Rh2BG656000

Bacterial Ig-like domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014163
Physical Location & Seq
Forward (+)
87334072 .. 87346565
12494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG656000.1

Sequence Viewer

Length: 5844 bp
ATGTCGCCAAGCTTCGCTTTTTTACTCGGCCTGGTTCTTTTGACTATGGCGGACCAAACGGCGTCGCATTTGAGCTCCGGCGGTCCGCACATTGCCGACGTCAATATCCTGCTGCCTCCGAAGATGACGCATCCGGTTGAGTATAGGCTTCAGGGAAGCGATGGCTGCTTCAAATGGTCATGGGATCATCACGATATTCTCTCTGTTCTGCCTGAATATAATTCAACTAGTCACTGCTCAACGAGTGCGCGGTTGAGATCAGTTGCTTCTTATAGTGGTAGGAAGGAGACTGCTGTTTATGCTACCGACGTAAATACTGGAGTTGTGATTCGGTGCAAAGTTTTTATTGACAAACTTTCCAGAATCCAGATATTTCATAATTCTGTTAAGCTTGACTTAGATGGGCTTGCCACTCTTCAAGTCCGTGCCTTTGATGATGAAGAAAATGTGTTTTCGTCACTGGTGGGATTGCAATTTATGTGGCAGCTAGAAACCAATGAACCACTGCATCACCTTGTTCATGTTCCTCTTAAGGACTCGCCCCTGAGTGACTGTGGTGGATTGTGTGGAGACCTAGATGTCCAAATAAAACTTGAAGATAGTGGTGTGTTCTCAGACATGTATGTTGTAAAGGGAATCGAAATTGGGCATGAGATTGTTTCTGTGCATTTGCTTGAGCCACAATTCAAGTACATGGCTGATACGATTGTCCTAACTGTAGCAGAAGCCATGTCACTTGAACCTCCCTCACCTGTACTTGTCCTTGTTGGCGCTGCTGTTTCTTATAATCTAAAAGTTATTCGTGGGAACAAGGCTCAAGTGGTAACTTTGCCGTCCCCACATCATCAATGGTCTTCGTCAAACTCTTCAGTTGCTCATGTTGACTCTCTGACAGGCTTGACAAACGCATTGAGCTTAGGGGTAACAAATGTCATAGTTGAAGATACAAGGGTTACTGGCCACATACAAGTGTCCTCACTCAATGTTGTCCTGCCAGATTCTTTGTCTTTATACTTGACACCTCTGTCTGCTTCTGGTGATCCTGTACAAGGAACTAAAGCAATTCCATCCATGACCCGTTGGTATGGTGTTTCTGGTCATCAGTACCTCATTCAAATGAAGGTTTTCTCCCAGGGGCCAGATGCGCAAGAAATTTACATCACAGAGAATGATGATCTAAAGCTGTCCAAAACCCAGTCAGATTATTGGCAAATTTTTCCTGTATCTGATGATATTGCAGTCAAACATAGCTGGCAGAATTCTGTAGTCCTAAAAGCAACTTCACGGGGACAGGGAAAATTGACAGCTTCATTGATTTACTTTAGTGCGCTAAATGAAACAAAGGAGGTTGTGCAAGAAGTCACTATATGTGATCAAGTGAAGTTTAGTTTGGACAAAAGTGTTGCGACCATTCTCCTTCCCTGGGTTCCAGCTGTTTATCAGGAGGTCGAGCTGAAGGTTTCAGGAGGTTGTGCAAAAGCATCTACAGACTACAAATGGTTCTCCTCAGACATGGGTATTGTGTCTGTATCTGCTTCTGGGGTTGTCCAGGCTAAGAAGCCTGGTAAAGCTACTATTAAAGTGCTATCCATTTTTGATTCATTCAATTATGATGAGGTGGTCATTGAAGTTTCTGTCCCAGCATCTATGTCCATGCTTCTTAACTTTCCTGTGGAGACTGTAGTAGGATCACATCTTCAAGCTGCTGTAACAATGAAAGCATCAAATGGTGCCTACTTTTATAGATGTGATGCATTCAGTTCCTTCATAAAGTGGAAAGTAGAGAGTGGGCCTTTCATCATTGTCAAAGGGGAGGCATCTGATTCACACATGTTAGGGAATGCTGAGTTCCATACATCAACTTATGGTTCTCCATGTTCATGGGCAGATTTATATGCTTCTGCTTCTGGTCGAGCTACTCTTCATGCTGCATTACCTAATGAATATCACAACTATGAAAGCTCTTTTCATGGACCTACTGTCTTAAAAGCATCATCACTTATTGCGGCATATCCACCACTTAGCGTTCGTCAGGCTGGTGATGGAAATCATTATGGTGGTTACTTTTTTGATTTGACTCTAACAGAAACTGATAATCCATCGGTAAAGTTGGATAAAATGTACCTTGTCCCTGGAACGCATCTGGATGTCATGCTTCTTGGTGGTCCCGAACAGTGGAAAAATGGTGTTGAGTTTGTGGAAACTAGGGACATCTTAAATAAAGAACATGGTCACATTGATGATGGTGCTTCTGTGCAACGGTTATCTGAGACCTATAGGAGTCTGTACAGAGTTTCATGCGAGATGCTTGGAACCTATAGTATTGTTTTCAAGCGTGGTAATCTTGTTGGGGATGACCATCCTGTGCCTGCAGTGGCTGACGTGTCTCTGTCTCTTATATGTAGCATTCCTACCTCAATTGCTTTGATAGCCGATGAACCTGTAAATCAACTTGAAGTTATAAGGACAGCAATTCAGGCGGACCGTAGTTCAGGGAGAATTCGTGTCACTCCCATTACTGTGGCAAATGAGCGCACCATTAGATTAGCTGCAGTTGGCATTACTAGTAGTGGAGAAGCTTTTGCAAACTCATCATCTCTTCATTTGCAGTGGGAACTTAGCAATTGTGATGGGATGGCCTATTGGGATGATGCTGATAATTTACAGAGATCAAAGTACAGTTGGGAAAAAATTTTGAGCCTGCAAAATGTGTCAGGCCTGTGTATTGTTCGTGCTACTGTTATTGGTTTCCACAATACCATGGTTCAACATAATACTATACCATTGCTTGAGAGTTCAGAGAATGTTCTTACAGATGCTATTCGTTTGCAGCTTGTTTCTACATTGAGGATTAGTCCAGAAAATCATTTGGTAGTTTTCAATCCCAATGCTAAGGTAAATCTGGCAATTTCTGGTGGGAGCTGTTTTCTGAAAGTTGTTGTAAATGATTCTCAAGTGGTAGAAGTCATTCAACCCCCAACGGATTTACAATGCTCACAACTGGTGCTGTCTCCTAAAGGTTTGGGGACTGCACTTGTGACAGTTTATGATATTGGGCTTGCTCCTCCACTTGCAGCTTCTGCTGTGGTACAAGTTGCAGAGATCGACTGGATAAAGATTATGTCACCAGAAGTTATAAGCCTTATGGAAGGGAGTTCACAGACCATTGATATAATGGCTGGTATTAGTGATGGGAGAACTTTTGACTCTTATCAGTTTGCCTACATTAGTGTTCAAGTGCATGTTGAGGATCAAATTATTGGGGTGTCAGATATTAATGACATCTCAAACACAGATGGTGGATATATAAATGTTCCAAAATTTAAGATTTTTGCTTCACATCTTGGGATCACAACTTTCTTTGTCAGTGCTTTGCAGCAATCTGGGCATGAAATTTTGAGTCAACCAATAATGGTGGAAGTCTATGCAGCACCAGAGGTTCACCCTCACGATATATTCCTTGTTCCTGGTGCATCTTATGTGCTTACTTTAAAGGGAGGCCCAACAGTTGGTGTAAATGTTGAATATACGAGTATGGATGATGAAGTAGCAACAATAGATGGATCTTCAGGACAATTATCTGCGAGATTACCTGGGAACACTACTATTCGTGCCAGAGTCTTAAAAAATGGAGATACTGTGATCTGTCAAGCATATACCAGTGTTAAAGTAGGAGTTCCTTCTTCGGTGATATTGAATGCACAAAGTGAACTGCTTGGTGTTGGCAAAGAGATGCCACTTTATCCTGTGTTTTCTGAGGGTGATCTGTTTTCTGTTTATGAGCAATGCCAAAATTACCATTGGAGCGGAGAAGATGAAAAGGTGTTGAGTTTTTATGGGTTAGAGCACTTGAATAGTGAAGAGTATGGATCTCAACAAGATTATGCAGAAAAATTTCGTTTTACAAGCCATATTAGTGAGGAGGAGCTTGGTTTTATCAAAGTAGTGCTTGGAAGATCTGCAGGGAGGACAAATATCGCAGTCTCATTCTCTTGTGAATTTGTATCTTCTGGTTCTAAGTCATGGAGAAGAATTTACAATGCCTCTGTATCAATATCGGTGGTGCCTGATCCCCCGCTTGCTCTTGGAGTTCCAATAACCTGGATTCTTCCTCCTCGTTATACTACAACTAGCCTTTTACCTTTATCTTCAGAATCACATGGCCAACGGGAGAGTCAGAGTCATAAAGGAACTATCATCTATTCTTTATTGAGAAATGTTCCCGACAAGAATGAAGTTCTGCAAAAAGATGCTATTTCCGTTGATGGAGACAGAATAAAGACATCAGAAAGTAACAATCTTGCATGCATTCAGGCAAAAGATCGAATGACAGGTAGAATTGAGATTGCTGCTTGTGTCAAAGTTGCTGAGGTGGCTCAAATAAGGATTTCAGATGACTGGTTACCATTTCGTGGGATCAATCTTGGTCTTGGTGCTGAGCTTTCTCTTCCAGTAGTCTATTTGGACGTACTAGGAAACCGTTTTTATGAAGCATATGACACCGTCCTCTTTGATGTTGAAACTGACAATCCTGATGTGGTGTCTCTAAACACGACGCTTGCTGGAAGTGGAATCATCCATCTCAAGGCAATGCGACATGGTAGAGCTCTTGTGCGAGTATCCATTGCTAGTATTCCCTTGAAGTCCGACTATATCCTGATATCAGTGGGAGCGCATATACATCCTCAGAACCCAGTTATTCACATAGGAAGCCATGTTAACTTCAGTATAGAAGGTTTAAATGATCAAATTTCTGGCCGGTGGCTTACTGCCAATCAAAGTGTCATATCTGTCTCCCCATTGTCTGGAGAAGCTGAAGTAATTGGGGAAGGTACAACTCAAGTACACTTTGAGGCGCCAAGCATGAAGTTGCGAACAACAGTGACAGTGCTAACAGATGCTATTGTTTCTGTTGAAGCTCCAAGGGAGACACTGACAAATGTTCCCTTCCCCACTAAAGGATATAACTTCTCTGTGAAGATCAGCGACAAGTATAAGGCATTTGGAAACACCAAGGGCTTCCAGTATGTTTGTAGAGTGGACCCTCCTTTTGTTGGGTATTCAAAGCCATGGATTGATCTTGATACTGGTAACTCATATTGCCTCTTCTTCCCTTACACACCGGAGCATTTGGTTCGTTTCAAGTCCAAGGATATGAAACCAGATATATCTGTTTCCATCAATGCTTCATTGAGAGGAGCTGATCATGTTTCAGGATCGGCATCTGCTCTTTTCGTTGGAGGTTTTTCTGTTATGGAGATGGGCAAGGATTCATTCCAGTTGAATTTGACTCCAGACTTTAACAAGACTATCATCACAATTCTGGGGAACACAGATGTTGAAATGTATTGGCGTGAGCGGGATTTATTACTCATTACTCCCATCCATAAAGAAGGTTTTGGGATCGATGGGCGTGCAAAGTATGAGGTCAAAATGTTAGGTACCAAGAGATTCAAAGATACAATTTTCATCACACTACCAGCCAATGGTCAAAGTGTAGAAATTGATGTCAATGGTGACCCTGGAGAAAAAGCAGCATCAGAAACTACCGCCATCAATTATACCCTTTGGGCAACCATGCTCGGAGGTCTTGCTCTACTGATTTTGATAGTGGTAGTCTTCAAGTATTATTCTGACAGACCAGATAGATCTCATATACCTGTTGCTCCTGCTACAAATTTTGCAGTCCCAGTCACACCTGAACGTAGCAGCCCTGCTGTTGTGAGTGAACTGTCTCCTCGAACACCTCAGCCCTTCATGGATTATGTGAGGAGGACGATAGATGAAACCCCGTACTACAAGCGAGAGCCACGAAGGAGGGTTAACCCACAGAATACTTTCTAG

Protein Analysis

1947

Amino Acids

213.03

Weight (kDa)

5.47

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_NUP210_1st PF22967 35 - 117 3.2e-17 NUP210 Ig-like domain 1
Ig_NUP210_2nd PF22969 137 - 232 1.5e-10 NUP210 Ig-like domain 2
Ig_NUP210_3rd PF22963 244 - 320 5.9e-06 NUP210 Ig-like domain 3
Ig_NUP210_5th PF26182 470 - 544 1.3e-06 NUP210 Ig-like domain 5
Big_2 PF02368 481 - 530 4.2e-06 Bacterial Ig-like domain (group 2)
Ig_NUP210_7th PF22962 705 - 802 2.1e-15 NUP210 Ig-like domain 7
Big_2 PF02368 1147 - 1214 1.7e-06 Bacterial Ig-like domain (group 2)
Ig_GP210_15th PF24425 1633 - 1746 2.9e-49 Nuclear pore complex protein GP210, Ig-like domain
Ig_GP210_16th PF24427 1758 - 1838 2.7e-36 Nuclear pore complex protein GP210, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10590 AT5G40480
fragaria_vesca FvH4_6g51430
malus_domestica MD17G1026700.v1.1
prunus_persica Prupe.3G292000_v2.0.a1 Prupe.3G292000_v2.0.a1
pyrus_communis pycom17g02180
rosa_chinensis RchiOBHm_Chr2g0173101
rosa_laevigata RLG00000022158
rosa_multiflora Rmu_sc0011236.1_g000011
rosa_roxburghii Rroxscaffold_2G00079240
rosa_rugosa Rorug02G0565200
rosa_samantha Rh2AG645300 Rh2BG656000 Rh2CG621100 Rh2DG669400
rosa_wichuraiana Rw2G053000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 786, 2462, 3137
AasI GACNNNNNNGTC 2 cut(s) 578, 1024
AatII GACGTC 1 cut(s) 102
Acc16I TGCGCA 1 cut(s) 1146
Acc65I GGTACC 1 cut(s) 5441
AccB1I GGYRCC 4 cut(s) 1730, 4063, 4855, 5441
AccB7I CCANNNNNTGG 4 cut(s) 3509, 4412, 4805, 5486
AccBSI CCGCTC 2 cut(s) 3807, 5359
AccII CGCG 1 cut(s) 250
AcoI YGGCCR 3 cut(s) 958, 4162, 4756
AcuI CTGAAG 7 cut(s) 134, 852, 1475, 3552, 4134, 4708, 4836
AcyI GRCGYC 3 cut(s) 62, 99, 4856
AdeI CACNNNGTG 1 cut(s) 3707
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 3 cut(s) 618, 1830, 2382
AhdI GACNNNNNGTC 1 cut(s) 1979
AhlI ACTAGT 2 cut(s) 227, 2564
AjiI CACGTC 1 cut(s) 2383
AjuI GAANNNNNNNTTGG 2 cut(s) 1373, 1405
Alw21I GWGCWC 3 cut(s) 77, 3849, 4609
AlwNI CAGNNNCTG 3 cut(s) 2090, 2378, 3080
AoxI GGCC 9 cut(s) 28, 958, 1136, 1790, 2637, 2716, 3499, 4162, 4756
ArsI GACNNNNNNTTYG 2 cut(s) 5033, 5065
AseI ATTAAT 1 cut(s) 3276
Asp700I GAANNNNTTC 4 cut(s) 1124, 2967, 3894, 5837
Asp718I GGTACC 1 cut(s) 5441
AspLEI GCGC 7 cut(s) 250, 773, 1147, 1330, 2535, 4675, 4858
AspS9I GGNCC 9 cut(s) 52, 83, 1136, 1790, 1973, 2165, 2482, 3500, 5041
AsuHPI GGTGA 9 cut(s) 503, 741, 1049, 2051, 3117, 3434, 3700, 3773, 5528
AvaII GGWCC 6 cut(s) 52, 83, 1973, 2165, 2482, 5041
BaeI ACNNNNGTAYC 4 cut(s) 693, 726, 936, 969
BalI TGGCCA 2 cut(s) 960, 4164
BanI GGYRCC 4 cut(s) 1730, 4063, 4855, 5441
BanII GRGCYC 2 cut(s) 77, 4609
BbsI GAAGAC 2 cut(s) 846, 5611
Bbv12I GWGCWC 3 cut(s) 77, 3849, 4609
BbvCI CCTCAGC 2 cut(s) 4368, 5748
BceAI ACGGC 2 cut(s) 75, 817
BcgI CGANNNNNNTGC 4 cut(s) 5117, 5151, 5396, 5430
BciVI GTATCC 1 cut(s) 4630
BclI TGATCA 3 cut(s) 1372, 4744, 5203
BcuI ACTAGT 2 cut(s) 227, 2564
BfaI CTAG 9 cut(s) 228, 488, 575, 2205, 2565, 4131, 4472, 4629, 5842
BfmI CTRYAG 9 cut(s) 717, 1263, 1485, 1680, 2275, 2317, 2370, 2550, 3960
BfoI RGCGCY 2 cut(s) 774, 4859
BfrI CTTAAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 4630
BglII AGATCT 2 cut(s) 3956, 5648
BlpI GCTNAGC 1 cut(s) 4437
Bme18I GGWCC 6 cut(s) 52, 83, 1973, 2165, 2482, 5041
BmeRI GACNNNNNGTC 1 cut(s) 1979
BmgBI CACGTC 1 cut(s) 2383
BmgT120I GGNCC 9 cut(s) 52, 83, 1136, 1790, 1973, 2165, 2482, 3500, 5041
BmiI GGNNCC 9 cut(s) 1137, 1428, 1732, 2167, 2314, 4065, 4857, 5043, 5443
BmrI ACTGGG 3 cut(s) 1189, 4688, 5684
BmuI ACTGGG 3 cut(s) 1189, 4688, 5684
BpiI GAAGAC 2 cut(s) 846, 5611
BpmI CTGGAG 4 cut(s) 339, 4827, 5277, 5544
Bpu10I CCTNAGC 4 cut(s) 916, 2892, 4368, 5748
Bpu1102I GCTNAGC 1 cut(s) 4437
BpuEI CTTGAG 6 cut(s) 695, 801, 2810, 2937, 4568, 4824
Bsa29I ATCGAT 1 cut(s) 5406
BsaBI GATNNNNATC 3 cut(s) 2046, 2358, 5221
BsaHI GRCGYC 3 cut(s) 62, 99, 4856
BsaI GGTCTC 2 cut(s) 564, 2264
BsaWI WCCGGW 2 cut(s) 133, 5122
BsaXI ACNNNNNCTCC 4 cut(s) 2488, 2518, 3624, 3654
Bse118I RCCGGY 1 cut(s) 4758
Bse3DI GCAATG 5 cut(s) 90, 2783, 3791, 4596, 4623
Bse8I GATNNNNATC 3 cut(s) 2046, 2358, 5221
BseCI ATCGAT 1 cut(s) 5406
BseJI GATNNNNATC 3 cut(s) 2046, 2358, 5221
BseMI GCAATG 5 cut(s) 90, 2783, 3791, 4596, 4623
BseRI GAGGAG 8 cut(s) 1495, 3054, 3935, 3938, 4104, 5211, 5727, 5785
BseYI CCCAGC 1 cut(s) 1639
BsgI GTGCAG 1 cut(s) 3015
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 9 cut(s) 30, 960, 1138, 1792, 2639, 2718, 3501, 4164, 4758
BshNI GGYRCC 4 cut(s) 1730, 4063, 4855, 5441
BshVI ATCGAT 1 cut(s) 5406
BsiHKAI GWGCWC 3 cut(s) 77, 3849, 4609
BsiSI CCGG 4 cut(s) 78, 134, 4759, 5123
BslFI GGGAC 8 cut(s) 820, 1302, 1622, 2114, 2151, 2222, 3040, 5672
BsmFI GGGAC 8 cut(s) 820, 1302, 1622, 2114, 2151, 2222, 3040, 5672
BsmI GAATGC 5 cut(s) 1754, 1846, 2406, 3703, 4308
BsnI GGCC 9 cut(s) 30, 960, 1138, 1792, 2639, 2718, 3501, 4164, 4758
Bso31I GGTCTC 2 cut(s) 564, 2264
Bsp1286I GDGCHC 3 cut(s) 77, 3849, 4609
Bsp1407I TGTACA 2 cut(s) 1045, 2286
Bsp1720I GCTNAGC 1 cut(s) 4437
Bsp19I CCATGG 2 cut(s) 2760, 5069
BspANI GGCC 9 cut(s) 30, 960, 1138, 1792, 2639, 2718, 3501, 4164, 4758
BspDI ATCGAT 1 cut(s) 5406
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 9 cut(s) 1137, 1428, 1732, 2167, 2314, 4065, 4857, 5043, 5443
BspMAI CTGCAG 3 cut(s) 2374, 2554, 3964
BspT107I GGYRCC 4 cut(s) 1730, 4063, 4855, 5441
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 2 cut(s) 564, 2264
BsrBI CCGCTC 2 cut(s) 3807, 5359
BsrDI GCAATG 5 cut(s) 90, 2783, 3791, 4596, 4623
BsrFI RCCGGY 1 cut(s) 4758
BsrGI TGTACA 2 cut(s) 1045, 2286
BssAI RCCGGY 1 cut(s) 4758
BssNI GRCGYC 3 cut(s) 62, 99, 4856
BssT1I CCWWGG 5 cut(s) 2760, 4922, 5013, 5069, 5148
Bst6I CTCTTC 7 cut(s) 420, 871, 1926, 2604, 3855, 4452, 5111
BstACI GRCGYC 3 cut(s) 62, 99, 4856
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 3080
BstAUI TGTACA 2 cut(s) 1045, 2286
BstC8I GCNNGC 9 cut(s) 408, 1253, 2370, 2702, 3060, 4080, 4306, 4560, 5415
BstDSI CCRYGG 2 cut(s) 2760, 5069
BstEII GGTNACC 2 cut(s) 4401, 5516
BstENI CCTNNNNNAGG 1 cut(s) 1047
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 2 cut(s) 774, 4859
BstHHI GCGC 7 cut(s) 250, 773, 1147, 1330, 2535, 4675, 4858
BstMWI GCNNNNNNNGC 8 cut(s) 165, 299, 1144, 2429, 2477, 3080, 3385, 4373
BstNSI RCATGY 4 cut(s) 622, 1834, 3245, 4308
BstPI GGTNACC 2 cut(s) 4401, 5516
BstSFI CTRYAG 9 cut(s) 717, 1263, 1485, 1680, 2275, 2317, 2370, 2550, 3960
BstUI CGCG 1 cut(s) 250
BstV2I GAAGAC 2 cut(s) 846, 5611
BstX2I RGATCY 4 cut(s) 3563, 3869, 3956, 5648
BstXI CCANNNNNNTGG 3 cut(s) 1881, 2521, 4101
BstYI RGATCY 4 cut(s) 3563, 3869, 3956, 5648
Bsu15I ATCGAT 1 cut(s) 5406
BsuI GTATCC 1 cut(s) 4630
BsuRI GGCC 9 cut(s) 30, 960, 1138, 1792, 2639, 2718, 3501, 4164, 4758
BsuTUI ATCGAT 1 cut(s) 5406
BtgI CCRYGG 2 cut(s) 2760, 5069
BtgZI GCGATG 1 cut(s) 174
BtrI CACGTC 1 cut(s) 2383
BtsI GCAGTG 4 cut(s) 232, 503, 2379, 2615
Cac8I GCNNGC 9 cut(s) 408, 1253, 2370, 2702, 3060, 4080, 4306, 4560, 5415
CaiI CAGNNNCTG 3 cut(s) 2090, 2378, 3080
CfoI GCGC 7 cut(s) 250, 773, 1147, 1330, 2535, 4675, 4858
Cfr10I RCCGGY 1 cut(s) 4758
Cfr13I GGNCC 9 cut(s) 52, 83, 1136, 1790, 1973, 2165, 2482, 3500, 5041
ClaI ATCGAT 1 cut(s) 5406
CpoI CGGWCCG 2 cut(s) 83, 2482
CseI GACGC 3 cut(s) 51, 136, 4564
CspCI CAANNNNNGTGG 6 cut(s) 461, 496, 3396, 3431, 4041, 4076
CspI CGGWCCG 2 cut(s) 83, 2482
DinI GGCGCC 1 cut(s) 4857
DraI TTTAAA 2 cut(s) 3492, 4740
DraIII CACNNNGTG 1 cut(s) 3707
DrdI GACNNNNNNGTC 2 cut(s) 578, 1024
DriI GACNNNNNGTC 1 cut(s) 1979
DseDI GACNNNNNNGTC 2 cut(s) 578, 1024
EaeI YGGCCR 3 cut(s) 958, 4162, 4756
Eam1104I CTCTTC 7 cut(s) 420, 871, 1926, 2604, 3855, 4452, 5111
Eam1105I GACNNNNNGTC 1 cut(s) 1979
EarI CTCTTC 7 cut(s) 420, 871, 1926, 2604, 3855, 4452, 5111
EciI GGCGGA 2 cut(s) 65, 2495
Ecl136II GAGCTC 2 cut(s) 75, 4607
Eco130I CCWWGG 5 cut(s) 2760, 4922, 5013, 5069, 5148
Eco147I AGGCCT 1 cut(s) 2718
Eco24I GRGCYC 2 cut(s) 77, 4609
Eco31I GGTCTC 2 cut(s) 564, 2264
Eco32I GATATC 1 cut(s) 4662
Eco47I GGWCC 6 cut(s) 52, 83, 1973, 2165, 2482, 5041
Eco53kI GAGCTC 2 cut(s) 75, 4607
Eco57I CTGAAG 7 cut(s) 134, 852, 1475, 3552, 4134, 4708, 4836
Eco91I GGTNACC 2 cut(s) 4401, 5516
EcoICRI GAGCTC 2 cut(s) 75, 4607
EcoNI CCTNNNNNAGG 1 cut(s) 1047
EcoO65I GGTNACC 2 cut(s) 4401, 5516
EcoRI GAATTC 2 cut(s) 1258, 2499
EcoRV GATATC 1 cut(s) 4662
EcoT14I CCWWGG 5 cut(s) 2760, 4922, 5013, 5069, 5148
EcoT22I ATGCAT 2 cut(s) 1756, 4310
EcoT38I GRGCYC 2 cut(s) 77, 4609
EgeI GGCGCC 1 cut(s) 4857
EheI GGCGCC 1 cut(s) 4857
ErhI CCWWGG 5 cut(s) 2760, 4922, 5013, 5069, 5148
FalI AAGNNNNNCTT 5 cut(s) 33, 380, 412, 3933, 3965
FaqI GGGAC 8 cut(s) 820, 1302, 1622, 2114, 2151, 2222, 3040, 5672
FauI CCCGC 2 cut(s) 4083, 5352
FauNDI CATATG 1 cut(s) 4495
FbaI TGATCA 3 cut(s) 1372, 4744, 5203
FriOI GRGCYC 2 cut(s) 77, 4609
FspBI CTAG 9 cut(s) 228, 488, 575, 2205, 2565, 4131, 4472, 4629, 5842
FspI TGCGCA 1 cut(s) 1146
GlaI GCGC 7 cut(s) 249, 772, 1146, 1329, 2534, 4674, 4857
GsaI CCCAGC 1 cut(s) 1643
GsuI CTGGAG 4 cut(s) 339, 4827, 5277, 5544
HaeII RGCGCY 2 cut(s) 774, 4859
HaeIII GGCC 9 cut(s) 30, 960, 1138, 1792, 2639, 2718, 3501, 4164, 4758
HapII CCGG 4 cut(s) 78, 134, 4759, 5123
HgaI GACGC 3 cut(s) 51, 136, 4564
HhaI GCGC 7 cut(s) 250, 773, 1147, 1330, 2535, 4675, 4858
Hin1I GRCGYC 3 cut(s) 62, 99, 4856
Hin6I GCGC 7 cut(s) 248, 771, 1145, 1328, 2533, 4673, 4856
HinP1I GCGC 7 cut(s) 248, 771, 1145, 1328, 2533, 4673, 4856
HincII GTYRAC 4 cut(s) 883, 3404, 4720, 5824
HindII GTYRAC 4 cut(s) 883, 3404, 4720, 5824
HindIII AAGCTT 3 cut(s) 10, 389, 2577
HpaI GTTAAC 2 cut(s) 4720, 5824
HpaII CCGG 4 cut(s) 78, 134, 4759, 5123
HphI GGTGA 9 cut(s) 503, 741, 1049, 2051, 3117, 3434, 3700, 3773, 5528
Hpy99I CGWCG 4 cut(s) 67, 101, 311, 4558
HpyCH4IV ACGT 5 cut(s) 99, 309, 2382, 4467, 5704
HpyF10VI GCNNNNNNNGC 8 cut(s) 165, 299, 1144, 2429, 2477, 3080, 3385, 4373
HpySE526I ACGT 5 cut(s) 99, 309, 2382, 4467, 5704
Hsp92I GRCGYC 3 cut(s) 62, 99, 4856
HspAI GCGC 7 cut(s) 248, 771, 1145, 1328, 2533, 4673, 4856
KasI GGCGCC 1 cut(s) 4855
KpnI GGTACC 1 cut(s) 5445
Ksp22I TGATCA 3 cut(s) 1372, 4744, 5203
KspAI GTTAAC 2 cut(s) 4720, 5824
MaeI CTAG 9 cut(s) 228, 488, 575, 2205, 2565, 4131, 4472, 4629, 5842
MaeII ACGT 5 cut(s) 99, 309, 2382, 4467, 5704
MbiI CCGCTC 2 cut(s) 3807, 5359
MfeI CAATTG 2 cut(s) 2418, 2623
MflI RGATCY 4 cut(s) 3563, 3869, 3956, 5648
MhlI GDGCHC 3 cut(s) 77, 3849, 4609
MlsI TGGCCA 2 cut(s) 960, 4164
MluNI TGGCCA 2 cut(s) 960, 4164
Mly113I GGCGCC 1 cut(s) 4856
MmeI TCCRAC 3 cut(s) 2091, 4671, 5218
Mox20I TGGCCA 2 cut(s) 960, 4164
Mph1103I ATGCAT 2 cut(s) 1756, 4310
MroXI GAANNNNTTC 4 cut(s) 1124, 2967, 3894, 5837
MscI TGGCCA 2 cut(s) 960, 4164
MslI CAYNNNNRTG 9 cut(s) 968, 1115, 1879, 1953, 2055, 2145, 2238, 2519, 3915
Msp20I TGGCCA 2 cut(s) 960, 4164
MspA1I CMGCKG 1 cut(s) 1433
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 4 cut(s) 78, 134, 4759, 5123
MunI CAATTG 2 cut(s) 2418, 2623
Mva1269I GAATGC 5 cut(s) 1754, 1846, 2406, 3703, 4308
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 8 cut(s) 165, 299, 1144, 2429, 2477, 3080, 3385, 4373
NarI GGCGCC 1 cut(s) 4856
NcoI CCATGG 2 cut(s) 2760, 5069
NdeI CATATG 1 cut(s) 4495
NlaIV GGNNCC 9 cut(s) 1137, 1428, 1732, 2167, 2314, 4065, 4857, 5043, 5443
NmeAIII GCCGAG 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 1146
NsiI ATGCAT 2 cut(s) 1756, 4310
NspI RCATGY 4 cut(s) 622, 1834, 3245, 4308
PaeI GCATGC 1 cut(s) 4308
PasI CCCWGGG 2 cut(s) 1132, 1422
PceI AGGCCT 1 cut(s) 2718
PciI ACATGT 2 cut(s) 618, 1830
PctI GAATGC 5 cut(s) 1754, 1846, 2406, 3703, 4308
PdmI GAANNNNTTC 4 cut(s) 1124, 2967, 3894, 5837
PflFI GACNNNGTC 1 cut(s) 4502
PflMI CCANNNNNTGG 4 cut(s) 3509, 4412, 4805, 5486
PluTI GGCGCC 1 cut(s) 4859
PscI ACATGT 2 cut(s) 618, 1830
PshBI ATTAAT 1 cut(s) 3276
PsiI TTATAA 3 cut(s) 786, 2462, 3137
Psp124BI GAGCTC 2 cut(s) 77, 4609
PspEI GGTNACC 2 cut(s) 4401, 5516
PspFI CCCAGC 1 cut(s) 1639
PspN4I GGNNCC 9 cut(s) 1137, 1428, 1732, 2167, 2314, 4065, 4857, 5043, 5443
PspPI GGNCC 9 cut(s) 52, 83, 1136, 1790, 1973, 2165, 2482, 3500, 5041
PstI CTGCAG 3 cut(s) 2374, 2554, 3964
PstNI CAGNNNCTG 3 cut(s) 2090, 2378, 3080
PsuI RGATCY 4 cut(s) 3563, 3869, 3956, 5648
PsyI GACNNNGTC 1 cut(s) 4502
PvuII CAGCTG 1 cut(s) 1433
RseI CAYNNNNRTG 9 cut(s) 968, 1115, 1879, 1953, 2055, 2145, 2238, 2519, 3915
Rsr2I CGGWCCG 2 cut(s) 83, 2482
RsrII CGGWCCG 2 cut(s) 83, 2482
SacI GAGCTC 2 cut(s) 77, 4609
Sau96I GGNCC 9 cut(s) 52, 83, 1136, 1790, 1973, 2165, 2482, 3500, 5041
SduI GDGCHC 3 cut(s) 77, 3849, 4609
SfcI CTRYAG 9 cut(s) 717, 1263, 1485, 1680, 2275, 2317, 2370, 2550, 3960
SfoI GGCGCC 1 cut(s) 4857
SinI GGWCC 6 cut(s) 52, 83, 1973, 2165, 2482, 5041
SmiMI CAYNNNNRTG 9 cut(s) 968, 1115, 1879, 1953, 2055, 2145, 2238, 2519, 3915
SmlI CTYRAG 7 cut(s) 530, 674, 816, 2789, 2952, 4583, 4839
SmoI CTYRAG 7 cut(s) 530, 674, 816, 2789, 2952, 4583, 4839
SpeI ACTAGT 2 cut(s) 227, 2564
SphI GCATGC 1 cut(s) 4308
SseBI AGGCCT 1 cut(s) 2718
SspDI GGCGCC 1 cut(s) 4855
SspMI CTAG 9 cut(s) 228, 488, 575, 2205, 2565, 4131, 4472, 4629, 5842
SstI GAGCTC 2 cut(s) 77, 4609
StuI AGGCCT 1 cut(s) 2718
StyI CCWWGG 5 cut(s) 2760, 4922, 5013, 5069, 5148
TaiI ACGT 5 cut(s) 102, 312, 2385, 4470, 5707
TaqI TCGA 7 cut(s) 639, 1449, 1912, 3105, 4324, 5406, 5740
TatI WGTACW 6 cut(s) 690, 754, 1045, 2286, 2676, 4843
TauI GCSGC 1 cut(s) 2009
TspGWI ACGGA 3 cut(s) 413, 2996, 4249
Tth111I GACNNNGTC 1 cut(s) 4502
Van91I CCANNNNNTGG 4 cut(s) 3509, 4412, 4805, 5486
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 6 cut(s) 52, 83, 1973, 2165, 2482, 5041
VspI ATTAAT 1 cut(s) 3276
XagI CCTNNNNNAGG 1 cut(s) 1047
XceI RCATGY 4 cut(s) 622, 1834, 3245, 4308
XcmI CCANNNNNNNNNTGG 2 cut(s) 846, 3172
XmnI GAANNNNTTC 4 cut(s) 1124, 2967, 3894, 5837
XspI CTAG 9 cut(s) 228, 488, 575, 2205, 2565, 4131, 4472, 4629, 5842
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 2 cut(s) 1756, 4310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.