Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006070

Physical Location & Seq

Genomic Coordinates Forward (+)

24356000 .. 24358836

Gene Structure 2837 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g23920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGGTTCCTGCTTGTCTGTAGAAAGCAGGAGCCCTCTGCCCGATACGCCGTCGTCTCCCTCCTATGAAGTCCGGCGGTGGAAGAGCTCTAAGAAGAGAATGGGGTCGAGAAATTCGTCCTTCGATTACCGCCGGGAAGAACAGCTGCACAGAATTCCCGGGAGAATGTTCCTCAATGGGTCCAGTGAAGCTGCATCTTTGTTTACGCAGCAGGGCAAGAAAGGAACCAATCAGGATGCCATGATTGTTTGGGAGAATTTCTGTTCGAGAACAGACACAGTTTTCTGTGGTGTTTTCGATGGGCATGGTCCATTTGGTCATATGGTTGCTAAAAGAGTGAGGGACTCTCTTCCTCTGAAATTGAGTGCAAACTGGGAAGTTCTCAAAGAGATCAGCCTCAACACTGCAGGAAGCTTGAATTCAGATGATACTGCTTTTGTATCTGTTGATGAAGATCTCAGGCCCTCCGTGGATGTTGACGAAACAGAAAAAAACCCTGAGATCCTTCAGACACTGAGAGAGTCATTTCTGAAGGCTTTTAAAGTTATGGACAGGGAGCTGCGAACAACACCAAGTATCGATTGCTTTTGTAGTGGGACAACAGCAGTAACTTTGATTAAACAGGGTCGGGATTTATTCATTGGAAATGTTGGGGACTCCAGAGCCGTTCTGTGCACGAGAGATAGAGATGACACTCTAAGTGCAATTCAGTTGACTGTGGATCTCAAACCAAGTCTTCCAGCGGAAGCAGAGAGAATACGGAAGTGTAGAGGGCGCGTTTTTGCTCTTCAGGATGAACCTGAGGTTGCTCGAGTCTGGCTGCCGAACAATGACTCTCCTGGTCTTGCCATGGCACGTGCTTTTGGAGATTTCTGCCTCAAGGATTTTGGCCTCATCTCTGTGCCAGATGTATCCTATCGGCGTCTCACAGAGAAGGATGAATTTATAGTCCTGGCGACAGATGGGATTTGGGATGTTCTCTCTAACAAAGAAGTGGTAGATGTTATAGCAACAGCCCCTGCACGTCCTTCTGCAGCTCAAGCACTGGTAGAGACAGCAGTTAGAGTGTGGAGACAGAAGTACCCAACTTCCAAAGTAGATGACTGTGCTGTAGTTTGTCTCTTCCTTGACTTAGACTCAAACATAGTATCAACTGCTGCTAATAACGGGTCAAATGAGAAGGAGCAGCCTACTACGGCAGATGAGGTAAATGCTGAAGAAGATCTCTCGGAGACAGCTTCTTTGGTCCGCGCGGGGACTGTCCGAACTGCTGAAGAGATCCTTGCAGACAAGGAAGAAGACGAAGAGGACGAAGAAGAAGATGAAGAAGACGAAGAAGAAGGAGGAGGAAGAAAGGAGAATGCTGCAGAGGAAATGAATTCGGAACCAGGTGTAGAGTGGTCAGCTCTGGAAGGAGTTTCTCGGGTGAACACATTATTGAATCTGCCCAGATTTACGCCCACAAAAGAAGACGAGAAGATGTGGAAGAAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

499

Amino Acids

55.38

Weight (kDa)

4.69

Isoelectric Point (pI)

47.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	73 - 354	1.6e-40	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006070)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02750 AT3G02750 AT3G02750 AT5G36250 AT5G36250 AT5G36250
fragaria_vesca	FvH4_6g00430 FvH4_6g00430 FvH4_6g00430 FvH4_6g00430 FvH4_6g00430
malus_domestica	MD04G1245600.v1.1 MD13G1061100.v1.1
prunus_persica	Prupe.6G362100_v2.0.a1
pyrus_communis	pycom04g21650 pycom12g23920
rosa_chinensis	RchiOBHm_Chr3g0447761
rosa_laevigata	RLG00000025973
rosa_multiflora	Rmu_ssc0000391.1_g000017
rosa_roxburghii	Rroxscaffold_6G00425960
rosa_rugosa	Rorug02G0605400 Rorug02G0605500
rosa_samantha	Rh3AG005100 Rh3BG004700 Rh3CG005100 Rh3DG005000
rosa_wichuraiana	Rw3G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	777, 1251, 1253
AciI	CCGC	5 cut(s)	76, 130, 743, 1249, 1253
AclWI	GGATC	3 cut(s)	496, 729, 1273
AcsI	RAATTY	6 cut(s)	112, 153, 256, 418, 941, 1378
AcuI	CTGAAG	5 cut(s)	491, 551, 773, 1236, 1293
AcvI	CACGTG	1 cut(s)	857
AcyI	GRCGYC	1 cut(s)	922
AfaI	GTAC	1 cut(s)	1082
AgsI	TTSAA	2 cut(s)	418, 1441
AjiI	CACGTC	1 cut(s)	1025
AjnI	CCWGG	3 cut(s)	838, 951, 1387
AluBI	AGCT	8 cut(s)	87, 145, 191, 414, 559, 1037, 1238, 1406
AluI	AGCT	8 cut(s)	87, 145, 191, 414, 559, 1037, 1238, 1406
Alw21I	GWGCWC	2 cut(s)	89, 677
Alw26I	GTCTC	6 cut(s)	60, 929, 1046, 1066, 1124, 1226
Alw44I	GTGCAC	1 cut(s)	673
AlwI	GGATC	3 cut(s)	496, 729, 1273
AlwNI	CAGNNNCTG	2 cut(s)	514, 1019
Ama87I	CYCGRG	3 cut(s)	158, 810, 1422
AoxI	GGCC	2 cut(s)	461, 889
ApaLI	GTGCAC	1 cut(s)	673
ApeKI	GCWGC	9 cut(s)	145, 191, 208, 559, 820, 1034, 1157, 1186, 1364
ApoI	RAATTY	6 cut(s)	112, 153, 256, 418, 941, 1378
ArsI	GACNNNNNNTTYG	2 cut(s)	1225, 1257
AspLEI	GCGC	2 cut(s)	777, 1253
AspS9I	GGNCC	4 cut(s)	180, 308, 462, 1246
AsuC2I	CCSGG	3 cut(s)	134, 159, 160
AsuHPI	GGTGA	1 cut(s)	1438
AvaI	CYCGRG	3 cut(s)	158, 810, 1422
AvaII	GGWCC	3 cut(s)	180, 308, 1246
AxyI	CCTNAGG	1 cut(s)	801
BaeGI	GKGCMC	1 cut(s)	677
BaeI	ACNNNNGTAYC	2 cut(s)	1064, 1097
BanII	GRGCYC	2 cut(s)	35, 89
BauI	CACGAG	1 cut(s)	676
BbrPI	CACGTG	1 cut(s)	857
BbsI	GAAGAC	4 cut(s)	728, 1305, 1335, 1476
Bbv12I	GWGCWC	2 cut(s)	89, 677
BbvI	GCAGC	9 cut(s)	132, 178, 220, 546, 807, 1046, 1144, 1198, 1351
BccI	CCATC	2 cut(s)	293, 956
BceAI	ACGGC	3 cut(s)	34, 650, 1212
BciT130I	CCWGG	3 cut(s)	840, 953, 1389
BciVI	GTATCC	1 cut(s)	922
BcnI	CCSGG	3 cut(s)	134, 159, 160
BcoDI	GTCTC	6 cut(s)	60, 929, 1046, 1066, 1124, 1226
BfmI	CTRYAG	5 cut(s)	18, 405, 1032, 1110, 1365
BfuI	GTATCC	1 cut(s)	922
BglII	AGATCT	2 cut(s)	454, 1222
BisI	GCNGC	9 cut(s)	146, 192, 209, 560, 821, 1035, 1158, 1187, 1365
BlsI	GCNGC	9 cut(s)	147, 193, 210, 561, 822, 1036, 1159, 1188, 1366
Bme1390I	CCNGG	6 cut(s)	134, 159, 160, 840, 953, 1389
Bme18I	GGWCC	3 cut(s)	180, 308, 1246
BmeT110I	CYCGRG	3 cut(s)	158, 810, 1422
BmgBI	CACGTC	1 cut(s)	1025
BmgT120I	GGNCC	4 cut(s)	180, 308, 462, 1246
BmiI	GGNNCC	5 cut(s)	7, 32, 181, 226, 1386
BmrFI	CCNGG	6 cut(s)	134, 159, 160, 840, 953, 1389
BmrI	ACTGGG	1 cut(s)	382
BmsI	GCATC	2 cut(s)	203, 226
BmuI	ACTGGG	1 cut(s)	382
BpiI	GAAGAC	4 cut(s)	728, 1305, 1335, 1476
BplI	GAGNNNNNCTC	2 cut(s)	331, 363
BpmI	CTGGAG	1 cut(s)	643
BpuEI	CTTGAG	2 cut(s)	863, 1023
BpuMI	CCSGG	3 cut(s)	134, 159, 160
Bsa29I	ATCGAT	1 cut(s)	579
BsaAI	YACGTR	1 cut(s)	857
BsaBI	GATNNNNATC	1 cut(s)	453
BsaHI	GRCGYC	1 cut(s)	922
BsaJI	CCNNGG	3 cut(s)	158, 468, 849
Bse1I	ACTGG	3 cut(s)	183, 377, 1050
Bse21I	CCTNAGG	1 cut(s)	801
Bse8I	GATNNNNATC	1 cut(s)	453
BseBI	CCWGG	3 cut(s)	840, 953, 1389
BseCI	ATCGAT	1 cut(s)	579
BseDI	CCNNGG	3 cut(s)	158, 468, 849
BseGI	GGATG	5 cut(s)	241, 478, 799, 943, 979
BseJI	GATNNNNATC	1 cut(s)	453
BseMII	CTCAG	4 cut(s)	472, 489, 506, 792
BseNI	ACTGG	3 cut(s)	183, 377, 1050
BseRI	GAGGAG	1 cut(s)	1359
BseSI	GKGCMC	1 cut(s)	677
BseXI	GCAGC	9 cut(s)	132, 178, 220, 546, 807, 1046, 1144, 1198, 1351
BsgI	GTGCAG	2 cut(s)	131, 1005
Bsh1236I	CGCG	3 cut(s)	777, 1251, 1253
BshFI	GGCC	2 cut(s)	463, 891
BshVI	ATCGAT	1 cut(s)	579
BsiHKAI	GWGCWC	2 cut(s)	89, 677
BsiHKCI	CYCGRG	3 cut(s)	158, 810, 1422
BsiSI	CCGG	3 cut(s)	73, 133, 159
BslFI	GGGAC	4 cut(s)	356, 610, 668, 1270
BsmAI	GTCTC	6 cut(s)	60, 929, 1046, 1066, 1124, 1226
BsmBI	CGTCTC	2 cut(s)	60, 929
BsmFI	GGGAC	4 cut(s)	356, 610, 668, 1270
BsmI	GAATGC	1 cut(s)	1366
BsnI	GGCC	2 cut(s)	463, 891
BsoBI	CYCGRG	3 cut(s)	158, 810, 1422
Bsp1286I	GDGCHC	3 cut(s)	35, 89, 677
Bsp143I	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
Bsp19I	CCATGG	1 cut(s)	849
BspACI	CCGC	5 cut(s)	76, 130, 743, 1249, 1253
BspANI	GGCC	2 cut(s)	463, 891
BspCNI	CTCAG	4 cut(s)	471, 490, 507, 793
BspDI	ATCGAT	1 cut(s)	579
BspFNI	CGCG	3 cut(s)	777, 1251, 1253
BspLI	GGNNCC	5 cut(s)	7, 32, 181, 226, 1386
BspMAI	CTGCAG	3 cut(s)	409, 1036, 1369
BspPI	GGATC	3 cut(s)	496, 729, 1273
BspQI	GCTCTTC	2 cut(s)	77, 792
BsrI	ACTGG	3 cut(s)	183, 377, 1050
BssECI	CCNNGG	3 cut(s)	158, 468, 849
BssMI	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
BssNI	GRCGYC	1 cut(s)	922
BssSI	CACGAG	1 cut(s)	676
BssT1I	CCWWGG	1 cut(s)	849
Bst2BI	CACGAG	1 cut(s)	676
Bst2UI	CCWGG	3 cut(s)	840, 953, 1389
Bst4CI	ACNGT	4 cut(s)	280, 718, 1106, 1261
Bst6I	CTCTTC	7 cut(s)	77, 89, 354, 792, 1127, 1269, 1299
BstACI	GRCGYC	1 cut(s)	922
BstBAI	YACGTR	1 cut(s)	857
BstDEI	CTNAG	7 cut(s)	90, 458, 498, 515, 698, 801, 1132
BstDSI	CCRYGG	2 cut(s)	468, 849
BstF5I	GGATG	5 cut(s)	241, 478, 799, 943, 979
BstFNI	CGCG	3 cut(s)	777, 1251, 1253
BstHHI	GCGC	2 cut(s)	777, 1253
BstKTI	GATC	6 cut(s)	393, 457, 504, 724, 1225, 1281
BstMAI	GTCTC	6 cut(s)	60, 929, 1046, 1066, 1124, 1226
BstMBI	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
BstMWI	GCNNNNNNNGC	2 cut(s)	46, 1040
BstNI	CCWGG	3 cut(s)	840, 953, 1389
BstSCI	CCNGG	6 cut(s)	132, 157, 158, 838, 951, 1387
BstSFI	CTRYAG	5 cut(s)	18, 405, 1032, 1110, 1365
BstSLI	GKGCMC	1 cut(s)	677
BstUI	CGCG	3 cut(s)	777, 1251, 1253
BstV1I	GCAGC	9 cut(s)	132, 178, 220, 546, 807, 1046, 1144, 1198, 1351
BstV2I	GAAGAC	4 cut(s)	728, 1305, 1335, 1476
BstX2I	RGATCY	5 cut(s)	454, 501, 721, 1222, 1278
BstYI	RGATCY	5 cut(s)	454, 501, 721, 1222, 1278
Bsu15I	ATCGAT	1 cut(s)	579
Bsu36I	CCTNAGG	1 cut(s)	801
BsuI	GTATCC	1 cut(s)	922
BsuRI	GGCC	2 cut(s)	463, 891
BsuTUI	ATCGAT	1 cut(s)	579
BtgI	CCRYGG	2 cut(s)	468, 849
BtrI	CACGTC	1 cut(s)	1025
BtsCI	GGATG	5 cut(s)	241, 478, 799, 943, 979
BtsI	GCAGTG	1 cut(s)	402
BtsIMutI	CAGTG	4 cut(s)	190, 402, 512, 1043
CaiI	CAGNNNCTG	2 cut(s)	514, 1019
CfoI	GCGC	2 cut(s)	777, 1253
Cfr13I	GGNCC	4 cut(s)	180, 308, 462, 1246
Cfr9I	CCCGGG	1 cut(s)	158
ClaI	ATCGAT	1 cut(s)	579
CseI	GACGC	1 cut(s)	911
CsiI	ACCWGGT	1 cut(s)	1387
Csp6I	GTAC	1 cut(s)	1081
CviAII	CATG	3 cut(s)	241, 305, 850
CviQI	GTAC	1 cut(s)	1081
DdeI	CTNAG	7 cut(s)	90, 458, 498, 515, 698, 801, 1132
DpnI	GATC	6 cut(s)	392, 456, 503, 723, 1224, 1280
DpnII	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
DraI	TTTAAA	1 cut(s)	541
Eam1104I	CTCTTC	7 cut(s)	77, 89, 354, 792, 1127, 1269, 1299
EarI	CTCTTC	7 cut(s)	77, 89, 354, 792, 1127, 1269, 1299
Ecl136II	GAGCTC	1 cut(s)	87
Eco130I	CCWWGG	1 cut(s)	849
Eco24I	GRGCYC	2 cut(s)	35, 89
Eco47I	GGWCC	3 cut(s)	180, 308, 1246
Eco53kI	GAGCTC	1 cut(s)	87
Eco57I	CTGAAG	5 cut(s)	491, 551, 773, 1236, 1293
Eco72I	CACGTG	1 cut(s)	857
Eco81I	CCTNAGG	1 cut(s)	801
Eco88I	CYCGRG	3 cut(s)	158, 810, 1422
EcoICRI	GAGCTC	1 cut(s)	87
EcoO109I	RGGNCCY	1 cut(s)	462
EcoRI	GAATTC	3 cut(s)	153, 418, 1378
EcoRII	CCWGG	3 cut(s)	838, 951, 1387
EcoT14I	CCWWGG	1 cut(s)	849
EcoT38I	GRGCYC	2 cut(s)	35, 89
ErhI	CCWWGG	1 cut(s)	849
Esp3I	CGTCTC	2 cut(s)	60, 929
FaeI	CATG	3 cut(s)	244, 308, 853
FalI	AAGNNNNNCTT	2 cut(s)	1266, 1298
FaqI	GGGAC	4 cut(s)	356, 610, 668, 1270
FatI	CATG	3 cut(s)	240, 304, 849
FauI	CCCGC	1 cut(s)	1246
FauNDI	CATATG	1 cut(s)	321
Fnu4HI	GCNGC	9 cut(s)	146, 192, 209, 560, 821, 1035, 1158, 1187, 1365
FokI	GGATG	5 cut(s)	248, 485, 806, 950, 986
FriOI	GRGCYC	2 cut(s)	35, 89
Fsp4HI	GCNGC	9 cut(s)	146, 192, 209, 560, 821, 1035, 1158, 1187, 1365
GlaI	GCGC	2 cut(s)	776, 1252
GluI	GCNGC	9 cut(s)	146, 192, 209, 560, 821, 1035, 1158, 1187, 1365
GsuI	CTGGAG	1 cut(s)	643
HaeIII	GGCC	2 cut(s)	463, 891
HapII	CCGG	3 cut(s)	73, 133, 159
HgaI	GACGC	1 cut(s)	911
HhaI	GCGC	2 cut(s)	777, 1253
Hin1I	GRCGYC	1 cut(s)	922
Hin1II	CATG	3 cut(s)	244, 308, 853
Hin6I	GCGC	2 cut(s)	775, 1251
HinP1I	GCGC	2 cut(s)	775, 1251
HincII	GTYRAC	2 cut(s)	478, 714
HindII	GTYRAC	2 cut(s)	478, 714
HindIII	AAGCTT	1 cut(s)	412
HinfI	GANTC	7 cut(s)	344, 521, 656, 813, 833, 1136, 1441
HpaII	CCGG	3 cut(s)	73, 133, 159
HphI	GGTGA	1 cut(s)	1438
Hpy166II	GTNNAC	5 cut(s)	204, 478, 675, 714, 1429
Hpy188I	TCNGA	7 cut(s)	357, 424, 510, 531, 1231, 1265, 1384
Hpy188III	TCNNGA	7 cut(s)	108, 233, 267, 629, 660, 791, 1409
Hpy8I	GTNNAC	5 cut(s)	204, 478, 675, 714, 1429
Hpy99I	CGWCG	1 cut(s)	55
HpyAV	CCTTC	9 cut(s)	130, 515, 526, 928, 1038, 1174, 1334, 1406, 1483
HpyCH4III	ACNGT	4 cut(s)	280, 718, 1106, 1261
HpyCH4IV	ACGT	2 cut(s)	856, 1024
HpyF10VI	GCNNNNNNNGC	2 cut(s)	46, 1040
HpyF3I	CTNAG	7 cut(s)	90, 458, 498, 515, 698, 801, 1132
HpySE526I	ACGT	2 cut(s)	856, 1024
Hsp92I	GRCGYC	1 cut(s)	922
Hsp92II	CATG	3 cut(s)	244, 308, 853
HspAI	GCGC	2 cut(s)	775, 1251
Kzo9I	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
LguI	GCTCTTC	2 cut(s)	77, 792
LmnI	GCTCC	3 cut(s)	30, 556, 1183
Lsp1109I	GCAGC	9 cut(s)	132, 178, 220, 546, 807, 1046, 1144, 1198, 1351
LweI	GCATC	2 cut(s)	203, 226
MabI	ACCWGGT	1 cut(s)	1387
MaeII	ACGT	2 cut(s)	856, 1024
MaeIII	GTNAC	1 cut(s)	607
MalI	GATC	6 cut(s)	392, 456, 503, 723, 1224, 1280
MboI	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
MflI	RGATCY	5 cut(s)	454, 501, 721, 1222, 1278
MhlI	GDGCHC	3 cut(s)	35, 89, 677
MluCI	AATT	8 cut(s)	112, 153, 256, 359, 418, 705, 941, 1378
MlyI	GAGTC	6 cut(s)	338, 530, 650, 822, 827, 1130
MseI	TTAA	2 cut(s)	540, 618
MslI	CAYNNNNRTG	1 cut(s)	899
MspA1I	CMGCKG	2 cut(s)	145, 743
MspI	CCGG	3 cut(s)	73, 133, 159
MspR9I	CCNGG	6 cut(s)	134, 159, 160, 840, 953, 1389
Mva1269I	GAATGC	1 cut(s)	1366
MvaI	CCWGG	3 cut(s)	840, 953, 1389
MvnI	CGCG	3 cut(s)	777, 1251, 1253
MwoI	GCNNNNNNNGC	2 cut(s)	46, 1040
NciI	CCSGG	3 cut(s)	134, 159, 160
NcoI	CCATGG	1 cut(s)	849
NdeI	CATATG	1 cut(s)	321
NdeII	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
NlaIII	CATG	3 cut(s)	244, 308, 853
NlaIV	GGNNCC	5 cut(s)	7, 32, 181, 226, 1386
PaeR7I	CTCGAG	1 cut(s)	810
PciSI	GCTCTTC	2 cut(s)	77, 792
PcsI	WCGNNNNNNNCGW	2 cut(s)	113, 1308
PctI	GAATGC	1 cut(s)	1366
PfeI	GAWTC	1 cut(s)	1441
PkrI	GCNGC	9 cut(s)	147, 193, 210, 561, 822, 1036, 1159, 1188, 1366
PleI	GAGTC	6 cut(s)	338, 529, 650, 821, 827, 1130
PmaCI	CACGTG	1 cut(s)	857
PmlI	CACGTG	1 cut(s)	857
PpsI	GAGTC	6 cut(s)	338, 529, 650, 821, 827, 1130
Ppu21I	YACGTR	1 cut(s)	857
Psp124BI	GAGCTC	1 cut(s)	89
Psp6I	CCWGG	3 cut(s)	838, 951, 1387
PspCI	CACGTG	1 cut(s)	857
PspGI	CCWGG	3 cut(s)	838, 951, 1387
PspN4I	GGNNCC	5 cut(s)	7, 32, 181, 226, 1386
PspPI	GGNCC	4 cut(s)	180, 308, 462, 1246
PspXI	VCTCGAGB	1 cut(s)	810
PstI	CTGCAG	3 cut(s)	409, 1036, 1369
PstNI	CAGNNNCTG	2 cut(s)	514, 1019
PsuI	RGATCY	5 cut(s)	454, 501, 721, 1222, 1278
PvuII	CAGCTG	1 cut(s)	145
RsaI	GTAC	1 cut(s)	1082
RsaNI	GTAC	1 cut(s)	1081
RseI	CAYNNNNRTG	1 cut(s)	899
SacI	GAGCTC	1 cut(s)	89
SapI	GCTCTTC	2 cut(s)	77, 792
SaqAI	TTAA	2 cut(s)	540, 618
SatI	GCNGC	9 cut(s)	146, 192, 209, 560, 821, 1035, 1158, 1187, 1365
Sau3AI	GATC	6 cut(s)	390, 454, 501, 721, 1222, 1278
Sau96I	GGNCC	4 cut(s)	180, 308, 462, 1246
SchI	GAGTC	6 cut(s)	338, 530, 650, 822, 827, 1130
ScrFI	CCNGG	6 cut(s)	134, 159, 160, 840, 953, 1389
SduI	GDGCHC	3 cut(s)	35, 89, 677
SexAI	ACCWGGT	1 cut(s)	1387
SfaNI	GCATC	2 cut(s)	203, 226
SfcI	CTRYAG	5 cut(s)	18, 405, 1032, 1110, 1365
Sfr274I	CTCGAG	1 cut(s)	810
SinI	GGWCC	3 cut(s)	180, 308, 1246
SlaI	CTCGAG	1 cut(s)	810
SmaI	CCCGGG	1 cut(s)	160
SmiMI	CAYNNNNRTG	1 cut(s)	899
SmlI	CTYRAG	3 cut(s)	810, 878, 1038
SmoI	CTYRAG	3 cut(s)	810, 878, 1038
Sse9I	AATT	8 cut(s)	112, 153, 256, 359, 418, 705, 941, 1378
SsiI	CCGC	5 cut(s)	76, 130, 743, 1249, 1253
SstI	GAGCTC	1 cut(s)	89
StyD4I	CCNGG	6 cut(s)	132, 157, 158, 838, 951, 1387
StyI	CCWWGG	1 cut(s)	849
TaaI	ACNGT	4 cut(s)	280, 718, 1106, 1261
TaiI	ACGT	2 cut(s)	859, 1027
TaqI	TCGA	6 cut(s)	107, 123, 266, 297, 579, 811
TasI	AATT	8 cut(s)	112, 153, 256, 359, 418, 705, 941, 1378
TfiI	GAWTC	1 cut(s)	1441
Tru1I	TTAA	2 cut(s)	540, 618
Tru9I	TTAA	2 cut(s)	540, 618
TscAI	CASTG	4 cut(s)	190, 409, 519, 1050
TseI	GCWGC	9 cut(s)	145, 191, 208, 559, 820, 1034, 1157, 1186, 1364
TspDTI	ATGAA	7 cut(s)	81, 465, 628, 810, 954, 1338, 1391
TspGWI	ACGGA	2 cut(s)	457, 775
TspMI	CCCGGG	1 cut(s)	158
TspRI	CASTG	4 cut(s)	190, 409, 519, 1050
VneI	GTGCAC	1 cut(s)	673
VpaK11BI	GGWCC	3 cut(s)	180, 308, 1246
XapI	RAATTY	6 cut(s)	112, 153, 256, 418, 941, 1378
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1395
XhoI	CTCGAG	1 cut(s)	810
XmaI	CCCGGG	1 cut(s)	158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.