MD17G1026700.v1.1

Bacterial Ig-like domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014163
Physical Location & Seq
Reverse (-)
1907720 .. 1919647
11928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1026700.v1.1.491

Sequence Viewer

Length: 5913 bp
ATGAAGATGCTGAGGTACACTGTTTTGCTCGGTTTGTTTCTTCTGCTGGTGGCGGGCCAAACGGTGTCGCATTTGGGCTCCGGTCCGCACATTGCAGACGTCAACATTCTGCTGCCTCCCAGAATGACTCATCCGGTCGAGTATCGGCTCCAGGGGAGCGATGGTTGCTTCAAATGGTCATGGGATCACCACGACATTCTGTCTGTTCTACCAGAATACAATACAACTAGTCACTGCTCGACGAGTGCGCTGTTGAGATCAATTGCTCCGTTTAGTGGCCGGAAGGAAACTGCTGTTTATGCTGCTGATGTAAATACTGGAGTTGTGATTCGCTGCAAAGTTTTTATTGACAAGCTTTCTAGGATTCAGATATTTCATAATTCTGTTAAACTTGACTTGGATGGGCTTGCCACGCTTCGAGTTCGCGCCTTTGACAGCGAAGAAAATGTGTTTTCGTCACTGGTGGGATTGCAATTTATGTGGCAGCTGGAAACCAATGAACCGCCTCACCACCTTGCTCATGTTCCTCTAAAGGACTCTCCACTGAGTGACTGTGGTGGGTTGTGTGGAGACCTAGATATCCAAATAAGCCTTGAAGATAATGGTGTATTTTCTGATCTGTACGTTGTAAAGGGAATCGAAATTGGGCATGAGATTGTTTCTGTACATTTGCTTGAGCCACGATTCCAGCACATGACTGATAGAATTGCTCTTACTGTAGCAGAAGCCATGTCACTTGATCCTCCCTCACCTGTTTTTGTCCTCGTTGGTGCTGCTGTTCAGTATAGTCTAAAAATTATCCGTGGGAACAAAGCTCAAGTGGTAACTTTGCCGTCTCCACATCATCGATGGTCTGTTTCAAACTCTTCCGTTGCTCGTGTGGACTCTATGATGGGTTTGGCTAATGCGTTTAGCTTAGGAATTACAAACACCATAGTTGAAGATACAAGGGTTGCTGGCCACATACAAGTGTCGTCACTTAATGTTGTCCTGCCAGATTCTTTATCTTTATACATTGTACCTTTGTCCACTTCTGGTGATCTTGTTGAGGGAATTGAAGCAATTCCATCCATGACACGTTGGTATGGTGTTTCTGGCCGTCAATACCTCATTCAAATGAAGGTTTTCTCACAGGGTCCAGATGCACAAGAAATCTATCTTACAGAGAGTGATGATTTAAAGTTGTCCAGTGACCAGTCTGACTACTGGAGACTTTCTCCTGTATCAAACGATATTGCAATCAAACATGGCTGGCAGAATTCTATGATTCTGAAGGCAACTTCACAGGGACAGGGTAAATTGGCAGCTTCTTTGACTTACTTTAGTGCATTAAATGAGAAGAAGGAGGTTCTCAAGGTCATGCAAGAAGTCGTGGTTTGTGATCGAGTTCAGTTTACTTTGGATAAAAGTGGTGCATCTCCAACCATTCTCCTTCCGTGGGCCCCTTCTGTTTATCAGGAGGTGGAGCTAAAGGCTACAGGAGGTTGTGCTAAAGCATCCAGTGACTACAAATGGTTCTCTTCAGACATGGGTATTGTGTCTGTATCTGCTTCTGGTGTTGTCCAGGCTAAGAAGCCTGGTAAAGCTACCATTAAAGTGCTATCCATTTTTGATTCATTTAATTATGATGAGGTTGCCATTGAAGTCTCTGTTCCAGCATCTATGGTCATGCTCCGTAACTTCCCTGTTGAGACTGTTGTAGGAACACATCTTCAAGCTGCTGTAACAATGAAAGCATCAAATGGTGCCTACTTTTATAGATGTGATGCATTCAGTTCCTTCATAAAGTGGAAAGCAGGGAGCGAGTCTTTCATTATTGTCAATGCAACAGTTGAGGCAACTGCTTTAGACATTTTAGGGAATGCTGAGTTCTATGCATCAAGTTCTGGTCCTGCATGTTCACGGGCATATTTATATGCCTCGGCTTCTGGTCGGGCAACCCTTCATGCTACATTATCAAAAGAATATCACAACTTGGATAGCTCTATTAGCGGGCCTGTTGTTTTAAAAGCATCTTCACTTATTGCGGCATATTCACCGCTTAGCATTCGTCAGGCAGGTGATGGAAACCATTTTGGTGGCTACTTTTTTGATTTGGCTCAAACAGAAACTGATAGGCAGTTGGTAAATTTGGACAAAATATACCTTGTCCCTGGAACGCATCTGGATGTTATGCTTCTTGGTGGACCTGAAAAGTGGAACAATGGTATTGACTTTGTTGAAACTATGGATATCTTAAATGAAGGGCATGGTCATATTGACAATGGGGCTTCTGTGCAACGGTTATCTGATAGCTATAAGAGTCTGTACAGAGTTTCATGTCAAATGCTGGGAACCTATAAAATTGTTTTCAAACGTGGTAATATGGTTGGGGATGGCCATCCTCTACCTGCAGTAGCAGAAGTTCCGCTGTATCTTACATGTAGCGTTCCTACCTCAATTGTTCTGCTAGCTGATGAACATGTAAATGAACGTGAAGTTATACGGACTGCAATTCAGGCTGATCGCAGTTCTGGGAAAATCCGTGTCACCCCTGTTACTGTGGCAAATGGGCGCACCATTAGATTAGCTGCTGTTGGTATTAGTAACTCTGGAGAAGCGTTTGCAAACTCATCTTCTCTTTCCTTGAGGTGGGAACTTATCAATTGTGGCGAGATGGCTAGTTGGGATGATGCTGATGATTTAGAGAGGTCAGAGCACAGTTGGGAGAGGTTATTGAGCTTGAAAAATGAGTCAGGCCTGTGTATAGTTCGTGCTACTGTCATTGGTTTCAGAGATAACATGGGTGCTCATAAGTCTGTTCCACTGCTTGAGGATTCAGATAATGTTCTTGCAGATGCAATTCGTCTACAGCTTGTTTCTACACTAATTGTCAGCCCAGAGTTCAATCTGGTGTATTTCAATCCCAATGCTAAGTTAAACCTGTCAATTACTGGTGGGAGCTGTTTCTTGGAAGCTACTGTGAACAATTCTCGAGTACTGGAAGTCGTTCAACCCCCAACAGGTTTACAGTGCTCACAACTGATCCTGTCTCCTAAAGCTATGGGGACGGCACTTGTGACAGTTTACGATATTGGACTTGCTCCTCCCCTTGCAGCTTCTGCTGTGGTACAAGTTGTAGACATTGACTGGATAAAGATTGTGTCACCAGACGAAATAAGCCTTATGGAAGGGAACTCGCAGACTATTGATTTGATGGCTGGGATTAGTGATGGGAGGACTTTCGATGCTTATCAGTTTGCCTACATGAATATTCATGTGCATGTTGAGGATCATATTATTGAGGTGTTAGACATTAATGATATCTCAAGCCCAGGTGGTGGATTTGTTAATGTCCCGAAATTTAAGATTTTGGCTACACATCTTGGGATCACCACTTTCTTTGTGAGTGCTGTACAACAATCTGGGCACGAAATTTTGAGTCAACCAATAATGGTGGAAGTCTATGCACCACCAGTAATCTACCCACAAGAAATTTTCCTTGTACCTGGAGCATCTTATGTGCTTACCGTGAAAGGAGGACCAACAGTTGGTGTATATGTTGAATATATGAGTCTGGATGATGAAATAGTGGCAATGCATAGATCTTCTGGACGGCTGTCTGCCATGTCACCTGGAAACACTACTATTCGTGCCACATTCTTCAGAAATGGAGATATTGTGATTTGTGAAGCATATGGCAGTGTTAAAGTAGGAGTTCCTTCTTCGGTGATACTGAATGCACAAAGTGAACTGCTTGGTGTTGGCCATGAGATGCCAATATATCCTTTATTTTCTCAGGGTGATTTGTTTTCTGTTTATGAGCTTTGCCAAAACTACCGGTGGACTATAGAAGATGAAAAGGTTTTGAGCTTTTATTTAGAGCACTTCACTGGTGAGAAGTATGGTTCTCAGTTGGAACCTTCAGAAAAGATTCAATTCCCTAGCCATATGAGTGAGGAAGAGCTTGGTTTTATCAAAGTCATATCTGGAAGATTTGCAGGGAGAACAAACGTTGCAGTCTCGTTCTCATGTGAATTTATATCTTCTGGTTCCAAATCATGGAGAAGAGTTTACAATGCATCTGTATCAATATCGGTGGTGCCTGATCTCCCTCTTGCTCTTGGAGTCCCAATAACCTGGGTTCTTCCTCCTCATTATACTACAACAAGTATTTTGCCTTCATCTTCAGAATTGCATGGCCAAAGGGATAGTCAGAGTCATAAAGGAACTATTATCTATTCCTTATTAAAAAATCTCCCTGAAAAGAATGACGGTGTGCAAAAGGATGCTATTTCCATCGATGGAGACAGAATCAAGACATCAGAGAGTAACAATCTTGCATGCATTCAGGCAAAAGATCGTATGACGGGTAGAAGTGAGATTGCTGCTTGCGTCAAAGTTGCTGAGGTGTCTCAAATAAGAATTACGAACAAGGAGTTACCATTTCATGGGATCAATCTTGCTGTTGGTGCTGAAATTTCTCTTCCAGTAGTCTACCTTGATGTGCTAGGAAACCCTTTCTATGAAGCACACGGTGCCGTTCTCTTTGATATGGCAACCAACTCTCCTGATGTTGTGTCCATAAACAACAGCAGCAACACGCATGTTGGCAGTGGAAATATCCATCTCAAGGCGATGCGACATGGTAGAGCGCTTGTGCGAATATCCATCCATCGTATGCCTCAGAAGTCGGACTATATCCTGGTATCAGTGGGTGCTCATATACATCCTCAAAACCCAGTTCTTCGAACAGGAAGCCATCTTAACTTCAGCATTGAAGGCTTAAATGATGAAATTTCTGGCCGTTGGAGAACTGCAAATGGAAGTGTCATATCTGTATCCCCATTGTCTGGGGTGGCTGAAGTAATTGGGGAAGGTACAACTCAAGTATATTTTGAAGCTTCAAGTTTGAAGCTGCGAACAACAGTTATAGTGCCAACAGAGGATATTGTTTCCGTGGATGCTCCAACTGAGACACTAACAAATGTTCCTTTCCCAACTAAAGGATATAACTTCTATGTGAAGATCAGTACTGACAACGAGTTTAAGGCGCTTGGAAACACCAAGGAACTCCAGTATGACTGTAGAGTTGACCCGCCTTTTGTTGGGTACGCAAAACCATGGTTAGATCTTGATACCGGTAGCTCATACTGCCGCTTTTTTCCTTACTCACCGGAGCATTTGGTTCGTTTGGTACCCAAGTCAAAGGATATGAAATCAGATATATCTGTTTCCATCAAAGCTTCACTGAGAAAAGCAGATCATGTTTCCGGATCTGCTTCTGCTCTTTTTGTTGGAGGGTTTTCCATTTTAGAGATGGGCAAGGATTCAATGCAGTTGAAGCTGACCCCAGACTCTAACAAGACTACCATAACAATCCTGGGAAACACAGATGTTGAAATCTATAGGCATGAGCGGGATTCTTTACACATTACTCCCATCCATAAAGAAGGTTCTGGGATCGGTGGGCGTGCAAAATATGAGGTCAAAATGCTAGGATCCAAGAGATTCAAAGATACAATTTTCATTACACTTCCAGCCAATGGACAGAGTGTGGAAATTGATGTCAACGGTGACCCTGGAGATAAAACGCCATCTGAAACTACCTTCGATCGTCCACTTTGGCTAATGGTGATTGCATATATTGCAATCGTGCTTGGATGTCTTGTTATATTGGGTTGGATGGTGGCTTGCATCATATGTTATTGGGCGGACGAACTGGATAGATCTCGAAGATCCATTAACGCTCCAGCTACTCCAAGCACTGCAGGCCCAGCCACACCCGAACGTACTAGCAGTCCAGCTGCTGCGAGCGAACAGTCTCCTCGAACACCTCAGCCGTTCATAGATTACGTTAGAAGAACAATAGACGAAACTCCATATTACAGGCGAGAGCCGCGGAGGAGAGTTAATCCACAGAATACGTTCTAG

Protein Analysis

1971

Amino Acids

215.34

Weight (kDa)

5.75

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_NUP210_1st PF22967 35 - 117 1.8e-16 NUP210 Ig-like domain 1
Ig_NUP210_2nd PF22969 137 - 232 6.3e-10 NUP210 Ig-like domain 2
Ig_NUP210_3rd PF22963 244 - 319 1.8e-06 NUP210 Ig-like domain 3
Ig_NUP210_5th PF26182 475 - 549 7.1e-07 NUP210 Ig-like domain 5
Big_2 PF02368 487 - 535 3.4e-06 Bacterial Ig-like domain (group 2)
Ig_NUP210_7th PF22962 710 - 809 4.4e-15 NUP210 Ig-like domain 7
Ig_GP210_15th PF24425 1642 - 1759 2.5e-50 Nuclear pore complex protein GP210, Ig-like domain
Ig_GP210_16th PF24427 1771 - 1851 7.7e-35 Nuclear pore complex protein GP210, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10590 AT5G40480
fragaria_vesca FvH4_6g51430
malus_domestica MD17G1026700.v1.1
prunus_persica Prupe.3G292000_v2.0.a1 Prupe.3G292000_v2.0.a1
pyrus_communis pycom17g02180
rosa_chinensis RchiOBHm_Chr2g0173101
rosa_laevigata RLG00000022158
rosa_multiflora Rmu_sc0011236.1_g000011
rosa_roxburghii Rroxscaffold_2G00079240
rosa_rugosa Rorug02G0565200
rosa_samantha Rh2AG645300 Rh2BG656000 Rh2CG621100 Rh2DG669400
rosa_wichuraiana Rw2G053000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2048
AatII GACGTC 1 cut(s) 102
Acc36I ACCTGC 2 cut(s) 2048, 2398
Acc65I GGTACC 1 cut(s) 5176
AccB1I GGYRCC 4 cut(s) 1745, 4081, 4517, 5176
AccB7I CCANNNNNTGG 6 cut(s) 3320, 3530, 4041, 4430, 4832, 5525
AccBSI CCGCTC 1 cut(s) 5398
AccI GTMKAC 3 cut(s) 2848, 3120, 4476
AccII CGCG 2 cut(s) 426, 5881
AccIII TCCGGA 1 cut(s) 5252
AclI AACGTT 1 cut(s) 3993
AcoI YGGCCR 7 cut(s) 277, 958, 1096, 2377, 3745, 4180, 4783
AcsI RAATTY 8 cut(s) 1258, 2128, 3341, 3414, 3474, 4016, 4458, 4776
AcuI CTGAAG 7 cut(s) 1292, 1506, 3628, 3888, 4152, 4735, 4863
AcyI GRCGYC 1 cut(s) 99
AdeI CACNNNGTG 3 cut(s) 548, 3728, 4517
AfeI AGCGCT 1 cut(s) 4635
AflIII ACRYGT 3 cut(s) 1076, 2420, 2461
AgeI ACCGGT 2 cut(s) 3819, 5120
AhdI GACNNNNNGTC 1 cut(s) 199
AhlI ACTAGT 1 cut(s) 227
Alw21I GWGCWC 5 cut(s) 2700, 2791, 3017, 3867, 4702
AlwNI CAGNNNCTG 3 cut(s) 2111, 3101, 5789
Ama87I CYCGRG 1 cut(s) 2973
Aor13HI TCCGGA 1 cut(s) 5252
Aor51HI AGCGCT 1 cut(s) 4635
ApaI GGGCCC 1 cut(s) 1444
ApoI RAATTY 8 cut(s) 1258, 2128, 3341, 3414, 3474, 4016, 4458, 4776
ArsI GACNNNNNNTTYG 6 cut(s) 3175, 3207, 4177, 4209, 5066, 5098
AseI ATTAAT 1 cut(s) 3297
AsiGI ACCGGT 2 cut(s) 3819, 5120
Asp700I GAANNNNTTC 5 cut(s) 1062, 1124, 2988, 3912, 5906
Asp718I GGTACC 1 cut(s) 5176
AspLEI GCGC 5 cut(s) 250, 428, 2556, 4636, 5035
AsuII TTCGAA 1 cut(s) 4729
AsuNHI GCTAGC 1 cut(s) 2449
AvaI CYCGRG 1 cut(s) 2973
AvaII GGWCC 5 cut(s) 83, 1136, 1889, 2186, 3521
BaeGI GKGCMC 2 cut(s) 1444, 3411
BaeI ACNNNNGTAYC 4 cut(s) 936, 969, 5168, 5201
BalI TGGCCA 4 cut(s) 960, 2379, 3747, 4182
BamHI GGATCC 1 cut(s) 5480
BanI GGYRCC 4 cut(s) 1745, 4081, 4517, 5176
BanII GRGCYC 2 cut(s) 80, 1444
BarI GAAGNNNNNNTAC 2 cut(s) 4144, 4176
BauI CACGAG 1 cut(s) 876
Bbv12I GWGCWC 5 cut(s) 2700, 2791, 3017, 3867, 4702
BbvCI CCTCAGC 3 cut(s) 11, 4386, 5817
BceAI ACGGC 7 cut(s) 817, 1083, 3066, 3611, 4505, 4770, 5806
BcgI CGANNNNNNTGC 2 cut(s) 5150, 5184
BciVI GTATCC 1 cut(s) 4831
BcuI ACTAGT 1 cut(s) 227
BfmI CTRYAG 8 cut(s) 717, 1476, 2391, 2849, 3828, 5065, 5386, 5748
BfoI RGCGCY 2 cut(s) 4637, 5036
BfuAI ACCTGC 2 cut(s) 2048, 2398
BfuI GTATCC 1 cut(s) 4831
BglII AGATCT 3 cut(s) 3584, 5110, 5708
BlpI GCTNAGC 1 cut(s) 2042
BmcAI AGTACT 2 cut(s) 2979, 5014
Bme18I GGWCC 5 cut(s) 83, 1136, 1889, 2186, 3521
BmeRI GACNNNNNGTC 1 cut(s) 199
BmeT110I CYCGRG 1 cut(s) 2973
BmrI ACTGGG 1 cut(s) 4715
BmtI GCTAGC 1 cut(s) 2453
BmuI ACTGGG 1 cut(s) 4715
BoxI GACNNNNGTC 1 cut(s) 3598
BplI GAGNNNNNCTC 2 cut(s) 1201, 1233
BpmI CTGGAG 8 cut(s) 134, 339, 1228, 2613, 3510, 5039, 5583, 5715
Bpu10I CCTNAGC 4 cut(s) 11, 916, 4386, 5817
Bpu1102I GCTNAGC 1 cut(s) 2042
Bpu14I TTCGAA 1 cut(s) 4729
BpuEI CTTGAG 8 cut(s) 695, 801, 1337, 2647, 2831, 3292, 4595, 4851
Bsa29I ATCGAT 2 cut(s) 847, 4281
BsaBI GATNNNNATC 2 cut(s) 2379, 3231
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 564
BsaWI WCCGGW 6 cut(s) 80, 133, 3819, 5120, 5155, 5252
BsaXI ACNNNNNCTCC 6 cut(s) 148, 178, 3645, 3675, 4531, 4561
Bse118I RCCGGY 2 cut(s) 3819, 5120
Bse3DI GCAATG 2 cut(s) 90, 3582
Bse8I GATNNNNATC 2 cut(s) 2379, 3231
BseAI TCCGGA 1 cut(s) 5252
BseCI ATCGAT 2 cut(s) 847, 4281
BseJI GATNNNNATC 2 cut(s) 2379, 3231
BseMI GCAATG 2 cut(s) 90, 3582
BseMII CTCAG 9 cut(s) 536, 1857, 3791, 3905, 4377, 4679, 4944, 5222, 5831
BseRI GAGGAG 4 cut(s) 3075, 4122, 5796, 5899
BseSI GKGCMC 2 cut(s) 1444, 3411
BseYI CCCAGC 3 cut(s) 2329, 3200, 5755
Bsh1236I CGCG 2 cut(s) 426, 5881
Bsh1285I CGRYCG 2 cut(s) 138, 5596
BshNI GGYRCC 4 cut(s) 1745, 4081, 4517, 5176
BshTI ACCGGT 2 cut(s) 3819, 5120
BshVI ATCGAT 2 cut(s) 847, 4281
BsiEI CGRYCG 2 cut(s) 138, 5596
BsiHKAI GWGCWC 5 cut(s) 2700, 2791, 3017, 3867, 4702
BsiHKCI CYCGRG 1 cut(s) 2973
BsiSI CCGG 7 cut(s) 81, 134, 280, 3820, 5121, 5156, 5253
BslFI GGGAC 5 cut(s) 1302, 2135, 3061, 3320, 4094
BsmBI CGTCTC 1 cut(s) 840
BsmFI GGGAC 5 cut(s) 1302, 2135, 3061, 3320, 4094
BsmI GAATGC 5 cut(s) 1769, 1867, 2046, 3724, 4326
Bso31I GGTCTC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 2973
Bsp119I TTCGAA 1 cut(s) 4729
Bsp120I GGGCCC 1 cut(s) 1440
Bsp1286I GDGCHC 8 cut(s) 80, 1444, 2700, 2791, 3017, 3411, 3867, 4702
Bsp13I TCCGGA 1 cut(s) 5252
Bsp1407I TGTACA 3 cut(s) 664, 2307, 3394
Bsp1720I GCTNAGC 1 cut(s) 2042
Bsp19I CCATGG 1 cut(s) 5102
BspCNI CTCAG 9 cut(s) 537, 1858, 3790, 3904, 4378, 4678, 4945, 5223, 5830
BspDI ATCGAT 2 cut(s) 847, 4281
BspEI TCCGGA 1 cut(s) 5252
BspFNI CGCG 2 cut(s) 426, 5881
BspMAI CTGCAG 2 cut(s) 2395, 5752
BspMI ACCTGC 2 cut(s) 2048, 2398
BspOI GCTAGC 1 cut(s) 2453
BspQI GCTCTTC 1 cut(s) 3936
BspT104I TTCGAA 1 cut(s) 4729
BspT107I GGYRCC 4 cut(s) 1745, 4081, 4517, 5176
BspTNI GGTCTC 1 cut(s) 564
BsrBI CCGCTC 1 cut(s) 5398
BsrDI GCAATG 2 cut(s) 90, 3582
BsrFI RCCGGY 2 cut(s) 3819, 5120
BsrGI TGTACA 3 cut(s) 664, 2307, 3394
BssAI RCCGGY 2 cut(s) 3819, 5120
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 876
BssT1I CCWWGG 2 cut(s) 5046, 5102
Bst2BI CACGAG 1 cut(s) 876
Bst6I CTCTTC 5 cut(s) 871, 1525, 3936, 4042, 4470
BstACI GRCGYC 1 cut(s) 99
BstAPI GCANNNNNTGC 3 cut(s) 3101, 4517, 5627
BstAUI TGTACA 3 cut(s) 664, 2307, 3394
BstBI TTCGAA 1 cut(s) 4729
BstDSI CCRYGG 5 cut(s) 802, 1436, 4938, 5102, 5879
BstEII GGTNACC 1 cut(s) 5555
BstFNI CGCG 2 cut(s) 426, 5881
BstH2I RGCGCY 2 cut(s) 4637, 5036
BstHHI GCGC 5 cut(s) 250, 428, 2556, 4636, 5035
BstMCI CGRYCG 2 cut(s) 138, 5596
BstNSI RCATGY 6 cut(s) 1899, 2424, 2465, 3266, 4326, 4589
BstPAI GACNNNNGTC 1 cut(s) 3598
BstPI GGTNACC 1 cut(s) 5555
BstSFI CTRYAG 8 cut(s) 717, 1476, 2391, 2849, 3828, 5065, 5386, 5748
BstSLI GKGCMC 2 cut(s) 1444, 3411
BstUI CGCG 2 cut(s) 426, 5881
BstX2I RGATCY 6 cut(s) 3584, 5110, 5255, 5480, 5708, 5717
BstXI CCANNNNNNTGG 2 cut(s) 2078, 4119
BstYI RGATCY 6 cut(s) 3584, 5110, 5255, 5480, 5708, 5717
Bsu15I ATCGAT 2 cut(s) 847, 4281
BsuI GTATCC 1 cut(s) 4831
BsuTUI ATCGAT 2 cut(s) 847, 4281
BtgI CCRYGG 5 cut(s) 802, 1436, 4938, 5102, 5879
BtgZI GCGATG 2 cut(s) 174, 4631
BtsI GCAGTG 5 cut(s) 232, 2804, 3688, 4600, 5745
BveI ACCTGC 2 cut(s) 2048, 2398
CaiI CAGNNNCTG 3 cut(s) 2111, 3101, 5789
CfoI GCGC 5 cut(s) 250, 428, 2556, 4636, 5035
Cfr10I RCCGGY 2 cut(s) 3819, 5120
Cfr42I CCGCGG 1 cut(s) 5882
ClaI ATCGAT 2 cut(s) 847, 4281
CpoI CGGWCCG 1 cut(s) 83
CseI GACGC 1 cut(s) 4363
CspAI ACCGGT 2 cut(s) 3819, 5120
CspCI CAANNNNNGTGG 8 cut(s) 461, 496, 3364, 3399, 3417, 3452, 4059, 4094
CspI CGGWCCG 1 cut(s) 83
DraI TTTAAA 2 cut(s) 1179, 2007
DraIII CACNNNGTG 3 cut(s) 548, 3728, 4517
DriI GACNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 7 cut(s) 277, 958, 1096, 2377, 3745, 4180, 4783
Eam1104I CTCTTC 5 cut(s) 871, 1525, 3936, 4042, 4470
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 5 cut(s) 871, 1525, 3936, 4042, 4470
EciI GGCGGA 1 cut(s) 5708
Eco130I CCWWGG 2 cut(s) 5046, 5102
Eco147I AGGCCT 1 cut(s) 2739
Eco24I GRGCYC 2 cut(s) 80, 1444
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 3 cut(s) 580, 2233, 3304
Eco47I GGWCC 5 cut(s) 83, 1136, 1889, 2186, 3521
Eco47III AGCGCT 1 cut(s) 4635
Eco57I CTGAAG 7 cut(s) 1292, 1506, 3628, 3888, 4152, 4735, 4863
Eco88I CYCGRG 1 cut(s) 2973
Eco91I GGTNACC 1 cut(s) 5555
EcoO109I RGGNCCY 1 cut(s) 1441
EcoO65I GGTNACC 1 cut(s) 5555
EcoRI GAATTC 1 cut(s) 1258
EcoRV GATATC 3 cut(s) 580, 2233, 3304
EcoT14I CCWWGG 2 cut(s) 5046, 5102
EcoT22I ATGCAT 5 cut(s) 1771, 1879, 3582, 4063, 4328
EcoT38I GRGCYC 2 cut(s) 80, 1444
ErhI CCWWGG 2 cut(s) 5046, 5102
Esp3I CGTCTC 1 cut(s) 840
FaqI GGGAC 5 cut(s) 1302, 2135, 3061, 3320, 4094
FauI CCCGC 4 cut(s) 46, 1985, 5085, 5391
FauNDI CATATG 3 cut(s) 3676, 3930, 5681
FblI GTMKAC 3 cut(s) 2848, 3120, 4476
FriOI GRGCYC 2 cut(s) 80, 1444
GlaI GCGC 5 cut(s) 249, 427, 2555, 4635, 5034
GsaI CCCAGC 3 cut(s) 2333, 3204, 5759
GsuI CTGGAG 8 cut(s) 134, 339, 1228, 2613, 3510, 5039, 5583, 5715
HaeII RGCGCY 2 cut(s) 4637, 5036
HapII CCGG 7 cut(s) 81, 134, 280, 3820, 5121, 5156, 5253
HgaI GACGC 1 cut(s) 4363
HhaI GCGC 5 cut(s) 250, 428, 2556, 4636, 5035
Hin1I GRCGYC 1 cut(s) 99
Hin6I GCGC 5 cut(s) 248, 426, 2554, 4634, 5033
HinP1I GCGC 5 cut(s) 248, 426, 2554, 4634, 5033
HincII GTYRAC 4 cut(s) 103, 3425, 5074, 5551
HindII GTYRAC 4 cut(s) 103, 3425, 5074, 5551
HindIII AAGCTT 3 cut(s) 353, 4881, 5223
HpaII CCGG 7 cut(s) 81, 134, 280, 3820, 5121, 5156, 5253
Hpy99I CGWCG 1 cut(s) 244
HpyCH4IV ACGT 9 cut(s) 99, 624, 1078, 2356, 2473, 3993, 5770, 5835, 5906
HpySE526I ACGT 9 cut(s) 99, 624, 1078, 2356, 2473, 3993, 5770, 5835, 5906
Hsp92I GRCGYC 1 cut(s) 99
HspAI GCGC 5 cut(s) 248, 426, 2554, 4634, 5033
Kpn2I TCCGGA 1 cut(s) 5252
KpnI GGTACC 1 cut(s) 5180
KspI CCGCGG 1 cut(s) 5882
LguI GCTCTTC 1 cut(s) 3936
MaeII ACGT 9 cut(s) 99, 624, 1078, 2356, 2473, 3993, 5770, 5835, 5906
MbiI CCGCTC 1 cut(s) 5398
MfeI CAATTG 3 cut(s) 261, 2439, 2644
MflI RGATCY 6 cut(s) 3584, 5110, 5255, 5480, 5708, 5717
MhlI GDGCHC 8 cut(s) 80, 1444, 2700, 2791, 3017, 3411, 3867, 4702
MlsI TGGCCA 4 cut(s) 960, 2379, 3747, 4182
MluNI TGGCCA 4 cut(s) 960, 2379, 3747, 4182
MmeI TCCRAC 7 cut(s) 1445, 3876, 4653, 4769, 4973, 5257, 5642
Mox20I TGGCCA 4 cut(s) 960, 2379, 3747, 4182
Mph1103I ATGCAT 5 cut(s) 1771, 1879, 3582, 4063, 4328
MroI TCCGGA 1 cut(s) 5252
MroXI GAANNNNTTC 5 cut(s) 1062, 1124, 2988, 3912, 5906
MscI TGGCCA 4 cut(s) 960, 2379, 3747, 4182
MslI CAYNNNNRTG 8 cut(s) 968, 1115, 1595, 2076, 2166, 2466, 3261, 3933
Msp20I TGGCCA 4 cut(s) 960, 2379, 3747, 4182
MspA1I CMGCKG 4 cut(s) 487, 2410, 5786, 5881
MspI CCGG 7 cut(s) 81, 134, 280, 3820, 5121, 5156, 5253
MunI CAATTG 3 cut(s) 261, 2439, 2644
Mva1269I GAATGC 5 cut(s) 1769, 1867, 2046, 3724, 4326
MvnI CGCG 2 cut(s) 426, 5881
NcoI CCATGG 1 cut(s) 5102
NdeI CATATG 3 cut(s) 3676, 3930, 5681
NheI GCTAGC 1 cut(s) 2449
NmeAIII GCCGAG 1 cut(s) 1901
NsiI ATGCAT 5 cut(s) 1771, 1879, 3582, 4063, 4328
NspI RCATGY 6 cut(s) 1899, 2424, 2465, 3266, 4326, 4589
NspV TTCGAA 1 cut(s) 4729
PaeI GCATGC 1 cut(s) 4326
PaeR7I CTCGAG 1 cut(s) 2973
PaqCI CACCTGC 1 cut(s) 2048
PceI AGGCCT 1 cut(s) 2739
PciI ACATGT 2 cut(s) 2420, 2461
PciSI GCTCTTC 1 cut(s) 3936
PctI GAATGC 5 cut(s) 1769, 1867, 2046, 3724, 4326
PdmI GAANNNNTTC 5 cut(s) 1062, 1124, 2988, 3912, 5906
PflMI CCANNNNNTGG 6 cut(s) 3320, 3530, 4041, 4430, 4832, 5525
PinAI ACCGGT 2 cut(s) 3819, 5120
Ple19I CGATCG 1 cut(s) 5596
PscI ACATGT 2 cut(s) 2420, 2461
PshAI GACNNNNGTC 1 cut(s) 3598
PshBI ATTAAT 1 cut(s) 3297
Psp1406I AACGTT 1 cut(s) 3993
PspEI GGTNACC 1 cut(s) 5555
PspFI CCCAGC 3 cut(s) 2329, 3200, 5755
PspOMI GGGCCC 1 cut(s) 1440
PsrI GAACNNNNNNTAC 2 cut(s) 2388, 2420
PstI CTGCAG 2 cut(s) 2395, 5752
PstNI CAGNNNCTG 3 cut(s) 2111, 3101, 5789
PsuI RGATCY 6 cut(s) 3584, 5110, 5255, 5480, 5708, 5717
PvuI CGATCG 1 cut(s) 5596
PvuII CAGCTG 2 cut(s) 487, 5786
RseI CAYNNNNRTG 8 cut(s) 968, 1115, 1595, 2076, 2166, 2466, 3261, 3933
Rsr2I CGGWCCG 1 cut(s) 83
RsrII CGGWCCG 1 cut(s) 83
SacII CCGCGG 1 cut(s) 5882
SapI GCTCTTC 1 cut(s) 3936
ScaI AGTACT 2 cut(s) 2979, 5014
SduI GDGCHC 8 cut(s) 80, 1444, 2700, 2791, 3017, 3411, 3867, 4702
SfcI CTRYAG 8 cut(s) 717, 1476, 2391, 2849, 3828, 5065, 5386, 5748
Sfr274I CTCGAG 1 cut(s) 2973
Sfr303I CCGCGG 1 cut(s) 5882
SfuI TTCGAA 1 cut(s) 4729
SgrBI CCGCGG 1 cut(s) 5882
SinI GGWCC 5 cut(s) 83, 1136, 1889, 2186, 3521
SlaI CTCGAG 1 cut(s) 2973
SmiMI CAYNNNNRTG 8 cut(s) 968, 1115, 1595, 2076, 2166, 2466, 3261, 3933
SmlI CTYRAG 9 cut(s) 674, 816, 1352, 2626, 2810, 2973, 3307, 4610, 4866
SmoI CTYRAG 9 cut(s) 674, 816, 1352, 2626, 2810, 2973, 3307, 4610, 4866
SpeI ACTAGT 1 cut(s) 227
SphI GCATGC 1 cut(s) 4326
SseBI AGGCCT 1 cut(s) 2739
SspI AATATT 1 cut(s) 3253
StuI AGGCCT 1 cut(s) 2739
StyI CCWWGG 2 cut(s) 5046, 5102
TaiI ACGT 9 cut(s) 102, 627, 1081, 2359, 2476, 3996, 5773, 5838, 5909
TatI WGTACW 5 cut(s) 664, 2307, 2977, 3394, 5012
TauI GCSGC 3 cut(s) 2030, 5139, 5881
TspGWI ACGGA 8 cut(s) 258, 791, 859, 1425, 1664, 2500, 2513, 4927
Van91I CCANNNNNTGG 6 cut(s) 3320, 3530, 4041, 4430, 4832, 5525
VpaK11BI GGWCC 5 cut(s) 83, 1136, 1889, 2186, 3521
VspI ATTAAT 1 cut(s) 3297
XapI RAATTY 8 cut(s) 1258, 2128, 3341, 3414, 3474, 4016, 4458, 4776
XceI RCATGY 6 cut(s) 1899, 2424, 2465, 3266, 4326, 4589
XcmI CCANNNNNNNNNTGG 4 cut(s) 158, 846, 5296, 5359
XhoI CTCGAG 1 cut(s) 2973
XmiI GTMKAC 3 cut(s) 2848, 3120, 4476
XmnI GAANNNNTTC 5 cut(s) 1062, 1124, 2988, 3912, 5906
ZraI GACGTC 1 cut(s) 100
ZrmI AGTACT 2 cut(s) 2979, 5014
Zsp2I ATGCAT 5 cut(s) 1771, 1879, 3582, 4063, 4328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.