MD12G1219900.v1.1

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009905
Physical Location & Seq
Reverse (-)
29682302 .. 29689805
7504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1219900.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGGCCAAAGTTGCAGCAGGAAATGATCACCCTGTTGGCCAAACTGCAGTCCGAATTGTTGTGGCTGGAGACCGCGGCACGGGGAAATCCAGCTTGATTGTGACTGCTGCCACCGAGAACTTTCCGGCAAATGTGCCTCCGGTGCTGCCCCCAACAAGGCTGCCTGAAGATTTCTACCCTGAACGCGTGCCCATCACCATTATCGACACATCTTCTCGACCCGAGGATACCAATAAAGTTGCTGAAGAATTGAAGCGGGCTGATGCAGTCGTGCTTACTTATGCGTGTAATCAGCCCCAGACACTTGATCGGTTGAGTACCTTTTGGCTTCCAAAACTTCGTCAACTAGAGGTAAAGGTACCTGTTATAGTGGTGGGTTGTAAGCTGGATTTGAGAGATGAGAACCAACAGGTGAGCCTCGAACAAGTGATGTCACCAATAATGCAACAGTTTCGGGAGATTGAAACTTGCATTGAATGTTCAGCATATAAACATATTCAGATTCCGGAAGTTTTTTACTATGCACAAAAAGCTGTGCTGCATCCAACGGGCCCATTATTTGATCAGGAAACACAGACTTTAAAGGACCAATGTGTGCGAGCCTTGAAGCGGATATTCATTCTTTGTGATCATGATAGGGATGGTGCCCTCAGTGATGCAGAGTTGAATGATTTCCAGGTTAAATGTTTCAATGCTCCATTACAACCTTCTGAAATAGTGGGTGTTAAGAGGGTTGTGCAAGAAAAATTACCAGAAGGAGTCAATGAACATGGGCTTACATTGACAGGTTTTCTTTTCCTTCATGCACTATTCATAGAGAAGGGACGTCTAGAGACAACGTGGACTGTCCTTAGGAAGTTTGGATACAACAATGAAATAAGACTTGCAGATGAACTAATCCCATCTCCCACTAAACGAACTCCTGACCAGAGTGTGGAGCTGACAAATGAGGCAATCGAGTTTCTGAAGGCTAACTTTGACTTGTATGACAATGATGGTGATGGGGCCCTCCGACCACGTGAACTTGAAGAACTATTTTCTACTGCTCCAGCAAGCCCATTTAGTGAACATCCATATAAGGATGCTGCAGAGAAAAATGCATTTGGAGGGTTATCACTTGATGGATTTTTGTCACAGTGGGCCCTCATGACACTCCTAAACCCAGCTTCTACCATGGAGAATCTGATATACATTGGTTATCCTGGTGATGTTTCATCCACAATCCGTGTGACTAGAAAGAGACGTTTAGATCGCAAGAAACAGCAATCGGAAAGAAATGTTTTCCAATGCTTTGTCTTTGGTCCAAAGAAGGCTGGGAAATCTGCATTATTGGATTCTTTTCTTGGAAGGCCATTTTCTGATACTTATAATCCCACCACTGGGGAGCGTTATGCGGCTAATGTTGTTGATCAACCCGGGGGACCCAAGAAAACACTAGTAATGAGAGAAATTCCTGAAGATGGAGTTGAGAAATTTCTCAGAAATAAAGAGGCTTTGGCTGCATGTGACATAGCTGTGTTTGTTCACGACAGTTCTGATGAGTCGTCCTGGAGGAGAGCAACTGAGTTGCTGATAGAAGTTGCTAGTCATGGTGAGACTACTGGCTTTGAGGTACCTTGCCTCATTGTTGCAGCTAAAGATGATCTGGATGCATTCCCATCGGCCATTCAACATTCTACGAGGGTTAGCCAGGATATGGGAACAGAGGCTCCTATACCTATCAGCACAAAGTTGGGTGATTTCAACAATGTATTTCGTAAAATTGTAACTGCCGCGGAACACCCTCATCTGAGCATTCCTGAGACTGAGGCTGGAAAGACCCGCAAGCAATACCATAAGATCCTTAACCGATCTCTTATGTTTATTTCAGTGGGAGCTGCAGTGGCCATGGTTGGAGTGGCGGCTTACCGAGTCTATGCAGCGAGGAAGAACGCCTCCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006810 GO:0006839 GO:0006915 GO:0006928 GO:0006996 GO:0007005 GO:0007006 GO:0007017 GO:0007018 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008219 GO:0008637 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010154 GO:0010970 GO:0012501 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0019725 GO:0019867 GO:0022414 GO:0023052 GO:0030154 GO:0030705 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032989 GO:0034643 GO:0035794 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044706 GO:0046902 GO:0046907 GO:0047497 GO:0048316 GO:0048468 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0051704 GO:0051716 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0072384 GO:0090559 GO:0097190 GO:0097345 GO:0098573 GO:0098588 GO:0098805 GO:0099111 GO:1902108 GO:1902110 GO:1902686 GO:1905710
KEGG Pathways
Metabolic & Signaling
ko04137 ko04214 map04137 map04214
Pfam Domains
Protein Families
EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

648

Amino Acids

71.91

Weight (kDa)

5.83

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 18 - 179 6.7e-14 Ras family
EF_assoc_2 PF08356 233 - 317 1.9e-30 EF hand associated
EF_assoc_1 PF08355 356 - 422 1.1e-22 EF hand associated
Ras PF00071 431 - 559 4.8e-06 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1368
AatII GACGTC 1 cut(s) 829
Acc65I GGTACC 2 cut(s) 358, 1612
AccB1I GGYRCC 3 cut(s) 358, 644, 1612
AccB7I CCANNNNNTGG 2 cut(s) 934, 1696
AccII CGCG 3 cut(s) 75, 186, 1775
AccIII TCCGGA 1 cut(s) 505
AciI CCGC 9 cut(s) 73, 75, 256, 610, 1394, 1773, 1775, 1822, 1901
AclWI GGATC 1 cut(s) 1834
AcoI YGGCCR 4 cut(s) 3, 37, 1662, 1884
AcsI RAATTY 2 cut(s) 1449, 1472
AcuI CTGAAG 4 cut(s) 186, 264, 986, 1476
AcvI CACGTG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 826
AfaI GTAC 3 cut(s) 319, 360, 1614
AfiI CCNNNNNNNGG 8 cut(s) 79, 156, 609, 934, 1379, 1380, 1460, 1696
AflIII ACRYGT 1 cut(s) 184
AgsI TTSAA 9 cut(s) 253, 464, 476, 607, 667, 691, 1028, 1670, 1744
AhlI ACTAGT 1 cut(s) 1435
AjnI CCWGG 4 cut(s) 675, 1201, 1547, 1689
AloI GAACNNNNNNTCC 2 cut(s) 1693, 1725
AluBI AGCT 8 cut(s) 93, 385, 533, 940, 1166, 1514, 1634, 1877
AluI AGCT 8 cut(s) 93, 385, 533, 940, 1166, 1514, 1634, 1877
Alw26I GTCTC 5 cut(s) 63, 827, 1234, 1589, 1796
AlwI GGATC 1 cut(s) 1834
Ama87I CYCGRG 2 cut(s) 221, 1415
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 8 cut(s) 3, 37, 550, 1005, 1140, 1349, 1662, 1884
ApaI GGGCCC 3 cut(s) 554, 1009, 1144
ApoI RAATTY 2 cut(s) 1449, 1472
Asp700I GAANNNNTTC 1 cut(s) 671
Asp718I GGTACC 2 cut(s) 358, 1612
AspS9I GGNCC 9 cut(s) 550, 551, 586, 1005, 1006, 1140, 1141, 1301, 1421
AsuC2I CCSGG 2 cut(s) 1416, 1417
AsuHPI GGTGA 8 cut(s) 20, 187, 424, 426, 1010, 1217, 1604, 1748
AvaI CYCGRG 2 cut(s) 221, 1415
AvaII GGWCC 3 cut(s) 586, 1301, 1421
AxyI CCTNAGG 1 cut(s) 851
BaeGI GKGCMC 5 cut(s) 192, 554, 649, 1009, 1144
BalI TGGCCA 3 cut(s) 5, 39, 1886
BanI GGYRCC 3 cut(s) 358, 644, 1612
BanII GRGCYC 3 cut(s) 554, 1009, 1144
BarI GAAGNNNNNNTAC 2 cut(s) 848, 880
BbrPI CACGTG 1 cut(s) 1019
BccI CCATC 8 cut(s) 200, 635, 910, 989, 995, 1115, 1454, 1666
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 1641, 1675
BciT130I CCWGG 4 cut(s) 677, 1203, 1549, 1691
BciVI GTATCC 2 cut(s) 220, 857
BclI TGATCA 4 cut(s) 25, 562, 628, 1408
BcnI CCSGG 2 cut(s) 1416, 1417
BcoDI GTCTC 5 cut(s) 63, 827, 1234, 1589, 1796
BcuI ACTAGT 1 cut(s) 1435
BfaI CTAG 5 cut(s) 347, 830, 1233, 1436, 1584
BfmI CTRYAG 3 cut(s) 45, 1086, 1878
BfuI GTATCC 2 cut(s) 220, 857
Bme1390I CCNGG 6 cut(s) 677, 1203, 1416, 1417, 1549, 1691
Bme18I GGWCC 3 cut(s) 586, 1301, 1421
BmeT110I CYCGRG 2 cut(s) 221, 1415
BmgT120I GGNCC 9 cut(s) 550, 551, 586, 1005, 1006, 1140, 1141, 1301, 1421
BmrFI CCNGG 6 cut(s) 677, 1203, 1416, 1417, 1549, 1691
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 5 cut(s) 253, 550, 646, 1072, 1639
BmuI ACTGGG 1 cut(s) 1389
BpmI CTGGAG 4 cut(s) 87, 1032, 1570, 1921
BpuMI CCSGG 2 cut(s) 1416, 1417
BsaAI YACGTR 1 cut(s) 1019
BsaHI GRCGYC 1 cut(s) 826
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 7 cut(s) 73, 222, 1173, 1415, 1416, 1773, 1887
BsaWI WCCGGW 2 cut(s) 139, 505
BsaXI ACNNNNNCTCC 4 cut(s) 1693, 1723, 1866, 1896
Bsc4I CCNNNNNNNGG 8 cut(s) 79, 156, 609, 934, 1379, 1380, 1460, 1696
Bse1I ACTGG 3 cut(s) 1384, 1606, 1938
Bse21I CCTNAGG 1 cut(s) 851
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 4 cut(s) 677, 1203, 1549, 1691
BseDI CCNNGG 7 cut(s) 73, 222, 1173, 1415, 1416, 1773, 1887
BseGI GGATG 6 cut(s) 541, 646, 1069, 1087, 1214, 1654
BseLI CCNNNNNNNGG 8 cut(s) 79, 156, 609, 934, 1379, 1380, 1460, 1696
BseMII CTCAG 6 cut(s) 664, 1492, 1554, 1781, 1791, 1797
BseNI ACTGG 3 cut(s) 1384, 1606, 1938
BseRI GAGGAG 1 cut(s) 1567
BseSI GKGCMC 5 cut(s) 192, 554, 649, 1009, 1144
BseYI CCCAGC 2 cut(s) 1162, 1313
Bsh1236I CGCG 3 cut(s) 75, 186, 1775
BshFI GGCC 8 cut(s) 5, 39, 552, 1007, 1142, 1351, 1664, 1886
BshNI GGYRCC 3 cut(s) 358, 644, 1612
BsiHKCI CYCGRG 2 cut(s) 221, 1415
BsiSI CCGG 4 cut(s) 125, 140, 506, 1416
BslFI GGGAC 2 cut(s) 837, 1434
BslI CCNNNNNNNGG 8 cut(s) 79, 156, 609, 934, 1379, 1380, 1460, 1696
BsmAI GTCTC 5 cut(s) 63, 827, 1234, 1589, 1796
BsmBI CGTCTC 1 cut(s) 1234
BsmFI GGGAC 2 cut(s) 837, 1434
BsmI GAATGC 2 cut(s) 1652, 1794
BsnI GGCC 8 cut(s) 5, 39, 552, 1007, 1142, 1351, 1664, 1886
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 2 cut(s) 221, 1415
Bsp120I GGGCCC 3 cut(s) 550, 1005, 1140
Bsp1286I GDGCHC 5 cut(s) 192, 554, 649, 1009, 1144
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
Bsp19I CCATGG 2 cut(s) 1173, 1887
BspACI CCGC 9 cut(s) 73, 75, 256, 610, 1394, 1773, 1775, 1822, 1901
BspANI GGCC 8 cut(s) 5, 39, 552, 1007, 1142, 1351, 1664, 1886
BspCNI CTCAG 6 cut(s) 663, 1491, 1555, 1782, 1792, 1798
BspEI TCCGGA 1 cut(s) 505
BspFNI CGCG 3 cut(s) 75, 186, 1775
BspHI TCATGA 2 cut(s) 631, 1146
BspMAI CTGCAG 3 cut(s) 49, 1090, 1882
BspPI GGATC 1 cut(s) 1834
BspT107I GGYRCC 3 cut(s) 358, 644, 1612
BspTNI GGTCTC 1 cut(s) 63
BsrI ACTGG 3 cut(s) 1384, 1606, 1938
BssECI CCNNGG 7 cut(s) 73, 222, 1173, 1415, 1416, 1773, 1887
BssMI GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
BssNI GRCGYC 1 cut(s) 826
BssT1I CCWWGG 2 cut(s) 1173, 1887
Bst2UI CCWGG 4 cut(s) 677, 1203, 1549, 1691
Bst4CI ACNGT 4 cut(s) 450, 847, 1137, 1532
BstACI GRCGYC 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 5 cut(s) 188, 258, 600, 1054, 1826
BstDEI CTNAG 7 cut(s) 650, 851, 1478, 1563, 1790, 1800, 1806
BstDSI CCRYGG 4 cut(s) 73, 1173, 1773, 1887
BstF5I GGATG 6 cut(s) 541, 646, 1069, 1087, 1214, 1654
BstFNI CGCG 3 cut(s) 75, 186, 1775
BstKTI GATC 9 cut(s) 28, 310, 565, 631, 1252, 1411, 1645, 1842, 1853
BstMAI GTCTC 5 cut(s) 63, 827, 1234, 1589, 1796
BstMBI GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
BstMWI GCNNNNNNNGC 5 cut(s) 11, 142, 530, 1499, 1883
BstNI CCWGG 4 cut(s) 677, 1203, 1549, 1691
BstNSI RCATGY 1 cut(s) 1506
BstSCI CCNGG 6 cut(s) 675, 1201, 1414, 1415, 1547, 1689
BstSFI CTRYAG 3 cut(s) 45, 1086, 1878
BstSLI GKGCMC 5 cut(s) 192, 554, 649, 1009, 1144
BstUI CGCG 3 cut(s) 75, 186, 1775
BstX2I RGATCY 1 cut(s) 1839
BstYI RGATCY 1 cut(s) 1839
Bsu36I CCTNAGG 1 cut(s) 851
BsuI GTATCC 2 cut(s) 220, 857
BsuRI GGCC 8 cut(s) 5, 39, 552, 1007, 1142, 1351, 1664, 1886
BtgI CCRYGG 4 cut(s) 73, 1173, 1773, 1887
BtsCI GGATG 6 cut(s) 541, 646, 1069, 1087, 1214, 1654
BtsI GCAGTG 1 cut(s) 1887
BtsIMutI CAGTG 5 cut(s) 658, 1142, 1377, 1875, 1887
Cac8I GCNNGC 5 cut(s) 188, 258, 600, 1054, 1826
CciI TCATGA 2 cut(s) 631, 1146
Cfr13I GGNCC 9 cut(s) 550, 551, 586, 1005, 1006, 1140, 1141, 1301, 1421
Cfr42I CCGCGG 2 cut(s) 76, 1776
Cfr9I CCCGGG 1 cut(s) 1415
Csp6I GTAC 3 cut(s) 318, 359, 1613
CviAII CATG 8 cut(s) 632, 770, 803, 1147, 1174, 1503, 1589, 1888
CviQI GTAC 3 cut(s) 318, 359, 1613
DdeI CTNAG 7 cut(s) 650, 851, 1478, 1563, 1790, 1800, 1806
DpnI GATC 9 cut(s) 27, 309, 564, 630, 1251, 1410, 1644, 1841, 1852
DpnII GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
DraI TTTAAA 1 cut(s) 582
EaeI YGGCCR 4 cut(s) 3, 37, 1662, 1884
Eco130I CCWWGG 2 cut(s) 1173, 1887
Eco24I GRGCYC 3 cut(s) 554, 1009, 1144
Eco31I GGTCTC 1 cut(s) 63
Eco47I GGWCC 3 cut(s) 586, 1301, 1421
Eco57I CTGAAG 4 cut(s) 186, 264, 986, 1476
Eco72I CACGTG 1 cut(s) 1019
Eco81I CCTNAGG 1 cut(s) 851
Eco88I CYCGRG 2 cut(s) 221, 1415
EcoO109I RGGNCCY 4 cut(s) 1005, 1006, 1141, 1421
EcoRII CCWGG 4 cut(s) 675, 1201, 1547, 1689
EcoT14I CCWWGG 2 cut(s) 1173, 1887
EcoT22I ATGCAT 2 cut(s) 1102, 1654
EcoT38I GRGCYC 3 cut(s) 554, 1009, 1144
ErhI CCWWGG 2 cut(s) 1173, 1887
Esp3I CGTCTC 1 cut(s) 1234
FaeI CATG 8 cut(s) 635, 773, 806, 1150, 1177, 1506, 1592, 1891
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FaqI GGGAC 2 cut(s) 837, 1434
FatI CATG 8 cut(s) 631, 769, 802, 1146, 1173, 1502, 1588, 1887
FauI CCCGC 2 cut(s) 249, 1829
FbaI TGATCA 4 cut(s) 25, 562, 628, 1408
FokI GGATG 6 cut(s) 528, 653, 1056, 1094, 1201, 1661
FriOI GRGCYC 3 cut(s) 554, 1009, 1144
FspBI CTAG 5 cut(s) 347, 830, 1233, 1436, 1584
GsaI CCCAGC 2 cut(s) 1166, 1317
GsuI CTGGAG 4 cut(s) 87, 1032, 1570, 1921
HaeIII GGCC 8 cut(s) 5, 39, 552, 1007, 1142, 1351, 1664, 1886
HapII CCGG 4 cut(s) 125, 140, 506, 1416
Hin1I GRCGYC 1 cut(s) 826
Hin1II CATG 8 cut(s) 635, 773, 806, 1150, 1177, 1506, 1592, 1891
HincII GTYRAC 1 cut(s) 344
HindII GTYRAC 1 cut(s) 344
HinfI GANTC 6 cut(s) 502, 759, 1180, 1334, 1541, 1911
HpaII CCGG 4 cut(s) 125, 140, 506, 1416
HphI GGTGA 8 cut(s) 20, 187, 424, 426, 1010, 1217, 1604, 1748
Hpy166II GTNNAC 5 cut(s) 344, 843, 1022, 1067, 1525
Hpy8I GTNNAC 5 cut(s) 344, 843, 1022, 1067, 1525
HpyAV CCTTC 7 cut(s) 717, 749, 809, 814, 961, 1303, 1341
HpyCH4III ACNGT 4 cut(s) 450, 847, 1137, 1532
HpyCH4IV ACGT 4 cut(s) 826, 839, 1018, 1243
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 142, 530, 1499, 1883
HpyF3I CTNAG 7 cut(s) 650, 851, 1478, 1563, 1790, 1800, 1806
HpySE526I ACGT 4 cut(s) 826, 839, 1018, 1243
Hsp92I GRCGYC 1 cut(s) 826
Hsp92II CATG 8 cut(s) 635, 773, 806, 1150, 1177, 1506, 1592, 1891
KflI GGGWCCC 1 cut(s) 1421
Kpn2I TCCGGA 1 cut(s) 505
KpnI GGTACC 2 cut(s) 362, 1616
Ksp22I TGATCA 4 cut(s) 25, 562, 628, 1408
KspI CCGCGG 2 cut(s) 76, 1776
Kzo9I GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
LmnI GCTCC 6 cut(s) 700, 937, 1051, 1384, 1714, 1874
LweI GCATC 5 cut(s) 253, 550, 646, 1072, 1639
MaeI CTAG 5 cut(s) 347, 830, 1233, 1436, 1584
MaeII ACGT 4 cut(s) 826, 839, 1018, 1243
MaeIII GTNAC 6 cut(s) 100, 432, 1131, 1228, 1505, 1765
MalI GATC 9 cut(s) 27, 309, 564, 630, 1251, 1410, 1644, 1841, 1852
MboI GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
MboII GAAGA 6 cut(s) 179, 204, 257, 1040, 1469, 1939
MflI RGATCY 1 cut(s) 1839
MhlI GDGCHC 5 cut(s) 192, 554, 649, 1009, 1144
MlsI TGGCCA 3 cut(s) 5, 39, 1886
MluCI AATT 6 cut(s) 54, 248, 746, 1449, 1472, 1761
MluI ACGCGT 1 cut(s) 184
MluNI TGGCCA 3 cut(s) 5, 39, 1886
MlyI GAGTC 3 cut(s) 768, 1550, 1920
MmeI TCCRAC 3 cut(s) 569, 1036, 1873
Mox20I TGGCCA 3 cut(s) 5, 39, 1886
Mph1103I ATGCAT 2 cut(s) 1102, 1654
MroI TCCGGA 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 671
MscI TGGCCA 3 cut(s) 5, 39, 1886
MseI TTAA 5 cut(s) 581, 681, 726, 1845, 1942
MslI CAYNNNNRTG 1 cut(s) 1514
Msp20I TGGCCA 3 cut(s) 5, 39, 1886
MspA1I CMGCKG 2 cut(s) 75, 1775
MspI CCGG 4 cut(s) 125, 140, 506, 1416
MspR9I CCNGG 6 cut(s) 677, 1203, 1416, 1417, 1549, 1691
Mva1269I GAATGC 2 cut(s) 1652, 1794
MvaI CCWGG 4 cut(s) 677, 1203, 1549, 1691
MvnI CGCG 3 cut(s) 75, 186, 1775
MwoI GCNNNNNNNGC 5 cut(s) 11, 142, 530, 1499, 1883
NciI CCSGG 2 cut(s) 1416, 1417
NcoI CCATGG 2 cut(s) 1173, 1887
NdeII GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
NlaIII CATG 8 cut(s) 635, 773, 806, 1150, 1177, 1506, 1592, 1891
NmuCI GTSAC 5 cut(s) 100, 432, 1131, 1228, 1505
NsiI ATGCAT 2 cut(s) 1102, 1654
NspI RCATGY 1 cut(s) 1506
PagI TCATGA 2 cut(s) 631, 1146
PctI GAATGC 2 cut(s) 1652, 1794
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 3 cut(s) 502, 1180, 1334
PflMI CCANNNNNTGG 2 cut(s) 934, 1696
PfoI TCCNGGA 1 cut(s) 1547
PleI GAGTC 3 cut(s) 767, 1549, 1919
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 3 cut(s) 767, 1549, 1919
Ppu21I YACGTR 1 cut(s) 1019
PpuMI RGGWCCY 1 cut(s) 1421
PsiI TTATAA 1 cut(s) 1368
Psp5II RGGWCCY 1 cut(s) 1421
Psp6I CCWGG 4 cut(s) 675, 1201, 1547, 1689
PspCI CACGTG 1 cut(s) 1019
PspFI CCCAGC 2 cut(s) 1162, 1313
PspGI CCWGG 4 cut(s) 675, 1201, 1547, 1689
PspOMI GGGCCC 3 cut(s) 550, 1005, 1140
PspPI GGNCC 9 cut(s) 550, 551, 586, 1005, 1006, 1140, 1141, 1301, 1421
PspPPI RGGWCCY 1 cut(s) 1421
PstI CTGCAG 3 cut(s) 49, 1090, 1882
PsuI RGATCY 1 cut(s) 1839
RsaI GTAC 3 cut(s) 319, 360, 1614
RsaNI GTAC 3 cut(s) 318, 359, 1613
RseI CAYNNNNRTG 1 cut(s) 1514
SacII CCGCGG 2 cut(s) 76, 1776
SaqAI TTAA 5 cut(s) 581, 681, 726, 1845, 1942
Sau3AI GATC 9 cut(s) 25, 307, 562, 628, 1249, 1408, 1642, 1839, 1850
Sau96I GGNCC 9 cut(s) 550, 551, 586, 1005, 1006, 1140, 1141, 1301, 1421
SchI GAGTC 3 cut(s) 768, 1550, 1920
ScrFI CCNGG 6 cut(s) 677, 1203, 1416, 1417, 1549, 1691
SduI GDGCHC 5 cut(s) 192, 554, 649, 1009, 1144
SfaNI GCATC 5 cut(s) 253, 550, 646, 1072, 1639
SfcI CTRYAG 3 cut(s) 45, 1086, 1878
Sfr303I CCGCGG 2 cut(s) 76, 1776
SgrBI CCGCGG 2 cut(s) 76, 1776
SinI GGWCC 3 cut(s) 586, 1301, 1421
SmaI CCCGGG 1 cut(s) 1417
SmiMI CAYNNNNRTG 1 cut(s) 1514
SpeI ACTAGT 1 cut(s) 1435
Sse9I AATT 6 cut(s) 54, 248, 746, 1449, 1472, 1761
SsiI CCGC 9 cut(s) 73, 75, 256, 610, 1394, 1773, 1775, 1822, 1901
SspMI CTAG 5 cut(s) 347, 830, 1233, 1436, 1584
StyD4I CCNGG 6 cut(s) 675, 1201, 1414, 1415, 1547, 1689
StyI CCWWGG 2 cut(s) 1173, 1887
TaaI ACNGT 4 cut(s) 450, 847, 1137, 1532
TaiI ACGT 4 cut(s) 829, 842, 1021, 1246
TaqI TCGA 4 cut(s) 204, 217, 420, 957
TasI AATT 6 cut(s) 54, 248, 746, 1449, 1472, 1761
TauI GCSGC 4 cut(s) 78, 1397, 1775, 1904
TfiI GAWTC 3 cut(s) 502, 1180, 1334
Tru1I TTAA 5 cut(s) 581, 681, 726, 1845, 1942
Tru9I TTAA 5 cut(s) 581, 681, 726, 1845, 1942
TscAI CASTG 5 cut(s) 658, 1142, 1384, 1875, 1887
TseFI GTSAC 5 cut(s) 100, 432, 1131, 1228, 1505
Tsp45I GTSAC 5 cut(s) 100, 432, 1131, 1228, 1505
TspDTI ATGAA 7 cut(s) 607, 780, 791, 802, 888, 906, 1203
TspGWI ACGGA 1 cut(s) 1214
TspMI CCCGGG 1 cut(s) 1415
TspRI CASTG 5 cut(s) 658, 1142, 1384, 1875, 1887
Van91I CCANNNNNTGG 2 cut(s) 934, 1696
VpaK11BI GGWCC 3 cut(s) 586, 1301, 1421
XapI RAATTY 2 cut(s) 1449, 1472
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 1 cut(s) 1506
XcmI CCANNNNNNNNNTGG 1 cut(s) 1894
XmaI CCCGGG 1 cut(s) 1415
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 5 cut(s) 347, 830, 1233, 1436, 1584
ZraI GACGTC 1 cut(s) 827
Zsp2I ATGCAT 2 cut(s) 1102, 1654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.