pycom04g05680

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000869
Physical Location & Seq
Forward (+)
5462201 .. 5462613
413 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05680.1

Sequence Viewer

Length: 309 bp
ATGGCTTCAGCCTGCAGGCAATTCGCCAACAGAGCTTCCCTATCGTCCATCCGATCCGCCGTCAGATCCAGCGTCCCAAAATCTTCCGCTTCACGCTTCCCTCTCCCCACCGCCTCCTCTCCCTCCTCCGCCCCTCCTCGACGTTTCTCGCTCTCCAGGTCTCCGGGTGCGTTGGGATGCGTTGAGTCACTACTTCCACTCCACAGCGCGGTGGCGGCGGCGAGGTTGACGTCGTCGCTGAGCACTACATCGAGGAGCTGCCGAGCGCTCTCACAGGGTACTCTCTGCTGCACCTCTCCCGGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

10.42

Weight (kDa)

11.76

Isoelectric Point (pI)

89.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 233
AccII CGCG 1 cut(s) 209
AciI CCGC 7 cut(s) 57, 87, 111, 129, 209, 215, 218
AclWI GGATC 2 cut(s) 48, 60
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 1 cut(s) 280
AfeI AGCGCT 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 208
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 2 cut(s) 35, 258
AluI AGCT 2 cut(s) 35, 258
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 2 cut(s) 48, 60
Aor51HI AGCGCT 1 cut(s) 267
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 2 cut(s) 258, 288
AspLEI GCGC 2 cut(s) 209, 268
AsuC2I CCSGG 2 cut(s) 165, 300
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 245, 275
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 1 cut(s) 157
BcnI CCSGG 2 cut(s) 165, 300
BcoDI GTCTC 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 13
BfoI RGCGCY 1 cut(s) 269
BisI GCNGC 4 cut(s) 216, 219, 259, 289
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 4 cut(s) 217, 220, 260, 290
Bme1390I CCNGG 3 cut(s) 157, 165, 300
BmrFI CCNGG 3 cut(s) 157, 165, 300
BmsI GCATC 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 139
Bpu1102I GCTNAGC 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 165, 300
BsaHI GRCGYC 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 1 cut(s) 208
BseBI CCWGG 1 cut(s) 157
BseGI GGATG 2 cut(s) 48, 182
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 230
BseRI GAGGAG 4 cut(s) 106, 115, 126, 268
BseXI GCAGC 2 cut(s) 245, 275
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 209
BshFI GGCC 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 2 cut(s) 164, 300
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 1 cut(s) 303
Bso31I GGTCTC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 2 cut(s) 53, 65
Bsp1720I GCTNAGC 1 cut(s) 239
BspACI CCGC 7 cut(s) 57, 87, 111, 129, 209, 215, 218
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 1 cut(s) 209
BspMAI CTGCAG 1 cut(s) 17
BspPI GGATC 2 cut(s) 48, 60
BspTNI GGTCTC 1 cut(s) 165
BssMI GATC 2 cut(s) 53, 65
BssNI GRCGYC 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 157
BstACI GRCGYC 1 cut(s) 230
BstC8I GCNNGC 2 cut(s) 13, 17
BstDEI CTNAG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 48, 182
BstFNI CGCG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 2 cut(s) 209, 268
BstKTI GATC 2 cut(s) 56, 68
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 53, 65
BstMWI GCNNNNNNNGC 2 cut(s) 32, 215
BstNI CCWGG 1 cut(s) 157
BstSCI CCNGG 3 cut(s) 155, 163, 298
BstSFI CTRYAG 1 cut(s) 13
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 2 cut(s) 245, 275
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 1 cut(s) 303
BtsCI GGATG 2 cut(s) 48, 182
Cac8I GCNNGC 2 cut(s) 13, 17
CfoI GCGC 2 cut(s) 209, 268
CseI GACGC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 279
CviJI RGCY 5 cut(s) 5, 11, 35, 258, 303
CviKI_1 RGCY 5 cut(s) 5, 11, 35, 258, 303
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 1 cut(s) 239
DpnI GATC 2 cut(s) 55, 67
DpnII GATC 2 cut(s) 53, 65
EciI GGCGGA 2 cut(s) 46, 118
Eco31I GGTCTC 1 cut(s) 165
Eco47III AGCGCT 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 155
FaqI GGGAC 1 cut(s) 59
Fnu4HI GCNGC 4 cut(s) 216, 219, 259, 289
FokI GGATG 2 cut(s) 35, 189
Fsp4HI GCNGC 4 cut(s) 216, 219, 259, 289
GlaI GCGC 2 cut(s) 208, 267
GluI GCNGC 4 cut(s) 216, 219, 259, 289
GsuI CTGGAG 1 cut(s) 139
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 303
HapII CCGG 2 cut(s) 164, 300
HgaI GACGC 1 cut(s) 61
HhaI GCGC 2 cut(s) 209, 268
Hin1I GRCGYC 1 cut(s) 230
Hin6I GCGC 2 cut(s) 207, 266
HinP1I GCGC 2 cut(s) 207, 266
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 1 cut(s) 185
HpaII CCGG 2 cut(s) 164, 300
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 2 cut(s) 53, 65
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 3 cut(s) 144, 235, 238
HpyCH4IV ACGT 2 cut(s) 142, 230
HpyCH4V TGCA 2 cut(s) 15, 291
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 215
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 2 cut(s) 142, 230
Hsp92I GRCGYC 1 cut(s) 230
HspAI GCGC 2 cut(s) 207, 266
Kzo9I GATC 2 cut(s) 53, 65
LmnI GCTCC 1 cut(s) 255
LpnPI CCDG 6 cut(s) 25, 82, 142, 169, 177, 260
Lsp1109I GCAGC 2 cut(s) 245, 275
LweI GCATC 1 cut(s) 167
MaeII ACGT 2 cut(s) 142, 230
MaeIII GTNAC 1 cut(s) 186
MalI GATC 2 cut(s) 55, 67
MboI GATC 2 cut(s) 53, 65
MboII GAAGA 1 cut(s) 75
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 1 cut(s) 20
MlyI GAGTC 1 cut(s) 194
MspI CCGG 2 cut(s) 164, 300
MspR9I CCNGG 3 cut(s) 157, 165, 300
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 32, 215
NciI CCSGG 2 cut(s) 165, 300
NdeII GATC 2 cut(s) 53, 65
NmeAIII GCCGAG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 186
PflFI GACNNNGTC 1 cut(s) 232
PkrI GCNGC 4 cut(s) 217, 220, 260, 290
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PstI CTGCAG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 65
PsyI GACNNNGTC 1 cut(s) 232
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SatI GCNGC 4 cut(s) 216, 219, 259, 289
Sau3AI GATC 2 cut(s) 53, 65
SbfI CCTGCAGG 1 cut(s) 17
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 157, 165, 300
SdaI CCTGCAGG 1 cut(s) 17
SduI GDGCHC 1 cut(s) 245
SetI ASST 7 cut(s) 37, 145, 161, 227, 233, 260, 296
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 1 cut(s) 13
Sse8387I CCTGCAGG 1 cut(s) 17
Sse9I AATT 1 cut(s) 20
SsiI CCGC 7 cut(s) 57, 87, 111, 129, 209, 215, 218
StyD4I CCNGG 3 cut(s) 155, 163, 298
TaiI ACGT 2 cut(s) 145, 233
TaqI TCGA 2 cut(s) 139, 251
TasI AATT 1 cut(s) 20
TauI GCSGC 2 cut(s) 218, 221
TseFI GTSAC 1 cut(s) 186
TseI GCWGC 2 cut(s) 258, 288
Tsp45I GTSAC 1 cut(s) 186
Tth111I GACNNNGTC 1 cut(s) 232
ZraI GACGTC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.