FvH4_6g00430

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006070
Physical Location & Seq
Reverse (-)
284413 .. 289653
5241 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00430.t4

Sequence Viewer

Length: 1500 bp
ATGGGTTCCTGCTTGTCCGCCGAGAGCAGGAGCCCAATGGCCTCTCCTTCGCCGGCCTCTCCTGGCTGGGGTGGTGCCTCCCGCCGCAGGAAGACCTCTAAGAAGAAATCTACTTCCCGGAGTTCTTCTTTTGATTACCGCCGGGAAGACCCCGCCCTGCATCGGATTCCGGGCAGAGTCTTCCTCAATGGCTCTACTCAAGCTGCTTCTTTGTTTACTCAACAGGGCAAGAAAGGGACCAACCAAGATGCCATGATTGTCTGGGAGAATTTTTGTTCAAGAACAGACACAATTTTCTGCGGTGTTTTTGATGGTCATGGTCCATATGGTCATATGGTTGCAAAGAGAGTGAGGGATTCGCTTCCTCTCAAGTTGAGCTCCAATTGGGAAGTGAACATAAACAGTGAGGAAGTTCTCAAAGAGATCAGCCCTAATACTGCTGGAAGCTTGAATTCAGATGATGCGGCAATTGTGCCTGTTGATGAAGATTTCAGGGCCTCCATTGACAGTGAGGAATCAGAAAAGCACCCTGAGATCTTTCACACATTGAAAGAGTCATTCTTAAAAGCTTTCAGAGTTATGGACAGGGAACTACGAATACAACCAAGTATTGATTGCTTTTGTAGTGGGACAACAGCAGTAACTCTAGTTAAGCAGGGTCGGGATATAATTGTCGGAAATGTTGGGGATTCCAGGGCAGTTCTATGCACAAGAGATAAGGATGACACTCTGCTGGCAGTTCAGTTGACTGTGGATCTCAAACCAAATCTTCCAGCGGAAGCAGAAAGAATACGGAAGTGTAGAGGACGTGTTTTTGCTCTTCATGATGAACCAGAGGTTTGTCGGGTCTGGCTACCAAACAATGACTCCCCTGGTCTTGCCATGGCACGGGCCTTTGGAGATTTTTGCCTCAAGGATTTCGGTTTGATCTCAGTGCCTGATGTGTCTTATCGGCGTCTCACTGAGAAGGATGAATTTATAGTCTTGGCCACAGATGGGATTTGGGATGTTCTGTCTAACAAAGAAGTGGTAGATATAATAGCATCAGCCCCTGCACGCTCCTCTGCAGCTCGAGCACTGGTTGAGTCAGCAGTTCGGGCCTGGAGACAAAAATACCCAACTTCAAAAGTAGATGACTGTGCTGTGGTCTGCCTATACCTCGACTCAGACGGAAATAATTTATCAACTGCTGCTAATAATAACTTGAGCGAGCAGCATACTGGGGTGGATGATTTGAATGCTGGGAGTGAGAATGAAGAAGATATCTCTGGCCCAACTTCTTTTGTCCGCCCAGGTACTGTCCGAAATGGTGAAGAGATTCTTGAAGACAACGAAGATGTAGAGGAAGAGAGAAGCAAGGAGAATGCTACAATGAACTCGGAATTGGGTGTAGACTGGTCTGCTCTGGAAGGAGTTTCTCGTGTTAACACATTGTTGACTCTGCCAAGATTTGTACCTGGAAAAGAAGACATTAAGGCCCGGAACAAGATTGGAAGTTGA

Protein Analysis

500

Amino Acids

54.83

Weight (kDa)

5.21

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 180 - 365 1.4e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 74
AccI GTMKAC 1 cut(s) 1392
AciI CCGC 9 cut(s) 18, 82, 85, 139, 153, 300, 464, 776, 1288
AclWI GGATC 1 cut(s) 762
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 3 cut(s) 268, 451, 974
AcyI GRCGYC 1 cut(s) 955
AfaI GTAC 2 cut(s) 1297, 1455
AfiI CCNNNNNNNGG 6 cut(s) 27, 68, 87, 162, 888, 996
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 6 cut(s) 279, 451, 550, 1125, 1237, 1325
AjiI CACGTC 1 cut(s) 809
AjnI CCWGG 6 cut(s) 61, 692, 871, 1100, 1291, 1456
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
AluBI AGCT 5 cut(s) 203, 378, 447, 569, 1070
AluI AGCT 5 cut(s) 203, 378, 447, 569, 1070
Alw21I GWGCWC 2 cut(s) 380, 1078
Alw26I GTCTC 2 cut(s) 962, 1099
AlwI GGATC 1 cut(s) 762
AlwNI CAGNNNCTG 3 cut(s) 938, 1052, 1298
Ama87I CYCGRG 1 cut(s) 1071
AoxI GGCC 8 cut(s) 39, 54, 495, 891, 987, 1098, 1270, 1476
ApeKI GCWGC 4 cut(s) 203, 1067, 1190, 1213
ApoI RAATTY 3 cut(s) 268, 451, 974
Asp700I GAANNNNTTC 1 cut(s) 1317
AspS9I GGNCC 7 cut(s) 237, 320, 495, 891, 1098, 1271, 1477
AsuC2I CCSGG 4 cut(s) 118, 143, 171, 1480
AsuHPI GGTGA 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 1071
AvaII GGWCC 2 cut(s) 237, 320
BalI TGGCCA 1 cut(s) 989
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 2 cut(s) 35, 380
BauI CACGAG 1 cut(s) 1419
BbsI GAAGAC 5 cut(s) 98, 153, 172, 1332, 1473
Bbv12I GWGCWC 2 cut(s) 380, 1078
BbvI GCAGC 4 cut(s) 190, 1079, 1177, 1225
BccI CCATC 2 cut(s) 305, 989
BciT130I CCWGG 6 cut(s) 63, 694, 873, 1102, 1293, 1458
BcnI CCSGG 4 cut(s) 118, 143, 171, 1480
BcoDI GTCTC 2 cut(s) 962, 1099
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 1065
BglII AGATCT 1 cut(s) 534
BisI GCNGC 6 cut(s) 85, 204, 465, 1068, 1191, 1214
BlsI GCNGC 6 cut(s) 86, 205, 466, 1069, 1192, 1215
Bme18I GGWCC 2 cut(s) 237, 320
BmeT110I CYCGRG 1 cut(s) 1071
BmgBI CACGTC 1 cut(s) 809
BmgT120I GGNCC 7 cut(s) 237, 320, 495, 891, 1098, 1271, 1477
BmiI GGNNCC 4 cut(s) 7, 32, 76, 238
BmrI ACTGGG 1 cut(s) 1230
BmsI GCATC 4 cut(s) 169, 238, 451, 1052
BmuI ACTGGG 1 cut(s) 1230
BpiI GAAGAC 5 cut(s) 98, 153, 172, 1332, 1473
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 1 cut(s) 1123
BpuEI CTTGAG 4 cut(s) 183, 353, 896, 1225
BpuMI CCSGG 4 cut(s) 118, 143, 171, 1480
BsaHI GRCGYC 1 cut(s) 955
BsaJI CCNNGG 4 cut(s) 693, 871, 882, 1291
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 6 cut(s) 27, 68, 87, 162, 888, 996
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 3 cut(s) 1083, 1225, 1400
BseBI CCWGG 6 cut(s) 63, 694, 873, 1102, 1293, 1458
BseDI CCNNGG 4 cut(s) 693, 871, 882, 1291
BseGI GGATG 4 cut(s) 727, 976, 1012, 1234
BseLI CCNNNNNNNGG 6 cut(s) 27, 68, 87, 162, 888, 996
BseMII CTCAG 4 cut(s) 522, 945, 954, 1179
BseNI ACTGG 3 cut(s) 1083, 1225, 1400
BseRI GAGGAG 1 cut(s) 1051
BseXI GCAGC 4 cut(s) 190, 1079, 1177, 1225
BseYI CCCAGC 2 cut(s) 66, 1241
BsgI GTGCAG 1 cut(s) 1038
BshFI GGCC 8 cut(s) 41, 56, 497, 893, 989, 1100, 1272, 1478
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 380, 1078
BsiHKCI CYCGRG 1 cut(s) 1071
BsiSI CCGG 5 cut(s) 53, 118, 142, 170, 1480
BslFI GGGAC 2 cut(s) 250, 643
BslI CCNNNNNNNGG 6 cut(s) 27, 68, 87, 162, 888, 996
BsmAI GTCTC 2 cut(s) 962, 1099
BsmBI CGTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 250, 643
BsmI GAATGC 2 cut(s) 1243, 1369
BsnI GGCC 8 cut(s) 41, 56, 497, 893, 989, 1100, 1272, 1478
BsoBI CYCGRG 1 cut(s) 1071
Bsp1286I GDGCHC 3 cut(s) 35, 380, 1078
Bsp143I GATC 4 cut(s) 423, 534, 754, 927
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 9 cut(s) 18, 82, 85, 139, 153, 300, 464, 776, 1288
BspANI GGCC 8 cut(s) 41, 56, 497, 893, 989, 1100, 1272, 1478
BspCNI CTCAG 4 cut(s) 523, 944, 955, 1178
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 4 cut(s) 7, 32, 76, 238
BspMAI CTGCAG 1 cut(s) 1069
BspPI GGATC 1 cut(s) 762
BspQI GCTCTTC 1 cut(s) 825
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 3 cut(s) 1083, 1225, 1400
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 4 cut(s) 693, 871, 882, 1291
BssMI GATC 4 cut(s) 423, 534, 754, 927
BssNI GRCGYC 1 cut(s) 955
BssSI CACGAG 1 cut(s) 1419
BssT1I CCWWGG 1 cut(s) 882
Bst2BI CACGAG 1 cut(s) 1419
Bst2UI CCWGG 6 cut(s) 63, 694, 873, 1102, 1293, 1458
Bst4CI ACNGT 5 cut(s) 404, 509, 751, 1139, 1300
Bst6I CTCTTC 3 cut(s) 825, 1308, 1341
BstACI GRCGYC 1 cut(s) 955
BstC8I GCNNGC 4 cut(s) 54, 735, 1057, 1211
BstDEI CTNAG 5 cut(s) 99, 531, 931, 963, 1165
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 4 cut(s) 727, 976, 1012, 1234
BstKTI GATC 4 cut(s) 426, 537, 757, 930
BstMAI GTCTC 2 cut(s) 962, 1099
BstMBI GATC 4 cut(s) 423, 534, 754, 927
BstMWI GCNNNNNNNGC 2 cut(s) 1073, 1097
BstNI CCWGG 6 cut(s) 63, 694, 873, 1102, 1293, 1458
BstSFI CTRYAG 1 cut(s) 1065
BstV1I GCAGC 4 cut(s) 190, 1079, 1177, 1225
BstV2I GAAGAC 5 cut(s) 98, 153, 172, 1332, 1473
BstX2I RGATCY 2 cut(s) 534, 754
BstYI RGATCY 2 cut(s) 534, 754
BsuRI GGCC 8 cut(s) 41, 56, 497, 893, 989, 1100, 1272, 1478
BtgI CCRYGG 1 cut(s) 882
BtrI CACGTC 1 cut(s) 809
BtsCI GGATG 4 cut(s) 727, 976, 1012, 1234
BtsIMutI CAGTG 5 cut(s) 409, 514, 939, 960, 1076
Cac8I GCNNGC 4 cut(s) 54, 735, 1057, 1211
CaiI CAGNNNCTG 3 cut(s) 938, 1052, 1298
CciI TCATGA 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 7 cut(s) 237, 320, 495, 891, 1098, 1271, 1477
CseI GACGC 1 cut(s) 944
Csp6I GTAC 2 cut(s) 1296, 1454
CviAII CATG 4 cut(s) 253, 317, 824, 883
CviQI GTAC 2 cut(s) 1296, 1454
DdeI CTNAG 5 cut(s) 99, 531, 931, 963, 1165
DpnI GATC 4 cut(s) 425, 536, 756, 929
DpnII GATC 4 cut(s) 423, 534, 754, 927
EaeI YGGCCR 1 cut(s) 987
Eam1104I CTCTTC 3 cut(s) 825, 1308, 1341
EarI CTCTTC 3 cut(s) 825, 1308, 1341
EciI GGCGGA 2 cut(s) 7, 1277
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 2 cut(s) 35, 380
Eco32I GATATC 1 cut(s) 1264
Eco47I GGWCC 2 cut(s) 237, 320
Eco53kI GAGCTC 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 1071
EcoICRI GAGCTC 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 495
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 6 cut(s) 61, 692, 871, 1100, 1291, 1456
EcoRV GATATC 1 cut(s) 1264
EcoT14I CCWWGG 1 cut(s) 882
EcoT38I GRGCYC 2 cut(s) 35, 380
ErhI CCWWGG 1 cut(s) 882
Esp3I CGTCTC 1 cut(s) 962
FaeI CATG 4 cut(s) 256, 320, 827, 886
FalI AAGNNNNNCTT 2 cut(s) 1305, 1337
FaqI GGGAC 2 cut(s) 250, 643
FatI CATG 4 cut(s) 252, 316, 823, 882
FauI CCCGC 2 cut(s) 89, 160
FauNDI CATATG 2 cut(s) 325, 333
FblI GTMKAC 1 cut(s) 1392
Fnu4HI GCNGC 6 cut(s) 85, 204, 465, 1068, 1191, 1214
FokI GGATG 4 cut(s) 734, 983, 1019, 1241
FriOI GRGCYC 2 cut(s) 35, 380
Fsp4HI GCNGC 6 cut(s) 85, 204, 465, 1068, 1191, 1214
FspBI CTAG 1 cut(s) 647
GluI GCNGC 6 cut(s) 85, 204, 465, 1068, 1191, 1214
GsaI CCCAGC 2 cut(s) 70, 1245
GsuI CTGGAG 1 cut(s) 1123
HaeIII GGCC 8 cut(s) 41, 56, 497, 893, 989, 1100, 1272, 1478
HapII CCGG 5 cut(s) 53, 118, 142, 170, 1480
HgaI GACGC 1 cut(s) 944
Hin1I GRCGYC 1 cut(s) 955
Hin1II CATG 4 cut(s) 256, 320, 827, 886
HincII GTYRAC 3 cut(s) 747, 1426, 1437
HindII GTYRAC 3 cut(s) 747, 1426, 1437
HindIII AAGCTT 2 cut(s) 445, 567
HpaI GTTAAC 1 cut(s) 1426
HpaII CCGG 5 cut(s) 53, 118, 142, 170, 1480
HphI GGTGA 1 cut(s) 1322
Hpy166II GTNNAC 6 cut(s) 216, 394, 747, 1393, 1426, 1437
Hpy188I TCNGA 8 cut(s) 165, 457, 520, 575, 677, 1168, 1304, 1381
Hpy188III TCNNGA 5 cut(s) 279, 662, 824, 1322, 1406
Hpy8I GTNNAC 6 cut(s) 216, 394, 747, 1393, 1426, 1437
HpyAV CCTTC 3 cut(s) 57, 961, 1403
HpyCH4III ACNGT 5 cut(s) 404, 509, 751, 1139, 1300
HpyCH4IV ACGT 1 cut(s) 808
HpyCH4V TGCA 5 cut(s) 160, 341, 708, 1055, 1067
HpyF10VI GCNNNNNNNGC 2 cut(s) 1073, 1097
HpyF3I CTNAG 5 cut(s) 99, 531, 931, 963, 1165
HpySE526I ACGT 1 cut(s) 808
Hsp92I GRCGYC 1 cut(s) 955
Hsp92II CATG 4 cut(s) 256, 320, 827, 886
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
KspAI GTTAAC 1 cut(s) 1426
Kzo9I GATC 4 cut(s) 423, 534, 754, 927
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 3 cut(s) 30, 383, 1064
Lsp1109I GCAGC 4 cut(s) 190, 1079, 1177, 1225
LweI GCATC 4 cut(s) 169, 238, 451, 1052
MaeI CTAG 1 cut(s) 647
MaeII ACGT 1 cut(s) 808
MaeIII GTNAC 1 cut(s) 640
MalI GATC 4 cut(s) 425, 536, 756, 929
MboI GATC 4 cut(s) 423, 534, 754, 927
MfeI CAATTG 2 cut(s) 382, 468
MflI RGATCY 2 cut(s) 534, 754
MhlI GDGCHC 3 cut(s) 35, 380, 1078
MlsI TGGCCA 1 cut(s) 989
MluCI AATT 9 cut(s) 268, 291, 382, 451, 468, 669, 974, 1177, 1382
MluNI TGGCCA 1 cut(s) 989
MlyI GAGTC 6 cut(s) 186, 563, 860, 1094, 1157, 1432
MmeI TCCRAC 1 cut(s) 655
Mox20I TGGCCA 1 cut(s) 989
MroNI GCCGGC 1 cut(s) 52
MroXI GAANNNNTTC 1 cut(s) 1317
MscI TGGCCA 1 cut(s) 989
MseI TTAA 4 cut(s) 563, 651, 1425, 1473
Msp20I TGGCCA 1 cut(s) 989
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 5 cut(s) 53, 118, 142, 170, 1480
MunI CAATTG 2 cut(s) 382, 468
Mva1269I GAATGC 2 cut(s) 1243, 1369
MvaI CCWGG 6 cut(s) 63, 694, 873, 1102, 1293, 1458
MwoI GCNNNNNNNGC 2 cut(s) 1073, 1097
NaeI GCCGGC 1 cut(s) 54
NciI CCSGG 4 cut(s) 118, 143, 171, 1480
NcoI CCATGG 1 cut(s) 882
NdeI CATATG 2 cut(s) 325, 333
NdeII GATC 4 cut(s) 423, 534, 754, 927
NgoMIV GCCGGC 1 cut(s) 52
NlaIII CATG 4 cut(s) 256, 320, 827, 886
NlaIV GGNNCC 4 cut(s) 7, 32, 76, 238
NmeAIII GCCGAG 1 cut(s) 46
PaeR7I CTCGAG 1 cut(s) 1071
PagI TCATGA 1 cut(s) 823
PciSI GCTCTTC 1 cut(s) 825
PctI GAATGC 2 cut(s) 1243, 1369
PdiI GCCGGC 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 1317
PfeI GAWTC 5 cut(s) 166, 356, 515, 689, 1318
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 6 cut(s) 86, 205, 466, 1069, 1192, 1215
PleI GAGTC 6 cut(s) 185, 562, 860, 1093, 1157, 1432
PpsI GAGTC 6 cut(s) 185, 562, 860, 1093, 1157, 1432
Psp124BI GAGCTC 1 cut(s) 380
Psp6I CCWGG 6 cut(s) 61, 692, 871, 1100, 1291, 1456
PspFI CCCAGC 2 cut(s) 66, 1241
PspGI CCWGG 6 cut(s) 61, 692, 871, 1100, 1291, 1456
PspN4I GGNNCC 4 cut(s) 7, 32, 76, 238
PspPI GGNCC 7 cut(s) 237, 320, 495, 891, 1098, 1271, 1477
PspXI VCTCGAGB 1 cut(s) 1071
PsrI GAACNNNNNNTAC 2 cut(s) 582, 614
PstI CTGCAG 1 cut(s) 1069
PstNI CAGNNNCTG 3 cut(s) 938, 1052, 1298
PsuI RGATCY 2 cut(s) 534, 754
RsaI GTAC 2 cut(s) 1297, 1455
RsaNI GTAC 2 cut(s) 1296, 1454
SacI GAGCTC 1 cut(s) 380
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 4 cut(s) 563, 651, 1425, 1473
SatI GCNGC 6 cut(s) 85, 204, 465, 1068, 1191, 1214
Sau3AI GATC 4 cut(s) 423, 534, 754, 927
Sau96I GGNCC 7 cut(s) 237, 320, 495, 891, 1098, 1271, 1477
SchI GAGTC 6 cut(s) 186, 563, 860, 1094, 1157, 1432
SduI GDGCHC 3 cut(s) 35, 380, 1078
SfaNI GCATC 4 cut(s) 169, 238, 451, 1052
SfcI CTRYAG 1 cut(s) 1065
Sfr274I CTCGAG 1 cut(s) 1071
SinI GGWCC 2 cut(s) 237, 320
SlaI CTCGAG 1 cut(s) 1071
SmlI CTYRAG 5 cut(s) 198, 368, 911, 1071, 1204
SmoI CTYRAG 5 cut(s) 198, 368, 911, 1071, 1204
Sse9I AATT 9 cut(s) 268, 291, 382, 451, 468, 669, 974, 1177, 1382
SsiI CCGC 9 cut(s) 18, 82, 85, 139, 153, 300, 464, 776, 1288
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 1 cut(s) 380
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 5 cut(s) 404, 509, 751, 1139, 1300
TaiI ACGT 1 cut(s) 811
TaqI TCGA 2 cut(s) 1072, 1161
TasI AATT 9 cut(s) 268, 291, 382, 451, 468, 669, 974, 1177, 1382
TauI GCSGC 2 cut(s) 87, 467
TfiI GAWTC 5 cut(s) 166, 356, 515, 689, 1318
Tru1I TTAA 4 cut(s) 563, 651, 1425, 1473
Tru9I TTAA 4 cut(s) 563, 651, 1425, 1473
TscAI CASTG 5 cut(s) 409, 514, 939, 967, 1083
TseI GCWGC 4 cut(s) 203, 1067, 1190, 1213
TspDTI ATGAA 6 cut(s) 498, 812, 843, 987, 1269, 1388
TspGWI ACGGA 2 cut(s) 808, 1185
TspRI CASTG 5 cut(s) 409, 514, 939, 967, 1083
VpaK11BI GGWCC 2 cut(s) 237, 320
XapI RAATTY 3 cut(s) 268, 451, 974
XhoI CTCGAG 1 cut(s) 1071
XmiI GTMKAC 1 cut(s) 1392
XmnI GAANNNNTTC 1 cut(s) 1317
XspI CTAG 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.