GO:1902108 GO:1902110 GO:1902686 GO:1905710
KEGG Pathways
Metabolic & Signaling
ko04137 ko04214 map04137 map04214
Pfam Domains
Protein Families
EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

644

Amino Acids

71.2

Weight (kDa)

5.91

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 18 - 179 1.2e-13 Ras family
EF_assoc_2 PF08356 233 - 317 6e-30 EF hand associated
EF_assoc_1 PF08355 364 - 418 6.6e-20 EF hand associated
Ras PF00071 427 - 574 1.2e-06 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1356
AatII GACGTC 1 cut(s) 829
Acc65I GGTACC 2 cut(s) 358, 1600
AccB1I GGYRCC 4 cut(s) 77, 358, 644, 1600
AccB7I CCANNNNNTGG 1 cut(s) 1684
AccII CGCG 2 cut(s) 186, 1763
AccIII TCCGGA 1 cut(s) 505
AciI CCGC 9 cut(s) 45, 610, 990, 1382, 1761, 1763, 1810, 1866, 1889
AclWI GGATC 1 cut(s) 1822
AcoI YGGCCR 5 cut(s) 3, 37, 63, 1650, 1872
AcsI RAATTY 3 cut(s) 1067, 1437, 1460
AcuI CTGAAG 4 cut(s) 186, 264, 986, 1464
AcvI CACGTG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 826
AfaI GTAC 3 cut(s) 319, 360, 1602
AfiI CCNNNNNNNGG 4 cut(s) 156, 609, 1448, 1684
AflIII ACRYGT 1 cut(s) 184
AgsI TTSAA 7 cut(s) 253, 464, 476, 607, 691, 1658, 1732
AjnI CCWGG 5 cut(s) 675, 1189, 1402, 1535, 1677
AloI GAACNNNNNNTCC 2 cut(s) 1681, 1713
AluBI AGCT 8 cut(s) 93, 385, 533, 664, 940, 1154, 1502, 1622
AluI AGCT 8 cut(s) 93, 385, 533, 664, 940, 1154, 1502, 1622
Alw21I GWGCWC 1 cut(s) 1770
Alw26I GTCTC 4 cut(s) 63, 827, 1577, 1784
AlwI GGATC 1 cut(s) 1822
Ama87I CYCGRG 2 cut(s) 216, 1787
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 9 cut(s) 3, 37, 63, 550, 1005, 1128, 1337, 1650, 1872
ApaI GGGCCC 3 cut(s) 554, 1009, 1132
ApeKI GCWGC 9 cut(s) 14, 107, 145, 160, 1085, 1249, 1487, 1619, 1907
ApoI RAATTY 3 cut(s) 1067, 1437, 1460
Asp700I GAANNNNTTC 2 cut(s) 186, 671
Asp718I GGTACC 2 cut(s) 358, 1600
AspLEI GCGC 1 cut(s) 1715
AsuC2I CCSGG 2 cut(s) 82, 125
AvaI CYCGRG 2 cut(s) 216, 1787
AvaII GGWCC 4 cut(s) 586, 1289, 1409, 1806
AxyI CCTNAGG 1 cut(s) 851
BaeGI GKGCMC 4 cut(s) 554, 649, 1009, 1132
BalI TGGCCA 3 cut(s) 5, 39, 1874
BanI GGYRCC 4 cut(s) 77, 358, 644, 1600
BanII GRGCYC 3 cut(s) 554, 1009, 1132
BarI GAAGNNNNNNTAC 2 cut(s) 848, 880
BbrPI CACGTG 1 cut(s) 1019
Bbv12I GWGCWC 1 cut(s) 1770
BbvI GCAGC 9 cut(s) 26, 94, 132, 147, 1072, 1261, 1474, 1631, 1894
BccI CCATC 9 cut(s) 200, 635, 910, 989, 995, 1081, 1103, 1442, 1654
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 1629, 1663
BciT130I CCWGG 5 cut(s) 677, 1191, 1404, 1537, 1679
BciVI GTATCC 2 cut(s) 220, 857
BclI TGATCA 3 cut(s) 628, 925, 1396
BcnI CCSGG 2 cut(s) 82, 125
BcoDI GTCTC 4 cut(s) 63, 827, 1577, 1784
BfaI CTAG 6 cut(s) 347, 386, 830, 1221, 1424, 1572
BfuI GTATCC 2 cut(s) 220, 857
BglI GCCNNNNNGGC 1 cut(s) 1871
Bme1390I CCNGG 7 cut(s) 82, 125, 677, 1191, 1404, 1537, 1679
Bme18I GGWCC 4 cut(s) 586, 1289, 1409, 1806
BmeT110I CYCGRG 2 cut(s) 216, 1787
BmrFI CCNGG 7 cut(s) 82, 125, 677, 1191, 1404, 1537, 1679
BmsI GCATC 4 cut(s) 253, 646, 1072, 1627
BpmI CTGGAG 1 cut(s) 1032
Bpu10I CCTNAGC 1 cut(s) 1778
BpuMI CCSGG 2 cut(s) 82, 125
Bsa29I ATCGAT 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 1019
BsaHI GRCGYC 1 cut(s) 826
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 73, 1403, 1761, 1875
BsaWI WCCGGW 3 cut(s) 139, 505, 1925
BsaXI ACNNNNNCTCC 4 cut(s) 1681, 1711, 1854, 1884
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 609, 1448, 1684
Bse1I ACTGG 1 cut(s) 1594
Bse21I CCTNAGG 1 cut(s) 851
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 5 cut(s) 677, 1191, 1404, 1537, 1679
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 4 cut(s) 73, 1403, 1761, 1875
BseGI GGATG 5 cut(s) 541, 646, 1087, 1532, 1642
BseLI CCNNNNNNNGG 4 cut(s) 156, 609, 1448, 1684
BseMII CTCAG 7 cut(s) 311, 664, 1359, 1480, 1542, 1769, 1785
BseNI ACTGG 1 cut(s) 1594
BseRI GAGGAG 2 cut(s) 1385, 1927
BseSI GKGCMC 4 cut(s) 554, 649, 1009, 1132
BseXI GCAGC 9 cut(s) 26, 94, 132, 147, 1072, 1261, 1474, 1631, 1894
BseYI CCCAGC 2 cut(s) 1150, 1301
Bsh1236I CGCG 2 cut(s) 186, 1763
BshFI GGCC 9 cut(s) 5, 39, 65, 552, 1007, 1130, 1339, 1652, 1874
BshNI GGYRCC 4 cut(s) 77, 358, 644, 1600
BshVI ATCGAT 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 1770
BsiHKCI CYCGRG 2 cut(s) 216, 1787
BsiSI CCGG 6 cut(s) 66, 81, 125, 140, 506, 1926
BslFI GGGAC 2 cut(s) 837, 1422
BslI CCNNNNNNNGG 4 cut(s) 156, 609, 1448, 1684
BsmAI GTCTC 4 cut(s) 63, 827, 1577, 1784
BsmFI GGGAC 2 cut(s) 837, 1422
BsmI GAATGC 3 cut(s) 1640, 1782, 1924
BsnI GGCC 9 cut(s) 5, 39, 65, 552, 1007, 1130, 1339, 1652, 1874
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 2 cut(s) 216, 1787
Bsp120I GGGCCC 3 cut(s) 550, 1005, 1128
Bsp1286I GDGCHC 5 cut(s) 554, 649, 1009, 1132, 1770
Bsp13I TCCGGA 1 cut(s) 505
Bsp19I CCATGG 1 cut(s) 1875
BspACI CCGC 9 cut(s) 45, 610, 990, 1382, 1761, 1763, 1810, 1866, 1889
BspANI GGCC 9 cut(s) 5, 39, 65, 552, 1007, 1130, 1339, 1652, 1874
BspCNI CTCAG 7 cut(s) 310, 663, 1360, 1479, 1543, 1770, 1786
BspDI ATCGAT 1 cut(s) 310
BspEI TCCGGA 1 cut(s) 505
BspFNI CGCG 2 cut(s) 186, 1763
BspHI TCATGA 2 cut(s) 631, 1134
BspPI GGATC 1 cut(s) 1822
BspT107I GGYRCC 4 cut(s) 77, 358, 644, 1600
BspTNI GGTCTC 1 cut(s) 63
BsrI ACTGG 1 cut(s) 1594
BssECI CCNNGG 4 cut(s) 73, 1403, 1761, 1875
BssNI GRCGYC 1 cut(s) 826
BssT1I CCWWGG 1 cut(s) 1875
Bst2UI CCWGG 5 cut(s) 677, 1191, 1404, 1537, 1679
Bst4CI ACNGT 5 cut(s) 74, 450, 847, 1125, 1520
Bst6I CTCTTC 3 cut(s) 240, 1220, 1535
BstACI GRCGYC 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 4 cut(s) 600, 885, 1054, 1814
BstDEI CTNAG 9 cut(s) 297, 650, 851, 1077, 1368, 1466, 1551, 1778, 1794
BstDSI CCRYGG 3 cut(s) 73, 1761, 1875
BstF5I GGATG 5 cut(s) 541, 646, 1087, 1532, 1642
BstFNI CGCG 2 cut(s) 186, 1763
BstHHI GCGC 1 cut(s) 1715
BstMAI GTCTC 4 cut(s) 63, 827, 1577, 1784
BstMWI GCNNNNNNNGC 5 cut(s) 11, 142, 530, 1487, 1871
BstNI CCWGG 5 cut(s) 677, 1191, 1404, 1537, 1679
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 7 cut(s) 80, 123, 675, 1189, 1402, 1535, 1677
BstSLI GKGCMC 4 cut(s) 554, 649, 1009, 1132
BstUI CGCG 2 cut(s) 186, 1763
BstV1I GCAGC 9 cut(s) 26, 94, 132, 147, 1072, 1261, 1474, 1631, 1894
BstX2I RGATCY 1 cut(s) 1827
BstYI RGATCY 1 cut(s) 1827
Bsu15I ATCGAT 1 cut(s) 310
Bsu36I CCTNAGG 1 cut(s) 851
BsuI GTATCC 2 cut(s) 220, 857
BsuRI GGCC 9 cut(s) 5, 39, 65, 552, 1007, 1130, 1339, 1652, 1874
BsuTUI ATCGAT 1 cut(s) 310
BtgI CCRYGG 3 cut(s) 73, 1761, 1875
BtsCI GGATG 5 cut(s) 541, 646, 1087, 1532, 1642
BtsI GCAGTG 1 cut(s) 1875
BtsIMutI CAGTG 5 cut(s) 658, 1130, 1365, 1863, 1875
Cac8I GCNNGC 4 cut(s) 600, 885, 1054, 1814
CciI TCATGA 2 cut(s) 631, 1134
CfoI GCGC 1 cut(s) 1715
Cfr42I CCGCGG 1 cut(s) 1764
ClaI ATCGAT 1 cut(s) 310
Csp6I GTAC 3 cut(s) 318, 359, 1601
CviAII CATG 7 cut(s) 285, 632, 770, 803, 1135, 1577, 1876
CviQI GTAC 3 cut(s) 318, 359, 1601
DdeI CTNAG 9 cut(s) 297, 650, 851, 1077, 1368, 1466, 1551, 1778, 1794
DraI TTTAAA 1 cut(s) 582
EaeI YGGCCR 5 cut(s) 3, 37, 63, 1650, 1872
Eam1104I CTCTTC 3 cut(s) 240, 1220, 1535
EarI CTCTTC 3 cut(s) 240, 1220, 1535
Eco130I CCWWGG 1 cut(s) 1875
Eco24I GRGCYC 3 cut(s) 554, 1009, 1132
Eco31I GGTCTC 1 cut(s) 63
Eco47I GGWCC 4 cut(s) 586, 1289, 1409, 1806
Eco57I CTGAAG 4 cut(s) 186, 264, 986, 1464
Eco72I CACGTG 1 cut(s) 1019
Eco81I CCTNAGG 1 cut(s) 851
Eco88I CYCGRG 2 cut(s) 216, 1787
EcoO109I RGGNCCY 5 cut(s) 1005, 1006, 1129, 1409, 1806
EcoRII CCWGG 5 cut(s) 675, 1189, 1402, 1535, 1677
EcoT14I CCWWGG 1 cut(s) 1875
EcoT22I ATGCAT 2 cut(s) 286, 1642
EcoT38I GRGCYC 3 cut(s) 554, 1009, 1132
ErhI CCWWGG 1 cut(s) 1875
FaeI CATG 7 cut(s) 288, 635, 773, 806, 1138, 1580, 1879
FalI AAGNNNNNCTT 4 cut(s) 522, 554, 959, 991
FaqI GGGAC 2 cut(s) 837, 1422
FatI CATG 7 cut(s) 284, 631, 769, 802, 1134, 1576, 1875
FauI CCCGC 1 cut(s) 1817
FbaI TGATCA 3 cut(s) 628, 925, 1396
FokI GGATG 5 cut(s) 528, 653, 1094, 1519, 1649
FriOI GRGCYC 3 cut(s) 554, 1009, 1132
FspBI CTAG 6 cut(s) 347, 386, 830, 1221, 1424, 1572
GlaI GCGC 1 cut(s) 1714
GsaI CCCAGC 2 cut(s) 1154, 1305
GsuI CTGGAG 1 cut(s) 1032
HaeIII GGCC 9 cut(s) 5, 39, 65, 552, 1007, 1130, 1339, 1652, 1874
HapII CCGG 6 cut(s) 66, 81, 125, 140, 506, 1926
HhaI GCGC 1 cut(s) 1715
Hin1I GRCGYC 1 cut(s) 826
Hin1II CATG 7 cut(s) 288, 635, 773, 806, 1138, 1580, 1879
Hin6I GCGC 1 cut(s) 1713
HinP1I GCGC 1 cut(s) 1713
HincII GTYRAC 1 cut(s) 344
HindII GTYRAC 1 cut(s) 344
HinfI GANTC 6 cut(s) 502, 759, 1168, 1322, 1529, 1899
HpaII CCGG 6 cut(s) 66, 81, 125, 140, 506, 1926
Hpy166II GTNNAC 4 cut(s) 344, 843, 1022, 1513
Hpy8I GTNNAC 4 cut(s) 344, 843, 1022, 1513
HpyAV CCTTC 7 cut(s) 717, 749, 814, 961, 1291, 1329, 1797
HpyCH4III ACNGT 5 cut(s) 74, 450, 847, 1125, 1520
HpyCH4IV ACGT 3 cut(s) 826, 839, 1018
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 142, 530, 1487, 1871
HpyF3I CTNAG 9 cut(s) 297, 650, 851, 1077, 1368, 1466, 1551, 1778, 1794
HpySE526I ACGT 3 cut(s) 826, 839, 1018
Hsp92I GRCGYC 1 cut(s) 826
Hsp92II CATG 7 cut(s) 288, 635, 773, 806, 1138, 1580, 1879
HspAI GCGC 1 cut(s) 1713
KflI GGGWCCC 1 cut(s) 1409
Kpn2I TCCGGA 1 cut(s) 505
KpnI GGTACC 2 cut(s) 362, 1604
Ksp22I TGATCA 3 cut(s) 628, 925, 1396
KspI CCGCGG 1 cut(s) 1764
LmnI GCTCC 7 cut(s) 700, 937, 1051, 1372, 1702, 1765, 1862
Lsp1109I GCAGC 9 cut(s) 26, 94, 132, 147, 1072, 1261, 1474, 1631, 1894
LweI GCATC 4 cut(s) 253, 646, 1072, 1627
MaeI CTAG 6 cut(s) 347, 386, 830, 1221, 1424, 1572
MaeII ACGT 3 cut(s) 826, 839, 1018
MaeIII GTNAC 5 cut(s) 100, 432, 1216, 1493, 1753
MboII GAAGA 6 cut(s) 179, 257, 791, 1237, 1457, 1552
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 1 cut(s) 1827
MhlI GDGCHC 5 cut(s) 554, 649, 1009, 1132, 1770
MlsI TGGCCA 3 cut(s) 5, 39, 1874
MluCI AATT 8 cut(s) 54, 714, 746, 954, 1067, 1437, 1460, 1749
MluI ACGCGT 1 cut(s) 184
MluNI TGGCCA 3 cut(s) 5, 39, 1874
MlyI GAGTC 3 cut(s) 768, 1538, 1908
MmeI TCCRAC 4 cut(s) 569, 1036, 1385, 1861
Mox20I TGGCCA 3 cut(s) 5, 39, 1874
Mph1103I ATGCAT 2 cut(s) 286, 1642
MroI TCCGGA 1 cut(s) 505
MroXI GAANNNNTTC 2 cut(s) 186, 671
MscI TGGCCA 3 cut(s) 5, 39, 1874
MseI TTAA 7 cut(s) 581, 681, 726, 1145, 1386, 1833, 1930
MslI CAYNNNNRTG 1 cut(s) 1502
Msp20I TGGCCA 3 cut(s) 5, 39, 1874
MspA1I CMGCKG 1 cut(s) 1763
MspI CCGG 6 cut(s) 66, 81, 125, 140, 506, 1926
MspR9I CCNGG 7 cut(s) 82, 125, 677, 1191, 1404, 1537, 1679
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 3 cut(s) 1640, 1782, 1924
MvaI CCWGG 5 cut(s) 677, 1191, 1404, 1537, 1679
MvnI CGCG 2 cut(s) 186, 1763
MwoI GCNNNNNNNGC 5 cut(s) 11, 142, 530, 1487, 1871
NciI CCSGG 2 cut(s) 82, 125
NcoI CCATGG 1 cut(s) 1875
NlaIII CATG 7 cut(s) 288, 635, 773, 806, 1138, 1580, 1879
NmuCI GTSAC 4 cut(s) 100, 432, 1216, 1493
NsiI ATGCAT 2 cut(s) 286, 1642
NspI RCATGY 1 cut(s) 288
PaeR7I CTCGAG 1 cut(s) 216
PagI TCATGA 2 cut(s) 631, 1134
PctI GAATGC 3 cut(s) 1640, 1782, 1924
PdmI GAANNNNTTC 2 cut(s) 186, 671
PfeI GAWTC 3 cut(s) 502, 1168, 1322
PflMI CCANNNNNTGG 1 cut(s) 1684
PfoI TCCNGGA 1 cut(s) 1535
PleI GAGTC 3 cut(s) 767, 1537, 1907
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 3 cut(s) 767, 1537, 1907
Ppu21I YACGTR 1 cut(s) 1019
PpuMI RGGWCCY 2 cut(s) 1409, 1806
PsiI TTATAA 1 cut(s) 1356
Psp5II RGGWCCY 2 cut(s) 1409, 1806
Psp6I CCWGG 5 cut(s) 675, 1189, 1402, 1535, 1677
PspCI CACGTG 1 cut(s) 1019
PspFI CCCAGC 2 cut(s) 1150, 1301
PspGI CCWGG 5 cut(s) 675, 1189, 1402, 1535, 1677
PspOMI GGGCCC 3 cut(s) 550, 1005, 1128
PspPPI RGGWCCY 2 cut(s) 1409, 1806
PsuI RGATCY 1 cut(s) 1827
RsaI GTAC 3 cut(s) 319, 360, 1602
RsaNI GTAC 3 cut(s) 318, 359, 1601
RseI CAYNNNNRTG 1 cut(s) 1502
SacII CCGCGG 1 cut(s) 1764
SaqAI TTAA 7 cut(s) 581, 681, 726, 1145, 1386, 1833, 1930
SchI GAGTC 3 cut(s) 768, 1538, 1908
ScrFI CCNGG 7 cut(s) 82, 125, 677, 1191, 1404, 1537, 1679
SduI GDGCHC 5 cut(s) 554, 649, 1009, 1132, 1770
SfaNI GCATC 4 cut(s) 253, 646, 1072, 1627
Sfr274I CTCGAG 1 cut(s) 216
Sfr303I CCGCGG 1 cut(s) 1764
SgrBI CCGCGG 1 cut(s) 1764
SinI GGWCC 4 cut(s) 586, 1289, 1409, 1806
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 1502
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
Sse9I AATT 8 cut(s) 54, 714, 746, 954, 1067, 1437, 1460, 1749
SsiI CCGC 9 cut(s) 45, 610, 990, 1382, 1761, 1763, 1810, 1866, 1889
SspMI CTAG 6 cut(s) 347, 386, 830, 1221, 1424, 1572
StyD4I CCNGG 7 cut(s) 80, 123, 675, 1189, 1402, 1535, 1677
StyI CCWWGG 1 cut(s) 1875
TaaI ACNGT 5 cut(s) 74, 450, 847, 1125, 1520
TaiI ACGT 3 cut(s) 829, 842, 1021
TaqI TCGA 5 cut(s) 204, 217, 310, 420, 561
TasI AATT 8 cut(s) 54, 714, 746, 954, 1067, 1437, 1460, 1749
TatI WGTACW 1 cut(s) 317
TauI GCSGC 3 cut(s) 1763, 1868, 1892
TfiI GAWTC 3 cut(s) 502, 1168, 1322
Tru1I TTAA 7 cut(s) 581, 681, 726, 1145, 1386, 1833, 1930
Tru9I TTAA 7 cut(s) 581, 681, 726, 1145, 1386, 1833, 1930
TscAI CASTG 5 cut(s) 658, 1130, 1372, 1863, 1875
TseFI GTSAC 4 cut(s) 100, 432, 1216, 1493
TseI GCWGC 9 cut(s) 14, 107, 145, 160, 1085, 1249, 1487, 1619, 1907
Tsp45I GTSAC 4 cut(s) 100, 432, 1216, 1493
TspDTI ATGAA 7 cut(s) 530, 607, 780, 791, 802, 906, 1191
TspGWI ACGGA 1 cut(s) 1202
TspRI CASTG 5 cut(s) 658, 1130, 1372, 1863, 1875
Van91I CCANNNNNTGG 1 cut(s) 1684
VpaK11BI GGWCC 4 cut(s) 586, 1289, 1409, 1806
XapI RAATTY 3 cut(s) 1067, 1437, 1460
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 1 cut(s) 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 1882
XhoI CTCGAG 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 186, 671
XspI CTAG 6 cut(s) 347, 386, 830, 1221, 1424, 1572
ZraI GACGTC 1 cut(s) 827
Zsp2I ATGCAT 2 cut(s) 286, 1642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.