AT5G47455

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000869
Physical Location & Seq
Forward (+)
19250659 .. 19252491
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47455.5

Sequence Viewer

Length: 303 bp
ATGGCTTCTAGATTCATGAGCAGATCCTCTGTCTCGTCTTTCAAATCCGCCATTAGATCTTCATTTCGAAATTCTCCCATTGGCACTGGATCTTCTCCGGCTGCTGCTTCATCCGCCGGATTTCGTATTCCGTCTAAACCAGCTGCTTCTCCTCTTCCCCGATTCTCCTTCTCCAGGTGTCCGTCGGAGCTTGGTTGTGTTCAGTCTCTGTTACCGCTACACAGCACGGTGGCGGCTGCGCGGTTGACGTCATGCTTAAGCACAACTTCCAGAAGCAGCCGAGCTCTCACTCAGGGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

10.36

Weight (kDa)

12.0

Isoelectric Point (pI)

81.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 251
AccII CGCG 1 cut(s) 241
AciI CCGC 5 cut(s) 48, 114, 215, 233, 241
AclWI GGATC 2 cut(s) 18, 97
AcsI RAATTY 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 174
AflII CTTAAG 1 cut(s) 256
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 3 cut(s) 143, 190, 284
AluI AGCT 3 cut(s) 143, 190, 284
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 2 cut(s) 37, 210
AlwI GGATC 2 cut(s) 18, 97
AlwNI CAGNNNCTG 1 cut(s) 208
ApeKI GCWGC 5 cut(s) 101, 104, 143, 236, 276
ApoI RAATTY 1 cut(s) 70
AspLEI GCGC 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 5 cut(s) 88, 91, 130, 223, 288
BciT130I CCWGG 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 37, 210
BfaI CTAG 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 256
BglII AGATCT 1 cut(s) 56
BisI GCNGC 6 cut(s) 102, 105, 144, 234, 237, 277
BlsI GCNGC 6 cut(s) 103, 106, 145, 235, 238, 278
Bme1390I CCNGG 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 175
BpmI CTGGAG 1 cut(s) 157
Bpu14I TTCGAA 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 1 cut(s) 91
BseBI CCWGG 1 cut(s) 175
BseGI GGATG 1 cut(s) 110
BseLI CCNNNNNNNGG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 299
BseXI GCAGC 5 cut(s) 88, 91, 130, 223, 288
Bsh1236I CGCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 286
BsiSI CCGG 2 cut(s) 98, 117
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 2 cut(s) 37, 210
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 286, 299
Bsp143I GATC 3 cut(s) 23, 56, 89
BspACI CCGC 5 cut(s) 48, 114, 215, 233, 241
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 1 cut(s) 15
BspPI GGATC 2 cut(s) 18, 97
BspT104I TTCGAA 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 256
BsrI ACTGG 1 cut(s) 91
BssMI GATC 3 cut(s) 23, 56, 89
BssNI GRCGYC 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 248
BstAFI CTTAAG 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 67
BstDEI CTNAG 1 cut(s) 291
BstENI CCTNNNNNAGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 241
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 3 cut(s) 26, 59, 92
BstMAI GTCTC 2 cut(s) 37, 210
BstMBI GATC 3 cut(s) 23, 56, 89
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 173
BstSLI GKGCMC 1 cut(s) 299
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 5 cut(s) 88, 91, 130, 223, 288
BstX2I RGATCY 3 cut(s) 23, 56, 89
BstYI RGATCY 3 cut(s) 23, 56, 89
BtsCI GGATG 1 cut(s) 110
BtsIMutI CAGTG 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 208
CciI TCATGA 1 cut(s) 15
CfoI GCGC 1 cut(s) 241
CviAII CATG 2 cut(s) 16, 252
CviJI RGCY 7 cut(s) 5, 101, 143, 190, 236, 279, 284
CviKI_1 RGCY 7 cut(s) 5, 101, 143, 190, 236, 279, 284
DdeI CTNAG 1 cut(s) 291
DpnI GATC 3 cut(s) 25, 58, 91
DpnII GATC 3 cut(s) 23, 56, 89
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 2 cut(s) 37, 103
Ecl136II GAGCTC 1 cut(s) 284
Eco24I GRGCYC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 284
EcoNI CCTNNNNNAGG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 173
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 2 cut(s) 19, 255
FaiI YATR 3 cut(s) 17, 253, 301
FalI AAGNNNNNCTT 2 cut(s) 250, 282
FatI CATG 2 cut(s) 15, 251
Fnu4HI GCNGC 6 cut(s) 102, 105, 144, 234, 237, 277
FokI GGATG 1 cut(s) 97
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 6 cut(s) 102, 105, 144, 234, 237, 277
FspBI CTAG 1 cut(s) 9
GlaI GCGC 1 cut(s) 240
GluI GCNGC 6 cut(s) 102, 105, 144, 234, 237, 277
GsuI CTGGAG 1 cut(s) 157
HapII CCGG 2 cut(s) 98, 117
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 2 cut(s) 19, 255
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 2 cut(s) 12, 162
HpaII CCGG 2 cut(s) 98, 117
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 3 cut(s) 9, 16, 270
Hpy8I GTNNAC 1 cut(s) 246
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 248
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 2 cut(s) 19, 255
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 3 cut(s) 23, 56, 89
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 8 cut(s) 72, 111, 130, 153, 160, 187, 278, 283
Lsp1109I GCAGC 5 cut(s) 88, 91, 130, 223, 288
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 1 cut(s) 210
MalI GATC 3 cut(s) 25, 58, 91
MboI GATC 3 cut(s) 23, 56, 89
MboII GAAGA 3 cut(s) 51, 84, 146
MflI RGATCY 3 cut(s) 23, 56, 89
MhlI GDGCHC 2 cut(s) 286, 299
MluCI AATT 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 2 cut(s) 37, 162
MseI TTAA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 143
MspCI CTTAAG 1 cut(s) 256
MspI CCGG 2 cut(s) 98, 117
MspR9I CCNGG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 1 cut(s) 113
NdeII GATC 3 cut(s) 23, 56, 89
NlaIII CATG 2 cut(s) 19, 255
NspV TTCGAA 1 cut(s) 67
PagI TCATGA 1 cut(s) 15
PfeI GAWTC 2 cut(s) 12, 162
PkrI GCNGC 6 cut(s) 103, 106, 145, 235, 238, 278
Psp124BI GAGCTC 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 173
PspGI CCWGG 1 cut(s) 173
PstNI CAGNNNCTG 1 cut(s) 208
PsuI RGATCY 3 cut(s) 23, 56, 89
PvuII CAGCTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 6 cut(s) 102, 105, 144, 234, 237, 277
Sau3AI GATC 3 cut(s) 23, 56, 89
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 2 cut(s) 286, 299
SetI ASST 5 cut(s) 145, 179, 192, 251, 286
SfuI TTCGAA 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 1 cut(s) 70
SsiI CCGC 5 cut(s) 48, 114, 215, 233, 241
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 286
StyD4I CCNGG 1 cut(s) 173
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 251
TaqI TCGA 1 cut(s) 67
TasI AATT 1 cut(s) 70
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 2 cut(s) 12, 162
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 1 cut(s) 91
TseI GCWGC 5 cut(s) 101, 104, 143, 236, 276
TspDTI ATGAA 3 cut(s) 4, 51, 99
TspGWI ACGGA 2 cut(s) 120, 171
TspRI CASTG 1 cut(s) 91
Vha464I CTTAAG 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 172
XapI RAATTY 1 cut(s) 70
XbaI TCTAGA 1 cut(s) 8
XspI CTAG 1 cut(s) 9
ZraI GACGTC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.