Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006070

Physical Location & Seq

Genomic Coordinates Forward (+)

32111807 .. 32115811

Gene Structure 4005 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1245600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGGGTTCCTGCTTGTCTGCAGAAAGCAGGAGCCCTCTGCCCGATTCGCCGTCGTCTCCCTCCTGGGGAGTCCGGCGGCGGAAGAGCTCGAAGAAGAGAATGGGCTCGAGAAATTCTTCCTTTGATTACCGCCGGGAAGAACGGCTGCACAGGATTCCCGAGAGAATGTTCTTCAATGGGTCCAGTGAAGTTGCATCTTTGTTTACACAGCAGGGCAAGAAAGGAACCAATCAAGATGCCATGATTGTGTGGGAGAATTTCGGTTCGAGAACAGACACAGTTTTCTGTGGTGTTTTCGATGGGCACGGTCCATTTGGTCATATGGTTGCTAAAAGAGTGAGGGACTCTCTTCCTCTGAAATTGAGTGCAAACTGGGAAGTGAACATAGTCAATGATGAAGTTCTCAAAGAGATTAGCCTCAACGCTGCAGGAAGCTTGAATTCAGATGATACTGCTTTTGTATCTGTTGATGAAGATTTCAGGCCCTCCGTGGATGTTGATGAAACAGAAAAAAACCCTGAGATCTTTCAGACACTGAGAGAGTCCTTTCTGAAGGCTTTTAAAGTTATGGACAGGGAGCTGCGAACAACACCAAGTATTGATTGCTTTTGTAGTGGGACAACAGCAGTAACTTTGATTAAACAGGGTCGGGATTTAATCATTGGAAATGTTGGAGACTCCCGAGCCGTTCTATGCACGAGAAGAATAGATGACACTCTAAGTGCAATTCAGTTGACTGTGGATCTCAAACCAAATCTTCCAGCGGAAGCAGAAAGAATTCGTAAGTGTAGAGGGCGTGTTTTTGCTCTTCAGGATGAACCTGAGGTTGCTCGGGTCTGGCTGCCAAACACTGACTCTCCTGGACTTGCCATGGCACGCGCTTTTGGAGATTTCTGCCTCAAGGATTTCGGCCTGATCTCTGTACCAGATGTATCCTATCGGCGTCTCACAGAGAGGGATGAATTTATAGTCCTGGCTACAGATGGGATTTGGGATGTCCTCTCTAACAAAGAAGTGGTAGACATTGTAGCAACAGCCCCAGCACGTTCTTCTGCAGCTCGAGCACTGGTAGAGACAGCAGTTCGAGCGTGGAGACAGAAGTACCCAACTTCCAAAGTAGATGACTGTGCTGTAGTTTGTCTCTTCCTTGACTTAGACTCAAACACAGTATCAACTGCTGCTAATAACGGGTCGAAGGAGAAGGAGCAGCCTACTACAGTGAATGAGGTAAATGCCGAAGAAGATCTCTCGGAGACAGCTTCTTTGGTCCACTCGGGGACTGTCCGAACTGCTGAAGAGATCCTTGCAGACGAGGAAGAAGACGAAGAAGAAGGAGGAAGCAAGGAGAATGCTGCAGATGATATGAATTCGGAACCAGGTGTAGAGTGGTCAGCTCTGGAAGGAGTTTCTCGAGTGAACACATTATTGAATCTGCCTAGGTTTACACCCACAAAAGAAGACAAGAAGGTGTGGAAGAAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

495

Amino Acids

54.79

Weight (kDa)

4.99

Isoelectric Point (pI)

43.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	177 - 362	3.2e-39	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006070)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02750 AT3G02750 AT3G02750 AT5G36250 AT5G36250 AT5G36250
fragaria_vesca	FvH4_6g00430 FvH4_6g00430 FvH4_6g00430 FvH4_6g00430 FvH4_6g00430
malus_domestica	MD04G1245600.v1.1 MD13G1061100.v1.1
prunus_persica	Prupe.6G362100_v2.0.a1
pyrus_communis	pycom04g21650 pycom12g23920
rosa_chinensis	RchiOBHm_Chr3g0447761
rosa_laevigata	RLG00000025973
rosa_multiflora	Rmu_ssc0000391.1_g000017
rosa_roxburghii	Rroxscaffold_6G00425960
rosa_rugosa	Rorug02G0605400 Rorug02G0605500
rosa_samantha	Rh3AG005100 Rh3BG004700 Rh3CG005100 Rh3DG005000
rosa_wichuraiana	Rw3G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1020
AccII	CGCG	1 cut(s)	879
AciI	CCGC	4 cut(s)	76, 79, 130, 764
AclWI	GGATC	2 cut(s)	750, 1294
AcsI	RAATTY	6 cut(s)	112, 256, 439, 777, 962, 1366
AcuI	CTGAAG	3 cut(s)	572, 794, 1314
AcyI	GRCGYC	1 cut(s)	943
AfaI	GTAC	2 cut(s)	924, 1103
AfiI	CCNNNNNNNGG	1 cut(s)	65
AgsI	TTSAA	3 cut(s)	175, 439, 1429
AjnI	CCWGG	4 cut(s)	62, 859, 972, 1375
AluBI	AGCT	6 cut(s)	87, 435, 580, 1058, 1259, 1394
AluI	AGCT	6 cut(s)	87, 435, 580, 1058, 1259, 1394
Alw21I	GWGCWC	2 cut(s)	89, 1066
Alw26I	GTCTC	7 cut(s)	60, 669, 950, 1067, 1087, 1145, 1247
AlwI	GGATC	2 cut(s)	750, 1294
AlwNI	CAGNNNCTG	1 cut(s)	535
Ama87I	CYCGRG	7 cut(s)	106, 158, 681, 831, 1059, 1273, 1410
AoxI	GGCC	2 cut(s)	482, 910
ApeKI	GCWGC	8 cut(s)	145, 425, 580, 841, 1055, 1178, 1207, 1352
ApoI	RAATTY	6 cut(s)	112, 256, 439, 777, 962, 1366
ArsI	GACNNNNNNTTYG	2 cut(s)	1246, 1278
Asp700I	GAANNNNTTC	2 cut(s)	115, 777
AspA2I	CCTAGG	1 cut(s)	1436
AspLEI	GCGC	1 cut(s)	881
AspS9I	GGNCC	4 cut(s)	180, 308, 483, 1267
AsuC2I	CCSGG	1 cut(s)	134
AvaI	CYCGRG	7 cut(s)	106, 158, 681, 831, 1059, 1273, 1410
AvaII	GGWCC	3 cut(s)	180, 308, 1267
AvrII	CCTAGG	1 cut(s)	1436
AxyI	CCTNAGG	1 cut(s)	822
BaeGI	GKGCMC	1 cut(s)	306
BaeI	ACNNNNGTAYC	2 cut(s)	1085, 1118
BanII	GRGCYC	3 cut(s)	35, 89, 107
BauI	CACGAG	1 cut(s)	697
BbsI	GAAGAC	2 cut(s)	1326, 1464
Bbv12I	GWGCWC	2 cut(s)	89, 1066
BbvI	GCAGC	8 cut(s)	132, 412, 567, 828, 1067, 1165, 1219, 1339
BccI	CCATC	2 cut(s)	293, 977
BceAI	ACGGC	3 cut(s)	34, 158, 671
BciT130I	CCWGG	4 cut(s)	64, 861, 974, 1377
BciVI	GTATCC	1 cut(s)	943
BcnI	CCSGG	1 cut(s)	134
BcoDI	GTCTC	7 cut(s)	60, 669, 950, 1067, 1087, 1145, 1247
BfaI	CTAG	1 cut(s)	1437
BfmI	CTRYAG	7 cut(s)	18, 426, 978, 1053, 1131, 1215, 1353
BfuI	GTATCC	1 cut(s)	943
BglII	AGATCT	2 cut(s)	522, 1243
BisI	GCNGC	9 cut(s)	77, 146, 426, 581, 842, 1056, 1179, 1208, 1353
BlnI	CCTAGG	1 cut(s)	1436
BlsI	GCNGC	9 cut(s)	78, 147, 427, 582, 843, 1057, 1180, 1209, 1354
Bme1390I	CCNGG	5 cut(s)	64, 134, 861, 974, 1377
Bme18I	GGWCC	3 cut(s)	180, 308, 1267
BmeT110I	CYCGRG	7 cut(s)	106, 158, 681, 831, 1059, 1273, 1410
BmgT120I	GGNCC	4 cut(s)	180, 308, 483, 1267
BmiI	GGNNCC	5 cut(s)	7, 32, 181, 226, 1374
BmrFI	CCNGG	5 cut(s)	64, 134, 861, 974, 1377
BmrI	ACTGGG	1 cut(s)	382
BmsI	GCATC	2 cut(s)	203, 226
BmuI	ACTGGG	1 cut(s)	382
BpiI	GAAGAC	2 cut(s)	1326, 1464
BplI	GAGNNNNNCTC	2 cut(s)	331, 363
BpuEI	CTTGAG	1 cut(s)	884
BpuMI	CCSGG	1 cut(s)	134
BsaHI	GRCGYC	1 cut(s)	943
BsaJI	CCNNGG	4 cut(s)	63, 489, 870, 1436
BsaXI	ACNNNNNCTCC	2 cut(s)	841, 871
Bsc4I	CCNNNNNNNGG	1 cut(s)	65
Bse1I	ACTGG	3 cut(s)	183, 377, 1071
Bse21I	CCTNAGG	1 cut(s)	822
BseBI	CCWGG	4 cut(s)	64, 861, 974, 1377
BseDI	CCNNGG	4 cut(s)	63, 489, 870, 1436
BseGI	GGATG	4 cut(s)	499, 820, 964, 1000
BseLI	CCNNNNNNNGG	1 cut(s)	65
BseMII	CTCAG	3 cut(s)	510, 527, 813
BseNI	ACTGG	3 cut(s)	183, 377, 1071
BseSI	GKGCMC	1 cut(s)	306
BseXI	GCAGC	8 cut(s)	132, 412, 567, 828, 1067, 1165, 1219, 1339
BseYI	CCCAGC	1 cut(s)	1039
BsgI	GTGCAG	1 cut(s)	131
Bsh1236I	CGCG	1 cut(s)	879
BshFI	GGCC	2 cut(s)	484, 912
BsiHKAI	GWGCWC	2 cut(s)	89, 1066
BsiHKCI	CYCGRG	7 cut(s)	106, 158, 681, 831, 1059, 1273, 1410
BsiSI	CCGG	2 cut(s)	73, 133
BslFI	GGGAC	3 cut(s)	356, 631, 1291
BslI	CCNNNNNNNGG	1 cut(s)	65
BsmAI	GTCTC	7 cut(s)	60, 669, 950, 1067, 1087, 1145, 1247
BsmBI	CGTCTC	2 cut(s)	60, 950
BsmFI	GGGAC	3 cut(s)	356, 631, 1291
BsmI	GAATGC	1 cut(s)	1354
BsnI	GGCC	2 cut(s)	484, 912
BsoBI	CYCGRG	7 cut(s)	106, 158, 681, 831, 1059, 1273, 1410
Bsp1286I	GDGCHC	5 cut(s)	35, 89, 107, 306, 1066
Bsp143I	GATC	5 cut(s)	522, 742, 915, 1243, 1299
Bsp19I	CCATGG	1 cut(s)	870
BspACI	CCGC	4 cut(s)	76, 79, 130, 764
BspANI	GGCC	2 cut(s)	484, 912
BspCNI	CTCAG	3 cut(s)	511, 528, 814
BspFNI	CGCG	1 cut(s)	879
BspLI	GGNNCC	5 cut(s)	7, 32, 181, 226, 1374
BspMAI	CTGCAG	4 cut(s)	22, 430, 1057, 1357
BspPI	GGATC	2 cut(s)	750, 1294
BspQI	GCTCTTC	2 cut(s)	77, 813
BsrI	ACTGG	3 cut(s)	183, 377, 1071
BssECI	CCNNGG	4 cut(s)	63, 489, 870, 1436
BssMI	GATC	5 cut(s)	522, 742, 915, 1243, 1299
BssNI	GRCGYC	1 cut(s)	943
BssSI	CACGAG	1 cut(s)	697
BssT1I	CCWWGG	2 cut(s)	870, 1436
Bst2BI	CACGAG	1 cut(s)	697
Bst2UI	CCWGG	4 cut(s)	64, 861, 974, 1377
Bst4CI	ACNGT	7 cut(s)	280, 308, 739, 1127, 1168, 1219, 1282
Bst6I	CTCTTC	6 cut(s)	77, 89, 354, 813, 1148, 1290
BstACI	GRCGYC	1 cut(s)	943
BstC8I	GCNNGC	1 cut(s)	877
BstDEI	CTNAG	5 cut(s)	519, 536, 719, 822, 1153
BstDSI	CCRYGG	2 cut(s)	489, 870
BstF5I	GGATG	4 cut(s)	499, 820, 964, 1000
BstFNI	CGCG	1 cut(s)	879
BstHHI	GCGC	1 cut(s)	881
BstKTI	GATC	5 cut(s)	525, 745, 918, 1246, 1302
BstMAI	GTCTC	7 cut(s)	60, 669, 950, 1067, 1087, 1145, 1247
BstMBI	GATC	5 cut(s)	522, 742, 915, 1243, 1299
BstMWI	GCNNNNNNNGC	3 cut(s)	46, 1061, 1085
BstNI	CCWGG	4 cut(s)	64, 861, 974, 1377
BstSCI	CCNGG	5 cut(s)	62, 132, 859, 972, 1375
BstSFI	CTRYAG	7 cut(s)	18, 426, 978, 1053, 1131, 1215, 1353
BstSLI	GKGCMC	1 cut(s)	306
BstUI	CGCG	1 cut(s)	879
BstV1I	GCAGC	8 cut(s)	132, 412, 567, 828, 1067, 1165, 1219, 1339
BstV2I	GAAGAC	2 cut(s)	1326, 1464
BstX2I	RGATCY	4 cut(s)	522, 742, 1243, 1299
BstYI	RGATCY	4 cut(s)	522, 742, 1243, 1299
Bsu36I	CCTNAGG	1 cut(s)	822
BsuI	GTATCC	1 cut(s)	943
BsuRI	GGCC	2 cut(s)	484, 912
BtgI	CCRYGG	2 cut(s)	489, 870
BtsCI	GGATG	4 cut(s)	499, 820, 964, 1000
BtsIMutI	CAGTG	5 cut(s)	190, 533, 849, 1064, 1224
Cac8I	GCNNGC	1 cut(s)	877
CaiI	CAGNNNCTG	1 cut(s)	535
CfoI	GCGC	1 cut(s)	881
Cfr13I	GGNCC	4 cut(s)	180, 308, 483, 1267
CseI	GACGC	1 cut(s)	932
CsiI	ACCWGGT	1 cut(s)	1375
Csp6I	GTAC	2 cut(s)	923, 1102
CviAII	CATG	2 cut(s)	241, 871
CviQI	GTAC	2 cut(s)	923, 1102
DdeI	CTNAG	5 cut(s)	519, 536, 719, 822, 1153
DpnI	GATC	5 cut(s)	524, 744, 917, 1245, 1301
DpnII	GATC	5 cut(s)	522, 742, 915, 1243, 1299
DraI	TTTAAA	1 cut(s)	562
Eam1104I	CTCTTC	6 cut(s)	77, 89, 354, 813, 1148, 1290
EarI	CTCTTC	6 cut(s)	77, 89, 354, 813, 1148, 1290
EciI	GGCGGA	1 cut(s)	94
Ecl136II	GAGCTC	1 cut(s)	87
Eco130I	CCWWGG	2 cut(s)	870, 1436
Eco24I	GRGCYC	3 cut(s)	35, 89, 107
Eco47I	GGWCC	3 cut(s)	180, 308, 1267
Eco53kI	GAGCTC	1 cut(s)	87
Eco57I	CTGAAG	3 cut(s)	572, 794, 1314
Eco81I	CCTNAGG	1 cut(s)	822
Eco88I	CYCGRG	7 cut(s)	106, 158, 681, 831, 1059, 1273, 1410
EcoICRI	GAGCTC	1 cut(s)	87
EcoO109I	RGGNCCY	1 cut(s)	483
EcoRI	GAATTC	3 cut(s)	439, 777, 1366
EcoRII	CCWGG	4 cut(s)	62, 859, 972, 1375
EcoT14I	CCWWGG	2 cut(s)	870, 1436
EcoT38I	GRGCYC	3 cut(s)	35, 89, 107
ErhI	CCWWGG	2 cut(s)	870, 1436
Esp3I	CGTCTC	2 cut(s)	60, 950
FaeI	CATG	2 cut(s)	244, 874
FaiI	YATR	9 cut(s)	242, 321, 323, 386, 569, 694, 872, 968, 1364
FalI	AAGNNNNNCTT	2 cut(s)	1287, 1319
FaqI	GGGAC	3 cut(s)	356, 631, 1291
FatI	CATG	2 cut(s)	240, 870
FauNDI	CATATG	1 cut(s)	321
FblI	GTMKAC	1 cut(s)	1020
Fnu4HI	GCNGC	9 cut(s)	77, 146, 426, 581, 842, 1056, 1179, 1208, 1353
FokI	GGATG	4 cut(s)	506, 827, 971, 1007
FriOI	GRGCYC	3 cut(s)	35, 89, 107
Fsp4HI	GCNGC	9 cut(s)	77, 146, 426, 581, 842, 1056, 1179, 1208, 1353
FspBI	CTAG	1 cut(s)	1437
GlaI	GCGC	1 cut(s)	880
GluI	GCNGC	9 cut(s)	77, 146, 426, 581, 842, 1056, 1179, 1208, 1353
GsaI	CCCAGC	1 cut(s)	1043
HaeIII	GGCC	2 cut(s)	484, 912
HapII	CCGG	2 cut(s)	73, 133
HgaI	GACGC	1 cut(s)	932
HhaI	GCGC	1 cut(s)	881
Hin1I	GRCGYC	1 cut(s)	943
Hin1II	CATG	2 cut(s)	244, 874
Hin6I	GCGC	1 cut(s)	879
HinP1I	GCGC	1 cut(s)	879
HincII	GTYRAC	1 cut(s)	735
HindII	GTYRAC	1 cut(s)	735
HindIII	AAGCTT	1 cut(s)	433
HinfI	GANTC	9 cut(s)	44, 69, 154, 344, 542, 677, 854, 1157, 1429
HpaII	CCGG	2 cut(s)	73, 133
Hpy166II	GTNNAC	7 cut(s)	204, 382, 735, 1021, 1270, 1417, 1443
Hpy188I	TCNGA	7 cut(s)	357, 445, 531, 552, 1252, 1286, 1372
Hpy188III	TCNNGA	9 cut(s)	108, 158, 233, 267, 650, 681, 812, 1397, 1410
Hpy8I	GTNNAC	7 cut(s)	204, 382, 735, 1021, 1270, 1417, 1443
Hpy99I	CGWCG	1 cut(s)	55
HpyAV	CCTTC	7 cut(s)	547, 1189, 1195, 1325, 1394, 1459, 1471
HpyCH4III	ACNGT	7 cut(s)	280, 308, 739, 1127, 1168, 1219, 1282
HpyCH4IV	ACGT	1 cut(s)	1045
HpyF10VI	GCNNNNNNNGC	3 cut(s)	46, 1061, 1085
HpyF3I	CTNAG	5 cut(s)	519, 536, 719, 822, 1153
HpySE526I	ACGT	1 cut(s)	1045
Hsp92I	GRCGYC	1 cut(s)	943
Hsp92II	CATG	2 cut(s)	244, 874
HspAI	GCGC	1 cut(s)	879
Kzo9I	GATC	5 cut(s)	522, 742, 915, 1243, 1299
LguI	GCTCTTC	2 cut(s)	77, 813
LmnI	GCTCC	3 cut(s)	30, 577, 1204
Lsp1109I	GCAGC	8 cut(s)	132, 412, 567, 828, 1067, 1165, 1219, 1339
LweI	GCATC	2 cut(s)	203, 226
MabI	ACCWGGT	1 cut(s)	1375
MaeI	CTAG	1 cut(s)	1437
MaeII	ACGT	1 cut(s)	1045
MaeIII	GTNAC	1 cut(s)	628
MalI	GATC	5 cut(s)	524, 744, 917, 1245, 1301
MboI	GATC	5 cut(s)	522, 742, 915, 1243, 1299
MflI	RGATCY	4 cut(s)	522, 742, 1243, 1299
MhlI	GDGCHC	5 cut(s)	35, 89, 107, 306, 1066
MluCI	AATT	8 cut(s)	112, 256, 359, 439, 726, 777, 962, 1366
MlyI	GAGTC	6 cut(s)	78, 338, 551, 671, 848, 1151
MmeI	TCCRAC	1 cut(s)	652
MroXI	GAANNNNTTC	2 cut(s)	115, 777
MseI	TTAA	3 cut(s)	561, 639, 656
MslI	CAYNNNNRTG	1 cut(s)	245
MspA1I	CMGCKG	1 cut(s)	764
MspI	CCGG	2 cut(s)	73, 133
MspR9I	CCNGG	5 cut(s)	64, 134, 861, 974, 1377
Mva1269I	GAATGC	1 cut(s)	1354
MvaI	CCWGG	4 cut(s)	64, 861, 974, 1377
MvnI	CGCG	1 cut(s)	879
MwoI	GCNNNNNNNGC	3 cut(s)	46, 1061, 1085
NciI	CCSGG	1 cut(s)	134
NcoI	CCATGG	1 cut(s)	870
NdeI	CATATG	1 cut(s)	321
NdeII	GATC	5 cut(s)	522, 742, 915, 1243, 1299
NlaIII	CATG	2 cut(s)	244, 874
NlaIV	GGNNCC	5 cut(s)	7, 32, 181, 226, 1374
PaeR7I	CTCGAG	3 cut(s)	106, 1059, 1410
PciSI	GCTCTTC	2 cut(s)	77, 813
PctI	GAATGC	1 cut(s)	1354
PdmI	GAANNNNTTC	2 cut(s)	115, 777
PfeI	GAWTC	3 cut(s)	44, 154, 1429
PfoI	TCCNGGA	1 cut(s)	859
PkrI	GCNGC	9 cut(s)	78, 147, 427, 582, 843, 1057, 1180, 1209, 1354
PleI	GAGTC	6 cut(s)	77, 338, 550, 671, 848, 1151
PpsI	GAGTC	6 cut(s)	77, 338, 550, 671, 848, 1151
Psp124BI	GAGCTC	1 cut(s)	89
Psp6I	CCWGG	4 cut(s)	62, 859, 972, 1375
PspFI	CCCAGC	1 cut(s)	1039
PspGI	CCWGG	4 cut(s)	62, 859, 972, 1375
PspN4I	GGNNCC	5 cut(s)	7, 32, 181, 226, 1374
PspPI	GGNCC	4 cut(s)	180, 308, 483, 1267
PspXI	VCTCGAGB	1 cut(s)	1059
PstI	CTGCAG	4 cut(s)	22, 430, 1057, 1357
PstNI	CAGNNNCTG	1 cut(s)	535
PsuI	RGATCY	4 cut(s)	522, 742, 1243, 1299
RsaI	GTAC	2 cut(s)	924, 1103
RsaNI	GTAC	2 cut(s)	923, 1102
RseI	CAYNNNNRTG	1 cut(s)	245
SacI	GAGCTC	1 cut(s)	89
SapI	GCTCTTC	2 cut(s)	77, 813
SaqAI	TTAA	3 cut(s)	561, 639, 656
SatI	GCNGC	9 cut(s)	77, 146, 426, 581, 842, 1056, 1179, 1208, 1353
Sau3AI	GATC	5 cut(s)	522, 742, 915, 1243, 1299
Sau96I	GGNCC	4 cut(s)	180, 308, 483, 1267
SchI	GAGTC	6 cut(s)	78, 338, 551, 671, 848, 1151
ScrFI	CCNGG	5 cut(s)	64, 134, 861, 974, 1377
SduI	GDGCHC	5 cut(s)	35, 89, 107, 306, 1066
SexAI	ACCWGGT	1 cut(s)	1375
SfaNI	GCATC	2 cut(s)	203, 226
SfcI	CTRYAG	7 cut(s)	18, 426, 978, 1053, 1131, 1215, 1353
Sfr274I	CTCGAG	3 cut(s)	106, 1059, 1410
SinI	GGWCC	3 cut(s)	180, 308, 1267
SlaI	CTCGAG	3 cut(s)	106, 1059, 1410
SmiMI	CAYNNNNRTG	1 cut(s)	245
SmlI	CTYRAG	4 cut(s)	106, 899, 1059, 1410
SmoI	CTYRAG	4 cut(s)	106, 899, 1059, 1410
Sse9I	AATT	8 cut(s)	112, 256, 359, 439, 726, 777, 962, 1366
SsiI	CCGC	4 cut(s)	76, 79, 130, 764
SspMI	CTAG	1 cut(s)	1437
SstI	GAGCTC	1 cut(s)	89
StyD4I	CCNGG	5 cut(s)	62, 132, 859, 972, 1375
StyI	CCWWGG	2 cut(s)	870, 1436
TaaI	ACNGT	7 cut(s)	280, 308, 739, 1127, 1168, 1219, 1282
TaiI	ACGT	1 cut(s)	1048
TaqI	TCGA	8 cut(s)	89, 107, 266, 297, 1060, 1084, 1193, 1411
TasI	AATT	8 cut(s)	112, 256, 359, 439, 726, 777, 962, 1366
TauI	GCSGC	1 cut(s)	79
TfiI	GAWTC	3 cut(s)	44, 154, 1429
Tru1I	TTAA	3 cut(s)	561, 639, 656
Tru9I	TTAA	3 cut(s)	561, 639, 656
TscAI	CASTG	5 cut(s)	190, 540, 856, 1071, 1224
TseI	GCWGC	8 cut(s)	145, 425, 580, 841, 1055, 1178, 1207, 1352
TspDTI	ATGAA	6 cut(s)	411, 486, 516, 831, 975, 1379
TspGWI	ACGGA	1 cut(s)	478
TspRI	CASTG	5 cut(s)	190, 540, 856, 1071, 1224
VpaK11BI	GGWCC	3 cut(s)	180, 308, 1267
XapI	RAATTY	6 cut(s)	112, 256, 439, 777, 962, 1366
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1383
XhoI	CTCGAG	3 cut(s)	106, 1059, 1410
XmaJI	CCTAGG	1 cut(s)	1436
XmiI	GTMKAC	1 cut(s)	1020
XmnI	GAANNNNTTC	2 cut(s)	115, 777
XspI	CTAG	1 cut(s)	1437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.