AT5G40480

Bacterial Ig-like domain (group 2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014163
Physical Location & Seq
Forward (+)
16213036 .. 16224177
11142 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40480.1

Sequence Viewer

Length: 5772 bp
ATGGTTCCCGTCTCGTTCTGCTTCTTCTTTCTCCTTCTCTTGCTCTCTGCTGGAGAGAGTTCATCGCAATTAGTTTCTGGTCCACACATCACCGATGTTAACATACTCTTACCTCCCAAGATGAAGAATCCGGTTGAGTATCGCCTTCAAGGAAGTGATGGCTGCTTCAAATGGTCATGGGATCATCATGATATTCTATCAGTCACACCTGAGTTTAATTCGAGCAGTCATTGTTCGACCAGCGCGCGTCTCAGATCGATTTCTCCGTATAGTGGTAGAAAGGAGACTGCAGTTTATGCCACTGATATACAGACTGGGATGGTGATTCGCTGCAAGGTCTTTATTGACAACTTCTCACGGATTCAGATTTTTCATAATTCTATCAAGCTTGATCTTGATGGTCTTTCCATGCTTAGAGTCCGCGCTTTTGATAATGAAGATAACGAGTTCTCCTCACTGGTGGGTCTACAATTCATTTGGAAGCTAATGCCTGAGAGTGGTGGATCAACACATCATCTTGCTCATGTGCCTTTGAAGGAATCTCCGTTGACTGACTGTGGAGGACTGTGTGGCTACTTAGATATCCAGAAAAAGCTTGAAGATAGTGGTGTATTTGCAGATTTGTTTGTTGTGAAGGGAACTAAAATTGGACATGAGAAGGTTTCAGTTCATCTGCTTGAGGCTCCTCTGACCCATATAGCTGATGAAATTGTATTAACGGTTGCAGAAGCCATGTCTCTTGAACCTCGCTCACCTGTCTATGTCCTGATGGGAGCTTCTTTTGGTTACACGTTAAAGGTCATGCGTGGAAATGTTCCTCAAGCCGTGGATCTACCATCACCACATCATAGATGGTCTGTGTTAAACTCTTCTGTTGCTCAAGTGGATTCTTTGATAGGTCTGACTAAAGCGTTAAGCTTGGGGGTAACAACAGTTGTTGTTGAGGATACCCGGGTCGCTGGCCACATTCAAGGATCATCTATTAATGTTGTCACTCCAGATACTCTTATCTTGTACATATCACCTTGGTCAATGTCAGGGGATCTTATTACAGAATCCAAACCTTTTCCATCATCTATGCATTGGTATGTGGTGTCTGGTCGTCAATATCTAATTCAAATGAAGATTTTCTCTGGAAGACCTGATGCACATGAAATCTATATAACTGAGACGGATGACATAAAACTGTATGGAAAAGATTCAGATTATTGGAAAATCGTCTCACTGCCAGATGAACTTTCCTCTGAATATGGTCAGCGAAATTCTAGAATTTTGAACGCAATCTCACCAGGATTAGGAGAGCTGACATCTACATTGACTTACTTCAGTGGGCATCAAGAGTCAAAAGAGGTTCTCAAGGTTGTCCAAGAAATTAGGGTTTGTGAAAAAGTGCAGTTCACATTGAACAGTGAAGATGACACACCTAAGGTTCTACTCCCATGGACCCCTGCTGTTTATCAGGAGATGGAGCTAATTGTGACAGGAGGTTGTGCAAAAGCATCGAGTGACTACAAGTGGTTTACTTCAGATATAAGCATTTTGTCGGTGTCAGCTTATGGAATTATCCAGGCAAAGAGGCCCGGTATAGCCACTGTGAAGGTGGTGTCGACTTTCGATTCACAAAATTTTGATGAGGTTATTGTTGAAGTTTCCATTCCATCCTCTATGGTTATGTTGCAAAACTTCCCAGTAGAGACAGTTGTTGGATCACACCTGAAAGCTGCTGTCACAATGAAGGCTTTAAATGGTGCAACGTTCTCTAGATGTGATGCTTTTAATTCATTGATAAAGTGGAAGACGGGGAGTGAGTCTTTTGTTATTGTTAATGCAACATCTGAGATGATGATGTTGGACGAACTAAGAAGCATGGACTCCAGCCCTCCTTGTTCACGGGCTTCTATATATACTGCATCTACTGGCCGCACAGTATTACAAGCAACTCTGGCAAAAGAGTTCCATTATTTTGATAAGTCTTTGAGTGAATCAATAGATTTGAAAGCAACTTTAACTATCGGGGCCTATCTGCCACTTTCTGTCAGACAAGATAGTGACGGGAATCATCATGGTGGATATTGGTTTGATAAGGCACAAGAGGAAACTGATTTTGGTGTTAGTAAGCTATATCTTGTACCTGGTACATATGTTGACGTTATGCTTCTGGGTGGACCTGAAAGGTGGGATGACAATGTTGAGTTCACCGAGACTGTGAAGACATTATATGAAGATGAGGAGGATCTTACAAGCAGAGTTAACGTCCATCATGAGGTTGACCGCCGTGCTAACATGTATAGAATTTCATGCCAGAAGCTTGGCTCCTATAAACTGGTCTTCTTGCGTGGGAATCTGCTGGGTATAGACCATCCTGTACCTGCAGTTGCAGAAGCTTTATTGTCTGTTCATTGCAGCTTACCATCCTCTGTTGTGCTCATTGTTGATGAACCTGTTAACAAGCTTGATGTGATTAGAGCTGCTAGTCAAGCTGACAGGGCACCAGGACGACTTCGTGTAACTCCTGTGACTGTGGCAAATGGGCAAATAATTCGTGTGGCTGCTGTTGGTATTAGCGAGTTTGGAGAAGCTTTTTCAAACTCCTCAACTCTTTCTCTCAGGTGGGAATTGACCAGTTGCAACAATTTGGCATACTGGGACGATGATTATAACTCAAAAATGACAAAGTCTGGCTGGGAGAGATTTCTGGCTCTACGCAATGAATCAGGATTGTGCACTGTTCGAGCCACCGTTTCTGGTATTGATTATTCTTTCAAAAGCCAATACTCAACTCTGCTTCCACAAGGTTCTGAAAGTACTCTCACTGATGCTGTACGCTTACAGTTGGTTTCCACACTAAGAGTCACACCAGAGTTCAACCTGGTCTTTTTCAATCCTAATGCCAAGGTAAACTTGTCAATGACTGGAGGGAGCTGTTTGTGGGAAGCAGTTGTGAATAATTCTCGTGTAGCAGAAGTTATTCGACCCCCATCTGGCCTGCAATGCTCGCAAATGATGCTATCTCCAAAAGGGTTGGGGACTACAATCGTGACAGTATACGACATTGGAGTTTCTCCTCCACTTTCAGCTCTTGCGTTGATAAAAGTTGCAGATGTGGACTGGATAAAGATTGCATCTGGGGATGAGATAAGTATAATGGAAGGAAGTACACACTCCATTGATCTCTTGACTGGCATTGACGATGGAATGACCTTTGATTCTTCCCAGTACTCACTTATGGACATTATGGTGCATATTGAGGATGATCTCGTGGAGCATGTCACTGTTGATGAGGATTCACTTTCTGTTGGTGAACACGTGGCAACTTCCTCTTTTAAAATTGCTGCTAGACGACTGGGAATCACAACCCTATATGTCAGCGCAAGACAACAATCAGGAGGTAAAGTGCTAAGCCAAACTATTAAAGTCGAAGTTTATTCCCCACCAAGACTTCACCCACAAGGAATATTTCTTGTTCCTGGTGCTTCCTATGTGCTCACTATTGAAGGAGGCCCGACCATGAATGTTTCTGTTGACTACACCACTGTGGATAACGAAGTTGCAAAGATCGAAAAATCAGGACGCCTATATGCAACATCTCCCGGCAACACGACAATCTATGCAACAATTTACGGGAGTGAAGGTGCTGTAATTTGTCAGGCAATTGGAAATGCCGAAGTAGGACTTCCTGCTACTGCAATGCTGGTAGCTCAAAGTGACACTGTGGCCGTTGGCCATGAAATGCCTGTATCCCCATCATTTCCTGAGGGTGACCTTTTATCTTTTTATGAGCTCTGCAGCGCTTACAAATGGACTATTGAAGATGAAAAGGTGTTAATTTTCATTGCATCATCCATCAATGTGGAAGAGAATGCTGGCTTTGTTAATGTCGTCCAAGGAAGATCAGCTGGCAAAACCAGAGTTACAATTGCCTTCTCATGTGATTTCGTGTCTCCTGGCTTGTACTCTGAATCTAGAACATATGAAGCGTCAATGATTTTATCTGTTGTGCCTGATCTCCCTCTTTCGCTTGGGGCTCCTATGACTTGGGTTCTTCCGCCTTTTTATACATCATCTGGTCTATTACCTTCATCTTCAGAGCCACAGAAGCATAGAGATGGACAAAGTCACAGAGGCAATATAGTCTACTCCATATTGAAAGACTGCAGCTCTCGGGCTGATTTTGAACGAGATACCATATCAATTAATGGCGGGAGTGTCAAAACAACTGATAGCAACAATGTTGCATGTATTCAAGCCAAAGACCGCACAAGTGGAAGGATTGAGATTGCTGCTTGCGTGAGAGTTGCAGAGGTTGCTCAAATAAGAATGAAGAGTGAGGGGATCCCTTTCCACGTGATAGATCTTGCTGTTGGCGGTGAACTTGAACTTCCAATAAATTACTATGACACTTTAGGAATCCCTTTTCTCGAAGCACATGGTGTTACTACATACAATGTCGAAACCAATCATCGTGATGTTGTATTTATTAAGACTGTCAATGACCAACCAAGTGCCTACATTAAGGGAATAAAACATGGCAAAGCTCTCATTCGGGTTTCCATTGGTGATAATCTCAGGAAATCAGATTACGTTCTGGTTTCAGTTGGGGCGCATATCTTTCCTCAAAACCCGGTCATTCACACTGGAAATTTGTTAAATTTCAGCATAACAGGGGCTGATAATGAAGTCACTGGTCAATGGTTTACATCAAACAGAAGTGTCATATCCGTCAATGTGGCATCTGGCCAAGCTAAAGCGATCAGCCAAGGCTCAACTCACGTTACATTTAAAGGTCATGGTTTGAAACTGCAAACAAAAGTCACAGTGCTATTTGGAAACACCATATATGTCGACTCTCCAGGAGAAACACTGACAAATGTCCACGTCCCTGCAGAAGGATATAAGTTTCCAGTGAAATTCAGGGAGAACAAGTTTGCAGTCACTGAACATGGAAACAAGGCCACGTTTAACTGCCAAGTAGACCCTCCGTTTATAGGATACACAAAGCCATGGATGGATCTCGATACGGGCAACACTTATTGTCTTTTCTTCCCATACTCACCAGAGCATCTTGTTCACTCCATGTCTATAACAAAAGACATGAAACCTCATGTGTCATTCTCCGTTGATGCTTCACTTAAAGAAGCTCGACGTGTTTCGGGATCTGCATCTGCACTTCTTATAGGAGGGTTTTCTGTAACAGGGCCAGATAAGCTGAATATAAACCCGGATTCAAACACGACCATCATTTCATTAGTGGGGAACACCGATGTTCAAATTCACTGCCGCAACAAAGGTAGGTTATCAATCAGCTTGATTAAAAGAGACGATTTTGGTATCGCAGGGCATGCACAATACAAGGTAAATGTGTTAAGATCAGAGCAATTCACAGATAGAATCATCATCACTCTACCTGCTACTGGCCAAATTGTAGAAATCGATGTCTGCTATGACACAGGAGAGTCACTAGTAGCTTCGTCTAAAGATGGTTACTCTGTTTTGTTGAAGATACTATGGGGCGTTTTGGTGTTGGTTGTATCAGTGATCATCTTGATGAAAGTGATAGATCGACAAGTGCCCACAGGAGCTACTGGAACGGCAACATACAGTGGAAATGCAGCACAGGGAACACCAGAAAGAAGAAGCGGCACAGTTATATACCATGAGGAATCTCCAAGAACACCATCACCATTTATGGAGTATGTGAAGAGAACAGTGGATGAAACTCCCTACTACAGAAGAGAAGGAAGAAGAAGGTTTAATCCTCAAAACACTATGTAG

Protein Analysis

1923

Amino Acids

210.66

Weight (kDa)

5.65

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_NUP210_1st PF22967 33 - 116 3.7e-17 NUP210 Ig-like domain 1
Ig_NUP210_2nd PF22969 137 - 234 5.3e-10 NUP210 Ig-like domain 2
Ig_NUP210_7th PF22962 705 - 802 3.6e-17 NUP210 Ig-like domain 7
Ig_GP210_15th PF24425 1609 - 1725 2.3e-46 Nuclear pore complex protein GP210, Ig-like domain
Ig_GP210_16th PF24427 1733 - 1815 1.2e-32 Nuclear pore complex protein GP210, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10590 AT5G40480
fragaria_vesca FvH4_6g51430
malus_domestica MD17G1026700.v1.1
prunus_persica Prupe.3G292000_v2.0.a1 Prupe.3G292000_v2.0.a1
pyrus_communis pycom17g02180
rosa_chinensis RchiOBHm_Chr2g0173101
rosa_laevigata RLG00000022158
rosa_multiflora Rmu_sc0011236.1_g000011
rosa_roxburghii Rroxscaffold_2G00079240
rosa_rugosa Rorug02G0565200
rosa_samantha Rh2AG645300 Rh2BG656000 Rh2CG621100 Rh2DG669400
rosa_wichuraiana Rw2G053000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2658
Acc36I ACCTGC 2 cut(s) 2377, 5413
AccB1I GGYRCC 1 cut(s) 2488
AccI GTMKAC 6 cut(s) 466, 1607, 3045, 4134, 4842, 4971
AccII CGCG 3 cut(s) 245, 247, 423
AciI CCGC 9 cut(s) 421, 1921, 2272, 4046, 4200, 4255, 4365, 5278, 5637
AclI AACGTT 1 cut(s) 1754
AcoI YGGCCR 6 cut(s) 961, 1918, 3714, 3721, 4735, 5413
AcsI RAATTY 8 cut(s) 1261, 1269, 1624, 2292, 4639, 4648, 4907, 5268
AcuI CTGAAG 3 cut(s) 1309, 1509, 4068
AcvI CACGTG 2 cut(s) 3307, 4345
AcyI GRCGYC 1 cut(s) 3571
AdeI CACNNNGTG 1 cut(s) 4430
AfaI GTAC 9 cut(s) 1016, 2130, 2137, 2367, 2806, 2823, 3157, 3218, 3953
AfeI AGCGCT 1 cut(s) 3790
AfiI CCNNNNNNNGG 8 cut(s) 272, 497, 1295, 2263, 2981, 4886, 4985, 5411
AflIII ACRYGT 4 cut(s) 789, 2283, 3304, 5143
AhlI ACTAGT 1 cut(s) 5458
AjiI CACGTC 2 cut(s) 4876, 5144
AjnI CCWGG 8 cut(s) 1288, 1566, 2131, 2491, 2868, 3466, 3943, 4849
AjuI GAANNNNNNNTTGG 2 cut(s) 2088, 2120
AleI CACNNNNGTG 1 cut(s) 3533
Alw21I GWGCWC 4 cut(s) 2427, 2726, 3486, 3783
Alw44I GTGCAC 1 cut(s) 2722
AlwNI CAGNNNCTG 2 cut(s) 4667, 4934
Ama87I CYCGRG 2 cut(s) 951, 4161
Aor51HI AGCGCT 1 cut(s) 3790
ApaLI GTGCAC 1 cut(s) 2722
ApoI RAATTY 8 cut(s) 1261, 1269, 1624, 2292, 4639, 4648, 4907, 5268
AseI ATTAAT 2 cut(s) 984, 4194
Asp700I GAANNNNTTC 3 cut(s) 1127, 1198, 2967
AspLEI GCGC 6 cut(s) 245, 247, 425, 3371, 3791, 4603
AspS9I GGNCC 7 cut(s) 80, 1443, 1578, 2016, 2165, 3500, 5195
AsuC2I CCSGG 6 cut(s) 952, 953, 1581, 3591, 4622, 5219
AvaI CYCGRG 2 cut(s) 951, 4161
AvaII GGWCC 3 cut(s) 80, 1443, 2165
AxyI CCTNAGG 2 cut(s) 1425, 3753
BaeGI GKGCMC 3 cut(s) 2491, 2726, 5571
BaeI ACNNNNGTAYC 4 cut(s) 939, 972, 2127, 2160
BalI TGGCCA 4 cut(s) 963, 3723, 4737, 5415
BamHI GGATCC 1 cut(s) 4332
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 2 cut(s) 3783, 4027
BauI CACGAG 2 cut(s) 2952, 3257
BbrPI CACGTG 2 cut(s) 3307, 4345
BbsI GAAGAC 4 cut(s) 1144, 1802, 2216, 2320
Bbv12I GWGCWC 4 cut(s) 2427, 2726, 3486, 3783
BceAI ACGGC 4 cut(s) 809, 2259, 3701, 5604
BcgI CGANNNNNNTGC 4 cut(s) 1482, 1516, 2522, 2556
BciT130I CCWGG 8 cut(s) 1290, 1568, 2133, 2493, 2870, 3468, 3945, 4851
BciVI GTATCC 3 cut(s) 940, 3748, 4982
BclI TGATCA 1 cut(s) 5535
BcnI CCSGG 6 cut(s) 952, 953, 1581, 3591, 4622, 5219
BcuI ACTAGT 1 cut(s) 5458
BfaI CTAG 6 cut(s) 1266, 1761, 2472, 3336, 3963, 5459
BfmI CTRYAG 6 cut(s) 288, 2370, 3784, 4153, 4881, 5725
BfoI RGCGCY 1 cut(s) 3792
BfuAI ACCTGC 2 cut(s) 2377, 5413
BfuI GTATCC 3 cut(s) 940, 3748, 4982
BglII AGATCT 1 cut(s) 4351
BlpI GCTNAGC 1 cut(s) 3398
BmcAI AGTACT 2 cut(s) 2806, 3218
Bme18I GGWCC 3 cut(s) 80, 1443, 2165
BmeT110I CYCGRG 2 cut(s) 951, 4161
BmgBI CACGTC 2 cut(s) 4876, 5144
BmgT120I GGNCC 7 cut(s) 80, 1443, 1578, 2016, 2165, 3500, 5195
BmiI GGNNCC 8 cut(s) 6, 684, 1445, 2017, 2314, 2490, 4026, 4334
BmrI ACTGGG 5 cut(s) 324, 1682, 2653, 3208, 3353
BmuI ACTGGG 5 cut(s) 324, 1682, 2653, 3208, 3353
BoxI GACNNNNGTC 1 cut(s) 4868
BpiI GAAGAC 4 cut(s) 1144, 1802, 2216, 2320
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 5 cut(s) 72, 981, 1858, 2934, 4833
Bpu1102I GCTNAGC 1 cut(s) 3398
BpuEI CTTGAG 4 cut(s) 698, 804, 864, 1340
BpuMI CCSGG 6 cut(s) 952, 953, 1581, 3591, 4622, 5219
Bsa29I ATCGAT 2 cut(s) 257, 5430
BsaAI YACGTR 2 cut(s) 3307, 4345
BsaBI GATNNNNATC 1 cut(s) 5158
BsaHI GRCGYC 1 cut(s) 3571
BsaJI CCNNGG 8 cut(s) 825, 951, 1025, 1439, 2892, 3883, 4756, 5000
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 8 cut(s) 434, 464, 3378, 3408, 3616, 3646, 4907, 4937
Bsc4I CCNNNNNNNGG 8 cut(s) 272, 497, 1295, 2263, 2981, 4886, 4985, 5411
Bse21I CCTNAGG 2 cut(s) 1425, 3753
Bse3DI GCAATG 5 cut(s) 2398, 2713, 2996, 3693, 3831
Bse8I GATNNNNATC 1 cut(s) 5158
BseBI CCWGG 8 cut(s) 1290, 1568, 2133, 2493, 2870, 3468, 3945, 4851
BseCI ATCGAT 2 cut(s) 257, 5430
BseDI CCNNGG 8 cut(s) 825, 951, 1025, 1439, 2892, 3883, 4756, 5000
BseJI GATNNNNATC 1 cut(s) 5158
BseLI CCNNNNNNNGG 8 cut(s) 272, 497, 1295, 2263, 2981, 4886, 4985, 5411
BseMI GCAATG 5 cut(s) 2398, 2713, 2996, 3693, 3831
BseMII CTCAG 8 cut(s) 201, 265, 483, 1158, 1827, 2620, 3744, 4579
BsePI GCGCGC 1 cut(s) 243
BseRI GAGGAG 5 cut(s) 442, 675, 2243, 2581, 3054
BseSI GKGCMC 3 cut(s) 2491, 2726, 5571
BseYI CCCAGC 2 cut(s) 2347, 2682
BsgI GTGCAG 2 cut(s) 1412, 5148
Bsh1236I CGCG 3 cut(s) 245, 247, 423
BshNI GGYRCC 1 cut(s) 2488
BshVI ATCGAT 2 cut(s) 257, 5430
BsiHKAI GWGCWC 4 cut(s) 2427, 2726, 3486, 3783
BsiHKCI CYCGRG 2 cut(s) 951, 4161
BsiSI CCGG 6 cut(s) 131, 952, 1581, 3591, 4622, 5219
BslFI GGGAC 3 cut(s) 2660, 3040, 4862
BslI CCNNNNNNNGG 8 cut(s) 272, 497, 1295, 2263, 2981, 4886, 4985, 5411
BsmBI CGTCTC 5 cut(s) 16, 254, 1163, 1225, 5310
BsmFI GGGAC 3 cut(s) 2660, 3040, 4862
BsmI GAATGC 1 cut(s) 3865
BsoBI CYCGRG 2 cut(s) 951, 4161
Bsp1286I GDGCHC 7 cut(s) 2427, 2491, 2726, 3486, 3783, 4027, 5571
Bsp1407I TGTACA 1 cut(s) 1014
Bsp1720I GCTNAGC 1 cut(s) 3398
Bsp19I CCATGG 2 cut(s) 1439, 5000
BspACI CCGC 9 cut(s) 421, 1921, 2272, 4046, 4200, 4255, 4365, 5278, 5637
BspCNI CTCAG 8 cut(s) 202, 264, 484, 1159, 1828, 2619, 3745, 4578
BspDI ATCGAT 2 cut(s) 257, 5430
BspFNI CGCG 3 cut(s) 245, 247, 423
BspHI TCATGA 2 cut(s) 187, 2260
BspLI GGNNCC 8 cut(s) 6, 684, 1445, 2017, 2314, 2490, 4026, 4334
BspMAI CTGCAG 5 cut(s) 292, 2374, 3788, 4157, 4885
BspMI ACCTGC 2 cut(s) 2377, 5413
BspT107I GGYRCC 1 cut(s) 2488
BsrDI GCAATG 5 cut(s) 2398, 2713, 2996, 3693, 3831
BsrGI TGTACA 1 cut(s) 1014
BssECI CCNNGG 8 cut(s) 825, 951, 1025, 1439, 2892, 3883, 4756, 5000
BssHII GCGCGC 1 cut(s) 243
BssNAI GTATAC 1 cut(s) 3046
BssNI GRCGYC 1 cut(s) 3571
BssSI CACGAG 2 cut(s) 2952, 3257
BssT1I CCWWGG 6 cut(s) 1025, 1439, 2892, 3883, 4756, 5000
Bst1107I GTATAC 1 cut(s) 3046
Bst2BI CACGAG 2 cut(s) 2952, 3257
Bst2UI CCWGG 8 cut(s) 1290, 1568, 2133, 2493, 2870, 3468, 3945, 4851
Bst6I CTCTTC 5 cut(s) 874, 3849, 4316, 5693, 5725
BstACI GRCGYC 1 cut(s) 3571
BstAPI GCANNNNNTGC 4 cut(s) 296, 3004, 4304, 5339
BstAUI TGTACA 1 cut(s) 1014
BstBAI YACGTR 2 cut(s) 3307, 4345
BstC8I GCNNGC 8 cut(s) 245, 961, 2987, 2996, 3865, 3898, 4285, 5340
BstDSI CCRYGG 3 cut(s) 825, 1439, 5000
BstEII GGTNACC 1 cut(s) 3758
BstENI CCTNNNNNAGG 1 cut(s) 4884
BstFNI CGCG 3 cut(s) 245, 247, 423
BstH2I RGCGCY 1 cut(s) 3792
BstHHI GCGC 6 cut(s) 245, 247, 425, 3371, 3791, 4603
BstNI CCWGG 8 cut(s) 1290, 1568, 2133, 2493, 2870, 3468, 3945, 4851
BstNSI RCATGY 4 cut(s) 2287, 3269, 4239, 5342
BstPAI GACNNNNGTC 1 cut(s) 4868
BstPI GGTNACC 1 cut(s) 3758
BstSFI CTRYAG 6 cut(s) 288, 2370, 3784, 4153, 4881, 5725
BstSLI GKGCMC 3 cut(s) 2491, 2726, 5571
BstUI CGCG 3 cut(s) 245, 247, 423
BstV2I GAAGAC 4 cut(s) 1144, 1802, 2216, 2320
BstX2I RGATCY 7 cut(s) 829, 1042, 2233, 4332, 4351, 5008, 5153
BstXI CCANNNNNNTGG 2 cut(s) 2309, 3850
BstYI RGATCY 7 cut(s) 829, 1042, 2233, 4332, 4351, 5008, 5153
BstZ17I GTATAC 1 cut(s) 3046
Bsu15I ATCGAT 2 cut(s) 257, 5430
Bsu36I CCTNAGG 2 cut(s) 1425, 3753
BsuI GTATCC 3 cut(s) 940, 3748, 4982
BsuTUI ATCGAT 2 cut(s) 257, 5430
BtgI CCRYGG 3 cut(s) 825, 1439, 5000
BtgZI GCGATG 1 cut(s) 48
BtrI CACGTC 2 cut(s) 4876, 5144
BtsI GCAGTG 2 cut(s) 1223, 5272
BveI ACCTGC 2 cut(s) 2377, 5413
Cac8I GCNNGC 8 cut(s) 245, 961, 2987, 2996, 3865, 3898, 4285, 5340
CaiI CAGNNNCTG 2 cut(s) 4667, 4934
CciI TCATGA 2 cut(s) 187, 2260
CfoI GCGC 6 cut(s) 245, 247, 425, 3371, 3791, 4603
Cfr13I GGNCC 7 cut(s) 80, 1443, 1578, 2016, 2165, 3500, 5195
Cfr9I CCCGGG 1 cut(s) 951
ClaI ATCGAT 2 cut(s) 257, 5430
CseI GACGC 3 cut(s) 236, 3579, 3966
CsiI ACCWGGT 2 cut(s) 2131, 2868
Csp6I GTAC 9 cut(s) 1015, 2129, 2136, 2366, 2805, 2822, 3156, 3217, 3952
CviQI GTAC 9 cut(s) 1015, 2129, 2136, 2366, 2805, 2822, 3156, 3217, 3952
DraI TTTAAA 3 cut(s) 1743, 3325, 4780
DraIII CACNNNGTG 1 cut(s) 4430
EaeI YGGCCR 6 cut(s) 961, 1918, 3714, 3721, 4735, 5413
Eam1104I CTCTTC 5 cut(s) 874, 3849, 4316, 5693, 5725
EarI CTCTTC 5 cut(s) 874, 3849, 4316, 5693, 5725
EciI GGCGGA 1 cut(s) 4035
Ecl136II GAGCTC 1 cut(s) 3781
Eco130I CCWWGG 6 cut(s) 1025, 1439, 2892, 3883, 4756, 5000
Eco24I GRGCYC 2 cut(s) 3783, 4027
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 3 cut(s) 80, 1443, 2165
Eco47III AGCGCT 1 cut(s) 3790
Eco53kI GAGCTC 1 cut(s) 3781
Eco57I CTGAAG 3 cut(s) 1309, 1509, 4068
Eco72I CACGTG 2 cut(s) 3307, 4345
Eco81I CCTNAGG 2 cut(s) 1425, 3753
Eco88I CYCGRG 2 cut(s) 951, 4161
Eco91I GGTNACC 1 cut(s) 3758
EcoICRI GAGCTC 1 cut(s) 3781
EcoNI CCTNNNNNAGG 1 cut(s) 4884
EcoO109I RGGNCCY 1 cut(s) 2016
EcoO65I GGTNACC 1 cut(s) 3758
EcoRII CCWGG 8 cut(s) 1288, 1566, 2131, 2491, 2868, 3466, 3943, 4849
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 6 cut(s) 1025, 1439, 2892, 3883, 4756, 5000
EcoT22I ATGCAT 1 cut(s) 1083
EcoT38I GRGCYC 2 cut(s) 3783, 4027
ErhI CCWWGG 6 cut(s) 1025, 1439, 2892, 3883, 4756, 5000
Esp3I CGTCTC 5 cut(s) 16, 254, 1163, 1225, 5310
FalI AAGNNNNNCTT 4 cut(s) 2885, 2917, 3657, 3689
FaqI GGGAC 3 cut(s) 2660, 3040, 4862
FauI CCCGC 1 cut(s) 4193
FauNDI CATATG 2 cut(s) 2140, 3970
FbaI TGATCA 1 cut(s) 5535
FblI GTMKAC 6 cut(s) 466, 1607, 3045, 4134, 4842, 4971
FriOI GRGCYC 2 cut(s) 3783, 4027
FspBI CTAG 6 cut(s) 1266, 1761, 2472, 3336, 3963, 5459
GlaI GCGC 6 cut(s) 244, 246, 424, 3370, 3790, 4602
GsaI CCCAGC 2 cut(s) 2351, 2686
GsuI CTGGAG 5 cut(s) 72, 981, 1858, 2934, 4833
HaeII RGCGCY 1 cut(s) 3792
HapII CCGG 6 cut(s) 131, 952, 1581, 3591, 4622, 5219
HgaI GACGC 3 cut(s) 236, 3579, 3966
HhaI GCGC 6 cut(s) 245, 247, 425, 3371, 3791, 4603
Hin1I GRCGYC 1 cut(s) 3571
Hin6I GCGC 6 cut(s) 243, 245, 423, 3369, 3789, 4601
HinP1I GCGC 6 cut(s) 243, 245, 423, 3369, 3789, 4601
HincII GTYRAC 9 cut(s) 100, 549, 1608, 2146, 2251, 2269, 2446, 3523, 4843
HindII GTYRAC 9 cut(s) 100, 549, 1608, 2146, 2251, 2269, 2446, 3523, 4843
HindIII AAGCTT 7 cut(s) 386, 593, 916, 2306, 2382, 2450, 2577
HpaI GTTAAC 3 cut(s) 100, 2251, 2446
HpaII CCGG 6 cut(s) 131, 952, 1581, 3591, 4622, 5219
Hpy99I CGWCG 1 cut(s) 5145
Hsp92I GRCGYC 1 cut(s) 3571
HspAI GCGC 6 cut(s) 243, 245, 423, 3369, 3789, 4601
Ksp22I TGATCA 1 cut(s) 5535
KspAI GTTAAC 3 cut(s) 100, 2251, 2446
LmnI GCTCC 8 cut(s) 688, 773, 1468, 2318, 2919, 3262, 4030, 5576
MabI ACCWGGT 2 cut(s) 2131, 2868
MaeI CTAG 6 cut(s) 1266, 1761, 2472, 3336, 3963, 5459
MfeI CAATTG 2 cut(s) 3651, 3915
MflI RGATCY 7 cut(s) 829, 1042, 2233, 4332, 4351, 5008, 5153
MhlI GDGCHC 7 cut(s) 2427, 2491, 2726, 3486, 3783, 4027, 5571
MlsI TGGCCA 4 cut(s) 963, 3723, 4737, 5415
MluNI TGGCCA 4 cut(s) 963, 3723, 4737, 5415
MlyI GAGTC 7 cut(s) 426, 1349, 1817, 1865, 2859, 4838, 5462
MmeI TCCRAC 2 cut(s) 1684, 1830
Mox20I TGGCCA 4 cut(s) 963, 3723, 4737, 5415
Mph1103I ATGCAT 1 cut(s) 1083
MroXI GAANNNNTTC 3 cut(s) 1127, 1198, 2967
MscI TGGCCA 4 cut(s) 963, 3723, 4737, 5415
MslI CAYNNNNRTG 8 cut(s) 1086, 2064, 3236, 3533, 3848, 4104, 4464, 5543
Msp20I TGGCCA 4 cut(s) 963, 3723, 4737, 5415
MspA1I CMGCKG 1 cut(s) 3896
MspI CCGG 6 cut(s) 131, 952, 1581, 3591, 4622, 5219
MunI CAATTG 2 cut(s) 3651, 3915
Mva1269I GAATGC 1 cut(s) 3865
MvaI CCWGG 8 cut(s) 1290, 1568, 2133, 2493, 2870, 3468, 3945, 4851
MvnI CGCG 3 cut(s) 245, 247, 423
NciI CCSGG 6 cut(s) 952, 953, 1581, 3591, 4622, 5219
NcoI CCATGG 2 cut(s) 1439, 5000
NdeI CATATG 2 cut(s) 2140, 3970
NlaIV GGNNCC 8 cut(s) 6, 684, 1445, 2017, 2314, 2490, 4026, 4334
NsiI ATGCAT 1 cut(s) 1083
NspI RCATGY 4 cut(s) 2287, 3269, 4239, 5342
OliI CACNNNNGTG 1 cut(s) 3533
PaeI GCATGC 1 cut(s) 5342
PagI TCATGA 2 cut(s) 187, 2260
PauI GCGCGC 1 cut(s) 243
PciI ACATGT 1 cut(s) 2283
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PctI GAATGC 1 cut(s) 3865
PdmI GAANNNNTTC 3 cut(s) 1127, 1198, 2967
PflFI GACNNNGTC 2 cut(s) 2674, 4113
PfoI TCCNGGA 1 cut(s) 4849
PleI GAGTC 7 cut(s) 425, 1348, 1816, 1865, 2858, 4838, 5461
PmaCI CACGTG 2 cut(s) 3307, 4345
PmlI CACGTG 2 cut(s) 3307, 4345
PpsI GAGTC 7 cut(s) 425, 1348, 1816, 1865, 2858, 4838, 5461
Ppu21I YACGTR 2 cut(s) 3307, 4345
PscI ACATGT 1 cut(s) 2283
PshAI GACNNNNGTC 1 cut(s) 4868
PshBI ATTAAT 2 cut(s) 984, 4194
PsiI TTATAA 1 cut(s) 2658
Psp124BI GAGCTC 1 cut(s) 3783
Psp1406I AACGTT 1 cut(s) 1754
Psp6I CCWGG 8 cut(s) 1288, 1566, 2131, 2491, 2868, 3466, 3943, 4849
PspCI CACGTG 2 cut(s) 3307, 4345
PspEI GGTNACC 1 cut(s) 3758
PspFI CCCAGC 2 cut(s) 2347, 2682
PspGI CCWGG 8 cut(s) 1288, 1566, 2131, 2491, 2868, 3466, 3943, 4849
PspN4I GGNNCC 8 cut(s) 6, 684, 1445, 2017, 2314, 2490, 4026, 4334
PspPI GGNCC 7 cut(s) 80, 1443, 1578, 2016, 2165, 3500, 5195
PstI CTGCAG 5 cut(s) 292, 2374, 3788, 4157, 4885
PstNI CAGNNNCTG 2 cut(s) 4667, 4934
PsuI RGATCY 7 cut(s) 829, 1042, 2233, 4332, 4351, 5008, 5153
PsyI GACNNNGTC 2 cut(s) 2674, 4113
PteI GCGCGC 1 cut(s) 243
PvuII CAGCTG 1 cut(s) 3896
RsaI GTAC 9 cut(s) 1016, 2130, 2137, 2367, 2806, 2823, 3157, 3218, 3953
RsaNI GTAC 9 cut(s) 1015, 2129, 2136, 2366, 2805, 2822, 3156, 3217, 3952
RseI CAYNNNNRTG 8 cut(s) 1086, 2064, 3236, 3533, 3848, 4104, 4464, 5543
SacI GAGCTC 1 cut(s) 3783
SalI GTCGAC 2 cut(s) 1606, 4841
Sau96I GGNCC 7 cut(s) 80, 1443, 1578, 2016, 2165, 3500, 5195
ScaI AGTACT 2 cut(s) 2806, 3218
SchI GAGTC 7 cut(s) 426, 1349, 1817, 1865, 2859, 4838, 5462
SduI GDGCHC 7 cut(s) 2427, 2491, 2726, 3486, 3783, 4027, 5571
SexAI ACCWGGT 2 cut(s) 2131, 2868
SfcI CTRYAG 6 cut(s) 288, 2370, 3784, 4153, 4881, 5725
SinI GGWCC 3 cut(s) 80, 1443, 2165
SmaI CCCGGG 1 cut(s) 953
SmiMI CAYNNNNRTG 8 cut(s) 1086, 2064, 3236, 3533, 3848, 4104, 4464, 5543
SmlI CTYRAG 4 cut(s) 677, 819, 879, 1355
SmoI CTYRAG 4 cut(s) 677, 819, 879, 1355
SpeI ACTAGT 1 cut(s) 5458
SphI GCATGC 1 cut(s) 5342
SsiI CCGC 9 cut(s) 421, 1921, 2272, 4046, 4200, 4255, 4365, 5278, 5637
SspI AATATT 1 cut(s) 3456
SspMI CTAG 6 cut(s) 1266, 1761, 2472, 3336, 3963, 5459
SstI GAGCTC 1 cut(s) 3783
StyI CCWWGG 6 cut(s) 1025, 1439, 2892, 3883, 4756, 5000
TatI WGTACW 5 cut(s) 1014, 2804, 3155, 3216, 3951
TauI GCSGC 3 cut(s) 1923, 5280, 5640
TspGWI ACGGA 7 cut(s) 255, 373, 534, 1187, 4708, 4968, 5104
TspMI CCCGGG 1 cut(s) 951
Tth111I GACNNNGTC 2 cut(s) 2674, 4113
VneI GTGCAC 1 cut(s) 2722
VpaK11BI GGWCC 3 cut(s) 80, 1443, 2165
VspI ATTAAT 2 cut(s) 984, 4194
XagI CCTNNNNNAGG 1 cut(s) 4884
XapI RAATTY 8 cut(s) 1261, 1269, 1624, 2292, 4639, 4648, 4907, 5268
XbaI TCTAGA 3 cut(s) 1265, 1760, 3962
XceI RCATGY 4 cut(s) 2287, 3269, 4239, 5342
XcmI CCANNNNNNNNNTGG 2 cut(s) 849, 1597
XmaI CCCGGG 1 cut(s) 951
XmiI GTMKAC 6 cut(s) 466, 1607, 3045, 4134, 4842, 4971
XmnI GAANNNNTTC 3 cut(s) 1127, 1198, 2967
XspI CTAG 6 cut(s) 1266, 1761, 2472, 3336, 3963, 5459
ZrmI AGTACT 2 cut(s) 2806, 3218
Zsp2I ATGCAT 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.