AT5G43940

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013367
Physical Location & Seq
Forward (+)
17684156 .. 17686534
2379 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43940.2

Sequence Viewer

Length: 1140 bp
ATGGCGACTCAAGGTCAGGTTATCACTTGCAAAGCTGCGGTGGCTTACGAGCCGAACAAGCCTCTGGTCATCGAAGATGTGCAAGTGGCTCCACCTCAAGCTGGTGAGGTTCGGATCAAGATCCTCTACACTGCTCTTTGTCACACCGACGCTTACACTTGGAGCGGCAAGGATCCTGAAGGTCTCTTTCCTTGTATTCTAGGTCATGAGGCTGCTGGGATTGTTGAGAGTGTTGGTGAAGGAGTAACTGAAGTTCAAGCTGGAGATCATGTTATCCCTTGTTATCAAGCTGAGTGTCGTGAATGCAAGTTTTGCAAATCTGGGAAGACTAACCTTTGTGGCAAGGTGAGATCTGCTACTGGTGTTGGGATTATGATGAATGACCGTAAGTCGAGGTTCTCGGTTAATGGGAAACCCATTTATCACTTCATGGGTACTTCCACGTTTAGTCAGTATACTGTTGTTCATGATGTTAGCGTCGCCAAAATTGATCCTACTGCTCCTTTGGATAAAGTTTGCCTTCTTGGATGTGGTGTTCCCACTGGCCTTGGAGCAGTTTGGAATACTGCAAAAGTAGAACCAGGGTCAAATGTTGCCATTTTCGGTCTTGGCACTGTTGGACTTGCTGTTGCCGAGGGTGCGAAAACAGCTGGTGCTTCAAGGATCATTGGCATTGACATCGATAGCAAGAAGTATGAAACTGCAAAGAAGTTTGGTGTTAACGAATTTGTGAACCCAAAGGATCACGACAAGCCAATTCAGGAAGTGATTGTCGATCTCACTGATGGCGGTGTTGACTACAGCTTTGAGTGCATCGGGAATGTCTCCGTGATGAGAGCTGCATTGGAGTGCTGTCACAAGGGATGGGGAACTTCGGTCATAGTTGGTGTTGCAGCATCAGGACAAGAGATATCAACTCGTCCGTTCCAACTCGTGACTGGCCGTGTGTGGAAAGGAACAGCTTTTGGTGGTTTCAAGAGTCGAACCCAAGTGCCTTGGCTTGTAGAAAAGTACATGAACAAGGAGATAAAAGTGGATGAATACATAACACACAACTTGACCTTGGGAGAAATCAATAAGGCTTTCGATCTATTGCACGAAGGTACTTGCCTTCGTTGTGTCCTCGATACCAGCAAATGA

Protein Analysis

379

Amino Acids

40.7

Weight (kDa)

6.51

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 46 - 175 1.3e-22 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 218 - 341 5.7e-26 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43940 AT5G43940
fragaria_vesca FvH4_2g20240
malus_domestica MD05G1186000.v1.1 MD10G1174100.v1.1
prunus_persica Prupe.8G212700_v2.0.a1
pyrus_communis pycom05g17060 pycom10g15010
rosa_chinensis RchiOBHm_Chr6g0286011
rosa_laevigata RLG00000012590
rosa_multiflora Rmu_sc0019472.1_g000001
rosa_roxburghii Rroxscaffold_7G00182530
rosa_rugosa Rorug06G0178200
rosa_samantha Rh6AG290200 Rh6BG292200 Rh6CG293200 Rh6DG285300
rosa_wichuraiana Rw6G025010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 165
AccI GTMKAC 1 cut(s) 455
AciI CCGC 3 cut(s) 38, 165, 789
AclWI GGATC 7 cut(s) 115, 122, 167, 180, 485, 671, 750
AcoI YGGCCR 1 cut(s) 940
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 2 cut(s) 198, 270
AfaI GTAC 3 cut(s) 436, 1013, 1105
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 3 cut(s) 257, 660, 976
AhdI GACNNNNNGTC 2 cut(s) 12, 388
AjnI CCWGG 1 cut(s) 580
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 8 cut(s) 35, 101, 260, 290, 650, 804, 839, 962
AluI AGCT 8 cut(s) 35, 101, 260, 290, 650, 804, 839, 962
Alw26I GTCTC 2 cut(s) 188, 829
AlwI GGATC 7 cut(s) 115, 122, 167, 180, 485, 671, 750
AoxI GGCC 2 cut(s) 544, 940
ApeKI GCWGC 4 cut(s) 35, 212, 839, 893
ApoI RAATTY 1 cut(s) 725
ArsI GACNNNNNNTTYG 2 cut(s) 788, 820
AsuHPI GGTGA 3 cut(s) 116, 248, 358
BamHI GGATCC 1 cut(s) 172
BauI CACGAG 1 cut(s) 932
BbsI GAAGAC 1 cut(s) 332
BbvI GCAGC 4 cut(s) 22, 199, 826, 905
BccI CCATC 2 cut(s) 779, 858
BceAI ACGGC 1 cut(s) 927
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BciT130I CCWGG 1 cut(s) 582
BcoDI GTCTC 2 cut(s) 188, 829
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 799
BglII AGATCT 1 cut(s) 350
BisI GCNGC 5 cut(s) 36, 166, 213, 840, 894
BlsI GCNGC 5 cut(s) 37, 167, 214, 841, 895
Bme1390I CCNGG 1 cut(s) 582
BmeRI GACNNNNNGTC 2 cut(s) 12, 388
BmiI GGNNCC 2 cut(s) 90, 174
BmrFI CCNGG 1 cut(s) 582
BmsI GCATC 2 cut(s) 822, 905
BpiI GAAGAC 1 cut(s) 332
BpmI CTGGAG 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 81
Bsa29I ATCGAT 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 5 cut(s) 547, 581, 633, 995, 1062
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 3 cut(s) 364, 547, 943
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 1 cut(s) 582
BseCI ATCGAT 1 cut(s) 681
BseDI CCNNGG 5 cut(s) 547, 581, 633, 995, 1062
BseGI GGATG 3 cut(s) 533, 869, 1042
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 3 cut(s) 364, 547, 943
BseXI GCAGC 4 cut(s) 22, 199, 826, 905
BseYI CCCAGC 1 cut(s) 215
BshFI GGCC 2 cut(s) 546, 942
BshVI ATCGAT 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 2 cut(s) 188, 829
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 2 cut(s) 546, 942
Bso31I GGTCTC 1 cut(s) 188
BspACI CCGC 3 cut(s) 38, 165, 789
BspANI GGCC 2 cut(s) 546, 942
BspCNI CTCAG 1 cut(s) 283
BspDI ATCGAT 1 cut(s) 681
BspHI TCATGA 2 cut(s) 205, 466
BspLI GGNNCC 2 cut(s) 90, 174
BspPI GGATC 7 cut(s) 115, 122, 167, 180, 485, 671, 750
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 165
BsrI ACTGG 3 cut(s) 364, 547, 943
BssECI CCNNGG 5 cut(s) 547, 581, 633, 995, 1062
BssNAI GTATAC 1 cut(s) 456
BssSI CACGAG 1 cut(s) 932
BssT1I CCWWGG 3 cut(s) 547, 995, 1062
Bst1107I GTATAC 1 cut(s) 456
Bst2BI CACGAG 1 cut(s) 932
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 3 cut(s) 386, 460, 616
BstAPI GCANNNNNTGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 3 cut(s) 533, 869, 1042
BstMAI GTCTC 2 cut(s) 188, 829
BstMWI GCNNNNNNNGC 6 cut(s) 41, 58, 312, 638, 647, 810
BstNI CCWGG 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 580
BstSFI CTRYAG 1 cut(s) 799
BstV1I GCAGC 4 cut(s) 22, 199, 826, 905
BstV2I GAAGAC 1 cut(s) 332
BstX2I RGATCY 3 cut(s) 120, 172, 350
BstYI RGATCY 3 cut(s) 120, 172, 350
BstZ17I GTATAC 1 cut(s) 456
Bsu15I ATCGAT 1 cut(s) 681
BsuRI GGCC 2 cut(s) 546, 942
BsuTUI ATCGAT 1 cut(s) 681
BtsCI GGATG 3 cut(s) 533, 869, 1042
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 4 cut(s) 129, 540, 612, 780
CciI TCATGA 2 cut(s) 205, 466
ClaI ATCGAT 1 cut(s) 681
CseI GACGC 2 cut(s) 158, 466
Csp6I GTAC 3 cut(s) 435, 1012, 1104
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 5 cut(s) 206, 269, 430, 467, 1015
CviQI GTAC 3 cut(s) 435, 1012, 1104
DdeI CTNAG 1 cut(s) 291
DriI GACNNNNNGTC 2 cut(s) 12, 388
EaeI YGGCCR 1 cut(s) 940
Eam1105I GACNNNNNGTC 2 cut(s) 12, 388
Eco130I CCWWGG 3 cut(s) 547, 995, 1062
Eco31I GGTCTC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 912
Eco57I CTGAAG 2 cut(s) 198, 270
EcoRII CCWGG 1 cut(s) 580
EcoRV GATATC 1 cut(s) 912
EcoT14I CCWWGG 3 cut(s) 547, 995, 1062
ErhI CCWWGG 3 cut(s) 547, 995, 1062
FaeI CATG 5 cut(s) 209, 272, 433, 470, 1018
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FatI CATG 5 cut(s) 205, 268, 429, 466, 1014
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 5 cut(s) 36, 166, 213, 840, 894
FokI GGATG 3 cut(s) 540, 876, 1049
Fsp4HI GCNGC 5 cut(s) 36, 166, 213, 840, 894
FspBI CTAG 1 cut(s) 200
GluI GCNGC 5 cut(s) 36, 166, 213, 840, 894
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 546, 942
HgaI GACGC 2 cut(s) 158, 466
Hin1II CATG 5 cut(s) 209, 272, 433, 470, 1018
HincII GTYRAC 2 cut(s) 721, 796
HindII GTYRAC 2 cut(s) 721, 796
HinfI GANTC 2 cut(s) 7, 979
HpaI GTTAAC 1 cut(s) 721
HphI GGTGA 3 cut(s) 116, 248, 358
Hpy166II GTNNAC 4 cut(s) 456, 721, 733, 796
Hpy188I TCNGA 1 cut(s) 114
Hpy8I GTNNAC 4 cut(s) 456, 721, 733, 796
Hpy99I CGWCG 2 cut(s) 152, 482
HpyAV CCTTC 5 cut(s) 173, 233, 530, 1094, 1121
HpyCH4III ACNGT 3 cut(s) 386, 460, 616
HpyCH4IV ACGT 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 58, 312, 638, 647, 810
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 5 cut(s) 209, 272, 433, 470, 1018
KspAI GTTAAC 1 cut(s) 721
LmnI GCTCC 4 cut(s) 94, 162, 505, 551
Lsp1109I GCAGC 4 cut(s) 22, 199, 826, 905
LweI GCATC 2 cut(s) 822, 905
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 4 cut(s) 140, 244, 854, 934
MbiI CCGCTC 1 cut(s) 165
MboII GAAGA 2 cut(s) 86, 337
MflI RGATCY 3 cut(s) 120, 172, 350
MluCI AATT 3 cut(s) 486, 725, 756
MlyI GAGTC 1 cut(s) 988
MmeI TCCRAC 2 cut(s) 598, 952
MnlI CCTC 8 cut(s) 72, 100, 105, 134, 202, 387, 628, 1133
MseI TTAA 2 cut(s) 405, 720
MslI CAYNNNNRTG 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 650
MspR9I CCNGG 1 cut(s) 582
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 6 cut(s) 41, 58, 312, 638, 647, 810
NlaIII CATG 5 cut(s) 209, 272, 433, 470, 1018
NlaIV GGNNCC 2 cut(s) 90, 174
NmeAIII GCCGAG 1 cut(s) 658
NmuCI GTSAC 3 cut(s) 140, 854, 934
PagI TCATGA 2 cut(s) 205, 466
PctI GAATGC 1 cut(s) 308
PkrI GCNGC 5 cut(s) 37, 167, 214, 841, 895
PleI GAGTC 1 cut(s) 987
PpsI GAGTC 1 cut(s) 987
Psp6I CCWGG 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 2 cut(s) 90, 174
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PsuI RGATCY 3 cut(s) 120, 172, 350
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 3 cut(s) 436, 1013, 1105
RsaNI GTAC 3 cut(s) 435, 1012, 1104
RseI CAYNNNNRTG 1 cut(s) 847
SaqAI TTAA 2 cut(s) 405, 720
SatI GCNGC 5 cut(s) 36, 166, 213, 840, 894
SchI GAGTC 1 cut(s) 988
ScrFI CCNGG 1 cut(s) 582
SfaNI GCATC 2 cut(s) 822, 905
SfcI CTRYAG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 847
SmlI CTYRAG 2 cut(s) 9, 96
SmoI CTYRAG 2 cut(s) 9, 96
Sse9I AATT 3 cut(s) 486, 725, 756
SsiI CCGC 3 cut(s) 38, 165, 789
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 580
StyI CCWWGG 3 cut(s) 547, 995, 1062
TaaI ACNGT 3 cut(s) 386, 460, 616
TaiI ACGT 1 cut(s) 446
TaqI TCGA 7 cut(s) 72, 392, 681, 774, 982, 1086, 1125
TaqII GACCGA 2 cut(s) 593, 865
TasI AATT 3 cut(s) 486, 725, 756
TatI WGTACW 1 cut(s) 1011
TauI GCSGC 1 cut(s) 168
Tru1I TTAA 2 cut(s) 405, 720
Tru9I TTAA 2 cut(s) 405, 720
TscAI CASTG 4 cut(s) 136, 547, 619, 787
TseFI GTSAC 3 cut(s) 140, 854, 934
TseI GCWGC 4 cut(s) 35, 212, 839, 893
Tsp45I GTSAC 3 cut(s) 140, 854, 934
TspDTI ATGAA 6 cut(s) 392, 418, 455, 711, 1031, 1053
TspGWI ACGGA 2 cut(s) 817, 912
TspRI CASTG 4 cut(s) 136, 547, 619, 787
XapI RAATTY 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 935
XmiI GTMKAC 1 cut(s) 455
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.