FvH4_5g18880

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012356
Physical Location & Seq
Forward (+)
10852215 .. 10855184
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18880.t2

Sequence Viewer

Length: 1113 bp
ATGGCTTCTCTTCCATCATCTTCATTCGTTTGTTTCTACTTCTTTGGTTTCATACTCACTTTGCTGTTGTTTCATGCAGAACCAACTCAAGCTAGAAGAAGCAGCTTTGATATTCTCAGCTCTCCTGCGACAGGAGATAGTAATAGTAGCAGCTCCTCCAACCATACCAGCAACCAGCGGAAGTGGGTGGGGCCGGTTGGCTACCTACACATTACCGTGGACATAAGAGGTTCCGGTGACTTCATGTCCGTCCAGTCCGCCGTCAATGCAGTTCCGGTCAACAATACGGTCAATGTTGTCATCCACATCAAGCCAGGATGTTACATAGAGAAAGTGGTGGTGCCCGCGACGAAGCCGTATATAACGTTTCAAGGAGCTGGGAAGGATGCGACGGTGATCGAATGGCACGACAGAGCCAGTGACCGTGGAACTAATGGACAGCAGCTGCGGACTTACAGAACCGCTTCTGTCACTGTGTTTGCCAACTATTTCTCAGCCAGAAACATTAGCTTCAAGAATACGGCACCGGCTCCGATGCCGGGGATGCAAGGATGGCAGGCGGTGGCGTTTCGGATATCGGGAGACAAGGCATACTTCTCCGGCTGCGGGTTCTATGGCGCCCAAGACACACTCTGCGACGACGTTGGACGCCATTACTTCAAGGAGTGTTACATCGAGGGTTCCATAGATTTCATCTTTGGAAATGGCCGCTCCATGTACAAAGACTGTGAGCTGCACTCAATAGCTACCAAGTTCGGCTCCATTGCTGCTCAGTTCAGAAAATCACCGGACGATAAATCGGGCTTTGCTTTTCTCAACTGCCGGGTAACGGGTAAGGGTCCACTCTATGTGGGTCGGGCCATGGGCAAGTACGCCAGGATCGTATACTCCTACACATACTTCGATGACGTGGTCGCCCATGGTGCCTGGGATGACTGGAACACAACTAGTACAAAGACTGATTTTCTAGGAGTGTACAAGTGCTGGGGTCCCGGAGCAGAAGCGTTGCGCGGTGTTCCATATGCCCCGGAGCTTGATTTCGACTCGGCGCACCGATTTCTGAGGAAGAGCTTCGTCAATGGAAGGCACTGGATATCCCCCTCTGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

371

Amino Acids

40.88

Weight (kDa)

8.78

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 71 - 172 2.4e-18 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47500
fragaria_vesca FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880 FvH4_5g18880
malus_domestica MD06G1058600.v1.1
prunus_persica Prupe.2G003200_v2.0.a1 Prupe.5G069600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0203261
rosa_laevigata RLG00000003539
rosa_multiflora Rmu_sc0020346.1_g000001
rosa_roxburghii Rroxscaffold_3G00253900
rosa_rugosa Rorug07G0075400
rosa_samantha Rh7AG204900 Rh7BG204300 Rh7CG215900 Rh7DG211500
rosa_wichuraiana Rw7G017840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 340, 523, 617, 923
AccBSI CCGCTC 1 cut(s) 711
AccI GTMKAC 1 cut(s) 885
AccII CGCG 2 cut(s) 347, 1011
AciI CCGC 9 cut(s) 178, 258, 345, 448, 462, 560, 606, 709, 1011
AclI AACGTT 1 cut(s) 365
AclWI GGATC 1 cut(s) 887
AcoI YGGCCR 1 cut(s) 706
AcyI GRCGYC 2 cut(s) 618, 649
AfaI GTAC 4 cut(s) 719, 872, 952, 977
AfiI CCNNNNNNNGG 4 cut(s) 131, 539, 606, 829
AgsI TTSAA 3 cut(s) 371, 514, 661
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 947
AjiI CACGTC 1 cut(s) 910
AjnI CCWGG 3 cut(s) 313, 875, 926
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 1 cut(s) 887
AlwNI CAGNNNCTG 1 cut(s) 445
AoxI GGCC 3 cut(s) 191, 706, 858
ApeKI GCWGC 7 cut(s) 102, 150, 442, 445, 603, 733, 767
Asp700I GAANNNNTTC 2 cut(s) 463, 1070
AspLEI GCGC 3 cut(s) 620, 1011, 1051
AspS9I GGNCC 4 cut(s) 191, 839, 858, 989
AsuC2I CCSGG 4 cut(s) 540, 824, 993, 1028
AsuHPI GGTGA 3 cut(s) 248, 406, 777
AvaII GGWCC 2 cut(s) 839, 989
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 4 cut(s) 340, 523, 617, 923
BbvI GCAGC 7 cut(s) 114, 162, 432, 454, 590, 720, 754
BccI CCATC 2 cut(s) 22, 546
BceAI ACGGC 3 cut(s) 245, 340, 537
BciT130I CCWGG 3 cut(s) 315, 877, 928
BcnI CCSGG 4 cut(s) 540, 824, 993, 1028
BcoDI GTCTC 1 cut(s) 576
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 3 cut(s) 93, 948, 968
BfoI RGCGCY 1 cut(s) 621
BisI GCNGC 8 cut(s) 103, 151, 443, 446, 604, 709, 734, 768
BlsI GCNGC 8 cut(s) 104, 152, 444, 447, 605, 710, 735, 769
Bme1390I CCNGG 7 cut(s) 315, 540, 824, 877, 928, 993, 1028
Bme18I GGWCC 2 cut(s) 839, 989
BmeRI GACNNNNNGTC 1 cut(s) 244
BmgBI CACGTC 1 cut(s) 910
BmgT120I GGNCC 4 cut(s) 191, 839, 858, 989
BmrFI CCNGG 7 cut(s) 315, 540, 824, 877, 928, 993, 1028
BmsI GCATC 4 cut(s) 376, 525, 534, 1096
BplI GAGNNNNNCTC 2 cut(s) 722, 754
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 4 cut(s) 540, 824, 993, 1028
BsaHI GRCGYC 2 cut(s) 618, 649
BsaJI CCNNGG 7 cut(s) 216, 424, 539, 861, 919, 927, 1026
BsaWI WCCGGW 3 cut(s) 233, 274, 787
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 539, 606, 829
Bse118I RCCGGY 2 cut(s) 193, 526
Bse1I ACTGG 4 cut(s) 253, 417, 941, 1094
Bse3DI GCAATG 1 cut(s) 762
BseBI CCWGG 3 cut(s) 315, 877, 928
BseDI CCNNGG 7 cut(s) 216, 424, 539, 861, 919, 927, 1026
BseGI GGATG 6 cut(s) 300, 323, 391, 549, 557, 937
BseLI CCNNNNNNNGG 4 cut(s) 131, 539, 606, 829
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 4 cut(s) 130, 507, 785, 1052
BseNI ACTGG 4 cut(s) 253, 417, 941, 1094
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 7 cut(s) 114, 162, 432, 454, 590, 720, 754
BseYI CCCAGC 2 cut(s) 377, 984
BsgI GTGCAG 1 cut(s) 719
Bsh1236I CGCG 2 cut(s) 347, 1011
BshFI GGCC 3 cut(s) 193, 708, 860
BshNI GGYRCC 4 cut(s) 340, 523, 617, 923
BslFI GGGAC 1 cut(s) 975
BslI CCNNNNNNNGG 4 cut(s) 131, 539, 606, 829
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 975
BsnI GGCC 3 cut(s) 193, 708, 860
Bsp1286I GDGCHC 1 cut(s) 345
Bsp1407I TGTACA 2 cut(s) 717, 975
Bsp143I GATC 2 cut(s) 396, 879
Bsp19I CCATGG 2 cut(s) 861, 919
BspACI CCGC 9 cut(s) 178, 258, 345, 448, 462, 560, 606, 709, 1011
BspANI GGCC 3 cut(s) 193, 708, 860
BspCNI CTCAG 4 cut(s) 129, 506, 784, 1053
BspFNI CGCG 2 cut(s) 347, 1011
BspPI GGATC 1 cut(s) 887
BspQI GCTCTTC 1 cut(s) 1061
BspT107I GGYRCC 4 cut(s) 340, 523, 617, 923
BsrBI CCGCTC 1 cut(s) 711
BsrDI GCAATG 1 cut(s) 762
BsrFI RCCGGY 2 cut(s) 193, 526
BsrGI TGTACA 2 cut(s) 717, 975
BsrI ACTGG 4 cut(s) 253, 417, 941, 1094
BssAI RCCGGY 2 cut(s) 193, 526
BssECI CCNNGG 7 cut(s) 216, 424, 539, 861, 919, 927, 1026
BssMI GATC 2 cut(s) 396, 879
BssNAI GTATAC 1 cut(s) 886
BssNI GRCGYC 2 cut(s) 618, 649
BssT1I CCWWGG 2 cut(s) 861, 919
Bst1107I GTATAC 1 cut(s) 886
Bst2UI CCWGG 3 cut(s) 315, 877, 928
Bst4CI ACNGT 6 cut(s) 217, 289, 394, 425, 475, 728
Bst6I CTCTTC 2 cut(s) 15, 1061
BstACI GRCGYC 2 cut(s) 618, 649
BstAUI TGTACA 2 cut(s) 717, 975
BstC8I GCNNGC 2 cut(s) 345, 558
BstDEI CTNAG 4 cut(s) 116, 493, 771, 1061
BstDSI CCRYGG 4 cut(s) 216, 424, 861, 919
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 6 cut(s) 300, 323, 391, 549, 557, 937
BstFNI CGCG 2 cut(s) 347, 1011
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 3 cut(s) 620, 1011, 1051
BstKTI GATC 2 cut(s) 399, 882
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 396, 879
BstMWI GCNNNNNNNGC 4 cut(s) 266, 544, 553, 923
BstNI CCWGG 3 cut(s) 315, 877, 928
BstSCI CCNGG 7 cut(s) 313, 538, 822, 875, 926, 991, 1026
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 2 cut(s) 347, 1011
BstV1I GCAGC 7 cut(s) 114, 162, 432, 454, 590, 720, 754
BstZ17I GTATAC 1 cut(s) 886
BsuRI GGCC 3 cut(s) 193, 708, 860
BtgI CCRYGG 4 cut(s) 216, 424, 861, 919
BtrI CACGTC 1 cut(s) 910
BtsCI GGATG 6 cut(s) 300, 323, 391, 549, 557, 937
BtsIMutI CAGTG 3 cut(s) 424, 471, 1087
Cac8I GCNNGC 2 cut(s) 345, 558
CaiI CAGNNNCTG 1 cut(s) 445
CfoI GCGC 3 cut(s) 620, 1011, 1051
Cfr10I RCCGGY 2 cut(s) 193, 526
Cfr13I GGNCC 4 cut(s) 191, 839, 858, 989
CseI GACGC 1 cut(s) 657
Csp6I GTAC 4 cut(s) 718, 871, 951, 976
CviAII CATG 5 cut(s) 74, 244, 715, 862, 920
CviQI GTAC 4 cut(s) 718, 871, 951, 976
DdeI CTNAG 4 cut(s) 116, 493, 771, 1061
DinI GGCGCC 1 cut(s) 619
DpnI GATC 2 cut(s) 398, 881
DpnII GATC 2 cut(s) 396, 879
DriI GACNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 706
Eam1104I CTCTTC 2 cut(s) 15, 1061
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 2 cut(s) 15, 1061
EciI GGCGGA 1 cut(s) 247
Eco130I CCWWGG 2 cut(s) 861, 919
Eco32I GATATC 2 cut(s) 576, 1095
Eco47I GGWCC 2 cut(s) 839, 989
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 989
EcoRII CCWGG 3 cut(s) 313, 875, 926
EcoRV GATATC 2 cut(s) 576, 1095
EcoT14I CCWWGG 2 cut(s) 861, 919
EgeI GGCGCC 1 cut(s) 619
EheI GGCGCC 1 cut(s) 619
ErhI CCWWGG 2 cut(s) 861, 919
FaeI CATG 5 cut(s) 77, 247, 718, 865, 923
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 975
FatI CATG 5 cut(s) 73, 243, 714, 861, 919
FauI CCCGC 2 cut(s) 352, 599
FauNDI CATATG 1 cut(s) 1021
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 8 cut(s) 103, 151, 443, 446, 604, 709, 734, 768
FokI GGATG 6 cut(s) 287, 330, 398, 556, 564, 944
Fsp4HI GCNGC 8 cut(s) 103, 151, 443, 446, 604, 709, 734, 768
FspBI CTAG 3 cut(s) 93, 948, 968
GlaI GCGC 3 cut(s) 619, 1010, 1050
GluI GCNGC 8 cut(s) 103, 151, 443, 446, 604, 709, 734, 768
GsaI CCCAGC 2 cut(s) 381, 988
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 3 cut(s) 193, 708, 860
HgaI GACGC 1 cut(s) 657
HhaI GCGC 3 cut(s) 620, 1011, 1051
Hin1I GRCGYC 2 cut(s) 618, 649
Hin1II CATG 5 cut(s) 77, 247, 718, 865, 923
Hin6I GCGC 3 cut(s) 618, 1009, 1049
HinP1I GCGC 3 cut(s) 618, 1009, 1049
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 1 cut(s) 1043
HphI GGTGA 3 cut(s) 248, 406, 777
Hpy166II GTNNAC 5 cut(s) 220, 280, 842, 886, 976
Hpy188I TCNGA 5 cut(s) 534, 573, 779, 1062, 1105
Hpy188III TCNNGA 2 cut(s) 514, 579
Hpy8I GTNNAC 5 cut(s) 220, 280, 842, 886, 976
Hpy99I CGWCG 4 cut(s) 352, 394, 641, 644
HpyAV CCTTC 2 cut(s) 376, 1077
HpyCH4III ACNGT 6 cut(s) 217, 289, 394, 425, 475, 728
HpyCH4IV ACGT 3 cut(s) 365, 642, 909
HpyCH4V TGCA 4 cut(s) 77, 269, 547, 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 544, 553, 923
HpyF3I CTNAG 4 cut(s) 116, 493, 771, 1061
HpySE526I ACGT 3 cut(s) 365, 642, 909
Hsp92I GRCGYC 2 cut(s) 618, 649
Hsp92II CATG 5 cut(s) 77, 247, 718, 865, 923
HspAI GCGC 3 cut(s) 618, 1009, 1049
KasI GGCGCC 1 cut(s) 617
KflI GGGWCCC 1 cut(s) 989
Kzo9I GATC 2 cut(s) 396, 879
LguI GCTCTTC 1 cut(s) 1061
LmnI GCTCC 7 cut(s) 158, 374, 535, 716, 764, 995, 1030
Lsp1109I GCAGC 7 cut(s) 114, 162, 432, 454, 590, 720, 754
LweI GCATC 4 cut(s) 376, 525, 534, 1096
MaeI CTAG 3 cut(s) 93, 948, 968
MaeII ACGT 3 cut(s) 365, 642, 909
MaeIII GTNAC 6 cut(s) 236, 320, 419, 469, 668, 826
MalI GATC 2 cut(s) 398, 881
MbiI CCGCTC 1 cut(s) 711
MboI GATC 2 cut(s) 396, 879
MboII GAAGA 3 cut(s) 12, 108, 1078
MhlI GDGCHC 1 cut(s) 345
Mly113I GGCGCC 1 cut(s) 618
MlyI GAGTC 1 cut(s) 1037
MmeI TCCRAC 2 cut(s) 183, 625
MnlI CCTC 5 cut(s) 166, 221, 670, 1056, 1111
MroXI GAANNNNTTC 2 cut(s) 463, 1070
MseI TTAA 1 cut(s) 1111
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 2 cut(s) 178, 445
MspR9I CCNGG 7 cut(s) 315, 540, 824, 877, 928, 993, 1028
MvaI CCWGG 3 cut(s) 315, 877, 928
MvnI CGCG 2 cut(s) 347, 1011
MwoI GCNNNNNNNGC 4 cut(s) 266, 544, 553, 923
NarI GGCGCC 1 cut(s) 618
NciI CCSGG 4 cut(s) 540, 824, 993, 1028
NcoI CCATGG 2 cut(s) 861, 919
NdeI CATATG 1 cut(s) 1021
NdeII GATC 2 cut(s) 396, 879
NlaIII CATG 5 cut(s) 77, 247, 718, 865, 923
NmeAIII GCCGAG 1 cut(s) 1025
NmuCI GTSAC 3 cut(s) 236, 419, 469
PciSI GCTCTTC 1 cut(s) 1061
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 879
PdmI GAANNNNTTC 2 cut(s) 463, 1070
PflFI GACNNNGTC 1 cut(s) 911
PfoI TCCNGGA 1 cut(s) 991
PkrI GCNGC 8 cut(s) 104, 152, 444, 447, 605, 710, 735, 769
PleI GAGTC 1 cut(s) 1037
PluTI GGCGCC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 1037
PpuMI RGGWCCY 1 cut(s) 989
Psp1406I AACGTT 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 989
Psp6I CCWGG 3 cut(s) 313, 875, 926
PspFI CCCAGC 2 cut(s) 377, 984
PspGI CCWGG 3 cut(s) 313, 875, 926
PspPI GGNCC 4 cut(s) 191, 839, 858, 989
PspPPI RGGWCCY 1 cut(s) 989
PstNI CAGNNNCTG 1 cut(s) 445
PsyI GACNNNGTC 1 cut(s) 911
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 4 cut(s) 719, 872, 952, 977
RsaNI GTAC 4 cut(s) 718, 871, 951, 976
RseI CAYNNNNRTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 1061
SaqAI TTAA 1 cut(s) 1111
SatI GCNGC 8 cut(s) 103, 151, 443, 446, 604, 709, 734, 768
Sau3AI GATC 2 cut(s) 396, 879
Sau96I GGNCC 4 cut(s) 191, 839, 858, 989
SchI GAGTC 1 cut(s) 1037
ScrFI CCNGG 7 cut(s) 315, 540, 824, 877, 928, 993, 1028
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 4 cut(s) 376, 525, 534, 1096
SfoI GGCGCC 1 cut(s) 619
SinI GGWCC 2 cut(s) 839, 989
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SpeI ACTAGT 1 cut(s) 947
SsiI CCGC 9 cut(s) 178, 258, 345, 448, 462, 560, 606, 709, 1011
SspDI GGCGCC 1 cut(s) 617
SspMI CTAG 3 cut(s) 93, 948, 968
StyD4I CCNGG 7 cut(s) 313, 538, 822, 875, 926, 991, 1026
StyI CCWWGG 2 cut(s) 861, 919
TaaI ACNGT 6 cut(s) 217, 289, 394, 425, 475, 728
TaiI ACGT 3 cut(s) 368, 645, 912
TaqI TCGA 4 cut(s) 399, 675, 903, 1041
TatI WGTACW 3 cut(s) 717, 950, 975
TauI GCSGC 1 cut(s) 711
Tru1I TTAA 1 cut(s) 1111
Tru9I TTAA 1 cut(s) 1111
TscAI CASTG 3 cut(s) 424, 478, 1094
TseFI GTSAC 3 cut(s) 236, 419, 469
TseI GCWGC 7 cut(s) 102, 150, 442, 445, 603, 733, 767
Tsp45I GTSAC 3 cut(s) 236, 419, 469
TspDTI ATGAA 5 cut(s) 12, 40, 62, 232, 682
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 3 cut(s) 424, 478, 1094
Tth111I GACNNNGTC 1 cut(s) 911
VpaK11BI GGWCC 2 cut(s) 839, 989
XagI CCTNNNNNAGG 1 cut(s) 129
XmiI GTMKAC 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 463, 1070
XspI CTAG 3 cut(s) 93, 948, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.