Rw2G053000

Bacterial Ig-like domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014163
Physical Location & Seq
Forward (+)
80888401 .. 80900489
12089 bp
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UTR
Exon/CDS
Intron
Rw2G053000.1

Sequence Viewer

Length: 5856 bp
ATGTCGCCATGCTTCGCTTTTTTACTCGGCCTGGTTCTTTTGACTATGGCGGACCAAACGGCGTCGCATTTGAGCTCCGGCGGTCCGCACATTGCCGACGTCAATATCCTGCTGCCTCCGAAGATGACGCATCCGGTTGAGTATAGGCTTCAGGGAAGCGACGGCTGCTTCAAATGGTCATGGGATCATCACGATATTCTCTCTGTTCTGCCTGAATATTATTCAACTAGTCACTGCTCAACGAGTGCGCGGTTGAGATCAGTTGCTTCTTATAGTGGTAGGAAGGAGACTGCTGTTTATGCTACCGACGTAAATACTGGAGTTGTGATTCGGTGCAAAGTTTTTATTGACAAACTTTCCAGAATCCAGATATTTCATAATTCTGTTAAGCTTGACTTAGATGGGCTTGCCACTCTTCAAGTCCGTGCCTTTGATGATGAAGAAAATGTGTTTTCGTCACTGGTGGGGTTGCAATTTATGTGGCAGCTAGAAACCAATGAACCACTGCATCACCTTGTTCATGTTCCTCTTAAGGACTCGCCCCTGAGTGACTGTGGTGGATTGTGTGGAGACCTAGATGTCCAAATAAAACTTGAAGATAGTGGTGTGTTCTCAGACATGTATGTTGTAAAGGGAATCGAAATTGGGCATGAGATTGTTTCTGTGCATTTGCTTGAGCCACAATTCAAGTACATGGCTGATACGATTGTCCTAACTGTAGCAGAAGCCATGTCACTTGAACCTCCCTCACCTGTACTTGTCCTTGTTGGCGCTGCTGTTTGTTATAATCTAAAAGTTATTCGTGGGAACAAGGCTCAAGTGGTAACTTTGCCGTCCCCACATCATCAATGGTCTTCGTCAAACTCTTCAGTTGCTCATGTTGACTCTCTGACAGGCTTGACAAACGCATTGAGCTTAGGGGTAACAAATGTCATAGTTGAAGATACAAGGGTTACTGGCCACATACAAGTGTCCTCACTCAATGTTGTCCTGCCAGATTCTTTGTCTTTATACTTGACACCTCTGTCTGCTTCTGGTGATCCTGTACAAGGAACTAAAGCAATTCCATCCATGACCCGTTGGTATGGTGTTTCTGGTCATCAGTACCTCATTCAAATGAAGGTTTTCTCCCAGGGGCCAGATGCGCAAGAAATTTACATCACAGAGAATGATGATCTAAAGCTGTCCAAAACCCAGTCAGATTATTGGCAAATTTTTCCTGTATCTGATGATATTGCAGTCAAACGTAGCTGGCAGAATTCTGTAGTCCTAAAAGCAACTTCACGGGGACAGGGAAAATTGACAGCTTCATTGACTTACTTTAGTGCGCTAAATGAAACAAAGGAGGTTCTCGAGGTTGTGCAAGAAGTCACTATATGTGATCAAGTGAAGTTTTGTTTGGACAAAAGTGTTGCGACCATTCTCCTTCCCTGGGTTCCAGCTGTTTATCAGGTGGTCGAGCTGAAGGTTTCAGGAGGTTGTGCAAAAGCATCTACAGACTACAAATGGTTCTCCTCAGACATGGGTATTGTGTCTGTATCTGCTTCTGGGGTTGTCCAGGCTAAGAAGCCTGGTAAAGCTACTATTAAAGTGCTATCCATTTTTGATTCATTCAATTATGATGAGGTGGTCATTGAAGTTTCTGTCCCAGCATCTATGTCCATGCTTCTTAACTTTCCTGTGGAGACTGTAGTAGGATCACATCTTCAAGCTGCTGTAACAATGAAAGCATCAAATGGTGCCTACTTTTATAGATGTGATGCATTCAGTTCCTTCATAAAGTGGAAAGTAGAGAGTGGGCCTTTCATCATTGTCAAAGGGGAGGCATCTGATTCACACATGTTAGGGAATGCTGAGTTCCATACATCAACTTATGGTTCTCCATGTTCATGGGCAGATTTATATGCTTCTGCTTCTGGTCGAGCTACTCTTCATGCTGCATTACCTAATGAATATCACAACTATGAAAGCTCTTTTCATGGACCTACTGTCTTAAAAGCATCATCACTTATTGCGGCATATCCACCACTTAGCGTTCGTCAGGCTGGTGATGGAAATCATTATGGTGGTTACTTTTTTGATTTGACTCTAACAGAAACTGATAATCCATTGGTAAAGTTGGATAAAATGTACCTTGTCCCTGGAACGCATCTGGATGTCATGCTTCTTGGTGGTCCCGAACAGTGGAAAAATGGTGTTGAGTTTGTGGAAACTAGGGACATCTTAAATAAAGAACATGGTCACATTGATGATGGTGCTTTTGTGCAACGGTTATCTGAGACCTATAGGAGTCTGTACAGAGTTTCATGCGAGATGCTTGGAACCTATAGTATTGTTTTCAAGCGTGGTAATCTTGTTGGGGATGACCATCCTGTGCCTGCAGTGGCTGACGTGTCTCTGTCTCTTATATGTAGCATTCCTACCTCAATTGCTTTGATAGCCGATGAACCTGTCAATCAACTTGAAGTTATAAGGACAGCAATTCAGGCGGACCGTAGTTCAGGGAGAATTCGTGTCACTCCCATTACTGTGGCAAATGAGCGCACCATTAGATTAGCTGCAGTTGGCATTACTAGTAGTGGAGAAGCTTTTGCAAACTCATCATCTCTTCATTTGCAGTGGGAACTTAGCAATTGTGATGGGATGGCCTATTGGGATGATGCTGATAATTTACAGAGATCAAAGTTCAGTTGGGAAAAATTTTTGAGCCTGCAAAATGTGTCAGGCCTGTGTATTGTTCGTGCTACTGTTATTGGTTTCCACAATACCATGGTTCAACATAATACTAGACCATTGCTTGAGAGTTCAGAGAATGTTCTTAAAGATGCTATTCGTTTGCAGCTTGTTTCTACATTGAGGATTAGTCCAGAAAATCATTTGGTAGTTTTCAATCCCGATGCTAAGGTAAATCTGGCAATTTCTGGTGGGAGCTGTTTTCTGAAAGTTGTTGTAAATGATTCTCAAGTGGTAGAAGTCATTCAACCCCCAACGGATTTACAATGCTCACAACTGATGCTGTCTCCTAAAGGTTTGGGGACTGCACTTGTGACAGTTTATGATATTGGGCTTGCTCCTCCACTTGCAGCTTCTGCTGTGGTACAAGTTGCAGAGATCGACTGGATAAAGATTATGTCACCAGAAGTTATAAGCCTTATGGAAGGGAGTTCACAGACTATTGATATAATGGCTGGTATTAGTGATGGGAGAACTTTTGACTCTTATCAGTTTGCCTACATTAGTGTTCAAGTGCATGTTGAGGATCAAATTATTGAGGTGTCAGATATTAATGACATCTCAAACACAGATGGTGGATATATAAATGTTCCAAAATTTAAGATTTTTGCTTCACATCTTGGGATCACAACTTTCTTTGTCAGTGCTTTGCAGCAATCTGGGCATGAAATTTTGAGTCAACCAATAATGGTGGAAGTCTATGCAGCACCAGAGGTTCACCCTCACGATATATTCCTTGTTCCTGGTGCATCTTATGTGCTTACTTTAAAGGGAGGCCCAACAGTTGGTGTAAATGTTGAATATACGAGTATGGATGATGAAGTAGCAACAATAGATGGATCTTCAGGACAATTATCTGCGAGATTACCTGGGAACACTACTATTCGTGCCAGAGTCTTAAAAAATGGAGATACTGTGATCTGTCAAGCATATACCAGTGTTAAAGTAGGAGTTCCTTCTTCGGTGATATTGAATGCACAAAGTGAACTGCTTGGTGTTGGCAAAGAGATGCCACTTTATCCTGTGTTTTCTGAGGGTGATCTGTTTTCTGTTTATGAGCAATGCCAAAATTACCATTGGAGCGGAGAAGATGAAAAGGTGTTGAGTTTTTATGGATTAGAGCACTTGAATAGTGAAGAGTATGGATCTCAACAAGATTATGCAGAAAAATTTCGTTTTACAAGCCATATTAGTGAGGAGGAGCTTGGTTTTATCAAAGTAGTGCTTGGAAGATCTGCAGGGAGGACAAATATCGCAGTCTCATTCTCATGTGAATTTGTATCTTCTGGTTCTAAGTCATGGAGAAGAATTTACAATGCCTCTGTATCAATATCGGTGGTGCCTGATCCCCCGCTTGCTCTTGGAGTTCCAATAACCTGGATTCTTCCTCCTCATTATACTACAACTAGTCTTTTACCTTTATCTTCAGAATCACATGGCCAATGGGATAGTCAGAGTCATGAAGGAACTATCATCTATTCTTTATTGAGAAATGTTCCCGACAAGAATGAAGTTCTGCAAAAAGATGCTATTTCCATTGATGGAGACAGAATAAAGACATCAGAAAGTAACAATCTTGCTTGCATTCAGGCAAAAGATCGAATGACAGGTAGAATTGAGATTGCTGCTTGTGTCAAAGTTGCTGAGGTGGCTCAAATAAGGATTTCAGATGACTGGTTACCATTTCGTGGGATCAATCTTGGTCTTGGTGCTGAGCTTTCTCTTCCAGTAGTCTATTTGGACGTACTAGGAAACCGTTTTTATGAAGCATATGACACCGTCCTCTTTGATGTTGAAACTGACAATCCTGATGTGGTGTCTGTAAACACGACGCTTGCTGGAAGTGGAATCATCCATCTCAAGGCAATGCGACATGGTAGAGCTCTTGTGCGAGTATCCATTGCTAGTATTCCCTTGAAGTCCGACTATATCCTGATATCAGTGGGAGCGCATATACATCCTCAGAACCCAGTTATTCACATAGGAAGCCATGTTAACTTCAGTATAGAAGGTTTAAATGATCAAATTTCTGGCCGGTGGCTTACTGCCAATGAAAGTGTCATATCTGTCTCCCCATTGTCTGGAGAAGCTGAAGTAATTGGGGAAGGTACAACTCAAGTACACTTTGAGGCGCCAAGCATGAAGTTGCGAACAACAGTGACAGTGCTAACAGATGATATTGTTTCTGTTGAAGCTCCAAGGGAGACACTGACAAATGTTCCCTTCCCCACTAAAGGATATAACTTCTCTGTGAAGATCAGCGACAAGTATAAGGCATTTGGAAACACCAAGGGCTTCCAGTATGTTTGTAGAGTGGACCCTCCTTTTGTTGGGTATTCAAAGCCATGGATTGATCTTGATACTGGTAACTCATATTGCCTCTTCTTCCCTTACACACCGGAGCATTTGGTTCGTTTCAAGTCCAAGGATATGAAACCAGATATATCTGTTTCCATCAATGCTTCATTGAGAGGAGCTGATCATGTTTCAGGATCGGCATCTGCTCTTTTCGTTGGAGGTTTTTCTGTTATGGAGATGGGCAAGGATTCATTCCAGTTGAATTTGACTCCAGACTTTAACAAGACTATCATCACAATTCTGGGGAACACAGATGTTGAAATGTATTGGCGTGAGCGGGATTTATTACTCATTACTCCCATCCATAAAGAAGGTTTTGGGATCGATGGGCGTGCAAAGTATGAGGTCAAAATGTTAGGTACCAAGAGATTCAAAGATACAATTTTCATCACACTACCAGCCAATGGTCAAAGTGTAGAAATTGATGTCAATGGTGACCCTGGAGAAAAAGCAGCATCAGAAACTACCGCTATCAATTATACCCTTTGGGCAACCATGCTCGGAGGTCTTGCTCTACTGATTTTGATAGTGGTAGTCTTCAAGTATTATTCTGACAGGCCAGATAGATCTCATATACCTGTTGCTCCTGCTACTCCAAATTTTGCAGTCCCAGTCACACCTGAACGTAGCAGCCCTGCTGTTGTGAGTGAACTGTCTCCTCGAACACCTCAGCCCTTCATGGATTATGTGAGGAGGACGATAGATGAAACCCCGTACTACAAGCGAGAGCCACGAAGGAGGGTTAACCCACAGAATACTTTCTAG

Protein Analysis

1951

Amino Acids

213.87

Weight (kDa)

5.37

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_NUP210_1st PF22967 35 - 117 9e-17 NUP210 Ig-like domain 1
Ig_NUP210_2nd PF22969 137 - 232 1.5e-10 NUP210 Ig-like domain 2
Ig_NUP210_5th PF26182 473 - 547 2.8e-06 NUP210 Ig-like domain 5
Big_2 PF02368 486 - 533 8e-06 Bacterial Ig-like domain (group 2)
Ig_NUP210_7th PF22962 708 - 805 4.5e-15 NUP210 Ig-like domain 7
Big_2 PF02368 1150 - 1217 1.7e-06 Bacterial Ig-like domain (group 2)
Ig_GP210_15th PF24425 1636 - 1749 1.9e-49 Nuclear pore complex protein GP210, Ig-like domain
Ig_GP210_16th PF24427 1761 - 1841 2.7e-36 Nuclear pore complex protein GP210, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10590 AT5G40480
fragaria_vesca FvH4_6g51430
malus_domestica MD17G1026700.v1.1
prunus_persica Prupe.3G292000_v2.0.a1 Prupe.3G292000_v2.0.a1
pyrus_communis pycom17g02180
rosa_chinensis RchiOBHm_Chr2g0173101
rosa_laevigata RLG00000022158
rosa_multiflora Rmu_sc0011236.1_g000011
rosa_roxburghii Rroxscaffold_2G00079240
rosa_rugosa Rorug02G0565200
rosa_samantha Rh2AG645300 Rh2BG656000 Rh2CG621100 Rh2DG669400
rosa_wichuraiana Rw2G053000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 786, 2471, 3146
AasI GACNNNNNNGTC 2 cut(s) 578, 1024
AatII GACGTC 1 cut(s) 102
Acc16I TGCGCA 1 cut(s) 1146
Acc65I GGTACC 1 cut(s) 5450
AccB1I GGYRCC 4 cut(s) 1739, 4072, 4864, 5450
AccB7I CCANNNNNTGG 4 cut(s) 3518, 4421, 4814, 5495
AccBSI CCGCTC 2 cut(s) 3816, 5368
AccII CGCG 1 cut(s) 250
AcoI YGGCCR 3 cut(s) 958, 4171, 4765
AcuI CTGAAG 7 cut(s) 134, 852, 1484, 3561, 4143, 4717, 4845
AcyI GRCGYC 3 cut(s) 62, 99, 4865
AdeI CACNNNGTG 1 cut(s) 3716
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 3 cut(s) 618, 1839, 2391
AhdI GACNNNNNGTC 1 cut(s) 1988
AhlI ACTAGT 3 cut(s) 227, 2573, 4139
AjiI CACGTC 1 cut(s) 2392
AjuI GAANNNNNNNTTGG 2 cut(s) 1382, 1414
Alw21I GWGCWC 3 cut(s) 77, 3858, 4618
AlwNI CAGNNNCTG 3 cut(s) 2099, 2387, 3089
Ama87I CYCGRG 1 cut(s) 1352
ArsI GACNNNNNNTTYG 2 cut(s) 5042, 5074
AseI ATTAAT 1 cut(s) 3285
Asp700I GAANNNNTTC 4 cut(s) 1124, 2976, 3903, 5849
Asp718I GGTACC 1 cut(s) 5450
AspLEI GCGC 7 cut(s) 250, 773, 1147, 1330, 2544, 4684, 4867
AspS9I GGNCC 9 cut(s) 52, 83, 1136, 1799, 1982, 2174, 2491, 3509, 5050
AsuHPI GGTGA 9 cut(s) 503, 741, 1049, 2060, 3126, 3443, 3709, 3782, 5537
AvaI CYCGRG 1 cut(s) 1352
AvaII GGWCC 6 cut(s) 52, 83, 1982, 2174, 2491, 5050
BaeI ACNNNNGTAYC 4 cut(s) 693, 726, 936, 969
BalI TGGCCA 2 cut(s) 960, 4173
BanI GGYRCC 4 cut(s) 1739, 4072, 4864, 5450
BanII GRGCYC 2 cut(s) 77, 4618
BbsI GAAGAC 2 cut(s) 846, 5620
Bbv12I GWGCWC 3 cut(s) 77, 3858, 4618
BbvCI CCTCAGC 2 cut(s) 4377, 5760
BceAI ACGGC 3 cut(s) 75, 178, 817
BcgI CGANNNNNNTGC 4 cut(s) 5126, 5160, 5405, 5439
BciVI GTATCC 1 cut(s) 4639
BclI TGATCA 3 cut(s) 1381, 4753, 5212
BcuI ACTAGT 3 cut(s) 227, 2573, 4139
BfmI CTRYAG 9 cut(s) 717, 1263, 1494, 1689, 2284, 2326, 2379, 2559, 3969
BfoI RGCGCY 2 cut(s) 774, 4868
BfrI CTTAAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 4639
BglII AGATCT 2 cut(s) 3965, 5657
BlpI GCTNAGC 1 cut(s) 4446
Bme18I GGWCC 6 cut(s) 52, 83, 1982, 2174, 2491, 5050
BmeRI GACNNNNNGTC 1 cut(s) 1988
BmeT110I CYCGRG 1 cut(s) 1352
BmgBI CACGTC 1 cut(s) 2392
BmgT120I GGNCC 9 cut(s) 52, 83, 1136, 1799, 1982, 2174, 2491, 3509, 5050
BmiI GGNNCC 9 cut(s) 1137, 1437, 1741, 2176, 2323, 4074, 4866, 5052, 5452
BmrI ACTGGG 3 cut(s) 1189, 4697, 5696
BmuI ACTGGG 3 cut(s) 1189, 4697, 5696
BpiI GAAGAC 2 cut(s) 846, 5620
BpmI CTGGAG 4 cut(s) 339, 4836, 5286, 5553
Bpu10I CCTNAGC 4 cut(s) 916, 2901, 4377, 5760
Bpu1102I GCTNAGC 1 cut(s) 4446
BpuEI CTTGAG 6 cut(s) 695, 801, 2819, 2946, 4577, 4833
Bsa29I ATCGAT 1 cut(s) 5415
BsaBI GATNNNNATC 3 cut(s) 2055, 2367, 5230
BsaHI GRCGYC 3 cut(s) 62, 99, 4865
BsaI GGTCTC 2 cut(s) 564, 2273
BsaWI WCCGGW 2 cut(s) 133, 5131
BsaXI ACNNNNNCTCC 4 cut(s) 2497, 2527, 3633, 3663
Bse118I RCCGGY 1 cut(s) 4767
Bse3DI GCAATG 5 cut(s) 90, 2792, 3800, 4605, 4632
Bse8I GATNNNNATC 3 cut(s) 2055, 2367, 5230
BseCI ATCGAT 1 cut(s) 5415
BseJI GATNNNNATC 3 cut(s) 2055, 2367, 5230
BseMI GCAATG 5 cut(s) 90, 2792, 3800, 4605, 4632
BseRI GAGGAG 8 cut(s) 1504, 3063, 3944, 3947, 4113, 5220, 5739, 5797
BseYI CCCAGC 1 cut(s) 1648
BsgI GTGCAG 1 cut(s) 3024
Bsh1236I CGCG 1 cut(s) 250
BshNI GGYRCC 4 cut(s) 1739, 4072, 4864, 5450
BshVI ATCGAT 1 cut(s) 5415
BsiHKAI GWGCWC 3 cut(s) 77, 3858, 4618
BsiHKCI CYCGRG 1 cut(s) 1352
BsiSI CCGG 4 cut(s) 78, 134, 4768, 5132
BslFI GGGAC 8 cut(s) 820, 1302, 1631, 2123, 2160, 2231, 3049, 5684
BsmFI GGGAC 8 cut(s) 820, 1302, 1631, 2123, 2160, 2231, 3049, 5684
BsmI GAATGC 5 cut(s) 1763, 1855, 2415, 3712, 4317
Bso31I GGTCTC 2 cut(s) 564, 2273
BsoBI CYCGRG 1 cut(s) 1352
Bsp1286I GDGCHC 3 cut(s) 77, 3858, 4618
Bsp1407I TGTACA 2 cut(s) 1045, 2295
Bsp1720I GCTNAGC 1 cut(s) 4446
Bsp19I CCATGG 2 cut(s) 2769, 5078
BspDI ATCGAT 1 cut(s) 5415
BspFNI CGCG 1 cut(s) 250
BspHI TCATGA 1 cut(s) 4192
BspLI GGNNCC 9 cut(s) 1137, 1437, 1741, 2176, 2323, 4074, 4866, 5052, 5452
BspMAI CTGCAG 3 cut(s) 2383, 2563, 3973
BspT107I GGYRCC 4 cut(s) 1739, 4072, 4864, 5450
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 2 cut(s) 564, 2273
BsrBI CCGCTC 2 cut(s) 3816, 5368
BsrDI GCAATG 5 cut(s) 90, 2792, 3800, 4605, 4632
BsrFI RCCGGY 1 cut(s) 4767
BsrGI TGTACA 2 cut(s) 1045, 2295
BssAI RCCGGY 1 cut(s) 4767
BssNI GRCGYC 3 cut(s) 62, 99, 4865
BssT1I CCWWGG 5 cut(s) 2769, 4931, 5022, 5078, 5157
Bst6I CTCTTC 7 cut(s) 420, 871, 1935, 2613, 3864, 4461, 5120
BstACI GRCGYC 3 cut(s) 62, 99, 4865
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 3089
BstAUI TGTACA 2 cut(s) 1045, 2295
BstC8I GCNNGC 9 cut(s) 408, 1253, 2379, 2711, 3069, 4089, 4315, 4569, 5424
BstDSI CCRYGG 2 cut(s) 2769, 5078
BstEII GGTNACC 2 cut(s) 4410, 5525
BstENI CCTNNNNNAGG 1 cut(s) 1047
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 2 cut(s) 774, 4868
BstHHI GCGC 7 cut(s) 250, 773, 1147, 1330, 2544, 4684, 4867
BstMWI GCNNNNNNNGC 8 cut(s) 165, 299, 1144, 2438, 2486, 3089, 3394, 4382
BstNSI RCATGY 3 cut(s) 622, 1843, 3254
BstPI GGTNACC 2 cut(s) 4410, 5525
BstSFI CTRYAG 9 cut(s) 717, 1263, 1494, 1689, 2284, 2326, 2379, 2559, 3969
BstUI CGCG 1 cut(s) 250
BstV2I GAAGAC 2 cut(s) 846, 5620
BstX2I RGATCY 4 cut(s) 3572, 3878, 3965, 5657
BstXI CCANNNNNNTGG 3 cut(s) 1890, 2530, 4110
BstYI RGATCY 4 cut(s) 3572, 3878, 3965, 5657
Bsu15I ATCGAT 1 cut(s) 5415
BsuI GTATCC 1 cut(s) 4639
BsuTUI ATCGAT 1 cut(s) 5415
BtgI CCRYGG 2 cut(s) 2769, 5078
BtrI CACGTC 1 cut(s) 2392
BtsI GCAGTG 4 cut(s) 232, 503, 2388, 2624
Cac8I GCNNGC 9 cut(s) 408, 1253, 2379, 2711, 3069, 4089, 4315, 4569, 5424
CaiI CAGNNNCTG 3 cut(s) 2099, 2387, 3089
CciI TCATGA 1 cut(s) 4192
CfoI GCGC 7 cut(s) 250, 773, 1147, 1330, 2544, 4684, 4867
Cfr10I RCCGGY 1 cut(s) 4767
Cfr13I GGNCC 9 cut(s) 52, 83, 1136, 1799, 1982, 2174, 2491, 3509, 5050
ClaI ATCGAT 1 cut(s) 5415
CpoI CGGWCCG 2 cut(s) 83, 2491
CseI GACGC 3 cut(s) 51, 136, 4573
CspCI CAANNNNNGTGG 6 cut(s) 461, 496, 3405, 3440, 4050, 4085
CspI CGGWCCG 2 cut(s) 83, 2491
DinI GGCGCC 1 cut(s) 4866
DraI TTTAAA 2 cut(s) 3501, 4749
DraIII CACNNNGTG 1 cut(s) 3716
DrdI GACNNNNNNGTC 2 cut(s) 578, 1024
DriI GACNNNNNGTC 1 cut(s) 1988
DseDI GACNNNNNNGTC 2 cut(s) 578, 1024
EaeI YGGCCR 3 cut(s) 958, 4171, 4765
Eam1104I CTCTTC 7 cut(s) 420, 871, 1935, 2613, 3864, 4461, 5120
Eam1105I GACNNNNNGTC 1 cut(s) 1988
EarI CTCTTC 7 cut(s) 420, 871, 1935, 2613, 3864, 4461, 5120
EciI GGCGGA 2 cut(s) 65, 2504
Ecl136II GAGCTC 2 cut(s) 75, 4616
Eco130I CCWWGG 5 cut(s) 2769, 4931, 5022, 5078, 5157
Eco147I AGGCCT 1 cut(s) 2727
Eco24I GRGCYC 2 cut(s) 77, 4618
Eco31I GGTCTC 2 cut(s) 564, 2273
Eco32I GATATC 1 cut(s) 4671
Eco47I GGWCC 6 cut(s) 52, 83, 1982, 2174, 2491, 5050
Eco53kI GAGCTC 2 cut(s) 75, 4616
Eco57I CTGAAG 7 cut(s) 134, 852, 1484, 3561, 4143, 4717, 4845
Eco88I CYCGRG 1 cut(s) 1352
Eco91I GGTNACC 2 cut(s) 4410, 5525
EcoICRI GAGCTC 2 cut(s) 75, 4616
EcoNI CCTNNNNNAGG 1 cut(s) 1047
EcoO65I GGTNACC 2 cut(s) 4410, 5525
EcoRI GAATTC 2 cut(s) 1258, 2508
EcoRV GATATC 1 cut(s) 4671
EcoT14I CCWWGG 5 cut(s) 2769, 4931, 5022, 5078, 5157
EcoT22I ATGCAT 1 cut(s) 1765
EcoT38I GRGCYC 2 cut(s) 77, 4618
EgeI GGCGCC 1 cut(s) 4866
EheI GGCGCC 1 cut(s) 4866
ErhI CCWWGG 5 cut(s) 2769, 4931, 5022, 5078, 5157
FalI AAGNNNNNCTT 4 cut(s) 380, 412, 3942, 3974
FaqI GGGAC 8 cut(s) 820, 1302, 1631, 2123, 2160, 2231, 3049, 5684
FauI CCCGC 2 cut(s) 4092, 5361
FauNDI CATATG 1 cut(s) 4504
FbaI TGATCA 3 cut(s) 1381, 4753, 5212
FriOI GRGCYC 2 cut(s) 77, 4618
FspI TGCGCA 1 cut(s) 1146
GlaI GCGC 7 cut(s) 249, 772, 1146, 1329, 2543, 4683, 4866
GsaI CCCAGC 1 cut(s) 1652
GsuI CTGGAG 4 cut(s) 339, 4836, 5286, 5553
HaeII RGCGCY 2 cut(s) 774, 4868
HapII CCGG 4 cut(s) 78, 134, 4768, 5132
HgaI GACGC 3 cut(s) 51, 136, 4573
HhaI GCGC 7 cut(s) 250, 773, 1147, 1330, 2544, 4684, 4867
Hin1I GRCGYC 3 cut(s) 62, 99, 4865
Hin6I GCGC 7 cut(s) 248, 771, 1145, 1328, 2542, 4682, 4865
HinP1I GCGC 7 cut(s) 248, 771, 1145, 1328, 2542, 4682, 4865
HincII GTYRAC 4 cut(s) 883, 3413, 4729, 5836
HindII GTYRAC 4 cut(s) 883, 3413, 4729, 5836
HindIII AAGCTT 2 cut(s) 389, 2586
HpaI GTTAAC 2 cut(s) 4729, 5836
HpaII CCGG 4 cut(s) 78, 134, 4768, 5132
HphI GGTGA 9 cut(s) 503, 741, 1049, 2060, 3126, 3443, 3709, 3782, 5537
Hpy99I CGWCG 5 cut(s) 67, 101, 164, 311, 4567
HpyCH4IV ACGT 6 cut(s) 99, 309, 1246, 2391, 4476, 5716
HpyF10VI GCNNNNNNNGC 8 cut(s) 165, 299, 1144, 2438, 2486, 3089, 3394, 4382
HpySE526I ACGT 6 cut(s) 99, 309, 1246, 2391, 4476, 5716
Hsp92I GRCGYC 3 cut(s) 62, 99, 4865
HspAI GCGC 7 cut(s) 248, 771, 1145, 1328, 2542, 4682, 4865
KasI GGCGCC 1 cut(s) 4864
KpnI GGTACC 1 cut(s) 5454
Ksp22I TGATCA 3 cut(s) 1381, 4753, 5212
KspAI GTTAAC 2 cut(s) 4729, 5836
MaeII ACGT 6 cut(s) 99, 309, 1246, 2391, 4476, 5716
MbiI CCGCTC 2 cut(s) 3816, 5368
MfeI CAATTG 2 cut(s) 2427, 2632
MflI RGATCY 4 cut(s) 3572, 3878, 3965, 5657
MhlI GDGCHC 3 cut(s) 77, 3858, 4618
MlsI TGGCCA 2 cut(s) 960, 4173
MluNI TGGCCA 2 cut(s) 960, 4173
Mly113I GGCGCC 1 cut(s) 4865
MlyI GAGTC 9 cut(s) 530, 878, 2080, 2299, 3209, 3418, 3636, 4198, 5293
MmeI TCCRAC 3 cut(s) 2100, 4680, 5227
Mox20I TGGCCA 2 cut(s) 960, 4173
Mph1103I ATGCAT 1 cut(s) 1765
MroXI GAANNNNTTC 4 cut(s) 1124, 2976, 3903, 5849
MscI TGGCCA 2 cut(s) 960, 4173
Msp20I TGGCCA 2 cut(s) 960, 4173
MspA1I CMGCKG 1 cut(s) 1442
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 4 cut(s) 78, 134, 4768, 5132
MunI CAATTG 2 cut(s) 2427, 2632
Mva1269I GAATGC 5 cut(s) 1763, 1855, 2415, 3712, 4317
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 8 cut(s) 165, 299, 1144, 2438, 2486, 3089, 3394, 4382
NarI GGCGCC 1 cut(s) 4865
NcoI CCATGG 2 cut(s) 2769, 5078
NdeI CATATG 1 cut(s) 4504
NlaIV GGNNCC 9 cut(s) 1137, 1437, 1741, 2176, 2323, 4074, 4866, 5052, 5452
NmeAIII GCCGAG 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 1146
NsiI ATGCAT 1 cut(s) 1765
NspI RCATGY 3 cut(s) 622, 1843, 3254
PaeR7I CTCGAG 1 cut(s) 1352
PagI TCATGA 1 cut(s) 4192
PasI CCCWGGG 2 cut(s) 1132, 1431
PceI AGGCCT 1 cut(s) 2727
PciI ACATGT 2 cut(s) 618, 1839
PctI GAATGC 5 cut(s) 1763, 1855, 2415, 3712, 4317
PdmI GAANNNNTTC 4 cut(s) 1124, 2976, 3903, 5849
PflFI GACNNNGTC 1 cut(s) 4511
PflMI CCANNNNNTGG 4 cut(s) 3518, 4421, 4814, 5495
PleI GAGTC 9 cut(s) 530, 878, 2080, 2298, 3209, 3417, 3635, 4197, 5293
PluTI GGCGCC 1 cut(s) 4868
PpsI GAGTC 9 cut(s) 530, 878, 2080, 2298, 3209, 3417, 3635, 4197, 5293
PscI ACATGT 2 cut(s) 618, 1839
PshBI ATTAAT 1 cut(s) 3285
PsiI TTATAA 3 cut(s) 786, 2471, 3146
Psp124BI GAGCTC 2 cut(s) 77, 4618
PspEI GGTNACC 2 cut(s) 4410, 5525
PspFI CCCAGC 1 cut(s) 1648
PspN4I GGNNCC 9 cut(s) 1137, 1437, 1741, 2176, 2323, 4074, 4866, 5052, 5452
PspPI GGNCC 9 cut(s) 52, 83, 1136, 1799, 1982, 2174, 2491, 3509, 5050
PstI CTGCAG 3 cut(s) 2383, 2563, 3973
PstNI CAGNNNCTG 3 cut(s) 2099, 2387, 3089
PsuI RGATCY 4 cut(s) 3572, 3878, 3965, 5657
PsyI GACNNNGTC 1 cut(s) 4511
PvuII CAGCTG 1 cut(s) 1442
Rsr2I CGGWCCG 2 cut(s) 83, 2491
RsrII CGGWCCG 2 cut(s) 83, 2491
SacI GAGCTC 2 cut(s) 77, 4618
Sau96I GGNCC 9 cut(s) 52, 83, 1136, 1799, 1982, 2174, 2491, 3509, 5050
SchI GAGTC 9 cut(s) 530, 878, 2080, 2299, 3209, 3418, 3636, 4198, 5293
SduI GDGCHC 3 cut(s) 77, 3858, 4618
SfcI CTRYAG 9 cut(s) 717, 1263, 1494, 1689, 2284, 2326, 2379, 2559, 3969
SfoI GGCGCC 1 cut(s) 4866
Sfr274I CTCGAG 1 cut(s) 1352
SinI GGWCC 6 cut(s) 52, 83, 1982, 2174, 2491, 5050
SlaI CTCGAG 1 cut(s) 1352
SmlI CTYRAG 8 cut(s) 530, 674, 816, 1352, 2798, 2961, 4592, 4848
SmoI CTYRAG 8 cut(s) 530, 674, 816, 1352, 2798, 2961, 4592, 4848
SpeI ACTAGT 3 cut(s) 227, 2573, 4139
SseBI AGGCCT 1 cut(s) 2727
SspDI GGCGCC 1 cut(s) 4864
SspI AATATT 1 cut(s) 218
SstI GAGCTC 2 cut(s) 77, 4618
StuI AGGCCT 1 cut(s) 2727
StyI CCWWGG 5 cut(s) 2769, 4931, 5022, 5078, 5157
TaiI ACGT 6 cut(s) 102, 312, 1249, 2394, 4479, 5719
TaqI TCGA 8 cut(s) 639, 1353, 1458, 1921, 3114, 4333, 5415, 5752
TatI WGTACW 5 cut(s) 690, 754, 1045, 2295, 4852
TauI GCSGC 1 cut(s) 2018
TspGWI ACGGA 2 cut(s) 413, 3005
Tth111I GACNNNGTC 1 cut(s) 4511
Van91I CCANNNNNTGG 4 cut(s) 3518, 4421, 4814, 5495
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 6 cut(s) 52, 83, 1982, 2174, 2491, 5050
VspI ATTAAT 1 cut(s) 3285
XagI CCTNNNNNAGG 1 cut(s) 1047
XceI RCATGY 3 cut(s) 622, 1843, 3254
XcmI CCANNNNNNNNNTGG 1 cut(s) 846
XhoI CTCGAG 1 cut(s) 1352
XmnI GAANNNNTTC 4 cut(s) 1124, 2976, 3903, 5849
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.