AT5G47500

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012356
Physical Location & Seq
Reverse (-)
19270944 .. 19272892
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47500.1

Sequence Viewer

Length: 1089 bp
ATGGCGCAACTTACTAATTCCCTCAATTATCTCTTTTCTGTTTCTCTCTTATTATTTGTATCGTTCCACTGCTTATGTTTTCGTTTTTCGTTGGTTGCAGCTTGTTCAAACTCCACTGACGATCAACAGATTCAACACCATCACCACCGGAAATGGGTTGGTCCCTCAGGCCACAAAGTCATCACCGTCTCACTTAACGGCCACGCTCAGTTTCGCTCCGTCCAAGACGCTGTGGATTCCATACCAAAGAACAATAACAAGAGCATCACAATCAAGATTGCTCCCGGATTTTACAGAGAGAAAGTGGTGGTTCCAGCTACAAAACCGTACATAACGTTCAAAGGAGCTGGTAGGGATGTGACCGCTATAGAGTGGCACGACCGTGCGTCCGACCTTGGCGCTAACGGTCAACAGTTACGTACCTATCAAACCGCTTCCGTCACCGTCTACGCTAATTATTTCACCGCTAGAAACATTAGCTTCACGAATACTGCGCCGGCTCCATTGCCGGGGATGCAAGGGTGGCAAGCGGTGGCGTTTAGGATCTCCGGCGACAAAGCTTTCTTTTCCGGCTGCGGGTTTTACGGTGCACAAGACACTTTATGCGACGATGCTGGCCGTCATTACTTCAAGGAGTGTTACATTGAAGGCTCTATCGACTTTATCTTTGGTAATGGCCGCTCCATGTATAAAGATTGTGAGTTGCATTCGATAGCGTCAAGATTCGGGTCGATAGCGGCGCATGGTAGGACATGCCCGGAAGAGAAAACGGGTTTCGCGTTCGTGGGTTGTCGGGTAACAGGTACGGGTCCATTGTATGTGGGCCGGGCCATGGGACAATACTCACGTATCGTTTACGCCTACACTTACTTTGATGCTCTCGTCGCTCACGGTGGTTGGGACGATTGGGACCACAAATCCAACAAAAGCAAGACGGCATTTTTCGGAGTGTACAATTGCTATGGGCCAGGAGCAGCAGCGACGAGAGGCGTGTCTTGGGCTAGAGCTTTGGACTATGAATCGGCCCATCCATTTATCGCTAAGAGCTTCGTTAATGGGAGACATTGGATCGCTCCTCGAGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

362

Amino Acids

40.04

Weight (kDa)

8.99

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 61 - 353 3.2e-61 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47500
fragaria_vesca FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880
malus_domestica MD06G1058600.v1.1
prunus_persica Prupe.2G003200_v2.0.a1 Prupe.5G069600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0203261
rosa_laevigata RLG00000003539
rosa_multiflora Rmu_sc0020346.1_g000001
rosa_roxburghii Rroxscaffold_3G00253900
rosa_rugosa Rorug07G0075400
rosa_samantha Rh7AG204900 Rh7BG204300 Rh7CG215900 Rh7DG211500
rosa_wichuraiana Rw7G017840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 681
AccI GTMKAC 1 cut(s) 447
AccII CGCG 1 cut(s) 779
AciI CCGC 7 cut(s) 363, 432, 465, 530, 576, 679, 737
AclI AACGTT 1 cut(s) 335
AclWI GGATC 2 cut(s) 551, 1076
AcoI YGGCCR 3 cut(s) 199, 616, 676
AfaI GTAC 4 cut(s) 329, 421, 805, 953
AfiI CCNNNNNNNGG 4 cut(s) 154, 509, 576, 832
AgsI TTSAA 5 cut(s) 108, 134, 340, 631, 647
AhdI GACNNNNNGTC 1 cut(s) 385
AjnI CCWGG 1 cut(s) 967
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 7 cut(s) 101, 317, 347, 480, 560, 1007, 1047
AluI AGCT 7 cut(s) 101, 317, 347, 480, 560, 1007, 1047
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 2 cut(s) 193, 1054
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 2 cut(s) 551, 1076
Ama87I CYCGRG 1 cut(s) 1077
AoxI GGCC 8 cut(s) 169, 199, 616, 676, 823, 828, 965, 1023
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 4 cut(s) 98, 573, 974, 977
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
AspLEI GCGC 4 cut(s) 7, 401, 496, 742
AspS9I GGNCC 7 cut(s) 161, 809, 823, 828, 910, 965, 1024
AsuC2I CCSGG 4 cut(s) 285, 510, 758, 827
AsuHPI GGTGA 4 cut(s) 134, 175, 433, 454
AvaI CYCGRG 1 cut(s) 1077
AvaII GGWCC 3 cut(s) 161, 809, 910
AxyI CCTNAGG 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 832, 865
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 4 cut(s) 110, 560, 986, 989
BccI CCATC 2 cut(s) 147, 1035
BceAI ACGGC 3 cut(s) 214, 603, 951
BciT130I CCWGG 1 cut(s) 969
BcnI CCSGG 4 cut(s) 285, 510, 758, 827
BcoDI GTCTC 2 cut(s) 193, 1054
BfaI CTAG 2 cut(s) 468, 1002
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 6 cut(s) 99, 574, 679, 738, 975, 978
BlsI GCNGC 6 cut(s) 100, 575, 680, 739, 976, 979
Bme1390I CCNGG 5 cut(s) 285, 510, 758, 827, 969
Bme18I GGWCC 3 cut(s) 161, 809, 910
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 1077
BmgT120I GGNCC 7 cut(s) 161, 809, 823, 828, 910, 965, 1024
BmiI GGNNCC 5 cut(s) 163, 312, 501, 810, 911
BmrFI CCNGG 5 cut(s) 285, 510, 758, 827, 969
BmsI GCATC 5 cut(s) 273, 504, 601, 865, 1072
BpuMI CCSGG 4 cut(s) 285, 510, 758, 827
BsaAI YACGTR 2 cut(s) 419, 848
BsaJI CCNNGG 3 cut(s) 394, 509, 831
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 509, 576, 832
Bse118I RCCGGY 1 cut(s) 496
Bse21I CCTNAGG 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 1 cut(s) 969
BseDI CCNNGG 3 cut(s) 394, 509, 831
BseGI GGATG 3 cut(s) 361, 519, 1027
BseLI CCNNNNNNNGG 4 cut(s) 154, 509, 576, 832
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 2 cut(s) 180, 221
BseRI GAGGAG 1 cut(s) 1065
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 4 cut(s) 110, 560, 986, 989
Bsh1236I CGCG 1 cut(s) 779
Bsh1285I CGRYCG 1 cut(s) 382
BshFI GGCC 8 cut(s) 171, 201, 618, 678, 825, 830, 967, 1025
BsiEI CGRYCG 1 cut(s) 382
BsiHKAI GWGCWC 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 1077
BsiSI CCGG 8 cut(s) 148, 285, 497, 509, 549, 570, 758, 826
BslFI GGGAC 4 cut(s) 147, 849, 914, 923
BslI CCNNNNNNNGG 4 cut(s) 154, 509, 576, 832
BsmAI GTCTC 2 cut(s) 193, 1054
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 4 cut(s) 147, 849, 914, 923
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 8 cut(s) 171, 201, 618, 678, 825, 830, 967, 1025
BsoBI CYCGRG 1 cut(s) 1077
Bsp1286I GDGCHC 1 cut(s) 592
Bsp1407I TGTACA 1 cut(s) 951
Bsp143I GATC 3 cut(s) 121, 543, 1068
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 7 cut(s) 363, 432, 465, 530, 576, 679, 737
BspANI GGCC 8 cut(s) 171, 201, 618, 678, 825, 830, 967, 1025
BspCNI CTCAG 2 cut(s) 179, 220
BspFNI CGCG 1 cut(s) 779
BspLI GGNNCC 5 cut(s) 163, 312, 501, 810, 911
BspPI GGATC 2 cut(s) 551, 1076
BsrBI CCGCTC 1 cut(s) 681
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 496
BsrGI TGTACA 1 cut(s) 951
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 3 cut(s) 394, 509, 831
BssMI GATC 3 cut(s) 121, 543, 1068
BssT1I CCWWGG 2 cut(s) 394, 831
Bst2UI CCWGG 1 cut(s) 969
Bst4CI ACNGT 8 cut(s) 187, 327, 383, 407, 414, 445, 587, 893
Bst6I CTCTTC 1 cut(s) 756
BstAUI TGTACA 1 cut(s) 951
BstBAI YACGTR 2 cut(s) 419, 848
BstC8I GCNNGC 3 cut(s) 498, 528, 616
BstDEI CTNAG 3 cut(s) 166, 207, 1041
BstDSI CCRYGG 1 cut(s) 831
BstF5I GGATG 3 cut(s) 361, 519, 1027
BstFNI CGCG 1 cut(s) 779
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 4 cut(s) 7, 401, 496, 742
BstKTI GATC 3 cut(s) 124, 546, 1071
BstMAI GTCTC 2 cut(s) 193, 1054
BstMBI GATC 3 cut(s) 121, 543, 1068
BstMCI CGRYCG 1 cut(s) 382
BstMWI GCNNNNNNNGC 3 cut(s) 514, 523, 884
BstNI CCWGG 1 cut(s) 969
BstNSI RCATGY 1 cut(s) 756
BstSCI CCNGG 5 cut(s) 283, 508, 756, 825, 967
BstSFI CTRYAG 1 cut(s) 366
BstSLI GKGCMC 1 cut(s) 592
BstSNI TACGTA 1 cut(s) 419
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 4 cut(s) 110, 560, 986, 989
BstX2I RGATCY 1 cut(s) 543
BstYI RGATCY 1 cut(s) 543
Bsu36I CCTNAGG 1 cut(s) 166
BsuRI GGCC 8 cut(s) 171, 201, 618, 678, 825, 830, 967, 1025
BtgI CCRYGG 1 cut(s) 831
BtsCI GGATG 3 cut(s) 361, 519, 1027
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 67, 114
Cac8I GCNNGC 3 cut(s) 498, 528, 616
CfoI GCGC 4 cut(s) 7, 401, 496, 742
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 7 cut(s) 161, 809, 823, 828, 910, 965, 1024
CseI GACGC 3 cut(s) 236, 375, 705
Csp6I GTAC 4 cut(s) 328, 420, 804, 952
CviAII CATG 4 cut(s) 685, 743, 753, 832
CviQI GTAC 4 cut(s) 328, 420, 804, 952
DdeI CTNAG 3 cut(s) 166, 207, 1041
DpnI GATC 3 cut(s) 123, 545, 1070
DpnII GATC 3 cut(s) 121, 543, 1068
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 3 cut(s) 199, 616, 676
Eam1104I CTCTTC 1 cut(s) 756
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 756
Eco105I TACGTA 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 394, 831
Eco47I GGWCC 3 cut(s) 161, 809, 910
Eco81I CCTNAGG 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 1077
EcoRII CCWGG 1 cut(s) 967
EcoT14I CCWWGG 2 cut(s) 394, 831
ErhI CCWWGG 2 cut(s) 394, 831
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 4 cut(s) 688, 746, 756, 835
FaqI GGGAC 4 cut(s) 147, 849, 914, 923
FatI CATG 4 cut(s) 684, 742, 752, 831
FauI CCCGC 1 cut(s) 569
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 99, 574, 679, 738, 975, 978
FokI GGATG 3 cut(s) 368, 526, 1014
Fsp4HI GCNGC 6 cut(s) 99, 574, 679, 738, 975, 978
FspBI CTAG 2 cut(s) 468, 1002
GlaI GCGC 4 cut(s) 6, 400, 495, 741
GluI GCNGC 6 cut(s) 99, 574, 679, 738, 975, 978
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 8 cut(s) 171, 201, 618, 678, 825, 830, 967, 1025
HapII CCGG 8 cut(s) 148, 285, 497, 509, 549, 570, 758, 826
HgaI GACGC 3 cut(s) 236, 375, 705
HhaI GCGC 4 cut(s) 7, 401, 496, 742
Hin1II CATG 4 cut(s) 688, 746, 756, 835
Hin6I GCGC 4 cut(s) 5, 399, 494, 740
HinP1I GCGC 4 cut(s) 5, 399, 494, 740
HincII GTYRAC 1 cut(s) 410
HindII GTYRAC 1 cut(s) 410
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 4 cut(s) 130, 236, 723, 1019
HpaII CCGG 8 cut(s) 148, 285, 497, 509, 549, 570, 758, 826
HphI GGTGA 4 cut(s) 134, 175, 433, 454
Hpy166II GTNNAC 5 cut(s) 410, 448, 590, 856, 952
Hpy188I TCNGA 2 cut(s) 391, 947
Hpy188III TCNNGA 4 cut(s) 274, 484, 720, 1079
Hpy8I GTNNAC 5 cut(s) 410, 448, 590, 856, 952
Hpy99I CGWCG 3 cut(s) 611, 887, 985
HpyAV CCTTC 1 cut(s) 641
HpyCH4III ACNGT 8 cut(s) 187, 327, 383, 407, 414, 445, 587, 893
HpyCH4IV ACGT 3 cut(s) 335, 418, 847
HpyCH4V TGCA 4 cut(s) 98, 517, 590, 706
HpyF10VI GCNNNNNNNGC 3 cut(s) 514, 523, 884
HpyF3I CTNAG 3 cut(s) 166, 207, 1041
HpySE526I ACGT 3 cut(s) 335, 418, 847
Hsp92II CATG 4 cut(s) 688, 746, 756, 835
HspAI GCGC 4 cut(s) 5, 399, 494, 740
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
Kzo9I GATC 3 cut(s) 121, 543, 1068
LmnI GCTCC 7 cut(s) 221, 286, 344, 505, 686, 971, 1078
Lsp1109I GCAGC 4 cut(s) 110, 560, 986, 989
LweI GCATC 5 cut(s) 273, 504, 601, 865, 1072
MaeI CTAG 2 cut(s) 468, 1002
MaeII ACGT 3 cut(s) 335, 418, 847
MaeIII GTNAC 5 cut(s) 358, 414, 439, 638, 796
MalI GATC 3 cut(s) 123, 545, 1070
MbiI CCGCTC 1 cut(s) 681
MboI GATC 3 cut(s) 121, 543, 1068
MboII GAAGA 1 cut(s) 773
MfeI CAATTG 1 cut(s) 955
MflI RGATCY 1 cut(s) 543
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 4 cut(s) 16, 25, 454, 955
MmeI TCCRAC 2 cut(s) 414, 945
MnlI CCTC 4 cut(s) 32, 175, 980, 1086
MroNI GCCGGC 1 cut(s) 496
MseI TTAA 3 cut(s) 195, 1053, 1087
MslI CAYNNNNRTG 1 cut(s) 381
MspI CCGG 8 cut(s) 148, 285, 497, 509, 549, 570, 758, 826
MspR9I CCNGG 5 cut(s) 285, 510, 758, 827, 969
MunI CAATTG 1 cut(s) 955
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 1 cut(s) 969
MvnI CGCG 1 cut(s) 779
MwoI GCNNNNNNNGC 3 cut(s) 514, 523, 884
NaeI GCCGGC 1 cut(s) 498
NciI CCSGG 4 cut(s) 285, 510, 758, 827
NcoI CCATGG 1 cut(s) 831
NdeII GATC 3 cut(s) 121, 543, 1068
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 4 cut(s) 688, 746, 756, 835
NlaIV GGNNCC 5 cut(s) 163, 312, 501, 810, 911
NmuCI GTSAC 2 cut(s) 358, 439
NspI RCATGY 1 cut(s) 756
OliI CACNNNNGTG 1 cut(s) 381
PaeR7I CTCGAG 1 cut(s) 1077
PcsI WCGNNNNNNNCGW 1 cut(s) 776
PctI GAATGC 1 cut(s) 706
PdiI GCCGGC 1 cut(s) 498
PfeI GAWTC 4 cut(s) 130, 236, 723, 1019
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 6 cut(s) 100, 575, 680, 739, 976, 979
Ppu21I YACGTR 2 cut(s) 419, 848
Psp1406I AACGTT 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 967
PspGI CCWGG 1 cut(s) 967
PspN4I GGNNCC 5 cut(s) 163, 312, 501, 810, 911
PspPI GGNCC 7 cut(s) 161, 809, 823, 828, 910, 965, 1024
PsrI GAACNNNNNNTAC 2 cut(s) 320, 352
PsuI RGATCY 1 cut(s) 543
RsaI GTAC 4 cut(s) 329, 421, 805, 953
RsaNI GTAC 4 cut(s) 328, 420, 804, 952
RseI CAYNNNNRTG 1 cut(s) 381
SaqAI TTAA 3 cut(s) 195, 1053, 1087
SatI GCNGC 6 cut(s) 99, 574, 679, 738, 975, 978
Sau3AI GATC 3 cut(s) 121, 543, 1068
Sau96I GGNCC 7 cut(s) 161, 809, 823, 828, 910, 965, 1024
ScrFI CCNGG 5 cut(s) 285, 510, 758, 827, 969
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 5 cut(s) 273, 504, 601, 865, 1072
SfcI CTRYAG 1 cut(s) 366
Sfr274I CTCGAG 1 cut(s) 1077
SinI GGWCC 3 cut(s) 161, 809, 910
SlaI CTCGAG 1 cut(s) 1077
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 1077
SmoI CTYRAG 1 cut(s) 1077
SnaBI TACGTA 1 cut(s) 419
Sse9I AATT 4 cut(s) 16, 25, 454, 955
SsiI CCGC 7 cut(s) 363, 432, 465, 530, 576, 679, 737
SspMI CTAG 2 cut(s) 468, 1002
StyD4I CCNGG 5 cut(s) 283, 508, 756, 825, 967
StyI CCWWGG 2 cut(s) 394, 831
TaaI ACNGT 8 cut(s) 187, 327, 383, 407, 414, 445, 587, 893
TaiI ACGT 3 cut(s) 338, 421, 850
TaqI TCGA 4 cut(s) 657, 710, 731, 1078
TasI AATT 4 cut(s) 16, 25, 454, 955
TatI WGTACW 1 cut(s) 951
TauI GCSGC 2 cut(s) 681, 740
TfiI GAWTC 4 cut(s) 130, 236, 723, 1019
Tru1I TTAA 3 cut(s) 195, 1053, 1087
Tru9I TTAA 3 cut(s) 195, 1053, 1087
TscAI CASTG 2 cut(s) 74, 121
TseFI GTSAC 2 cut(s) 358, 439
TseI GCWGC 4 cut(s) 98, 573, 974, 977
Tsp45I GTSAC 2 cut(s) 358, 439
TspDTI ATGAA 1 cut(s) 1032
TspGWI ACGGA 2 cut(s) 208, 427
TspRI CASTG 2 cut(s) 74, 121
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 3 cut(s) 161, 809, 910
XceI RCATGY 1 cut(s) 756
XhoI CTCGAG 1 cut(s) 1077
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 2 cut(s) 468, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.