AT5G46170

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009867
Physical Location & Seq
Reverse (-)
18714864 .. 18717031
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46170.1

Sequence Viewer

Length: 1188 bp
ATGGCCGTAATTATCCCTCGTTCAGATCCACCGTCACGAATCCATCCGGAACCACCACAAACCCTAGAAATCGACCATTTCGATCATCTTCCTGACTCAATCCTCCTCCTCGTTTTCAACAAAATCGGCGACGTCAAAGCTCTCGGAAGATGCTGCGTCGTTTCCAGAAGATTCCATTCTCTCGTTCCTCAAGTCGATAACGTCGTTGTTCGTGTCGATTGCGTAATCTCCGACGACGATTCGTCATCTTTGTCATCTATCAAATCTCGCTCCGGTTCTTCCGCTGGTTCGTTCTCGGCTATATTTCGTCTTGTTGTTGGTGGCATTGTTAAGCCGTTACAAGCTTTAGGTCAATTCCTTGGTACGAAGAGATCATCATCATCATGTGGTGGTTCTGGATCTTCTTCTTCTTCGTTGTCTATTAGTGGAGATGATGATGGTGGAGAGATCGAACAAGGCGGTGTTACGCATCATTCACCTACTCAGGTTTTGAAAAACTTCGATGAGATTCGTTACTTAAGAATTGAGCTTCCTAGTGGTGAATTAGGGATTGATGATGGAGTTTTGTTGAAATGGAGAGCTGAATTTGGTTCTACTCTTGATAATTGTGTGATTCTCGGTGCTTCTTCAGTGATTCCACCAAATCCAATGAGAGTTTCTCAAGCTTGTGATACTACTACAGTCGTTGAAGCTCCCGGTTCTGGCTCCGATGATAATGGGAGTATACCGGAATCGTTTTACACAAACGGAGGTTTGAAATTGCGTGTTGTATGGACGATTAGCTCTTTGATTGCTGCATCAGCACGACATTACTTGTTACAACCGATAATCGCGGAGCATAAGACGCTTGATAGTTTAGTGCTCACTGATTCAGATGGGCAAGGTGTGCTCTGTATGAACAGAGATCAGCTAGAAGAGCTTAGGGTGAAACCATTAGCAGCTTCATCGGCTTCGAAGAGGACACTTGTGCCTGCTCTGAATATGAGACTATGGTATGCTCCAACCTTGGAATTGCCTGATGGAACTGTGTTAAAAGGTGCGACTTTGGTTGCGATAAGACCGAGTGAATCGAAAAAGGAAGTGTCTGATATCTCTTGGGTGTCTTCGGCTTTTGAGGAGCCTTATGAGACGGCTGCTAAGATGCTGGTTAAAAGAAGGACTTACTGTCTTGAAATGAACTCGTTCTAG

Protein Analysis

395

Amino Acids

42.49

Weight (kDa)

5.56

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 27 - 62 1.2e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30200 AT1G30200 AT4G18380 AT4G18380 AT5G46170
fragaria_vesca FvH4_3g05230
malus_domestica MD05G1281200.v1.1 MD10G1259600.v1.1
prunus_persica Prupe.4G083400_v2.0.a1
pyrus_communis pycom05g26130 pycom10g21600
rosa_chinensis RchiOBHm_Chr5g0014481
rosa_laevigata RLG00000032115
rosa_multiflora Rmu_sc0017039.1_g000005
rosa_roxburghii Rroxscaffold_1G00062070
rosa_rugosa Rorug05G0018400
rosa_samantha Rh5AG113700 Rh5BG110300 Rh5CG122200 Rh5DG109600
rosa_wichuraiana Rw5G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 724
AccII CGCG 1 cut(s) 833
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 3 cut(s) 282, 459, 833
AclWI GGATC 2 cut(s) 20, 406
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 701
AflII CTTAAG 1 cut(s) 517
AgsI TTSAA 6 cut(s) 118, 493, 571, 689, 757, 1172
AhdI GACNNNNNGTC 2 cut(s) 241, 1164
AjuI GAANNNNNNNTTGG 2 cut(s) 640, 672
Alw21I GWGCWC 2 cut(s) 864, 891
Alw26I GTCTC 2 cut(s) 979, 1121
AlwI GGATC 2 cut(s) 20, 406
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 153, 794, 938, 1133
ApoI RAATTY 1 cut(s) 584
Asp700I GAANNNNTTC 2 cut(s) 497, 1181
AsuC2I CCSGG 1 cut(s) 696
AsuHPI GGTGA 3 cut(s) 468, 551, 937
AsuII TTCGAA 1 cut(s) 953
BbsI GAAGAC 1 cut(s) 1095
Bbv12I GWGCWC 2 cut(s) 864, 891
BbvI GCAGC 4 cut(s) 140, 781, 950, 1120
BccI CCATC 5 cut(s) 51, 431, 551, 869, 1013
BceAI ACGGC 2 cut(s) 319, 1146
BcgI CGANNNNNNTGC 2 cut(s) 927, 961
BcnI CCSGG 1 cut(s) 696
BcoDI GTCTC 2 cut(s) 979, 1121
BfaI CTAG 4 cut(s) 65, 534, 911, 1186
BfmI CTRYAG 1 cut(s) 678
BfrI CTTAAG 1 cut(s) 517
BisI GCNGC 4 cut(s) 154, 795, 939, 1134
BlsI GCNGC 4 cut(s) 155, 796, 940, 1135
Bme1390I CCNGG 1 cut(s) 696
BmeRI GACNNNNNGTC 2 cut(s) 241, 1164
BmiI GGNNCC 3 cut(s) 51, 706, 1119
BmrFI CCNGG 1 cut(s) 696
BmsI GCATC 4 cut(s) 140, 478, 806, 1131
BpiI GAAGAC 1 cut(s) 1095
BplI GAGNNNNNCTC 2 cut(s) 643, 675
Bpu10I CCTNAGC 1 cut(s) 920
Bpu14I TTCGAA 1 cut(s) 953
BpuEI CTTGAG 2 cut(s) 174, 645
BpuMI CCSGG 1 cut(s) 696
BsaBI GATNNNNATC 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 358, 1005
BsaWI WCCGGW 3 cut(s) 46, 272, 727
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse8I GATNNNNATC 1 cut(s) 376
BseAI TCCGGA 1 cut(s) 46
BseDI CCNNGG 2 cut(s) 358, 1005
BseGI GGATG 1 cut(s) 43
BseJI GATNNNNATC 1 cut(s) 376
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 1 cut(s) 497
BseRI GAGGAG 3 cut(s) 95, 98, 1130
BseXI GCAGC 4 cut(s) 140, 781, 950, 1120
Bsh1236I CGCG 1 cut(s) 833
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 864, 891
BsiSI CCGG 4 cut(s) 47, 273, 696, 728
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 2 cut(s) 979, 1121
BsmBI CGTCTC 1 cut(s) 1121
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 953
Bsp1286I GDGCHC 2 cut(s) 864, 891
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 6 cut(s) 25, 82, 371, 398, 447, 904
BspACI CCGC 3 cut(s) 282, 459, 833
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 496
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 1 cut(s) 833
BspLI GGNNCC 3 cut(s) 51, 706, 1119
BspPI GGATC 2 cut(s) 20, 406
BspQI GCTCTTC 1 cut(s) 909
BspT104I TTCGAA 1 cut(s) 953
BspTI CTTAAG 1 cut(s) 517
BssECI CCNNGG 2 cut(s) 358, 1005
BssMI GATC 6 cut(s) 25, 82, 371, 398, 447, 904
BssNAI GTATAC 1 cut(s) 725
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 2 cut(s) 358, 1005
Bst1107I GTATAC 1 cut(s) 725
Bst4CI ACNGT 4 cut(s) 33, 682, 1027, 1166
Bst6I CTCTTC 3 cut(s) 362, 909, 950
BstACI GRCGYC 1 cut(s) 132
BstAFI CTTAAG 1 cut(s) 517
BstAPI GCANNNNNTGC 1 cut(s) 886
BstBI TTCGAA 1 cut(s) 953
BstC8I GCNNGC 1 cut(s) 972
BstDEI CTNAG 3 cut(s) 483, 920, 1137
BstF5I GGATG 1 cut(s) 43
BstFNI CGCG 1 cut(s) 833
BstKTI GATC 6 cut(s) 28, 85, 374, 401, 450, 907
BstMAI GTCTC 2 cut(s) 979, 1121
BstMBI GATC 6 cut(s) 25, 82, 371, 398, 447, 904
BstMWI GCNNNNNNNGC 5 cut(s) 800, 844, 886, 916, 947
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 1 cut(s) 678
BstUI CGCG 1 cut(s) 833
BstV1I GCAGC 4 cut(s) 140, 781, 950, 1120
BstV2I GAAGAC 1 cut(s) 1095
BstX2I RGATCY 2 cut(s) 25, 398
BstYI RGATCY 2 cut(s) 25, 398
BstZ17I GTATAC 1 cut(s) 725
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 636, 864
Cac8I GCNNGC 1 cut(s) 972
CseI GACGC 2 cut(s) 145, 853
Csp6I GTAC 1 cut(s) 363
CviAII CATG 1 cut(s) 384
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 3 cut(s) 483, 920, 1137
DpnI GATC 6 cut(s) 27, 84, 373, 400, 449, 906
DpnII GATC 6 cut(s) 25, 82, 371, 398, 447, 904
DriI GACNNNNNGTC 2 cut(s) 241, 1164
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 362, 909, 950
Eam1105I GACNNNNNGTC 2 cut(s) 241, 1164
EarI CTCTTC 3 cut(s) 362, 909, 950
Eco130I CCWWGG 2 cut(s) 358, 1005
Eco32I GATATC 1 cut(s) 1090
Eco57I CTGAAG 1 cut(s) 612
EcoRV GATATC 1 cut(s) 1090
EcoT14I CCWWGG 2 cut(s) 358, 1005
ErhI CCWWGG 2 cut(s) 358, 1005
Esp3I CGTCTC 1 cut(s) 1121
FaeI CATG 1 cut(s) 387
FalI AAGNNNNNCTT 2 cut(s) 1144, 1176
FatI CATG 1 cut(s) 383
FblI GTMKAC 1 cut(s) 724
Fnu4HI GCNGC 4 cut(s) 154, 795, 939, 1134
FokI GGATG 1 cut(s) 30
Fsp4HI GCNGC 4 cut(s) 154, 795, 939, 1134
FspBI CTAG 4 cut(s) 65, 534, 911, 1186
GluI GCNGC 4 cut(s) 154, 795, 939, 1134
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 47, 273, 696, 728
HgaI GACGC 2 cut(s) 145, 853
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 1 cut(s) 387
HindIII AAGCTT 2 cut(s) 342, 663
HpaII CCGG 4 cut(s) 47, 273, 696, 728
HphI GGTGA 3 cut(s) 468, 551, 937
Hpy166II GTNNAC 1 cut(s) 725
Hpy188I TCNGA 7 cut(s) 25, 146, 232, 709, 874, 978, 1087
Hpy188III TCNNGA 7 cut(s) 36, 47, 92, 165, 396, 599, 1169
Hpy8I GTNNAC 1 cut(s) 725
Hpy99I CGWCG 5 cut(s) 134, 161, 206, 236, 239
HpyAV CCTTC 1 cut(s) 1149
HpyCH4III ACNGT 4 cut(s) 33, 682, 1027, 1166
HpyCH4IV ACGT 2 cut(s) 132, 201
HpyCH4V TGCA 1 cut(s) 797
HpyF10VI GCNNNNNNNGC 5 cut(s) 800, 844, 886, 916, 947
HpyF3I CTNAG 3 cut(s) 483, 920, 1137
HpySE526I ACGT 2 cut(s) 132, 201
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 1 cut(s) 387
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 6 cut(s) 25, 82, 371, 398, 447, 904
LguI GCTCTTC 1 cut(s) 909
LmnI GCTCC 6 cut(s) 275, 697, 710, 835, 1003, 1117
Lsp1109I GCAGC 4 cut(s) 140, 781, 950, 1120
LweI GCATC 4 cut(s) 140, 478, 806, 1131
MaeI CTAG 4 cut(s) 65, 534, 911, 1186
MaeII ACGT 2 cut(s) 132, 201
MaeIII GTNAC 5 cut(s) 33, 336, 463, 512, 816
MalI GATC 6 cut(s) 27, 84, 373, 400, 449, 906
MboI GATC 6 cut(s) 25, 82, 371, 398, 447, 904
MflI RGATCY 2 cut(s) 25, 398
MhlI GDGCHC 2 cut(s) 864, 891
MluCI AATT 8 cut(s) 9, 353, 522, 542, 584, 604, 758, 1010
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 255, 1025
MnlI CCTC 8 cut(s) 27, 113, 116, 119, 198, 743, 951, 1108
MroI TCCGGA 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 497, 1181
MseI TTAA 4 cut(s) 330, 518, 1031, 1149
MslI CAYNNNNRTG 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 4 cut(s) 47, 273, 696, 728
MspR9I CCNGG 1 cut(s) 696
MvnI CGCG 1 cut(s) 833
MwoI GCNNNNNNNGC 5 cut(s) 800, 844, 886, 916, 947
NciI CCSGG 1 cut(s) 696
NdeII GATC 6 cut(s) 25, 82, 371, 398, 447, 904
NlaIII CATG 1 cut(s) 387
NlaIV GGNNCC 3 cut(s) 51, 706, 1119
NmeAIII GCCGAG 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 33
NspV TTCGAA 1 cut(s) 953
PciSI GCTCTTC 1 cut(s) 909
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 201
PdmI GAANNNNTTC 2 cut(s) 497, 1181
PfeI GAWTC 9 cut(s) 39, 171, 239, 508, 613, 634, 731, 869, 1067
PkrI GCNGC 4 cut(s) 155, 796, 940, 1135
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PspN4I GGNNCC 3 cut(s) 51, 706, 1119
PsuI RGATCY 2 cut(s) 25, 398
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 909
SaqAI TTAA 4 cut(s) 330, 518, 1031, 1149
SatI GCNGC 4 cut(s) 154, 795, 939, 1134
Sau3AI GATC 6 cut(s) 25, 82, 371, 398, 447, 904
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 696
SduI GDGCHC 2 cut(s) 864, 891
SfaNI GCATC 4 cut(s) 140, 478, 806, 1131
SfcI CTRYAG 1 cut(s) 678
SfuI TTCGAA 1 cut(s) 953
SmiMI CAYNNNNRTG 1 cut(s) 382
SmlI CTYRAG 3 cut(s) 189, 517, 660
SmoI CTYRAG 3 cut(s) 189, 517, 660
Sse9I AATT 8 cut(s) 9, 353, 522, 542, 584, 604, 758, 1010
SsiI CCGC 3 cut(s) 282, 459, 833
SspMI CTAG 4 cut(s) 65, 534, 911, 1186
StyD4I CCNGG 1 cut(s) 694
StyI CCWWGG 2 cut(s) 358, 1005
TaaI ACNGT 4 cut(s) 33, 682, 1027, 1166
TaiI ACGT 2 cut(s) 135, 204
TaqI TCGA 8 cut(s) 72, 81, 195, 216, 450, 501, 953, 1070
TaqII GACCGA 1 cut(s) 1075
TasI AATT 8 cut(s) 9, 353, 522, 542, 584, 604, 758, 1010
TfiI GAWTC 9 cut(s) 39, 171, 239, 508, 613, 634, 731, 869, 1067
Tru1I TTAA 4 cut(s) 330, 518, 1031, 1149
Tru9I TTAA 4 cut(s) 330, 518, 1031, 1149
TscAI CASTG 2 cut(s) 636, 871
TseFI GTSAC 1 cut(s) 33
TseI GCWGC 4 cut(s) 153, 794, 938, 1133
Tsp45I GTSAC 1 cut(s) 33
TspDTI ATGAA 2 cut(s) 911, 933
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 2 cut(s) 636, 871
Vha464I CTTAAG 1 cut(s) 517
XapI RAATTY 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 724
XmnI GAANNNNTTC 2 cut(s) 497, 1181
XspI CTAG 4 cut(s) 65, 534, 911, 1186
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.