AT5G36250

protein phosphatase 2C 74

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006070
Physical Location & Seq
Forward (+)
14282059 .. 14284684
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G36250.2

Sequence Viewer

Length: 1347 bp
ATGGGGTCCTGCTTATCATCATCTGGAGGTGGAGGAAGCAGGAGATCTCTCCATGGCTCTCCTCATGTTCCTGGTCCTGGGAGGAGGAAGAGGCCTCCCAAGAGGAGACCTGGGTCTTGTAGTTCTTCGTTTGATAACACCGAGGAGCCACTTCTTCATAGGATCCCTGGAAGGATGTTTTTGAATGGCTCTACAGACACCGTGTCTCTCTTCTCTCAACAGGGCAAGAAAGGACCTAATCAGGACGCTATGATTGTTTGGGAGAATTTTGGGTCTATGGAGGACACTGTGTTTTGTGGTGTTTTTGATGGTCATGGTCCGTATGGTCACATTGTTGCAAAGAGAGTGAGGGATTTGCTTCCACTCAAGTTAGGTTCTCATTTGGAGTCCTATGTGTCCCCTGAGGAGGTTCTTAAAGAGATTAGCCTTAACACTGATGATAGAAAGATCTCAGAAGACCTTGTACATATATCTGCCAATGGAGAATCACGAGTCTACAATAAGGATTATGTGAAGGATCAAGATATGATTCAAATGCTAATAGGCTCAATTGTTAAAGCCTACAGATTCATGGATAAGGAATTGAAGATGCAAGTTGATGTTGATTGTTTTTGCAGTGGAACAACTGCAGTTACCATGGTTAAGCAGGGTCAACATCTTGTTATCGGGAATATCGGGGATTCAAGAGCAGTATTGGGTGTGAGGAACAAAGACAATAAACTTGTTCCTTTTCAATTAACTGAAGATCTCAAACCAGATGTTCCAGCTGAAGCAGAAAGGATAAAGAGATGCAGAGGACGTATATTTGCTCTTCGAGATGAACCGGGAGTTGCCCGTCTGTGGCTTCCTAACCACAACTCACCTGGTCTAGCCATGGCACGTGCTTTTGGAGATTTTTGCCTCAAAGACTTCGGTCTAATTTCTGTACCTGACGTCTCCTACCGACGCCTCACTGAGAAGGATGAATTTGTTGTCTTGGCAACTGATGGGATTTGGGATGCATTGACGAATGAAGAGGTTGTAAAGATAGTAGCGAAAGCTCCAACGCGTTCCTCAGCAGGTCGAGCATTGGTGGAGGCTGCAGTGCGTAACTGGAGATGGAAGTTCCCGACTTCAAAAGTCGATGACTGTGCCGTAGTTTGCCTATTCCTTGACTCCGAACCAAACAGATTGTCAACTGCTTCTTTCTCAAAAGAAAAACACATCAACAATGGCGTTACTGAACCTGAACCAGACACAGCATCATCATCAACTCCAGACTCAGGAACCGGATCGCCTGAGCTCAATGGAGTCAACAGAATCGATACACTTGTGAATCTCCCAGTATATGTCCCAACCAAAGAGTAG

Protein Analysis

448

Amino Acids

49.31

Weight (kDa)

7.13

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 186 - 361 6e-27 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 936
Acc36I ACCTGC 1 cut(s) 1049
AccI GTMKAC 1 cut(s) 495
AccII CGCG 1 cut(s) 1048
AclWI GGATC 4 cut(s) 157, 170, 525, 1279
AcsI RAATTY 2 cut(s) 265, 965
AcuI CTGAAG 2 cut(s) 762, 789
AcvI CACGTG 1 cut(s) 881
AcyI GRCGYC 2 cut(s) 933, 946
AfaI GTAC 2 cut(s) 465, 927
AfiI CCNNNNNNNGG 4 cut(s) 77, 406, 840, 1262
AflIII ACRYGT 1 cut(s) 1046
AgsI TTSAA 6 cut(s) 184, 533, 586, 684, 734, 1116
AhdI GACNNNNNGTC 1 cut(s) 202
AjnI CCWGG 5 cut(s) 70, 76, 109, 166, 862
AluBI AGCT 3 cut(s) 767, 1040, 1282
AluI AGCT 3 cut(s) 767, 1040, 1282
Alw21I GWGCWC 1 cut(s) 1284
Alw26I GTCTC 3 cut(s) 100, 210, 940
AlwI GGATC 4 cut(s) 157, 170, 525, 1279
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 1 cut(s) 1079
ApoI RAATTY 2 cut(s) 265, 965
ArsI GACNNNNNNTTYG 2 cut(s) 1157, 1189
AspS9I GGNCC 4 cut(s) 6, 74, 233, 317
AsuC2I CCSGG 1 cut(s) 825
AsuHPI GGTGA 1 cut(s) 852
AvaII GGWCC 4 cut(s) 6, 74, 233, 317
AxyI CCTNAGG 1 cut(s) 402
BaeI ACNNNNGTAYC 2 cut(s) 1296, 1329
BamHI GGATCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 1284
BarI GAAGNNNNNNTAC 2 cut(s) 447, 479
BauI CACGAG 1 cut(s) 489
BbrPI CACGTG 1 cut(s) 881
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 1284
BbvCI CCTCAGC 1 cut(s) 1054
BbvI GCAGC 1 cut(s) 1066
BccI CCATC 3 cut(s) 302, 980, 1092
BceAI ACGGC 1 cut(s) 1118
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 5 cut(s) 72, 78, 111, 168, 864
BcnI CCSGG 1 cut(s) 825
BcoDI GTCTC 3 cut(s) 100, 210, 940
BfaI CTAG 1 cut(s) 869
BfmI CTRYAG 4 cut(s) 192, 562, 627, 1080
BfuAI ACCTGC 1 cut(s) 1049
BglII AGATCT 3 cut(s) 44, 447, 745
BisI GCNGC 1 cut(s) 1080
BlsI GCNGC 1 cut(s) 1081
Bme1390I CCNGG 6 cut(s) 72, 78, 111, 168, 825, 864
Bme18I GGWCC 4 cut(s) 6, 74, 233, 317
BmeRI GACNNNNNGTC 1 cut(s) 202
BmgT120I GGNCC 4 cut(s) 6, 74, 233, 317
BmiI GGNNCC 4 cut(s) 7, 147, 164, 1267
BmrFI CCNGG 6 cut(s) 72, 78, 111, 168, 825, 864
BmrI ACTGGG 1 cut(s) 1316
BmsI GCATC 4 cut(s) 579, 779, 988, 1250
BmuI ACTGGG 1 cut(s) 1316
BoxI GACNNNNGTC 2 cut(s) 112, 912
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 3 cut(s) 45, 1114, 1239
Bpu10I CCTNAGC 2 cut(s) 1054, 1278
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 825
Bsa29I ATCGAT 1 cut(s) 1302
BsaAI YACGTR 1 cut(s) 881
BsaHI GRCGYC 2 cut(s) 933, 946
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 7 cut(s) 52, 77, 110, 141, 166, 636, 873
BsaWI WCCGGW 1 cut(s) 1268
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 406, 840, 1262
Bse1I ACTGG 2 cut(s) 1097, 1322
Bse21I CCTNAGG 1 cut(s) 402
BseBI CCWGG 5 cut(s) 72, 78, 111, 168, 864
BseCI ATCGAT 1 cut(s) 1302
BseDI CCNNGG 7 cut(s) 52, 77, 110, 141, 166, 636, 873
BseGI GGATG 3 cut(s) 180, 967, 1003
BseLI CCNNNNNNNGG 4 cut(s) 77, 406, 840, 1262
BseMII CTCAG 6 cut(s) 393, 465, 945, 1068, 1269, 1275
BseNI ACTGG 2 cut(s) 1097, 1322
BseRI GAGGAG 5 cut(s) 51, 97, 118, 158, 419
BseXI GCAGC 1 cut(s) 1066
Bsh1236I CGCG 1 cut(s) 1048
BshFI GGCC 1 cut(s) 94
BshVI ATCGAT 1 cut(s) 1302
BsiHKAI GWGCWC 1 cut(s) 1284
BsiSI CCGG 2 cut(s) 824, 1269
BslFI GGGAC 2 cut(s) 382, 1316
BslI CCNNNNNNNGG 4 cut(s) 77, 406, 840, 1262
BsmAI GTCTC 3 cut(s) 100, 210, 940
BsmBI CGTCTC 1 cut(s) 940
BsmFI GGGAC 2 cut(s) 382, 1316
BsnI GGCC 1 cut(s) 94
Bso31I GGTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 1284
Bsp1407I TGTACA 1 cut(s) 463
Bsp143I GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
Bsp19I CCATGG 3 cut(s) 52, 636, 873
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 6 cut(s) 394, 464, 946, 1067, 1270, 1274
BspDI ATCGAT 1 cut(s) 1302
BspFNI CGCG 1 cut(s) 1048
BspLI GGNNCC 4 cut(s) 7, 147, 164, 1267
BspMAI CTGCAG 2 cut(s) 631, 1084
BspMI ACCTGC 1 cut(s) 1049
BspPI GGATC 4 cut(s) 157, 170, 525, 1279
BspQI GCTCTTC 1 cut(s) 816
BspTNI GGTCTC 1 cut(s) 100
BsrGI TGTACA 1 cut(s) 463
BsrI ACTGG 2 cut(s) 1097, 1322
BssECI CCNNGG 7 cut(s) 52, 77, 110, 141, 166, 636, 873
BssMI GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
BssNI GRCGYC 2 cut(s) 933, 946
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 3 cut(s) 52, 636, 873
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 5 cut(s) 72, 78, 111, 168, 864
Bst4CI ACNGT 3 cut(s) 202, 289, 1130
Bst6I CTCTTC 4 cut(s) 83, 215, 816, 1008
BstACI GRCGYC 2 cut(s) 933, 946
BstAUI TGTACA 1 cut(s) 463
BstBAI YACGTR 1 cut(s) 881
BstDEI CTNAG 6 cut(s) 402, 451, 954, 1054, 1261, 1278
BstDSI CCRYGG 3 cut(s) 52, 636, 873
BstF5I GGATG 3 cut(s) 180, 967, 1003
BstFNI CGCG 1 cut(s) 1048
BstKTI GATC 6 cut(s) 47, 165, 450, 520, 748, 1274
BstMAI GTCTC 3 cut(s) 100, 210, 940
BstMBI GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
BstMWI GCNNNNNNNGC 1 cut(s) 1064
BstNI CCWGG 5 cut(s) 72, 78, 111, 168, 864
BstPAI GACNNNNGTC 2 cut(s) 112, 912
BstSCI CCNGG 6 cut(s) 70, 76, 109, 166, 823, 862
BstSFI CTRYAG 4 cut(s) 192, 562, 627, 1080
BstUI CGCG 1 cut(s) 1048
BstV1I GCAGC 1 cut(s) 1066
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 4 cut(s) 44, 162, 447, 745
BstYI RGATCY 4 cut(s) 44, 162, 447, 745
Bsu15I ATCGAT 1 cut(s) 1302
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 1 cut(s) 94
BsuTUI ATCGAT 1 cut(s) 1302
BtgI CCRYGG 3 cut(s) 52, 636, 873
BtsCI GGATG 3 cut(s) 180, 967, 1003
BtsI GCAGTG 2 cut(s) 622, 1089
BtsIMutI CAGTG 5 cut(s) 285, 432, 622, 951, 1089
BveI ACCTGC 1 cut(s) 1049
Cfr13I GGNCC 4 cut(s) 6, 74, 233, 317
ClaI ATCGAT 1 cut(s) 1302
CseI GACGC 2 cut(s) 254, 954
CsiI ACCWGGT 1 cut(s) 862
Csp6I GTAC 2 cut(s) 464, 926
CviAII CATG 6 cut(s) 53, 65, 314, 571, 637, 874
CviQI GTAC 2 cut(s) 464, 926
DdeI CTNAG 6 cut(s) 402, 451, 954, 1054, 1261, 1278
DpnI GATC 6 cut(s) 46, 164, 449, 519, 747, 1273
DpnII GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
DriI GACNNNNNGTC 1 cut(s) 202
Eam1104I CTCTTC 4 cut(s) 83, 215, 816, 1008
Eam1105I GACNNNNNGTC 1 cut(s) 202
EarI CTCTTC 4 cut(s) 83, 215, 816, 1008
Ecl136II GAGCTC 1 cut(s) 1282
Eco130I CCWWGG 3 cut(s) 52, 636, 873
Eco147I AGGCCT 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 1284
Eco31I GGTCTC 1 cut(s) 100
Eco47I GGWCC 4 cut(s) 6, 74, 233, 317
Eco53kI GAGCTC 1 cut(s) 1282
Eco57I CTGAAG 2 cut(s) 762, 789
Eco72I CACGTG 1 cut(s) 881
Eco81I CCTNAGG 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 1282
EcoO109I RGGNCCY 2 cut(s) 6, 233
EcoRII CCWGG 5 cut(s) 70, 76, 109, 166, 862
EcoT14I CCWWGG 3 cut(s) 52, 636, 873
EcoT22I ATGCAT 1 cut(s) 1003
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 3 cut(s) 52, 636, 873
Esp3I CGTCTC 1 cut(s) 940
FaeI CATG 6 cut(s) 56, 68, 317, 574, 640, 877
FaqI GGGAC 2 cut(s) 382, 1316
FatI CATG 6 cut(s) 52, 64, 313, 570, 636, 873
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 1080
FokI GGATG 3 cut(s) 187, 974, 1010
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 1 cut(s) 1080
FspBI CTAG 1 cut(s) 869
GluI GCNGC 1 cut(s) 1080
GsuI CTGGAG 3 cut(s) 45, 1114, 1239
HaeIII GGCC 1 cut(s) 94
HapII CCGG 2 cut(s) 824, 1269
HgaI GACGC 2 cut(s) 254, 954
Hin1I GRCGYC 2 cut(s) 933, 946
Hin1II CATG 6 cut(s) 56, 68, 317, 574, 640, 877
HincII GTYRAC 3 cut(s) 653, 1176, 1294
HindII GTYRAC 3 cut(s) 653, 1176, 1294
HpaII CCGG 2 cut(s) 824, 1269
HphI GGTGA 1 cut(s) 852
Hpy166II GTNNAC 4 cut(s) 496, 653, 1176, 1294
Hpy188I TCNGA 2 cut(s) 454, 1159
Hpy8I GTNNAC 4 cut(s) 496, 653, 1176, 1294
Hpy99I CGWCG 1 cut(s) 948
HpyAV CCTTC 3 cut(s) 165, 508, 952
HpyCH4III ACNGT 3 cut(s) 202, 289, 1130
HpyCH4IV ACGT 3 cut(s) 799, 880, 933
HpyCH4V TGCA 7 cut(s) 338, 592, 615, 629, 792, 1001, 1082
HpyF10VI GCNNNNNNNGC 1 cut(s) 1064
HpyF3I CTNAG 6 cut(s) 402, 451, 954, 1054, 1261, 1278
HpySE526I ACGT 3 cut(s) 799, 880, 933
Hsp92I GRCGYC 2 cut(s) 933, 946
Hsp92II CATG 6 cut(s) 56, 68, 317, 574, 640, 877
Kzo9I GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
LguI GCTCTTC 1 cut(s) 816
LmnI GCTCC 2 cut(s) 145, 1045
Lsp1109I GCAGC 1 cut(s) 1066
LweI GCATC 4 cut(s) 579, 779, 988, 1250
MabI ACCWGGT 1 cut(s) 862
MaeI CTAG 1 cut(s) 869
MaeII ACGT 3 cut(s) 799, 880, 933
MaeIII GTNAC 4 cut(s) 326, 631, 1088, 1216
MalI GATC 6 cut(s) 46, 164, 449, 519, 747, 1273
MboI GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
MboII GAAGA 9 cut(s) 100, 117, 146, 202, 467, 598, 755, 803, 1025
MfeI CAATTG 1 cut(s) 549
MflI RGATCY 4 cut(s) 44, 162, 447, 745
MhlI GDGCHC 1 cut(s) 1284
MluCI AATT 6 cut(s) 265, 549, 581, 734, 918, 965
MluI ACGCGT 1 cut(s) 1046
MlyI GAGTC 5 cut(s) 395, 501, 1148, 1253, 1299
MmeI TCCRAC 1 cut(s) 1067
Mph1103I ATGCAT 1 cut(s) 1003
MseI TTAA 5 cut(s) 414, 429, 555, 642, 737
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 2 cut(s) 824, 1269
MspR9I CCNGG 6 cut(s) 72, 78, 111, 168, 825, 864
MunI CAATTG 1 cut(s) 549
MvaI CCWGG 5 cut(s) 72, 78, 111, 168, 864
MvnI CGCG 1 cut(s) 1048
MwoI GCNNNNNNNGC 1 cut(s) 1064
NciI CCSGG 1 cut(s) 825
NcoI CCATGG 3 cut(s) 52, 636, 873
NdeII GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
NlaIII CATG 6 cut(s) 56, 68, 317, 574, 640, 877
NlaIV GGNNCC 4 cut(s) 7, 147, 164, 1267
NmuCI GTSAC 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 816
PfeI GAWTC 6 cut(s) 485, 529, 567, 680, 1299, 1315
PkrI GCNGC 1 cut(s) 1081
PleI GAGTC 5 cut(s) 394, 500, 1148, 1253, 1298
PmaCI CACGTG 1 cut(s) 881
PmlI CACGTG 1 cut(s) 881
PpsI GAGTC 5 cut(s) 394, 500, 1148, 1253, 1298
Ppu21I YACGTR 1 cut(s) 881
PpuMI RGGWCCY 2 cut(s) 6, 233
PshAI GACNNNNGTC 2 cut(s) 112, 912
Psp124BI GAGCTC 1 cut(s) 1284
Psp5II RGGWCCY 2 cut(s) 6, 233
Psp6I CCWGG 5 cut(s) 70, 76, 109, 166, 862
PspCI CACGTG 1 cut(s) 881
PspGI CCWGG 5 cut(s) 70, 76, 109, 166, 862
PspN4I GGNNCC 4 cut(s) 7, 147, 164, 1267
PspPI GGNCC 4 cut(s) 6, 74, 233, 317
PspPPI RGGWCCY 2 cut(s) 6, 233
PstI CTGCAG 2 cut(s) 631, 1084
PsuI RGATCY 4 cut(s) 44, 162, 447, 745
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 2 cut(s) 465, 927
RsaNI GTAC 2 cut(s) 464, 926
SacI GAGCTC 1 cut(s) 1284
SapI GCTCTTC 1 cut(s) 816
SaqAI TTAA 5 cut(s) 414, 429, 555, 642, 737
SatI GCNGC 1 cut(s) 1080
Sau3AI GATC 6 cut(s) 44, 162, 447, 517, 745, 1271
Sau96I GGNCC 4 cut(s) 6, 74, 233, 317
SchI GAGTC 5 cut(s) 395, 501, 1148, 1253, 1299
ScrFI CCNGG 6 cut(s) 72, 78, 111, 168, 825, 864
SduI GDGCHC 1 cut(s) 1284
SexAI ACCWGGT 1 cut(s) 862
SfaNI GCATC 4 cut(s) 579, 779, 988, 1250
SfcI CTRYAG 4 cut(s) 192, 562, 627, 1080
SinI GGWCC 4 cut(s) 6, 74, 233, 317
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 6 cut(s) 265, 549, 581, 734, 918, 965
SseBI AGGCCT 1 cut(s) 94
SspMI CTAG 1 cut(s) 869
SstI GAGCTC 1 cut(s) 1284
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 6 cut(s) 70, 76, 109, 166, 823, 862
StyI CCWWGG 3 cut(s) 52, 636, 873
TaaI ACNGT 3 cut(s) 202, 289, 1130
TaiI ACGT 3 cut(s) 802, 883, 936
TaqI TCGA 4 cut(s) 814, 1063, 1122, 1302
TaqII GACCGA 1 cut(s) 902
TasI AATT 6 cut(s) 265, 549, 581, 734, 918, 965
TatI WGTACW 1 cut(s) 463
TfiI GAWTC 6 cut(s) 485, 529, 567, 680, 1299, 1315
Tru1I TTAA 5 cut(s) 414, 429, 555, 642, 737
Tru9I TTAA 5 cut(s) 414, 429, 555, 642, 737
TscAI CASTG 5 cut(s) 292, 439, 622, 958, 1089
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 1 cut(s) 1079
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 5 cut(s) 146, 559, 834, 978, 1026
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 5 cut(s) 292, 439, 622, 958, 1089
VpaK11BI GGWCC 4 cut(s) 6, 74, 233, 317
XapI RAATTY 2 cut(s) 265, 965
XcmI CCANNNNNNNNNTGG 1 cut(s) 860
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 1 cut(s) 869
ZraI GACGTC 1 cut(s) 934
Zsp2I ATGCAT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.