FvH4_6g51430

Bacterial Ig-like domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014163
Physical Location & Seq
Forward (+)
38124116 .. 38135829
11714 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51430.t1

Sequence Viewer

Length: 5838 bp
ATGTCGAGCTTGGCGTTTTTACTCGGTTTGATTCTTTTGACCATGGCGGACCAAACGGCGTCGCATTTGAGCTCCGGTGGTCCGCACATCGCCGACGTCAATATCCTGCTGCCTCCGAAGATGACGCATCCGGTTGAGTATAGGCTTCAGGGAAGCGACGGCTGCTTCAAATGGTCGTGGGATCATCATGATATTCTATCTGTTCTGCCTGAATATAATGCAACTGGTCACTGCTCAACGAGTGCAAGGTTGACGTCTGTTGCTCCTTATAGTGGGAGGAAGGAGACTGCTGTTTATGCTACTGATGTTAATACTGGAGTTGTGATTCGATGCAAAGTTTTTATTGACAAACTTTCCAGAATCCAGATATTTCATAATTCTGTTAAACTTGACTTAGATGGGCTTGCCACTCTTCAAGTCCGCGCCTTTGATGATGAAGAAAATGTGTTTTCGTCACTGGTGGGATTGCAATTCATGTGGCAGCTATTATCTGAAACCAATGTACATCACCTCGTTCATGTTCCTCTTAAGGACTCTCCCCTGAGTGACTGTGGTGGATTGTGTGGAGACTTAGATGTCCAAATAAAACTTGAAGATAATGGTGTATTCTCTGACATGTATGTTGTAAAAGGAATCGAAATTGGGCATGAGATTGTTTCTGTGCATTTGCTTGAGCCACAATTCAAGCACATGGCTGATAAGATTGTCCTAACCGTGGCAGAAGCCATGTCACTTGAACCTCCCTCACCTGTACTTGTCCTTGTTGGCGCTGCTGTTCGTTATAATCTGAAAGTCATTCGTGAGAACAAGGCTCAAGTGGTAAATTTGCCGTCTCCACATCATCAATGGTCTTCGTCAAACTCTTCAGTTGCTAATGTTGACTCTACGTCAGGATTGACAAACGCATTGAGCTTAGGGGTTACAAATGTTATAGTTGAAGATACAAGGGTTACTGGCCACATACAAGTGTCCTCACTGAATGTTGTCCTGCCAGATTCTTTATCTTTATACATGACACCTCTTTCTGCTTCTGGTGATCCTGTACAAGGAACTAAAGCGATTCCGTCCATGACCCGTTGGTTTGGTGTTTCTGGTCATCAGTACCTCATTCAAATGAAGGTTTTCTCCCAGGGGCCAGATGCACAAGAAATTTACATTACAGAGAATGATGATCTAAAGCTGTCCAAAACCCATTCAGATTATTGGCAAATTTTTCCTGTATCAGATGATATTGCAGTCAAACATAGCTGGCAGAATTCTGTAGTCCTAAAAGCAACTTCATGGGGACAGGGAAAATTGACAGCTTCATTGACGTACTTTAGTGCTCTAGATGAAACGAAGGAGGTTCTCAAGGTTGTGCAAGAACTCACTATATGTGATCAAGTGAAGTTCAGTTTGAACAAAACTGTTGCTTCTCCAACCATTCTCCTTCCTTGGGTCCCTTCTATTTATCAGGAGGTCGAGCTGAAGGTTTCAGGAGGTTGTGCAAAAGCCTCGACAGACTACAAGTGGTACTCCTCAGACATGGGTATTGTGTCTGTATCTGCTTCTGGTGTTGTCCAGGCTAAGAAGCCTGGTAAAGCTACCATTAAAGTGCTATCCATTTTCGATTCATTCAATTATGATGAGGTGGTCATTGAAGTTTCTGTCCCCACATCTATGTCCATGCTTCCTAACTTCCCTGTGGAGACTGTTGTAGGATCACATCTTCAAGCTGCTGTAACAATGAAAGCATCAAATGGTGCCTACTTTTATAGATGTGATGCTTTCAGTTCCATCGTAAGGTGGAAGGTAGGGAGTGGGCCTTTCAACATCGTCAAAGGAGAAGCAGCTGATTTACACATGTTAGGGAGTGCTGAGTTTCATACATCAAGTTATGGTGCTCCATGTTCATGGGCAGAGTTATATGCTTCTGCTTCTGGTCGAGCAACTCTTCATGCTACATTACCTAATGAATATCACAACTCTGGAAGCTCTTTTCATGGACCTATTGTCTTAAAAGCATCTTCACTTATTGGGGCATATCCACCACTTAATGTTCGTCAGGCTGGTGATGGAAATCATTATGGTGGTTACTTTTTTGATTTGGCTCTAACAGAAACTGACAATCCTTTGGTAAAGTTGGATAAAGTGTACCTTGTGCCTGGAACGTGTCTGGATATCATGCTTCTTGGTGGTCCCGAACAGTGGAAGATTGGTGTCGAGTTTGTGGAAACTGTGGAAATCTTAAATAAAGAACATGGTCACACTGATGATGGTGCTTCTGTGCAACGATTATCTGAGACCTATAGGAGTCTGTACAGGGTTTCATGCGAGATGCTTGGAACCTATAATATTGTTTTCAAACGTGGTAATCTTGTTGGGGAAGACCATCCTATGCCTGCAGTGGCTGACGTGTTGATGTCTCTTATATGTAGCATTCCTACCTCGATTGTTATGATAGCTGATGAACCTGTAAATCATCTTGAAGTTATAAGGACAGCAATTCAGGCTGACCGTAGTTCAGGAAGAATTCGTGTCACTCCCATTACAGTGGCAAATAACCGCACCATTAGATTAGCTGCAGTTGGCATTAGTAGTAATGGAGAAGCTTTTGGAAATTCATCATCTCTTCATTTGCAGTGGGAACTTAACAGTTGTGATGGGCTGGCCTATTGGGATGATGCTGATAATTTACAGAGACCAAAATACAGTTGGGAAAAATTTTTGAGCCTGCAAAATGTGTCGGGAGTGTGTATTGTTCGTGCTACCGCTATCGGTTTCTACAATACCATGGGTCATCACCTTGAGAGTTCAGAGAATGCTCTTACAGATGCTATCCATTTGCAACTTGTGTCTACATTGAGGATTAGTCCAGAGTTTCATTTGGTAGTTTTCAATCCCAATGCCAAGGTAAACCTGGCAATTACTGGTGGGAGCTGTTTCTTGAAAGTTGGTGTAAATGATTCTCAGGTAGTAGAAGTCATTCAACCCCCAACCGATTTACAATGCTCACAACTGGTGCTGTCTCCTAAAGGTCTGGGGACTGCACTTGTGACAGTTAAGGATATTGGGCTTGCTCCTCCACTTGCAGCTTCTGCTGTGGTGCAAGTTGCCGAGATCGACTGGATAAAGATTGTATCACCAGAAGTTATATGCCTTATGGAAGGGAATTCACAGACAATTGATATAGTGGCCGGTATCAGTGATGGGAGAACTTTTGACTCTTATCAGTTTGCCTACATTAATATTCAAGTGCATGTTGAGGATCAAATAATTGAGGTTTTAGATATTAATTCAAACACAGGTGGTGGATACATAAATGTCCCAGAATTTAAGATTTTTGCTTCTCATCTTGGGATCACAACTTTCTTTGTCAGTGCTATGCAGCAATCTGGGCATGAAATTTTCAGTCAACCAATAATGGTGGAAGTCTATGCAGCACCAGAGATTCACCCCCATGATATATTCCTTGTACCTGGTGCATCTTATGTGCTTACTTTAAAGGGAGGCCCAACACTGGGTGTAAATGTTGAATATACGAGCATGGATGATGAAGTAGCAACAATAGATAGATCTTCAGGACGATTATCTGCGAGTTTACCTGGGAACACTACTATTAGTGCCACTGTCTTAAAAAATGGAGAAACTGTGATCTGTCGAGCATATACCACTGTTAAAGTGGGAGTTCCTTCTTCGGTGATATTGAATGCTCAAAGTGAACTGCTTGGTGTCGGCAAAGAAATGCCACTTTATCCTGTGTTTTCTGAGGGTGATCTGTTTTCTGTTTATGAGCAATGCCAAGATTACCATTGGAGCGGAGAAGATGAAAAGGTGTTGAGTTTTTATGGGCTAGAGCACTTGAATAGTGAAAAGTATGGGTCTCAACTAGACTATGCAGAAAAGTTTCGTTTTACAAGCCATATTAGTGAGGAAGACCTTGGTTTTATCAAAGTAGTGCTTGGAAGATCTGCAGGGAGAACAAATGTCGCAGTTTCATTCTCATGTGAATTTGTATCTTCCGGTTCTAAGTCATGGAGAAGAATTTACAATGCCTCGGTATCAATATCGGTGGTGCCTGATCCCCCACTTGCGCTTGGAGTTCCAATAACCTGGATTCTTCCTCCTCATTATACCACAAGTAGTCTTTTACCTTTATCTTCGGAATTGCATGGCCAATGGGATACTCAGAGTCATAAAGGAACTATCATCTATTCTTTATTGAGAAATGTTCCTTACAAAAATGAAGTTCTGCAAAAAGATGTTATTTCCATTGAGGGAGACAGAATAAAGACATCAGAAAGTAATAATCTCGCATGCATTCAGGCAAAAGATCGAATGACAGGTAGAATTGAGATTGCTGCTTGTGTCAAAGTTGCTGAGGTGGCTCAAATAAGGATTTCAGATGACTGGTTACCATTTCGCGGGGTCAATCTTGTTCTTGGTGCTGAGCTTTCTCTTCCAATAGTCTATTTGGACGCACTAGGAAACCGTTTTCATGAAGCATATGACATTGTCCTCTTTGATGCTGAAACTGACAATCCTGATGTTGTGTCTGTAAACACGACACTCGGTGGAAGTGGAATCATTCATCTCAAGGCAATGCGACATGGTAGAGCTCTTGTGCGAGTATCCATTGCTAGTATGCCCTTGAAGTCCGACTATATCCTGATATCAGTGGGAGCGCATATACATCCTCAGAACCCAGTTATTCATATTGGAAGCCATGTTAACTTCAGTATAGAAGGTTTAAATGATCAAATTTCTGGCCGGTGGCTCACTGCCAATGAAAGTGTCATATCTGTCTCCCCATTGTCTGGAGAAGCTGAAGTAATTGGGGAAGGTTCAACTCAAGTACACTTTGAAGCGTTAAGCATGAAGTTGCGGACAACAGTTACAGTGCTAACAGATGATATTGTTTCTGTTGATGCTCCAAGAGAGACACTGACAAATGTTCCCTTCCCCACTAAAGGATATAACTTCTCTGTGAAGATCAGCGACAAGTTTAAGGCATTTGGAAACACCAAGGGACTCCAGTATGTCTGTAGAGTGGACCCCCCTTTTGTTGGGTATTCAAATCCATGGATTGATCTTGATACTGGTAACTCATACTGCCTATTCTTCCCTTACACACCGGAGCATTTGGTTCGTTTCAAGTCCAAAGAGATGAAACCAGATATAACTGTTTCCATCAATGCTTCACTTAGAGGAGCTGATCATGTGTCAGGATCTGCATCTGCTCTTTTTGTTGGAGGGTTTTCTGTGTTGGAGATGGGCAAGGATTCAGTCCAGTTGAATCTGACTCCAGACTCTAACAAGACTATTATCACAATTCTGGGGAACACAGATGTTGAAATTTATTGGCATGACCGGGATTTATTACTCGTTACTCCCATCCATAAAGAAGGTTTTGGGATCGGTGGGCGTGCAAAGTATGAGGTCAGAATGCTAGGTACCAAGAGATTCAAAGATACAATTTTCATCACACTCCCATCCAATGGTCAAAGTGTGGAAATCTACGTCAATAGTGACCCTGGAGAGACACCAGCATCAGAAACTACCACTATCAGTTATACATTTTGGCCAACCGTTCTCGGAGGCCTTGCAATACTGATTTTGATAGTGGTAGTCTTCAAGTATTATTCTGACAAGCCTGATAGATCTCATATACCGGTTGCTCCTGCTACTCCAAGTATGGCAGCCCCAATCACACCTGAACGTGGCAGCCCTGCCGATGTGAGTGATCTGTCTCCTCGAACACCTCAGCCCTTCATGGATTATGTGAGGAGGACGATAGATGAAACCCCATACTACAGGCGAGAGCCACGAAGGAGGGTTAACCCACAAAATACTTTCTAG

Protein Analysis

1946

Amino Acids

212.61

Weight (kDa)

5.5

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_NUP210_1st PF22967 34 - 116 5.5e-17 NUP210 Ig-like domain 1
Ig_NUP210_2nd PF22969 136 - 232 1.4e-11 NUP210 Ig-like domain 2
Ig_NUP210_3rd PF22963 243 - 319 1.8e-06 NUP210 Ig-like domain 3
Ig_NUP210_5th PF26182 474 - 548 2e-06 NUP210 Ig-like domain 5
Big_2 PF02368 486 - 534 8.4e-07 Bacterial Ig-like domain (group 2)
Ig_NUP210_7th PF22962 709 - 806 9.8e-15 NUP210 Ig-like domain 7
Big_2 PF02368 1144 - 1210 5.4e-08 Bacterial Ig-like domain (group 2)
Ig_GP210_15th PF24425 1630 - 1743 1.2e-48 Nuclear pore complex protein GP210, Ig-like domain
Ig_GP210_16th PF24427 1755 - 1835 1.7e-36 Nuclear pore complex protein GP210, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10590 AT5G40480
fragaria_vesca FvH4_6g51430
malus_domestica MD17G1026700.v1.1
prunus_persica Prupe.3G292000_v2.0.a1 Prupe.3G292000_v2.0.a1
pyrus_communis pycom17g02180
rosa_chinensis RchiOBHm_Chr2g0173101
rosa_laevigata RLG00000022158
rosa_multiflora Rmu_sc0011236.1_g000011
rosa_roxburghii Rroxscaffold_2G00079240
rosa_rugosa Rorug02G0565200
rosa_samantha Rh2AG645300 Rh2BG656000 Rh2CG621100 Rh2DG669400
rosa_wichuraiana Rw2G053000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 783, 2474
AasI GACNNNNNNGTC 1 cut(s) 575
AatII GACGTC 2 cut(s) 99, 257
Acc65I GGTACC 1 cut(s) 5432
AccB1I GGYRCC 3 cut(s) 1742, 4054, 5432
AccB7I CCANNNNNTGG 2 cut(s) 4796, 5477
AccBSI CCGCTC 1 cut(s) 3798
AccI GTMKAC 1 cut(s) 2837
AccII CGCG 2 cut(s) 423, 4404
AciI CCGC 8 cut(s) 47, 83, 421, 2545, 2751, 3798, 4404, 4864
AclWI GGATC 8 cut(s) 189, 1031, 1708, 3255, 3347, 4055, 5215, 5402
AcoI YGGCCR 5 cut(s) 955, 3174, 4153, 4747, 5561
AcuI CTGAAG 6 cut(s) 131, 849, 1487, 3543, 4699, 4827
AcyI GRCGYC 3 cut(s) 59, 96, 254
AdeI CACNNNGTG 2 cut(s) 3503, 4553
AflII CTTAAG 1 cut(s) 527
AflIII ACRYGT 4 cut(s) 615, 1842, 2150, 2394
AgeI ACCGGT 1 cut(s) 5650
AhdI GACNNNNNGTC 2 cut(s) 886, 1991
AjiI CACGTC 1 cut(s) 2395
AjnI CCWGG 9 cut(s) 1128, 1560, 1573, 2143, 2898, 3457, 3583, 4091, 5512
Alw21I GWGCWC 5 cut(s) 74, 1327, 1885, 3840, 4600
AlwI GGATC 8 cut(s) 189, 1031, 1708, 3255, 3347, 4055, 5215, 5402
AlwNI CAGNNNCTG 4 cut(s) 2102, 2390, 3077, 5210
ArsI GACNNNNNNTTYG 2 cut(s) 5024, 5056
AseI ATTAAT 2 cut(s) 3225, 3273
AsiGI ACCGGT 1 cut(s) 5650
Asp700I GAANNNNTTC 3 cut(s) 1121, 2964, 3885
Asp718I GGTACC 1 cut(s) 5432
AspLEI GCGC 4 cut(s) 425, 770, 4075, 4666
AspS9I GGNCC 9 cut(s) 49, 80, 1133, 1438, 1802, 1985, 2177, 3491, 5032
AsuC2I CCSGG 1 cut(s) 5351
AsuHPI GGTGA 9 cut(s) 500, 738, 1046, 2063, 2774, 3114, 3425, 3691, 3764
AvaII GGWCC 6 cut(s) 49, 80, 1438, 1985, 2177, 5032
BaeI ACNNNNGTAYC 2 cut(s) 933, 966
BalI TGGCCA 3 cut(s) 957, 4155, 5563
BanI GGYRCC 3 cut(s) 1742, 4054, 5432
BanII GRGCYC 2 cut(s) 74, 4600
BbsI GAAGAC 4 cut(s) 843, 2373, 3921, 5602
Bbv12I GWGCWC 5 cut(s) 74, 1327, 1885, 3840, 4600
BbvCI CCTCAGC 2 cut(s) 4359, 5742
BceAI ACGGC 3 cut(s) 72, 175, 814
BcgI CGANNNNNNTGC 4 cut(s) 1476, 1510, 5108, 5142
BciT130I CCWGG 9 cut(s) 1130, 1562, 1575, 2145, 2900, 3459, 3585, 4093, 5514
BciVI GTATCC 3 cut(s) 3287, 4156, 4621
BclI TGATCA 3 cut(s) 1378, 4735, 5194
BcnI CCSGG 1 cut(s) 5351
BfaI CTAG 7 cut(s) 1328, 3833, 3869, 4463, 4620, 5429, 5836
BfmI CTRYAG 7 cut(s) 1260, 2287, 2382, 2562, 3951, 5023, 5791
BfoI RGCGCY 1 cut(s) 771
BfrI CTTAAG 1 cut(s) 527
BfuI GTATCC 3 cut(s) 3287, 4156, 4621
BglII AGATCT 3 cut(s) 3554, 3947, 5639
BlpI GCTNAGC 1 cut(s) 4428
Bme18I GGWCC 6 cut(s) 49, 80, 1438, 1985, 2177, 5032
BmeRI GACNNNNNGTC 2 cut(s) 886, 1991
BmgBI CACGTC 1 cut(s) 2395
BmgT120I GGNCC 9 cut(s) 49, 80, 1133, 1438, 1802, 1985, 2177, 3491, 5032
BmiI GGNNCC 9 cut(s) 1134, 1439, 1440, 1744, 2179, 2326, 4056, 5034, 5434
BmrI ACTGGG 2 cut(s) 3509, 4679
BmuI ACTGGG 2 cut(s) 3509, 4679
BpiI GAAGAC 4 cut(s) 843, 2373, 3921, 5602
BpmI CTGGAG 5 cut(s) 336, 4818, 4997, 5268, 5535
Bpu10I CCTNAGC 3 cut(s) 913, 4359, 5742
Bpu1102I GCTNAGC 1 cut(s) 4428
BpuEI CTTGAG 6 cut(s) 692, 798, 1334, 2807, 4559, 4815
BpuMI CCSGG 1 cut(s) 5351
BsaBI GATNNNNATC 2 cut(s) 2058, 5212
BsaHI GRCGYC 3 cut(s) 59, 96, 254
BsaI GGTCTC 3 cut(s) 2276, 2674, 3867
BsaWI WCCGGW 5 cut(s) 74, 130, 4001, 5113, 5650
BsaXI ACNNNNNCTCC 2 cut(s) 3615, 3645
Bse118I RCCGGY 3 cut(s) 3176, 4749, 5650
Bse3DI GCAATG 3 cut(s) 3782, 4587, 4614
Bse8I GATNNNNATC 2 cut(s) 2058, 5212
BseBI CCWGG 9 cut(s) 1130, 1562, 1575, 2145, 2900, 3459, 3585, 4093, 5514
BseGI GGATG 7 cut(s) 127, 2371, 2665, 3535, 4672, 5373, 5471
BseJI GATNNNNATC 2 cut(s) 2058, 5212
BseMI GCAATG 3 cut(s) 3782, 4587, 4614
BseRI GAGGAG 6 cut(s) 1507, 3051, 4095, 5202, 5721, 5779
BsgI GTGCAG 1 cut(s) 3012
Bsh1236I CGCG 2 cut(s) 423, 4404
BshNI GGYRCC 3 cut(s) 1742, 4054, 5432
BshTI ACCGGT 1 cut(s) 5650
BsiHKAI GWGCWC 5 cut(s) 74, 1327, 1885, 3840, 4600
BsiSI CCGG 8 cut(s) 75, 131, 3177, 4002, 4750, 5114, 5350, 5651
BslFI GGGAC 7 cut(s) 1299, 1424, 1634, 2163, 3037, 3290, 5022
BsmBI CGTCTC 1 cut(s) 837
BsmFI GGGAC 7 cut(s) 1299, 1424, 1634, 2163, 3037, 3290, 5022
BsmI GAATGC 5 cut(s) 2418, 2806, 3694, 4299, 5430
Bso31I GGTCTC 3 cut(s) 2276, 2674, 3867
Bsp1286I GDGCHC 5 cut(s) 74, 1327, 1885, 3840, 4600
Bsp1407I TGTACA 3 cut(s) 502, 1042, 2298
Bsp1720I GCTNAGC 1 cut(s) 4428
Bsp19I CCATGG 3 cut(s) 42, 2772, 5060
BspACI CCGC 8 cut(s) 47, 83, 421, 2545, 2751, 3798, 4404, 4864
BspFNI CGCG 2 cut(s) 423, 4404
BspHI TCATGA 2 cut(s) 187, 4477
BspLI GGNNCC 9 cut(s) 1134, 1439, 1440, 1744, 2179, 2326, 4056, 5034, 5434
BspMAI CTGCAG 3 cut(s) 2386, 2566, 3955
BspPI GGATC 8 cut(s) 189, 1031, 1708, 3255, 3347, 4055, 5215, 5402
BspT107I GGYRCC 3 cut(s) 1742, 4054, 5432
BspTI CTTAAG 1 cut(s) 527
BspTNI GGTCTC 3 cut(s) 2276, 2674, 3867
BsrBI CCGCTC 1 cut(s) 3798
BsrDI GCAATG 3 cut(s) 3782, 4587, 4614
BsrFI RCCGGY 3 cut(s) 3176, 4749, 5650
BsrGI TGTACA 3 cut(s) 502, 1042, 2298
BssAI RCCGGY 3 cut(s) 3176, 4749, 5650
BssNI GRCGYC 3 cut(s) 59, 96, 254
BssT1I CCWWGG 7 cut(s) 42, 1433, 2772, 2889, 3919, 5004, 5060
Bst2UI CCWGG 9 cut(s) 1130, 1562, 1575, 2145, 2900, 3459, 3585, 4093, 5514
Bst6I CTCTTC 5 cut(s) 417, 868, 1938, 2616, 4443
BstACI GRCGYC 3 cut(s) 59, 96, 254
BstAFI CTTAAG 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 3077
BstAUI TGTACA 3 cut(s) 502, 1042, 2298
BstC8I GCNNGC 8 cut(s) 405, 1250, 2382, 2649, 2714, 3057, 4297, 5406
BstDSI CCRYGG 4 cut(s) 42, 714, 2772, 5060
BstEII GGTNACC 1 cut(s) 4392
BstENI CCTNNNNNAGG 1 cut(s) 1044
BstF5I GGATG 7 cut(s) 127, 2371, 2665, 3535, 4672, 5373, 5471
BstFNI CGCG 2 cut(s) 423, 4404
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 4 cut(s) 425, 770, 4075, 4666
BstMWI GCNNNNNNNGC 6 cut(s) 162, 296, 2489, 3077, 3376, 4364
BstNI CCWGG 9 cut(s) 1130, 1562, 1575, 2145, 2900, 3459, 3585, 4093, 5514
BstNSI RCATGY 4 cut(s) 619, 1846, 3242, 4299
BstPI GGTNACC 1 cut(s) 4392
BstSFI CTRYAG 7 cut(s) 1260, 2287, 2382, 2562, 3951, 5023, 5791
BstUI CGCG 2 cut(s) 423, 4404
BstV2I GAAGAC 4 cut(s) 843, 2373, 3921, 5602
BstX2I RGATCY 4 cut(s) 3554, 3947, 5207, 5639
BstXI CCANNNNNNTGG 3 cut(s) 1893, 2533, 4092
BstYI RGATCY 4 cut(s) 3554, 3947, 5207, 5639
BsuI GTATCC 3 cut(s) 3287, 4156, 4621
BtgI CCRYGG 4 cut(s) 42, 714, 2772, 5060
BtgZI GCGATG 1 cut(s) 73
BtrI CACGTC 1 cut(s) 2395
BtsCI GGATG 7 cut(s) 127, 2371, 2665, 3535, 4672, 5373, 5471
BtsI GCAGTG 4 cut(s) 229, 2391, 2627, 4758
Cac8I GCNNGC 8 cut(s) 405, 1250, 2382, 2649, 2714, 3057, 4297, 5406
CaiI CAGNNNCTG 4 cut(s) 2102, 2390, 3077, 5210
CciI TCATGA 2 cut(s) 187, 4477
CfoI GCGC 4 cut(s) 425, 770, 4075, 4666
Cfr10I RCCGGY 3 cut(s) 3176, 4749, 5650
Cfr13I GGNCC 9 cut(s) 49, 80, 1133, 1438, 1802, 1985, 2177, 3491, 5032
CseI GACGC 3 cut(s) 48, 133, 4466
CsiI ACCWGGT 1 cut(s) 3457
CspAI ACCGGT 1 cut(s) 5650
CspCI CAANNNNNGTGG 6 cut(s) 458, 493, 3387, 3422, 4032, 4067
DraI TTTAAA 2 cut(s) 3483, 4731
DraIII CACNNNGTG 2 cut(s) 3503, 4553
DrdI GACNNNNNNGTC 1 cut(s) 575
DriI GACNNNNNGTC 2 cut(s) 886, 1991
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 5 cut(s) 955, 3174, 4153, 4747, 5561
Eam1104I CTCTTC 5 cut(s) 417, 868, 1938, 2616, 4443
Eam1105I GACNNNNNGTC 2 cut(s) 886, 1991
EarI CTCTTC 5 cut(s) 417, 868, 1938, 2616, 4443
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 2 cut(s) 72, 4598
Eco130I CCWWGG 7 cut(s) 42, 1433, 2772, 2889, 3919, 5004, 5060
Eco147I AGGCCT 1 cut(s) 5580
Eco24I GRGCYC 2 cut(s) 74, 4600
Eco31I GGTCTC 3 cut(s) 2276, 2674, 3867
Eco32I GATATC 2 cut(s) 2161, 4653
Eco47I GGWCC 6 cut(s) 49, 80, 1438, 1985, 2177, 5032
Eco53kI GAGCTC 2 cut(s) 72, 4598
Eco57I CTGAAG 6 cut(s) 131, 849, 1487, 3543, 4699, 4827
Eco91I GGTNACC 1 cut(s) 4392
EcoICRI GAGCTC 2 cut(s) 72, 4598
EcoNI CCTNNNNNAGG 1 cut(s) 1044
EcoO109I RGGNCCY 1 cut(s) 1438
EcoO65I GGTNACC 1 cut(s) 4392
EcoRI GAATTC 3 cut(s) 1255, 2511, 3151
EcoRII CCWGG 9 cut(s) 1128, 1560, 1573, 2143, 2898, 3457, 3583, 4091, 5512
EcoRV GATATC 2 cut(s) 2161, 4653
EcoT14I CCWWGG 7 cut(s) 42, 1433, 2772, 2889, 3919, 5004, 5060
EcoT22I ATGCAT 1 cut(s) 4301
EcoT38I GRGCYC 2 cut(s) 74, 4600
ErhI CCWWGG 7 cut(s) 42, 1433, 2772, 2889, 3919, 5004, 5060
Esp3I CGTCTC 1 cut(s) 837
FalI AAGNNNNNCTT 4 cut(s) 2121, 2153, 3924, 3956
FaqI GGGAC 7 cut(s) 1299, 1424, 1634, 2163, 3037, 3290, 5022
FauI CCCGC 1 cut(s) 4397
FauNDI CATATG 1 cut(s) 4486
FbaI TGATCA 3 cut(s) 1378, 4735, 5194
FblI GTMKAC 1 cut(s) 2837
FokI GGATG 7 cut(s) 114, 2358, 2672, 3542, 4659, 5360, 5458
FriOI GRGCYC 2 cut(s) 74, 4600
FspBI CTAG 7 cut(s) 1328, 3833, 3869, 4463, 4620, 5429, 5836
GlaI GCGC 4 cut(s) 424, 769, 4074, 4665
GsuI CTGGAG 5 cut(s) 336, 4818, 4997, 5268, 5535
HaeII RGCGCY 1 cut(s) 771
HapII CCGG 8 cut(s) 75, 131, 3177, 4002, 4750, 5114, 5350, 5651
HgaI GACGC 3 cut(s) 48, 133, 4466
HhaI GCGC 4 cut(s) 425, 770, 4075, 4666
Hin1I GRCGYC 3 cut(s) 59, 96, 254
Hin6I GCGC 4 cut(s) 423, 768, 4073, 4664
HinP1I GCGC 4 cut(s) 423, 768, 4073, 4664
HincII GTYRAC 5 cut(s) 252, 880, 3395, 4711, 5818
HindII GTYRAC 5 cut(s) 252, 880, 3395, 4711, 5818
HindIII AAGCTT 1 cut(s) 2589
HpaI GTTAAC 2 cut(s) 4711, 5818
HpaII CCGG 8 cut(s) 75, 131, 3177, 4002, 4750, 5114, 5350, 5651
HphI GGTGA 9 cut(s) 500, 738, 1046, 2063, 2774, 3114, 3425, 3691, 3764
Hpy99I CGWCG 3 cut(s) 64, 98, 161
HpyCH4IV ACGT 9 cut(s) 96, 254, 887, 1313, 2150, 2347, 2394, 5499, 5698
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 296, 2489, 3077, 3376, 4364
HpySE526I ACGT 9 cut(s) 96, 254, 887, 1313, 2150, 2347, 2394, 5499, 5698
Hsp92I GRCGYC 3 cut(s) 59, 96, 254
HspAI GCGC 4 cut(s) 423, 768, 4073, 4664
KflI GGGWCCC 1 cut(s) 1438
KpnI GGTACC 1 cut(s) 5436
Ksp22I TGATCA 3 cut(s) 1378, 4735, 5194
KspAI GTTAAC 2 cut(s) 4711, 5818
MabI ACCWGGT 1 cut(s) 3457
MaeI CTAG 7 cut(s) 1328, 3833, 3869, 4463, 4620, 5429, 5836
MaeII ACGT 9 cut(s) 96, 254, 887, 1313, 2150, 2347, 2394, 5499, 5698
MbiI CCGCTC 1 cut(s) 3798
MfeI CAATTG 1 cut(s) 3162
MflI RGATCY 4 cut(s) 3554, 3947, 5207, 5639
MhlI GDGCHC 5 cut(s) 74, 1327, 1885, 3840, 4600
MlsI TGGCCA 3 cut(s) 957, 4155, 5563
MluNI TGGCCA 3 cut(s) 957, 4155, 5563
MlyI GAGTC 8 cut(s) 527, 875, 2302, 3197, 4180, 5004, 5275, 5282
MmeI TCCRAC 5 cut(s) 1442, 2103, 4662, 5209, 5226
Mox20I TGGCCA 3 cut(s) 957, 4155, 5563
Mph1103I ATGCAT 1 cut(s) 4301
MroXI GAANNNNTTC 3 cut(s) 1121, 2964, 3885
MscI TGGCCA 3 cut(s) 957, 4155, 5563
Msp20I TGGCCA 3 cut(s) 957, 4155, 5563
MspA1I CMGCKG 1 cut(s) 1832
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 8 cut(s) 75, 131, 3177, 4002, 4750, 5114, 5350, 5651
MunI CAATTG 1 cut(s) 3162
Mva1269I GAATGC 5 cut(s) 2418, 2806, 3694, 4299, 5430
MvaI CCWGG 9 cut(s) 1130, 1562, 1575, 2145, 2900, 3459, 3585, 4093, 5514
MvnI CGCG 2 cut(s) 423, 4404
MwoI GCNNNNNNNGC 6 cut(s) 162, 296, 2489, 3077, 3376, 4364
NciI CCSGG 1 cut(s) 5351
NcoI CCATGG 3 cut(s) 42, 2772, 5060
NdeI CATATG 1 cut(s) 4486
NlaIV GGNNCC 9 cut(s) 1134, 1439, 1440, 1744, 2179, 2326, 4056, 5034, 5434
NmeAIII GCCGAG 1 cut(s) 3121
NmuCI GTSAC 8 cut(s) 227, 453, 545, 729, 2243, 2518, 3034, 5507
NsiI ATGCAT 1 cut(s) 4301
NspI RCATGY 4 cut(s) 619, 1846, 3242, 4299
PaeI GCATGC 1 cut(s) 4299
PagI TCATGA 2 cut(s) 187, 4477
PasI CCCWGGG 1 cut(s) 1129
PceI AGGCCT 1 cut(s) 5580
PciI ACATGT 2 cut(s) 615, 1842
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 5 cut(s) 2418, 2806, 3694, 4299, 5430
PdmI GAANNNNTTC 3 cut(s) 1121, 2964, 3885
PflFI GACNNNGTC 1 cut(s) 4493
PflMI CCANNNNNTGG 2 cut(s) 4796, 5477
PinAI ACCGGT 1 cut(s) 5650
PleI GAGTC 8 cut(s) 527, 875, 2301, 3197, 4179, 5004, 5275, 5282
PpsI GAGTC 8 cut(s) 527, 875, 2301, 3197, 4179, 5004, 5275, 5282
PpuMI RGGWCCY 1 cut(s) 1438
PscI ACATGT 2 cut(s) 615, 1842
PshBI ATTAAT 2 cut(s) 3225, 3273
PsiI TTATAA 2 cut(s) 783, 2474
Psp124BI GAGCTC 2 cut(s) 74, 4600
Psp5II RGGWCCY 1 cut(s) 1438
Psp6I CCWGG 9 cut(s) 1128, 1560, 1573, 2143, 2898, 3457, 3583, 4091, 5512
PspEI GGTNACC 1 cut(s) 4392
PspGI CCWGG 9 cut(s) 1128, 1560, 1573, 2143, 2898, 3457, 3583, 4091, 5512
PspN4I GGNNCC 9 cut(s) 1134, 1439, 1440, 1744, 2179, 2326, 4056, 5034, 5434
PspPI GGNCC 9 cut(s) 49, 80, 1133, 1438, 1802, 1985, 2177, 3491, 5032
PspPPI RGGWCCY 1 cut(s) 1438
PstI CTGCAG 3 cut(s) 2386, 2566, 3955
PstNI CAGNNNCTG 4 cut(s) 2102, 2390, 3077, 5210
PsuI RGATCY 4 cut(s) 3554, 3947, 5207, 5639
PsyI GACNNNGTC 1 cut(s) 4493
PvuII CAGCTG 1 cut(s) 1832
SacI GAGCTC 2 cut(s) 74, 4600
Sau96I GGNCC 9 cut(s) 49, 80, 1133, 1438, 1802, 1985, 2177, 3491, 5032
SchI GAGTC 8 cut(s) 527, 875, 2302, 3197, 4180, 5004, 5275, 5282
SduI GDGCHC 5 cut(s) 74, 1327, 1885, 3840, 4600
SexAI ACCWGGT 1 cut(s) 3457
SfcI CTRYAG 7 cut(s) 1260, 2287, 2382, 2562, 3951, 5023, 5791
SinI GGWCC 6 cut(s) 49, 80, 1438, 1985, 2177, 5032
SmlI CTYRAG 7 cut(s) 527, 671, 813, 1349, 2786, 4574, 4830
SmoI CTYRAG 7 cut(s) 527, 671, 813, 1349, 2786, 4574, 4830
SphI GCATGC 1 cut(s) 4299
SseBI AGGCCT 1 cut(s) 5580
SsiI CCGC 8 cut(s) 47, 83, 421, 2545, 2751, 3798, 4404, 4864
SspI AATATT 2 cut(s) 2335, 3229
SspMI CTAG 7 cut(s) 1328, 3833, 3869, 4463, 4620, 5429, 5836
SstI GAGCTC 2 cut(s) 74, 4600
StuI AGGCCT 1 cut(s) 5580
StyI CCWWGG 7 cut(s) 42, 1433, 2772, 2889, 3919, 5004, 5060
TaiI ACGT 9 cut(s) 99, 257, 890, 1316, 2153, 2350, 2397, 5502, 5701
TatI WGTACW 5 cut(s) 502, 751, 1042, 2298, 4834
TseFI GTSAC 8 cut(s) 227, 453, 545, 729, 2243, 2518, 3034, 5507
Tsp45I GTSAC 8 cut(s) 227, 453, 545, 729, 2243, 2518, 3034, 5507
TspGWI ACGGA 1 cut(s) 1053
Tth111I GACNNNGTC 1 cut(s) 4493
Van91I CCANNNNNTGG 2 cut(s) 4796, 5477
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 6 cut(s) 49, 80, 1438, 1985, 2177, 5032
VspI ATTAAT 2 cut(s) 3225, 3273
XagI CCTNNNNNAGG 1 cut(s) 1044
XbaI TCTAGA 1 cut(s) 1327
XceI RCATGY 4 cut(s) 619, 1846, 3242, 4299
XcmI CCANNNNNNNNNTGG 4 cut(s) 843, 2691, 2896, 3658
XmiI GTMKAC 1 cut(s) 2837
XmnI GAANNNNTTC 3 cut(s) 1121, 2964, 3885
XspI CTAG 7 cut(s) 1328, 3833, 3869, 4463, 4620, 5429, 5836
ZraI GACGTC 2 cut(s) 97, 255
Zsp2I ATGCAT 1 cut(s) 4301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.