Prupe.5G068300_v2.0.a1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000869
Physical Location & Seq
Forward (+)
8247645 .. 8250140
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G068300.4

Sequence Viewer

Length: 303 bp
ATGGCTTCAGCCTGCAGGCAGTTCGCCAACAGAGCATCCCTATCGTCCGTCCGATCCGCCATCAGATCCAACGCCCCAAAATCTCCCACCTCACGCTTCCCTCTCCCGACATCCACTCCCTCAACTCCTCTTCGCCGTTCCTCACTCTCAAGGCCTCCGGGTGCACTGGGATGCGTGCAATCTCTACTGCCACTCCACAGCGCGGTGGCGGCGGCGAGAATGACGTCGTCTCTGAGCATCACATCGAGGAGTTGCCGTGCGCTCTCACAGGGTACTCTCTGCCGCACCTCTCCCGGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

10.4

Weight (kDa)

12.0

Isoelectric Point (pI)

82.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 227
AccII CGCG 1 cut(s) 203
AciI CCGC 5 cut(s) 57, 203, 209, 212, 283
AclWI GGATC 2 cut(s) 48, 60
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 1 cut(s) 202
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 2 cut(s) 48, 60
AoxI GGCC 2 cut(s) 152, 295
ApaLI GTGCAC 1 cut(s) 162
AspLEI GCGC 2 cut(s) 203, 262
AsuC2I CCSGG 2 cut(s) 159, 294
BaeGI GKGCMC 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 166
BccI CCATC 1 cut(s) 68
BceAI ACGGC 2 cut(s) 120, 240
BcgI CGANNNNNNTGC 4 cut(s) 4, 24, 38, 58
BcnI CCSGG 2 cut(s) 159, 294
BcoDI GTCTC 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 13
BisI GCNGC 3 cut(s) 210, 213, 283
BlsI GCNGC 3 cut(s) 211, 214, 284
Bme1390I CCNGG 2 cut(s) 159, 294
BmrFI CCNGG 2 cut(s) 159, 294
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 3 cut(s) 44, 161, 246
BmuI ACTGGG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 133
BpuMI CCSGG 2 cut(s) 159, 294
BsaHI GRCGYC 1 cut(s) 224
BsaXI ACNNNNNCTCC 6 cut(s) 100, 130, 177, 207, 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 35, 110, 176
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 117, 262
BseSI GKGCMC 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 2 cut(s) 154, 297
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 2 cut(s) 158, 294
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 234
BsmBI CGTCTC 1 cut(s) 234
BsnI GGCC 2 cut(s) 154, 297
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 53, 65
BspACI CCGC 5 cut(s) 57, 203, 209, 212, 283
BspANI GGCC 2 cut(s) 154, 297
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 203
BspMAI CTGCAG 1 cut(s) 17
BspPI GGATC 2 cut(s) 48, 60
BsrI ACTGG 1 cut(s) 171
BssMI GATC 2 cut(s) 53, 65
BssNI GRCGYC 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 13, 17, 176
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 3 cut(s) 35, 110, 176
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 2 cut(s) 203, 262
BstKTI GATC 2 cut(s) 56, 68
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 2 cut(s) 53, 65
BstMWI GCNNNNNNNGC 2 cut(s) 32, 209
BstSCI CCNGG 2 cut(s) 157, 292
BstSFI CTRYAG 1 cut(s) 13
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 2 cut(s) 154, 297
BtsCI GGATG 3 cut(s) 35, 110, 176
BtsIMutI CAGTG 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 13, 17, 176
CfoI GCGC 2 cut(s) 203, 262
Csp6I GTAC 1 cut(s) 273
CviJI RGCY 4 cut(s) 5, 11, 154, 297
CviKI_1 RGCY 4 cut(s) 5, 11, 154, 297
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 1 cut(s) 233
DpnI GATC 2 cut(s) 55, 67
DpnII GATC 2 cut(s) 53, 65
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 210, 213, 283
FokI GGATG 3 cut(s) 22, 97, 183
Fsp4HI GCNGC 3 cut(s) 210, 213, 283
GlaI GCGC 2 cut(s) 202, 261
GluI GCNGC 3 cut(s) 210, 213, 283
HaeIII GGCC 2 cut(s) 154, 297
HapII CCGG 2 cut(s) 158, 294
HhaI GCGC 2 cut(s) 203, 262
Hin1I GRCGYC 1 cut(s) 224
Hin6I GCGC 2 cut(s) 201, 260
HinP1I GCGC 2 cut(s) 201, 260
HpaII CCGG 2 cut(s) 158, 294
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 3 cut(s) 53, 65, 234
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 3 cut(s) 15, 164, 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 209
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 224
Hsp92I GRCGYC 1 cut(s) 224
HspAI GCGC 2 cut(s) 201, 260
Kzo9I GATC 2 cut(s) 53, 65
LpnPI CCDG 4 cut(s) 25, 152, 171, 254
LweI GCATC 3 cut(s) 44, 161, 246
MaeII ACGT 1 cut(s) 224
MalI GATC 2 cut(s) 55, 67
MboI GATC 2 cut(s) 53, 65
MboII GAAGA 1 cut(s) 122
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 8 cut(s) 100, 111, 130, 138, 151, 165, 240, 298
MslI CAYNNNNRTG 1 cut(s) 169
MspI CCGG 2 cut(s) 158, 294
MspR9I CCNGG 2 cut(s) 159, 294
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 32, 209
NciI CCSGG 2 cut(s) 159, 294
NdeII GATC 2 cut(s) 53, 65
PceI AGGCCT 1 cut(s) 154
PflFI GACNNNGTC 1 cut(s) 226
PkrI GCNGC 3 cut(s) 211, 214, 284
PstI CTGCAG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 65
PsyI GACNNNGTC 1 cut(s) 226
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 169
SatI GCNGC 3 cut(s) 210, 213, 283
Sau3AI GATC 2 cut(s) 53, 65
SbfI CCTGCAGG 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 159, 294
SdaI CCTGCAGG 1 cut(s) 17
SduI GDGCHC 1 cut(s) 166
SetI ASST 3 cut(s) 92, 227, 290
SfaNI GCATC 3 cut(s) 44, 161, 246
SfcI CTRYAG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse8387I CCTGCAGG 1 cut(s) 17
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 5 cut(s) 57, 203, 209, 212, 283
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 157, 292
TaiI ACGT 1 cut(s) 227
TaqI TCGA 1 cut(s) 245
TauI GCSGC 3 cut(s) 212, 215, 285
TscAI CASTG 1 cut(s) 171
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 1 cut(s) 171
Tth111I GACNNNGTC 1 cut(s) 226
VneI GTGCAC 1 cut(s) 162
ZraI GACGTC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.