pycom04g18200

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009905
Physical Location & Seq
Reverse (-)
20313482 .. 20320192
6711 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18200.4

Sequence Viewer

Length: 2121 bp
ATGAAACGACATATGGACACGCGTACCGCCATATCGAAACTTCTAGCAGCCACAGCAACAGCTGCCGCCGCCACCGCCCCCGCCGATCAGCTAGGGCTCTGGTGGGTTTTATCGAATCTTCTCCAACGAAGAAAGAGTTCTCAAAGCCCCCAGCCTAATCCCCATTACTTAGTTTCAATGGCCAAAGTTCCAGCAGGAAACGATCACCCTGTTGGCCAAACCGCAGTCCGAATTGTTGTGGCCGGAGACCATGGCACCGGGAAATCCAGCTTGATTGTGACTGCTGCCACCGAGAACTTCCCGGCAAATGTGCCTCCGGTGCTGCCCCCTACAAGGCTGCCTGAAGATTTCTACCCTGAACGCGTTCCCATCACCATTATCGACACATCATCTCGAGCAGAGGATACTAATAAAGTTGCTGAAGAGTTGAAACGGGCTGATGCAGTTGTGCTTACTTATGCATGTAATCAGCCTCAGACACTTGATCGATTGAGTACATTTTGGCTTCCAAAACTTCGTCAACTAGAGGTAAAGGTACCTGTTATAGTGGTGGGTTGTAAGCTAGATTTGAGAGATGAGAACCAACAGGTGAGCCTCGAACAAGTGATGTCACCAATAATGCAACAGTTTCGGGAGATTGAAACTTGCATCGAATGTTCAGCATATAAACATATTCAGATTCCGGAGGTTTTCTACTATGCACAAAAAGCTGTGCTTCATCCAACGGGCCCATTATTCGATCAGGAAACACAGACTTTGAAGGACCAATGTGTGCGAGCCTTGAAGCGGATATTCATTCTTTGTGATCATGATAGGGATGGTGCCCTCAGTGATGCAGAGCTGAATGATTTCCAGGTTAAATGTTTCAATGCTCCATTACAACCTTCTGAAATTGTGGGTGTTAAGAGGGTCGTGCAAGAAAAATTACCAGAAGGAGTCAATGAACATGGGCTTACATTGACAGGGTTTCTTTTTCTTCATGCACTATTCATAGAGAAGGGACGTCTAGAGACAACGTGGACTGTCCTGAGGAAGTTTGGATACAATAATGATATAAGGCTTGCAGATGAATTAATCCCATCTCTCCCTAAACGAAGTCCTGATCAGAGTGTGGAGCTGACAAATGAGGCAATTGAGTTTCTGAAGGCTAACTTTGACTTGTTTGACGGTGATGGTGATGGGGCCCTCCGACCACGTGAACTTGAAGAACTATTTTCTACTGCTCCAGCAAGCCCGTTTAGTGAATTTCCATCTAAGGATGCTGCATTTGGAGGGTTATCACTTGATGGATTTTTGTCACAGTGGGCCCTCATGACACTCCTAAACCCAGCTTCTACGATGGAGAATCTGATATACATTGGTTATCCTGGTGAGGTTTCATCCGCAATCCGTGTGACTAGGAAGAAGCGTGTAGATCGCAAGAAGCAGCAATCGGAAAGAAATGTTTTCCAATGCTTTGTCTTTGGTCCAAAGAAGGCTGGGAAGTCTGCATTATTGGATTCTTTTCTTGGAAGGCCATTTGCTGAAACTTATAATCCAACCACTGAGGAGCGTTATGCGGTTAGTGTTGTTGATCAACCTGGGGGACCCAAGAAAACCCTAGTAATGAGAGAAATTCCTGAAGATGGAGTTGAGAAATTTCTCAGAAATAAAGAGGCTTTGGCTGCATGTGACATAGCTGTGTTTGTTCACGACAGTTCTGATGAGTCATCCTGGAAGAGAGCAACTGAGTTGCTGGTAGAAGTTGCTAGTCATGGTGAGACTACTGGCTTTGAGGTACCTTGCCTCATTGTTTCAGCTAAAGATGATCTGGTTGCATTCCCATCGGCCATTCAACATTCTACGAGGGTTAGCCAGGATATGGGAACAGAGGCTCCTATACCTATCAGCGCAAAGTTGGGTGATTTCAACAATGTGTTTCGTAAAATTGTAACTGCCGCAGAGCACCCTCACCTGGGCATTCCTGAGACTGAGGCTGGAAGGACCCGCAAGCAATACCATAAGATCGATCTTAACCTGATCTCTAATGTTTATTTCAGTGGGAGCTGCAGTGGCCATGGTTGGAGTGGCGGCTTACCGAGTCTATGCTGCGAGGAGGAACGCCTCCGGTTAAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006810 GO:0006839 GO:0006915 GO:0006928 GO:0006996 GO:0007005 GO:0007006 GO:0007017 GO:0007018 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008219 GO:0008637 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010154 GO:0010970 GO:0012501 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0019725 GO:0019867 GO:0022414 GO:0023052 GO:0030154 GO:0030705 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032989 GO:0034643 GO:0035794 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044706 GO:0046902 GO:0046907 GO:0047497 GO:0048316 GO:0048468 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0051704 GO:0051716 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0072384 GO:0090559 GO:0097190 GO:0097345 GO:0098573 GO:0098588 GO:0098805 GO:0099111 GO:1902108 GO:1902110 GO:1902686 GO:1905710
KEGG Pathways
Metabolic & Signaling
ko04137 ko04214 map04137 map04214
Pfam Domains
Protein Families
EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

707

Amino Acids

78.27

Weight (kDa)

5.83

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1533
AatII GACGTC 1 cut(s) 1006
Acc65I GGTACC 2 cut(s) 535, 1777
AccB1I GGYRCC 4 cut(s) 254, 535, 821, 1777
AccB7I CCANNNNNTGG 1 cut(s) 1861
AccII CGCG 2 cut(s) 22, 363
AccIII TCCGGA 1 cut(s) 682
AcoI YGGCCR 5 cut(s) 180, 214, 240, 1827, 2053
AcsI RAATTY 3 cut(s) 1244, 1614, 1637
AcuI CTGAAG 4 cut(s) 363, 441, 1163, 1641
AcvI CACGTG 1 cut(s) 1196
AcyI GRCGYC 1 cut(s) 1003
AfaI GTAC 4 cut(s) 25, 496, 537, 1779
AfiI CCNNNNNNNGG 6 cut(s) 333, 786, 1625, 1861, 1954, 1955
AflIII ACRYGT 2 cut(s) 20, 361
AgsI TTSAA 9 cut(s) 177, 430, 641, 760, 784, 868, 1205, 1835, 1909
AjnI CCWGG 6 cut(s) 852, 1366, 1579, 1712, 1854, 1953
AloI GAACNNNNNNTCC 2 cut(s) 1858, 1890
Alw21I GWGCWC 1 cut(s) 1947
Alw26I GTCTC 4 cut(s) 240, 1004, 1754, 1961
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 9 cut(s) 180, 214, 240, 727, 1182, 1305, 1514, 1827, 2053
ApaI GGGCCC 3 cut(s) 731, 1186, 1309
ApoI RAATTY 3 cut(s) 1244, 1614, 1637
AseI ATTAAT 1 cut(s) 1073
Asp700I GAANNNNTTC 3 cut(s) 136, 363, 848
Asp718I GGTACC 2 cut(s) 535, 1777
AspLEI GCGC 1 cut(s) 1892
AsuC2I CCSGG 2 cut(s) 259, 302
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 4 cut(s) 763, 1466, 1586, 1983
AxyI CCTNAGG 1 cut(s) 1028
BaeGI GKGCMC 4 cut(s) 731, 826, 1186, 1309
BaeI ACNNNNGTAYC 2 cut(s) 7, 40
BalI TGGCCA 3 cut(s) 182, 216, 2055
BanI GGYRCC 4 cut(s) 254, 535, 821, 1777
BanII GRGCYC 4 cut(s) 99, 731, 1186, 1309
BarI GAAGNNNNNNTAC 2 cut(s) 1025, 1057
BbrPI CACGTG 1 cut(s) 1196
Bbv12I GWGCWC 1 cut(s) 1947
BcgI CGANNNNNNTGC 4 cut(s) 611, 645, 1806, 1840
BciT130I CCWGG 6 cut(s) 854, 1368, 1581, 1714, 1856, 1955
BciVI GTATCC 2 cut(s) 397, 1034
BclI TGATCA 3 cut(s) 805, 1102, 1573
BcnI CCSGG 2 cut(s) 259, 302
BcoDI GTCTC 4 cut(s) 240, 1004, 1754, 1961
BfaI CTAG 8 cut(s) 44, 92, 524, 563, 1007, 1398, 1601, 1749
BfmI CTRYAG 1 cut(s) 2047
BfuI GTATCC 2 cut(s) 397, 1034
Bme1390I CCNGG 8 cut(s) 259, 302, 854, 1368, 1581, 1714, 1856, 1955
Bme18I GGWCC 4 cut(s) 763, 1466, 1586, 1983
BmeT110I CYCGRG 1 cut(s) 393
BmrFI CCNGG 8 cut(s) 259, 302, 854, 1368, 1581, 1714, 1856, 1955
BmsI GCATC 4 cut(s) 430, 657, 823, 1249
BpmI CTGGAG 1 cut(s) 1209
BpuMI CCSGG 2 cut(s) 259, 302
Bsa29I ATCGAT 2 cut(s) 487, 2007
BsaAI YACGTR 1 cut(s) 1196
BsaHI GRCGYC 1 cut(s) 1003
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 4 cut(s) 250, 1580, 1954, 2056
BsaWI WCCGGW 3 cut(s) 316, 682, 2106
BsaXI ACNNNNNCTCC 4 cut(s) 1858, 1888, 2035, 2065
Bsc4I CCNNNNNNNGG 6 cut(s) 333, 786, 1625, 1861, 1954, 1955
Bse1I ACTGG 1 cut(s) 1771
Bse21I CCTNAGG 1 cut(s) 1028
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 6 cut(s) 854, 1368, 1581, 1714, 1856, 1955
BseCI ATCGAT 2 cut(s) 487, 2007
BseDI CCNNGG 4 cut(s) 250, 1580, 1954, 2056
BseGI GGATG 5 cut(s) 718, 823, 1264, 1379, 1709
BseLI CCNNNNNNNGG 6 cut(s) 333, 786, 1625, 1861, 1954, 1955
BseMII CTCAG 8 cut(s) 488, 841, 1019, 1536, 1657, 1719, 1956, 1962
BseNI ACTGG 1 cut(s) 1771
BseRI GAGGAG 2 cut(s) 1562, 2108
BseSI GKGCMC 4 cut(s) 731, 826, 1186, 1309
BseYI CCCAGC 3 cut(s) 150, 1327, 1478
Bsh1236I CGCG 2 cut(s) 22, 363
BshFI GGCC 9 cut(s) 182, 216, 242, 729, 1184, 1307, 1516, 1829, 2055
BshNI GGYRCC 4 cut(s) 254, 535, 821, 1777
BshVI ATCGAT 2 cut(s) 487, 2007
BsiHKAI GWGCWC 1 cut(s) 1947
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 6 cut(s) 243, 258, 302, 317, 683, 2107
BslFI GGGAC 2 cut(s) 1014, 1599
BslI CCNNNNNNNGG 6 cut(s) 333, 786, 1625, 1861, 1954, 1955
BsmAI GTCTC 4 cut(s) 240, 1004, 1754, 1961
BsmFI GGGAC 2 cut(s) 1014, 1599
BsmI GAATGC 2 cut(s) 1817, 1959
BsnI GGCC 9 cut(s) 182, 216, 242, 729, 1184, 1307, 1516, 1829, 2055
Bso31I GGTCTC 1 cut(s) 240
BsoBI CYCGRG 1 cut(s) 393
Bsp120I GGGCCC 3 cut(s) 727, 1182, 1305
Bsp1286I GDGCHC 6 cut(s) 99, 731, 826, 1186, 1309, 1947
Bsp13I TCCGGA 1 cut(s) 682
Bsp19I CCATGG 2 cut(s) 250, 2056
BspANI GGCC 9 cut(s) 182, 216, 242, 729, 1184, 1307, 1516, 1829, 2055
BspCNI CTCAG 8 cut(s) 487, 840, 1020, 1537, 1656, 1720, 1957, 1963
BspDI ATCGAT 2 cut(s) 487, 2007
BspEI TCCGGA 1 cut(s) 682
BspFNI CGCG 2 cut(s) 22, 363
BspHI TCATGA 2 cut(s) 808, 1311
BspMAI CTGCAG 1 cut(s) 2051
BspT107I GGYRCC 4 cut(s) 254, 535, 821, 1777
BspTNI GGTCTC 1 cut(s) 240
BsrI ACTGG 1 cut(s) 1771
BssECI CCNNGG 4 cut(s) 250, 1580, 1954, 2056
BssNI GRCGYC 1 cut(s) 1003
BssT1I CCWWGG 2 cut(s) 250, 2056
Bst2UI CCWGG 6 cut(s) 854, 1368, 1581, 1714, 1856, 1955
Bst4CI ACNGT 5 cut(s) 627, 1024, 1169, 1302, 1697
Bst6I CTCTTC 2 cut(s) 417, 1712
BstACI GRCGYC 1 cut(s) 1003
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 1196
BstC8I GCNNGC 4 cut(s) 777, 1062, 1231, 1991
BstDSI CCRYGG 2 cut(s) 250, 2056
BstF5I GGATG 5 cut(s) 718, 823, 1264, 1379, 1709
BstFNI CGCG 2 cut(s) 22, 363
BstHHI GCGC 1 cut(s) 1892
BstMAI GTCTC 4 cut(s) 240, 1004, 1754, 1961
BstMWI GCNNNNNNNGC 8 cut(s) 53, 62, 68, 74, 319, 707, 1664, 2052
BstNI CCWGG 6 cut(s) 854, 1368, 1581, 1714, 1856, 1955
BstNSI RCATGY 2 cut(s) 465, 1671
BstSCI CCNGG 8 cut(s) 257, 300, 852, 1366, 1579, 1712, 1854, 1953
BstSFI CTRYAG 1 cut(s) 2047
BstSLI GKGCMC 4 cut(s) 731, 826, 1186, 1309
BstUI CGCG 2 cut(s) 22, 363
Bsu15I ATCGAT 2 cut(s) 487, 2007
Bsu36I CCTNAGG 1 cut(s) 1028
BsuI GTATCC 2 cut(s) 397, 1034
BsuRI GGCC 9 cut(s) 182, 216, 242, 729, 1184, 1307, 1516, 1829, 2055
BsuTUI ATCGAT 2 cut(s) 487, 2007
BtgI CCRYGG 2 cut(s) 250, 2056
BtsCI GGATG 5 cut(s) 718, 823, 1264, 1379, 1709
BtsI GCAGTG 1 cut(s) 2056
BtsIMutI CAGTG 5 cut(s) 835, 1307, 1542, 2044, 2056
Cac8I GCNNGC 4 cut(s) 777, 1062, 1231, 1991
CciI TCATGA 2 cut(s) 808, 1311
CfoI GCGC 1 cut(s) 1892
ClaI ATCGAT 2 cut(s) 487, 2007
Csp6I GTAC 4 cut(s) 24, 495, 536, 1778
CviAII CATG 9 cut(s) 251, 462, 809, 947, 980, 1312, 1668, 1754, 2057
CviQI GTAC 4 cut(s) 24, 495, 536, 1778
EaeI YGGCCR 5 cut(s) 180, 214, 240, 1827, 2053
Eam1104I CTCTTC 2 cut(s) 417, 1712
EarI CTCTTC 2 cut(s) 417, 1712
Eco130I CCWWGG 2 cut(s) 250, 2056
Eco24I GRGCYC 4 cut(s) 99, 731, 1186, 1309
Eco31I GGTCTC 1 cut(s) 240
Eco47I GGWCC 4 cut(s) 763, 1466, 1586, 1983
Eco57I CTGAAG 4 cut(s) 363, 441, 1163, 1641
Eco72I CACGTG 1 cut(s) 1196
Eco81I CCTNAGG 1 cut(s) 1028
Eco88I CYCGRG 1 cut(s) 393
EcoO109I RGGNCCY 5 cut(s) 1182, 1183, 1306, 1586, 1983
EcoRII CCWGG 6 cut(s) 852, 1366, 1579, 1712, 1854, 1953
EcoT14I CCWWGG 2 cut(s) 250, 2056
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 4 cut(s) 99, 731, 1186, 1309
ErhI CCWWGG 2 cut(s) 250, 2056
FaeI CATG 9 cut(s) 254, 465, 812, 950, 983, 1315, 1671, 1757, 2060
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 1136, 1168
FaqI GGGAC 2 cut(s) 1014, 1599
FatI CATG 9 cut(s) 250, 461, 808, 946, 979, 1311, 1667, 1753, 2056
FauI CCCGC 2 cut(s) 88, 1994
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 3 cut(s) 805, 1102, 1573
FokI GGATG 5 cut(s) 705, 830, 1271, 1366, 1696
FriOI GRGCYC 4 cut(s) 99, 731, 1186, 1309
FspBI CTAG 8 cut(s) 44, 92, 524, 563, 1007, 1398, 1601, 1749
GlaI GCGC 1 cut(s) 1891
GsaI CCCAGC 3 cut(s) 154, 1331, 1482
GsuI CTGGAG 1 cut(s) 1209
HaeIII GGCC 9 cut(s) 182, 216, 242, 729, 1184, 1307, 1516, 1829, 2055
HapII CCGG 6 cut(s) 243, 258, 302, 317, 683, 2107
HhaI GCGC 1 cut(s) 1892
Hin1I GRCGYC 1 cut(s) 1003
Hin1II CATG 9 cut(s) 254, 465, 812, 950, 983, 1315, 1671, 1757, 2060
Hin6I GCGC 1 cut(s) 1890
HinP1I GCGC 1 cut(s) 1890
HincII GTYRAC 1 cut(s) 521
HindII GTYRAC 1 cut(s) 521
HinfI GANTC 7 cut(s) 115, 679, 936, 1345, 1499, 1706, 2080
HpaII CCGG 6 cut(s) 243, 258, 302, 317, 683, 2107
Hpy166II GTNNAC 4 cut(s) 521, 1020, 1199, 1690
Hpy8I GTNNAC 4 cut(s) 521, 1020, 1199, 1690
HpyAV CCTTC 8 cut(s) 754, 894, 926, 991, 1138, 1468, 1506, 1974
HpyCH4III ACNGT 5 cut(s) 627, 1024, 1169, 1302, 1697
HpyCH4IV ACGT 3 cut(s) 1003, 1016, 1195
HpyF10VI GCNNNNNNNGC 8 cut(s) 53, 62, 68, 74, 319, 707, 1664, 2052
HpySE526I ACGT 3 cut(s) 1003, 1016, 1195
Hsp92I GRCGYC 1 cut(s) 1003
Hsp92II CATG 9 cut(s) 254, 465, 812, 950, 983, 1315, 1671, 1757, 2060
HspAI GCGC 1 cut(s) 1890
KflI GGGWCCC 1 cut(s) 1586
Kpn2I TCCGGA 1 cut(s) 682
KpnI GGTACC 2 cut(s) 539, 1781
Ksp22I TGATCA 3 cut(s) 805, 1102, 1573
LmnI GCTCC 6 cut(s) 877, 1114, 1228, 1549, 1879, 2043
LweI GCATC 4 cut(s) 430, 657, 823, 1249
MaeI CTAG 8 cut(s) 44, 92, 524, 563, 1007, 1398, 1601, 1749
MaeII ACGT 3 cut(s) 1003, 1016, 1195
MaeIII GTNAC 6 cut(s) 277, 609, 1296, 1393, 1670, 1930
MboII GAAGA 9 cut(s) 110, 141, 356, 434, 968, 1217, 1414, 1634, 1729
MfeI CAATTG 1 cut(s) 1131
MhlI GDGCHC 6 cut(s) 99, 731, 826, 1186, 1309, 1947
MlsI TGGCCA 3 cut(s) 182, 216, 2055
MluI ACGCGT 2 cut(s) 20, 361
MluNI TGGCCA 3 cut(s) 182, 216, 2055
MlyI GAGTC 3 cut(s) 945, 1715, 2089
MmeI TCCRAC 5 cut(s) 148, 746, 1213, 1562, 2042
Mox20I TGGCCA 3 cut(s) 182, 216, 2055
Mph1103I ATGCAT 1 cut(s) 463
MroI TCCGGA 1 cut(s) 682
MroXI GAANNNNTTC 3 cut(s) 136, 363, 848
MscI TGGCCA 3 cut(s) 182, 216, 2055
MseI TTAA 6 cut(s) 858, 903, 1073, 2013, 2111, 2119
MslI CAYNNNNRTG 1 cut(s) 1679
Msp20I TGGCCA 3 cut(s) 182, 216, 2055
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 6 cut(s) 243, 258, 302, 317, 683, 2107
MspR9I CCNGG 8 cut(s) 259, 302, 854, 1368, 1581, 1714, 1856, 1955
MunI CAATTG 1 cut(s) 1131
Mva1269I GAATGC 2 cut(s) 1817, 1959
MvaI CCWGG 6 cut(s) 854, 1368, 1581, 1714, 1856, 1955
MvnI CGCG 2 cut(s) 22, 363
MwoI GCNNNNNNNGC 8 cut(s) 53, 62, 68, 74, 319, 707, 1664, 2052
NciI CCSGG 2 cut(s) 259, 302
NcoI CCATGG 2 cut(s) 250, 2056
NdeI CATATG 1 cut(s) 12
NlaIII CATG 9 cut(s) 254, 465, 812, 950, 983, 1315, 1671, 1757, 2060
NmuCI GTSAC 5 cut(s) 277, 609, 1296, 1393, 1670
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 2 cut(s) 465, 1671
PaeR7I CTCGAG 1 cut(s) 393
PagI TCATGA 2 cut(s) 808, 1311
PctI GAATGC 2 cut(s) 1817, 1959
PdmI GAANNNNTTC 3 cut(s) 136, 363, 848
PfeI GAWTC 4 cut(s) 115, 679, 1345, 1499
PflMI CCANNNNNTGG 1 cut(s) 1861
PfoI TCCNGGA 1 cut(s) 1712
PleI GAGTC 3 cut(s) 944, 1714, 2088
PmaCI CACGTG 1 cut(s) 1196
PmlI CACGTG 1 cut(s) 1196
PpsI GAGTC 3 cut(s) 944, 1714, 2088
Ppu21I YACGTR 1 cut(s) 1196
PpuMI RGGWCCY 2 cut(s) 1586, 1983
PshBI ATTAAT 1 cut(s) 1073
PsiI TTATAA 1 cut(s) 1533
Psp5II RGGWCCY 2 cut(s) 1586, 1983
Psp6I CCWGG 6 cut(s) 852, 1366, 1579, 1712, 1854, 1953
PspCI CACGTG 1 cut(s) 1196
PspFI CCCAGC 3 cut(s) 150, 1327, 1478
PspGI CCWGG 6 cut(s) 852, 1366, 1579, 1712, 1854, 1953
PspOMI GGGCCC 3 cut(s) 727, 1182, 1305
PspPPI RGGWCCY 2 cut(s) 1586, 1983
PstI CTGCAG 1 cut(s) 2051
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 4 cut(s) 25, 496, 537, 1779
RsaNI GTAC 4 cut(s) 24, 495, 536, 1778
RseI CAYNNNNRTG 1 cut(s) 1679
SaqAI TTAA 6 cut(s) 858, 903, 1073, 2013, 2111, 2119
SchI GAGTC 3 cut(s) 945, 1715, 2089
ScrFI CCNGG 8 cut(s) 259, 302, 854, 1368, 1581, 1714, 1856, 1955
SduI GDGCHC 6 cut(s) 99, 731, 826, 1186, 1309, 1947
SfaNI GCATC 4 cut(s) 430, 657, 823, 1249
SfcI CTRYAG 1 cut(s) 2047
Sfr274I CTCGAG 1 cut(s) 393
SinI GGWCC 4 cut(s) 763, 1466, 1586, 1983
SlaI CTCGAG 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 1679
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
SspMI CTAG 8 cut(s) 44, 92, 524, 563, 1007, 1398, 1601, 1749
StyD4I CCNGG 8 cut(s) 257, 300, 852, 1366, 1579, 1712, 1854, 1953
StyI CCWWGG 2 cut(s) 250, 2056
TaaI ACNGT 5 cut(s) 627, 1024, 1169, 1302, 1697
TaiI ACGT 3 cut(s) 1006, 1019, 1198
TaqI TCGA 9 cut(s) 35, 113, 381, 394, 487, 597, 651, 738, 2007
TatI WGTACW 1 cut(s) 494
TauI GCSGC 4 cut(s) 68, 71, 1940, 2073
TfiI GAWTC 4 cut(s) 115, 679, 1345, 1499
Tru1I TTAA 6 cut(s) 858, 903, 1073, 2013, 2111, 2119
Tru9I TTAA 6 cut(s) 858, 903, 1073, 2013, 2111, 2119
TscAI CASTG 5 cut(s) 835, 1307, 1549, 2044, 2056
TseFI GTSAC 5 cut(s) 277, 609, 1296, 1393, 1670
Tsp45I GTSAC 5 cut(s) 277, 609, 1296, 1393, 1670
TspDTI ATGAA 8 cut(s) 17, 707, 784, 957, 968, 979, 1083, 1368
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 5 cut(s) 835, 1307, 1549, 2044, 2056
Van91I CCANNNNNTGG 1 cut(s) 1861
VpaK11BI GGWCC 4 cut(s) 763, 1466, 1586, 1983
VspI ATTAAT 1 cut(s) 1073
XapI RAATTY 3 cut(s) 1244, 1614, 1637
XbaI TCTAGA 1 cut(s) 1006
XceI RCATGY 2 cut(s) 465, 1671
XcmI CCANNNNNNNNNTGG 1 cut(s) 2063
XhoI CTCGAG 1 cut(s) 393
XmnI GAANNNNTTC 3 cut(s) 136, 363, 848
XspI CTAG 8 cut(s) 44, 92, 524, 563, 1007, 1398, 1601, 1749
ZraI GACGTC 1 cut(s) 1004
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.